6 results about "Dihydrolipoic acid" patented technology

The invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and application of the culture medium. The culture medium contains inorganic salt, amino acid, vitamins, energy substances, a buffer substance, an antioxidant, sterol, polyamine, an additive and an indicator, and the antioxidant comprises dihydrolipoic acid and S-acetyl-L-glutathione. According to the invention, the culture medium for serum-free suspension culture of the BHK-21 cells, which is clear in components and remarkable in culture effect, can be provided. The added dihydrolipoic acid and S-acetyl-L-glutathione are combined as antioxidants of the culture medium, so that the survival rate of BHK-21 cells cultured in the culture medium is improved while the oxidation resistance of the serum-free suspension culture medium is improved.

The present application relates to a kind of hyperbranched polymer, nano preparation and its preparation method and application, belong to functional medical material and nanotechnology field.The hyperbranched polymer of the present application can be broken in ROS environment Release dihydrolipoic acid, play the role of antioxidant, be reduced to lipoic acid in vivo, and then play its role as coenzyme, participate in mitochondrial tricarboxylic acid cycle, restore mitochondrial function, thereby play the role of antioxidant and play the role of treating ischemic stroke.The nano preparation provided by the present application can be quickly distributed to brain in a short time, temporarily increase the paracellular permeability of BBB by inhibiting the expression of multi-drug resistance protein P-gp and ATP-dependent drug efflux protein on BBB, and the process of opening BBB by borneol is physiological and reversible, without destroying the structural integrity of blood-brain barrier.

The invention discloses a silver nanocluster with red emission and detection and antibacterial properties thereof, the silver nanocluster is prepared by adopting dihydrolipoic acid as a ligand and sodium borohydride as a reducing agent through an optimized synthesis process, the quantum yield is as high as 14.8%, the fluorescence lifetime is 7.91 microseconds, and the silver nanocluster has excellent fluorescence characteristics and stability. High-selectivity detection of the heavy metal ion Ni < 2 + > can be realized based on a fluorescence resonance energy transfer mechanism, and the detection limit is as low as 68 nM. Meanwhile, four types of tetracycline antibiotics (TCs) of oxytetracycline (OTC), tetracycline (TC), doxycycline (DOX) and aureomycin (CTC) are recognized through specific ratio fluorescence response. The compound has an efficient inhibition effect on common pathogenic bacteria such as gram-positive bacteria and gram-negative bacteria, and the bacteriostasis rate reaches 80% or above. The hydrogel dressing prepared by compounding AgNCs and a natural polymer material sodium alginate can effectively inhibit wound infection and promote tissue regeneration and wound healing.

The present invention related to a compound for use in prevention or treatment of a neurodegenerative disease associated with the formation of stress granules. The compound is selected from lipoic acid, lipoamide, dihydrolipoic acid, and dihydrolipoamide. The invention further relates to a method of identifying a compound that modulates a characteristic associated with one or more condensates comprising a condensate-associated molecule, comprising: (a) contacting the compound with a cellular composition comprising one or more condensates or a cellular composition capable of forming one or more condensates, and (b) determining the characteristic associated with the one or more condensates. A modulation in the characteristic, as compared to a reference, indicates that the compound modulates the characteristic associated with the one or more condensates.

The application belongs to the technical field of biological medicine, and particularly relates to an adrenaline injection and a preparation method thereof. The application provides an adrenaline injection, 1 mg of adrenaline, 9 mg of sodium chloride, 0.02%-0.5% of dihydrolipoic acid, 0.05%-0.5% of disodium edetate, and appropriate hydrochloric acid are contained in each 1 mL of the injection, and the rest is water for injection. The dihydrolipoic acid and the disodium edetate are innovatively added in the prescription as antioxidants, and the antioxidants synergistically play a stabilizing role. The prescription process of the application has good applicability and high stability, the injection can be subjected to terminal sterilization, and the safety and effectiveness of the product in the clinical use can be fully ensured.

This invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and its application. The culture medium contains inorganic salts, amino acids, vitamins, energy substances, buffers, antioxidants, sterols, polyamines, additives, and indicators. The antioxidants include dihydrolipoic acid (DLA) and S-acetyl-L-glutathione. This invention provides a culture medium with clearly defined components and significant culture effects for serum-free suspension culture of BHK-21 cells. The added DLA and S-acetyl-L-glutathione, as a combination of antioxidants in the culture medium, enhance the antioxidant properties of the serum-free suspension medium while also improving the cell viability of BHK-21 cells cultured in the medium.