165 results about "Micrococcus luteus" patented technology

The invention discloses a harmless biological comprehensive treatment method for oily sludge. The method includes the following steps that (1), a crude oil deflocculation agent is added to deflocculate crude oil; (2), an auxiliary material is added for anaerobic compost; (3), microbial fungicide is added for aerobic fermentation; (4), plant auxiliary degradation is performed; the microbial fungicide comprises pseudomonas pseudomonas aeruginosa, micrococcus genera micrococcus luteus, bacillus bacillus subtilis, acinetobacter acinetobacter junii and acetobacter acetobacter aceti with the weight percentage being 1: 1.5: 1: 1.6: 2. The harmless biological comprehensive treatment method for the oily sludge aims to achieve the purposes of being economic, environmentally friendly and efficient.

The invention discloses a traditional Chinese medicine antibacteria composition for inhibiting micrococcus luteus, and belongs to the traditional Chinese medicine field. A rheum officinale extracting solution and water-soluble nano-silver are mixed in the antibacteria composition in proportion, two medicines are synthesized to play the synergistic effect to inhibit the micrococcus luteus. Compared with the prior art, the antibacteria composition containing the rheum officinale extract and the nano-silver can solve the problem that the dosage of the rheum officinale bacteriostatic agent in the conventional antibacteria method is great; and meanwhile, the microorganism medicine resistance generating rate is reduced by adopting two antibacterial substances in low dosage, the toxicity to the human body is lowered, and the composition is safer.

The invention discloses a stichopus japonicas BPI gene, an encoded protein, a cloning method of the stichopus japonicas BPI gene, and a method for constructing a recombinant stichopus japonicas BPI genetically engineered bacterium. The stichopus japonicas BPI gene is characterized in that a stichopus japonicas BPI gene sequence is as shown in SEQ ID NO.1; the cloning method comprises the step of designing a nested primer of RACE according to an expressed sequence tag EST sequence which is homologous to the BPI gene; a full-length gene is expanded by employing an RACE technique; a stichopus japonicas BPI protein sequence is as shown in SEQ ID NO.2; an N-terminal protein structure domain sequence is as shown in SEQ ID NO.3; an N-terminal structure domain of the stichopus japonicas BPI protein is amplified by employing primers which respectively comprise BamH I sites and Not I sites; the cloned target gene is inserted into a vector to obtain recombinant plasmids; and the recombinant plasmids are subjected to induced expression, and purification and renaturation, so as to obtain the genetically engineered bacterium. The stichopus japonicas BPI gene has the advantage of having obvious sterilization effect on vibrio parahaemolyticus, vibrio harveyi and micrococcus luteus.

The invention relates to the field of biology and in particular to an efficient repairing complex inoculant and application thereof. The complex inoculant provided by the invention comprises strains of rhodococcus, pseudomonas, saccharomyces and micrococcus luteus, where the preservation number of the rhodococcus is CGMCC No.3276; the preservation number of the pseudomonas is CGMCC No.3277; the preservation number of the saccharomyces is CGMCC No.3278; the preservation number of the micrococcus luteus is CGMCC No.3903. The complex inoculant has remarkable effects of water ecosystem restoration, water bloom prevention and deodorization, in addition, the removal rate of the complex inoculant to all organic matters reach more than 70 percent, and national policies of circular economy and energy conservation and emission reduction are met better. By using the complex inoculant, a large amount of running cost for various water areas can be saved, and recycling treatment to wastewater is achieved.

The invention belongs to the technical field of molecular biology, and particularly discloses a portunus trituberculatus mannose-binding lectin PtMBL gene, an encoding protein thereof and application.The portunus trituberculatus mannose-binding lectin PtMBL gene, the encoding protein and the application have the advantages that PtMBL gene cDNA [complementary DNA (deoxyribonucleic acid)] can be obtained from portunus trituberculatus by means of amplification by the aid of unigene and RACE technologies obtained by means of transcriptome sequencing, and obvious bacterial inhibition, bacterial binding and bacterial agglutination activity of recombinant PtMBL proteins are discovered; obvious inhibition effects can be realized by the recombinant PtMBL proteins for gram-negative bacteria (vibrioalginolyticus and pseudomonas aeruginosa) and gram-positive bacteria (staphylococcus aureus and micrococcus luteus), and the minimum inhibitory concentration is 0.81-1.61 micro-M, 0.81 micro-M, 0.81-1.61 micro-M and 3.22-6.44 micro-M; the recombinant protein PtMBL is obvious in binding activity for the vibrio alginolyticus, the pseudomonas aeruginosa, the staphylococcus aureus, the micrococcus luteus and pichia pastoris; in addition, obvious agglutination effects can be realized by the recombinant protein PtMBL for the vibrio alginolyticus, the pseudomonas aeruginosa, the staphylococcus aureus, the micrococcus luteus and the pichia pastoris in the presence of Ca2+; a foundation can be laid for controlling diseases for the portunus trituberculatus, and the portunus trituberculatus mannose-binding lectin PtMBL gene and the encoding protein have potential application values in the aspects of antibacterial medicine development, bacterial agglutination preparations, novel immunological preparations, feed additive production and the like.

The invention discloses a lonicera japonica antibacterial agent for restraining micrococcus luteus and belongs to the field of traditional Chinese medicine. The lonicera japonica antibacterial agent is characterized in that lonicera japonica extracting solution in the antimicrobial agent is mixed with water-soluble nanometer silver according to the ratio of 75:1 to 100:1, and through the combined use of the two drugs, a synergistic role in restraining the micrococcus luteus is played. Compared with the prior art, a lonicera japonica extract and a nanometer silver antibacterial agent in the lonicera japonica antibacterial agent solve the problem of higher dosage of the lonicera japonica antibacterial agent in a conventional antibacterial method; meanwhile, with the adoption of two low-dosage antibacterial substances, the occurrence probability of microorganism drug resistance is reduced, and the toxicity of the antibacterial substances to a human body is reduced, so that more safety is realized.

The invention provides a composite microbial agent and application thereof, and the composite microbial agent comprises micrococcus luteus which is preserved in Guangdong Microbial Culture Collection Center on August 12, 2021 with a preservation number of GDMCC NO: 61867; bacillus subtilis which is preserved in Guangdong Microbial Culture Collection Center on August 12, 2021 with a preservation number of GDMCC NO: 61868; and bacillus pseudomycoides which is preserved in Guangdong Microbial Culture Collection Center on June 13, 2019 with a preservation number of GDMCC NO: 60687. According to the invention, the defects in the prior art are overcome, the design is reasonable, the structure is compact, the microbial agent is easy to separate and culture, and the inventor also establishes a corresponding culture method. Researches show that the composite microbial agent has a good growth promoting effect on crops. The discovery of the strain enriches available microbial resources in China, and the strain has the advantages of stable phosphorus dissolving function, high efficiency and environmental friendliness, and has a good application prospect in the aspect of agricultural planting.