7results about How to "High degradation activity" patented technology

The application belongs to the technical field of enzyme engineering, and discloses a BhrPETase mutant and application thereof. The application provides a BhrPETase mutant, wherein the amino acid sequence is that threonine at the 160th position in the amino acid sequence shown in SEQ ID No. 2 is mutated to tyrosine, and the amino acid residues at other positions are unchanged. The BhrPETase mutant provided by the application has a PET degradation activity 2.32 times that of wild-type BhrPETase and 39.4% higher than that of mutant F208I under the condition that the thermal stability of the BhrPETase mutant is basically equivalent to that of wild-type BhrPETase. The BhrPETase mutant provided by the application can be applied to the fields of degrading PET, recycling PET degradation products or preparing a PET degradation agent.

This invention relates to the field of wastewater treatment technology, specifically to a microbial preparation, its preparation method, and its application, comprising: a composite microbial agent; and a calcium alginate / polyvinyl alcohol interpenetrating network aerogel carrier loaded with the composite microbial agent; the aerogel carrier contains uniformly dispersed modified nano-attapulgite clay. The microbial preparation of this invention exhibits good wastewater treatment performance and long-lasting effect.

The invention relates to an azo dye degrading bacterium SHY-2 and application thereof, the strain can efficiently degrade azo dye acid red 73 and reactive black 5, in an inorganic salt culture medium only added with glucose, cane sugar, starch or glycerol as a carbon source, the highest degradation rates of 100 mg / L acid red 73 and reactive black 5 within 5 days can reach 98.7% and 95.3% respectively, an organic nitrogen source is not needed, and the treatment cost is low. A mixed flora SH formed by the strain and a serratia bacterium also has a remarkable synergistic degradation effect on two dyes, and complete decolorization can be realized within 48-60 hours. The strain and the flora thereof can be used for biological treatment of azo dye wastewater, and have the advantages of simplicity and convenience in operation, environmental friendliness, great application potential and the like.

This invention discloses a strain of *Pseudomonas nicosulfuron-methyl* capable of degrading multiple pollutants. Pseudomonas nicosulfuronedens HD1 and its applications belong to the field of functional microbial screening and application technology. This strain, originating from an aerobic composting environment of chicken manure (accession number GDMCC No:67351), can simultaneously and efficiently degrade nitropolycyclic aromatic hydrocarbons (NPH), polycyclic aromatic hydrocarbons (PAHs), and tetracycline antibiotics. In single pollutants at a concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceeded 97% after 8 hours; the degradation rate of nitrofluoranthene reached 99.01% after 48 hours; and the degradation rate of phenanthrene reached 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the degradation rate remained above 78% after 48 hours. This strain exhibits high degradation efficiency and strong environmental adaptability, providing a new microbial resource for the bioremediation of multi-contaminated sites and possessing broad application prospects.

The present application relates to the technical field of photocatalytic treatment of antibiotics in wastewater, and particularly relates to a composite photocatalytic material, a preparation method and application thereof. Melamine, palygorskite and water are mixed to obtain a mixed solution; the mixed solution is mixed with melamine foam, and after the melamine foam absorbs the mixed solution, drying is performed to obtain a precursor; the precursor is calcined to obtain the composite photocatalytic material. The composite photocatalytic material prepared by the present application can effectively improve the performance of the material in adsorbing antibiotics in water, enhance the photocatalytic degradation activity, and is easy to recycle, thus having a broad development prospect.

This invention discloses a Is PETase mutants and their application in the degradation of PET plastics. This invention addresses existing... Is The PETase mutant cannot tolerate high substrate (PET plastic) concentrations, thus providing a method to improve the degradation activity of PET plastic. Is PETase mutant. For the aforementioned Is The present invention also optimizes the reaction system of the PETase mutant, thereby providing a method for depolymerizing PET plastics. Using the mutants described in this invention, such as M16-G, the degradation of high-concentration (20%) PET plastics can be completed at a Tg temperature, and it can be used for the degradation and recycling of PET plastics under industrial conditions. Furthermore, the present invention... Is PETase mutants, such as M16-G, can tolerate seawater. Seawater can be used to prepare the reaction buffer required for its degradation of PET plastics, which helps to save freshwater resources and protect the environment.

The invention discloses a method for synergistically degrading organic pollutants by using pyrite and sodium hypophosphite, and belongs to the technical field of water pollution control and advanced oxidation. The method comprises the following steps: carrying out ball milling, sieving, washing and drying on pyrite to obtain treated pyrite, and storing under an anaerobic condition; and then adding the treated pyrite and sodium hypophosphite into the water body containing the organic pollutants, and reacting under the condition of natural light to degrade the organic pollutants. Through the synergistic effect of the pyrite and the sodium hypophosphite, an exogenous oxidant does not need to be added in an organic pollutant degradation system, and free radicals can be generated only through internal reaction of the system, so that the system can still keep high degradation activity on the organic pollutants in an anaerobic or anaerobic environment; dependence of a traditional advanced oxidation technology on dissolved oxygen is broken through, the application potential of the method in in-situ remediation of underground water, bottom mud and other anoxic environments is widened, in addition, the cost is remarkably reduced, and the risk of secondary pollution is eradicated.