55 results about "Membrane channel" patented technology

Nucleic acid vectors encoding light-gated cation-selective membrane channels, in particular channelrhodopsin-2 (Chop2), converted inner retinal neurons to photosensitive cells in photoreceptor-degenerated retina in an animal model. Such treatment restored visual perception and various aspects of vision. A method of restoring light sensitivity to a retina of a subject suffering from vision loss due to photoreceptor degeneration, as in retinitis pigmentosa or macular degeneration, is provided. The method comprises delivering to the subject by intravitreal or subretinal injection, the above nucleic acid vector which comprises an open reading frame encoding a rhodopsin, to which is operatively linked a promoter and transcriptional regulatory sequences, so that the nucleic acid is expressed in inner retinal neurons. These cells, normally light-insensitive, are converted to a light-sensitive state and transmit visual information to the brain, compensating for the loss, and leading to restoration of various visual capabilities.

An array of airfoil-shaped micro-mixers that enhances fluid mixing within permeable membrane channels, such as used in reverse-osmosis filtration units, while minimizing additional pressure drop. The enhanced mixing reduces fouling of the membrane surfaces. The airfoil-shaped micro-mixer can also be coated with or comprised of biofouling-resistant (biocidal/germicidal) ingredients.

The invention relates to an oxidation nanofiltration membrane reactor. The oxidation nanofiltration membrane reactor consists of a reaction-separation system (a raw material pump, an oxidation reaction kettle, a circulating pipe and a gas distribution plate), a pressurizing system (an ozone generator, a buffer tank, a pneumatic booster pump, an air compressor and a pressure-stabilizing tank) and a tail gas treatment system (an ozone absorption device), wherein a membrane component is encapsulated in a membrane component and placed into the reaction kettle; the circulating pipe is arranged at the position of a side arm of the kettle body; and the gas distribution plate is positioned on the top of a settling region. In the reactor, the kettle body serves as a liquid ascending region, the circulating pipe serves as a liquid descending region, gas is redistributed through a membrane channel, and gas liquid phases form slug flow in the membrane channel, so that gas liquid mass transfer is enhanced, the reaction process is enhanced and the utilization ratio of the gas is increased; and high-pressure ozonized air generated by the pressurizing system serves as a reaction source and a circulating power source of the reactor, so that the degradation of organic matters in the reactor is accelerated and a necessary impelling force is provided for separation of the nanofiltration membrane.The reactor has the advantages of simpleness and convenience in operation, high mass transfer efficiency, low energy consumption, easiness in amplification and the like.

Microbial type rhodopsins, such as the light-gated cation-selective membrane channel, channelrhodopsin-2 (Chop2/ChR2) or the ion pump halorhodopsin (HaloR) are expressed in retinal ganglion cells upon transduction using recombinant AAV vectors. Selective targeting of these transgenes for expression in discrete subcellular regions or sites is achieved by including a sorting motif in the vector that can target either the central area or surround (off-center) area of these cells. Nucleic acid molecules comprising nucleotide sequences encoding such rhodopsins and sorting motifs and their use in methods of differential expression of the transgene are disclosed. These compositions and methods provide significant improvements for restoring visual perception and various aspects of vision, particular in patients with retinal disease.

Cyclic homodetic peptides having a repeating D-L-chirality motif are shown to have a stable disk conformation with the amino acid side chains extending radially outward and the carbonyl and amino groups extending axially upward or downward. Such cyclic peptides can be employed as subunits in the assembly of molecular tubes. Cyclic peptides having a repeating D-L-chirality motif and lacking mutually repulsive side-chains are shown to stack atop one another in an anti-parallel fashion and are shown to be held together by the formation of β-sheet hydrogen bonding. The stacked cyclic peptides form a molecular tube having a central channel. The diameter of the channel is determined by the size cyclic peptide. If the cyclic peptide includes ionizable amino acid residues, e.g. glutamic acid or lysine, assembly and disassembly of the molecular tubes can be controlled by varying the pH. If the cyclic peptide includes hydrophobic amino acid residues, the molecular tube will insert into a lipid membrane. In such instances, the molecular tube provides a transmembrane channel. The channel can be gated or ungated. Molecular tubes can be terminated with a terminal cyclic peptide having methylated amino groups in one orientation. Molecular tubes may be employed as drug carriers, molecular sieves, reaction vessels, membrane channels, and other uses.

Methods for preparing a polymer membrane on a porous support may include providing a porous support having an outer wall, a first end, a second end, and porous channel surfaces that define a plurality of channels through the porous support from the first end to the second end. The plurality of channels includes membrane channels. The channel surfaces that define the membrane channels are membrane-channel surfaces. The polymer membrane may be coated onto the porous support by first establishing a pressure differential between the outer wall and the plurality of channels. Then, a pre-polymer solution may be applied to the membrane-channel surfaces and, optionally, the first and second ends, by slip coating or emulsion coating while the pressure differential is maintained. This results in formation of a pre-polymer layer on at least the membrane-channel surfaces. Then, the pre-polymer layer may be cured to form the polymer membrane.

The invention discloses a solid-liquid separation device for organic carbon sources in excess sludge. The solid-liquid separation device comprises an anaerobic hydrolysis reactor, a screw pump, a chemical cleaning tank, a chemical cleaning pump, a ceramic membrane microfiltration device I, a ceramic membrane microfiltration device II and a carbon source storage tank; the bottom of the anaerobic hydrolysis reactor is communicated with the screw pump; the outlet of the screw pump is communicated with the ceramic membrane microfiltration device I and the ceramic membrane microfiltration device II respectively; the chemical cleaning tank is communicated with the chemical cleaning pump; the outlet of the chemical cleaning pump is communicated with the ceramic membrane microfiltration device I and the ceramic membrane microfiltration device II respectively; and both the ceramic membrane microfiltration device I and the ceramic membrane microfiltration device II are communicated with the carbon source storage tank through hydrolyzed filtrate pipelines. Through the solid-liquid separation device, the dissoluble organic carbon sources can be effectively eluted, so that hydrolysis and acidification of the sludge are promoted; membrane channel blockage can be controlled and reduced in double flow directions, and continuous operation is realized; and a high solid-liquid separation effect is achieved, and the concentration of effluent microbes is less than 100 microbes per milliliter.

Embodiments profile cannabinoids using a nanopore detector. Embodiments use an applied potential to electrophoretically draw a nanopore blockade reporter molecule into a nanopore channel to establish an electrophoretic molecular channel-capture for the reporter molecule. The reporter molecule includes one of an aptamer or a monoclonal antibody and has a specific binding to at least one of: a specific cannabinoid, a particular cannabinoid isoform; or a specific cannabinoid family. Embodiments receive a blockade channel current signal in response to a nanoscale membrane channel of the nanopore detector being partially blockaded by a presence of the reporter molecule non-covalently bonded to the membrane channel. Embodiments receive a blockade signal for the reporter molecule that is toggling between more than one level.

The invention provides a dunaliella salina cDNA library induced by high salt as well as screening its cDNA fragment and gene. Wherein, it constructs the library with inhibiting hybridization, and discloses the cDNA fragment and dunaliella salina Na+/H2PO4- co-ttransfer membrane channel protein cDNA, whole gene and its amino acid sequence. This cDNA can screen the specific express of dunaliella salina induced by high salt, and settles foundation for corresponding molecular mechanism research.

The invention provides a 3D printing device which comprises a housing, a crucible arranged in the housing, a cover plate fixed at the top of the housing and sealing the crucible, a liquid transportingdevice and a pressure transporting device communicating with the crucible, an outer spray nozzle fixed at the bottom of the housing, a first temperature control device for controlling the liquid temperature in the crucible, and an industrial control device, and further comprises a second thermocouple and a second heater, which are arranged in the outer spray nozzle and are close to an outlet, anda second temperature controller electrically connected with the second thermocouple and the second heater. A slide block is arranged in the outer spray nozzle, is connected with a first motor, and can open or close the outlet under the driving action of the first motor. The first motor is electrically connected with the industrial control device. A concentrated liquid flow is in the high-temperature state by heating of the heater, so that the condition that liquid is cooled and solidified to block the outlet of the spray nozzle is avoided, and a gas membrane channel constrains the concentrated liquid flow, so that the concentrated liquid flow is prevented from being in contact with the spray nozzle, and the blocking of the spray nozzle is further prevented.

The invention relates to a 3D sea water desalination chip device based on an ion concentration polarization principle. The 3D sea water desalination chip device comprises a base adopting 3D printing and a shell adopting 3D printing, a convexly formed buffer solution channel is formed in the base, and a micro-nano membrane channel, a desalted water channel, a concentrated saline water channel and a water outlet are also formed in the base; after the shell and the base are combined, a saline water channel is formed. After a 3D printing material is adopted for combination, fit is tight, no seam is formed, and leakproofness is extremely good; the base and the shell are clamped, connected and fixed by virtue of a 3D printing surrounding edge, the fixed position is accurate, glue or a nail does not need to be used, the integrity can be guaranteed, and the device can not be destroyed by the glue or nail; the manufacturing cost is low, and the device has mass production potency and is recyclable; the device is supported by 3D printing, so that the precision of the dimensions and shape of the device is not limited by familiarity degree of skills of workers, and the obtained finished product has relatively good dimensions and precision; the ion concentration polarization principle is utilized for carrying out sea water desalination, the energy consumption is low, the desalination efficiency is high, and no harm is done to the environment.

The invention relates to a novel cyclic peptide containing 2-methyl-3-aminobenzoic acid (gamma-Mba). The cyclic peptide contains at least one 2-methyl-3-aminobenzoic acid (gamma-Mba) and 3-31 nonpolar amino acids, wherein the nonpolar amino acids are L- or D- alpha-amino acids and are any one or more of 8 natural nonpolar amino acids. The cyclic peptide can be assembled into nanotubes, so as to be used as medicament carriers, membrane channels, biosensors and the like.

The invention discloses a multi-channel puncture apparatus, which comprises a porous operating platform placed outside the abdominal cavity, a celiac channel tube connected to the porous operating platform and is suitable for insertion into the abdominal cavity. The porous operating platform comprises a bowl-shaped shell with one end closed and the other end open, a platform fixing ring which is arranged at the opening end of the bowl-shaped shell and is suitable for forming a cavity with the bowl-shaped shell, and several separate instrument channels located at the closed end of the bowl-shaped shell and being suitable for communicating with the cavity. The celiac channel tube comprises a flexible membrane channel assembly and a channel fixing ring being suitable for the flexible membranechannel assembly. The channel fixed ring is suitable for coupling with the platform fixing ring which is provided with at least a set of locking components being symmetrically arranged for simultaneously locking the channel fixing ring and the platform fixing ring.

The invention discloses a method for preparing a high performance silicalite-1 zeolite membrane, which relates to a method for preparing a zeolite membrane, wherein a mixed solution which is prepared through porous anodic alumina or silica ceramic tube carriers by utilizing acacia gum, carboxymethyl cellulose, tannic acids, soluble starch, sucrose and glycerol is soaked and dried for many times, deionized water is weighed and is hydrothermally compounded to prepare the silicalite-1 zeolite membrane, the dried silicalite-1 zeolite membrane is activated fat a certain time in 400-600 DEG C to remove a diaphragm agent in a zeolite membrane channel. The method for preparing the high performance silicalite-1 zeolite membrane can effectively improve separation performance, is beneficial to reduce preparation cost of the silicalite-1 zeolite membrane, is simple simultaneously, can be used for chip carriers, also can be used for tubular carriers and multi-channel carriers, is suitable to prepare the silicalite-1 zeolite membrane in a large scale, and is convenient for application and promotion. The method for preparing the high performance silicalite-1 zeolite membrane is beneficial to reduce preparation cost, and supplies necessary technical support to urge large-scale preparation of the high performance silicalite-1 zeolite membrane to achieve at an early date.

The invention discloses a preparation method of homocyclic peptide Cyclo-[(Asn)5-Cys]. The method includes: (1) connection of resin with protective group equipped asparagine to form asparagine connected resin; (2) sequential condensation of the asparagine connected to resin with the protective group equipped asparagine and cysteine to form linear peptide connected resin; (3) cutting of the linear peptide off the resin and end-to-end cyclization to obtain a cyclic peptide crude product; and (4) purification and preservation of cyclic peptide. The Cyclo-[(Asn)5-Cys] provided by the invention is a homocyclic peptide structure, has a similar structure to crown ether compounds, and compared with the heterocyclic peptide, has better regularity in terms of molecule structure, is easier for self-assembly into an ion channel or nanotube, thus serving as a drug carrier, membrane channel, molecular device and the like. The five amino functional groups contained on a side chain endow the product with certain water solubility and bioactivity, and a mercapto functional group contained on the side chain endows the product with better reaction activity, so that the product can be used as a precursor reagent for high efficient click reaction.

The invention relates to the technical field of medical apparatuses and instruments, in particular to a device for security detection of an epidural anesthesia puncture. The device comprises a valve body, a valve core and a testing membrane. The valve body is a four-way valve body. The testing membrane is arranged at a port of a testing membrane channel in a sealed mode. The valve core comprises a valve core body and a valve rod. The valve core body is tightly installed inside the valve core channel in a sleeved mode. The valve rod can rotate around the valve core channel. Three valve core through holes are formed in the valve core body. When the device is at the injection position or the closed position or the puncture position, the corresponding channel can be arranged at the required closed position by rotating the valve rod. The invention further discloses a method for the security detection of the epidural anesthesia puncture. According to the method and device for the security detection of the epidural anesthesia puncture, whether an anesthesia puncture needle punctures a ligamentum flavum can be visually and effectively detected and prompted, the anesthesia puncture needle reaches an epidural space, pain, caused by puncture failure, to a patient is avoided, operation and teaching by a clinician are also facilitated, and the visual and clear evidence is provided for medical confirmation of complications in anesthesia.

A protective device for a membrane of a sensor that detects a physical parameter acting upon the membrane includes a hollow main body that elongates in a direction along a longitudinal axis. The main body is open at one opposite end of the main body along the longitudinal axis, and at the end of the main body opposite the open end the protective device includes a bottom in which is defined a passage through which the medium is able reach the membrane when the protective device is attached to the sensor. The passage is defined in part by a wall that is configured so that the electromagnetic radiation propagating in the passage cannot reach the membrane without being reflected at least once on the wall.