The invention belongs to the technical field of biological engineering, and particularly relates to an active cell repairing agent and a preparation method thereof. The active cell repairing agent comprises the following components in percentage by mass: 8-13% of aloe polysaccharide, 6-8% of chitosan oligosaccharide, 4-6% of chitosan, 4-5% of hyaluronic acid, 3-5% of vitamin B5, 2.5-5% of collagen, 2-3% of glutathione, 1-3% of apple polyphenol, 4-8% of octadecanol, 2-5% of albolene, 3-5% of glycerin monostearate, 6-10% of glycerinum, 0.2-0.7% of phenoxyethanol and 23.3-54.3% of water. The active cell repairing agent has the technical advantages that the active cell repairing agent is very good in effect in skin repairing for healing skin injury traumas, reducing cicatrization, reducing postoperative adhesions and the like, free of toxic or side effect and relatively great in clinical application value.