The invention relates to a room-temperature self-crosslinking wood lacquer emulsion which is characterized by being prepared from the following components: methyl methacrylate, styrene, butyl acrylate, diacetone-acryloamide, glycidyl methacrylate, methacrylic acid, polycarbodiimide resin, adipic dihydrazide, an emulsifier, an initiator, a neutralizer, a molecular weight regulator and deionized water. The preparation method comprises the following steps: adding 10-50% of emulsifier aqueous solution into a reaction kettle, stirring and raising the temperature; in an emulsifying kettle, sequentially adding monomer materials into the residual emulsifier aqueous solution in a high speed disperse state and stirring; then, adding 5% of monomer pre-emulsion into the reaction kettle, adding an initiator solution, and starting to add the residual emulsion and initiator solution dropwise when the temperature of the reaction kettle reaches 80 DEG C, and then starting cooling the reaction kettle; and adding the neutralizer, adipic dihydrazide and polycarbodiimide resin into the reaction kettle, stirring for 30-40 minutes and filtering and packaging. According to the room-temperature self-crosslinking wood lacquer emulsion provided by the invention, cross-linking reaction of diacetone-acryloamide and adipic dihydrazide, polycarbodiimide resin and methacrylic acid is adopted, and the double crosslinking reaction is of great benefit to enhancing the adhesive property of a film.