36 results about "Micrococcus luteus" patented technology

This invention belongs to the field of microbial technology, specifically relating to a strain of *Lactobacillus plantarum* that produces antimicrobial peptides and its applications. The *Lactobacillus plantarum* is specifically *Lactobacillus plantarum* (…). Lactobacillus plants Lactobacillus plantarum AF-6, with accession number CGMCC No. 33716, is a product of this invention. It generates a variety of antimicrobial peptides with broad-spectrum antibacterial activity, inhibiting spoilage bacteria such as Escherichia coli, Bacillus subtilis, Salmonella, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus. This provides a more reliable and effective option for food safety preservation.

The invention provides a microbial agent for overcoming successive cropping obstacles of beet and a preparation method and application thereof, and belongs to the technical field of microbial agents. The microbial agent comprises the following components in parts by mass: 30-40 parts of sodium alginate, 60-70 parts of a bacterial liquid, 3-5 parts of charcoal, 2-4 parts of calcium chloride and 10-20 parts of a soil conditioner, the bacterial liquid comprises a micrococcus luteus GZU-Mi02 bacterial liquid, a fragrance-like fungus C40 bacterial liquid and a bacillus amyloliquefaciens Z-2 bacterial liquid. The microbial agent prepared by the invention not only can solve the successive cropping obstacle of beet, but also can promote growth of beet and increase income, and meanwhile, improves soil nutrients and structure, thereby fundamentally eliminating the successive cropping obstacle of beet.

This invention belongs to the field of wastewater treatment technology, specifically relating to a method for treating wastewater from industrialized aquaculture. This invention combines physical, biological, and chemical treatment methods, enabling efficient wastewater treatment in a short time, reducing the footprint and operating costs of treatment equipment, and exhibiting high economic efficiency and practicality. The combined use of Spirulina with Bacillus licheniformis, Bacillus subtilis, and Micrococcus luteus forms a stable algae-bacteria symbiotic system in the aquatic environment, accelerating the removal of nitrogen and phosphorus from the aquaculture wastewater and effectively reducing the chemical oxygen demand (COD). After treatment using this method, the wastewater reuse rate is no less than 80%, and the treated wastewater quality meets national discharge standards.

The present application relates to the field of pharmaceutical chemistry, and specifically discloses a emodin mannich base, a preparation method and application thereof. The emodin mannich base has a structure as shown in formula I, the structure is simple, has strong in-vitro antimicrobial activity, especially high inhibitory activity to staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteus vulgaris, pseudomonas aeruginosa, shigella dysenteriae, salmonella typhimurium and other bacteria, and candida utilis, candida albicans, saccharomyces cerevisiae, monascus and other fungi, and can be used for preparing antibacterial and / or antifungal drugs.

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The invention discloses the application and method of Leuconostoc mesenteroides in the preparation of fermented food or beverage, and relates to the field of microbial fermentation technology. Leuconostoc mesenteroides I1 / 53 produces antibacterial components such as hydrophilic esters, thioaminophenol C, cerulenin and faecalis in fermented sugarcane juice beverage, which can effectively inhibit foodborne pathogens, including Staphylococcus aureus, Escherichia coli, Salmonella, Listeria monocytogenes, Staphylococcus lentus, Bacillus subtilis, Bacillus cereus and Micrococcus luteus; at the same time, it can also enhance the flavor characteristics of sugarcane juice beverage.

The invention belongs to the technical field of sewage treatment, and particularly relates to an urban domestic sewage treatment method. Chitosan, modified activated carbon and a complex microbial inoculant formed by compounding any two strains of spirulina, rhodotorula mucilaginosa and micrococcus luteus are specifically combined, the chitosan in the composition serves as a carrier of the complex microbial inoculant, nutrients can be provided for survival of the complex microbial inoculant, and the survival time of the microbial inoculant is prolonged; the modified activated carbon has high adsorption capacity on the complex microbial inoculant, so that slow release of the complex microbial inoculant in the later period is facilitated; therefore, the composition provided by the invention can be used for purifying the urban domestic sewage, the purpose of purifying the urban domestic sewage is achieved, ecologicalization and recycling of domestic sewage treatment are realized, and a new thought is provided for urban sewage treatment.

In one aspect, the invention relates to compounds useful as hybrid antibiotics, acting as both activators of the ClpP protease and inhibitors of RNA polymerase activity. Also disclosed are methods of using the disclosed compounds in the treatment of infectious diseases such as, for example, biofilm-mediated diseases and infectious diseases due to prosthetic joint infections, intracellular bacteria, and Gram-positive bacteria (e.g., Gram-positive bacteria selected from Bacillus anthracis, Bacillus cereus, Bacillus subtilis, Clostridium difficile, Clostridium tetani, Clostridium botulinum, Clostridium perfringens, Corynebacterium diphtheria, Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, Listeria ivanovii, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus hyicus, Staphylococcus intermedius, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus agalactiae). This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

The present invention relates to the field of biotechnology, and in particular to a Chinese horseshoe crab TaTrs_ALF_α1 antimicrobial peptide and a preparation method and application thereof. The present invention studies a horseshoe crab antimicrobial peptide: TaTrs_ALF_α1 for Chinese horseshoe crabs. It has been verified that the antimicrobial peptide has good inhibitory effects on common pathogenic bacteria in aquaculture: Staphylococcus aureus, Micrococcus luteus, Aeromonas hydrophila and / or Pseudomonas stutzeri. At the same time, it also has a good growth inhibition effect on gastric cancer cells SGC-7901 and / or esophageal cancer cells Eca-109. When used as a drug for treating esophageal cancer, the inhibitory effect on esophageal cancer cells Eca-109 can reach more than 90% when the concentration reaches 80 μg / mL, and the anticancer effect is good. Therefore, the antimicrobial peptide of the present application can be widely used in the fields of drug research and development and animal breeding immunoenhancers, and is a rare antimicrobial peptide.

The invention discloses an application of a bupleurum chinense extract in preparation of an antibacterial product. According to the invention, it is found for the first time that various extracts (including aqueous extracts, ethanol extracts and extraction parts of different polar solvents) of bupleurum chinense have relatively good bacteriostatic activity on streptococcus pneumoniae, methicillin-resistant staphylococcus epidermidis, micrococcus luteus and enterococcus faecium. The antibacterial spectrums and effects of different extracts are different, and the research lays a solid foundation for developing novel anti-drug-resistant bacteria drugs and natural-source antibacterial products.

This invention discloses the application of *Ulva prostrata* extract in the preparation of preventive and therapeutic antibacterial drugs. The antibacterial components of *Ulva prostrata* are extracted by ethanol extraction using a reflux extraction method. The extract shows significant antibacterial effects against *Staphylococcus aureus*, *Pseudomonas aeruginosa*, *Acinetobacter baumannii*, and *Micrococcus luteus*. The minimum inhibitory concentrations (MICs) are 3.125 mg / mL, 12.5 mg / mL, 6.25 mg / mL, and 3.125 mg / mL, respectively. The extract exhibits good stability to heat treatment and ultraviolet light, and shows good antibacterial effects in neutral and slightly alkaline environments. Metal ions affect the antibacterial effect of the extract, but the diameter of the inhibition zone is greater than 7 mm.

The invention relates to the technical field of essential oil, in particular to peach leaf essential oil and application thereof. According to the method, the peach leaf essential oil is extracted by adopting a water distillation method, the components of the peach leaf essential oil are identified, 40 volatile chemical components are identified by utilizing headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GCMS), 23 chemical components are identified by utilizing ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS), and the content of the volatile chemical components in the peach leaf essential oil is greatly improved. Researches find that the peach leaf essential oil can effectively inhibit and kill escherichia coli, staphylococcus aureus, micrococcus luteus, pantoea, fusarium oxysporum and monilinia fusarium, and in addition, the invention also finds that the peach leaf essential oil has better oxidation resistance and free radical scavenging ability, so that the invention develops new application of the peach leaf essential oil.

The invention relates to a microbial organic fertilizer and application thereof, and belongs to the technical field of biology, the microbial organic fertilizer comprises the following components: a component A comprising the following components in parts by mass: 4-6 parts of soybean peptide powder, 18-22 parts of soybean enzymatic hydrolysate, 18-24 parts of corncob furfural residue powder, 14-16 parts of mineral source humic acid, 12-18 parts of mushroom residue powder, 6-15 parts of rice hull powder, 6-9 parts of oyster shell powder, and 5-7 parts of cattle bone powder; the component B comprises a Japanese bradyrhizobium inoculant, a micrococcus luteus inoculant and a bacillus subtilis inoculant in a mass ratio of 1: (1-2): (3-6); and the mass of the component B is 1-3% of that of the component A. The bio-organic fertilizer provided by the invention can effectively prevent and treat clubroot caused by plasmodiophora brassicae and improve the yield of Chinese cabbages; the biological organic fertilizer provided by the invention can be used for effectively preventing and treating tomato bacterial wilt caused by pseudomonas solanacearum and increasing the yield of tomatoes; the bio-organic fertilizer provided by the invention can be used for effectively preventing and treating tea tree root rot caused by hard aschersonia, and the yield and the quality of tea leaves are improved.

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.

The invention relates to selective antibacterial application of micrococcus luteus to harmful staphylococcus, a composition containing the micrococcus luteus, or lysates, culture solution or extracts of the micrococcus luteus shows selective antibacterial activity to the harmful staphylococcus, especially staphylococcus aureus, methicillin-resistant staphylococcus aureus (MRSA) and staphylococcus capitis, and the composition can be used for preparing a medicine for treating the harmful staphylococcus, and can be used for preparing a medicine for treating the harmful staphylococcus, especially the staphylococcus aureus, methicillin-resistant staphylococcus aureus (MRSA) and staphylococcus capitis. Therefore, the composition can be used as an antibacterial composition in various ways, such as cosmetics, quasi-drugs, external preparations for the skin, drugs or the like.

Disclosed are novel bioremediation formulations and inocula for formulation of bioremediation formulations. The formulations of the present disclosure comprise viable microorganisms of the species Paenibacillus xylanexedens, Pseudomonas fluorescens, Bacillus safensis, Streptomyces griseus, Micrococcus luteus, and Bacillus megaterium and can be used to degrade glyphosate residue associated with a substrate contaminated with glyphosate, and thereby remedially treat the substrate. Related methods are also disclosed.

The invention belongs to the field of medicinal chemistry, and discloses an aromatic pleuromutilin derivative with an antibacterial effect as well as a preparation method and application of the aromatic pleuromutilin derivative. The pleuromutilin is structurally modified, aromatic group modification is further increased through a reductive amination method after mercaptoethylamine is introduced into a C-14 side chain, and the structural formula of the pleuromutilin is shown in the specification. Research on an antibacterial mechanism finds that the series of derivatives can effectively interfere with expression of bacterial protein and show unique and excellent antibacterial activity, and the pleuromutilin can be applied to preparation of antibacterial agents. Compared with pleuromutilin in the prior art, most of the pleuromutilin compounds have antibacterial activity on staphylococcus aureus, escherichia coli, salmonella, micrococcus luteus and enterococcus faecalis which are common in in-vitro clinical application, hardly generate drug resistance, have stable safety and are expected to be further optimized into lead compounds of novel antibiotics.

The present invention relates to topical compositions comprising Micrococcus luteus Q24 and a viscosity modifier, the composition having a viscosity of from about 2,500,000 to about 8,000,000 cP at 25°C, and the use of these for improving the appearance of skin or at least one sign of aging, or reducing skin dryness.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum for producing antibacterial peptide and application of the lactobacillus plantarum. The lactobacillus plantarum is specifically lactobacillus plantarum AF-6, and the preservation number of the lactobacillus plantarum AF-6 is CGMCC (China General Microbiological Culture Collection Center) No. 33716. The lactobacillus plantarum AF-6 disclosed by the invention can generate various antibacterial peptides, has broad-spectrum antibacterial activity, can play a role in inhibiting putrefying bacteria such as escherichia coli, bacillus subtilis, salmonella, micrococcus luteus, pseudomonas aeruginosa, bacillus cereus and staphylococcus aureus, and can provide a more stable and effective choice for safety and preservation of food industry.

The application provides a genetically engineered bacterium for producing recovery promoting factor Rpf and application thereof, relates to the technical field of bioengineering, and belongs to prokaryotes. The nucleotide sequence of the genetically engineered bacterium is shown in SEQ ID NO. 1. The sequence is obtained by codon mutation and DNA sequence optimization of a wild-type bacterium recovery promoting factor of Micrococcus luteus in an original nucleotide sequence, and the optimized nucleotide sequence is named Delta Rpf. The original nucleotide sequence is shown in SEQ ID NO. 2. The purity of the Delta Rpf recombinant protein of the application can reach 93 %, which is much higher than the purity of the protein extracted by the prior art. The enzymatic activity of the Delta Rpf recombinant protein prepared by the genetically engineered bacterium constructed by the application is significantly higher than the enzymatic activity of the unmutated Rpf protein.

The invention provides a preparation method of a polypeptide product containing osteogenic growth peptide, which comprises the following steps: sterilizing bone marrow or blood of an animal as a raw material; after sterilization, adjusting the pH value to 6-8, inoculating 1-3% of micrococcus luteus (1-8 * 10 < 9 > cfu / mL), inoculating 2-5% of lactobacillus curvatus (1-8 * 10 < 10 > cfu / mL), inoculating 0.5-4% of bacillus coagulans (1-8 * 10 < 8 > cfu / g), and fermenting at 30-42 DEG C for 2-6 hours; adjusting the temperature to 20-30 DEG C and the pH value to 6-8, inoculating 1-8% of geotrichum candidum (1-8 * 10 < 6 > cfu / mL), fermenting for 1-5 days, and sterilizing for 20-40 minutes under the microwave of 200-500 W to obtain fermentation liquor; adjusting the pH value of the fermentation liquor to 5-9, adding 0.5-5% of kiwi fruit protease, bromelain and alkaline protease according to a ratio of 1: (1-4): (2-5), carrying out enzymolysis at 45-65 DEG C for 2-8 hours, heating to 85-95 DEG C, and carrying out heat preservation for 20-40 minutes to obtain enzymatic hydrolysate; and separating from the enzymatic hydrolysate to obtain the polypeptide product containing the osteogenic growth peptide. The invention provides a brand-new method for preparing the polypeptide product containing the osteogenic growth peptide, the process is relatively simple, and the yield of the osteogenic growth peptide is relatively high, so that the cost is reduced.

The invention relates to a plant composition fermentation product as well as a preparation method and application thereof. The plant composition fermentation product is a product obtained by mixing and fermenting an extracting solution of a plant composition and a microbial agent, the plant composition comprises phyllanthus emblica, lophatherum gracile, borneol and coptis chinensis; bacteria in the microbial agent comprise any one or a combination of at least two of streptococcus thermophilus, bifidobacterium or micrococcus luteus. According to the plant composition provided by the invention, on the basis of the theory of traditional Chinese medicine, the principle of monarch, minister, assistant and guide is applied, the four plant components have synergistic interaction, and meanwhile, the plant composition leavening obtained by further fermenting the extracting solution of the plant composition not only improves the quality of the plant composition from three aspects of taste, color and smell, but also improves the quality of the plant composition. The enrichment of bacteriostatic active substances is also realized, so that the toothpaste has a more excellent effect of relieving tooth sensitivity.

The invention provides a berry rooting agent as well as a preparation method and application thereof, and belongs to the technical field of plant growth promoters. The rooting agent comprises indolebutyric acid, chrysin, thymol, methionine, cane sugar, a toona sinensis leaf extract and a compound microbial agent. The compound microbial agent is a mixture of micrococcus luteus and lactobacillus paracasei L1. Compared with a traditional plant rooting agent, the rooting agent has the advantages that the components are reasonably proportioned, so that the rooting of plants with shallow root systems can be effectively promoted. Hormones generated in the microbial metabolism process promote dedifferentiation of plant cells, callus is generated, and growth of adventitious roots of cuttings is promoted. Through the synergistic effect of multiple biological mechanisms, the effects of removing pathogenic bacteria in soil and protecting plant root systems are achieved. The berry rooting agent provided by the invention can also provide sufficient nutrient substances for rapid rooting of plants, and is suitable for agricultural popularization and application.

The present application relates to the technical field of microorganism and new medicine and pesticide, and particularly relates to a new glycolipid active compound containing sugar, and further discloses a preparation method and application of the glycolipid active compound containing sugar.The glycolipid active compound containing sugar FW-6-2 has cytotoxic activity on staphylococcus aureus and micrococcus luteus in bacteria through activity testing, and not only a new glycolipid active compound containing sugar is found, but also a lead compound for research and development of a new bacterium inhibiting medicine is provided.

The invention relates to the technical field of microorganisms, and discloses bacillus velezensis inducing bacteria and application thereof in lipopeptide production, the bacillus velezensis is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 6, 2022, and the preservation number is CGMCC No.24640. The invention further discloses a preparation method of the bacillus velezensis inducing bacteria. According to the invention, the bacillus velezensis is taken as an original strain, and a mutant strain with high yield of lipopeptide is successfully screened out through the technical means of ultraviolet mutagenesis, preliminary screening, secondary screening and the like, so that the yield of lipopeptide is remarkably increased. Besides, the chelating capacity of the produced lipopeptide and metal ions is evaluated, the result shows that the binding content of zinc ions in the chelate reaches 478.2012 mg / 100g, and further bacteriostatic experiments show that the chelate can effectively inhibit growth of micrococcus luteus.

The invention discloses a strain of lactobacillus parabarium LN43 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus parabarium LN43 is GDMCC No.66615, the preservation unit is Guangdong Province Microbial Culture Collection Center, the preservation time is June 30, 2025, and the address of the preservation unit is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City. The lactobacillus parabarium LN43 has the advantages that the lactobacillus parabarium LN43 has the preservation number of GDMCC No.66615, the preservation number is GDMCC No.66615, the preservation unit is Guangdong Province, the preservation time is June 30, 2025, and the preservation unit is the June 30, 2025. The lactobacillus parabarium LN43 has relatively high acid resistance and a certain cholate tolerance. The lactobacillus parabarium LN43 has the advantages of high acid resistance and certain cholate tolerance. The strain has a very strong inhibition effect on pathogenic microorganisms such as staphylococcus aureus, salmonella pullorum, salmonella enteritidis, escherichia coli, pseudomonas aeruginosa, salmonella typhimurium, micrococcus luteus, acinetobacter baumannii and klebsiella pneumoniae, and is a broad-spectrum antibacterial microorganism.

The present invention provides a Bacillus velezensis SFT4 with antibacterial function and its application, belonging to the technical field of microbial application. Bacillus velezensis SFT4 has good inhibitory effects on Irpex lacteus, Aspergillus pallidus, Clostridium perfringens, Staphylococcus aureus, Micrococcus luteus, etc., and has a certain inhibitory effect on Salmonella. A probiotic preparation containing Bacillus velezensis SFT4 and its metabolites is provided, and a method for using the probiotic preparation is provided. The probiotic preparation is composed of Bacillus velezensis SFT4, Lactobacillus reuteri, Lactobacillus casei, and Lactobacillus plantarum. The application of the probiotic preparation in the livestock and poultry breeding industry can effectively prevent and control Clostridium perfringens and Escherichia coli, and the probiotic preparation can be made into a feed additive or directly fed through a drinking water line, and it is not easy to block the drinking and feeding pipeline.

The present invention relates to topical compositions comprising Micrococcus luteus Q24 and a viscosity modifier, the compositions having a viscosity of from about 2,500,000 to about 8,000,000 cP at 25 DEG C, and the use of these compositions for improving the appearance of skin or at least one sign of aging or reducing skin dryness.

The present invention belongs to the technical field of genetic engineering. Provided are an antimicrobial protein, and a coding gene and the use thereof. An amino acid sequence of the antimicrobial protein is a sequence as set forth in any one of SEQ ID NOs. 1-12, or a sequence with the same function as the sequence as set forth in any one of SEQ ID NOs. 1-12 after the substitution and / or deletion and / or addition of 1-30 amino acids, or a sequence with the same function as the sequence as set forth in any one of SEQ ID NOs. 1-12 after the addition of 1-20 amino acids to the C-terminus and / or N-terminus thereof. The produced antimicrobial protein provided can hydrolyze bacterial cell walls, enabling lysis of Micrococcus luteus. Upon verification, the antimicrobial protein can significantly inhibit or kill Gram-positive and Gram-negative bacteria and yeast, thereby providing a new antimicrobial protein preparation and a preparation method therefor for industries such as daily chemicals, food, and feeds.