158 results about "Metabolic activity" patented technology

The invention relates to the technical field of material testing, and particularly discloses an intelligent quality detection method and system for a micro-mineral bio-organic fertilizer, local thermal excitation is applied to a fertilizer sample through a micro-area thermal pulse excitation device, a gas release kinetic curve and a spectrum change track are synchronously collected, and a gas spectrum coupling data cube is constructed; performing differential transformation and modal decomposition on the time sequence feature set, extracting transient response feature vectors and generating an activity response distribution diagram; establishing component-activity correlation analysis, and decoupling biological metabolic activity and matrix background interference through feature separation and a comparative learning strategy to obtain a dynamic metabolic fingerprint; constructing a multi-dimensional feature space by using the dynamic metabolic fingerprints and the apparent characteristic parameters, and performing information aggregation and feature reconstruction by using a graph convolutional network to generate a comprehensive quality index; and finally, realizing quality grade judgment based on a quality characteristic pyramid structure, and establishing a dynamic early warning mechanism by analyzing time sequence evolution characteristics of the activity response distribution diagram.

The invention discloses a bone defect repair material suitability prediction method based on deep learning, and the method comprises the steps: carrying out the overlapping and uniform partitioning of a bone defect region and a host bone region in three-dimensional CT image data, carrying out the position coding and feature extraction of a plurality of sub-blocks through a three-dimensional visual Transform model of multi-scale position coding, and obtaining the anatomical and functional features; calculating a bone metabolism activity score and a systemic inflammation score based on blood detection data, and then constructing pathophysiological features; performing multi-round interaction on the anatomical and functional features, the pathophysiological features, the parameter data and the multidirectional multi-head cross attention layer, in each round, selecting one modal feature as a query vector, and selecting any modal feature in the rest modal features as a key vector and a value vector; and fusing the interaction features of each round, and determining the suitability score and the risk level of the bone repair material by combining the attention weight and the bone metabolism activity score in the multidirectional multi-head cross attention layer.

The invention discloses a medical image recognition method and system based on artificial intelligence, and the method comprises the steps: obtaining CT image data, pathological section data and PET metabolic activity data, introducing a GNN graph neural network to model the semantic association between multi-modal features, and outputting a fusion feature vector; inputting the fusion feature vector into a DSRN double-flow space-time recursive network, extracting 3D lesion morphological features of a single image by using a spatial flow, analyzing lesion growth kinetic parameters of a historical image sequence through a time flow, and generating a lesion malignancy probability index based on a gating fusion unit; based on the focus malignancy probability index, Monte Carlo Dropout sampling is utilized to generate a confidence interval, a clinically interpretable credibility score is output, and when the confidence is smaller than a threshold value, a low-confidence area is displayed for a doctor to check. The deep fusion of multi-source information is realized, the one-sidedness of a single mode is avoided, and the recognition accuracy and efficiency are improved.

The invention discloses a preparation method and application of athlete-derived bifidobacterium probiotics for intestinal barrier repair, and relates to the technical field of biochemistry and microorganisms, and the preparation method comprises the steps of special bacteria source sampling, directional separation and purification, molecular biological identification, antibiotic sensitivity evaluation and injury repair verification. According to the preparation method and application of the sportsman-derived bifidobacterium probiotics for repairing the intestinal barrier, the sportsman intestinal flora is used as an exclusive bacterial source, and the unique metabolic elite attribute and stress adaptability of the sportsman intestinal flora are utilized to directionally screen and obtain a bifidobacterium strain adaptive to an extreme physiological stress environment; the bottleneck that conventional probiotics are only suitable for a common physiological environment and the survival rate and activity in a stress state are greatly reduced is broken through, high survival rate and metabolic activity can be kept in extreme environments such as exercise heat stress and the like, and accurate repair of intestinal barriers is realized through multiple mechanisms; meanwhile, an exclusive screening, identification and safety evaluation system is established, so that the safety and functional specificity of the strain are ensured.

The invention provides a trapped person position state analysis method and system, and relates to the field of data processing. According to the method, building structure distribution, conductive fluid flow channels and metal component distribution in a disaster target area are constructed to form a background entropy flow ground state, and noise intensity probability distribution, a channel joint fluctuation relation and a time stability index are counted to generate a background entropy flow field. In the search and rescue stage, a background entropy flow field is converted into a controlled mode through active disturbance, multi-physical channel noise responses before and after disturbance are collected through a multi-field passive sensor network, and a multi-dimensional local entropy response vector is constructed. And combining the consistency, directivity and duration of the multi-noise channel to identify an initial suspected region and extract an entropy anomaly core region, finally inferring metabolic activity and posture limitation conditions according to a local entropy response cooperative relationship in different disturbance modes, and outputting a position state result of the trapped person. By implementing the technical scheme provided by the invention, the position state of the trapped person can be accurately identified.

The invention relates to the technical field of organelle separation, and provides a method for rapid purification of peroxisome and oxidation regulation analysis of ultra-long chain fatty acid. The method comprises the following steps: introducing a nucleic acid sequence for coding a fusion protein into a target cell to obtain a cell material, the fusion protein comprising a truncated sequence derived from a peroxisome membrane protein and a tag sequence for affinity purification; taking the cell material as a sample, and separating the peroxisome with the fusion protein from the cell by utilizing an affinity ligand on the basis of a high affinity binding principle. Therefore, according to the method disclosed by the invention, the peroxisome can be rapidly and efficiently enriched, and the whole purification process can be completed within 30 minutes, so that the experimental period is greatly shortened; core protein components of target organelles can be efficiently reserved, and the metabolic activity of peroxisome can be maintained through a complete membrane structure and an internal environment; meanwhile, the same purified sample can synchronously support proteome and metabolome analysis, and direct association of'protein-metabolism 'data is realized.

The invention discloses a method and a system for analyzing immune cell change of an HER2 variant lung cancer patient. The method comprises the following steps: collecting a peripheral blood sample and carrying out single-cell RNA (Ribonucleic Acid) sequencing; performing quality control, standardization processing, dimensionality reduction and clustering analysis on the sequencing data; carrying out immune cell subset annotation on the clustering result based on a preset immune cell marker; analyzing cell communication modes among different immune cell subpopulations by using a ligand-receptor database; performing quantitative statistics on the proportion of various immune cells based on sample grouping; and further performing subpopulation fine analysis, quasi-time sequence trajectory reconstruction and metabolic pathway activity evaluation on the CD8 + T cells to construct a comprehensive immune cell map. According to the method, the multi-dimensional characteristics of the peripheral blood immune cells can be presented at high resolution, and the comprehensive analysis of the functional state, the communication network and the metabolic activity of the immune cells is realized. According to the system and the device, automatic operation and visual display of the method can be realized. According to the invention, the immune monitoring efficiency can be improved, and an effective tool is provided for immune treatment effect evaluation and disease mechanism research.

The present application relates to the technical field of excrement treatment, and particularly relates to a feces treatment method based on flora analysis, comprising obtaining reaction temperature and material Ph value and determining gas production relative deviation based on gas production, determining device operation state based on characteristic parameters; in response to the device operation state, determining abnormal characteristic identification to obtain key functional flora parameters, determining flora balance index based on the key functional flora parameters to determine whether acid-producing bacteria and methanogenic bacteria are in flora balance; determining smoothness index according to the change curve of a plurality of actual flora balance indexes obtained in an initial detection period to determine the deviation degree of the flora balance index, and adjusting the correction sensitivity coefficient of the feed adjustment amount based on the smoothness index. The present application performs real-time monitoring and dynamic regulation on the excrement treatment process based on the flora analysis result to maintain optimal flora structure and metabolic activity, and improves treatment efficiency and stability.

Systems and methods for sensing vibrational absorption induced photothermal effect via a visible light source. A Mid-infrared photothermal probe (MI-PTP, or MIP) approach achieves 10 mM detection sensitivity and sub-micron lateral spatial resolution. Such performance exceeds the diffraction limit of infrared microscopy and allows label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells can be visualized. MIP imaging technology may enable applications from monitoring metabolic activities to high-resolution mapping of drug molecules in living systems, which are beyond the reach of current infrared microscopy.

This invention relates to the field of medical data mining technology, specifically to a system for assessing eye muscle fatigue in myasthenia gravis based on infrared thermal imaging. First, the degree of blood circulation impairment is determined based on the temperature changes of pixels in various directions within a speckled region. Then, based on the number of speckled regions and the concentration of hot spots, a speckled region influence value reflecting local blood circulation and metabolic abnormalities is determined. Next, combining the degree of blood circulation impairment, the speckled region influence value, and the speckled region distribution, a comprehensive reference degree of eye muscle fatigue is determined. Furthermore, considering the distribution of speckled regions under the influence of grease, the degree of blood circulation impairment, and the characteristic that the reference degree of eye muscle fatigue typically does not change, a corresponding grease influence value is determined. Thus, combining the reference degree of eye muscle fatigue and the grease influence value allows for a more accurate determination of the eye muscle fatigue assessment value, resulting in better assessment of eye muscle fatigue in myasthenia gravis based on this value.

This invention relates to the field of soil remediation technology, specifically disclosing a method for remediating heavy metal-contaminated soil using microbial agents. The invention uses hydroxyapatite as a carrier, utilizing its adsorption properties to load licorice extract onto its surface. To further improve the stability of the licorice extract, a carboxymethyl cellulose (CMC) solution is added. After stirring, mixing, and drying, the excellent film-forming properties of CMC help improve the stability of the licorice extract, while also increasing the specific surface area and adsorption sites of the hydroxyapatite. The introduction of licorice extract and CMC not only enhances the chelating ability of hydroxyapatite for heavy metals but also provides an additional carbon source for microorganisms, promoting their growth and reproduction. The metabolic activity of *Trichoderma solani* and *Bacillus licheniformis* is significantly enhanced, resulting in a substantial increase in remediation efficiency.

This application relates to the field of dairy food preparation technology, specifically to a room-temperature fermented milk and its preparation method. This application utilizes ultrasonic treatment on a composite system of milk base material and fermentation strains, and develops ultrasonic process parameters adapted to this composite system. This allows for the regulation of milk protein structure and the metabolic activity of fermentation strains, specifically inhibiting the generation of nitrogen oxides to reduce potential health risks. The ultrasonic treatment process of this application is short, easily controllable, and easily integrated with existing fermented milk production lines, possessing good potential for large-scale production. Furthermore, by ultrasonically treating the composite system of milk base material and fermentation strains under specific parameters, this application can also optimize the protein gel network structure to improve texture, while simultaneously increasing the content of sulfides, alcohols, aldehydes, ketones, and aromatic compounds, thus optimizing product flavor.

Disclosed in the present invention is use of an organometallic macrocyclic compound molecule in the catalytic enhancement of the level of intracellular oxidized coenzyme I and in an anti-aging product. The organometallic macrocyclic compound molecule of the present invention enters a cell and catalyzes the conversion of reduced coenzyme I within the cell into oxidized coenzyme I, thereby enhancing the metabolic activity of the cell and prolonging the lifespan of aging animals. The organometallic macrocyclic compound molecule is applied to oxidized coenzyme I supplements, anti-aging drugs, and health care products. By means of daily oral administration, the organometallic macrocyclic compound molecule can improve the metabolic activity of the aging population, thereby maintaining their physiological health and prolonging their lifespan.

The invention discloses a rumen fibroblast line as well as a construction method, a culture method and application thereof, the rumen fibroblast line G8 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: C2025332, the rumen fibroblast line G8 has a typical fibroblast-like form and can be stably passaged, and the rumen fibroblast line G8 can be used as a rumen fibroblast cell line. The cell culture medium also has the characteristic of highly expressing fibroblast marker genes and the function of tolerating and utilizing rumen core metabolite butyric acid, and the proliferation rate and metabolic activity of the cell culture medium under the condition that 15mmol / L butyric acid is used as a main energy source are obviously superior to those of glucose culture; according to the construction method of the rumen fibroblast line, the steps of function screening and molecular identification are introduced, so that the identity authenticity of the constructed cell line can be ensured; the culture method of the rumen fibroblast line is simple and effective, culture medium materials are easy to obtain, and culture conditions are easy to realize; the rumen fibroblast line can be used for rumen in-vitro research, and is beneficial to clarification of a mechanism for regulating rumen interstitial metabolism and tissue repair by butyric acid.

This invention discloses a method and system for controlling the preparation of low-temperature, high-efficiency straw-promoting microbial agents, relating to the field of microbial agent preparation process control technology. It utilizes multiple types of sensors to collect a microbial agent preparation dataset RAW(t) at time t, calculates and constructs a metabolic feature vector Vm(t) at time t, achieving real-time quantification of the macroscopic metabolic activity of microorganisms. Furthermore, it calculates the respiratory entropy Q at time t. R (t) and calculate the enzyme synthesis potential E at time t. S (t) and construct the diagnostic state vector Ds(t) at time t, thereby transforming external measurable parameters into a deep insight into the internal production potential of the microbial cells, objectively and dynamically diagnosing the fermentation process into different physiological stages, and generating a stage identifier ID(t) at time t, automatically executing the matching fine control strategy AIR, ensuring that optimal regulation is automatically adopted at each stage, thereby significantly improving the quality, production efficiency and batch stability of the microbial agent product.

This invention discloses a method for assessing the toxicity of antibiotics to green algae based on Fourier transform infrared spectroscopy, comprising: constructing a multidimensional experimental dataset containing different antibiotic concentrations and exposure times under controlled conditions, and simultaneously acquiring raw infrared spectra and degradation efficiency data of green algae. The spectral data are preprocessed to extract toxic response features characterizing cell damage and metabolic feedback features reflecting metabolic activity, forming a dual-path spectral feature set. A heterogeneous information network containing toxic effect edges and degradation feedback edges is constructed using antibiotic concentration and green algae state as nodes. A toxicity joint inference model is constructed based on a graph neural network, and the model is trained using the heterogeneous information network to achieve synergistic analysis of toxic effects and degradation potential. The trained model is used to analyze the target water area situation, simulate the purification process, and generate a dynamic river purification planning report including nodes for biomass maintenance and auxiliary intervention measures, providing comprehensive and accurate purification decision support.

The invention relates to a system for determining cellular respiration and nutrient metabolism of living cells or tissues, the system comprising: a cell container configured to receive and maintain a biological sample; a gas volume in fluid communication with the cell container, the gas volume containing gaseous components produced by metabolic activity of the biological sample; an optical detection unit configured to detect a target gas species in the gas volume by wavelength-selective absorption spectroscopy, the optical detection unit being further configured to distinguish between different isotopic species of the target gas species, the optical detection unit comprising an illumination source and detector arrangement optically coupled to the optical detection unit and configured for measurement of absorption signals corresponding to different isotopes of the target gas species; and an electronic control and evaluation unit operatively connected to the optical detection unit, the electronic control and evaluation unit being configured to acquire and process absorption data, determine absolute values and / or temporal variations of the target gas species concentration and isotopic composition, and output respiration-related parameters of the biological sample. In this way, a system is provided that enables precise and efficient determination of carbon dioxide produced by living cells or tissues under physiological conditions.

This invention discloses a method for promoting the biodegradation of N,N-dimethylformamide and simultaneous denitrification. The method constructs a zero-valent iron (ZFE)-coupled biological treatment system, which not only accelerates electron transfer efficiency but also significantly increases the activity of DMF-degrading enzymes and nitrate reductases, synergistically promoting electron transfer and microbial metabolic activity, thereby enhancing the removal of DMF and nitrate nitrogen. Compared to biological treatment systems that only add activated sludge without adding ZFE, adding ZFE significantly improves the removal efficiency of DMF and nitrate nitrogen, making it suitable for the treatment of large-scale polluted water bodies or industrial wastewater.

The invention discloses a fermentation preparation method of gastrointestinal tract health care traditional Chinese medicine for animals in the technical field of animal feed additives. The method comprises the following steps: crushing traditional Chinese medicines such as astragalus membranaceus, poria cocos, bighead atractylodes rhizome, hawthorn, pericarpium citri reticulatae and liquorice, mixing the crushed traditional Chinese medicines with wheat bran, adding composite probiotics and unique dynamic covalent bonding plant polysaccharide polyphenol three-dimensional network carrier material fine powder, humidifying, fermenting at constant temperature, and finally drying and crushing to obtain the product. The key point is that the carrier material is formed by assembling oxidized sodium alginate, hydrazide carboxymethylated yeast glucan and tea polyphenol tannic acid composite nano-colloid through the synergistic effect of dynamic covalent bonds and hydrogen bonds. The material not only can remarkably improve the survival rate and metabolic activity of probiotics in a fermentation system and promote conversion of active ingredients of traditional Chinese medicines, but also has intelligent responsiveness and can realize targeted slow release in animal intestines, so that the gastrointestinal tract health-care function of a product is comprehensively enhanced, and the growth performance of animals is improved.

The application discloses a probiotic composition and a production process of a probiotic fermented bread crumb, and belongs to the technical field of bread crumb processing. The probiotic composition is a microcapsule structure containing malt dextrin, arabic gum and heat-denatured whey protein isolate as wall materials, and lactobacillus acidophilus and lactobacillus rhamnosus as core materials. The structure can ensure that a small amount of live bacteria and metabolic products are slowly released through the micropores of the wall materials in a normal temperature fermentation environment, so that the free lactic acid bacteria are prevented from rapidly proliferating, excessively producing acid and competing with yeast for carbon and nitrogen sources in the initial stage of fermentation. The probiotic composition can provide nutrition for the yeast in the fermentation process, promote cell division and metabolic activity of the yeast, and the polydextrose, inulin and arabic gum in the microcapsule wall materials are hydrophilic colloids, which can form a uniform hydration network in the dough.

The present invention relates to a method for reducing WST-8 to formazan based on NAD(P)H for determining the susceptibility of certain bacterial isolates to infection by certain bacteriophages, thereby allowing direct observation of the results measured as cell death of the target bacteria. The reduction of tetrazolium salts to formazan by NAD(P)H is used to determine the metabolic activity of cells and as an indicator of cell viability; whereas WST-8 is used to qualitatively measure the bacteriophage killing activity of the target bacteria. The present invention thus belongs to the field of medical biology, in particular to in vitro laboratory test methods.

The present disclosure provides methods for inducing cellular quiescence—defined as a reversible arrest of cell proliferation—by inhibiting ribosome biogenesis. The methods involve administering a quiescence-inducing agent, such as a small molecule inhibitor, peptide, antisense oligonucleotide, or targeted degrader, that interferes with rRNA transcription, processing, ribosomal protein synthesis, or ribosome assembly. The agent may be applied to cultured cells, tissues, organs, or organoids, across a range of cell types and species. In some embodiments, the methods reduce metabolic activity and enhance stress resistance in non-dividing or post-mitotic cells. By slowing proliferation and / or metabolism while preserving viability, these methods enable improved preservation of cells, tissues, and organs-enhancing transplantation logistics, biobanking, regenerative medicine workflows, and biological transport. Additional applications include studies of cell cycle regulation, tissue regeneration, stress biology, aging, and induction of organismal hypometabolism (e.g., torpor or hibernation). The disclosure includes examples demonstrating quiescence induction using chemical biology systems, pharmacological agents, and genetic approaches.

This invention discloses an intelligent nutrition recommendation method for cancer patients based on metabolic rhythm synchronization, belonging to the field of nutrition recommendation. This invention integrates temporal physiological data and clinical information, extracts features, and uses a bidirectional long short-term memory network to predict the host metabolic activity curve. Based on a tumor knowledge graph, a conditional generative adversarial network is constructed to infer and generate a tumor metabolic activity curve using the host curve, tumor type, and treatment stage as conditions. A temporal separation potential heatmap is generated by calculating the phase difference and overlap between the two curves. Using the heatmap and clinical labels as states, an integrated constraint proximal strategy optimization algorithm is used for decision-making, outputting a time-segmented nutrient delivery action sequence that satisfies temporal nutritional constraints. Using this sequence as the target, an optimization algorithm performs a multi-objective search on a recipe database to generate a personalized daily recipe that considers both nutritional and taste preferences, providing cancer patients with timely and intelligent decision-making.

The application discloses an evaluation system for an epilepsy electroacupuncture treatment target based on an amygdala nerve response prediction, relates to the technical field of nerve regulation and brain function modeling, and realizes the multi-modal synchronous collection of brain region potentials, blood oxygen and metabolic activities through high-density EEG, electrophysiological sensors, fMRI and PET, and constructs a standardized nerve response dataset and a brain region function mapping matrix. An amygdala prediction response index is calculated and compared with a response threshold, a stimulation response compliance judgment and parameter correction are realized. The nerve response propagation delay, cooperative coupling coefficient and epilepsy wave front tracing time are calculated, a comprehensive target evaluation coefficient is obtained, and effective target points are screened. Based on the treatment scheme implementation of the effective target point set, the nerve feedback data in the continuous treatment cycle is monitored in real time, and a nerve plasticity correction index is calculated, compared with a plasticity stability threshold, an adaptive feedback regulation closed loop is formed, and precise prediction and dynamic optimization control of epilepsy electroacupuncture targets are realized.

The application relates to the technical field of adaptive control, in particular to an intelligent control method and system for a proofing and steaming integrated cabinet, which comprises the following steps: taking the concentration of carbon dioxide in the proofing and steaming integrated cabinet as a fermentation degree, dynamically adjusting the membership function of each output fuzzy set in a fuzzy PID control model according to the fermentation degree, and obtaining an initial output; taking the concentration change rate of carbon dioxide as a fermentation activity index representing the metabolic activity of yeast, determining an integral inhibition coefficient according to the fermentation activity index, and inhibiting the integral gain adjustment amount in the initial output according to the integral inhibition coefficient to obtain a control output; and adjusting the parameters of a PID controller according to the control output to realize adaptive control of the proofing and steaming integrated cabinet. Through the technical scheme, accurate adaptive control of the proofing and steaming integrated cabinet can be realized in the food fermentation process.

The present application relates to the field of animal nutrition and microbiology, and in particular to a nutrition intervention method for regulating eggshell mineralization by egg chicken intestinal flora-metabolites, a preparation, a sustained-release granule and an application thereof, comprising the following steps: A. intestinal flora homeostasis establishment period: adding a bile acid precursor complex, a flora regulating complex and an intestinal environment regulator in the feed of 24-week-old laying hens at a proportion of 0.5%, continuously for 14 days; B. metabolite production enhancement period: adding a metabolite precursor complex and a metabolic activity regulator to the feed of laying hens at the peak of egg production at a proportion of 0.8%; C. shell gland mineralization efficiency improvement period: adding a calcium transporter activating complex, a carbonic anhydrase cofactor and an ion channel regulator to the feed of laying hens at the later stage of egg production at a proportion of 1.0%, to promote the production of key metabolites and activate the whole process molecular mechanism of shell gland mineralization.

The invention relates to the technical field of microbial detection, in particular to a method for evaluating fermented grain microbial activity, which comprises the following steps: 1, pretreating fermented grains to obtain microorganisms in the fermented grains; secondly, heavy water incubation is conducted on microorganisms in the fermented grains obtained in the first step, the heavy water incubation comprises the steps that culture is conducted for 1-5 h through a heavy water-containing culture medium, the heavy water content in the heavy water-containing culture medium is 60%-80%, a culture medium adopted by the heavy water culture medium is a fermented grain leach liquor culture medium, and the heavy water content in the fermented grain leach liquor culture medium is 20%-30%; the fermented grain leach liquor culture medium comprises a yeast extract and fermented grain leach liquor; and 3, carrying out interference removal treatment on the microorganisms obtained by heavy water incubation in the step 2, and carrying out activity detection on the microorganism suspension obtained after interference removal treatment. The invention establishes a set of research method for fermented grain microbial function and metabolism in-situ activity, and the metabolism activity of the flora can be monitored in real time under the condition that the natural state of the fermented grain flora is not destroyed.

The invention discloses low-temperature injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. Hydrogel is prepared from high-molecular-weight hyaluronic acid grafted with vitamin E and poly (N-isopropylacrylamide), the hydrogel is in an injectable liquid state at 4 DEG C, and a drug capsaicin (CPZ) wrapped by micelle is loaded and injected into the brain from the brain in a stereotactic mode. The pharmaceutical composition can temporarily maintain local low temperature of brain tissue and release capsaicin, reduce local metabolic activity of ischemic brain tissue, temporarily inhibit biogenesis, reduce NADH, FADH2 and other reducing equivalents and ADP accumulation, reduce the possibility of electron leakage in an electron transport chain, and improve the bioavailability of ischemic brain tissue. Vitamin E is added to reduce superoxide anions (O2 <.->) generated by reduction reaction of oxygen and leaked electrons in the reperfusion process, and vitamin E can promote ROS removal, synergistically alleviate oxidative stress and subsequent inflammation, and comprehensively improve cerebral ischemia reperfusion injury.

The present application relates to the technical field of microbial immobilization, and discloses a preparation process of a specific carrier immobilized multifunctional microbial flora composition, comprising: mixing multifunctional microbial flora with an inducer to produce extracellular polymers, to obtain pretreated bacterial solution; adding carboxymethyl deacetyl chitin to the pretreated bacterial solution and adjusting the pH value, to form a primary wrapping suspension; immersing a porous carrier in the primary wrapping suspension, spraying a sodium phytate and calcium lactate composite aqueous solution under a pressure difference environment, and constructing a concentration gradient crosslinking network on the surface and pores of the carrier through ion bridging, wherein the present application utilizes component protonation response mechanism and nonlinear crosslinking distribution to actively open diffusion channels under a specific acid-base environment, reduce metabolic product discharge resistance, and maintain long-term metabolic activity and structural stability of the microbial flora.

According to the rice field carbon emission reduction and yield increase synergistic system and method, EC11 bacteria inhibit methane bacteria by occupying ecological niche, and micromolecule water treated by fullerene not only optimizes the soil microenvironment (the volume weight is reduced by 12%), but also can promote the metabolic activity of the EC11 bacteria (the respiration rate is increased by 25%) to form 1 + 1gt; and 2, the synergistic effect is achieved. Meanwhile, a carbon monitoring and trading system is pre-embedded into a planting process, and a'planting and trading 'mode of paddy field carbon assets is realized for the first time.