47results about How to "Effective elution" patented technology

The invention relates to a cleaning method of a reverse osmosis membrane. By aiming at reverse osmosis membrane elements polluted by polyacrylamide, chemical agents and cleaning devices are used for realizing various cleaning modes of continuous cleaning and intermittent cleaning, positive cleaning and opposite cleaning, acidic cleaning agent and alkaline cleaning agent alternate using, and the pure water forward osmosis flushing. The cleaning method has the characteristics that the cleaning agent preparation is simple, the cleaning operation process is easy to control, the cleaning condition is mild, the reverse osmosis membrane element is not damaged, the performance of the reverse osmosis membrane is efficiently recovered, and the like.

The invention discloses an ultrafiltration membrane chemical cleaning method and aims at an ultrafiltration membrane that is used for processing purified PTA waste water. The invention uses an acid lotion prepared by citric acid and sodium bisulfite for dissolving and eluting inorganic pollutants that are formed by cobalt and manganese ion hydroxide, deposited and absorbed on the surface of the ultrafiltration membrane, and uses an alkaline oxidative lotion that contains sodium hypochlorite for dissolving and eluting organic colloid pollutants that are deposited and absorbed on the surface of the ultrafiltration membrane. The ultrafiltration membrane chemical cleaning method can effectively elute the pollutants, recover the running flux and running pressure of the ultrafiltration membrane, and ensure stable running of the purified PTA waste water in an ultrafiltration process.

The invention relates to a chemical cleaning method of a reverse osmosis membrane. In the method, aiming at a reverse osmosis membrane for processing PTA refining wastewater, inorganic pollutants which are formed by cobalt ions, manganese ions, hydroxide thereof and the like and are deposited and adsorbed on the surface of the membrane are dissolved and eluted by using acid rinse prepared by citric acid and sodium bisulfite; organic colloid pollutants deposited and adsorbed on the surface of the membrane are dissolved and eluted by using alkaline rinse containing sodium dodecyl benzene sulfonate and sodium hydroxide. The method can elute the pollutants on the surface effectively, restore operating flux, operating pressure and desalination rate of the reverse osmosis membrane, and ensure that the process of processing the reverse osmosis membrane for the PTA refining wastewater is stable in operation.

The present invention provides an eluent for repairing contaminated soil. The eluent comprises, by weight, 50-70 parts of a nonionic biosurfactant and 5-30 parts of an anionic surfactant. The compounding synergism of the nonionic biosurfactant and the anionic surfactant enhances the solubilizing effect of the eluent on organic pollutants in soil, especially polycyclic aromatic hydrocarbons, so theelution efficiency of the eluent is significantly improved; and the components of the elutent are biodegradable, so the eluent does not cause secondary damage to the environment in the use process, and is an efficient, clean and environmentally-friendly in situ eluent for repairing contaminated soil. The eluent provided by the invention is firstly foamed in the use process, so the spreading efficiency of an eluent solution in the soil medium is increased, the migration of the eluent in the horizontal direction is enhanced, and the uniformity and the repairing efficiency of the eluent in the soil are further improved.

The invention discloses a high performance liquid chromatographic analysis method for simultaneous determination of the content of 14 kinds of vitamins; octadecylsilane bonded silica gel is used as a filling agent, a 0.1 M sodium acetate solution-methanol is used as a mobile phase for gradient elution, gradient flow velocity of the mobile phase and a program change wavelength of an ultraviolet detector are combined, and intact separation and quantitative analysis of the 14 kinds of vitamins (including 4 kinds of fat soluble vitamins and 10 kinds of water soluble vitamins) can be achieved in 60 minutes. The method has the advantages of strong specificity, high precision, good stability, high accuracy, simple operation, and cheap and easily available reagents and consumable materials, is suitable for quality control of compound vitamin products in medicine and food industries, and can effectively save the cost and time of detection.

The invention discloses a method for extracting uranium by utilizing a photocatalysis technology under visible light. The method comprises the following steps: performing ultrasonic treatment on C3N4to make the C3N4 fully and uniformly dispersed, adding glucose and Cd(NO3)2.4H2O, performing magnetic stirring, adding L-cysteine, continuing stirring, moving the stirred material to a high-pressure reaction kettle, placing the obtained material in a drying oven, performing constant-temperature treatment, performing cooling, performing suction filtration, performing washing by using ultrapure water and absolute ethanol for a plurality of times, performing freeze drying, performing grinding, adding the ground material into a quartz tube, adding a UO2(NO3)2 solution and ultrapure water or seawater, introducing N2 for a period of time under the dark condition, placing the obtained material under natural light or a xenon lamp, performing irradiation for 10 min, filtering the suspension, adding1 mL of water into the obtained solid, placing the obtained material in air for 24 h, adding 1 mL of 0.1 mol/L Na2CO3 solution, performing desorption for 30 min, and performing filtration, wherein the extracted uranium is obtained in the supernatant. The method disclosed by the invention has the beneficial effects that 0.1 mmol/L uranium in 15 mL of seawater system can be completely extracted within 10 min under irradiation of the visible light.

The invention provides a preparation method of a Salvia miltiorrhiza soft capsule, comprising the following steps of: completely mixing Salvia miltiorrhiza extract with glycerine and polyethylene glycol 400 so that liposoluble constituents such as tanshinone II A are dissolved in a liquid substrate so as to achieve certain solubilization and improve the biological availability of liposoluble constituents of Salvia miltiorrhiza. In the preparation process of the Salvia miltiorrhiza soft capsule, a method for preparing Salvia miltiorrhiza water-soluble component extract is provided, a macroporous resin separation technology is adopted, and ethanol solution is concentrated, atomized and dried after elution; in the obtained extract, the total salvianolic acid compounds account for more than 60% of total solid, wherein the content of salvianolic acid compound danshinolic acid B is between 45% and 60%, and the content of danshensu is controlled between 10% and 20%. The content of danshensu is controlled while the yield of salvianolic acids is improved so that the proportion of the main active ingredient danshinolic acid B is obviously improved. The preparation method is safe and efficient, low-toxic and environmentally-friendly, can satisfy the requirement of industrial production, and has wide and practical application value.

The utility model discloses a novel mixed solid phase extraction column used for the preprocessing of pesticide residue; the column is characterized in that the column is filled with three fillers including octadecyl silane, aminopropyl and graphitized carbon; and the filling weight ratio of the octadecyl silane, the aminopropyl and the graphitized carbon is (120-300): (50-80): (4.5-7), preferably, is (150-250): 50: (4.5-7). The invention further discloses a preparation method and an application of the mixed solid phase extraction column. The leaching solvent used in the column is acetonitrile, which is the same as the extracting solvent generally used in pesticide residue detection, so that the purification step is simplified, the solvent exchanging step which is complex, and is possible to cause loss in the pesticide to be detected is eliminated, and the purpose of removing a plurality of interfere substances in only one column filtering process is fulfilled.

The invention relates to a functional pig dung modifier and a method for preparing a mushroom cultivation material by the utilization of modified pig dung. Raw materials of a modifier comprise, by weight, 60-80 parts of wormcast, 5-30 parts of calcium hydrate and 10-15 parts of potassium dihydrogen phosphate. The method comprises the following steps: adding the modifier into pig dung, adding a deodorant, uniformly stirring, carrying out a solid-liquid separation to prepare the modified pig dung, uniformly mixing the modified pig dung with cottonseed hull, soybean cake powder, lime powder, gypsum powder, light calcium carbonate, calcium superphosphate and urea, adding water and composting and fermenting so as to obtain the mushroom cultivation material. On the basis of maintaining acid-base balance of a system, the modifier, which is composed of wormcast, potassium dihydrogen phosphate and calcium hydrate, with the help of strong adsorption property of wormcast, effectively elutes viscous substances of pig dung and is beneficial to elution of substances such as antibiotics and heavy metals, etc. and is also conducive to desiccation and use of the raw materials. The mushroom cultivation material prepared by the above method has remarkable yield increase benefit and significant economic benefit.

The invention belongs to the protein chromatographic separation technology in the field of biochemical industry and discloses a mixed model chromatographic method for separating human serum albumin from yeast fermentation liquid. The method includes steps: 1) fermentation liquid pretreatment, to be more specific, removing yeast cells by centrifuging the yeast fermentation liquid which contains the human serum albumin, adding sodium caprylate, heating to remove impurity proteins and inactivating protease, performing centrifugal separation, and taking supernatant; 2) column chromatography, to be more specific, adopting a mixed mode medium with tryptophan as ligand, performing fixed bed chromatographic separation of the supernatant, and collecting elution peaks, wherein a sample loading pH value is 4.0-4.5, and an elution pH value is 7.0-9.0; 3) desalting and drying, desalting collected liquid, performing freeze drying to obtain the human serum albumin with purity larger than 95%. The mixed model chromatographic method is characterized in that by development of a novel separation process, the high-purity human serum albumin can be directly separated from the yeast fermentation liquid. By adoption of the mixed mode medium with tryptophan as the ligand, ion strength adjustment of the yeast fermentation liquid is avoided, adsorption under acid conditions and elution under neutral and weak alkali conditions are realized, and the method has advantages of mild elution conditions, simple process, high separation efficiency and high yield.

The invention belongs to the field of steroid hormone drug analysis methods, and particularly relates to an HPLC analysis method for estrone-related substances, wherein the main parameters comprise that a chromatographic column having an octyl silane bonded silica gel as a filler is selected, mobile phase gradient elution is performed, the mobile phase comprises water, methanol and acetonitrile, high performance liquid chromatography analysis is performed at an appropriate flow rate and an appropriate column temperature, and the chromatogram is recorded. According to the present invention, with the HPLC analysis method, the impurities in the estrone bulk drug can be subjected to effective elution, separation and quantitation, and can be completely separated from the main peak, and the analysis is rapid; and the method can be separately used for the detection of the related substance in estrone impurity A sample or estrone impurity D sample.

The invention discloses a detection method of a genotoxic impurity diisopropyl sulfate in a medicine. The detection method comprises dissolving a thymol drug raw material, carrying out extraction, carrying out centrifugal separation at a high speed to obtain a supernatant, and analyzing the supernatant through a triple tandem quadrupole high-performance liquid chromatography-mass spectrometer to detect the content of the genotoxic impurity diisopropyl sulfate in the thymol drug raw material. According to the detection method, a diisopropyl sulfate recovery rate is in a range of 90-110%, a standard deviation (RSD) is 2.33%, a detection limit is 1.74 micrograms/kilogram and a quantitation limit is 5.81 micrograms/kilogram. The method has a low detection limit, a high recovery rate, high sensitivity and good repeatability and can be used for detection of genotoxic impurity diisopropyl sulfate in pharmaceutical raw materials including drugs difficult to gasify.

The invention relates to a pretreatment method for measuring intracellular microcystin in natural water. The method comprises the steps of (1) carrying out microscopic examination before a water sample is filtered, and determining the quantity of needed water according to the turbid degree of the water sample and the detection effect of microcystis; (2) filtering the water sample, carrying out cell disruption after a filter membrane is cut into pieces, rotated in a vortex way and evenly mixed, and carrying out centrifugal separation to obtain supernate; (3) enriching the supernate containing microcystin in a solid-phase extraction column; and (4) eluting the microcystin enriched in the solid-phase extraction column, drying by blowing nitrogen, and making up to volume for testing. The method is simple, high in flexibility and high in operability, and is suitable for the pretreatment in the long-term measurement of intracellular microcystin in natural water at different seasons and in different areas.

The invention discloses a method for adsorbing and separating human serum albumin by expanded bed adsorption based on a mixed mode, which can directly separate and recombine human serum albumin from a yeast fermentation solution, and belongs to the protein separation technology in the biochemical field. The method comprises the following steps: (1) preprocessing a fermentation solution; obtaining the yeast fermentation solution containing the human serum albumin, adding sodium caprylate, and heating and deactivating protease; (2) performing expanded bed adsorption, directly separating the yeast fermentation solution by using an expanded bed filled with a mixed-mode adsorption agent, and collecting elution peaks; and (3) desalting and drying: desalting a collected solution, freeze drying to obtain the human serum albumin with the purity greater than 95 percent. The invention is characterized by developing a novel separation process, so that the high-purity recombined human serum albumin can be directly separated from the yeast fermentation solution. A critical point of the invention lies in adopting the expanded bed adsorption agent based on the mixed mode, cells do not need to be removed from the yeast fermentation solution, the ion strength does not need to be adjusted, the elution condition is moderate, and the method has the characteristics of simple process, high separation efficiency and high yield.

The invention belongs to the technical field of pesticide residue detection, and particularly relates to a detection method for determining various pesticide residues in non-fatty fruits and vegetables. The detection method for determining various pesticide residues in the non-fatty fruits and vegetables comprises the following steps: (1) extracting a sample; (2) purifying; (3) preparing a pesticide mixed standard; and (4) detecting. The detection method can effectively separate 53 pesticides in the non-fatty fruits and vegetables within 20 minutes, has a good correlation coefficient that R<2>is larger than 0.999 within a linear range, is simple to operate, quick to extract, high in accuracy and strong in repeatability, and is suitable for the confirmation analysis of the residual quantity of various pesticides in the non-fatty fruits and vegetables.

The invention discloses a method for purifying liraglutide, which comprises the following steps: dissolving and filtering crude peptide, then purifying with reversed-phase HPLC, in the purification process, washing with buffer salts A1 and B with low pH values as mobile phases, strictly controlling the temperature of a preparative column and the mobile phases at the moment, carrying out gradient elution for a certain time, and keeping the sample not eluted on the preparation column; then replacing a mobile phase with A2 and B with high pH values for washing, simultaneously changing the temperature and gradient of the preparative column, strictly controlling the temperature of the preparative column and the temperature of the mobile phase at the moment, carrying out gradient elution with B, and collecting qualified fractions; and desalting the collected qualified fractions, concentrating, and freeze-drying. According to the method, the maximum impurity removal effect can be achieved through one-step purification, qualified samples can be collected only through one-step purification, the quality and yield of products are improved, meanwhile, waste liquid can be reduced, and the method is environmentally friendly and beneficial to industrial production.

The invention discloses a method for extracting tripterine. According to the present invention, Fagopyrum plants are used as a raw material, liquid nitrogen grinding, enzymolysis treatment, ultrasonicextraction, extraction, silica gel column separation and recrystallization are sequentially performed, and the finally extracted tripterine has characteristics of high yield and high purity; and themethod of the present invention provides the new raw material and the extraction method for the tripterine extraction process, has the low raw material cost, and is suitable for industrial production.

The invention relates to a solid phase extract method of using the diamond alkyl ramification as elution reagent ingredient which contains link dextrin solid phase extract load body, belongs to the related separation enrichment chemistry and the solid phase extract processing of the chemical operation technology domain. The step of the invention method is as follow: (1) activating the solid phase extraction column which contains the link dextrin load body, flushing the extraction column by methyl alcohol or two distilled water; (2) taking the sample, joining the sample solution in the solid phase extraction column, and forcing the solution into the fixed phase, and keeping the sample analysis on the fixed phase; (3) drip washing, with two distilled water and so on drip washings, washing off the sample component which does not need or abbreviates this step; (4) eluting reagent which contains the diamond alkane ramification through the solid phase extraction column, and eluting the wait analysis from the link dextrin solid phase extract load body; (5) examination analysis, the eluent which collects from the above directly carries on chromatogram, electrophoresis and analyses, then computing and analyzing the obtained detection curve atlas, or the collection eluent uses in other research work.

Belonging to the technical field of water treatment, the invention in particular relates to a metal particle-bearing macromolecular curing agent. Metal particles are added in the process of pouring the macromolecular curing agent into a sheet, the metal particles can generate surface plasma wave after irradiation by a catalytic light source, and the metal particles include at least one or more ofcopper powder, cobalt powder and titanium dioxide, and have a particle size of 5nm-100microm. The invention also provides application of the metal particle-bearing macromolecular curing agent in watertreatment. The metal particle-bearing macromolecular curing agent provided by the invention has the advantages of simple structure, stable properties and long service life, and after water treatmenton the basis, micro-molecule cluster water with ultra-low viscosity and long-term storability can be prepared.