Streptomyces thiogambosus and application thereof in prevention and treatment of citrus green mold

A technology for Streptomyces luteus luteus and green mold, applied in the field of microorganisms, can solve problems such as waste of social resources, material and financial resources, impact, and poor biological control

Active Publication Date: 2021-04-06
GUANGXI UNIV FOR NATITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on microbial control of diseases has achieved good results, but the commonly used antagonistic strains of diseases and insect pests are mainly broad-spectrum, and rarely involve in-depth research on a single disea...

Method used

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  • Streptomyces thiogambosus and application thereof in prevention and treatment of citrus green mold
  • Streptomyces thiogambosus and application thereof in prevention and treatment of citrus green mold
  • Streptomyces thiogambosus and application thereof in prevention and treatment of citrus green mold

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Experimental program
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Embodiment Construction

[0030] 1. Screening of strains

[0031] 1.1 Material preparation

[0032] Sample sampling: The soil sampling site is Guangxi Nonggang National Nature Reserve. The surface soil was removed, and soil samples at a depth of 15cm below the surface were collected, stored in a 4°C incubator, and brought back to the laboratory for further processing.

[0033] Separation and purification medium:

[0034] LB liquid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, distilled water 1000mL.

[0035] LB solid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, agar powder 15g, distilled water 1000mL.

[0036] PDA medium: 200.0g potato, 20.0g glucose, 20.0g agar, 1000mL distilled water.

[0037] 1.2 Isolation, purification and screening of strains

[0038] 1.2.1 Separation and purification

[0039] Add 2g of soil sample to 18ml of sterile water to prepare soil suspension, shake it on a shaker (180r / min) for 30min, and dilute it to 10% with sterile water. -1 ...

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Abstract

The invention discloses streptomyces thiogambosus, the preservation number of the streptomyces thiogambosus is GDMCC NO: 61327, and the streptomyces thiogambosus is classified and named as Streptomyces GXMD-hs-43. The strain is simple to screen and easy to culture, and the inventor also establishes a corresponding culture method. Researches show that the GXMD-hs-43 strain fermentation liquor has a very good prevention and treatment effect on citrus green mold, and the inhibition effect of the GXMD-hs-43 strain fermentation liquor on penicillium digitatum reaches up to 94.81% or above. Besides, a toxicology experiment of the GXMD-hs-43 strain shows that the GXMD-hs-43 strain is a non-toxic microorganism, so that the GXMD-hs-43 strain also has the characteristics of good ecological safety and the like, and secondary pollution cannot be caused in the use process. The discovery of the strain enriches the available microbial resources in China, and the strain has the advantages of stable antibacterial effect, high efficiency and environmental friendliness, and has good application prospects in prevention and treatment of citrus green mold.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to Streptomyces thioluteus and its application in preventing and treating citrus green mold. Background technique [0002] Citrus green mold is caused by Penicillium digitatum. Citrus green mold can infect citrus through various wounds on citrus. If tissue fluid seeps out, the infection rate of citrus green mold can be accelerated. Citrus green mold can survive on various organic substances. When a wound appears on the skin of a citrus, the conidia of the citrus green mold in the air will attach to the wound of the citrus and begin to infect the fruit. Citrus green mold is a common disease of citrus during storage, which often causes citrus fruit to rot and cause serious losses. [0003] At present, there are mainly three kinds of measures used to prevent and control diseases of citrus after picking: physical control, chemical control and biological control. [0004] ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23B7/155C12R1/465
CPCC12N1/20A23B7/155
Inventor 朱英芝姜明国王一兵黄文善卢杰刘艳黄群
Owner GUANGXI UNIV FOR NATITIES
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