165 results about "Micrococcus luteus" patented technology

The invention relates to a method for degrading the oily sludge of a well site by using microorganisms. The method comprises the following steps of: obtaining four degrading bacteria namely pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter johnsonii from the oily sludge of the well site in the loess tableland region through culturing, separation, screening, domestication and mutation, and mixing the four degrading bacteria according to the weight ratio of 1:1:1:1 to obtain a microorganism flora for degrading the oily sludge, wherein the initial pH value equals to6, the weight ratio of carbon to nitrogen equals to 100:3, and the weight ratio of carbon to phosphorus equals to 100:0.6, the mass concentration of oils in a treatment system added with mixed bacteria is reduced from 17214 mg / kg to 1257 mg / kg within 72 h in a laboratory, and the degradation rate is 92.7%. After the oily sludge with 10.55% initial oil content is experimentally treated for 56 daysin the well site, the removal rate of oil matters can reach 89.1%, and the treatment effect is obvious.

The invention discloses a pyridine-degrading bacterium as well as a composite bacterial agent, a preparation method and uses thereof. The newly-separated Rhodococcus pyridinovora KT-J002 CGMCC No.2789 and Cellulomonas sp KT-J007 CGMCC No.2788 not only can effectually degrade the high concentration pyridine, but also has tolerance or degradation ability for toxic substances such as benzene, phenol, dimethylbenzene, quinoline, cyanide and the like; the composite bacterial agent composed of Rhodococcus pyridinovora KT-J002 CGMCC No.2789, Cellulomonas sp KT-J007 CGMCC No.2788, Paracoccus d enitrificans W12 CGMCC No.1673, Micrococcus luteus ATCC 49442 and Arthrobacter crystallopoietes ATCC 15481 is used for environmental improvement, can effectually degrade the pyridine and is particularly suitable for the biological treatment of coking waste water.

The invention discloses a solid microbial bacterium agent for petroleum degradation. The agent is prepared by mixing of the following components by weight: 85-90 parts of a microbial bacterial fertilizer, and 10-15 parts of a mixed fermentation solution of Micrococcus luteus, Bacillus cereus and Bacillus pumilus. In addition, the invention also provides a preparation method of the solid microbialbacterium agent. The solid microbial bacterium agent provided in the invention can introduce beneficial microorganisms into the soil, optimize the soil microbial system, enhance physical properties of the soil, enhance soil biological activity, and restore hardened and degraded soil, thus being an eco-environment-friendly green bacterium agent. Application of the solid microbial bacterium agent provided in the invention in the soil can promote crop growth, degrade and transform petroleum pollutants in the soil, and also can enhance the stress resistance of crops, as well as improve crop yield.

The invention belongs to the field of microorganisms and discloses a biological agent for treating ammonia-nitrogen-containing wastewater. The biological agent comprises rhodococcus ruber, micrococcus luteus, enterococcus faecalis, acinetobacter baumannii, arthrobacter crystallopoietes, thiobacillus denitrificans and pseudomonas fluorescens. The biological agent comprises multiple strains, has the advantages of reasonable compatibility and strong synergic effect and can be used for effectively removing ammonia nitrogen, sulfide, aniline substances and industrial COD in wastewater.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention belongs to the field of wastewater treatment and discloses a treatment process applicable to industrial wastewater. The treatment process applicable to the industrial wastewater comprises the following steps: 1) preparing complex microbial inoculant; 2) precipitating wastewater; 3) purifying the wastewater; 4) preparing bacterial manure. The prepared complex microbial inoculant comprises 5-6 parts of micrococcus luteus, 4-5 parts of bacillus mucilaginosus, 3-4 parts of paracoccus denitrificans, 2-3 parts of rhodococcus, 2-3 parts of acidithiobacillus ferrooxidans, 1-2 parts of bacillus megaterium and 1-2 parts of bacillus cereus. The invention also discloses bacterial manure prepared by utilizing the treatment process applicable to the industrial wastewater. The treatment process applicable to the industrial wastewater is simple and feasible, the industrial wastewater can be effectively treated, and the bacterial manure is prepared by utilizing the treatment process applicable to the industrial wastewater.

The invention relates to a starter used for producing fermented bean curd and a preparation method, the starter is composed of bacterial strains of Micrococcus luteus, Kocuria kristinae, Staphylococcus carnosus, Leuconostoc mesenteroides, bacillus subtilis and Tetragenococcus halophilus, the above bacterial strains are subjected to high density culture, concentration, freeze drying protection andfreeze drying to obtain a freeze-dried fungus powder, the freeze-dried fungus powder is mixed with the above bacterial strains according to specific proportion of 3:2:1:1:2:1 to prepare the starter product which can be directly used for producing the fermented bean curd. The technology of the method is simple, the viable bacteria amount in the obtained starter is greater than 2.0*10<11> CFU / g freeze-drying powder. The starter has the advantages of high fermentation activity, less amount and good fermentation effect, thereby the fermentation period of the fermented bean curd is shortened by 60-70 days. The starter can be liquid. The method of the invention solves the problems of unstable product quality caused by competitor pollution and decreased production capacity of bacterial strain ina traditional natural fermentation process, extra protease addition is not required in the fermentation process, the fermentation period is effectively shortened, and the flavor of the product is unique.

The invention discloses a fermentation and optimization method for tobacco flavors, which takes tobacco residual materials as raw materials, such as tobacco stems, tobacco dusts and secondary tobacco leaves, the tobacco residual materials are put into a fermentor, sugar and inorganic salt, tobacco flavor bottom materials, and water are added in to regulate pH value and a fermentation medium is obtained, and well-known preserving strain, micrococcus luteus ACCC11001, is selected as liquid fermentation strain and is fermented under certain fermentation conditions. The tobacco flavors after fermentation culture not only can maintain characteristic fragrance contained in original flavors, but also can ensure auxiliary fragrance obtained by fermentation to combine perfectly with the characteristic fragrance, and compared with the original flavors, the grade of the fragrance is improved obviously and the fragrance smells more elegant. The production process of the invention is simple, convenient and easy for extension, thus being provided with greater development and application value.

The invention discloses a harmless biological comprehensive treatment method for oily sludge. The method includes the following steps that (1), a crude oil deflocculation agent is added to deflocculate crude oil; (2), an auxiliary material is added for anaerobic compost; (3), microbial fungicide is added for aerobic fermentation; (4), plant auxiliary degradation is performed; the microbial fungicide comprises pseudomonas pseudomonas aeruginosa, micrococcus genera micrococcus luteus, bacillus bacillus subtilis, acinetobacter acinetobacter junii and acetobacter acetobacter aceti with the weight percentage being 1: 1.5: 1: 1.6: 2. The harmless biological comprehensive treatment method for the oily sludge aims to achieve the purposes of being economic, environmentally friendly and efficient.

The invention discloses a stichopus japonicas BPI gene, an encoded protein, a cloning method of the stichopus japonicas BPI gene, and a method for constructing a recombinant stichopus japonicas BPI genetically engineered bacterium. The stichopus japonicas BPI gene is characterized in that a stichopus japonicas BPI gene sequence is as shown in SEQ ID NO.1; the cloning method comprises the step of designing a nested primer of RACE according to an expressed sequence tag EST sequence which is homologous to the BPI gene; a full-length gene is expanded by employing an RACE technique; a stichopus japonicas BPI protein sequence is as shown in SEQ ID NO.2; an N-terminal protein structure domain sequence is as shown in SEQ ID NO.3; an N-terminal structure domain of the stichopus japonicas BPI protein is amplified by employing primers which respectively comprise BamH I sites and Not I sites; the cloned target gene is inserted into a vector to obtain recombinant plasmids; and the recombinant plasmids are subjected to induced expression, and purification and renaturation, so as to obtain the genetically engineered bacterium. The stichopus japonicas BPI gene has the advantage of having obvious sterilization effect on vibrio parahaemolyticus, vibrio harveyi and micrococcus luteus.

The invention relates to the field of biology and in particular to an efficient repairing complex inoculant and application thereof. The complex inoculant provided by the invention comprises strains of rhodococcus, pseudomonas, saccharomyces and micrococcus luteus, where the preservation number of the rhodococcus is CGMCC No.3276; the preservation number of the pseudomonas is CGMCC No.3277; the preservation number of the saccharomyces is CGMCC No.3278; the preservation number of the micrococcus luteus is CGMCC No.3903. The complex inoculant has remarkable effects of water ecosystem restoration, water bloom prevention and deodorization, in addition, the removal rate of the complex inoculant to all organic matters reach more than 70 percent, and national policies of circular economy and energy conservation and emission reduction are met better. By using the complex inoculant, a large amount of running cost for various water areas can be saved, and recycling treatment to wastewater is achieved.

This invention relates to Micrococcus luteus containing compositions useful for controlling skin disorders in which bacteria are a causative component. The invention also provides for a new strain of Micrococcus luteus useful in these compositions.

The invention relates to a Pediococcus acidilactici strain which is named Pediococcus acidilactici AAF 3-3. The Pediococcus acidilactici AAF 3-3 is classified as Pediococcus acidilactici, and is preserved under the preservation number of CGMCC No. 17856 since the preservation date of May 27, 2019 in the preservation unit of China General Microbiological Culture Collection Center (No.3, Court 1, West Beichen Road, Chaoyang District, Beijing). The Pediococcus acidilactici AAF 3-3 disclosed by the invention has high tolerance to main stress pressures (acetic acid, lactic acid, high temperature and ethanol) and an extreme pH value during solid-state fermentation of edible vinegar, and has an L-lactic acid production function; and moreover, the Pediococcus acidilactici AAF 3-3 has significant inhibitory effects on escherichia coli, micrococcus luteus and staphylococcus aureus as well as resistance to gentamicin, chloramphenicol, erythromycin, ampicillin and kanamycin. Thus, the Pediococcusacidilactici AAF 3-3 has application potential in the field of foods.

The invention relates to a natural bacteriostat composition applied to synergistic inhibition to common microbes. The composition of epsilon-polylysine and nisin according to different proportions realizes the synergistic function on inhibition of escherichia coli, micrococcus luteus, bacillus subtilis, staphylococcus aureus and aspergillus niger, and is also applied to the freshness preservation of fresh-cut carrots. According to the natural bacteriostat composition applied to the synergistic inhibition to the common microbes, which is disclosed by the invention, the problem that the use amount of single bacteriostat is relatively large in the conventional bacteriostasis method is solved by the composition of the epsilon-polylysine and the nisin, the antibacterial spectrum is expanded by simultaneously utilizing the composition of the two bacteriostat, and the natural bacteriostat composition disclosed by the invention can be applied to food preservation under the condition of lower dosage and is high in safety.

The invention discloses a micrococcus luteus 4006 with a collection number of CGMCC No.6072 (China General Microbiological Culture Collection Center Number 6072). In addition, the invention further discloses application of the micrococcus luteus in degradation of petroleum pollutants. The micrococcus luteus originates from soil, so that the micrococcus luteus quickly becomes a dominant populationand takes up a certain ecological niche after entering the soil, so as to effectively promote degradation of petroleum hydrocarbons, optimize soil microorganism systems, improve soil physical characteristics, improve soil biological activity and repair hardened and degraded soil. The micrococcus luteus is a dominant strain screened by taking raw oil as an unique carbon source, can change the property of the raw oil in the growth and propagation process to change the components of the raw oil so as to take effects of emulsifying, wetting and dispersing the raw oil, and can degrade the petroleum pollutants in the soil in a short time.

The invention relates to the technical field of molecular biology, in particular to eriocheir sinensis Crustin-1 gene clone and a recombination expression technology thereof. Crustin-1 gene of which the cDNA span is 821bp is cloned from eriocheir sinensis by using an expressed sequence tag (EST) technology and a 3' and 5' end rapid augmentation technology (RACE) and comprises a 315bp open reading frame, polyadenylic acid tailing signals and polyadenylic acid tails, 104 amino acids are coded, the length of a 5' non-coding region is 239bp, the length of a 3' non-coding region is 267bp, and the Crustin-1gene play an important role in the immune defense aspect of the eriocheir sinensis. The invention obtains eriocheir sinensis Crustin-1 protein by using the in-vitro recombination expression technology, the recombination protein has stronger bacteriostatic activity for gram-positive bacteria, the minimal inhibitory concentrations of the recombination protein for micrococcus luteus, bacillus subtilis, micrococcus tetragenus and bacillus thuringiensis are 0.12 mu m, 0.23 mu m, 0.46 mu m and 0.12 mu m respectively, and the recombination protein does not have obvious inhibiting function. The invention can lay the foundation for the disease control of the eriocheir sinensis, the gene assistant breeding and the development of feed additives.

The invention discloses a preparation method of micrococcus luteus immobilized beads. The method comprises the following steps: burying micrococcus luteus into a sodium alginate-porous soil filtering material mixed solution, so as to stably keep the activity of the micrococcus luteus; enabling the micrococcus luteus to grow into a source for providing resuscitation-promoting factor Rpf proteins for a long period, wherein the Rpf proteins can resuscitate and promote a potential functional bacterial group in a toxic and difficult-to-degrade organic pollution environment, so as to enhance efficient degradation of pollutants. The invention further provides application of the micrococcus luteus immobilized beads in the toxic organic pollutants; compared with common immobilized carrier powder active carbon, the operation cost is lower, and the micrococcus luteus immobilized beads have more remarkable economic benefits and environment-friendly benefits. The micrococcus luteus immobilized beads can be used for a biological treatment system of toxic and harmful, difficult-to-degrade and high-concentration organic industrial wastewater including coking wastewater, high-salt phenol-containing wastewater, printing and drying wastewater, pharmaceutical wastewater and the like.

The invention relates to the aplication of amigo-smile polyoses used for resisting bacteria or viruses, in particular to the application of the amigo-smile polyoses for preparing a medicament with the effects of resisting the bacteria or the viruses. The amigo-smile polyoses of the invention has inhibition effects to a plurality of bacteria or viruses, such as Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, salmonella, Proteus vuigaris, Shigella dysenteriae, Candida albicans or parainfluenza virus, and the like. The known method of the prior art can be adopted to prepare the amigo-smile polyoses of the invention into a medicament compound and the medicament compounds of any preparation formulation acceptable on pharmacy, such as granular formulation, oral liquid, capsule, troche, injection, pulvis, and the like.

The invention belongs to the technical field of molecular biology, and particularly discloses a portunus trituberculatus mannose-binding lectin PtMBL gene, an encoding protein thereof and application.The portunus trituberculatus mannose-binding lectin PtMBL gene, the encoding protein and the application have the advantages that PtMBL gene cDNA [complementary DNA (deoxyribonucleic acid)] can be obtained from portunus trituberculatus by means of amplification by the aid of unigene and RACE technologies obtained by means of transcriptome sequencing, and obvious bacterial inhibition, bacterial binding and bacterial agglutination activity of recombinant PtMBL proteins are discovered; obvious inhibition effects can be realized by the recombinant PtMBL proteins for gram-negative bacteria (vibrioalginolyticus and pseudomonas aeruginosa) and gram-positive bacteria (staphylococcus aureus and micrococcus luteus), and the minimum inhibitory concentration is 0.81-1.61 micro-M, 0.81 micro-M, 0.81-1.61 micro-M and 3.22-6.44 micro-M; the recombinant protein PtMBL is obvious in binding activity for the vibrio alginolyticus, the pseudomonas aeruginosa, the staphylococcus aureus, the micrococcus luteus and pichia pastoris; in addition, obvious agglutination effects can be realized by the recombinant protein PtMBL for the vibrio alginolyticus, the pseudomonas aeruginosa, the staphylococcus aureus, the micrococcus luteus and the pichia pastoris in the presence of Ca2+; a foundation can be laid for controlling diseases for the portunus trituberculatus, and the portunus trituberculatus mannose-binding lectin PtMBL gene and the encoding protein have potential application values in the aspects of antibacterial medicine development, bacterial agglutination preparations, novel immunological preparations, feed additive production and the like.

The invention discloses a preparation method of an acidithiobacillus ferrooxidian genetic engineering strain with quick growth speed and strong uranium and heat resistance capacities as well as the application thereof, wherein the strain is Acidithiobacillus ferrooxidans QEY-6(pJRD215-PT-rcs), CCTCCM 209156; and the preparation method comprises the following steps: firstly, augmenting a T5 promoter and a T0 terminator of pQE30, and establishing a shuttle expression vector pJRD215-PT together with pJRD 215; secondly, augmenting and cloning a micrococcus luteus rpf gene, a caulobacter crescentuscc3302 gene and a sulfolobus shibatae ss10b gene to a T vector; thirdly, establishing a recombined shuttle expression vector pJRD215-PT-rcs; and fourthly, establishing the acidithiobacillus ferrooxidian genetic engineering strain which fusedly expresses rpf, cc3302 and ssh10b genes. The invention has simple operation and good repeatability, and can shorten the uranium leaching period, improve theleaching rate and reduce the acid-consuming rate.

The invention provides a Peptocin antibacterial peptide separated from heterometrus spinifer and obtained through a chemical synthesis method. The antibacterial peptide has a remarkable antibacterial effect on clinically-separated multi-drug resistant acinetobacter baumannii, and can be used for efficiently inhibiting the growth of the drug resistant acinetobacter baumannii; and meanwhile, human red blood cell toxicity tests also show that the antibacterial peptide is small in toxicity and can be applied to the preparation of drugs for treating or preventing diseases caused by the drug resistant acinetobacter baumannii and particularly to the preparation of drugs for treating serious infectious diseases such as ventilator-associated pneumonia, septicemia, meningitis, otitis media, skin soft-tissue infection, urinary system infection, central nervous system infection and the like caused by the drug resistant acinetobacter baumannii. The antibacterial peptide provided by the invention is remarkable in inhibiting effect on gram bacteria, unique in antibacterial mechanism and low in toxicity, and can also be applied to the preparation of drugs for treating or preventing diseases caused by micrococcus luteus, salmonella and other gram bacteria.

The invention relates to the field of microorganism screening and culturing, in particular to a resuscitation medium capable of promoting growth of VBNC (viable but non-culture) bacteria and improving separation abundance, and a preparation method and application thereof. The resuscitation medium capable of promoting growth of VBNC bacteria and improving separation abundance is composed of medium base fluid, bacterium fluid and soil fluid, the bacterium fluid is prepared by culturing micrococcus luteus on culturing fluid medium to the later period of log phase, contains resuscitation promoting factors Rpf after culturing fluid medium is removed by centrifuging to extract protein and is 0.5-30% of the medium base fluid according to the volume ratio, and the soil fluid is prepared by adding water to the soil which is dried, screened and subjected to impurity removal, sterilizing multiple times and filtering to obtain filtrate or centrifuging to obtain supernatant, and is 0.1-20% of the medium base fluid according to the volume ratio. By means of the resuscitation promoting factors Rpf and by adding the soil fluid to prepare the resuscitation medium, resuscitation of the VBNC bacteria can be promoted further, and abundance of separating the VBNC bacteria from the ecological environment is improved. The resuscitation medium and the preparation method thereof have the advantages of simplicity in operation and fast resuscitation.

The invention discloses a bacteriostatic agent, and belongs to the field of traditional Chinese medicine. Honeysuckle flower extract and water-solubility nano-silver in the bacteriostatic agent are mixed at the proportion of (75:1)-(100:1), and are combined to play a synergetic effect for restraining escherichia coli O157:H7 and micrococcus luteus. Compared with the prior art, the honeysuckle flower extract and the nano-silver bacteriostatic agent solve the problem that in the conventional bacteriostatic method, the honeysuckle flower bacteriostatic agent dosage is great; meanwhile, the drug resistant generation probability of organisms by adopting two bacteriostatic substances with low dosage is reduced, the toxicity to human bodies is lowered, and the bacteriostatic agent is safe.

The invention belongs to the technical field of molecular biology and particularly relates to a portunus trituberculatus PtCrustin-2 gene, and coded protein and application thereof. The PtCrustin-2 gene DNA is amplified from portunus trituberculatus by utilizing a cDNA library and an RACE technology and the gene exerts important effect in the aspect of immunologic defence of the portunus trituberculatus. The recombinant PtCrustin-2 protein has obvious bacteriostatic activity on Gram negative bacteria and Gram positive bacteria. The minimum inhibitory concentrations of the gene on vibrio alginolyticus, pseudomonas aeruginosa, staphylococcus aureus and micrococcus luteus are 24.75 MuM, 12.37 MuM, 49.51 MuM and 49.51 MuM respectively. The gene does not have obvious bacteriostatic effect on fungi pichia pastoris. Basis is laid for diseases prevention and treatment on the portunus trituberculatus, gene auxiliary breeding and development of feed additives.

The invention discloses a method for treating sewage by using microorganisms. The method comprises the following steps: pulverizing chestnut shells and camellia oleifera shells, adding phosphoric acid, activating, and carbonizing to obtain an adsorbent; respectively crushing pomacea canaliculata, the oil tea cake and the sugarcane filter mud, adding into a stirring tank and adding molasses, peptone and water to prepare a microbial nitrogen source; mixing micrococcus luteus, frankia, nitrite oxidizing bacteria, coriolis palustris, bacillus subtilis and EM bacteria to obtain a compound bacteriasolution; uniformly mixing chitosan, sodium alginate, talcum powder and redispersible latex powder to prepare a biological carrier; and uniformly mixing the composite bacterial liquid and the microbial nitrogen source, culturing, adding the adsorbent and the biological carrier, uniformly stirring, and drying to obtain the microbial sewage treatment agent. The microbial treatment agent prepared bythe method can effectively remove ammonia nitrogen, heavy metal ions, COD and the like in sewage and is relatively low in investment cost and has a very good application prospect in sewage treatment.

The invention discloses a preparation method of a composite microbial inoculant for sludge treatment. The method comprises the following steps: respectively culturing Paenibacillus, Rhodococcus, Pseudomonas putida, Micrococcus luteus and Acinetobacter baumannii to obtain a Paenibacillus solution, a Rhodococcus solution, a Pseudomonas putida solution, a Micrococcus luteus solution and an Acinetobacter baumannii solution; mixing the Paenibacillus solution, Rhodococcus solution, Pseudomonas putida solution, Micrococcus luteus solution and Acinetobacter baumannii solution to obtain a composite bacterium solution; and putting the composite bacterium solution into a composite bacterium culture medium, and carrying out mixed culture fermentation to obtain the composite microbial inoculant for degrading zearalenone. The composite microbial inoculant can efficiently degrade zearalenone, and has the advantages of low cost and no secondary pollution.

The invention provides a composite microbial agent and application thereof, and the composite microbial agent comprises micrococcus luteus which is preserved in Guangdong Microbial Culture Collection Center on August 12, 2021 with a preservation number of GDMCC NO: 61867; bacillus subtilis which is preserved in Guangdong Microbial Culture Collection Center on August 12, 2021 with a preservation number of GDMCC NO: 61868; and bacillus pseudomycoides which is preserved in Guangdong Microbial Culture Collection Center on June 13, 2019 with a preservation number of GDMCC NO: 60687. According to the invention, the defects in the prior art are overcome, the design is reasonable, the structure is compact, the microbial agent is easy to separate and culture, and the inventor also establishes a corresponding culture method. Researches show that the composite microbial agent has a good growth promoting effect on crops. The discovery of the strain enriches available microbial resources in China, and the strain has the advantages of stable phosphorus dissolving function, high efficiency and environmental friendliness, and has a good application prospect in the aspect of agricultural planting.