The invention belongs to the field of water bodypollution control, and provides a preparation method of a flavone sustained-release algal inhibition preparation. The flavone sustained-release algal inhibition preparation comprises 30% to 50% of 5,4'-dihydroxylflavone and 50% to 70% of embedding agent such as sodium alginate in percentage by weight, and has an entrapment rate of 50% to 70%. The preparation method of the flavone sustained-release algal inhibition preparation comprises the steps of: mixing a 5,4'-dihydroxylflavone dimethyl sulfoxide solution and a sodium alginate solution and mixing the obtained mixed solution and the mixed solution of chitosan and anhydrouscalciumchloride; and then after performing the reaction for a certain time, filtering to obtain a precipitated flavone sustained-release algal inhibition preparation. According to the preparation method, the used materials are high in ecological safety; the prepared flavone sustained-release algal inhibition preparation can obviously inhibit the growth activity of bloom algae, particularly inhibits the growth activity of microcystis aeruginosa, and can control or manage outbreak of algal bloom in lakes. Compared with the prior art, the flavone sustained-release algal inhibition preparation has durable action time, and is particularly suitable for preventing and managing the algal bloom which frequently breaks out.
The invention relates to a preparation method of an over-stable polyphase foam system, and especially relates to an environmentally friendly over-stable polyphase foam system and a preparation method thereof. The foam system contains a biotype surfactant alkylglucoside, a cationic gemini surfactant and silica nanoparticles, and the cationic gemini surfactant is C12-3-C12.2Br. The foam system has the advantages of good biocompatibility, good ecologic safety, great improvement of the foam stability, easy dispersion in the system by using hydrophilic nanometer, and easy preparation of foams. Only a very small amount of the cationic surfactant is needed by using the surfactant compounded system to foam, and the biotype surfactant alkylglycoside massively used in the system are cheap and easily available.
The invention discloses a method for preparing alga inhibitor from a natural plant barley, belonging to the field of water pollution control. The method comprises the following steps of: drying and crushing barley, extracting with ethanol containing water (or low-carbon alcohol containing water) to obtain barley extract liquid; distilling at reduced pressure to obtain an extract A; removing components which can be dissolved in petroleumether from the extract A; extracting with chloroform (or dichloromethane or diethyl ether) and ethyl acetate (or n-butyl alcohol or acetone) to respectively obtain organic phases B and C; and respectively distilling at reduced pressure to finally obtain alga inhibiting component extracts D and E. The method has the advantages that the method is simple and easy to operate, cost is low, material sources are wide and easy to obtain; moreover, two alga inhibitors with high purity can be obtained at the same time; therefore, the method can be widely used for controlling algal bloom of lakes and reservoirs.
The invention discloses an algae inhibiting method of donaxine plant in the pollution water control domain, which comprises the following steps: 1) extracting the algae inhibiting material in the donaxine plant by water and organic solvent; 2) loading the chopped bundle of donaxine plant or donaxine in the bag; placing the bag in the disposed water. The invention is fit for lake, reservoir, river, landscape aquifer, domestic fish pond, aquarium, miscellaneous natural aquifer and artificial aquifer, which avoids secondary pollution and aquifer landscape influenced by a great amount of the doted organics in the water.
The invention relates to a method and an application for extracting an alexandrium tamarense inhibitor from enteromorpha. The method comprises the following steps of: lixiviating per kilogram of the enteromorpha by using 2-5 liters of distilled water at room temperature for 12-72 hours, filtering to take filter liquor, and concentrating the obtained filter liquor into pastes by adopting a rotary evaporation method; dissolving the concentrated pastes by using a mixing solution of petroleumether and 90 percent of alcohol which are in a 1:1 ratio; sufficiently oscillating for 1-3 minutes, then demixing by standing for 10-120 minutes, and taking a lower-layer alcohol phase; decompressing, evaporating and concentrating the alcohol phase to obtain a paste-like substance at 40 DEG C; extractingand separating by using ethyl acetate and secondary distilled water which are in the 1:1 ratio, wherein a ethyl acetate phase is the alexandrium tamarense inhibitor; and determining the volume of thealexandrium tamarense inhibitor extracted by per kilogram of the enteromorpha to 50 milliliters. The invention is used for inhibiting the growth of alexandrium tamarense by adding 0.5-5 milliliters of the alexandrium tamarense inhibitor to per cubic metre of a water body, sufficiently utilizes the enteromorpha, has efficient economy, good ecological safety and easy operation and control, effectively inhibits the growth of the oceanic alexandrium tamarense and particularly inhibits the growth of gymnodinium breve.
The invention provides a base fluid for a biomassdrilling fluid and a preparation method thereof. The base fluid comprises, by weight, 60 to 90 parts of amino acidalcohol ester, 5 to 30 parts of polyetheramine alkylglucoside and 5 to 10 parts of methylsilicone oil. The above components are mixed so as to obtain the base fluid for the biomassdrilling fluid. According to the invention, amino acidalcohol ester has the advantage that the viscosity of amino acidalcohol ester is slightly influenced by temperature changes as the viscosity of ester substances, and has the characteristics of environment friendliness and biodegradability; through addition of polyetheramine alkylglucoside, the surface activity of the base fluid is substantially improved, so the base fluid has good ecological security and intermiscibility; and as methylsilicone oil is added, the base fluid has good lubricity, physiological inertness and chemical stability. Thus, the base fluid for the biomassdrilling fluid in the invention has the advantages of high environment friendliness, a wide viscosity range, a low freezing point, good lubricity, etc. and can meet performance demands for preparation of base oil for the drilling fluid. The invention also provides the biomass drilling fluid.
The present invention belongs to the field of water treating technology, and discloses the application of reed as algae inhibiting material. Reed as algae inhibiting material may be used through extracting algae inhibiting matter from reed with water or organic solvent and applying the extracted matter to the water to be treated; or setting bundled reed or reed in nylon bag into water to be treated. Utilizing reed as algae inhibiting material has the advantages of high algae inhibiting effect, low cost, easy control and high ecological safety. The present invention may be used in algae inhibition in natural or artificial water, such as lake, reservoir, river, park water, fish pond, etc.
The invention relates to an ecological safety type natural permeable reactive barrier medium and a preparation method thereof, and belongs to a reactive barrier medium and a preparation method thereof. The medium comprises the following raw materials in part by mass: 5 to 15 parts of cinder with the grain diameter of 0.1 to 5mm, 6 to 25 parts of turfy soil with the grain diameter of 0.1 to 2mm, and 5 to 30 parts of attapulgite with the grain diameter of 0.1 to 2mm, wherein both of the turfy soil and the attapulgite are subjected to heat treatment for modification, namely are heated for 1 to 3 hours at the temperature of 130+ / -2 DEG C. The novel compound permeable reactive barrier packing medium has the advantages of high efficiency, economy and high ecological safety, and can play an important role in the petroleum polluted groundwater control and repair process.
The invention relates to a method for preparing Enteromorpha prolifera extract for prohibiting red tide alga and the application thereof. And the preparing method: leaching Enteromorpha linza plants or their crushed matters with water, alcohol or acetone, aether, and chloroform or methanol, and concentrating the obtained extract by rotary evaporation, and throwing the concentrated extract into a to-be-processed water body to prohibit growth of red tide alga in the water body. And the extract of the Enteromorpha linza extract can be used to prohibit the red tide alga growing. Ordinarily, per kg Enteromorpha linza is leached with 30-50 L sterilized sea water for 1-3 days and the obtained Enteromorpha linza extract is applied to the to-be-processed water body, and 10-100 ml extract is thrown into per L water body and the prohibition rate is above 98%. And the invention can effectively prohibit harmful marine red tide alga.
The invention discloses a method for extracting an allelopathic subdivided component having an anti-algal activity from barley with petroleumether. The method comprises the steps of pre-treating barley straws and extracting for separation based on polarity, and separating to obtain a petroleumether phase having the anti-algal activity; drying the obtained extracting solution absolutely; then separating with a normal-phase silica column, wherein an eluent is composed by mixing petroleumether and ethyl acetate according to a volume ratio of 10:1; and thus the allelopathic subdivided component with relatively strong anti-algal activity is obtained. The method has the characteristics of simple and practicable operation, low cost and wide materials. With the method, the allelopathic subdivided component with relatively strong anti-algal activity can be obtained, and the allelopathic subdivided component can be widely used for controlling algal blooms in lakes and reservoirs.
The present invention discloses a method for extracting an allelopathy refinement component having algae inhibition activity from barley by using ethyl acetate. According to the method, barley straws are subjected to a pretreatment and extraction separation based on polarity to obtain an ethyl acetate phase having algae inhibition activity, and the obtained extraction solution is completely dried and separated by using a positive directionsilica gel column to obtain the refinement component having strong algae inhibition ability, wherein an eluant is prepared by mixing dichloromethane and methanol according to a volume ratio of 49:1. The method has characteristics of simpleness, easy performing, low cost, wide and easily-available materials and the like. With the method, the high purity barley allelopathy component having the algae inhibition activity can be obtained, and can be widely applicable for control of algae water blooms in lakes and reservoirs.
The invention relates to application of betel palm serving as an alga suppressing material. In the invention, a betel palm extract is put into a water body in which algae grow to realize suppression of algae. The betel palm has the advantages of quick response, good alga suppressing effect, easiness for operating, high ecological safety and the like, and can be applied to suppression of algae in natural water bodies or artificial water bodies such as lakes, reservoirs, rivers, garden landscape water bodies, household landscape water bodies and the like.
A method of inhibiting algae growth by using reed, belonging to water treating field, binding the reed plants or preparing reed powder, then loading to the nylon pocket, putting to the pretreated water body to inhibit the algae growth in water; after the algae growth is inhibited, getting the nylon basket out of water to prevent second pollution. The method is characterized by the good effect of algae inhibiting, low price of the material, ease to get, control, and ecological safety.
The invention discloses micromonospora haikouensis having algae dissolving capacity and an application of the micromonospora haikouensis to karenia mikimotoi, and belongs to the microbiology field fortreatment of harmful red tide. The algae dissolving fungi is named as Micromonospora haikouensis B2572 ( hereinafter referred to as Micromonospora haikouensis B2572 or bacterial strain B2572 ), separated from roots of sonneratia apetala in Maowei sea mangrovenature reserve in Qinzhou city of Guangxi province, and is preserved in China Center for Type Culture Collection at 22 May, 2019, with thepreservation No. of CCTCC NO:M 2019382. The new bacterial strain provided by the invention has good restraining and killing effect on Beibuwan bay sea area dominant algae namely the Karenia mikimotoi,under the condition of treatment for 24h, the algae dissolving rate can achieve 90% or above, and the micromonospora haikouensis has broad application prospects in harmful algal bloom events broken out in massive water bodies.
The invention relates to the anti-sticking agent technical field, and particularly relates to a metal surface treatment anti-sticking agent prepared from the following raw materials in parts by weight: 8-16 parts of hydroxyl silicone oil, 10-20 parts of polyaspartic acid, 5-10 parts of alkylglycoside, 8-18 parts of polytetrafluoroethylene, 5-10 parts of polyamideimide resin, and 10-20 parts of deionized water. The metal surface treatment anti-sticking agent is reasonable in composition, obvious in effect and stable in chemical properties, can effectively prevent adhesion between layers of a metal strip, and also has the function of corrosion resistance; the metal product surface quality is bright and clean; and the anti-sticking agent has good levelling property and thixotropy, meets processing at various temperatures, and can be used for a long time.
The invention discloses an emergency treatment method for quickly reducing water bloom hazards of a freshwater fishaquaculture pond. The method includes: adopting a physical method to drive most of cyanobacterial bloom in the freshwater fishaquaculture pond to a downwind position, performing purse seine to form a quasi-enclosure area; pouring biological flocculants to the quasi-enclosure area, completely pulling out cyanobacterial flocculation, then adding sustained-release microcystin degradation catalysts, and finally pouring a fish detoxifier with vitamin C serving as a main component into the aquaculture pond. By application of the method in emergency treatment of cyanobacterial bloom in the freshwater fish aquaculture pond, cyanobacterial biomass in aquaculture water can be remarkably reduced in extremely short time, microcystin in aquaculture water is effectively removed by catalytic degradation while immunity of cultured fishes is improved, and accordingly hazards caused by cyanobacterial bloom in the pond to the cultured fishes are reduced fundamentally. The emergency treatment method is efficient and high in ecological safety, and secondary injuries of the cultured fishes are avoided while hazards caused by cyanobacterial bloom in the pond to the cultured fishes are reduced sharply in short time. Therefore, the method is available for large-scale application in emergency treatment of cyanobacterial bloom in the freshwater fish aquaculture pond.
The invention discloses a method for promoting chlorella growth. The invention discloses application of pectinase and (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole in promoting algae growth. The pectinase is a common enzyme in the food field, and the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole is a substance capable of promoting pectinase activity, which can avoid influence on other organisms in environment. A using method has the characteristics that the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole is added to a water body when the chlorella grows to a certain stage, so that a promoting effect is taken when the chlorella just begins to grow to keep algae at a relatively high density and to effectively promote algae growth; the pectinase and the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole have the features of being safe, efficient, degradable and the like; and the pectinase and the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole can be extensively applied to a natural or landscape water body.
The invention discloses streptomyces thiogambosus, the preservation number of the streptomyces thiogambosus is GDMCC NO: 61327, and the streptomyces thiogambosus is classified and named as Streptomyces GXMD-hs-43. The strain is simple to screen and easy to culture, and the inventor also establishes a corresponding culture method. Researches show that the GXMD-hs-43 strain fermentation liquor has a very good prevention and treatment effect on citrus green mold, and the inhibition effect of the GXMD-hs-43 strain fermentation liquor on penicillium digitatum reaches up to 94.81% or above. Besides, a toxicology experiment of the GXMD-hs-43 strain shows that the GXMD-hs-43 strain is a non-toxic microorganism, so that the GXMD-hs-43 strain also has the characteristics of good ecological safety and the like, and secondary pollution cannot be caused in the use process. The discovery of the strain enriches the available microbial resources in China, and the strain has the advantages of stable antibacterial effect, high efficiency and environmental friendliness, and has good application prospects in prevention and treatment of citrus green mold.