The invention relates to a strain of Bacillus subtilis as well its application. The bacterium is separated from the soil on lily fields, fanjiaping, Lanzhou, Gansu. The bacterium is identified to be Bacillus-subtilis. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus-subtilis M22BH3 and preserving code of M206028. The broth of M22BH3 and M22BH3 strain, non-cell culture fluid of M22BH3 strain's broth, and the monomer extracted from the M22BH3 strain, M22BH3 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.