Resuscitation medium capable of promoting growth of VBNC (viable but non-culture) bacteria and improving separation abundance, and preparation method and application thereof

Abstract

The invention relates to the field of microorganism screening and culturing, in particular to a resuscitation medium capable of promoting growth of VBNC (viable but non-culture) bacteria and improving separation abundance, and a preparation method and application thereof. The resuscitation medium capable of promoting growth of VBNC bacteria and improving separation abundance is composed of medium base fluid, bacterium fluid and soil fluid, the bacterium fluid is prepared by culturing micrococcus luteus on culturing fluid medium to the later period of log phase, contains resuscitation promoting factors Rpf after culturing fluid medium is removed by centrifuging to extract protein and is 0.5-30% of the medium base fluid according to the volume ratio, and the soil fluid is prepared by adding water to the soil which is dried, screened and subjected to impurity removal, sterilizing multiple times and filtering to obtain filtrate or centrifuging to obtain supernatant, and is 0.1-20% of the medium base fluid according to the volume ratio. By means of the resuscitation promoting factors Rpf and by adding the soil fluid to prepare the resuscitation medium, resuscitation of the VBNC bacteria can be promoted further, and abundance of separating the VBNC bacteria from the ecological environment is improved. The resuscitation medium and the preparation method thereof have the advantages of simplicity in operation and fast resuscitation.

Description

technical field [0001] The invention relates to the field of microbial screening and cultivation, in particular to a recovery medium for viable non-culturable (VBNC) strains and a preparation method and application thereof. Background technique [0002] VBNC (viable but non-culture) refers to the fact that some microorganisms can be detected in their habitat, but cannot be cultured artificially in the laboratory. In 1982, Xu Huaishu et al. first discovered and proposed the "viable but non-culturable state" (VBNC) of bacteria through the study of the survival rules of V.cholerae and E.coli, that is, the bacteria are under adverse environmental conditions. , its cells are usually shrunk into a spherical shape (recently many studies have found that some bacteria show increased volume and cell elongation), and cannot be grown and reproduced by conventional methods, but they still have metabolic activity and act as DNA synthesis inhibitors Under certain conditions, when cultured...

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Problems solved by technology

However, this document does not disclose what kind of recovery medium to use, and in practical applications, the effect of simple recovery-promoting factors on promoting the recovery and growth of non-culturable (VBNC) state bacteria and the isolation abundance has not been very significant.

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