612 results about "Sodium periodate" patented technology

The invention relates to a biocolloid hemostatic prepared by aldehyde-modified sodium alginate and amine-modified gelatine. Sodium alginate and gelatine as raw materials; according to ratio, aldehyde-modified sodium alginate solution with the concentration of 5-25% and the same volume of amine-modified gelatine solution with the concentration of 5-40% are mixed; sodium alginate is modified by aldehyde through sodium periodate oxidation; and then gel is obtained by mixing with gelatine modified by ethanediamine. The adopted gelatine and sodium alginate are safe and stable, have good biocompatibility and can be absorbed and degraded by organism. Gel is rapidly formed through Schiff base reaction of aldehyde-modified sodium alginate and amine-modified gelatine, and the gel has the same gel-forming time as fibrin glue but better adhesion property and adhesion strength, can be acted on any part of body through simple local spraying or injection, and can be used as substitute product of fibrin glue.

The invention relates to a preparation method of injectable natural polysaccharide self-healing hydrogel. The preparation method of the injectable natural polysaccharide self-healing hydrogel comprises the following steps: firstly synthesizing water soluble N-carboxyethyl chitosan by carrying out a Michael addition reaction on acrylic acid and chitosan, carrying out an oxidization reaction on sodium periodate and sodium alginate to obtain oxidized sodium alginate, then carrying out a dehydration condensation reaction on N-carboxyethyl chitosan and oxidized sodium alginate and on adipic dihydrazide and oxidized sodium alginate in a phosphate buffer liquid at the temperature of 37 DEG C, so as to respectively generate invertible imine bond and acylhydrazone bond, and finally preparing injectable natural polysaccharide macromolecular hydrogel with self-healing property. The preparation method of the injectable natural polysaccharide self-healing hydrogel has the advantages that a synthetic process of the injectable self-healing hydrogel is simple and green, reaction conditions are mild, the injectable self-healing hydrogel can form gel in a physiological environment and has self-healing capability, and the injectable self-healing hydrogel has a good application prospect in the fields of drug sustained release and the like.

The invention discloses a bio-medical adhesive, which can be cross-linked with a wound in situ, and a preparation method of the bio-medical adhesive. The preparation method is characterized by comprising the steps of first, preparing oxidized sodium alginate by using sodium periodate to oxidize sodium alginate; dissolving the oxidized sodium alginate in an MES (Methyl Ester Sulfonate) buffer solution, dissolving 1-(3-dimethyl aminopropyl)-3-ethyl carbodiimide hydrochloride and N-hydroxyl succinimide in the MES buffer solution as well under nitrogen protection, continuously adding dopamine, carrying out dialyzing and freeze-drying after reacting for 8 to 24 hours, and obtaining A liquid by dissolving a reaction product in a PBS (Phosphate Buffer Solution) or a sodium borate solution; then, dissolving I-shaped collagen in an acid solution, and obtaining B liquid by neutralizing the solution pH (potential of Hydrogen) to be 5 to 8; firstly pre-treating the wound by using hydrogen peroxide or HRP (Horse Radish Peroxidase) before the bio-medical adhesive is used, then immediately coating the wound with the bio-medical adhesive after mixing the A liquid with the B liquid, and forming gel in 30 to 120 s, wherein the gel is firmly adhered to the surface of the wound. The bio-medical adhesive disclosed by the invention has a stronger adhesive force under a moist environment in vivo, integrates filling, sealing and bleeding stopping functions, is good in biocompatibility, is bio-degradable and can be used for promoting wound healing.

Disclosed is a method for making full-length CDNA libraries, which is for making libraries of cDNAs having a length corresponding to a full-length of mRNAs and comprises the following steps of; binding a tag molecule to a diol structure present in 5' Cap (7MeGpppN) sites of mRNAs, forming RNA-DNA hybrids by reverse transcription using primers such as oligo dT and the mRNAs connected with the tag molecule as templates, and separating RNA-DNA hybrids carrying a DNA corresponding to a full-length of mRNAs from the RNA-DNA hybrids formed above by using function of the tag molecule. To obtain mRNA connected with a tag molecule, the diol structure present in 5' Cap site of mRNA is subjected to a ring-open reaction by oxidation with sodium periodate to form a dialdehyde and the dialdehyde is reacted with a tag molecule having a hydrazine terminus. According to the present invention, there are provided a novel method capable of efficiently labeling 5' Cap site and a method for making full-length cDNA libraries utilizing the labeling method.

The invention discloses a linen fiber modification method, in particular to a linen fiber graft aminization modification craft, belonging to the technical field of printing and dyeing, which is characterized in that the linen fiber is selectively oxidized with oxidant solution such as sodium periodate, and oxidation linen fiber is obtained; the surface aldehyde group of the oxidation linen fiber is reacted with the surface amino of amine-terminated overbranched compound and the quaternary ammonium salt, which are combined by covalent bond, and the graft aminization linen fiber is prepared; the abundant amino, imino group and quaternary ammonium branched chain are introduced on the surface of obtained fiber, which can increase significantly the dyeing performance of linen fiber, and simultaneously increase the pliability softness, hygroscopicity and antibacterial performance of linen fiber. The linen fiber modification method has the advantages of fast reaction rate, high graft efficiency, good fastness, convenient and easy operation, realization of non-salt dyeing for linen fiber after modification treatment, environment protection, simplified normal dyeing craft and wide potential application.

The invention discloses a multifunctional cardiovascular coating material with super-hydraulic performance and a preparation method thereof. The method comprises the following steps that (1) a substrate material is subjected to pretreatment; (2) the pretreated substrate material is put into a buffer system; then, a polyphenol compound and dopamine are added; reaction is performed for 2h at 4 to 50DEG C; then, sodium periodate is added; reaction is performed at 4 to 50 DEG C; (3) the product obtained in the step (2) is subjected to ultrasonic cleaning by deionized water for 3 to 5 times; nitrogen gas is used for drying; the multifunctional cardiovascular coating material is obtained. A prepared multifunctional cardiovascular coating has good stability, excellent hydrophilicity, good anticoagulant performance, good anti-inflammatory performance and good antioxidation performance; the endothelial cell apoptosis is avoided; the capability of inhibiting the smooth muscle cell multiplication is realized; the multifunctional cardiovascular coating material can be used for preparation of blood contact type materials such as intravascular stents, cardiac valves, artificial blood vessels and blood contact catheters in the field of medical materials.

The invention relates to chemical crosslinking type glucan hydrogel and a preparation method thereof, and belongs to the technical field of biomedical functional polymer materials. The preparation method comprises the steps that glucan is dissolved in water at room temperature and then placed in an ice-water bath, and sodium periodate is added to obtain a mixed solution; the mixed solution is stirred for reaction for 2-6 h on the room temperature and dark conditions; the stirred solution is dialyzed with a regenerated cellulose dialysis bag in deionized water, and after dialysis is completed, freeze drying is performed to obtain partial hydroformylation glucan solids with the hydroformylation degree of 40-60 percent; the obtained partial hydroformylation glucan solids are dissolved in the deionized water to obtain an aqueous hydroformylation glucan solution, a multi-amino crosslinking agent is added in the aqueous hydroformylation glucan solution to be mixed evenly at the room temperature, and then the glucan hydrogel is prepared. The chemical crosslinking type glucan hydrogel is prepared by taking natural glucan macromolecules with the good biosafety as a material basis through chemical crosslinking of the partial hydroformylation glucan and the multi-amino crosslinking agent.

The invention discloses a dendritic cellulose-based amphoteric flocculating-decolorizing agent and a preparation method of the dendritic cellulose-based amphoteric flocculating-decolorizing agent. The dendritic cellulose-based amphoteric flocculating-decolorizing agent is characterized in that a cellulosic material is mainly taken as a molecular framework; the cellulosic material is oxidized into dialdehyde cellulose by utilization of sodium periodate; then an aldehyde group and an amino group are used to form a Schiff-base structure; the flocculating-decolorizing agent is formed by means of bonding and grafting polyethyleneimine; the cellulosic material, the sodium periodate and the polyethyleneimine are sequentially added for preparation by means of taking water as a solvent; the surface charge characteristic and active site of the flocculating-decolorizing agent are regulated by means of changing a mass ratio of the cellulosic material to the polyethyleneimine. The dendritic cellulose-based amphoteric flocculating-decolorizing agent has the advantages of high flocculation efficiency, stable effect, environmental friendliness, and efficient application in purification treatment of complex water body, such as papermaking, printing and dyeing, heavy metal containment and the like.

The invention belongs to the technical field of biomass nanometer materials and discloses a nano-crystalline cellulose fiber high in carboxyl group content and preparation and application thereof. A preparation method comprises the steps of preheating fiber slurry for 0.5-1.5h with sodium periodate at the temperature of 50-60 DEG C; adding a sodium chlorite solution and acetum into the slurry to be reacted for 1-3h at the temperature of 30-50 DEG C, adding TEMPO, NaClO2 and NaClO, and performing microwave heating to the temperature of 55-65 DEG C to enable the mixture to be reacted for 1-3h; performing ultrasonic dispersion and freeze drying to obtain the nano-crystalline cellulose fiber high in carboxyl group content. According to the nano-crystalline cellulose fiber, hydroxyl at the positions of C2 and C3 of cellulose is oxidized into a formyl group which is oxidized into carboxyl by sodium chlorite, hydroxyl at the position of C6 is selectively oxidized into carboxyl through a TEMPO neutral oxidation system, and accordingly the total carboxyl group content of the fiber is greatly increased, and obtained products can be used for advanced treatment of waste water at the middle section of paper making.

The invention discloses a preparation method of a highly antibacterial alginate dressing, and belongs to the technical field of preparation of medical dressings. The preparation method comprises the following steps: oxidizing sodium alginate by virtue of sodium periodate, and mixing silver nitrate, zinc nitrate, a trisodium citrate solution and the like so as to obtain a silver-zinc antibacterial agent; then, combining dopamine hydrochloride and the oxidized sodium alginate, wherein the dopamine hydrochloride is attached to the surface of the oxidized sodium alginate through a dopamine polymerization reaction, and on the basis of reducing and chelating effects of the dopamine on silver and zinc irons, silver and zinc are uniformly loaded on the surface of the oxidized sodium alginate by virtue of a method of conducting in-situ reduction on the silver and zinc irons, so that a spinning solution is prepared; and conducting vacuum deaerating, spinning, coagulating, stretching, rinsing, rolling and drying, and making the dressing by virtue of a needle punching technology, so that the highly antibacterial alginate dressing is obtained. The alginate dressing prepared by the invention is excellent in hemostatic and antibacterial effects, the alginate dressing is good in hygroscopic swelling performance and the alginate dressing is capable of effectively protecting wounds and accelerating healing speed of the wounds; and dressing fibers are closely combined, and the alginate dressing is relatively good in mechanical performance and convenient to change.

The invention relates to a method for selective enrichment and identification of N-glycosylation sites in a biological sample based on a reductive amination reaction of amino and aldehyde groups and application of the method to proteomic analysis of N-glycoprotein. The method comprises the following steps: taking a biological sample containing glycoprotein, carrying out enzymatic hydrolysis by using trypsin and then blocking the terminated amino group and side-chain amino group of a peptide fragment by using formaldehyde; then oxidizing a sugar chain on glycopeptide by using a sodium periodate solution so as to produce an aldehyde group; then enriching oxidized glycopeptide with amino microspheres and carrying out treatment with peptide glycosidase PNGase F; and carrying out mass spectrometry on released N-glycopeptide so as to obtain proteome information of N-glycoprotein in the sample. The method can be used for proteomic analysis of glycosylation and can acquire identification results of corresponding glycoprotein, glycopeptide and glycosylation sites at the same time. The method is simple and highly efficient and has high throughput in identification of N-glycosylation sites on glycoprotein.

The invention relates to a finishing method of an antibacterial cotton fabric. The finishing method comprises the following steps: performing light-shielded oxidation reaction on cotton fibers put into a sodium periodate solution with certain concentration to obtain oxidized cotton fibers; dipping the oxidized cotton fibers into a chitosan solution with certain concentration for reacting to obtain chitosan grafted cotton fibers; and finally, dipping the chitosan grafted cotton fibers into a silver nitrate solution with certain concentration for a certain period of time, dipping twice and rolling twice, and heating the fibers in a 600W microwave oven for 8 minutes to obtain the antibacterial cotton fabric. According to the invention, the cotton fabric is taken as a base material, and the nano-silver antibacterial cotton fabric is generated by chitosan in situ through a microwave radiation method and is excellent in antibacterial and washable performance.

The invention discloses a method for preparing hemostatic cotton through hyaluronic acid crosslinked gelatin and belongs to the field of biomedical materials. The method includes the steps that firstly, a novel hydroformylation hyaluronic acid crosslinking agent is prepared; then, the hydroformylation hyaluronic acid crosslinking agent is mixed with gelatin for foaming, refrigerating and drying are conducted, and the gelatin hemostatic cotton can be obtained after sterilization. According to the method, strong oxidant sodium periodate is utilized to oxidize a carbon-carbon bond of cis diol in the hyaluronic acid so that the hyaluronic acid can react with amidogen for formylation; the hyaluronic acid obtained after formylation serves as a macromolecular crosslinking agent and reacts with the gelatin containing an amino functional group, and the gelatin is made to form a cross-linked structure, so that mechanical performance is enhanced, and the sponge function is given to the gelatin. The gelatin is good in biocompatibility, has the hemostatic and anti-microbial functions and serves as a sponge base material, the hydroformylation hyaluronic acid serves as the crosslinking agent, the prepared hemostatic cotton is good in mechanical performance, safe and non-toxic, the hemostatic property is remarkably improved, a good pain relieving function is achieved, and the effect of promoting healing of injured tissues is achieved.

The invention discloses a thickened oil well polymer plugging remover. The plugging remover is prepared from the following materials in percentage by mass: 0.5 to 5.0 percent of gel breaker, 1.0 to 10.0 percent of cleaning agent, 0.1 to 5.0 percent of clay stabilizer, 0.1 to 5.0 percent of iron ion stabilizer, 0.1 to 5.0 percent of surfactant, 0.1 to 5.0 percent of corrosion inhibitor, 0.1 to 5.0 percent of bactericide and the balance of water, wherein the gel breaker is prepared from 30 to 70 percent of gel breaker I and 30 to 70 percent of gel breaker II, the gel breaker I is one or more of ammonium persulfate, sodium thiosulfate, potassium periodate and potassium monopersulfate, and the gel breaker II is one or more of calcium peroxide, potassium peroxodisulfate, sodium periodate and iron sulfide. The thickened oil well polymer plugging remover is suitable for solving the plugging problem caused by a thickened oil well polymer, has a better plugging removal effect than a conventional single oxidizer or single acid liquor, and has the advantages of good plugging removal effect, strong corrosion resistance, long period of validity, safety and the like.

The invention discloses a preparation method for an environment-friendly starch-based binder. According to the method, oxidized starch and modified protein are mixed according to a mass ratio of 2:1 to 5:1; polyvinyl alcohol is added according to a mass ratio of oxidized starch to polyvinyl alcohol of 2:1 to 5:1 and uniformly mixed with oxidized starch and modified protein, and an obtained mixture is subjected to a reaction for 2 to 5 hours at a temperature of 50 to 80 DEG C; first liquid of the binder is prepared; the first liquid of the binder is taken out, an auxiliary agent is added, and the environment-friendly starch-based binder is prepared by uniformly mixing the first liquid of the binder and the auxiliary agent; the auxiliary agent is borax and/or citric acid, and the amount of the auxiliary agent accounts for 2 to 5% of the amount of dry starch base. The oxidation starch is prepared by using sodium periodate as an oxidizing agent and mixing the oxidizing agent with starch at a temperature of 20 to 50 DEG C; the raw material in the invention, starch, undergoes a chemical reaction with protein and produces firm chemical bonds, which enables a net structure of the binder to be formed; the net structure and the action of cross-linking agents allow the binder to have improved stability and enhanced water resistance; the invention enables cost to be saved and the goal of environmental protection to be realized.

The invention belongs to the field of hydrogenation of acrylonitrile-butadiene rubber latex. The hydrogenation of acrylonitrile-butadiene rubber latex is realized through the technical scheme as follows: the mixture of acrylonitrile-butadiene rubber latex and hydrazine hydrate is heated to 30 to 70 DEG C, and then constant temperature is kept for 2 to 8 h, and in the process with constant temperature, aqueous solution of sodium periodate is dripped into the mixture, the dripping rate is controlled, and the dripping is accomplished within 2 to 6 hours. The method has the advantages of simple process flow, low cost, less pollution, high hydrogenation rate, low cross linking level of the prepared hydrogenated acrylonitrile-butadiene rubber latex, and good processing property.

The invention discloses a biological medical adhesive capable of producing in situ crosslinking with a wound and a preparation method of the adhesive.The adhesive is prepared with the following method: firstly, sodium periodate oxidative sodium carboxymethylcellulose-dopamine is used for preparing dialdehyde sodium carboxymethylcellulose, a product is dissolved in an MES buffering solution after dialysis and drying, then a certain amount of 1(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-H-hydroxysuccinimide are dissolved in the MES buffering solution as well, stirring reaction is performed by 10-40 min, dopamine is added then, stirring reaction is performed by 8-24h under nitrogen protection, and dialysis, lyophilization and vacuum preservation are performed.Before using, hydrogen peroxide or horse radish peroxidase is used for pretreating the wound, then the wound is coated with the lyophilized adhesive after the lyophilized adhesive is dissolved in a PBS buffering solution, and the wound can be bonded within 2-10 min.The biological adhesive has higher bonding strength under a moist environment in a body, the biocompatibility is good, and the adhesive is biodegradable and capable of promoting wound healing.

The present invention relates to preparation method of aflatoxin immuno-affinity column, including steps of: preparation of hydrazine antibody: employing sodium periodate oxidating unconjugated active area Fc hydroxoy of antibody to aldehyde group, and reacting with adipic dihydrazide to gain hydrazine antibody; preparation of aldehyde solid substrate: oxidating solid substrate containing continued diol structure by sodium periodate to gain aldehyde solid substrate; coupling of antibody and solid substrate: mixing hydrazine antibody and aldehyde solid substrate, adding sodium borohydride to seal unreacted aldehyde group on solid substrate after oscillating reaction to gain coupling antibody solid substrate; column packed. The present invention couples the unconjugated active area Fc end of antibody to solid substrate to remain activity of antibody better; hydrazide derived on antibody instead of solid substrate to avoid nonspecific adsorption of hydrazide group; the combined affinity column not introducing new groups ( such as carboxyl, amido and so on) except coupling antibody to avoid nonspecific adsorption from them.

The invention discloses a preparation method of a cellulose nanocrystal and chitosan composite membrane, and belongs to the field of modification of natural polymers. The method comprises the steps that cellulose nanocrystal (CNC) sol is prepared by conducting concentrated sulfuric acid hydrolysis on microcrystalline cellulose; the cellulose nanocrystal (CNC) sol is reacted with sodium periodate to prepare dialdehyde cellulose nanocrystal (DAC) sol and the cellulose nanocrystal (CNC) sol is reacted with a Girard's reagent T to prepare cationic cellulose nanocrystal (CDAC) sol. The CDAC sol is directly mixed with a chitosan-acetic acid solution, vacuum defoamation tape casting is conducted in a polytetrafluoroethylene mold, drying at room temperature is conducted, and the membrane is obtained. Compared with a pure chitosan membrane, the mechanical property of the prepared cellulose nanocrystal and chitosan composite membrane can be obviously improved, and the swelling degree in water is obviously reduced.

The invention relates to a method for preparing nano-silver by taking modified chitosan as a reducing agent. The method includes: dissolving chitosan in a 3% acetic acid solution with the bath ratio of 1:50, performing ultrasound treatment for 1-12h, then adding 2-10g/L of sodium periodate, performing photophobic reaction at the temperature of 40-80 DEG C for 10-60min, repeatedly cleaning with deionized water, and performing centrifuging and drying for standby application; then mixing chitosan liquid modified by 0.01-100g/L of sodium periodate with 0.01-100g/L of Tollens' reagent, performing ultrasound treatment at the temperature of 20-80 DEG C for 1-10h to obtain nano-silver colloidal solution, repeatedly cleaning with ethyl alcohol and deionized water, and performing centrifuging and drying treatment to obtain nano-silver. By taking chitosan modified with the sodium periodate as the reducing agent and a stabilizer, the method has the advantages of simple technology, mild reaction, greenness, environment friendliness and the like.

The invention relates to biological antibiotic cotton fibers or cotton cloth and a method for preparing the same. In the preparation method, a biological preservative of epsilon-polylysine serving as a bacteriostatic agent is subjected to grafting reaction with cotton fibers or cotton cloth undergoing oxidation treatment by sodium periodate or subjected to adsorption combination with cotton fibers or cotton cloth not undergoing oxidation treatment to form the cotton fibers or cotton cloth having antibiotic and bacteriostatic effects. Because the anti-bacterial agent used in the cotton fibers or cotton cloth is the bacteriostatic agent of epsilon-polylysine, the biological antibiotic cotton fibers or cotton cloth is high in safety. The biological antibiotic cotton fibers or cotton cloth can be used in production of garments and hygienic products.

The invention relates to a diagnostic reagent kit for testing the hepatitis c virus (HCV) and the preparation and test method, which is to add the HCV recombinant antigen used for peridium into the buffer solution, blend it, move into the luminous microplate, make incubation for 18 hours under 4DEG.C, wash the luminous microplate, add into the confining liquid, leave the liquid after incubation and fully dry the luminous microplate to complete the preparation of the pre-peridium luminous microplate; combine the anti-human IgG used for marking and the horse radish peroxidase by improving the sodium periodate to complete the preparation of the enzyme marker; prepare the chemical luminous substrate solution A with luminal, Tween20 and luminous intensifier and prepare the chemical luminous substrate solution B with the hydrogen peroxide. The reagent kit also comprises the sample diluent and concentrated scrub solution. The negative corresponds to the normal human serum while the positive corresponds to the people with serum of pooled serum with HCV antibody. The reagent kit provided in the invention has much higher detection sensitivity than the ELISA, which is safe and reliable, easy to operate with low cost, and without any expensive full-automatic chemical luminous measuring apparatus required.

The invention discloses chitosan-based self-healing gel and a preparation method of the chitosan-based self-healing gel. The preparation method comprises the following steps: putting chitosan in a mixed solvent of KOH, LiOH, NaOH, urea and thiourea, thus obtaining a chitosan solution through a freezing-defreezing process; adding acrylamide in the chitosan solution, thus preparing acrylamide chitosan; oxidizing by sodium periodate, thus preparing oxidized natural polysaccharide; mixing the acrylamide chitosan with the oxidized natural polysaccharide according to a certain proportion, thus preparing a gel material, wherein self repairing on damage after gel is damaged can be realized. According to the preparation method disclosed by the invention, the chitosan gel with good self-healing performance, good biocompatibility and good biodegradability can be obtained, and a wide application prospect in many fields of biomedical materials, tissue engineering, medical packaging materials and the like is obtained.

The invention discloses modified nanocellulose as well as a preparation method and an application thereof. The preparation method of the modified nanocellulose comprises the steps as follows: (1) sodium periodate is used for oxidizing nanocellulose and aldehyde nanocellulose is obtained; (2) aldehyde nanocellulose reacts with polyethylenimine and sodium borohydride sequentially, and the modified nanocellulose is prepared. The prepared nanocellulose has good dispersion performance and high-temperature stability, can improve the rigidity of polymer molecules and form good polymer intermolecularnetwork structures under the higher interaction between the modified nanocellulose and polymers and produce excellent temperature resistance, salt resistance, aging resistance and the like. The prepared modified nanocellulose has a certain oil washing effect.