163 results about "Black rot" patented technology

The invention relates to a strain of lys-starch bacillus as well its application. The bacterium is separated from the soil on eggplant fields, anning district, Lanzhou, Gansu. The bacterium is identified to be Bacillus amyloliquefaciens. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus amyloliquefaciens I7 and preserving code of M206027. The broth of M206027 and M206027strain, non-cell culture fluid of M206027 strain's broth, and the monomer extracted from the M206027strain, M206027 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

The invention relates to bsacillus subtilis and microbial inoculum using the bsacillus subtilis as active ingredients, which are applied to the field of fruit freshness keeping. The bsacillus subtilis related by the invention is named as Bsacillus subtilis J18, which is preserved in China General Microbiological Culture Preservation and Management Center at present with the preservation number of CGMCC NO: 3665. Strains are prepared into fermentation agents after fermentation culture, and the fermentation agents are respectively mixed with calcium chloride and chitosan solution to be prepared into dry powder preparations and fruit glaze agents with obvious prevention and treatment effects on pear melasma after picking, black rot of pear, grape grey mould, watermelon wilt and the like. The bsacillus subtilis J18 belongs to a strain of antagonistic bacteria with wide-spectrum resistance on fruit and crop diseases.

The invention relates to a strain of Bacillus subtilis as well its application. The bacterium is separated from the soil on lily fields, fanjiaping, Lanzhou, Gansu. The bacterium is identified to be Bacillus-subtilis. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus-subtilis M22BH3 and preserving code of M206028. The broth of M22BH3 and M22BH3 strain, non-cell culture fluid of M22BH3 strain's broth, and the monomer extracted from the M22BH3 strain, M22BH3 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

Egg candling methods and apparatus are provided wherein black rot eggs can be identified and removed. A method of identifying live eggs includes illuminating eggs in a carrier with light from a light source; receiving light passing through each egg at a light detector; generating an output signal that corresponds to light received at a light detector for each respective egg; analyzing the output signal for each egg to determine opacity of each egg; removing eggs having an opacity less than a first opacity value; and removing eggs having an opacity greater than a second opacity value that is different from the first opacity value.

The invention discloses a special organic and inorganic compound pesticide fertilizer for tobacco. The special organic and inorganic compound pesticide fertilizer comprises the following components in parts by weight based on dry materials: 30-40 parts of organic active fertilizer, 12-20 parts of ammonium nitrate, 20-30 parts of calcium superphosphate, 16-32 parts of potassium nitrate, 5-10 parts of magnesium sulfate, 1-5 parts of zinc sulfate, 1-4 parts of sodium silicate, 0.1-1 part of borax, 0.02-0.1 part of ammonium molybdate, 0.5-1 part of insecticide, 0.5-1 part of bactericide and 0.5-1 part of coating agent. Compared with a conventional fertilizer, the special organic and inorganic compound pesticide fertilizer for the tobacco, produced according to the formula of the invention, has the advantages that 20-30 percent employees can be saved, the application of a pesticide is reduced, underground insects such as agrotis ypsilon and grubs, and root black rot can be controlled, a certain resistance is achieved for tobacco mosaic virus, the ratio of first-class tobacco is increased by 3.2-5.7 percent, and the output value is increased by 294.5-2512yuan / hm<2>.

The invention relates to an antagonistic bacterium for preventing the fungal diseases of broccoli as well as a preparation method and the application of a microbial preparation of the antagonistic bacterium, and belongs to the field of biological prevention and treatment of crop diseases. The invention provides a strain of brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622, a production method of a microbial preparation containing the brevibacillus brevis, and also the application of the brevibacillus brevis BBC-3 or the microbial preparation in preventing and treating the fungal diseases of the broccoli. The brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622 is obtained by virtue of preliminary screening and secondary screening, wherein a plate opposing test is performed for the preliminary screening and a liquid fermentation liquid is adopted for the secondary screening; the brevibacillus brevis BBC-3 is capable of effectively preventing and treating a plurality of fungal diseases of the broccoli, such as gray mold, downy mildew, stalk break, black rot and black spot, and has the prevention and treatment efficiency of above 70%; in addition, the brevibacillus brevis BBC-3 is good in environmental safety and has excellent development application prospect.

The invention discloses a bactericidal composition compounded by bromothalonil and methoxy acrylic acid esters, which is characterized in that: active components of the bactericidal composition are the bromothalonil (A) and one of strobilurin fungicides (B); the ratio of the weight percentage of the A to the B is 1:80-80:1; and a synergizing agent salicylhydroxamie acid (SHAM) is used in the composition. The active components account for 1 to 90 weight percent of the composition; and the synergizing agent salicylhydroxamie acid (SHAM) accounts for 1 to 50 weight percent of the composition. The composition has novel components, scientific compatibility and obvious synergism, solves the problem that the single bactericide has single action mode and high consumption and is easy to generate resistance, has the characteristics of high efficiency, low toxicity, environmental protection, and no risk of teratogenesis, carcinogenesis or mutagenesis, accords with the development direction of bactericides, can be widely applied to prevention and control of anthracnose, downy mildew and powdery mildew of vegetables and fruit trees, Mycosphaerella black rot, fusarium wilt, anthracnose, powdery mildew and downy mildew of melons, and plagues, and particularly has high effect on the anthracnose of various crops.

The invention discloses a pseudomonas nitroreducens and application thereof in degradation of DSF (diffusible signal factor) and / or DSF analogues. The pseudomonas nitroreducens disclosed by the invention is named as HS-18, and is preserved in China Center for Type Culture Collection on May 12, 2017 with a preservation number of CCTCC NO:M2017257. The strain has stable activity, can take DSF as thesole carbon source for growth, can completely degrade DSF within 24h, has a significant and rapid degradation effect on DSF, and has high application value in quorum quenching direction, black rot disease inhibition, and control of the harm of DSF mediation dependent pathogenic bacteria. The pseudomonas nitroreducens provided by the invention can reduce the pesticide abuse problem, and at the same time provides a new idea for biological control of the harm of phytopathogen.

The invention discloses Acinetobacter lactucae and an application thereof to degradation of a quorum-sensing signal molecule DSF (diffusible signal factor). The invention provides a bacterial strain of Acinetobacter lactucae QL-1, the bacterial strain is preserved in a China Center for Type Culture Collection on September 11th, 2017, and the preservation number is CCTCC NO: M2017487. The bacterialstrain can employ the quorum-sensing signal molecule DSF as a sole carbon source for growth, and 2mM of DSF signal molecules can be completely degraded in 15 hours with high degradation activity andstable degradation performance. The bacterial strain QL-1 has substantial biocontrol effects for black rot caused by Xanthomonas campestris XC1, and the bacterial strain also has characteristics of fast growth speed, simple cultivation method, good adaptation capability without easy variation, and great application potential for promotion of control of pathogens which are pathogenic depending on DSF; and at the same time, the product can reduce the problem of abuse of pesticides, and provides a new thinking for biological control of plant diseases.

The invention relates to a special medicated fertilizer for potatoes and a preparation method thereof. The medicated fertilizer is prepared by mixing organic fertilizer into a pesticide; the pesticide comprises the following components in parts by mass: 25-35 parts of 2% kasugamycin wettable powder agent, 25-35 parts of DT wettable powder agent, 15-25 parts of 50% phoxim emulsifying agent and 15-25 parts of chlorpyrifos emulsifying agent. As the fertilizer and the pesticide are organically combined together, the medicated fertilizer is more convenient to use. Due to the adoption of the special medicated fertilizer, underground pests such as potato black rot and cutworm and the like can be prevented obviously, and the rate of germination and commodity value of potatoes can be effectively improved, economic, social and ecological benefits are very obvious, and the application and popularization values are larger.

Disclosed is a continuous-cropping bacteria resistant agent. The continuous-cropping bacteria resistant agent is composed of bacillus licheniformis bacteria powder, streptomyces microflavus powder, pythium oligandrum yeasts, green wooden mold powder, edible fungi residues, compound sodium nitrophenolate and alpha-naphthalene acetic acid. By the arrangement, continuous-cropping diseases of crops can be alleviated, diseases of bacterial wilt, tobacco mosaic and the like caused by continuous cropping are highly prevented, various diseases of thielariopsis basicola, blight, root-knot nematode, damping off and the like can be prevented, and incidence rate and disease index of flue-cured tobacco black shank disease, the thielariopsis basicola and common mosaic are effectively reduced; by the aid of the continuous-cropping bacteria resistant agent, flue-cured tobacco can be promoted in early growth and quick tiller, growth period is brought forward, plant height and stem thickness of the flue-cured tobacco are increased, yield of flue-cured tobacco leaves is increased, and appearance quality of the tobacco leaves is improved.

The invention discloses a Pseudomonas aeruginosa strain capable of simultaneously controlling tobacco black shank and root black rot and having growth promoting effect and application thereof. The Pseudomonas aeruginosa is PA2101, Deposited at China Typical Culture Collection Center, Luojia Mountain, Wuchang, Wuhan, China, Deposited No.: CCTCC NO: M 2018546, Deposited on August 17, 2018. A biocontrol bacterium PA2101 capable of simultaneously controlling tobacco black shank and root black rot is firstly screened from tobacco field soil of Zhongzhuang Village, Fenchen Township, Xiangcheng County, Henan Province. The strain is identified as Pseudomonas aeruginosa, and has the function of promoting tobacco seedling growth. The experiment shows that the Pseudomonas aeruginosa has good inhibition effect on the pathogens of tobacco black shank and root black rot, and the control effect of the Pseudomonas aeruginosa on the tobacco black shank and root black rot can reach above 62%.

The invention provides applications of a gene relating to pathogenic mechanism of xanthomonas campestris pathovar campestris in prevention of crucifer black rot. The DNA sequence of a protein represented by sequence No.2 in a coding sequence table is the DNA sequence of the gene. More specifically, the DNA sequence of the gene is represented by sequence No.1. Gene XC2038 relating to pathogenic mechanism of xanthomonas campestris pathovar campestris is capable of providing drug targets for prevention of crucifer black rot.

The present invention discloses applications of a Xanthomonas pathopoiesis-associated gene hpaM in prevention and control of plant Xanthomonas diseases, wherein the number of the gene is XC_2847, the gene encodes a cell membrane protein, and the plant Xanthomonas diseases comprises one or a plurality of diseases selected from cruciferae black rot caused by cruciferae black rot bacteria, rice bacterial blight caused by rice bacterial blight bacterial, and rice bacterial leaf streak caused by rice bacterial leaf streak bacteria.

The invention provides a molecular detection primer for sweet potato black rot germs and an application of the molecular detection primer. The specific primer comprises an upstream primer DyB1F: 5'-TTCATCGTCAGGAATACCG-3' and a downstream primer DyB2R: 5'-GACCTTGGCTTTCTTGCTGTA-3';; and a specific amplification product with 509 bp of segment length can be amplified from pure DNA (deoxyribonucleic acid) of the sweet potato black rot germs, a tissue of a germ-carrying disease plant and soil by PCR (Polymerase Chain Reaction) amplification and agarose gel electrophoresis so as to detect the sweet potato black rot germs quickly. The specific molecular detection primer and the application thereof can be used for the quick, sensitive and specific detection of the sweet potato black rot germs in the germ-carrying soil, sick bodies, the disease plants and seedlings in the production practice, can also be used for the early diagnosis of field diseases and the monitoring and identification of the germs, and provides the control of the diseases caused by the sweet potato black rot germs with reliable technical and theoretical bases.

The invention relates to a preparation method of an insecticidal bacteriostatic agent, in particular to a household preparation and use method of a biological insecticidal bacteriostatic agent. The insecticidal bacteriostatic agent is prepared from, by weight, a component A and a component B. The component A is prepared from 25 parts of fresh citrus peel, 20 parts of fresh ginger, 10 parts of fresh pepper, 15 parts of fresh fig leaves and 30 parts of fresh mugwort. The component B is prepared from 85 parts of ethyl alcohol and 15 parts of ethyl acetate. The insecticidal bacteriostatic agent is easy to prepare, free of toxicity and high in practicality, the one-time killing rate of the insecticidal bacteriostatic agent for aphids, green leaf worms, red spiders, stem borers, scale insects, mites and leaf miners is more than 90%, and black rot, downy mildew, gray leaf spots, sooty mould, rotten diseases and powdery mildew can be effectively restrained.

The invention discloses microbial population sensing signal quenching bacteria and application thereof as biocontrol bacteria. Through study, the invention finds that Burkholderia cepacia has an obvious degradation effect on a population sensing signal molecule DSF; meanwhile, the Burkholderia cepacia F25 for efficiently degrading the DSF signal molecule is obtained by screening. The strain was preserved in the Guangdong Province Microbiological Culture Center on April 3, 2018, with a preservation number of GDMCC No:60346. The bacteria can quickly and efficiently degrade the DSF, and has population sensing and quenching functions and remarkable biocontrol effects on DSF pathogenic diseases such as black rot caused by xanthomonas campestris XC1. The microbial population sensing signal quenching bacteria disclosed by the invention can replace a chemical control method and eliminate potential safety hazards of antibiotic use, is environmentally-friendly and has important application valuefor preventing and treating plant diseases caused by pathogens relying on the DSF.

The invention provides a special preservative for Dongguan pear containing plant extracts, including vine tea extract, rosemary extract, honeysuckle extract, grape seed extract, chitosan, tea polyphenol, ε-polylysin Amino acid, starch, food-grade glycerin, water; the content of each component in each 1000ml preservative is: vine tea extract, 60-90mg, rosemary extract, 40-60mg, honeysuckle extract, 40-60mg, Grape seed extract, 20~40mg, chitosan, 3~5mg, tea polyphenols, 4~10mg, ε-polylysine, 3~5mg, starch, 10~100mg, food grade glycerin 1~2ml, water margin. The technical problem to be solved by the present invention is to provide a pollution-free preservative that reduces the water loss of Dongguan pears during refrigeration and normal temperature shelf life, reduces the incidence of black skin and rot, keeps the fruit surface clean and fresh, and has good fruit flavor.

The present invention relates to a method for making pulp with ginger seedlings and the pulp made by the method, which includes: putting ginger seedlings into water with black rot fungi and soft rot fungi, and rubbing the ginger seedlings with a twisting machine After twisting, wash the ginger seedlings with water; put them into a refiner for refining, add white rot fungi and brown rot fungi to the slurry, then use gravity separation to remove the sand in the slurry, squeeze dry, After drying and packaging into finished products, the whole process of the present invention does not produce any pollutants, does not require additional energy, and does not damage plant fibers. The produced pulp products do not contain any toxic chemicals and are completely green and environmentally friendly pulps. , has a great competitive advantage in the international market.

The invention discloses a breeding method of a cracking-resistant common head cabbage. The cracking-resistant common head cabbage bred by the method is bred by hybridization of a high cracking-resistant common head cytoplasm male sterile line of a female germplasm material and a cracking-resistant common head inbred line of a male germplasm material; and field disease resistance identification proves that: the racking-resistant common head cabbage variety has high virus disease resistance, black rot resistance and downy mildew resistance which are better than those of a check variety named Zhenqi. 100g (fresh weight) of the cracking-resistant common head cabbage contains the following components by weight: 1.2g of crude protein, 0.66g of crude fiber, 3.7g of soluble sugar, 28.7mg of Vc and 6.1g of dry matter. The cracking-resistant common head cabbage has obvious yield increasing superiority and stable yield which average 64303.5kg / hm and increases by 9.8% compared with the check variety Zhenqi.

The invention relates to trichoderma virens capable of preventing both tobacco black root rot and tobacco root rot and promoting tobacco growth, and belongs to the technical field of biological prevention and control. The trichoderma virens NKSF2019001F has been collected in China Center for Type Culture Collection, and the collection number of the trichoderma virens is CCTCC NO: M2019767; and theinvention further provides a screening method for the trichoderma virens and a biocontrol bacterium solution of the trichoderma virens. The strain can control both the tobacco black root rot and thetobacco root rot, and the control effect of the tobacco black root rot and the control effect of the tobacco root rot both reach 65% or more; the strain can effectively reduce the incidence rate of tobacco root and stem diseases, and is equivalent to existing common chemical pesticides in control effect; meanwhile, the strain further has the function of promoting the tobacco growth; and after being used, the trichoderma virens cannot generate resistance, cannot cause the problems of pesticide residues, environmental pollution and the like, is safe and environmental-friendly, and meets the requirement of ecological agriculture.

The invention discloses a compound microbial agent and application thereof in preventing and treating various plant diseases. The compound microbial agent has a remarkable inhibition effect on variousmain soil-borne diseases such as bacterial wilt, root rot, soft rot, black shank, gibberellic disease and root black rot caused by bacteria, fungi and oomycetes; meanwhile, the biomass of solanaceaecrop tomatoes, brassicaceous crop brassica campestris and cucurbitaceae crop cucumbers is remarkably improved, and a remarkable inhibiting effect is also achieved on tomato yellow leaf curl viruses which are plant virus diseases. The compound microbial agent effectively solves the problems of environmental pollution and food quality caused by the fact that the conventional microbial agent only aims at single pathogenic bacteria and chemical pesticide prevention and treatment, and has a good application prospect in green and environment-friendly and agricultural sustainable development.

Egg candling methods and apparatus are provided wherein black rot eggs can be identified and removed. A method of identifying live eggs includes illuminating eggs in a carrier with light from a light source; receiving light passing through each egg at a light detector; generating an output signal that corresponds to light received at a light detector for each respective egg; analyzing the output signal for each egg to determine opacity of each egg; removing eggs having an opacity less than a first opacity value; and removing eggs having an opacity greater than a second opacity value that is different from the first opacity value.

The invention discloses a pyraclostrobin-containing sterilization composition. The pyraclostrobin-containing sterilization composition comprises active ingredients including pyraclostrobin and iminoctadine tris(albesilate), the weight ratio of which is 1:10-8:1, preferably, 1:5-5:1. The composition can be prepared into emulsifiable solution, water dispersible granules, wettable powder, suspending agent, emulsion in water, microemulsion and microcapsule suspending agent allowable by agriculture. The pyraclostrobin-containing sterilization composition has a good control effect on main diseases such as sour rot, penicilliosis, penicillium digitatum, black rot, anthracnose, botrytis cinerea, grape gray mold and watermelon stem blight occurring in the storage period of citrus, and compared with the existing single preparation, the prevention effect is obviously improved, and the pyraclostrobin-containing sterilization composition has obvious synergism.

The invention relates to a paper pulp production technique which combines biolysis with machine work and has no used water discharging. It includes smashing the raw material, laying crack, biolysis, high-pressure pressing bursting, mediating, jordaning, pulp screening, and shoving. The used water can be used circularly to realize zero discharging. The paper pulp raw material includes straw, ditch reed, bamboo etc. The production technique has the advantages of no pollution, saving water, and low material consumption etc. And its cost is 40% of the chemical process. It is green high and new-technology industries with high economic benefit used to make food wrap paper, medicine wrap paper, and various papers.

The invention discloses a preparation method of triazolone. Triazolone is a triazole fungicide which is high in efficiency, low in toxicity, low in residue, long in lasting period and strong in internal absorption performance. Triazolone has very strong activity to some pathogenic bacteria in a living body, is very poor in in-vitro effect, has stronger activity to hyphae than the activity to spores, can be conducted in plant bodies after being absorbed by each part of a plant, has the effects of preventing, removing, treating and fumigating rust diseases and powdery mildew, and the like, and also has the effects on various crop diseases such as northern corn leaf spot, barley leaf blotch, wheat leaf blotch, pineapple black rot, maize head smut and the like.

The invention relates to a microbial three-in-one bactericide and aims at developing a novel green specific microbial pesticide for sterilization, growth promotion and disinsectization by carrying out refined fermentation on microbial bacteria preparation, plant source of sophora flavescens and tetramethyl pentane diacid growth regulating agent with three effects of sterilization, growth promotion and disinsectization to kill the mycoviruses of rice blast, black rot of cabbage, and the like. The microbial three-in-one bactericide comprises the following components in parts by weight: 60-90 parts of bacillus subtilis, 5-15 parts of photosynthetic bacteria, 5-15 parts of matrine, 0.3 part of tetramethyl pentane diacid, 0.05 part of organic silicon and 0.5 part of film forming agent. The preparation method comprises the following steps of: evenly mixing the materials according to a proportion, precipitating, filtering and packing into bags.

The invention belongs to the field of agricultural agents, and provides an agent composition capable of being used for preventing and curing black rot of tobacco roots and application thereof. The agent composition is prepared from an active ingredient A and an active ingredient B, wherein the active ingredient A is sodium selenite, and the active ingredient B is prochloraz or carbendazim; the weight ratio of the active ingredient A to the active ingredient B can be 1:(1-50). The agent composition has a very good effect of preventing and curing the black rot of the tobacco roots and can effectively retard the agent resistance and reduce the agent applied dosage.

The invention discloses a bird rape xanthomonas essential gene and coded protein and application thereof. The essential gene has one of the following nucleotide sequences: (1) a DNA (Deoxyribonucleic Acid) sequence of SEQ ID No.1 in a sequence table, and (2) polynucleotide for encoding the SEQ ID No.2 of an amino acid sequence in the sequence table. The protein coded by the essential gene has an amino acid sequence of SEQ ID No.2 in the sequence table. The essential gene or the coded gene thereof can be taken as a target for screening substance resisting bird rape xanthomonas. The essential gene and the coded protein thereof are essential for the growth of bird rape xanthomonas, so that the essential gene and the coded protein thereof can be taken as ideal and specific pesticide acting targets of bird rape xanthomonas. The crucifer black rot caused by bird rape xanthomonas is a severe hazard to the production of a large quantity of vegetables in the world, so that the research, the development and the application of the protein and the gene play a unique role in controlling the block rot.