54 results about "Penicillium digitatum" patented technology

The invention discloses a marine yeast for biologically preventing and treating postharvest diseases of fruits and vegetables, a method for preparing the same and application thereof. A marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is screened out from the seawater of the coastal waters of the East China Sea. The colony of the marine yeast is pink, has a smooth surface, and grows in a culture medium added with 1 to 2 percent NaCl. The use of a cell suspension of the yeast with certain concentration can effectively inhibit various postharvest main mycoses such as alternaria, botrytis cinerea, penicilllum expansum, penicillium digitatum, and pestalotia. The marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is used for biologically preventing and controlling the postharvest diseases of fruits and vegetables for the first time both at home and abroad. The strain has various functions of preventing decay, controlling oxidative stain, controlling softening flavour reversion and the like, and has the advantages of convenient use, no generation of antibiotic, no environment pollution, security, innocuity, and broad market prospect. At the same time, the strain is conducive to the export for earning foreign currency of China's high-quality fruit and vegetable products, and has significant economic and social benefits.

The invention discloses a strain of streptomyceslavendulae X33, and the preservation number is CCTCC No. M2013163. The bacterial strain and the fermentation liquid thereof can be used to prevent and treat vegetable penicillium digitatum, penicilliosis, sour rot disease, and canker disease and shoot blight of trees caused by botryosphaeriaspp. The streptomyceslavendulae X33 has the advantages of no toxic or side effect, simple preparation technology, low cost, and user-friendliness, is capable of preventing and treating various plant diseases at the same time, and has a prominent preventing and treating effect.

The invention discloses muscodor sp.W-S-41, the preservation number of which is CCTCC NO: M2016759. The muscodor sp.W-S-41 can inhibit plant pathogenic fungi comprising Botrytis cinerea ZAU10, Pestalotiopsis sp. ZAU20, Fusarium sp. ZAU 28, Penicillium digitatum KH08, Pythium ultimum ZAU22 and the like.

The invention discloses a Pichia manshurica for controlling postharvest diseases of oranges and grapes, and belongs to the field of biological control of postharvest diseases of fruits. A strain is identified as Pichia manshurica, and is named as Pichia manshurica Y4, and the preservation number of the Pichia manshurica Y4 is CGMCC 2.5415. When the Pichia manshurica is used, the Pichia manshurica is activated, cultured and centrifuged to obtain thalli, and the thalli are diluted with sterile water to prepare a thallus suspension with the concentration of 1.0*10<8>/mL. The suspension is sprinkled to the exocuticle of the fruits through using a watering pot, the fruits are air-dried, the dried fruits are put in a plastic basket, the plastic basket is sealed with a preservative film, and the sealed fruits are stored under room temperature conditions. The incidence of orange Penicillium digitatum, grape Aspergillus niger and gray molds is substantially reduced after treatment with the Pichia manshurica. The Pichia manshurica can substitute chemical bactericides to prevent and control postharvest diseases of oranges and grapes, avoids harms of the chemical bactericides to people, and has substantial economic and social benefits.

The invention belongs to the technical field of environment-friendly waste water treatment, and particularly relates to a microbial water treatment agent for heavy metal containing organic waste water. The microbial water treatment agent for the heavy metal containing organic waste water is prepared from dolomite powder, medical stone powder, anabaena azollae powder, water hyacinth powder, cladosporium sphaerospermum, Indian mustard powder, citrobacter amalonaticus, penicillium digitatum, hydroxyapatite powder, zeolite powder, citric acid, succinic acid, dicranopteris linearis powder, rhus lacquer powder, horse radish peroxidase, suillus bovines, laccaria amethystea, phanerochaete chrysosporium, bacillus subtilis, trichoderma koningii, trichoderma viride, bacillus mojavensis and klebsiella variicola. The microbial water treatment agent is high in treatment efficiency, low in running cost, wide in pH and temperature condition range, high in adsorbent reproducibility and free of secondary pollution.

The invention discloses a preparation method for an active mixed extract of tea tree oil meal as well as the extract and application of the extract. According to the method, water or alcohol is adopted as a solvent to extract waste tea tree oil meal residue to prepare the active mixed extract. The active mixed extract has an inhibiting effect on pathogenic fungi of microbe diseases after various fruits and vegetables are harvested, particularly has an obvious in vitro bacteriostatic effect on zymad like anthracnose of banana, Mango and papaya, as well as penicillium and penicillium digitatum of citrus after the fruits are harvested, can prevent and control diseases of banana, Mango and orange after harvesting in vivo, has an excellent effect in pollution-free preservation of fruits and vegetables after harvesting, and can be used as a plant antifungal agent or a fruit and vegetable preservative.

A traditional Chinese medicine composition preventing stalk penicillium digitatum and sclerotinia sclerotiorum comprises active component cortex fraxini and its extract and active component cortex dictamni and its extract, the mass percentage is respectively as follows: cortex fraxini and its extract 30 to 90% and cortex dictamni and its extract 10 to 70%. The preparation thereof is soaked in ethanol, extracted by ultrasonic and concentrated. The bacterial inhibition rate of the preparation can reach 90%. The preparation has exact effect, long effective term, non-toxic and non side effect andlow production cost, and overcomes the problems that the present chemical pesticide is easy to induce the drug resistance of pathogenic bacteria, the chemical agent residual exceeds the standard and does not meet the non-pollution requirement.

The invention discloses a bacteriostatic agent, comprising the following components: 10-18 parts of geraniol, 20-25 parts of eugenol, 7-10 parts of palmitic acid and 100-150 parts of water. The bacteriostatic agent disclosed by the invention can effectively kill penicillium digitatum on filter of an air conditioner, has low production cost, and is suitable for application.

The invention discloses bifidobacterium adolescentis capable of inhibiting filamentous fungi and an application of the bifidobacterium adolescentis, and belongs to the technical field of biology. Theinvention provides a bifidobacterium adolescentis CCFM1108 strain, and the strain can inhibit filamentous fungi. Inhibition of filamentous fungi is specifically reflected in that after the strain is co-cultured with penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores, the germination rate of the penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores can be remarkably inhibited within 7 days; and after the fermentation supernatant of the strain is separately co-culturedwith penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores, germination of the penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores can be remarkably inhibited, and the spore germination inhibition rates can reach 99.88+/-0.66%, 92.23+/-0.53%, 88.83+/-0.82% and 99.25+/-0.62% respectively.

The invention discloses a method for anticorrosion and preservation of postharvest citrus. The method comprises the following steps: selecting sunny days to harvest citrus fruits with uniform sizes and colors and without mechanical wound and flaws; inoculating mature common citrus postharvest disease strains (penicillium digitatum and sour rot strains) to skins of the citrus fruits subjected to disinfection treatment, and then soaking the fruits with practical cinnamyl aldehyde-fruit wax solution (containing 2.5 percent tween-80) for 15-30 s after leaving to stand for 4 h; naturally airing, packaging and storing the citrus at room temperature. The method adopts the technical scheme of firstly performing surface inoculating on the citrus and then soaking the citrus with cinnamyl aldehyde-fruit wax solution, is novel in thinking, and breaks through traditional thinking. Cinnamyl aldehyde adopted belongs to an edible biological bacteriostatic agent, can effectively reduce the disease percentage of green mold and sour rot of citrus fruits, does not affect the quality of the fruits, and has a good application prospect in the field of anticorrosion and preservation of citrus.

The invention discloses an antibacterial composition containing boscalid and prochloraz-manganese chloride complex and application of the composition. According to the antibacterial composition, boscalid and prochloraz-manganese chloride complex serve as main effective components, and the weight ratio of boscalid to prochloraz-manganese chloride complex is (1-70):(1-50). The antibacterial composition can be used for preventing cereal, fruit tree and vegetable diseases, has a high synergistic effect, overcomes and delays virus insecticide resistance, is high in sterilization speed and long in persistence, reduces application cost, and is better in prevention effect compared with single medicament application. The composition can be used for preventing fungal diseases on crops, can be particularly used for preventing powdery mildew, gray mold, rape sclerotinia sclerotiorum, the rotten disease, garlic leaf blight, tobacco brown spot, pepper gray mold, watermelon blight, the mushroom damp disease, grape anthracnose, orange penicilliosis, penicillium digitatum, mango anthracnose and other fungal diseases, and is better in effect compared with single medicament application.

The invention discloses streptomyces thiogambosus, the preservation number of the streptomyces thiogambosus is GDMCC NO: 61327, and the streptomyces thiogambosus is classified and named as Streptomyces GXMD-hs-43. The strain is simple to screen and easy to culture, and the inventor also establishes a corresponding culture method. Researches show that the GXMD-hs-43 strain fermentation liquor has a very good prevention and treatment effect on citrus green mold, and the inhibition effect of the GXMD-hs-43 strain fermentation liquor on penicillium digitatum reaches up to 94.81% or above. Besides, a toxicology experiment of the GXMD-hs-43 strain shows that the GXMD-hs-43 strain is a non-toxic microorganism, so that the GXMD-hs-43 strain also has the characteristics of good ecological safety and the like, and secondary pollution cannot be caused in the use process. The discovery of the strain enriches the available microbial resources in China, and the strain has the advantages of stable antibacterial effect, high efficiency and environmental friendliness, and has good application prospects in prevention and treatment of citrus green mold.

The invention discloses an application of plant polyphenols in preparing a pesticide for preventing Penicillium digitatum after harvesting of citrus and relates to an application of tannin in preparing a pesticide resisting Penicillium digitatum. The adopted tannin is natural polyphenol compounds, has a better inhibition function on spore germination and mycelial growth of Penicillium digitatum of the citrus, can remarkably reduce occurrence of Penicillium digitatum after harvesting of citrus fruits, effectively prolongs the storage period of the citrus fruits, does not affect the quality of the citrus fruits and has a good application prospect in the field of preservation of the citrus fruits.

The invention relates to a novel sterilizing composition containing silthiopham. The sterilizing composition comprises an active component A and an active component B, wherein the active component A is selected from silthiopham; the active component B is selected from one of thiophanate-methyl and carbendazim; and the weight ratio of the effective active component A to the effective active component B is 1:80-60:1. After an auxiliary agent is added to the effective active components, the novel sterilizing composition can be prepared into wettable powder, water dispersible granules, suspending agents, suspension emulsion, water emulsion, microemulsion and used for controlling take-all disease of wheat, downy mildew, epidemic diseases, late blight, damping-off, powdery mildew, gray mold, early blight, fusarium wilt, sclerotinia, anthracnose, ring rot, penicilliosis and penicillium digitatum, is resistant in rain wash and has obviously synergic effect.

The invention discloses a primer sequence which is used to detect the resistance frequency of Penicillium digitatum to imazalil, which comprises three groups of primer. A sense primer of the first group is equipped with a base sequence of SEQ ID No.1 in the sequence table, and an antisense primer of the first group is equipped with a base sequence of SEQ ID No.2 in the sequence table; a sense primer of the second group is equipped with a base sequence of SEQ ID No.3 in the sequence table, and an antisense primer of the second group is equipped with a base sequence of SEQ ID No.4 in the sequence table; a sense primer of the third group is equipped with a base sequence of SEQ ID No.5 in the sequence table, and an antisense primer of the third group is equipped with a base sequence of SEQ ID No.4 in the sequence table. The primer sequence of the invention has very high specificity and lays the foundation of the entire detection system.

The invention provides a plant disease combined fungicide. Especially the pesticide composition with prochloraz manganese salt and propineb as active ingredients, prochloraz manganese salt is prochloraz-manganese chloride, which is a broad-spectrum fungicide of imidazoles, which acts by inhibiting the biosynthesis of sterols. It has a good control effect on a variety of crop diseases caused by ascomycetes and semi-known fungi. Compared with prochloraz, the drug has high safety and is not easy to cause phytotoxicity. It is suitable for mushroom brown rot and brown spot. Mango anthracnose, citrus penicillium, green mold, anthracnose, pedicle rot, banana anthracnose, cucumber anthracnose, etc. Prosenb is a quick-acting, long-lasting, broad-spectrum protective fungicide, which is effective against It has a good protective effect against downy mildew of vegetables, tobacco, hops and other crops, as well as early and late blight of tomatoes and potatoes. It can control cabbage downy mildew, cucumber downy mildew, tomato morning and evening blight, mango anthracnose, and also has a certain inhibitory effect on powdery mildew, rust and diseases caused by Botrytis. It is safe for crops at the recommended dosage.

The invention discloses synthesis of oligopeptide and application of oligopeptide in a medicine for inhibiting citrus rot-causing bacteria penicillium digitatum. The oligopeptide is formed by linearlyconnecting five amino acids, is prepared by a solid-phase synthesis method, and is confirmed by high performance liquid chromatography and mass spectrometry detecting. Activity experiment results prove that the novel synthetic peptide has a good inhibition effect on Penicillium digitatum (Penicillium digitatum) which is a post-harvest rot-causing bacterium of citrus. Under the same concentrationeffect of 1 mg/ml, the preservative can be equivalent to commercially available preservatives; compared with oligopeptide with extremely small structural difference, the oligopeptide has better activity and no hemolysis phenomenon, which indicates that the substance has no obvious toxic or side effect on the human body, and indicates that the synthetic peptide Synpep prepared by the technology hasobvious advantages, is a potential citrus preservative, and can be used in industrial and agricultural production on a large scale.

The invention discloses a bactericidal composition and a pesticide and application thereof. The bactericidal composition consists of prochloraz and cuppric nonyl phenolsulfonate in the weight ratio of 1:30-30:1, preferably 1:15-15:1. The pesticide consisting of the composition which serves as an active ingredient and auxiliary ingredients which are acceptable in pesticides can be used for preventing and controlling anthracnose of cucumbers, blue mould and penicillium digitatum of oranges, rice false smut and rice blast. Test results prove that the composition and the active ingredients of the pesticide can achieve an obvious synergetic effect, the application amount of each ingredient of the pesticide can be reduced effectively, the pesticide resistance of plants can be delayed, the effective duration is prolonged, the prevention and control application range is extended, and the promotional value is high.

The invention discloses preparation and an application of a citrus natural bacteriostatic protein CsLTP1, specifically discloses a codon-optimized non-specific lipid transfer protein CsLTP1 gene in rutaceae plants, and is characterized in that the gene includes a sequence shown in SEQ ID NO:2. By optimization of a codon of the bacteriostatic protein CsLTP1, a prokaryotic expression vector is constructed, recombinant expression, purification and identification of rCsLTP1 are performed, and the obtained rCsLTP1 protein has good thermal stability and acid-base stability, and has obvious inhibitory effect on pathogenic bacteria penicillium digitatum of postharvest green mold of citrus under a living body condition. Compared with the prior art, the plant-derived bacteriostatic protein CsLTP1 isa natural bacteriostatic agent which can be produced on a large scale, can significantly reduce the occurrence of postharvest green mold of citrus fruits, reduces the harm caused by chemical bactericides and has good application prospects in the field of antisepsis and fresh keeping of the citrus fruits.

The invention discloses Lactobacillus sucicola JT03 with the preservation number being CGMCC No. 17538 and application of the Lactobacillus sucicola JT03 in the preparation of chemicals inhibiting Penicillium digitatum and especially chemicals for preventing citrus fruit postharvest green mold caused by the Penicillium digitatum. The Lactobacillus sucicola JT03 has the advantages that the Lactobacillus strain resource library is enriched, the application range of the lactobacillus in the fruit and vegetable fresh-keeping field is expanded, and a new solution is provided for the prevention andtreatment of citrus fruit postharvest green mold.

The invention discloses a microorganism composite microbial inoculum capable of restraining mycelial fungi and an application of the microorganism composite microbial inoculum, belongs to the technical fields of biology and fermentation, and provides the microorganism composite microbial inoculum which can effectively restrain mycelial fungi of penicillium expansum, aspergillus niger, penicilliumroqueforti, penicillium digitatum and the like. The microorganism composite microbial inoculum consists of bifidobacterium adolescentis of which the preservation No. is GDMCC No.60925 and lactobacillus plantarum of which the preservation No. is CGMCC No.5494. A fermented feed prepared from the microorganism composite microbial inoculum is rich in nutrients, fragrant in odour, not liable to corruptand high in safety, and specific reflection lies in that the content of mould in the fermented feed prepared from the microorganism composite microbial inoculum is 0CFU/g, and after the fermented feed is placed at 30 DEG C for 15d, the content of the mould in the fermented feed prepared from the microorganism composite microbial inoculum is 0CFU/g.