31results about How to "Osteoinductive" patented technology

The invention discloses a calcium phosphate bone induction biological ceramic stent based on a photocuring 3D printing technology and a preparation method thereof, and belongs to the field of biomedical materials. Through the method, ceramic paste which is high in content of solid, low in viscosity and good in dispersity and fluidity can be effectively prepared. Through the photocuring 3D printingtechnology and follow-up degreasing sintering, the bone induction porous calcium phosphate ceramic stent with an internal penetrating pore structure can be prepared, wherein the shape, size, porosityand macroscopic morphology of the bone induction porous calcium phosphate ceramic stent can be accurately controlled, and the bone induction porous calcium phosphate ceramic stent is used for bone tissue repairing and filling and individualized repairing.

The invention discloses a method for preparing a nanoparticle coating with the bone induction and antibiosis functions on the surface of a medical metal. According to the method, nanoparticles with different electric properties as used as a carrier, the nanoparticles are formed by mutual crosslinking of high-molecular polymers, and growth factors and antibiotics are respectively loaded in the process of preparing the nanoparticles with dissimilar electric properties, wherein factors and factor-friendly polyanions are sufficiently mixed to react first and are then immobilized into the nanoparticles through ionic crosslinking; and amino-containing antibiotics are grafted to a polyaldehyde anionic polymer through chemical crosslinking first and are then loaded into the nanoparticles through ionic crosslinking. The two types of nanoparticles are alternately assembled on the surface of the medical metal through electrostatic adsorption, covalent crosslinking is also formed among the particles, and the stability of the coating is enhanced. The coating prepared by using the method has the function of controlling adsorption and release of the growth factors and the antibiotics under the normal physiological environment, the activity of the growth factors are keep well and a strong bone induction function is achieved; and in addition, the antibiotics can be slowly released for a long time, and a good antibiosis effect is achieved.

The preparation process of sintered porous titanium artificial bone with bioceramic coating belongs to the field of biomedicine engineering technology. The preparation process includes stereo weaving of titanium metal fiber to form bionic structure model simulating human body's bone trabecula, pre-pressing formation and vacuum sintering to prepare porous titanium artificial bone, sol-gel process to form gradient and composite coating on the surface of the porous titanium artificial bone. The present invention can protect titanium metal skeleton, avoid titanium ion dissociation and make the artificial bone possess biological characteristic, and may be used in the clinical repair of large bone defect.

The invention relates to a method that a functional drug is enveloped into a polymeric material with biodegradability to form a nano-micron microsphere system. The method comprises that the polymeric material and simvastatin are dissolved in an organic liquor to form uniform dispersion which is then added into an liquor containing emulsifier Tween 80 and biologically nontoxic electrolytic polyvinyl alcohol or sodium dodecyl benzene sulfonate (SDBS), and then the obtained liquor is stirred, evaporated at a reduced pressure, centrifugalized, washed and vacuum dried, finally the simvastatin-contained delayed-release microsphere system is obtained. The surface of the microsphere is smooth and round, the granule thereof is regular without conglutination, and the granule diameter, the drug-loading rate (1-10 percent) and the encapsulation rate (above 40 percent) are all controllable, and the delayed-release time exceeds 2 months. The prepared simvastatin-contained delayed-release microsphere system can be processed into various preparations used in bony tissue absorption or bony defect parts, the microsphere system is degraded at a proper speed, thus the simvastatin can be further released; the degradation of the polymer can provide bony tissue with subsequent recuperating space to complete the repair of the bony defect parts.

The invention discloses a preparation method of a silk fibroin material of composite nano-grade hydroxyapatite and application of the silk fibroin material in repairing bone fracture parts. The preparation method comprises the following steps: uniformly dispersing nano-grade hydroxyapatite into silk fibroin in a certain ratio, dissolving the nano-grade hydroxyapatite with hexafluoroisopropanol, pouring a mixed solution into a columnar mold, soaking with methanol, performing self-assembling regeneration on a silk fibroin molecular chain so as to obtain a composite material which is excellent inmechanical strength, and finally manufacturing medicinal bone nails from the composite material by using a mechanical processing method. According to the characteristics that the material is good inbiocompatibility, excellent in mechanical property and good in in-vivo degradation controllability, the silk fibroin material can be applied to bone fracture fixation. A silk fibroin/nano-grade hydroxyapatite composite bone fracture internal fixation material with in-vivo degradation controllability can be prepared by using a method which is simple and feasible, high in finished product rate and free of toxicity, osteoporosis symptoms caused in the bone fracture repairing process can be effectively avoided, and because of the characteristic that the material does not need to be taken out through a second time of operation, bone fracture patients can be relieved from pain.

The invention relates to a beta-tricalcium phosphate porous ceramic drug-loading stent and a preparation method and an application thereof. The method comprises the following steps: (1) preparing thebeta-tricalcium phosphate porous ceramic drug-loading stent having macroscopic macropores and microscopic small pores therein; (2) preparing a silk fibroin solution, adding a drug to-be-loaded, stirring and mixing the materials uniformly, and obtaining a mixed solution of silk fibroin and the drug; (3) performing ultrasonic treatment of the above mixed solution, and then rapidly injecting the drug-loading silk fibroin solution into the beta-tricalcium phosphate porous ceramic stent after a period of time, after the completion of the perfusion, performing water bath at normal temperature, and waiting for solidifying the silk fibroin solution to gel; and (4) taking an above sample and performing freeze-drying treatment, removing excess silk fibroin on the surface of the beta-tricalcium phosphate porous stent. Compared with the prior art, the beta-tricalcium phosphate porous ceramic drug-loading stent has the beneficial effects that (1) good biocompatibility; (2) better bone repairing effect; and (3) sustained drug release effect.

The invention discloses an implant with a surface active coating. Screw threads are arranged on an outer side wall of the implant, and are sequentially divided into a cortical bone decompression screwthread section, a cortical bone cutting screw thread section, a cancellous bone extruding screw thread section and a cancellous bone cutting screw thread section from top to bottom; screw pitches ofthe screw threads are increased from top to bottom; a self-tapping groove is formed in the cancellous bone cutting screw thread section; a screw thread is arranged in the implant; a titanium-based bottom layer is arranged on the surface of the implant; a titanium dioxide layer containing calcium phosphate is covered on the outer surface of the titanium-based bottom layer; a polyelectrolyte layer is covered on the outer surface of the titanium dioxide layer containing the calcium phosphate. According to the implant with the surface active coating provided by the invention, the implant is provided with the four different screw threads so as to produce different lateral pressures on a cortical bone and a cancellous bone when screwing into a jaw bone, so that the initial stability of the implant is facilitated, the superior initial stability is favorable for osseointegration, and the surface of the implant is endowed with a favorable antibacterial property through polylysine bacteriostasis.

The invention discloses a degradable and resorbable porous magnesium compound biological factor cross pin for tissue engineering. The cross pin comprises a body which is cylindrical or rhombic magnesium alloy (1), part of the body (1) is provided with a plurality of porous regions used for containing biological factors with bone growth promoting or tendon differentiation effect, one end of the body is sharp and beneficial to cross transplantation of tendons in operation, and a host material of the body is magnesium or magnesium alloy and is subjected to surface modification. The cross pin can improve integration capability between the transplanted tendon and a bone tunnel to stimulate bone growth or induce bone mesenchymal stem cells (BMSC) in the bone tunnel to form tendon differentiation, and the process of tendon-bone healing is accelerated.

The invention relates to a method for preparing a polypeptide-grafted chitosan/hydroxyapatite/pearl powder bracket and belongs to the technical field of medical materials. The method comprises the following steps: firstly, dissolving chitosan into an acetic acid aqueous solution, dissolving 2-imino sulfane hydrochloride into the acetic acid aqueous solution, adjusting pH value, dialyzing and freeze-drying to obtain sulfhydrylated chitosan; dissolving bone morphogenetic protein-2 polypeptide into dimethyl sulfoxide, and adding into a sulfhydrylated chitosan aqueous solution to obtain polypeptide-grafted chitosan; at last, dissolving polypeptide-grafted chitosan into hydrochloric acid, adding pearl powder and hydroxyapatite powder, filling into a cylindrical mould, freeze-drying to obtain the polypeptide-grafted chitosan/hydroxyapatite/pearl powder bracket. The method has the advantages that BMP-2 polypeptide is grafted onto the chitosan through a chemical method. The bracket material has good osteoconduction, degradability and cell adhesion, meanwhile has osteoinduction as existence of the polypeptide and pearl powder organic matrix, and can be applied to bone defect repairing.

The present invention discloses a method for preparing bone grafting material by using velvet deerhorn and deer's bone as raw material and bone grafting material prepared by adopting said preparation method. It is characterized by that said invention utilizes the extract of velvet deerhorn and deer's cancellous bone to prepare bone-grafting material, its resource is rich and its application is safe, and said bone-grafting material contains several growth factors and velvet deerhorn polypeptide, so that it has obvious bone induction action, and can obtain extensive application.

The invention belongs to the technical field of composite bone scaffold preparation and provides a selective laser sintering preparation method by the utilization of nanometer 1393 bioglass and nano-diamond reinforced polyvinylidene fluoride. By the short degradation period of the 1393 bioglass, degradation of a polyvinylidene fluoride scaffold is promoted and the degradation speed is accelerated. By the high hardness and low granularity of nano-diamond, hardness and rigidity of the polyvinylidene fluoride scaffold are enhanced. Meanwhile, by excellent biological properties, bone inductivity of the polyvinylidene fluoride scaffold is enhanced. By the method, the nano-composite bone scaffold with good biological properties, controllable degradability and good mechanical property is prepared. The product is low-cost, has strong practicality, and has strong promotion and practical value.

The invention discloses a biocompatible composite bone repair material which is characterized by being prepared from the following raw materials in parts by weight: 15-20 parts of demineralized bone matrixes, 4-6 parts of hydroxyapatite, 6-8 parts of silk protein powder, 8-12 parts of shell powder, 3-5 parts of synthetic fiber, 1.2-1.5 parts of chitosan, 10-15 parts of bone morphogenetic proteins, 12-15 parts of a collagen solution and 10-12 parts of nanometer polylactic acid. The bone morphogenetic proteins in the biocompatible composite bone repair material can be used for inducing undifferentiated cells at the periphery of blood vessels to be converted to bones and cartilages so as to have a bone inducing effect; due to the addition of polylactic acid and other components with relatively good biocompatibility, bone cells can form new bones through surface creepage, and the long-term tensile strength can be kept; and due to the addition of hydroxyapatite, acidic products generated by polylactic acid degradation can be neutralized, the irritation effects of overhigh acidic products can be eliminated, and mineral substances can be provided for the growth of bone tissues.

The invention provides a bone-grafting filling material and a preparation method thereof. The method comprises the following steps: mixing and crushing biphasic calcium phosphates, calcium sulphate and iron oxide into grains of 0.1-1mm, burning at 800 DEG C for 3 hours, taking out to soak in 1M of (NH4)2HPO4 solution for 24 hours; drying two days, and burning at the temperature of 1100 DEG C for 1-2 days; slowly cooling, rinsing and baking, so as to obtain a skeleton structure; spraying a slow release formulation on a layer of collagen surface; spraying a layer of collagen on the surface of the slow release formulation to obtain a compound protein layer; baking the composite protein layer at 30 DEG C, carrying out superfine grinding, so as to obtain protein particles which are 50-100nm in particle size; finally, adding the skeleton structure and the protein particles to absolute ethyl alcohol; placing under the conditions at 1-5 DEG C and -0.01 to -0.05MPa for 2-3 days, so as to obtain the bone-grafting filling material. The bone-grafting filling material disclosed by the invention has biodegradability and biocompatibility, is free of or low in immunogenicity, strong in bone conductibility, bone inductivity, mechanical tolerance and the like, and kinds of performance requirements required by the bone filling material are completely met.

The invention relates to the technical field of biomedical materials, in particular to a drug sustained-release hydrogel semi-embedded composite scaffold and a preparation method thereof, and the preparation method comprises the following steps: (1) preparing an alendronate sodium deposition type hydroxyapatite scaffold; (2) preparing a pre-crosslinked drug sustained-release hydrogel solution; (3)sealing the holes in the lower layer of the hydroxyapatite scaffold with gelatin; (4) injecting the pre-crosslinked drug sustained-release hydrogel solution obtained in the step (2) into the upper layer of the hydroxyapatite scaffold, and carrying out photo-crosslinking; and (5) cleaning the hydrogel semi-embedded composite scaffold, and dissolving and removing the gelatin for sealing the holes in the lower layer of the scaffold to obtain the drug sustained-release hydrogel semi-embedded composite scaffold. The preparation method is convenient to operate and control, high in production efficiency, stable in product quality and beneficial to industrial production, and the prepared composite scaffold has the advantages of bionic structure, mechanical matching, high biological activity and capability of promoting targeted differentiation of stem cells.

The invention relates to a preparation method of silk fibroin composite bioglass tissue repair material and belongs to the field of biomedical materials. The preparation method includes preparation of silk fibroin solution, preparation of bioglass solution, and preparation of repair material by electrospinning. Silk fibroin can improve the mechanical properties of the material, bioglass can improve the medical properties of the material. The tissue repair material prepared has good mechanical properties, tissue repair properties and drug adhesion properties.

The invention belongs to the field of bone tissue replacement materials and particularly relates to a preparation method of adopting a chitosan-graphene-hydroxyapatite compound material as a bone tissue replacement material. The preparation method of the bone tissue replacement material includes the steps of preparing a hydroxyapatite/chitosan sizing agent, preparing a melamine sponge carrying thehydroxyapatite/chitosan sizing agent, conducting sintering molding, and conducting reduction of loaded graphene oxide and graphene oxide. A preparation method of the melamine sponge carrying the hydroxyapatite/chitosan sizing agent includes the steps of soaking the melamine sponge in the hydroxyapatite/chitosan sizing agent, extruding the melamine sponge to make pores in the melamine sponge filled with the sizing agent, and then taking the melamine sponge out for vacuum drying to obtain the melamine sponge carrying nano-hydroxyapatite/chitosan, wherein the vacuum temperature is 40-80 DEG C, and the vacuum degree is 60-90 KPa. The bone tissue replacement material is of a porous structure, has high mechanical intensity and high porosity and can meet the requirements of the bone tissue replacement material.

The invention discloses a preparation method of a chitin composite bio-glass tissue repair material and belongs to the field of biological medicine materials. The method comprises the steps of preparation of a chitin solution, preparation of a bioglass solution and electrostatic spinning to prepare a repair material. The chitin is capable of improving the mechanical property of the material and has antibacterial property; and the bioglass is capable of improving the medical property of the material. The prepared tissue repair material has good mechanical property, tissue repair performance and drug adhesiveness.