2results about How to "Avoid non-specific adsorption" patented technology

The invention discloses a method for preparing a biotin-labeled egg allergen F1 reagent, which comprises the following steps of: a) pretreating an egg to obtain clear egg white liquid; b) mixing 0.2 M NaHCO3 buffer solutions with the same volume as the egg white and the pH value of 9.5, centrifuging to take supernate, dialyzing the supernate at the temperature of 4 DEG C, and then centrifuging to obtain an egg white extracting solution; c) performing buffer solution replacement and filtration operation on the egg white extracting solution to obtain filtrate; d) adding a labeling reagent into the filtrate for labeling to obtain a labeled reagent; e) washing and centrifuging the marked reagent for multiple times to obtain an F1 reagent; f) adding the reagent F1 into glycerol, and then sub-packaging and storing; wherein after the egg white extracting solution is obtained through dialysis in the step b), the pH value of the egg white extracting solution is adjusted to be about 4.0, then the egg white extracting solution is heated for 15-30 min at the temperature of 60-65 DEG C, the egg white extracting solution is rapidly cooled and then centrifuged for 10 min at the temperature of 4 DEG C and the weight of 12000 g, generated precipitates are discarded, supernatant is collected, and the egg white extracting solution which is further purified is obtained.

The application relates to the technical field of biological medicine manufacturing, and discloses a controlled-release type preparation composition for realizing ovary function recovery, which comprises a core active unit, a moisturizing slow-release unit and a plant extract component. The core active unit is an anisotropic core-shell structure microcapsule with asymmetric charge distribution, which is composed of chitosan, polyglutamic acid and an internal compound, and a layer of outwardly radiating carboxyl brush-shaped molecular chain is distributed on the surface of the core active unit. The moisturizing slow-release unit comprises a polymer skeleton formed by rosmarinic acid and hyaluronic acid, and the polymer skeleton internally occludes elemene. The application utilizes a kinetic restriction mechanism to construct an anisotropic surface layer topology, avoids mucus protein adsorption, induces anti-inflammatory immune regulation by physically adapting mucosal receptors, the moisturizing slow-release unit firstly constructs a physical barrier to repair damage, and the core active unit is used to realize component graded controlled release.