55 results about "Membrane channel" patented technology

Nucleic acid vectors encoding light-gated cation-selective membrane channels, in particular channelrhodopsin-2 (Chop2), converted inner retinal neurons to photosensitive cells in photoreceptor-degenerated retina in an animal model. Such treatment restored visual perception and various aspects of vision. A method of restoring light sensitivity to a retina of a subject suffering from vision loss due to photoreceptor degeneration, as in retinitis pigmentosa or macular degeneration, is provided. The method comprises delivering to the subject by intravitreal or subretinal injection, the above nucleic acid vector which comprises an open reading frame encoding a rhodopsin, to which is operatively linked a promoter and transcriptional regulatory sequences, so that the nucleic acid is expressed in inner retinal neurons. These cells, normally light-insensitive, are converted to a light-sensitive state and transmit visual information to the brain, compensating for the loss, and leading to restoration of various visual capabilities.

An array of airfoil-shaped micro-mixers that enhances fluid mixing within permeable membrane channels, such as used in reverse-osmosis filtration units, while minimizing additional pressure drop. The enhanced mixing reduces fouling of the membrane surfaces. The airfoil-shaped micro-mixer can also be coated with or comprised of biofouling-resistant (biocidal / germicidal) ingredients.

Microbial type rhodopsins, such as the light-gated cation-selective membrane channel, channel-rhodopsin-2 (Chop2 / ChR2) or the ion pump halorhodopsin (HaloR) are expressed in retinal ganglion cells upon transduction using recombinant AAV vectors. Selective targeting of these transgenes for expression in discrete subcellular regions or sites is achieved by including a sorting motif in the vector that can target either the central area or surround (off-center) area of these cells. Nucleic acid molecules comprising nucleotide sequences encoding such rhodopsins and sorting motifs and their use in methods of differential expression of the transgene are disclosed. These compositions and methods provide significant improvements for restoring visual perception and various aspects of vision, particular in patients with retinal disease.

The invention discloses a high-efficiency and high-sensitivity biomacromolecule trans-membrane and staining system. The high-efficiency and high-sensitivity biomacromolecule trans-membrane and staining system comprises a working unit and an operating portion which is associated with the working unit, wherein the working unit is provided with at least one channel used for detecting and analyzing a sample, the operating portion is used for creating at least one staining or trans-membrane channel parameter, and each staining or trans-membrane channel parameter comprises at least one electric field cycle. The invention further discloses a device based on the high-efficiency and high-sensitivity biomacromolecule trans-membrane and staining system. The device comprises a base and a shell, an operating panel is arranged on the outside of the shell, a working region, a system power source and a working circuit board are arranged in the base and the shell, the system power source and the working circuit board are connected with the working region, the working region is at least provided with one pull-out-type independent working chamber, and a positive and negative electrode board providing an electric field is installed in the working chamber. According to the system or the device, rapid staining and trans-membrane of biomacromolecules can be controlled by driving movement of biological charged particles through the electric field; meanwhile, the requirement for high sensitivity of biological detection can be further met.

Methods for preparing a polymer membrane on a porous support may include providing a porous support having an outer wall, a first end, a second end, and porous channel surfaces that define a plurality of channels through the porous support from the first end to the second end. The plurality of channels includes membrane channels. The channel surfaces that define the membrane channels are membrane-channel surfaces. The polymer membrane may be coated onto the porous support by first establishing a pressure differential between the outer wall and the plurality of channels. Then, a pre-polymer solution may be applied to the membrane-channel surfaces and, optionally, the first and second ends, by slip coating or emulsion coating while the pressure differential is maintained. This results in formation of a pre-polymer layer on at least the membrane-channel surfaces. Then, the pre-polymer layer may be cured to form the polymer membrane.

The invention provides a recombinant strain of Escherichia coli, a preparation method and application thereof. Specifically, the ompC site of Escherichia coli B strain is transformed, the integrity of the ompC protein is restored, and the expression regulation mechanism of the two membrane channel proteins of ompC and ompF is provided. . The modified new strain can improve the expression and secretion efficiency of the recombinant protein.

The invention discloses a method for detecting endotoxin. The method comprises: incubating a sample to be tested with a fluorescently labeled LPS aptamer; adding reduced graphene oxide and TBE buffer solution, and incubating; and incubating the incubated sample Load the sample into the inlet of the microfluidic chip, apply a voltage between the inlet and outlet of the microfluidic chip for processing, the inlet is the anode, and detect the fluorescence between the Nafion membrane channel and the anode after voltage treatment Intensity; determine the content of LPS in the sample to be tested according to the fluorescence intensity and the standard curve. The method of the invention has a minimum detection limit of 8 fM, a wide linear range, high sensitivity, good selectivity, strong anti-interference ability, and can quickly distinguish Gram-positive bacteria, Gram-negative bacteria and fungi.