Aspergillus flavus toxin immuno-affinity column preparation method

A technology of aflatoxin and immunoaffinity, which is applied in the field of preparation of immunoaffinity columns, can solve the problems of increasing non-specific adsorption, cyanogen bromide is highly toxic, and cross-linked antibodies are uneconomical, so as to avoid non-specific adsorption, good active effect
CN101241128AInactive Publication Date: 2008-08-13SHANGHAI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI UNIV
Publication Date
2008-08-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to preparation method of aflatoxin immuno-affinity column, including steps of: preparation of hydrazine antibody: employing sodium periodate oxidating unconjugated active area Fc hydroxoy of antibody to aldehyde group, and reacting with adipic dihydrazide to gain hydrazine antibody; preparation of aldehyde solid substrate: oxidating solid substrate containing continued diol structure by sodium periodate to gain aldehyde solid substrate; coupling of antibody and solid substrate: mixing hydrazine antibody and aldehyde solid substrate, adding sodium borohydride to seal unreacted aldehyde group on solid substrate after oscillating reaction to gain coupling antibody solid substrate; column packed. The present invention couples the unconjugated active area Fc end of antibody to solid substrate to remain activity of antibody better; hydrazide derived on antibody instead of solid substrate to avoid nonspecific adsorption of hydrazide group; the combined affinity column not introducing new groups ( such as carboxyl, amido and so on) except coupling antibody to avoid nonspecific adsorption from them.
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Description

Technical field:

[0001] The invention relates to a preparation method of an immunoaffinity column, in particular to a preparation method of an aflatoxin immunoaffinity column. Background technique:

[0002] Aflatoxins (AFT) are secondary metabolic toxic products of a group of fungi. AFT pollution is serious all over the world. In 1993, AFT was classified as a Class I carcinogen by the Cancer Research Institute of the World Health Organization (WHO). Aflatoxin B in naturally contaminated food and feed 1 (AFB 1 ) is the most common and is the main component of the toxin produced by Aspergillus flavus. It is also the most toxic and carcinogenic, which not only brings significant economic losses to the society, but also seriously threatens the health of consumers. All countries and regions in the world have established strict AFT limit standards, and the limit requirements are becoming increasingly stringent. At present, the detection methods of aflatoxin include thin layer...

Claims

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