15results about How to "High degradation activity" patented technology

The invention relates to the field of enzyme preparations, and discloses an enzyme preparation for catalytic degradation of bioplastics and a preparation method thereof, the enzyme preparation for catalytic degradation of bioplastics comprises the following components by weight: 25-50 parts of an enzyme, 1-8 parts of a catalytic additive, 2-10 parts of a stable dispersant, and 30-50 parts of a buffer solution; the catalytic additive is prepared by using calcium bentonite as a carrier to load nano titanium dioxide; the stabilizing dispersant is prepared by introducing polyethylene glycol monoallyl ether into a sodium lignin sulfonate structure by utilizing an initiator through an aqueous solution polymerization method, and by adding the catalytic additive and the stabilizing dispersant, the enzyme preparation is endowed with good degradation efficiency on bio-based plastics and the stability of an enzyme preparation system is improved.

The application belongs to the technical field of enzyme engineering, and discloses a BhrPETase mutant and application thereof. The application provides a BhrPETase mutant, wherein the amino acid sequence is that threonine at the 160th position in the amino acid sequence shown in SEQ ID No. 2 is mutated to tyrosine, and the amino acid residues at other positions are unchanged. The BhrPETase mutant provided by the application has a PET degradation activity 2.32 times that of wild-type BhrPETase and 39.4% higher than that of mutant F208I under the condition that the thermal stability of the BhrPETase mutant is basically equivalent to that of wild-type BhrPETase. The BhrPETase mutant provided by the application can be applied to the fields of degrading PET, recycling PET degradation products or preparing a PET degradation agent.

This invention relates to the field of wastewater treatment technology, specifically to a microbial preparation, its preparation method, and its application, comprising: a composite microbial agent; and a calcium alginate / polyvinyl alcohol interpenetrating network aerogel carrier loaded with the composite microbial agent; the aerogel carrier contains uniformly dispersed modified nano-attapulgite clay. The microbial preparation of this invention exhibits good wastewater treatment performance and long-lasting effect.

The invention relates to an azo dye degrading bacterium SHY-2 and application thereof, the strain can efficiently degrade azo dye acid red 73 and reactive black 5, in an inorganic salt culture medium only added with glucose, cane sugar, starch or glycerol as a carbon source, the highest degradation rates of 100 mg / L acid red 73 and reactive black 5 within 5 days can reach 98.7% and 95.3% respectively, an organic nitrogen source is not needed, and the treatment cost is low. A mixed flora SH formed by the strain and a serratia bacterium also has a remarkable synergistic degradation effect on two dyes, and complete decolorization can be realized within 48-60 hours. The strain and the flora thereof can be used for biological treatment of azo dye wastewater, and have the advantages of simplicity and convenience in operation, environmental friendliness, great application potential and the like.

This invention discloses a strain of *Pseudomonas nicosulfuron-methyl* capable of degrading multiple pollutants. Pseudomonas nicosulfuronedens HD1 and its applications belong to the field of functional microbial screening and application technology. This strain, originating from an aerobic composting environment of chicken manure (accession number GDMCC No:67351), can simultaneously and efficiently degrade nitropolycyclic aromatic hydrocarbons (NPH), polycyclic aromatic hydrocarbons (PAHs), and tetracycline antibiotics. In single pollutants at a concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceeded 97% after 8 hours; the degradation rate of nitrofluoranthene reached 99.01% after 48 hours; and the degradation rate of phenanthrene reached 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the degradation rate remained above 78% after 48 hours. This strain exhibits high degradation efficiency and strong environmental adaptability, providing a new microbial resource for the bioremediation of multi-contaminated sites and possessing broad application prospects.

The present invention relates to a PDRN derived from HEK293 cells and a preparation method thereof. The steps include: (1) centrifuging HEK293 cells, adding a lysate to obtain a lysate solution; (2) adding magnesium sulfate, protease and nuclease to the lysate solution, adding sodium dodecyl sulfate and sodium chloride after incubation, and obtaining a crude PDRN product after enzyme inactivation and alcohol precipitation; (3) dissolving the crude PDRN product in a sodium chloride solution, filtering, alcohol precipitation, washing, dehydration and drying to obtain PDRN. The PDRN preparation method provided by the present invention uses HEK293 cells as a source, uses a nuclease and a protease double enzyme one-pot method to prepare PDRN, has simple process control, does not have special environments such as high temperature, strong acid and alkali, and has relatively mild conditions. It does not need to add special equipment, and can be industrially produced on a large scale. In addition, the base composition ratio of the PDRN derived from HEK293 cells is completely consistent with that of the human body, has anti-inflammatory function and tissue repair activity, and has great application value in the fields of soft tissue repair, medical and aesthetic post-repair.

The present invention discloses a carbon-based adsorption / microbial aerobic degradation risk control composite material and its application, which belongs to the field of groundwater remediation technology. The composite material has a core-shell structure, including an aerobic degradation bacteria shell and a functional material core. The functional material of the core is composed of adsorption-type biochar, oxygen slow-release agent calcium peroxide and recovery-promoting factor, which are fixed by sodium alginate and calcium chloride. The aerobic shell is composed of petroleum hydrocarbon aerobic degradation bacteria Rhodococcus pyridinus, which is naturally formed on the surface of microspheres through biofilm. The material is used for aerobic bacteria to construct a local aerobic habitat in the groundwater environment, Rpf is used to improve the degradation activity of aerobic bacteria, and oxygen slow-release agents are used to overcome the inhibitory effect of groundwater oxygen deficiency on the activity of aerobic bacteria. The composite material prepared by the present invention can be used to repair groundwater contaminated by petroleum hydrocarbons, and this material has the advantages of high efficiency, long-term effect, low cost, no secondary pollution, and simple preparation method, and has the potential for large-scale promotion and application.

The present invention discloses a sorbose dehydrogenase SDH with the property of degrading vomitoxin, wherein the nucleotide sequence and amino acid sequence thereof are SEQ ID NO.1 and SEQ ID NO.2 respectively. And a directed modified enzyme SDH with improved enzyme activity F103L , whose nucleotide sequence and amino acid sequence are SEQ ID NO.3 and SEQ ID NO.4, respectively. Compared with the wild type, the mutant enzyme of the present invention has higher enzyme activity characteristics, and the enzyme can degrade deoxynivalenol (DON) into its weak toxic products. The present invention belongs to the field of agricultural biotechnology, can be applied to the preparation of DON detoxification preparations, and has broad application prospects in the field of DON detoxification.

The present application provides a biochar-based decomposition promoter, its preparation method and application. In parts by weight, the biochar-based decomposition promoter includes: 30-50 parts of biochar, 10-30 parts of organic acid potassium, 5-15 parts of nitrogen and phosphorus fertilizers, 4-20 parts of amino acids and 0.5-3 parts of spreading agents. Biochar is used as a matrix to create a microenvironment suitable for the initial attachment and reproduction of decomposing microorganisms, and the organic-inorganic nutrients provided by organic acid potassium, nitrogen and phosphorus fertilizers and amino acids are adsorbed by biochar to improve the degradation activity of microorganisms. Amino acids can be directly used by microorganisms to participate in the metabolic process, and their promoting effect on microbial diversity, quantity and functional enzyme secretion is better than that of inorganic nitrogen sources; the role of the spreading agent is to infiltrate the pores of biochar, which helps the spreading and adhesion of nutrients. The biochar-based decomposition promoter has an enhanced fertilizer retention effect, can effectively optimize the nutrient value of straw and livestock and poultry manure, and is conducive to the efficient utilization of organic resources such as straw and livestock and poultry manure.

The present application relates to the technical field of photocatalytic treatment of antibiotics in wastewater, and particularly relates to a composite photocatalytic material, a preparation method and application thereof. Melamine, palygorskite and water are mixed to obtain a mixed solution; the mixed solution is mixed with melamine foam, and after the melamine foam absorbs the mixed solution, drying is performed to obtain a precursor; the precursor is calcined to obtain the composite photocatalytic material. The composite photocatalytic material prepared by the present application can effectively improve the performance of the material in adsorbing antibiotics in water, enhance the photocatalytic degradation activity, and is easy to recycle, thus having a broad development prospect.

The invention relates to a PL35 family chondroitin sulfate lyase CHa3 as well as a coding gene and application thereof, and belongs to the technical field of enzyme engineering. The amino acid sequence of the chondroitin sulfate lyase CHa3 is shown as SEQ ID NO.2, and the chondroitin sulfate lyase CHa3 is novel chondroitin sulfate lyase, has high enzymatic activity on chondroitin sulfate, and degrades chondroitin sulfate into an incision enzyme mode; when the chondroitin sulfate polysaccharide is degraded, the final products are a series of unsaturated oligosaccharides, including but not limited to unsaturated disaccharide to unsaturated dodecanose; when the heparin is degraded, the final product is mainly unsaturated disaccharide; when dermatan sulfate is degraded, a final product comprises unsaturated tetrasaccharide to unsaturated octasaccharide. The chondroitin sulfate lyase CHa3 disclosed by the invention can be applied to preparation of CS / DS oligosaccharides and research on CS / DS structures and functions, and has a wide application prospect in the fields of medicines, foods and the like.

The invention discloses a beta-FeOOH nanorod capsule and a preparation method and application thereof.The preparation method of the beta-FeOOH nanorod capsule comprises the steps that soluble ferric salt is added into water to be completely dissolved, then a surfactant is added for a hydrolysis reaction, and beta-FeOOH nanorods are obtained; and adding the beta-FeOOH nanorod into water, uniformly dispersing the beta-FeOOH nanorod, and then adding phosphate to carry out self-assembly reaction, so as to obtain the beta-FeOOH nanorod capsule. According to the beta-FeOOH nanorod capsule and the preparation method and application thereof, the beta-FeOOH nanorod capsule with a specific morphology structure can be obtained through the preparation method, the beta-FeOOH nanostructure is enriched, and meanwhile the performance of the beta-FeOOH nanomaterial for catalytic degradation of organic pollutants can be effectively improved.

The invention discloses novel esterase as well as a coding gene and new application thereof. The amino acid sequence of the novel esterase provided by the invention is as shown in SEQ ID NO. 1, and the coding gene of the novel esterase is as shown in SEQ ID NO. 2. The novel esterase provided by the invention has excellent catalytic performance on hydrolysis of ester compounds, can be used for degrading plastic products containing plasticizers, and has a catalytic hydrolysis effect on phthalate compounds; and the method can be used for degrading phthalic acid ester compounds in food and other materials containing phthalic acid ester plasticizers.

This invention discloses a Is PETase mutants and their application in the degradation of PET plastics. This invention addresses existing... Is The PETase mutant cannot tolerate high substrate (PET plastic) concentrations, thus providing a method to improve the degradation activity of PET plastic. Is PETase mutant. For the aforementioned Is The present invention also optimizes the reaction system of the PETase mutant, thereby providing a method for depolymerizing PET plastics. Using the mutants described in this invention, such as M16-G, the degradation of high-concentration (20%) PET plastics can be completed at a Tg temperature, and it can be used for the degradation and recycling of PET plastics under industrial conditions. Furthermore, the present invention... Is PETase mutants, such as M16-G, can tolerate seawater. Seawater can be used to prepare the reaction buffer required for its degradation of PET plastics, which helps to save freshwater resources and protect the environment.

The invention discloses a method for synergistically degrading organic pollutants by using pyrite and sodium hypophosphite, and belongs to the technical field of water pollution control and advanced oxidation. The method comprises the following steps: carrying out ball milling, sieving, washing and drying on pyrite to obtain treated pyrite, and storing under an anaerobic condition; and then adding the treated pyrite and sodium hypophosphite into the water body containing the organic pollutants, and reacting under the condition of natural light to degrade the organic pollutants. Through the synergistic effect of the pyrite and the sodium hypophosphite, an exogenous oxidant does not need to be added in an organic pollutant degradation system, and free radicals can be generated only through internal reaction of the system, so that the system can still keep high degradation activity on the organic pollutants in an anaerobic or anaerobic environment; dependence of a traditional advanced oxidation technology on dissolved oxygen is broken through, the application potential of the method in in-situ remediation of underground water, bottom mud and other anoxic environments is widened, in addition, the cost is remarkably reduced, and the risk of secondary pollution is eradicated.