Preparation method of coating with bone induction and antibiosis functions on surface of medical metal

Abstract

The invention discloses a method for preparing a nanoparticle coating with the bone induction and antibiosis functions on the surface of a medical metal. According to the method, nanoparticles with different electric properties as used as a carrier, the nanoparticles are formed by mutual crosslinking of high-molecular polymers, and growth factors and antibiotics are respectively loaded in the process of preparing the nanoparticles with dissimilar electric properties, wherein factors and factor-friendly polyanions are sufficiently mixed to react first and are then immobilized into the nanoparticles through ionic crosslinking; and amino-containing antibiotics are grafted to a polyaldehyde anionic polymer through chemical crosslinking first and are then loaded into the nanoparticles through ionic crosslinking. The two types of nanoparticles are alternately assembled on the surface of the medical metal through electrostatic adsorption, covalent crosslinking is also formed among the particles, and the stability of the coating is enhanced. The coating prepared by using the method has the function of controlling adsorption and release of the growth factors and the antibiotics under the normal physiological environment, the activity of the growth factors are keep well and a strong bone induction function is achieved; and in addition, the antibiotics can be slowly released for a long time, and a good antibiosis effect is achieved.

Description

technical field [0001] The invention relates to a preparation method of a coating with osteoinductive and antibacterial effects on the surface of a medical metal. Background technique [0002] Due to their good biocompatibility and mechanical properties, medical metal materials have been successfully applied to the repair of bone defects and become important bone tissue replacement materials. Due to the biological inertness of the medical metal surface, new bone grows slowly on the surface, and the mechanical bond between the ingrown new bone and the surface is weak. Growth factors (such as BMPs, VEGF, FGF, etc.) play an important regulatory role in the process of bone repair. Loading BMP-2 on the surface of medical metal can improve its biological activity. However, growth factors are expensive, have a short half-life, are easy to denature, and are easy to dissipate in human tissues after local administration. Excessive input may have potential toxicity and carcinogenicity...

AI Technical Summary

Problems solved by technology

Existing methods mainly focus on direct covalent immobilization of factors on metal surfaces, but factor activity may be destroyed by covalent reactions

[0003] On the other hand, the surface of biomedical materials is easy to cause bacteria and other microorganisms to adhere to them. After implantation in the human body, infection will occur, and a second operation has to be performed to remove the infected implant, causing the patient to suffer. Severe and often life-threatening

Antibiotics can be immobilized on the surface of the material to reduce infection, but the existing immobilization method is a physical adsorption method, and the antibiotics are easy to be released in a short period of time, and the antibacterial effect time is short and the antibacterial effect is poor