6 results about "Penicillium digitatum" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus velezensis A-39 and application of the bacillus velezensis A-39 in prevention and treatment of postharvest diseases of citrus, and the preservation number of the bacillus velezensis A-39 is CCTCC NO: M 20252917. The bacillus velezensis A-39 generates soluble antibacterial substances, does not generate volatile antibacterial substances, and has good prevention and treatment effects on citrus green mold, citrus anthracnose and citrus black rot. The result of the embodiment shows that the strain A-39, the bacterial liquid, the fermentation liquid and the metabolite of the strain A-39 can obviously inhibit the mycelial growth of penicillium digitatum; the control effect of the strain A-39 on the penicillium citrinum induced by penicillium digitatum is 100%; under natural conditions, the morbidity of citrus anthracnose treated by the strain A-39 is 0.33%, and the morbidity of citrus black rot is 0.33%; the control effect of the strain A-39 after fruit picking under the bacterial liquid concentration of OD600 = 0.3 is 68.84%.

The invention provides a PEG / CaCl2 mediated Penicillium citrinum genetic transformation method, and belongs to the technical field of microbial genetic transformation, and the method comprises the following steps: adding a target gene segment into Penicillium citrinum protoplast suspension, and carrying out an ice bath reaction; the target gene segment is obtained by taking pJET-hph-02944 plasmid as a template and carrying out PCR (Polymerase Chain Reaction) amplification by adopting a hygromycin B resistance gene primer pair with gene sequences as shown in SEQ ID No. 1 and SEQ ID No. 2; adding a PTC (Positive Temperature Coefficient) buffer solution into the mixed system, uniformly mixing and standing; continuously adding a TB3 liquid culture medium containing ampicillin, and carrying out recovery culture; uniformly mixing the resuscitated protoplast with a molten TB3 solid culture medium, pouring a plate, and carrying out dark culture for the first time; then covering a TB3 solid culture medium containing hygromycin B and ampicillin, carrying out secondary dark culture to obtain a transformant, and transferring the transformant to a PDA plate containing hygromycin B for screening. According to the method, CaCl2-PEG is adopted for mediated transformation of Penicillium citrinum, the transformation efficiency is higher, and the transformation speed is higher.

The application discloses a plant Lactiplantibacillus plantarum Yi-BC5 with a preservation number of GDMCC No: 67799, which shows strong inhibition on Pectobacterium carotovorum and Penicillium digitatum in vitro and in vivo, can significantly reduce the incidence and lesion diameter of pepper bacterial soft rot and citrus green mold; the Lactiplantibacillus plantarum Yi-BC5 can produce extracellular polysaccharide and other antibacterial metabolites, and the bacterial body and / or bacterial metabolite can be used for preparing products for inhibiting Pectobacterium carotovorum and / or Penicillium digitatum, and can be used for preparing products for preventing and treating bacterial diseases (including but not limited to pepper bacterial soft rot) and / or fungal diseases (including but not limited to citrus green mold) of fruits and vegetables. The Lactiplantibacillus plantarum Yi-BC5 can simultaneously control bacterial diseases and fungal diseases of fruits and vegetables after harvesting, has excellent disease control performance, provides an excellent antagonistic bacterial resource for postharvest preservation of fruits and vegetables, and lays a foundation for subsequent development of a new type of microbial bactericide.

The invention discloses streptomyces murinus FLWZ-0001 and application of the streptomyces murinus FLWZ-0001 in prevention and treatment of fungal and oomycete diseases of crops. The streptomyces murinus FLWZ-0001 is preserved in the China Center for Type Culture Collection on September 5, 2024, and the preservation number of the streptomyces murinus FLWZ-0001 is CCTCC (China Center for Type Culture Collection) NO: M20241923. The strain and the fermentation liquor thereof have a remarkable inhibition effect on various plant pathogenic bacteria, can efficiently inhibit fungal pathogenic bacteria such as sclerotinia sclerotiorum, botrytis cinerea and penicillium digitatum, and can also efficiently inhibit phytophthora capsici and phytophthora nicotianae belonging to oomycetes. Pot experiments show that fermentation liquor of the strain has excellent prevention and treatment effects on multiple crop diseases such as sclerotinia rot of colza, black shank of colza, phytophthora blight of pepper, gray mold and the like. The invention provides a brand new microbial resource for developing a broad-spectrum, efficient and environment-friendly multi-target biopesticide, and has a wide application prospect.

The invention provides a postharvest fruit preservative and fresh-keeping composite bacteriostatic agent and a preparation method and application thereof, and belongs to the technical field of postharvest fruit fresh-keeping, the composite bacteriostatic agent is composed of at least two of ethyl protocatechuate, sodium silicate, disodium ethylene diamine tetraacetate and tamarind fruit kernel extracting solution; the concentrations of ethyl protocatechuate, sodium silicate and disodium ethylene diamine tetraacetate in the composite bacteriostatic agent are not lower than the EC50 concentrations of the ethyl protocatechuate, sodium silicate and disodium ethylene diamine tetraacetate to pathogenic bacteria, and the adding volume of the tamarind kernel extracting solution in the composite bacteriostatic agent is the same as the total volume of ethyl protocatechuate, sodium silicate and disodium ethylene diamine tetraacetate; the pathogenic bacteria are at least one of citrus sour rot fungi, penicillium digitatum, citrus green mold and citrus colletotrichum gloeosporioides. The compound bacteriostatic agent disclosed by the invention can ensure food safety and avoid the problem of environmental pollution; the compound bacteriostatic agent has a remarkable and specific prevention and treatment effect on main postharvest citrus diseases caused by citrus sour rot fungi, penicillium digitatum, citrus green mold and citrus anthrax, and can be used for developing a novel postharvest citrus preservative.