31 results about "Bioleaching" patented technology

The invention discloses a method for removing heavy metals in sludge, and belongs to the field of environmental protection technology and solid waste recycling. The method comprises the following steps: adding a bioleaching bacteria solution and a nutritive salt solution into dewatered sludge, and adjusting the pH value to form mixed slurry; applying an asymmetric alternating-current electric field to the mixed slurry to perform biological-electrochemical coupling treatment, and performing solid-liquid separation on the treated slurry to respectively obtain purified sludge and leachate containing heavy metals. By utilizing polarization inhibition and oscillation mass transfer effects of the asymmetric alternating-current electric field, the removal efficiency of the bioleaching on the heavy metals in the sludge, especially the organic combined state and residual state heavy metals, is remarkably enhanced, and the method has the outstanding advantages of short treatment period, low energy consumption, no electrode polarization and high heavy metal removal rate.

The invention relates to the technical field of solid waste biological treatment, and discloses La and Ce toxicity resistant thiobacillus thiooxidans and application thereof, and the collection number of the thiobacillus thiooxidans is GDMCC No: 63522. The invention provides a novel thiobacillus thiooxidans, and the preservation number of the novel thiobacillus thiooxidans is GDMCC No: 63522. The strain can highly tolerate La and Ce, and can realize biological leaching of solid wastes rich in La or (and) Ce under high ore pulp density, so that more solid wastes can be treated in a single batch, the biological leaching rate is improved, and the strain has a good application prospect.

The present application relates to the technical field of health, environmental management and resource development, and discloses an alkaline-sulfur oxidizing bacteria and its application in water pollution treatment and bioleaching. The alkaline-sulfur oxidizing bacteria is named 381-2, which has been preserved in the China General Microbiological Culture Collection Center on December 23, 2024, and the preservation number is CGMCC No. 33017; the microbial classification name is Stutzerimonas sp. The present application isolates a new species of Stizolobium strain from a deep-sea hydrothermal area Stutzerimonas sp. . The strain has strong and rapid oxidation ability of thiosulfate ions, and also has the characteristics of producing alkali, so it can be applied to the fields of water pollution treatment and bioleaching.

A bioleaching reactor for removing heavy metals in industrial sludge relates to the technical field of environmental protection equipment, and is characterized in that a venturi mixer is fixedly arranged on the left side of the upper part of a reaction cylinder, and two liquid inlets of the venturi mixer are respectively connected with a sludge conveying pipe and a bacterial liquid conveying pipe; a material guide frame is fixedly arranged at the position, located above the solid-liquid separation assembly, in the reaction cylinder, the motor is fixedly arranged on the upper portion of the reaction cylinder, a stirring shaft is rotationally arranged on the top wall in the reaction cylinder through a bearing, a plurality of stirring blades are connected to a connecting disc through a self-adaptive adjusting mechanism, and spiral propelling pieces are fixedly arranged on a plurality of connecting rods. The stirring shaft is provided with a paddle at the position on the other side of the connecting rod, so that the three-dimensional efficient mixing of the sludge and the bacterial liquid can be realized, the dead angle of mixing is reduced, the angle of the stirring blade can be automatically adjusted through the centrifugal force or resistance during stirring, the shearing force on the high-viscosity sludge is enhanced, the good mixing effect between the sludge and the bacterial liquid can be achieved, and the stirring efficiency is improved. The reaction efficiency is improved.

The present invention relates to a method for recovering metals from waste batteries by using a magnetic field-applied bioleaching process. According to the present invention, by applying a magnetic field to a bioleaching process, it is possible to improve the recovery rate of metals, and to leach valuable metals. In addition, the method is environmentally friendly compared to a dry melting process or an acid leaching process, which has been mainly used for metal recovery, and is practical due to a simple process thereof. Therefore, the method for recovering metals of the present invention may recover metals from wastes in waste resources field such as waste batteries and waste catalysts and turn the metals into resources.

The application discloses a method for bioleaching copper and zinc in lead-zinc smelting slag, which comprises adding the lead-zinc smelting slag into an acid culture medium inoculated with acidophilic leaching bacteria domesticated under metal salt stress for a certain period of time. Compared with the prior art, the acidophilic leaching bacteria domesticated under high-concentration metal salt stress can realize efficient leaching of copper and zinc in low-grade lead-zinc smelting slag, realizes waste-to-resource, and converts the original environmental burden into valuable resources, which conforms to the concept of sustainable development and circular economy, and has the advantages of environmental friendliness and low operation cost.

The invention relates to a microorganism-based ionic rare earth ore extraction method in the technical field of rare earth extraction, and the method comprises the following steps: step 1, culturing a microorganism Burkholderia contaminans GP4-1 to obtain an ore leaching bacterial liquid containing microorganisms and metabolites thereof; secondly, the ore leaching bacterial liquid prepared in the first step makes contact with ionic rare earth ore, and a biological leaching reaction is carried out; and thirdly, a leaching mother solution rich in rare earth elements is collected. By means of the method, selective enrichment of heavy rare earth can be achieved.

This application provides a bioleaching method for low-grade copper ore, comprising the following steps: crushing copper-bearing ore and pyrite separately, screening to obtain copper ore powder and iron ore powder, mixing the copper ore powder and iron ore powder and piling them in a heap leaching column, and burying vertical pipes in the heap to obtain a heap; spraying the heap with acid, then inoculating with leaching bacteria solution to carry out the heap leaching process; the leaching process uses raffinate for circulating leaching. The method of this application uses bioleaching to treat low-grade copper ore, which has the advantages of high leaching rate, low pollution, water conservation, and ease of operation. It overcomes the disadvantages of traditional flotation methods for separating low-grade copper ore, which are not only difficult to process but also have extremely low flotation yields, making it difficult to obtain high-grade copper concentrate.

The present application relates to a kind of vacuum roasting coupling microbial upgrading no-bonding high-sulfur bituminous coal preparation silicon smelting reducing agent method, belong to industrial silicon smelting composite reducing agent technical field.The present application mixes even with bituminous coal powder and corn cob powder and obtains mixture A, hydrogen peroxide solution is added in mixture A and grinds and mixes evenly and obtains mixture B;Mixture B is placed in temperature 800~1200 ℃ under vacuum roasting 30~90min, with furnace cooling and obtains roasting mixture C;Deionized water, bacteria liquid and sodium silicate are added to roasting mixture C, and carry out microbial leaching at temperature 25~65 ℃ for 5~10h, and stratify after standing, remove supernatant;Repeat microbial leaching step until supernatant is neutral, solid-liquid separation, and solid drying obtains low-sulfur composite reducing agent.The low-sulfur composite reducing agent obtained by the present application reaches 91.2~93.7%, and the caking index is 28~53, and the reaction activation energy is 13.4~17.7KJ / mol, meet the requirements of industrial silicon composite reducing agent.

This invention discloses a method for improving the bioleaching efficiency of heavy metal contaminated soil using a carbon rod electrode system modified with graphene oxide (GO). The invention involves preparing a carbon nanomaterial, GN-GO, from traditional graphene rod electrodes using a GO suspension through a multiple soaking and drying process. The GN-GO material is then used as the anode, and the unmodified graphene rod electrode as the cathode. The anode and cathode, connected to an external power source via wires, are placed in a container containing a culture medium and a heavy metal contaminated soil sample. An acclimatized bacterial strain is then introduced, and leaching is performed at 25–30°C and 120–150 r / min. This invention improves the conductivity and specific surface area of ​​the electrode by altering the electrode surface structure of the bioelectrochemical system, while simultaneously optimizing the biocompatibility of the electrode material. The method combines electrochemical and biological approaches, resulting in high remediation efficiency and good remediation effects for heavy metal contaminated soil.

This invention relates to the field of environmental engineering technology and discloses a method for treating wastewater containing copper nitrate and copper sulfate from the high-purity copper industry. The method involves filtering the copper nitrate and copper sulfate wastewater into reaction tanks A and B respectively, adding flocculants, monitoring the pH value of the reaction tanks during the reaction process using online monitoring instruments, and separately adding hydrogen peroxide to reaction tank A to break down the complexed copper and convert it into Cu. 2+ The supernatant is used as effluent and treated by diffusion dialysis. Activated carbon adsorption and reverse osmosis separation are used to further remove residual copper ions. The sludge is washed eight times with circulating water. Based on sludge stabilization, the sludge is converted into organic fertilizer through composting. Bioleaching technology uses microorganisms or their metabolites to leach valuable metals from the sludge through interaction with the metals. The treated wastewater is neutralized and then discharged into a comprehensive wastewater pond. The recovered copper is electrolyzed to produce copper products with 98% purity.

This invention relates to a method for separating and recovering arsenic and iron from arsenic- and iron-containing bioleaches. The specific steps are as follows: An adsorbent is added to the arsenic- and iron-containing bioleach, mixed thoroughly, and reacted. After adsorption is complete, the solid and liquid phases are separated to obtain an arsenic-iron solution free of bacterial residues. A reducing agent is added to the obtained arsenic-iron solution. After detection, all arsenic and iron are converted to trivalent and divalent valences, respectively, and the reaction is complete, yielding a solution containing low-valent arsenic and low-valent iron. An extractant is added to the reduced arsenic-iron solution to obtain an arsenic-containing organic phase and an iron-containing raffinate. The iron-containing raffinate is returned to the bioleaching process, and the organic phase is back-extracted to obtain an arsenic-containing back-extract. The back-extracted organic phase is reused to extract arsenic. Sodium hydroxide is added to the arsenic-containing back-extract, mixed thoroughly, and then benzyl chloride is added. After stirring and reacting, the mixture is allowed to stand for layering and the acidity is adjusted for crystallization. The crystals are washed and dried to obtain benzylarsine product. This invention solves the shortcomings of existing neutralization processes, such as large slag volume, difficulty in product purification, complex processes, and low arsenic and iron recovery rates. It fully utilizes the arsenic and iron resources in the bioleaching solution and has the advantages of high recovery rate, high product added value, no secondary pollution generated in the process, and low investment.

This invention provides a method for using enzymes to cyanide a metal and reduce the metal-cyanide complex, as well as a method for using biological techniques to hydrolyze cyanide. [Solution] The present invention relates to the use of genetically modified bacteria in a process comprising the generation of cyanide, the reduction of leached metal ions, the decomposition of cyanide, and the subsequent reuse of cyanide. Furthermore, it provides a transcriptional regulatory tool in Chromobacterium violaseum. In one embodiment, the present invention provides a genetically modified bacterium that is isolated and transformed by at least one polynucleotide molecule, wherein the polynucleotide molecule comprises a heterologous mercury(II) reductase (MerA) gene operably linked to at least one promoter, and comprises at least one mutation in which a metal ion is reduced by the gene product of the MerA gene to produce a metal element as metal nanoparticles.

This invention provides a method for recovering metallic copper by atmospheric pressure oxygen-enriched leaching, relating to the field of hydrometallurgy. The method includes: mixing copper sulfide concentrate with a sulfuric acid solution to obtain a mixed slurry; introducing an oxygen-rich gas under atmospheric pressure to carry out a leaching reaction; and after the reaction, separating the liquid and solid to obtain a copper-containing leachate. By using the synergistic leaching of sulfuric acid solution and oxygen-rich gas under atmospheric pressure, this method eliminates the reliance on expensive high-pressure equipment, removes the safety hazards of high-pressure explosions, and significantly reduces equipment corrosion and investment costs. Using oxygen as a highly efficient oxidant, this method significantly shortens the extraction cycle, overcomes the drawbacks of time-consuming bioleaching and the generation of waste, and achieves efficient, safe, and low-cost extraction of metallic copper from copper sulfide concentrate.

The invention relates to a method for separating and recycling rare earth and a leaching agent in rare earth-containing bioleaching liquid, which is characterized by comprising the following steps: 1) obtaining the bioleaching liquid rich in rare earth; 2) removing impurities; 3) activating a coordination chemical bond; 4) activating the transformation material; (5) fracture and reconstruction of coordinate bonds; and 6) cyclic utilization. Based on the principles of activation, fracture, reconstruction and the like of a bioleaching agent-rare earth coordination chemical bond, rare earth elements in the bioleaching agent can be efficiently separated and recovered (the rare earth recovery rate is close to 100%) in a transformation separation mode; in the rare earth separation and recovery link, a bioleaching agent-rare earth coordination chemical bond is broken, transformation of a rare earth bioleaching agent and selective separation of rare earth elements are achieved, the bioleaching agent is reserved in a solution and can be used for subsequent leaching of rare earth raw materials, and recycling of the bioleaching agent is achieved (the regeneration rate is close to 100%); chemical reagents do not need to be added, so that introduction of environmental pollution substances is avoided; the method is simple in equipment and operation, good in effect, low in cost, environment-friendly and convenient for large-scale industrial application.

The invention discloses a method for recycling copper and zinc from high-iron low-grade copper-zinc ore through biological leaching and application, and relates to the technical field of comprehensive utilization of mineral resources. The method for recycling copper and zinc through biological leaching of the high-iron low-grade copper-zinc ore comprises the steps that a mixed flora containing ferrous oxide thiobacillus, thiobacillus thiooxidans, leptospirillum ferriphilum and acidithiobacillus caldus is domesticated, and ore leaching bacterial liquid is obtained; the method comprises the following steps: carrying out biological leaching treatment on a mixture of high-iron low-grade copper-zinc ores by adopting an ore leaching bacterial solution, reducing Fe < 3 + > in a leaching solution into Fe < 2 + >, removing iron in a reducing solution by combining a potential-controlled goethite method, and finally, sequentially recovering copper and zinc. The method can realize efficient recovery of copper and zinc, and has the advantages of environmental protection and low cost.

The invention provides a low-grade copper ore bioleaching method which comprises the following steps: respectively crushing and sieving copper-containing raw ore and pyrite to obtain copper ore powder and iron ore powder, mixing the copper ore powder and the iron ore powder, stacking in a dump leaching column, and burying a vertical pipe in an ore heap to obtain the ore heap; spraying the ore heap with an acid solution, then inoculating the ore leaching bacteria solution, and carrying out a heap leaching process; raffinate is adopted for cyclic leaching operation in the leaching heap process. According to the method, the biological leaching method is adopted for treating the low-grade copper ore, the method has the advantages of being high in leaching rate, small in pollution, capable of saving water resources and easy to operate, and the defects that when the low-grade copper ore is separated through a traditional flotation method, the treatment difficulty is large, the flotation income is extremely low, and high-grade copper concentrate is difficult to obtain are overcome.

The invention belongs to the technical field of resource utilization, and discloses a resourceful treatment method of manganese slag, which comprises the following steps: carrying out oxidation pretreatment on the manganese slag to obtain solid-phase residue and filtrate I; the solid-phase residues are subjected to reduction leaching, and reduction leaching residues and filtrate II are obtained; the reduction leaching residues are subjected to weak acid elution, and elution residues and filtrate III are obtained; the elution residues are subjected to biological leaching, and biological leaching residues and filtrate IV are obtained; and combining the lixivium in each stage, and purifying to obtain a manganese-rich solution. According to the method, manganese in different occurrence forms is treated in a step-by-step and targeted manner through a multi-stage sequential elution process of manganese slag oxidation pretreatment, reduction leaching and chemical-biological combined fine washing, and the total recovery rate of manganese is remarkably increased.

The present application belongs to the field of hydrometallurgy, and relates to a method for modifying biomass to strengthen bioleaching of ion-adsorbed rare earth ore, which is carried out according to the following steps: modifying biomass with a sulfuric acid solution with a mass concentration of 40% to 80% to obtain a modified biomass solution; mixing the modified biomass solution with Czapek's liquid medium to obtain a mixed culture solution; inoculating the mixed culture solution with mixed microbial strains to obtain a fermentation solution; mixing the fermentation solution with ion-adsorbed rare earth ore, stirring, filtering, and obtaining the supernatant as a rare earth leaching solution. The present application uses the modified biomass solution to provide a nutrient medium and a fermentation substrate for microorganisms, improves the activity of the microorganisms and the yield of organic acids, and has the characteristics of low cost, wide raw material sources, and small environmental pollution.

The invention is a method of metal extraction using reductive electro-bioleaching of oxide minerals. This method of mineral treatment allows non-acidophilic soil bacterial consortia consisting of metal-reducing microorganisms growing at neutral pH to reduce the metal oxide mineral selectively, in the presence of controlled redox potentials at which the organism efficiently reduces the oxide minerals. The described reactor arrangement simultaneously allows precise anaerobic conditions and optimal agitation, enabling microbially assisted efficient electroreduction of oxide minerals. The invention is a scalable and cost-effective method of extraction of critical metals from their oxide forms and minerals.

The application provides a method for extracting manganese and silver from oxidized manganese silver ore and acidophilic Thiobacillus, and the method comprises the following steps: step 1, crushing and finely grinding the oxidized manganese silver ore and reducing minerals, and then making a slurry to obtain a mineral slurry; step 2, adding sulfuric acid into the mineral slurry and performing chemical leaching, and then performing solid-liquid separation on the obtained chemical leaching product to obtain a chemical leaching residue and a manganese-rich low-acid chemical leaching liquid; step 3, inoculating the chemical leaching residue with the acidophilic Thiobacillus and then performing biological leaching, and then performing solid-liquid separation on the obtained biological leaching product to obtain a low-manganese high-acid biological leaching liquid and a silver-containing biological leaching residue; and step 4, leaching silver from the silver-containing biological leaching residue. The method provided by the application is a full-wet extraction process, and is particularly suitable for treating low-grade oxidized manganese silver ore, can help to reduce the mining boundary grade of manganese silver resources, can improve the resource utilization efficiency, and can obtain greater economic benefits, social benefits and environmental benefits.

The invention discloses a method and system for leaching metal in a waste lithium battery positive electrode material through microorganism-electrochemical coupling, and belongs to the technical field of wet leaching. And the leaching efficiency of the metal in the waste lithium battery positive electrode material is remarkably improved. Compared with a traditional pure biological leaching technology, the leaching rate of lithium can reach 99% or above, the leaching rate of cobalt, nickel and manganese can reach 98% or above, and compared with the traditional biological leaching technology, the metal recovery rate is increased by 16%-22%. Meanwhile, the leaching reaction period is greatly shortened to be within 24-48 h, the problem that the traditional biological leaching reaction rate is low is effectively solved, and the turnover efficiency of industrial production is improved.

The application discloses a method for green extraction and recovery of platinum group metals from solid waste and low-grade ores, which is characterized by coupling of direct bioleaching enrichment of sulfides and indirect bioleaching enrichment of oxides, pre-treatment of ball milling, gravity separation and flotation, and post-treatment of impurity removal, adsorption and electrolysis. The method realizes green extraction and recovery of low-content platinum group metals in metal-based solid waste and low-grade ores through a series of processes such as gravity separation, flotation, bioleaching-circulating enrichment, adsorption and electrolysis. In the MBR regeneration tank, the direct leaching enrichment of platinum group metals in sulfides is realized by replacing sulfide materials, the indirect leaching enrichment of platinum group metals in oxides is realized by indirect leaching of oxide materials and regeneration of failed leaching liquid, and the direct leaching enrichment of sulfides and the indirect leaching enrichment of oxides are coupled to realize the synergistic leaching and efficient liquid enrichment of platinum group metals in low-content solid waste and low-grade ores.

The invention discloses a method for leaching different types of waste lithium ion battery positive electrode powder by using oral flora, and belongs to the technical field of resource recovery. The method comprises the following steps: firstly, preparing waste battery positive electrode powder and preparing a culture medium, carrying out gradient domestication and reactivation on the obtained oral flora, inoculating the activated flora into a leaching culture medium, constructing a biological leaching reaction system for culturing, filtering a sample, collecting filtrate and detecting the concentration of metal ions in the filtrate. Compared with a traditional biological metallurgy process depending on single eosinophilic property, the method depends on a metabolism cooperation mechanism among multiple species in the flora, and a mild weak acid leaching environment can be constructed without additionally adding inorganic strong acid or chemical additives; the problems of large discharge amount of acid wastewater, harsh reaction conditions and serious secondary pollution in the traditional technology are fundamentally solved, and a brand new technical path is provided for green and low-carbon resource utilization of the waste LIBs.

This invention discloses a method for efficiently leaching manganese from pyrolusite using acidophilic leaching microorganisms, relating to the fields of hydrometallurgy and biometallurgy. The method involves leaching pyrolusite using acidophilic leaching microorganisms, supplemented with pyrite as an auxiliary iron-sulfur source to enhance the leaching process. The initial pH of the leaching medium is 1.6, the pulp concentration is 120 g / L, and the inoculum concentration of the acidophilic leaching microorganisms is 10 × 10⁻⁶. 8 Under conditions of pyrolusite / pyrite mass ratio of 2:1 (cells / mL), the manganese leaching rate can reach 98.2% after 16 days of bioleaching. This invention significantly improves manganese leaching efficiency compared to a system without pyrite addition (3.2%). This invention helps solve the technical problems of high energy consumption and significant pollution in traditional pyrometallurgical and hydrometallurgical processes, and has advantages such as high leaching efficiency, environmental friendliness, low cost, and simple process. It is suitable for the green development and utilization of pyrolusite, especially low-grade pyrolusite resources.

The invention provides a biological leaching system and method for low-grade ore or smelting slag, and the system comprises a biological leaching breeding device which comprises a reactor, an upward lifting type multi-layer stirring assembly and an inclined blowing type aeration assembly; the stirring end of the lifting type multi-layer stirring assembly is vertically arranged in the reactor, the lifting type multi-layer stirring assembly comprises a first stirring paddle and a second stirring paddle, and the first stirring paddle is arranged in the middle of the reactor and used for preventing secondary sedimentation of fine particle slag; the second stirring paddle is arranged at the bottom of the reactor, and paddles of the second stirring paddle form a lifting type V shape and are used for lifting and mixing the whole material; the first stirring paddle and the second stirring paddle are different in rotating speed and are used for generating vertically upward lifting force to maintain the suspension state of the slag; the multiple bacterium liquid storage tanks are sequentially connected in series and used for storing culture solutions of different stages and / or different strains, and liquid outlets in the tail ends of the bacterium liquid storage tanks are communicated with the bottom of the reactor; according to the invention, the mass transfer and strain growth environment can be improved, and the precise control of the bacterium liquid adding and leaching process is realized.

The invention belongs to the technical field of soil heavy metal, and particularly discloses a method for repairing heavy metal contaminated soil through combination of bioleaching and biological vulcanization. The method comprises the following steps: S1, adding agricultural and forestry wastes and elemental sulfur and / or pyrite into the heavy metal contaminated soil, inoculating bacteria for producing organic acids and acidophilic iron-sulfur oxidizing bacteria, and carrying out domestication culture to obtain a composite flora after domestication culture; s2, the original heavy metal contaminated soil is utilized to enrich sulfate reducing bacteria composite flora under the anaerobic condition; s3, inoculating the composite flora subjected to domestication culture obtained in S1 into heavy metal contaminated soil for bioleaching; s4, carrying out solid-liquid separation on the leached soil, and adjusting the pH value of the system to be neutral; and S5, inoculating the sulfate reducing bacteria composite flora obtained in the step S2 into the soil treated in the step S4, and carrying out biological vulcanization. The method is high in heavy metal removal and fixation efficiency, the remediation depth reaches the standard, and the physical and chemical properties and fertility of the soil can be effectively protected.

The present invention relates to a process of releasing metallics, metalloids and / or non-metallics and / or degradating organics by bioleaching out of a carrier material, such as minerals, rocks, wastes, soil, and sediments. Furthermore, the present invention relates to a leached formulation, obtainable form said process, and to the use of said leached formulation as a biofertilizer or biostimulant or biopestizide.

The invention relates to the technical field of low-grade mineral resource bioleaching and chemical crystallization, in particular to a tailing bioleaching circulating liquid magnesium ion concentration control and magnesium recycling system which comprises a circulating liquid monitoring unit, an extraction impurity removal unit, an evaporation concentration magnetization crystallization unit and a recycling unit. All the units work cooperatively to realize magnesium ion concentration regulation and resource utilization; the concentration of Mg < 2 + > in the circulating liquid is stably controlled to be 25 g / L or below through the circulating liquid monitoring unit and flow division control, the activity of mineral leaching bacteria is guaranteed, and the leaching efficiency is prevented from being reduced; the extraction and impurity removal unit selectively removes impurities, and is matched with the evaporation, concentration and magnetization crystallization unit (enhanced by waste heat and a magnetic field), the crystallization efficiency is improved by 20% or above compared with that of a traditional process, the recovery rate of magnesium sulfate crystals reaches 35%-64%, and the purity is larger than or equal to 99.5%; the organic phase and water resources are recycled, energy consumption is reduced through waste heat, the energy consumption is integrally saved by 15% or above, and magnesium resource utilization is achieved.

This invention discloses a method for enhancing the bioleaching of arsenopyrite with bio-derived Schehertz minerals and simultaneously fixing arsenic, belonging to the fields of biometallurgy and mine environmental remediation technology. The method involves acclimating *Thiobacillus acidophilus* to arsenic tolerance in a culture medium containing arsenopyrite, then using the resulting arsenic-tolerant acclimation bacterial solution to biosynthesize Schehertz minerals. The synthesized Schehertz minerals are then uniformly mixed with the target arsenopyrite, and the arsenic-tolerant acclimation bacterial solution is added to initiate a bioleaching reaction, achieving in-situ solidification of arsenic. This method utilizes the strong adsorption capacity of the bio-derived Schehertz minerals for liquid-phase arsenic ions to rapidly reduce the concentration of free arsenic, thereby relieving the toxic inhibitory effect of arsenic on acidophilic microorganisms and shortening the microbial adaptation period. Simultaneously, the Schehertz minerals partially dissolve and release Fe in an acidic leaching environment. 3+ With As 5+ Co-precipitation forms ferric arsenate minerals, achieving in-situ stabilization and fixation of arsenic.