18 results about "Penicillium digitatum" patented technology

A preparation method of plant compound essential oil comprises the following steps: preparing mixed powder from dried lemon slices, mint and litsea coreana, carrying out carbon dioxide supercritical extraction, and collecting an extract; the preparation method comprises the following steps: dropwise adding a carboxymethyl-beta-cyclodextrin solution into a mixture of eugenol and thymol, carrying out ultrasonic treatment to obtain an ultrasonic mixed solution, standing, centrifuging, collecting supernate, and drying to obtain a white powdery inclusion compound; and adding lecithin, Tween 80, disodium pyrophosphate and deionized water into the extract, heating and stirring, continuing to add the inclusion compound, homogenizing to obtain a nano-emulsion, adding gelatin and propylene glycol, heating and preserving heat, and cooling to obtain the plant composite essential oil. The compound essential oil disclosed by the invention has antibacterial and antioxidant effects, and has relatively strong antibacterial ability on botrytis cinerea and penicillium germs, and the DPPH free radical scavenging rate is greater than or equal to 90%. According to the invention, the active ingredients of the essential oil are wrapped by carboxymethyl-beta-cyclodextrin and synergistically embedded by lipidosome through a nano-embedding technology to realize slow release, so that the antioxidant and antibacterial properties are effectively prolonged.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus velezensis A-39 and application of the bacillus velezensis A-39 in prevention and treatment of postharvest diseases of citrus, and the preservation number of the bacillus velezensis A-39 is CCTCC NO: M 20252917. The bacillus velezensis A-39 generates soluble antibacterial substances, does not generate volatile antibacterial substances, and has good prevention and treatment effects on citrus green mold, citrus anthracnose and citrus black rot. The result of the embodiment shows that the strain A-39, the bacterial liquid, the fermentation liquid and the metabolite of the strain A-39 can obviously inhibit the mycelial growth of penicillium digitatum; the control effect of the strain A-39 on the penicillium citrinum induced by penicillium digitatum is 100%; under natural conditions, the morbidity of citrus anthracnose treated by the strain A-39 is 0.33%, and the morbidity of citrus black rot is 0.33%; the control effect of the strain A-39 after fruit picking under the bacterial liquid concentration of OD600 = 0.3 is 68.84%.

This invention belongs to the field of microbial technology, specifically relating to an Kodamaea strain HBF12 and its applications. The Kodamaea strain HBF12 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC M 20241462. Verification through examples shows that this strain has significant inhibitory effects on *Geotrichum citri-aurantii* and *Penicillium digitatum*, and exhibits good preventative effects against postharvest diseases of citrus, particularly sour rot and green mold. Strain HBF12 demonstrates good biocontrol potential, providing a new resource for the safe control of postharvest diseases of citrus.

The invention provides a PEG / CaCl2 mediated Penicillium citrinum genetic transformation method, and belongs to the technical field of microbial genetic transformation, and the method comprises the following steps: adding a target gene segment into Penicillium citrinum protoplast suspension, and carrying out an ice bath reaction; the target gene segment is obtained by taking pJET-hph-02944 plasmid as a template and carrying out PCR (Polymerase Chain Reaction) amplification by adopting a hygromycin B resistance gene primer pair with gene sequences as shown in SEQ ID No. 1 and SEQ ID No. 2; adding a PTC (Positive Temperature Coefficient) buffer solution into the mixed system, uniformly mixing and standing; continuously adding a TB3 liquid culture medium containing ampicillin, and carrying out recovery culture; uniformly mixing the resuscitated protoplast with a molten TB3 solid culture medium, pouring a plate, and carrying out dark culture for the first time; then covering a TB3 solid culture medium containing hygromycin B and ampicillin, carrying out secondary dark culture to obtain a transformant, and transferring the transformant to a PDA plate containing hygromycin B for screening. According to the method, CaCl2-PEG is adopted for mediated transformation of Penicillium citrinum, the transformation efficiency is higher, and the transformation speed is higher.

The application discloses a plant Lactiplantibacillus plantarum Yi-BC5 with a preservation number of GDMCC No: 67799, which shows strong inhibition on Pectobacterium carotovorum and Penicillium digitatum in vitro and in vivo, can significantly reduce the incidence and lesion diameter of pepper bacterial soft rot and citrus green mold; the Lactiplantibacillus plantarum Yi-BC5 can produce extracellular polysaccharide and other antibacterial metabolites, and the bacterial body and / or bacterial metabolite can be used for preparing products for inhibiting Pectobacterium carotovorum and / or Penicillium digitatum, and can be used for preparing products for preventing and treating bacterial diseases (including but not limited to pepper bacterial soft rot) and / or fungal diseases (including but not limited to citrus green mold) of fruits and vegetables. The Lactiplantibacillus plantarum Yi-BC5 can simultaneously control bacterial diseases and fungal diseases of fruits and vegetables after harvesting, has excellent disease control performance, provides an excellent antagonistic bacterial resource for postharvest preservation of fruits and vegetables, and lays a foundation for subsequent development of a new type of microbial bactericide.

The invention discloses a growth conditioner for planting tricholoma matsutake and a preparation method thereof, and belongs to the technical field of tricholoma matsutake planting.The growth conditioner for planting tricholoma matsutake is prepared from, by weight, 5-8 parts of penicillium citrinum metabolite, 2 parts of arbuscular mycorrhizal fungi with the spore content being 20-30 / g, 5 parts of bacillus subtilis 108-1010 CFU / g and 10-12 parts of matrix. The pH value of the saline-alkali soil can be reduced, meanwhile, the organic matter content of the soil is increased, and the method is suitable for planting tricholoma matsutake; the beta-glucosidase activity of the tricholoma matsutake is promoted, the biomass of cell walls of pine trees serving as host trees is easily utilized, biological energy is obtained, and the growth of the tricholoma matsutake is promoted.

The present invention discloses a bicycloalkane compound, a synthesis method and an application thereof. Using aryl dicyanoethylene and allyl carbonate as reaction raw materials, a bicycloalkane compound is obtained under the action of an organophosphorus catalyst. The advantages of the present invention include: high reaction efficiency and high yield; the organophosphorus catalyst is cheap, easily available, stable and has no pungent odor; no strong acid or strong base, as well as additional oxidizing or reducing agents need to be added during the reaction, and the conditions are relatively mild; no transition metal is used as a catalyst during the reaction, which is economical and environmentally friendly; the reaction substrates are easy to prepare. The compound represented by formula (III) of the present invention has good inhibitory activities against Penicillium digitaum and Rhizoctonia solani, and among them, the effect of compound 11 is the best, reaching 91% and 83% respectively.

The invention discloses golden yellow butterfly-shaped basidiospora zeng-LT01 and application thereof, and belongs to the technical field of microorganisms. The golden yellow butterfly-shaped basidiospora zeng-LT01 is separated from citrus fruits, is named as Papiliotrema aurea zeng-LT01, and is preserved in the China Center for Type Culture Collection on December 18, 2024, and the preservation number is CCTCC NO: M 20242841. The golden yellow butterfly-shaped basidiospora zeng-LT01 can be used for preparing the golden yellow butterfly-shaped basidiospora zeng-LT01. The golden yellow butterfly-shaped basidiospora zeng-LT01 has a remarkable antagonistic effect on penicillium digitatum and has a remarkable control effect on the postharvest penicilliosis of the citrus fruits, and a new solution is provided for preventing and treating the postharvest penicilliosis of the citrus fruits.

The invention discloses an antifungal hydrogel bead, which is prepared by the following steps: preparing a cyclodextrin metal organic framework (CD-MOF), packaging 2, 4-decadienal (DD) by using the CD-MOF to prepare DD / CD-MOF, and loading the DD / CD-MOF into a sodium alginate / egg white (SA / EW) hydrogel bead system to prepare the hydrogel bead DD / CD-MOF (at) AEW. Due to CD-MOF encapsulation, the stability of the DD is remarkably improved; due to the introduction of the DD, the stability of the CD-MOF in an SA / EW hydrogel system is also remarkably improved; the DD-loaded CD-MOF changes the crystallization behavior of SA / EW hydrogel beads and improves the crystallinity, so that the DD / CD-MOF coated AEW can better bear external force and dispersed stress and has better thermal stability and chemical stability, and the release of DD can be more stable and controllable. The hydrogel bead DD / CD-MOF (at) AEW disclosed by the invention has efficient antibacterial performance on penicillium digitatum, and the control effect on citrus fruit green mold and the fresh-keeping effect of citrus fruits after simulated transportation vibration are superior to those of hydrogel beads DD1 (at) AEW2: 1 reported in literatures.

The invention discloses streptomyces murinus FLWZ-0001 and application of the streptomyces murinus FLWZ-0001 in prevention and treatment of fungal and oomycete diseases of crops. The streptomyces murinus FLWZ-0001 is preserved in the China Center for Type Culture Collection on September 5, 2024, and the preservation number of the streptomyces murinus FLWZ-0001 is CCTCC (China Center for Type Culture Collection) NO: M20241923. The strain and the fermentation liquor thereof have a remarkable inhibition effect on various plant pathogenic bacteria, can efficiently inhibit fungal pathogenic bacteria such as sclerotinia sclerotiorum, botrytis cinerea and penicillium digitatum, and can also efficiently inhibit phytophthora capsici and phytophthora nicotianae belonging to oomycetes. Pot experiments show that fermentation liquor of the strain has excellent prevention and treatment effects on multiple crop diseases such as sclerotinia rot of colza, black shank of colza, phytophthora blight of pepper, gray mold and the like. The invention provides a brand new microbial resource for developing a broad-spectrum, efficient and environment-friendly multi-target biopesticide, and has a wide application prospect.

The present invention discloses a Bacillus amyloliquefaciens NTNK‑3 and its application. Bacillus amyloliquefaciens NTNK‑3 has been deposited in the Guangdong Provincial Microbial Culture Collection Center on July 6, 2022, and its deposit number is GDMCC No: 62606. The bacterium has the effect of inhibiting plant pathogenic fungi and weeds, and has an inhibitory effect on Colletotrichum gloeosporioides that causes anthracnose of pitaya and Penicillium digitatum that causes green mold disease of citrus; the germination inhibition rates of three weeds, barnyard grass, crabgrass and Amaranthus retroflexus, are 82.93%, 94.44% and 94.87% respectively, the root length inhibition rates of Setaria viridis and Amaranthus retroflexus are 96.2% and 91.84% respectively, and the stem length inhibition rates of Setaria viridis and Amaranthus retroflexus are 92.19% and 67.05% respectively.

The invention provides a postharvest fruit preservative and fresh-keeping composite bacteriostatic agent and a preparation method and application thereof, and belongs to the technical field of postharvest fruit fresh-keeping, the composite bacteriostatic agent is composed of at least two of ethyl protocatechuate, sodium silicate, disodium ethylene diamine tetraacetate and tamarind fruit kernel extracting solution; the concentrations of ethyl protocatechuate, sodium silicate and disodium ethylene diamine tetraacetate in the composite bacteriostatic agent are not lower than the EC50 concentrations of the ethyl protocatechuate, sodium silicate and disodium ethylene diamine tetraacetate to pathogenic bacteria, and the adding volume of the tamarind kernel extracting solution in the composite bacteriostatic agent is the same as the total volume of ethyl protocatechuate, sodium silicate and disodium ethylene diamine tetraacetate; the pathogenic bacteria are at least one of citrus sour rot fungi, penicillium digitatum, citrus green mold and citrus colletotrichum gloeosporioides. The compound bacteriostatic agent disclosed by the invention can ensure food safety and avoid the problem of environmental pollution; the compound bacteriostatic agent has a remarkable and specific prevention and treatment effect on main postharvest citrus diseases caused by citrus sour rot fungi, penicillium digitatum, citrus green mold and citrus anthrax, and can be used for developing a novel postharvest citrus preservative.

The invention belongs to the field of crop prevention and control technologies and pesticide residue treatment, and discloses a bacillus megaterium strain with multiple functions and application of the bacillus megaterium strain. The bacillus megaterium is bacillus megaterium ZIGUI6588, the preservation number is CCTCC (China Center For Type Culture Collection) NO: M20242075, and the bacillus megaterium can be used for preventing and controlling diseases such as citrus penicilliosis root-knot nematode and the like, has the function of degrading abamectin in fruits and soil, and has a wide application prospect in agricultural production.

The invention discloses application of a natural stilbene compound in preparation of a medicament for preventing and treating plant fungal diseases. A bacteriostatic activity research result shows that the natural stilbene compound provided by the invention specifically comprises pterostilbene, dihydroresveratrol, resveratrol trimethyl ether, polydatin and pine stilbene, and the polygonum multiflorum glycoside has bacteriostatic activity on four plant pathogenic fungi specifically comprising cacumen biotae, gleditsia sinensis wilt, walnut kernel mildew and rot bacteria and orange sour rot bacteria. Accurate toxicity determination results show that the toxicity of pterostilbene to the four pathogenic fungi is higher than that of commercially available medicaments such as prochloraz and chlorothalonil. Pharmacodynamic test results show that pterostilbene has good prevention and protection effects on citrus reiculata acid rot, and the prevention and treatment effects are significantly higher than those of prochloraz and chlorothalonil. Therefore, the natural stilbene compound provided by the invention is expected to be used for preparing a medicament for preventing and treating plant fungal diseases.

The invention discloses an application of a tamarind fruit core extract in fruit and vegetable preservation, the tamarind fruit core extract has an effect of inhibiting pathogenic bacteria causing fruit and vegetable spoilage, and experimental results show that the tamarind fruit core extract has a good inhibition effect on the pathogenic bacteria causing citrus spoilage, namely Penicillium digitatum. And the tamarind kernel extract is used for preserving the citrus, has no obvious influence on the quality of the citrus, is green, safe and reliable, is simple to prepare and low in cost, and is suitable for industrial production and market popularization and application.

The present invention discloses the application of rice leaf extract in inhibiting postharvest fungi of fruits and vegetables. The present invention provides the application of rice leaf extract in any one of the following: inhibiting postharvest fungi of fruits and vegetables; preparing a postharvest fungi inhibitor of fruits and vegetables; storing fruits and vegetables; preparing a product for storing fruits and vegetables. The rice leaf extract provided by the present invention can inhibit postharvest fungi of fruits and vegetables and has an obvious inhibitory effect on Alternaria alternata, Botrytis cinerea, Penicillium expansum and Penicillium digitatum. The present invention is of great significance for the postharvest storage of fruits and vegetables.

The invention relates to application of secondary metabolites of microorganisms, in particular to application of anhydride compounds in preparation of bactericide drugs. The molecular structure of the anhydride compound is a compound 1 (glaucic acid) and a compound 2 (glaucic acid) as shown in a formula I. Experiments show that the compound 1 and the compound 2 have remarkable inhibitory activity on penicillium digitatum causing citrus green mold and human pathogenic bacterium pseudomonas aeruginosa, and the anhydride compound is a bactericidal active compound with application prospects.