13 results about "Black rot" patented technology

The application discloses a marine source penicillium and a fermentation method and application thereof. The marine source penicillium is named Penicillium sp. DSF059, and the preservation number is CCTCC NO: M 20232310. Penicilliu The application optimizes the culture condition of the strain, selects a culture medium and culture condition with high yield of antibacterial active compounds to carry out fermentation, so that more target products can be obtained, the operation is simple, the cost is low, and meanwhile, the marine source penicillium Penicilliu Penicillium sp. DSF059 can provide an excellent strain for new drug development of resisting plant pathogenic fungi such as coffee leaf blight, coffee brown spot disease, grape gray mold disease, Chinese cinnamon brown spot disease, citrus black rot disease, pepper late blight, hickory leaf blight, coffee black spot disease and four pathogenic fungi separated from coffee leaf diseases and Staphylococcus aureus, and has a good application prospect for preventing and treating the plant pathogenic fungi and Staphylococcus aureus.

The application discloses application of BoDMR1 and BoDMR6 genes in cultivating disease-resistant broccoli varieties, an expression vector and a cultivating method, and relates to the technical field of plant molecular biology. A nucleotide sequence of a broccoli BoDMR1 gene is shown in SEQ ID No: 7, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 7; and a nucleotide sequence of a broccoli BoDMR6 gene is shown in SEQ ID No: 8, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 8. Through gene editing on endogenous BoDMR1 and / or BoDMR6 genes of plants, mutant plants showing resistance to diseases such as sclerotinia, black rot and alternaria blight can be obtained. Therefore, the application is beneficial to providing good resources for cultivating disease-resistant broccoli varieties.

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

The present application relates to a kind of parabacillus (Bacillus paralicheniformis) Bacillus paralicheniformis ) HMF14, the preservation number is CGMCC No.30480.The parabacillus HMF14 of the present application can resist salt and alkali, can simultaneously antagonize fusarium oxysporum, microdochium nivale var. avenae and xanthomonas campestris pv. tridii, also has toxic effect to pratylenchus penetrans and tetranychus cinnabarinus, can effectively prevent and treat wheat take-all disease and wheat black foot disease.

The invention provides application of mineral oil in improving the control effect of a copper preparation on broccoli black rot and relieving phytotoxicity, and belongs to the technical field of plant disease control. According to the invention, a 200-fold diluent of 99% mineral oil and a 200-800-fold diluent of 30% copper oxychloride suspending agent are compounded according to a ratio of 1: 1 to prepare a compound medicament, and the compound medicament can be applied to prevention and treatment of black rot of broccoli; the prevention and control method comprises the step of spraying the compound pesticide on leaf surfaces. The mineral oil and the copper oxychloride suspending agent are compounded to generate a synergistic effect, so that the prevention and treatment effect on the broccoli black rot is improved, the phytotoxicity caused by copper preparation stress is reduced, and the composition is resistant to rain wash, long in lasting period and suitable for efficient and green prevention and treatment of the broccoli black rot.

The invention belongs to the technical field of microorganisms, and particularly relates to pantoea agglomerans C-167 and application thereof in prevention and treatment of postharvest diseases of citrus. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 20252918 The pantoea agglomerans C-167 disclosed by the invention provides a novel high-quality strain resource for biological prevention and control of citrus green mold; meanwhile, a good prevention and treatment effect on citrus anthracnose and citrus black rot is also achieved. The strain C-167 can effectively reduce the use of chemical bactericides, reduce the risk of pesticide residues, and provide a new technical scheme for green prevention and control of postharvest diseases of citrus.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

The invention discloses a benzofuro [3, 2-d] pyrazolo [1, 5-a] pyrimidine compound as well as a synthesis method and application thereof, and relates to the technical field of organic synthesis and medicinal chemistry, the synthesis method of the compound comprises the following steps: in the presence of a catalyst and alkali, mixing a salicylaldehyde compound, a terminal alkyne compound and an alpha-aminoazole compound in a solvent for reaction, and separating to obtain the benzofuro [3, 2-d] pyrazolo [1, 5-a] pyrimidine compound. The benzofuro [3, 2-d] pyrazolo [1, 5-a] pyrimidine compound is obtained through the reaction. The furo [3, 2-d] pyrimidine compound is prepared by taking a salicylaldehyde compound, a terminal alkyne compound and an alpha-aminoazole compound as initial raw materials through a one-pot method, the reaction condition is mild, the raw materials are cheap and easy to obtain, the operation is simple and convenient, the production cost can be greatly reduced, and the production efficiency is improved; the furo [3, 2-d] pyrimidine compound has remarkable antifungal activity on plant pathogenic fungi, and can be applied to inhibition of fungi such as apple black beetle fungus and rhizoctonia solani.

The invention relates to the technical field of horticultural cultivation, in particular to a culture method for increasing the growth rate of crassulaceae ambrosia succulent plants. Comprising the following steps: selecting crassulaceae kalanchoe plants with developed root systems as stocks; after top cutting, naturally air-drying for 3-6 days at the temperature of 20-25 DEG C and the humidity of 70-80% to form a cut wooden bolt sealing layer; the method comprises the following steps: selecting a blister succulent plant with a hypocotyledonary axis as a scion; inserting the scion into a stock cut by adopting a cleft grafting method for fixing; and then placing in a short-day weak-light environment with the sunshine duration of 3-5 hours and the light intensity of 5000-7000lx for healing culture. According to the method, the technical bottleneck of extremely slow growth of blister succulent plants is thoroughly broken through through precise cooperation of strong nutrient pumping of the heterogeneity rootstocks, specific air-drying corrosion prevention and transpiration inhibition by weak light, black rot of grafted water is effectively avoided, the cultivation period is shortened to one year from several years, and the method has great large-scale production value.

The present application discloses a spizizenii bacillus and application thereof in pear tree disease prevention and treatment. The spizizenii bacillus strain of the present application exhibits significant antagonistic activity to escherichia amylor and apple black rot pericarp pear variety, and can be used as a core bacterial resource for biological prevention and treatment of pear tree diseases, thereby providing a new bacterial resource and technical path for constructing a green prevention and control technology system of pear tree diseases.

This invention relates to the field of biological pesticides, specifically disclosing a plant-derived fungicide combination for controlling black rot in broccoli. Its active ingredients are a compound of 0.2% benzoyl mycotoxin and 0.5% matrine. Under the technical solution of this invention, indoor fungicide toxicity tests show that both 0.2% benzoyl mycotoxin and 0.5% matrine have strong inhibitory effects on black rot in broccoli. When the mass ratio of 0.2% benzoyl mycotoxin to 0.5% matrine is 3:7 to 6:4, the co-toxicity coefficient against the broccoli black rot pathogen Xcc is greater than 120, exhibiting a synergistic effect. Indoor pot experiments show that when the mass ratio of 0.2% benzoyl mycotoxin to 0.5% matrine is 3:7 to 6:4, the control effect is significantly higher than that of 200 mg / L 15% agricultural streptomycin.

The application discloses a kind of rotten agent with disease prevention and control function and preparation method and application, belong to the cross field of agricultural microorganism technology and biological control.It is composed of compound bacteria powder and microcapsule fungicide by the rotten agent, the compound bacteria powder is made of bacillus subtilis ZKY01 bacteria powder and bacillus velezensis ZKY02 bacteria powder, and the microcapsule fungicide is bacillus siamensis ZKY03 bacteria powder embedded in microcapsule carrier;Wherein, the bacteria powder of three kinds of bacteria is the effective viable count not less than 1×10 11 CFU / g powder obtained by fermentation culture.The application can shorten the rotting period of agricultural organic waste such as straw, livestock and poultry manure to 12~15 days through the synergistic effect of three strains, and effectively prevent and control tobacco root black rot, black foot disease and other soil-borne diseases through the secretion of antibacterial substances, competition for nutrients and other mechanisms.

The application discloses a portable field tobacco black root rot pathogen rapid diagnosis and identification kit and belongs to the technical field of tobacco black root rot detection. The upstream primer, the downstream modified primer and the multi-site modified probe are disclosed, and the nucleotide sequences are respectively SEQ ID NO.1-SEQ ID NO.7; meanwhile, a simple kit for detecting tobacco black root rot is prepared by using the probe and the primer, and the problem that the detection of the tobacco black root rot pathogen is difficult to be carried out in the field without complex equipment is solved.