20 results about "Micrococcus luteus" patented technology

This invention belongs to the field of microbial technology, specifically relating to a strain of *Lactobacillus plantarum* that produces antimicrobial peptides and its applications. The *Lactobacillus plantarum* is specifically *Lactobacillus plantarum* (…). Lactobacillus plants Lactobacillus plantarum AF-6, with accession number CGMCC No. 33716, is a product of this invention. It generates a variety of antimicrobial peptides with broad-spectrum antibacterial activity, inhibiting spoilage bacteria such as Escherichia coli, Bacillus subtilis, Salmonella, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus. This provides a more reliable and effective option for food safety preservation.

This invention belongs to the field of wastewater treatment technology, specifically relating to a method for treating wastewater from industrialized aquaculture. This invention combines physical, biological, and chemical treatment methods, enabling efficient wastewater treatment in a short time, reducing the footprint and operating costs of treatment equipment, and exhibiting high economic efficiency and practicality. The combined use of Spirulina with Bacillus licheniformis, Bacillus subtilis, and Micrococcus luteus forms a stable algae-bacteria symbiotic system in the aquatic environment, accelerating the removal of nitrogen and phosphorus from the aquaculture wastewater and effectively reducing the chemical oxygen demand (COD). After treatment using this method, the wastewater reuse rate is no less than 80%, and the treated wastewater quality meets national discharge standards.

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The invention belongs to the technical field of sewage treatment, and particularly relates to an urban domestic sewage treatment method. Chitosan, modified activated carbon and a complex microbial inoculant formed by compounding any two strains of spirulina, rhodotorula mucilaginosa and micrococcus luteus are specifically combined, the chitosan in the composition serves as a carrier of the complex microbial inoculant, nutrients can be provided for survival of the complex microbial inoculant, and the survival time of the microbial inoculant is prolonged; the modified activated carbon has high adsorption capacity on the complex microbial inoculant, so that slow release of the complex microbial inoculant in the later period is facilitated; therefore, the composition provided by the invention can be used for purifying the urban domestic sewage, the purpose of purifying the urban domestic sewage is achieved, ecologicalization and recycling of domestic sewage treatment are realized, and a new thought is provided for urban sewage treatment.

The invention discloses an application of a bupleurum chinense extract in preparation of an antibacterial product. According to the invention, it is found for the first time that various extracts (including aqueous extracts, ethanol extracts and extraction parts of different polar solvents) of bupleurum chinense have relatively good bacteriostatic activity on streptococcus pneumoniae, methicillin-resistant staphylococcus epidermidis, micrococcus luteus and enterococcus faecium. The antibacterial spectrums and effects of different extracts are different, and the research lays a solid foundation for developing novel anti-drug-resistant bacteria drugs and natural-source antibacterial products.

This invention discloses the application of *Ulva prostrata* extract in the preparation of preventive and therapeutic antibacterial drugs. The antibacterial components of *Ulva prostrata* are extracted by ethanol extraction using a reflux extraction method. The extract shows significant antibacterial effects against *Staphylococcus aureus*, *Pseudomonas aeruginosa*, *Acinetobacter baumannii*, and *Micrococcus luteus*. The minimum inhibitory concentrations (MICs) are 3.125 mg / mL, 12.5 mg / mL, 6.25 mg / mL, and 3.125 mg / mL, respectively. The extract exhibits good stability to heat treatment and ultraviolet light, and shows good antibacterial effects in neutral and slightly alkaline environments. Metal ions affect the antibacterial effect of the extract, but the diameter of the inhibition zone is greater than 7 mm.

The invention relates to a microbial organic fertilizer and application thereof, and belongs to the technical field of biology, the microbial organic fertilizer comprises the following components: a component A comprising the following components in parts by mass: 4-6 parts of soybean peptide powder, 18-22 parts of soybean enzymatic hydrolysate, 18-24 parts of corncob furfural residue powder, 14-16 parts of mineral source humic acid, 12-18 parts of mushroom residue powder, 6-15 parts of rice hull powder, 6-9 parts of oyster shell powder, and 5-7 parts of cattle bone powder; the component B comprises a Japanese bradyrhizobium inoculant, a micrococcus luteus inoculant and a bacillus subtilis inoculant in a mass ratio of 1: (1-2): (3-6); and the mass of the component B is 1-3% of that of the component A. The bio-organic fertilizer provided by the invention can effectively prevent and treat clubroot caused by plasmodiophora brassicae and improve the yield of Chinese cabbages; the biological organic fertilizer provided by the invention can be used for effectively preventing and treating tomato bacterial wilt caused by pseudomonas solanacearum and increasing the yield of tomatoes; the bio-organic fertilizer provided by the invention can be used for effectively preventing and treating tea tree root rot caused by hard aschersonia, and the yield and the quality of tea leaves are improved.

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.

The invention relates to selective antibacterial application of micrococcus luteus to harmful staphylococcus, a composition containing the micrococcus luteus, or lysates, culture solution or extracts of the micrococcus luteus shows selective antibacterial activity to the harmful staphylococcus, especially staphylococcus aureus, methicillin-resistant staphylococcus aureus (MRSA) and staphylococcus capitis, and the composition can be used for preparing a medicine for treating the harmful staphylococcus, and can be used for preparing a medicine for treating the harmful staphylococcus, especially the staphylococcus aureus, methicillin-resistant staphylococcus aureus (MRSA) and staphylococcus capitis. Therefore, the composition can be used as an antibacterial composition in various ways, such as cosmetics, quasi-drugs, external preparations for the skin, drugs or the like.

The invention belongs to the field of medicinal chemistry, and discloses an aromatic pleuromutilin derivative with an antibacterial effect as well as a preparation method and application of the aromatic pleuromutilin derivative. The pleuromutilin is structurally modified, aromatic group modification is further increased through a reductive amination method after mercaptoethylamine is introduced into a C-14 side chain, and the structural formula of the pleuromutilin is shown in the specification. Research on an antibacterial mechanism finds that the series of derivatives can effectively interfere with expression of bacterial protein and show unique and excellent antibacterial activity, and the pleuromutilin can be applied to preparation of antibacterial agents. Compared with pleuromutilin in the prior art, most of the pleuromutilin compounds have antibacterial activity on staphylococcus aureus, escherichia coli, salmonella, micrococcus luteus and enterococcus faecalis which are common in in-vitro clinical application, hardly generate drug resistance, have stable safety and are expected to be further optimized into lead compounds of novel antibiotics.

The present invention relates to topical compositions comprising Micrococcus luteus Q24 and a viscosity modifier, the composition having a viscosity of from about 2,500,000 to about 8,000,000 cP at 25°C, and the use of these for improving the appearance of skin or at least one sign of aging, or reducing skin dryness.

The application provides a genetically engineered bacterium for producing recovery promoting factor Rpf and application thereof, relates to the technical field of bioengineering, and belongs to prokaryotes. The nucleotide sequence of the genetically engineered bacterium is shown in SEQ ID NO. 1. The sequence is obtained by codon mutation and DNA sequence optimization of a wild-type bacterium recovery promoting factor of Micrococcus luteus in an original nucleotide sequence, and the optimized nucleotide sequence is named Delta Rpf. The original nucleotide sequence is shown in SEQ ID NO. 2. The purity of the Delta Rpf recombinant protein of the application can reach 93 %, which is much higher than the purity of the protein extracted by the prior art. The enzymatic activity of the Delta Rpf recombinant protein prepared by the genetically engineered bacterium constructed by the application is significantly higher than the enzymatic activity of the unmutated Rpf protein.

The present application relates to the technical field of microorganism and new medicine and pesticide, and particularly relates to a new glycolipid active compound containing sugar, and further discloses a preparation method and application of the glycolipid active compound containing sugar.The glycolipid active compound containing sugar FW-6-2 has cytotoxic activity on staphylococcus aureus and micrococcus luteus in bacteria through activity testing, and not only a new glycolipid active compound containing sugar is found, but also a lead compound for research and development of a new bacterium inhibiting medicine is provided.

The invention discloses a strain of lactobacillus parabarium LN43 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus parabarium LN43 is GDMCC No.66615, the preservation unit is Guangdong Province Microbial Culture Collection Center, the preservation time is June 30, 2025, and the address of the preservation unit is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City. The lactobacillus parabarium LN43 has the advantages that the lactobacillus parabarium LN43 has the preservation number of GDMCC No.66615, the preservation number is GDMCC No.66615, the preservation unit is Guangdong Province, the preservation time is June 30, 2025, and the preservation unit is the June 30, 2025. The lactobacillus parabarium LN43 has relatively high acid resistance and a certain cholate tolerance. The lactobacillus parabarium LN43 has the advantages of high acid resistance and certain cholate tolerance. The strain has a very strong inhibition effect on pathogenic microorganisms such as staphylococcus aureus, salmonella pullorum, salmonella enteritidis, escherichia coli, pseudomonas aeruginosa, salmonella typhimurium, micrococcus luteus, acinetobacter baumannii and klebsiella pneumoniae, and is a broad-spectrum antibacterial microorganism.

The present invention belongs to the technical field of genetic engineering. Provided are an antimicrobial protein, and a coding gene and the use thereof. An amino acid sequence of the antimicrobial protein is a sequence as set forth in any one of SEQ ID NOs. 1-12, or a sequence with the same function as the sequence as set forth in any one of SEQ ID NOs. 1-12 after the substitution and / or deletion and / or addition of 1-30 amino acids, or a sequence with the same function as the sequence as set forth in any one of SEQ ID NOs. 1-12 after the addition of 1-20 amino acids to the C-terminus and / or N-terminus thereof. The produced antimicrobial protein provided can hydrolyze bacterial cell walls, enabling lysis of Micrococcus luteus. Upon verification, the antimicrobial protein can significantly inhibit or kill Gram-positive and Gram-negative bacteria and yeast, thereby providing a new antimicrobial protein preparation and a preparation method therefor for industries such as daily chemicals, food, and feeds.

The present invention relates to topical compositions comprising Micrococcus luteus Q24 and a viscosity modifier, the composition having a viscosity of from about 2,500,000 to about 8,000,000 cP at 25° C., and the use of these for improving the appearance of skin or at least one sign of aging, or reducing skin dryness.

The present application belongs to the technical field of biological gene engineering, and relates to a Rana chensinensis lysozyme gene, a mature polypeptide region gene, a recombinant protein and a recombinant lysozyme genetically engineered bacterium, and application of the recombinant lysozyme genetically engineered bacterium. The present application provides a Rana chensinensis lysozyme gene with a sequence shown in SEQ ID NO. 1 and a Rana chensinensis lysozyme mature polypeptide region gene with a sequence shown in SEQ ID NO. 4. After the mature polypeptide region gene is connected to a vector and transformed into Escherichia coli BL21 (DE3), a recombinant lysozyme genetically engineered bacterium for expressing a recombinant protein is obtained, and the bacteriostatic ability of the recombinant lysozyme genetically engineered bacterium on Micrococcus luteus, Staphylococcus aureus, Escherichia coli and Aeromonas hydrophila from amphibians is detected. The Rana chensinensis lysozyme provided by the present application has bacteriostatic ability on Micrococcus luteus, Aeromonas hydrophila, Escherichia coli and Staphylococcus aureus, and can be used for developing antibacterial drugs and feed additives.

The invention provides a genetically engineered bacterium for producing a resuscitation promoting factor Rpf and application of the genetically engineered bacterium, and relates to the technical field of bioengineering, the genetically engineered bacterium belongs to prokaryotes, the nucleotide sequence of the genetically engineered bacterium is as shown in SEQ ID NO.1, and the nucleotide sequence of the genetically engineered bacterium is as shown in SEQ ID NO.2. The sequence is obtained by carrying out codon mutation and DNA (Deoxyribose Nucleic Acid) sequence optimization on an original nucleotide sequence of a micrococcus luteus wild type bacterium resuscitation promoting factor, and the optimized nucleotide sequence is named as Delta Rpf; 1, and the original nucleotide sequence is as shown in SEQ ID NO. 2. The purity of the Delta Rpf recombinant protein can reach 93% and is far higher than that of protein extracted in the prior art. The activity of Delta Rpf recombinant protease prepared from the genetically engineered bacterium constructed by the invention is obviously higher than that of unmutated Rpf protease.

The present application aims to provide a eucheuma extract, a preparation method and application thereof, and the preparation method comprises the following steps: cutting eucheuma whole grass, using distilled water and 75% ethanol as extraction solvents respectively, hot reflux extraction twice, combining the filtrates, concentrating under reduced pressure, and evaporating the solvent to dryness to prepare a dry paste, and obtaining eucheuma water extract and eucheuma alcohol extract respectively. The eucheuma water extract and alcohol extract provided by the present application have significant inhibitory effects on staphylococcus aureus, escherichia coli, staphylococcus epidermidis, methicillin-resistant staphylococcus epidermidis, sarcina, bacillus, pseudomonas aeruginosa, enterococcus faecium, micrococcus luteus, acinetobacter baumannii, streptococcus pneumoniae and klebsiella pneumoniae, and have great development potential in the future research on bacteriostatic drugs, bacteriostatic cleaning products and other bacteriostatic products.