25 results about "Black rot" patented technology

The invention discloses marine-derived penicillium as well as a fermentation method and application thereof. The marine source penicillium is named as Penicillium sp.DSF059, and the preservation number of the marine source penicillium is CCTCC (China Center For Type Culture Collection) NO: M 20232310. According to the invention, the culture conditions of the strain are optimized, the culture medium and culture conditions with high yield of antibacterial active compounds are selected for fermentation, more target products can be obtained, the operation is simple, the cost is low, and the method is suitable for industrial production. Meanwhile, the marine-derived penicillium sp. DSF059 can provide an excellent strain for the development of new drugs for resisting plant pathogens such as coffee leaf blight, coffee brown spot, grape gray mold, golden cypress brown spot, citrus black rot, phytophthora capsici, hickory leaf blight and coffee black spot as well as four pathogens separated from coffee leaf diseases and staphylococcus aureus; good application prospects are realized on prevention and treatment of phytopathogen and staphylococcus aureus.

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The application discloses a marine source penicillium and a fermentation method and application thereof. The marine source penicillium is named Penicillium sp. DSF059, and the preservation number is CCTCC NO: M 20232310. Penicilliu The application optimizes the culture condition of the strain, selects a culture medium and culture condition with high yield of antibacterial active compounds to carry out fermentation, so that more target products can be obtained, the operation is simple, the cost is low, and meanwhile, the marine source penicillium Penicilliu Penicillium sp. DSF059 can provide an excellent strain for new drug development of resisting plant pathogenic fungi such as coffee leaf blight, coffee brown spot disease, grape gray mold disease, Chinese cinnamon brown spot disease, citrus black rot disease, pepper late blight, hickory leaf blight, coffee black spot disease and four pathogenic fungi separated from coffee leaf diseases and Staphylococcus aureus, and has a good application prospect for preventing and treating the plant pathogenic fungi and Staphylococcus aureus.

The application discloses application of BoDMR1 and BoDMR6 genes in cultivating disease-resistant broccoli varieties, an expression vector and a cultivating method, and relates to the technical field of plant molecular biology. A nucleotide sequence of a broccoli BoDMR1 gene is shown in SEQ ID No: 7, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 7; and a nucleotide sequence of a broccoli BoDMR6 gene is shown in SEQ ID No: 8, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 8. Through gene editing on endogenous BoDMR1 and / or BoDMR6 genes of plants, mutant plants showing resistance to diseases such as sclerotinia, black rot and alternaria blight can be obtained. Therefore, the application is beneficial to providing good resources for cultivating disease-resistant broccoli varieties.

The invention discloses bacillus velezensis and application thereof in prevention and treatment of sweet potato black spot, and belongs to the field of microorganisms, 15 bacteria are successfully obtained through systematic separation of sweet potato rhizosphere soil and healthy plant tissues, screening is performed through a plate confrontation culture method, and the bacillus velezensis is obtained. The bacillus velezensis N-J-5 with a remarkable inhibiting effect on the sweet potato alternaria alternate is obtained. The antibacterial radius of the strain reaches 7.5 mm, and the antibacterial rate reaches up to 51.61%. And technologies such as morphological observation, physiological and biochemical characteristic determination, 16S rRNA gene sequence analysis and the like are comprehensively utilized to finally identify the bacillus velezensis as bacillus velezensis. The discovery of the strain provides a high-quality strain resource for research and development of a sweet potato black spot biological control preparation, and has important theoretical significance and practical value for promoting green and sustainable development of the sweet potato industry.

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

The present application relates to the technical field of pharmaceutical chemistry, and particularly relates to application of tryptophanone in prevention and treatment of plant bacteria and part of plant fungi. The present application discloses that natural product tryptophanone can effectively prevent and treat plant bacterial and part of plant fungal diseases. The application of tryptophanone as an agricultural bactericide in plant bacterial diseases has excellent inhibitory effect on rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwi canker, tobacco bacterial wilt, cucumber bacterial angular spot, potato bacterial wilt, soybean bacterial spot, tomato canker and the like. The tryptophanone also shows good inhibitory activity on plant pathogenic fungi such as grapevine Eutypa lata, tobacco alternaria alternata, eggplant verticillium wilt, pepper fusarium wilt, colletotrichum gloeosporioides, tea tree colletotrichum gloeosporioides and sorghum zylosporium.

The present application relates to a kind of parabacillus (Bacillus paralicheniformis) Bacillus paralicheniformis ) HMF14, the preservation number is CGMCC No.30480.The parabacillus HMF14 of the present application can resist salt and alkali, can simultaneously antagonize fusarium oxysporum, microdochium nivale var. avenae and xanthomonas campestris pv. tridii, also has toxic effect to pratylenchus penetrans and tetranychus cinnabarinus, can effectively prevent and treat wheat take-all disease and wheat black foot disease.

The invention provides application of mineral oil in improving the control effect of a copper preparation on broccoli black rot and relieving phytotoxicity, and belongs to the technical field of plant disease control. According to the invention, a 200-fold diluent of 99% mineral oil and a 200-800-fold diluent of 30% copper oxychloride suspending agent are compounded according to a ratio of 1: 1 to prepare a compound medicament, and the compound medicament can be applied to prevention and treatment of black rot of broccoli; the prevention and control method comprises the step of spraying the compound pesticide on leaf surfaces. The mineral oil and the copper oxychloride suspending agent are compounded to generate a synergistic effect, so that the prevention and treatment effect on the broccoli black rot is improved, the phytotoxicity caused by copper preparation stress is reduced, and the composition is resistant to rain wash, long in lasting period and suitable for efficient and green prevention and treatment of the broccoli black rot.

The invention relates to a biological seed coating agent and a preparation method thereof. The biological seed coating agent comprises a composite biological agent and a film-forming agent, the composite biological agent is a composite biological agent of bacillus polymyxa and bacillus megatherium. The film-forming agent comprises sodium alginate and polyvinyl alcohol; the mass ratio of sodium alginate to polyvinyl alcohol is (1-3): 2. The biological seed coating agent provided by the invention has an indoor and field control effect of more than 75% on radish black rot, has a remarkable promotion effect on growth of roots and stems of radish seedlings in an indoor test, and has a field test that compared with conventional control of farmers, the yield is increased by 1.6%, the commodity rate is increased by 3.3%, and the cost is saved and the efficiency is increased by 12%.

This invention discloses a comprehensive control method for black rot disease in cauliflower containing a biological agent, comprising the following steps: S1, transplanting treatment: 200-500 ml of the mixed biological agent is applied to the roots of transplanted cauliflower seedlings as a root drench; the mixed biological agent contains at least 1 × 10⁻⁶ viable bacteria. 8 S1. A bacterial suspension of *Penicillium restrictum* and *Paecilomyces lilacinus* at a CFU / mL ratio of 1-3:1; S2. Spraying treatment: After transplanting cauliflower seedlings, spray the plants with a compound microbial agent; the compound microbial agent contains a bacterial suspension of *Penicillium restrictum* and *Paecilomyces lilacinus* at a volume ratio of 4-6:1; the viable count of both *Penicillium restrictum* and *Paecilomyces lilacinus* bacterial suspensions is not less than 1×10⁻⁶. 8 CFU / mL. The method described in this invention can effectively reduce the incidence of black rot in cauliflower during the transplanting to survival period, survival to heading period, and heading to harvest period.

The invention belongs to the technical field of microorganisms, and particularly relates to pantoea agglomerans C-167 and application thereof in prevention and treatment of postharvest diseases of citrus. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 20252918 The pantoea agglomerans C-167 disclosed by the invention provides a novel high-quality strain resource for biological prevention and control of citrus green mold; meanwhile, a good prevention and treatment effect on citrus anthracnose and citrus black rot is also achieved. The strain C-167 can effectively reduce the use of chemical bactericides, reduce the risk of pesticide residues, and provide a new technical scheme for green prevention and control of postharvest diseases of citrus.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

The invention discloses a benzofuro [3, 2-d] pyrazolo [1, 5-a] pyrimidine compound as well as a synthesis method and application thereof, and relates to the technical field of organic synthesis and medicinal chemistry, the synthesis method of the compound comprises the following steps: in the presence of a catalyst and alkali, mixing a salicylaldehyde compound, a terminal alkyne compound and an alpha-aminoazole compound in a solvent for reaction, and separating to obtain the benzofuro [3, 2-d] pyrazolo [1, 5-a] pyrimidine compound. The benzofuro [3, 2-d] pyrazolo [1, 5-a] pyrimidine compound is obtained through the reaction. The furo [3, 2-d] pyrimidine compound is prepared by taking a salicylaldehyde compound, a terminal alkyne compound and an alpha-aminoazole compound as initial raw materials through a one-pot method, the reaction condition is mild, the raw materials are cheap and easy to obtain, the operation is simple and convenient, the production cost can be greatly reduced, and the production efficiency is improved; the furo [3, 2-d] pyrimidine compound has remarkable antifungal activity on plant pathogenic fungi, and can be applied to inhibition of fungi such as apple black beetle fungus and rhizoctonia solani.

The invention relates to the technical field of horticultural cultivation, in particular to a culture method for increasing the growth rate of crassulaceae ambrosia succulent plants. Comprising the following steps: selecting crassulaceae kalanchoe plants with developed root systems as stocks; after top cutting, naturally air-drying for 3-6 days at the temperature of 20-25 DEG C and the humidity of 70-80% to form a cut wooden bolt sealing layer; the method comprises the following steps: selecting a blister succulent plant with a hypocotyledonary axis as a scion; inserting the scion into a stock cut by adopting a cleft grafting method for fixing; and then placing in a short-day weak-light environment with the sunshine duration of 3-5 hours and the light intensity of 5000-7000lx for healing culture. According to the method, the technical bottleneck of extremely slow growth of blister succulent plants is thoroughly broken through through precise cooperation of strong nutrient pumping of the heterogeneity rootstocks, specific air-drying corrosion prevention and transpiration inhibition by weak light, black rot of grafted water is effectively avoided, the cultivation period is shortened to one year from several years, and the method has great large-scale production value.

This invention discloses a phenylaminothiazole derivative as shown in Formula I and its application in the control of plant diseases. The phenylaminothiazole derivative uses readily available raw materials, has a simple preparation method, convenient post-processing, and a high product yield. Furthermore, the compound shown in Formula I exhibits broad-spectrum and highly effective antifungal activity against plant fungi, particularly showing excellent bactericidal effects against rice sheath blight, rapeseed sclerotinia sclerotinia, tomato early blight, grape bud blight, apple black rot, wheat sheath blight, wheat scab, and colloidal anthracnose.

The present application discloses a spizizenii bacillus and application thereof in pear tree disease prevention and treatment. The spizizenii bacillus strain of the present application exhibits significant antagonistic activity to escherichia amylor and apple black rot pericarp pear variety, and can be used as a core bacterial resource for biological prevention and treatment of pear tree diseases, thereby providing a new bacterial resource and technical path for constructing a green prevention and control technology system of pear tree diseases.

The invention discloses application of a polyamine compound as an agricultural bactericide in plant bacterial diseases, plant fungal diseases and plant viral diseases. The composition is used for treating rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial leaf spot, kiwi fruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, potato bacterial wilt, soybean bacterial leaf spot, tomato canker, cucumber gray mold, pepper fusarium wilt, sclerotinia rot of colza, wheat scab and potato late blight. The compound provided by the invention has good inhibition effects on root rot of blueberries, botryosphaeria dothidea, pitaya anthracnose, rice sheath blight disease, fusarium SP or colletotrichum sorghum and tobacco mosaic virus. The polyamine compounds mainly comprise spermine and spermidine, are extracts of natural plants, and are environmentally friendly. The invention provides new application of the polyamine compound, the polyamine compound can be used for preventing and treating plant bacterial diseases, plant fungal diseases and plant viral diseases in the agricultural field, and a new way is provided for research, development and creation of new pesticides.

This invention relates to the field of biological pesticides, specifically disclosing a plant-derived fungicide combination for controlling black rot in broccoli. Its active ingredients are a compound of 0.2% benzoyl mycotoxin and 0.5% matrine. Under the technical solution of this invention, indoor fungicide toxicity tests show that both 0.2% benzoyl mycotoxin and 0.5% matrine have strong inhibitory effects on black rot in broccoli. When the mass ratio of 0.2% benzoyl mycotoxin to 0.5% matrine is 3:7 to 6:4, the co-toxicity coefficient against the broccoli black rot pathogen Xcc is greater than 120, exhibiting a synergistic effect. Indoor pot experiments show that when the mass ratio of 0.2% benzoyl mycotoxin to 0.5% matrine is 3:7 to 6:4, the control effect is significantly higher than that of 200 mg / L 15% agricultural streptomycin.

The application discloses a kind of rotten agent with disease prevention and control function and preparation method and application, belong to the cross field of agricultural microorganism technology and biological control.It is composed of compound bacteria powder and microcapsule fungicide by the rotten agent, the compound bacteria powder is made of bacillus subtilis ZKY01 bacteria powder and bacillus velezensis ZKY02 bacteria powder, and the microcapsule fungicide is bacillus siamensis ZKY03 bacteria powder embedded in microcapsule carrier;Wherein, the bacteria powder of three kinds of bacteria is the effective viable count not less than 1×10 11 CFU / g powder obtained by fermentation culture.The application can shorten the rotting period of agricultural organic waste such as straw, livestock and poultry manure to 12~15 days through the synergistic effect of three strains, and effectively prevent and control tobacco root black rot, black foot disease and other soil-borne diseases through the secretion of antibacterial substances, competition for nutrients and other mechanisms.

This invention relates to the field of microbial technology, specifically disclosing a highly effective Bacillus vallismortis strain for controlling root rot, a bacterial-drug compound, and its application. The Bacillus vallismortis strain is Bacillus vallismortis 81-8, deposited on April 1, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, with accession number CGMCC No. 34056, and classified as Bacillus vallismortis. The strain 81-8 of this invention exhibits in-plate inhibition rates of 69.39% and 48.53% against pepper blight and tobacco root rot, respectively. It can still grow on a medium containing 12% NaCl and has a significant growth-promoting effect on peppers. When combined with carbendazim, its control efficacy against tobacco root black rot reaches over 99%.

The invention discloses burkholderia sp. And application thereof, relates to the technical field of microorganisms and application thereof, and in particular relates to burkholderia sp., the strain number is A41, the burkholderia sp. Is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20232062. According to the method, burkholderia substances and fermentation products thereof are applied to sweet potato blocks, so that the disease development of the sweet potatoes infected by the alternaria brassicae can be effectively inhibited, and a relatively ideal prevention and treatment effect can be achieved. The burkholderia sp. Shows strong inhibitory activity on various important plant pathogenic fungi, especially has a hypha treatment inhibition rate of sweet potato black spot of a convolvulaceae plant sweet potato of 90% or more, also has significant prevention and treatment potential on gray mold and the like, has broad-spectrum biocontrol property, can save a large amount of cost, and has wide application prospects. The method has important application value in cultivation or postharvest preservation of economic plants.

The invention relates to bacillus paralicheniformis HMF14, and the preservation number of the bacillus paralicheniformis HMF14 is CGMCC (China General Microbiological Culture Collection Center) No.30480. The bacillus paralicheniformis HMF14 has the advantages that the preservation number is CGMCC No.30480; the bacillus paralicheniformis HMF14 disclosed by the invention can resist saline and alkaline, can antagonize fusarium oxysporum, hematoptera graminearum var. Graminearum and xanthomonas brassicae specialized wheat at the same time, also has a poisoning effect on pratylenchus agilis and paenchus calcarinus, and can be used for effectively preventing and treating wheat take-all and wheat black glume disease.

The application discloses a portable field tobacco black root rot pathogen rapid diagnosis and identification kit and belongs to the technical field of tobacco black root rot detection. The upstream primer, the downstream modified primer and the multi-site modified probe are disclosed, and the nucleotide sequences are respectively SEQ ID NO.1-SEQ ID NO.7; meanwhile, a simple kit for detecting tobacco black root rot is prepared by using the probe and the primer, and the problem that the detection of the tobacco black root rot pathogen is difficult to be carried out in the field without complex equipment is solved.