12 results about "Inner membrane" patented technology

The application provides an engineered probiotic with intracellular chimeric nanoscale enzyme, which is a probiotic modified with several antioxidant enzymes on an inner membrane; the antioxidant enzyme is composed of a metal oxide nanoscale enzyme with a particle size less than 10 nm and a targeting molecular structure and a saccharide molecular structure modified on the surface of the metal oxide nanoscale enzyme; the targeting molecular structure and the saccharide molecular structure are connected to the surface of the metal oxide nanoscale enzyme through a connecting group containing a metal ligand structure based on coordination and electrostatic interaction, and the antioxidant enzyme accounts for 5-20% of the mass of the engineered probiotic. The application also provides a method for preparing the engineered probiotic and application of the engineered probiotic in preparation of a medicine or food for preventing or treating antibiotic-associated diarrhea. The engineered probiotic can realize efficient elimination of hydroxyl radicals near a membrane induced by an antibiotic, so as to accurately inhibit a lipid peroxidation reaction and protect the integrity of a cell membrane.

A fusion protein comprises (1) a first moiety that targets and orients the fusion protein to mitochondria inner membrane and (2) a second moiety that provides light-activated proton pump function when integrate into the mitochondria inner membrane. The fusion protein can be used for modulating hypoxia signaling in a subject, revitalizing cells, modulating T cell function, improving feed efficiency, prolonging lifespan and treating cancer.

The invention belongs to the technical field of drug delivery and tumor immunotherapy, and particularly relates to an oncolytic virus vesicle as well as a preparation method and application thereof. The preparation method comprises the following steps: co-incubating oncolytic adenovirus and platelets in a buffer system containing prostaglandin E2, and inducing to form an inner membrane encapsulation body; and activating and releasing the vesicles under the conditions of 36-38 DEG C and 4-6% CO2, and carrying out gradient centrifugal collection of 300g, 2000g, 10000g and 100000g. The particle size of the oncolytic virus-loaded vesicle is 50-200nm, the surface of the oncolytic virus-loaded vesicle contains CD41, CD61 and P-selectin, and the oncolytic virus-loaded vesicle can realize long circulation and lung targeting delivery of oncolytic viruses, is suitable for preparing drugs for treating breast cancer and breast cancer lung metastasis, and has a remarkable tumor inhibition effect.

The invention discloses an HlyA secretion system for increasing the secretion amount of substrate protein and a construction method and application thereof.The HlyA secretion system comprises a first component, a second component and a third component, the first component comprises an inner membrane transporter HlyB and a membrane interstitial adaptor protein HlyD, and the inner membrane transporter HlyB and the membrane interstitial adaptor protein HlyD are obtained by expressing modified hlyBD operons; according to the modified hlyBD operon, a nucleotide sequence for coding a hexa-polyhistidine tag is inserted behind an hlyD gene initiation codon in the hlyBD operon; and a second component: an outer membrane channel protein TolC derived from a host cell. According to the invention, the HlyD protein is specifically modified, so that the secretion amount of the HlyA secretion system to the substrate protein is obviously increased.

The utility model relates to the technical field of storage water tank, concretely relates to a full-closed storage water tank, including the shell, be provided with soft inner membrane in the shell, be provided with the air pressure balance subassembly of intercommunication with the inner wall of shell on the shell, the top of soft inner membrane is provided with the water inlet pipe of intercommunication with soft inner membrane inside, the bottom of soft inner membrane is provided with the water outlet pipe of intercommunication with soft inner membrane inside. With the help of soft inner membrane to store water, when water, soft inner membrane along with the increase of water, volume becomes big, at the same time with the help of air pressure balance subassembly to discharge the extra air in the shell, when water, soft inner membrane along with the reduction of water, volume becomes small, at the same time with the help of air pressure balance subassembly to fill in the air. The whole water and water outlet process, air only exists between the shell and soft inner membrane, will not enter the soft inner membrane inside and contact with water, like this can effectively avoid the oxidation reaction or the problem of bacteria breeding after air and water contact.

The invention discloses an oxygen enrichment membrane assembly and an oxygen enrichment machine, the oxygen enrichment membrane assembly comprises a shell and a membrane layer, the membrane layer is an oxygen enrichment membrane, a mounting cavity is formed in the shell, the membrane layer is arranged in the mounting cavity of the shell, and the membrane layer is connected with the shell and divides the mounting cavity into a membrane inner cavity and a membrane outer cavity; a first vent hole and a second vent hole which are communicated with the membrane outer cavity are formed in the shell, and an airflow channel flowing through the outer surface of the membrane layer is formed between the first vent hole and the second vent hole, so that nitrogen molecules attached to the outer surface of the membrane layer are discharged out of the membrane outer cavity along the airflow channel; the membrane inner cavity is communicated with a negative pressure device in a sealed mode, and the negative pressure device is used for driving air to enter the membrane inner cavity from the membrane outer cavity through the membrane layer. The airflow channel flowing through the outer surface of the membrane layer is formed between the first vent hole and the second vent hole which are communicated with the outside of the membrane, nitrogen molecules attached to the outer surface of the membrane layer are discharged through airflow of the airflow channel, and the situation that the oxygen separation efficiency is reduced due to the fact that the nitrogen molecules block the membrane layer is avoided.

The application discloses a Parkinson's disease targeted application of a pueraria exosome loaded with a mitochondria-activated miRNA, and belongs to the field of biological applications.The miRNA comprises at least one of miRNAs shown in sequences as shown in SEQ ID No, 1 to SEQ ID No, 23.The pueraria exosome and the miRNA delivered thereby in the application show a good treatment effect on Parkinson's disease, and mainly play a role in improving mitochondrial dysfunction, such as promoting mitochondrial autophagy, improving mitochondrial membrane potential, reducing oxidative stress, maintaining the integrity of the mitochondrial inner membrane respiratory chain, and increasing ATP generation.The application further discloses a delivery scheme of the pueraria-derived exosome as the miRNA carrier by using neuron-targeted modification, which can improve the targeting and stability of the miRNA, and overcome the problems of low miRNA drug delivery efficiency and easy degradation.The application is expected to promote the treatment process of Parkinson's disease through the neuron-targeted pueraria exosome and the miRNA delivered thereby.

The invention discloses hydrogel microspheres for inhibiting mitochondrial DNA release and improving osteoarthritis and application, and aims at the clinical dilemma that early and middle-stage osteoarthritis lacks specific treatment, the invention discovers that mechanical overload induced mitochondrial membrane potential reduction (from-180mV to-100mV) leads to abnormal opening of VDAC, miMOMP change and mtDNA outleakage, and the hydrogel microspheres can inhibit mitochondrial DNA release and improve osteoarthritis. The method is a key mechanism for steady-state imbalance of cartilage cells. On the basis, a Fe / BiOCl (at) AHAMA-GelMA dual response system is constructed, wherein the Fe / BiOCl nanosheet has positive charges (+ 25-30 mV) in a resting state and can target a mitochondrial inner membrane, and local negative potential (about-50 mV) is generated under mechanical stimulation to release electrons to recover membrane potential; the AHAMA-GelMA hydrogel microspheres are combined with amino groups on the surface of cartilage through aldehyde groups to realize spatial positioning, and a targeted mechanical overload area is recognized by utilizing the specificity of MMPs to gelatin. In-vivo and in-vitro experiments prove that the system can convert overload abnormal stress into repair electric energy, inhibit mtDNA outleakage, remarkably improve the mitochondrial function and relieve cartilage degeneration, and a new disease modification treatment strategy is provided for osteoarthritis.

This invention relates to the field of nanomedicine technology and discloses the application of nano-Amy@NPs-MM / PL1 in the treatment of endometriosis. The nano-Amy@NPs-MM / PL1 comprises a nanocarrier PEG-PLGA, a nanomedicine Amy, a nano-coated macrophage membrane, and a targeting peptide PL1. The steps are: S1, macrophage membrane extraction: using a cell membrane extraction kit, macrophage membranes are separated; S2, targeting peptide insertion into the macrophage membrane: the targeting peptide PL1 is inserted into the macrophage membrane to obtain the targeting macrophage membrane MM / PL1; S3, preparation of PEG-PLGA-loaded Amy. This invention demonstrates that Amy@NPs-MM / PL1 inhibits the activation of the TNF-α / TNFR1 / NF-κB signaling pathway, blocks the downstream caspase-1 / GSDMD-mediated pyroptosis cascade, reduces the release of inflammatory factors, and inhibits angiogenesis and the expression of invasive factors. It achieves multi-level intervention from anti-oxidation and anti-pyroptosis to neuroprotection and metabolic regulation, providing a new strategy with both theoretical innovation and clinical translational potential for the comprehensive treatment of endometriosis.

The invention provides a method for activating granulate bodies, which mainly comprises the following steps of: supplying negative charges and active oxygen to the granulate bodies from the outside through a negative charge and active oxygen supply unit so as to serve as raw materials for redox of the granulate bodies; the calcium ion channel promoting unit promotes the calcium ions in the body to enter the matrix space of the granulonema through calcium transport proteins on the granulonema inner membrane, so that the internal flow of the calcium ions can promote the depolarization of the granulonema, and the oxidative phosphorylation activity of the granulonema is improved. Thus, granulocyte activation and ATP production are enhanced.

The invention relates to mitochondrial targeting double-response composite hydrogel and a preparation method thereof. The preparation method comprises the following steps: synthesizing cationic amylose; preparing a compound; preparation of grafted triphenylphosphine and a composite hydrogel; according to the invention, the amylose is grafted with the TPP and protects the FGF21 structure, the amylose not only solves the problem of sudden release of the TPP, but also anchors the BPQDs in a micelle shell layer, so that the photo-thermal effect is closer to mitochondria, and the activity of the FGF21 is obviously protected. In addition, triple effects of photo-thermal, FGF21 signal regulation and mitochondrial targeting are combined for local implantation treatment of endometriosis for the first time, and almost complete fading of deep ectopic lesions can be realized.