73results about How to "Avoid non-specific adsorption" patented technology

The present invention relates to preparation method of aflatoxin immuno-affinity column, including steps of: preparation of hydrazine antibody: employing sodium periodate oxidating unconjugated active area Fc hydroxoy of antibody to aldehyde group, and reacting with adipic dihydrazide to gain hydrazine antibody; preparation of aldehyde solid substrate: oxidating solid substrate containing continued diol structure by sodium periodate to gain aldehyde solid substrate; coupling of antibody and solid substrate: mixing hydrazine antibody and aldehyde solid substrate, adding sodium borohydride to seal unreacted aldehyde group on solid substrate after oscillating reaction to gain coupling antibody solid substrate; column packed. The present invention couples the unconjugated active area Fc end of antibody to solid substrate to remain activity of antibody better; hydrazide derived on antibody instead of solid substrate to avoid nonspecific adsorption of hydrazide group; the combined affinity column not introducing new groups ( such as carboxyl, amido and so on) except coupling antibody to avoid nonspecific adsorption from them.

The invention relates to a water-soluble polymer adsorption material of a coupling cyclodextrin in the field of biomedical materials and an application thereof. The adsorption material comprises a coupling ligand which takes natural polysaccharide with amino or hydroxyl and derivatives thereof or chemosynthesis polymer as carriers and is characterized in that the molecule of the polymer adsorption material has the structure of formula (I). In the formula, R is chosen from natural polysaccharide with amino or hydroxyl and derivatives thereof or chemosynthesis polymer with the viscosity average molecular weight between 6KD and 500KD; X is chosen from C0-C6 alkane chain with O atom, N atom or OH group; CD is chosen from Beta-cyclodextrin or substitutional Beta-cyclodextrin. The adsorption material dissolved into a medical dialyzate can be applied to blood purification treatment for grave hepatitis patients. The adsorption material of the invention has the advantages of removing small molecular protein bound toxin and water soluble toxin molecular at the same time, low treatment cost which is only about one tenth of that of a molecular adsorption recycling system and low non-specificity adsorption of plasma protein.

The invention relates to a water soluble cationic polymer adsorption material in the biomedical material field and the application thereof, which contains the quaterisation reaction synthesis of natural polysaccharides with amino groups and hydroxyls and the derivatives thereof or chemosynthetic polymers. The invention is characterized in that the molecule of the polymer adsorption material has a structure in formula I: in the formula, R is selected from the natural polysaccharides with amino groups and hydroxyls and the derivatives thereof or the chemosynthetic polymers, the molecular weight of which is from 6 KD to 500 KD; X is selected from a C0 to C6 paraffinic chain with O atoms, N atoms or OH groups; R1, R2 and R3 are selected from a C1 to C4 alkyl group. After the adsorption material is dissolved in a medical dialysis solution, the material can be applied to a blood purification treatment for severe hepatitis patients. The invention has the advantages of removing protein-bound toxin and water soluble toxin molecule at the same time, low cost which is only about one tenth of that of a molecular adsorbent recirculating system and little nonspecific adsorption to plasma protein.

The invention discloses an electrochemiluminescence immunosensor based on CdZnTeS quantum dots. The electrochemiluminescence immunosensor comprises CdZnTeS-Ab2, Fe3O4@SiO2-Ab1 with closed non-specificbinding sites, and antigens respectively connected with the CdZnTeS-Ab2 and the Fe3O4@SiO2-Ab1 by mutual action between antigens and antibodies; the CdZnTeS-Ab2 is secondary antibodies marked by CdZnTeS quantum dots; the Fe3O4@SiO2-Ab1 is Fe3O4@SiO2 with the surface being modified with primary antibodies. The invention also provides a preparation method and application of the electrochemiluminescence immunosensor. The electrochemiluminescence immunosensor, the preparation method and the application disclosed by the invention have the beneficial effects that the electrochemiluminescence immunosensor is established on the basis of mutual action between the antigens and the antibodies, so that the specificity of detection is greatly improved, the detection speed is fast, the sensitivity is high, the specificity is good, the detection limit is low, the detectable linear range is wide, the operation is simple and visual detection can be realized.

The invention discloses a liver-targeted platinum-loaded nanometer prodrug for treating liver cancers and a preparation method thereof. The liver-targeted platinum-loaded nanometer prodrug is prepared by taking sodium alginate with the good biocompatibility as a framework to be modified with glycyrrhetinic acid (GA) with a liver cancer cell targeting capacity and a tetravalent platinum compound (PtCl2(OH)2(NH3)2) through a self-assembly technique. In-vitro drug releasing and cytotoxicity tests prove that the platinum-loaded drug nanoparticles have the good blood stability and the intracellular slow-release effect and also have the tumor cell killing capacity. The nanometer prodrug is expected to be used for targeting treatment on the liver cancers and has a good application prospect in the biomedical field.

The invention relates to the technical field of immunology detection, and particularly relates to a kit for quantitative detection of an anti-Jo-1 antibody IgG by using magnetic particle chemiluminescence, and a preparation method and a detection method thereof. The kit for quantitative detection of the anti-Jo-1 antibody IgG by using magnetic particle chemiluminescence includes an Anti-Jo-1 IgG calibrator, an Anti-Jo-1 IgG reagent No. 1, an Anti-Jo-1 IgG reagent No. 2, an Anti-Jo-1 IgG magnetic separation reagent, an Anti-Jo-1 IgG control material and a cleaning solution. The invention also discloses a preparation method and a detection method of the above kit. Based on the traditional film strip immunoassay and enzyme-linked immunosorbent assay, the detection method increases the sensitivity and linearity range by 3-5 orders of magnitude, achieves the quantitative detection, and has the advantages of high sensitivity and specificity, good accuracy, low cost, simple operation and the objective judgment of results. The method cooperates with an automated chemiluminescence immunoassay analyzer to reach complete automation usage, and has broad application prospects.

The invention discloses a preparation method and application of fusion protein with broad spectrum adsorption capacity to antibodies. The method includes the steps: obtaining a protein A and a protein G immune globulin binding zone gene by means of T vector connection and double digestion by the genetic engineering means and via PCR (polymerase chain reaction) amplification; then connecting onto an expression vector pET-23a to form a recombinant plasmid, converting E.coli BL21(DE3), and obtaining a great number of thalli containing the fusion protein AG after inducible expression; and finally, performing ultrasonication, high-temperature heating, precipitation of DNA (deoxyribonucleic acid) and purification of weak anion exchange chromatography and affinity chromatography so that the fusion protein AG is obtained. The fusion protein has dual advantages of the protein A and the protein B and is wider in spectrum combination and low in nonspecific adsorption of non-immune globulin substances of albumin in serum and the like. The fusion protein serving as a ligand is connected to a solid-phase vector matrix to be prepared into adsorbent so that the shortcoming of poor IgG3 binding force of protein A adsorbent is overcome, and can be applied to the fields of antibody purification, blood purification and the like.

The invention relates to the technical field of immunological detection and concretely relates to a kit for magnetic particle chemiluminescent quantitative detection of an anti-SS-B antibody IgG and its preparation method and detection method. The kit for magnetic particle chemiluminescent quantitative detection of an anti-SS-B antibody IgG comprises an Anti-SS-B IgG calibration material, an anti-SS-B IgG regent 1, an anti-SS-B IgG regent 2, an anti-SS-B IgG magnetic separation regent, an anti-SS-B IgG quality control material and a cleaning liquid. The invention also discloses a preparation method and detection method of the kit. Based on the traditional membrane stripe immunization method and enzyme-linked immunosorbent assay, the detection method improves sensitivity and a linear range by 3-5 orders of magnitudes, realizes quantitative determination, has the advantages of high sensitivity, good specificity, good accuracy, low cost, simple operation and objective result determination, realizes full automation by a full-automatic chemiluminescence immunity analyzer and has a wide application prospect.

The invention discloses an ELISA detection method based on nano-enzyme with catalase activity. The ELISA detection method is applicable to a double antibody sandwich method or a dual antigen sandwich method. The method comprises the following steps: firstly modifying detection antibody / detection antigen on a gold nanoparticle; performing ELISA detection and forming a compound of capturing antibody / capturing antigen, to-be-detected antibody / to-be-detected antigen, detection antibody / detection antigen and gold nanoparticle; performing silver platinum dyeing and wrapping the gold nanoparticle surface with silver shell layer and platinum shell layer, thereby acquiring the nano-enzyme Au@AgPt grain with catalase activity; and utilizing Au@AgPt grain to catalyze hydrogen peroxide decomposition for generating hydrogen in an airtight system, increasing the air pressure in the airtight system, detecting the air pressure change, namely, detecting and acquiring the volume of the to-be-detected antibody / to-be-detected antigen. A method of forming enzyme after modifying is applied to the ELISA detection method, so that the ELISA detection method has the advantages of simplicity and quickness, high stability, capability of effectively preventing the nano-enzyme particle catalytic activity caused by nonspecific adsorption and modification from decreasing and capability of supplying new platform to the environment monitoring and disease diagnosis.

The invention discloses a polymer, which has a narrow molecular weight distribution, which is less than 1.3, and has a general formula of B-z-H, P-B-H and P-H-B. In the general formula, B represents a block for bonding a drug, H represents a hydrophilic block, P represents a PEG block, and z represents having or not having environment-responsive chemical bond connection. The polymer forms a micelle with small particle size in water, the micelle can spontaneously aggregate to form an aggregation having a large particle size, and the aggregation has superior tumor specific enrichment than an ordinary drug carrier. The polymer has long body circulation time, and has the feature of being enriched on a tumor. The polymer can carry a drug through a covalent bond and / or a non-covalent bond, and the obtained product can further couple to a targeting molecule and / or a labelled molecule for preparing an administration system or a detection reagent.

The object of the present invention is to provide affinity particles, which are characterized in that: the surface of the organic particles has a phosphorylcholine group represented by the following formula (1) in the form of covalent bonding or adsorption. A ligand with specific affinity for a target substance. Another object of the present invention is to provide an affinity separation method that can easily and accurately separate a target substance by using affinity particles of organic particles.

The invention discloses magnetic red blood cell clusters for enriching circulating tumor cells based on a magnetic separation method, and belongs to the technical field of biological medicines. Red blood cells are combined with nanometer-grade magnetic microspheres to obtain a red blood cell cluster type biomimetic material, and polybrene is modified on the surfaces of the red blood cells, so thatelectrostatic repulsion originally existing between the red blood cells can be eliminated, and further, the red blood cells can be tightly covered on the surfaces of the microspheres in the total layer. The red blood cells pack the nanometer-grade magnetic microspheres to prevent non-specific adsorption of white blood cells on the surfaces of the microspheres, and besides, folic acid (FA) modified on the surfaces of the red blood cell clusters or an antibody specially recognized or combined with a CTCs surface biomarker can realize direct targeting on CTCs, and finally, in a magnetic separating manner, the white blood cells in blood samples of a clinical patient can be removed, so that high-purity CTCs can be efficiently obtained.

The invention provides a polymer material, which is represented by the formula I and contains cholic acid, and a preparation method thereof. The invention further provides a liposome, which takes phosphatide and cholesterol as the carriers and is modified by the polymer material represented by the formula I. Drugs are coated in the phosphatide bi-molecule layer. The provided liposome has double functions of active targeting and passive targeting; and furthermore, the preparation method is simple and practical and can be applied to massive production. The in-vivo experiment results show that the liposome has a strong targeting performance on livers and thus has a good application prospect.

The invention discloses a PEG modified PHPMA material and a preparation method thereof. The PEG modified PHPMA material which is provided by the invention is N-(2'-hydroxyl) propyl methyl acrylamide (HPMA) and / or a copolymer of the derivative of the N-(2'-hydroxyl) propyl methyl acrylamide and polyethylene glycol (PEG). The PEG modified PHPMA material of the invention is a polymer which is formed by the copolymerization of the polyethylene glycol (PEG), poly-N-(2'-hydroxyl) propyl methyl acrylamide (PHPMA) and the derivative of the PHPMA, the copolymer can be obtained by adopting the reversible addition-fragmentation chain transfer (RAFT), the composition and the molecular weight are controllable, and the molecular weight distribution is narrow (the molecular weight distribution d is less than 1, 4). Such polymers can be applied in the targeting drug delivery, which can not only have high targeting property and good biocompatibility, but can also have the function of preventing the protein non-specific absorption after the PEG modification, so the PEG modified PHPMA material is a good drug targeting carrier with the good performance, in particular to the drug targeting carrier of an anti-tumor drug.

The invention discloses an ELISA test method on the basis of nanometer enzyme with peroxidase activity. The ELISA test method is fit for a double antibody sandwich method or a double antigen sandwich method. The ELISA test method comprises the following steps: modifying gold nanoparticle with detection antibody / detection antigen; performing ELISA detection to form the compound of capture antibody / capture antigen, to-be-detected antigen / to-be-detected antibody, detection antibody / detection antigen and gold nanoparticle; performing silver platinum dyeing and wrapping a silver shell layer and a platinum shell layer on the surface of the gold nanoparticle, thereby acquiring the nanometer enzyme Au@AgPt particle with peroxidase activity; utilizing the nanometer enzyme Au@AgPt particle to catalyze a peroxidase enzyme substrate to acquire the colored product so as to detect the quantity of the to-be-detected antigen / to-be-detected antibody. The method of forming enzyme after modifying is adopted, so that the method has the advantages of simplicity, quickness, convenience and high stability; the reduction of the catalytic activity of nanometer enzyme particle caused by nonspecific adsorption and modification can be effectively avoided; and therefore, a new platform is supplied for environment monitoring and disease diagnosis.

The invention relates to the technical field of immunological detection, specifically relates to a kit for quantitatively detecting anti-SS-A antibody IgG by utilizing magnetic particle chemiluminescence, a preparation method and a detection method thereof. The kit for quantitatively detecting anti-SS-A antibody IgG by utilizing magnetic particle chemiluminescence comprises an Anti-SS-A IgG calibration product, an Anti-SS-A IgG reagent 1#, an Anti-SS-A IgG reagent 2#, an Anti-SS-A IgG magnetic separation reagent, an Anti-SS-A IgG quality control material and a cleaning fluid. The invention also discloses the preparation method and the detection method for the kit. According to the detection method, on the basis of the traditional film strip immunization and enzyme linked immunosorbent assay, the sensitivity and the linearity range are increased by 3-5 orders of magnitudes and the quantitative detection is realized; the kit has the advantages of high sensitiveness, strong specificity, high accuracy, low cost, convenience in operation and objective judging result; the full-automatic chemiluminiscence immune analyzer is used for realizing the full-automatic use and has wide application prospect.

The invention discloses an erythrocyte bionic coating for enriching circulating tumor cells. The red blood cells are densely arranged on a substrate, non-specific adsorption of leukocytes on the surface of the substrate is avoided. Meanwhile, the surfaces of the red blood cells are modified with folic acid (FA) or antibodies which can be directly targeted to CTCs. The functionalized red blood cellbionic coating can capture circulating tumor cells more firmly and efficiently by means of the cell membrane surface with fluidity, the highest capture efficiency reaches 90% or above, and the CTCs capture purity reaches 80% or above. The red blood cells are derived from the same donor, foreign matter introduction is avoided, and high-purity CTCs can be obtained through separation by means of a red blood cell lysate.

The invention discloses an erythrocyte cluster for enriching circulating tumor cells (CTCs) based on a size filtering method, and belongs to the technical field of biological medicine. By designing amicron-level CTCs targeting biomimetic material, erythrocytes are combined with a micro-sphere to obtain an erythrocyte cluster type biomimetic material; the surface of each erythrocyte is modified with polybrene, thereby eliminating original electrostatic repulsion between the erythrocytes, and then, dense full-layer coverage of the erythrocytes on the surface of the micro-sphere can be realized.Wrapping the micro-sphere with the erythrocytes can avoid non-specific adsorption of leukocytes on the surface of the microsphere; meanwhile, folic acid (FA) modifying the surface of erythrocyte cluster or an antibody which can specifically recognize and bind surface biomarkers of the CTCs can directly target the CTCs to achieve increase of sizes of the CTCs, and accordingly, leukocytes in a blood sample of a clinical patient can be finally removed in a manner of filtering, and the high-purity CTCs are obtained efficiently.

The invention discloses an immunosorbent for removing inflammatory factors in blood and a preparation method thereof. Based on a variety of intermolecular interactions between the antigenic epitope ofinflammatory factors such as IL-6, TNF-alpha, IL-1 beta and IL-8 and corresponding receptors thereof, affinity ligand of small molecule polypeptide with 5-18 selective amino acids is designed througha computer-assisted drug design method. Specifically, with vinyl acetate-triallyl isocyanurate as a basic skeleton, a mixture of ethyl acetate and alkane as a pore-forming agent, and a synthetic resin with benzoyl peroxide as an initiator as a carrier, alcoholysis activation is carried out, and then the small molecule polypeptide is grafted to obtain the small-molecule ligand immunosorbent whichdoes not adsorb macromolecular proteins and the like. The immunosorbent is simple to prepare, has high adsorbing capacity, high selectivity, good biological and blood compatibility, and relatively lowcost, and provides a new therapeutic method for removing excessive pathogenic inflammatory factors in patients.

The invention discloses a blood platelet magnetizing and immunolabeling analysis method. The method comprises the following steps: uniformly mixing blood platelets and magnetic beads; magnetizing the blood platelets to obtain suspension; uniformly mixing, incubating and washing the magnetized blood platelets and a detected sample; adding a labeled second antibody and incubating to obtain mixed liquid; applying magnetic force to layer the mixed liquid; determining upper reaction liquid or determining after washing the magnetized blood platelets in the lower layer to obtain a result, namely performing double-phase complementary immune analysis. In the mode, the blood platelet magnetizing and immunolabeling analysis method provided by the invention is used for detecting blood platelet related antigen antibody and cross-matching of blood; the magnetic force is applied so that the magnetized blood platelet is washed without centrifuging; the method is easy and convenient to operate; by a labeled antibody adsorption test for directly determining the upper reaction liquid, the non-specific adsorption in an immunolabeling technology is overcome, the processes of closing and washing are reduced, and the time consumption is short. The method can detect a large number of samples and facilitates automation; the two detection results of the double phases can be verified mutually, so that the accuracy of the detection results is improved.

The invention relates to the technical field of immunological detection, in particular to a kit for quantitative detection of a Scl-70 resisting antibody IgG through chemiluminiscence of magnetic particles and a preparing method and detection method of the kit. The kit for quantitative detection of the Scl-70 resisting antibody IgG through chemiluminiscence of the magnetic particles comprises an Anti-Scl-70 IgG calibration sample, a No.1 Anti-Scl-70 IgG reagent, a No.2 Anti-Scl-70 IgG reagent, an Anti-Scl-70 IgG magnetic separation reagent, an Anti-Scl-70 IgG quality control sample and cleaning liquid. The preparing method and detection method of the kit are further disclosed. According to the detection method, based on traditional film strip immunization and an enzyme linked immunosorbent assay adsorption method, sensitivity is improved by 3-5 orders of magnitudes, the linear range is widened by 3-5 orders of magnitudes, and quantitative detection is achieved; the kit has the advantages of being high in sensitivity and specificity, good in accuracy, low in cost, easy and convenient to operate and objective in result judgment, is capable of achieving full-automatic use with the cooperation of a full-automatic chemiluminiscence immune analysis meter, and has wide application prospects.

The present invention is affinity particles that are characterized by having phosphorylcholine groups represented by the following formula (1) covalently bonded onto the surface of inorganic powder and also by having ligands having specific affinity with a certain target substance covalently bonded or adsorbed onto the surface of inorganic powder. The object of the present invention is to provide an affinity separation method that uses affinity particles utilizing inexpensive inorganic particles and is capable of separating the target substance easily and with high accuracy.

The invention belongs to the technical field of immunological detection, and more specifically relates to a kit used for quantitative determination anti-nucleosome antibody Ig G via magnetic micro particle chemiluminiscence, and a preparation method and a detection method thereof. The kit comprises an anti-nucleosome antibody Ig G calibration material, an anti-nucleosome antibody Ig G reagent 1, an anti-nucleosome antibody Ig G reagent 2, an anti-nucleosome antibody Ig G magnetic separation reagent, an anti-nucleosome antibody Ig G quality control product, and a cleaning solution. The invention also discloses the preparation method and the detection method of the kit. The detection method is invented based on conventional membrane strip immunization and enzyme linked immunosorbent assay, sensitivity and linearity range are increased 10<3> to 10<5>, quantitative determination is realized, sensitivity, specificity, and accuracy are high, cost is low, operation is simple and convenient, result judgment is objective, fully automatic application is realized via combination with an automatic chemicaluminescence immunity analyzer, and application prospect is promising.

The invention relates to the technical field of immunology detection, and particularly relates to a kit for quantitative detection of an anti-Sm antibody IgG by using magnetic particle chemiluminescence, and a preparation method and a detection method thereof. The kit for quantitative detection of the anti-Sm antibody IgG by using magnetic particle chemiluminescence includes an Anti-Sm IgG calibrator, an Anti-Sm IgG reagent No. 1, an Anti-Sm IgG reagent No. 2, an Anti-Sm IgG magnetic separation reagent, an Anti-Sm IgG control material and a cleaning solution. The invention also discloses a preparation method and a detection method of the above kit. Based on the traditional film strip immunoassay and enzyme-linked immunosorbent assay, the detection method increases the sensitivity and linearity range by 3-5 orders of magnitude, achieves the quantitative detection, and has the advantages of high sensitivity and specificity, good accuracy, low cost, simple operation and the objective judgment of results. The method cooperates with an automated chemiluminescence immunoassay analyzer to reach fully automation, and has broad application prospects.

The invention belongs to the technical field of blood sugar monitoring products, and particularly discloses a blood sugar monitoring device based on a biocompatibility electrode. The blood sugar monitoring device comprises a continuous monitoring blood sugar monitor body and a probing electrode connected to the continuous monitoring blood sugar monitor, and the surface of the probing electrode is coated with a biocompatibility electrode coating. The blood sugar monitoring device based on the biocompatibility electrode can effectively inhabit the generation of inflammation, improve the blood sugar monitoring precision, and avoid the pain and inconvenience brought to a user due to frequent calibration at the same time.

The invention discloses a novel silica gel matrix surface modification method and application of a surface-modified silica gel matrix. The novel silica gel matrix surface modification method is basedon a five-membered heterocyclic silylating reagent which has an unstable heterocyclic structure, and hydrolysis ring-opening is performed on the reagent and silanol groups, wherein the reactivity is high, and the bonding density is improved significantly; in addition, hydrophilic groups are formed by ring-opened heteroatoms, so that non-specific adsorption of analytes by unreacted silanol groups is avoided effectively. Compared with conventional chlorosilane modification, the novel silica gel matrix surface modification method has high bonding efficiency, mild reaction conditions and no cross-linking, the pore structure of silica gel can be ensured, and therefore the separation efficiency can be improved effectively by a chromatographic stationary phase prepared from the silica gel matrix;and the chromatographic stationary phase can be widely applied to separation of alkaline analytes and biological samples.

The invention belongs to the technical field of medicines for treating tumors, and provides a nano-particle for improving tumor tissue penetrability. The nano-particle comprises hollow mesoporous Prussian blue nano-particles, phase-change materials, tumor matrix degradation materials and anti-tumor medicines, the phase-change materials are loaded in the hollow portions of the hollow mesoporous Prussian blue nano-particles, the tumor matrix degradation materials and the anti-tumor medicines are embedded into the phase-change materials, and the phase-change materials comprise lauric acid, 9-heptadecanone and decanoic acid or 1-tetradecyl alcohol. According to the nano-particle for improving the tumor tissue penetrability, the anti-tumor medicines can enter tumor tissues, and the anti-tumor rate of the nano-particle can reach 81.3%.

The invention provides a high-polymer material with cholic acid, a method for preparing the high-polymer material and liposome. The high-polymer material is shown as a formula I. Phospholipid and cholesterol are used as carriers for the liposome, the liposome is modified by the high-polymer material shown as the formula I, and medicines are entrapped in bi-molecular layers of the phospholipid. Thehigh-polymer material, the method and the liposome have the advantages that an active targeting effect and a passive targeting effect can be realized by the liposome; the method is simple and practical, amplification production can be facilitated, the high-polymer material and the liposome are high in targeting capacity for livers as shown in in-vivo experiments, and accordingly the high-polymermaterial, the method and the liposome have excellent application prospects.