This invention discloses a Is PETase mutants and their application in the degradation of PET plastics. This invention addresses existing... Is The PETase
mutant cannot tolerate high substrate (PET plastic) concentrations, thus providing a method to improve the degradation activity of PET plastic. Is PETase
mutant. For the aforementioned Is The present invention also optimizes the
reaction system of the PETase
mutant, thereby providing a method for depolymerizing PET plastics. Using the mutants described in this invention, such as M16-G, the degradation of high-concentration (20%) PET plastics can be completed at a Tg temperature, and it can be used for the degradation and recycling of PET plastics under industrial conditions. Furthermore, the present invention... Is PETase mutants, such as M16-G, can tolerate
seawater.
Seawater can be used to prepare the reaction buffer required for its degradation of PET plastics, which helps to save
freshwater resources and protect the environment.