51 results about "Bioleaching" patented technology

The invention discloses a method for preparing ultra-high-purity quartz by multi-stage synergistic purification of quartz ore, and belongs to the technical field of ultra-high-purity quartz preparation. According to the method, natural quartz ore is used as a raw material, mineral impurities, lattice impurities and inclusions in quartz are deeply removed through multi-stage synergistic processes such as low-temperature plasma activation, biological leaching, supercritical CO2-assisted acid leaching, microwave-assisted chloridizing roasting and pulsed electric field enhanced acid leaching, and finally a high-purity quartz product with the SiO2 purity larger than or equal to 99.998% (4N8 grade) is obtained. According to the invention, a low-temperature plasma activation technology, microbial metallurgy, supercritical fluid extraction and a pulsed electric field strengthening technology are organically combined, so that the energy efficiency bottleneck and environmental protection limitation of a traditional high-purity quartz preparation process are broken through; the obtained quartz sand product can reach the standards of high-purity quartz sand for semiconductor quartz glass and high-purity quartz sand for photovoltaic quartz crucibles.

The present invention discloses a method for recovering zinc, lead, and silver from zinc-oxygen press slag using a combination of microbial leaching and chloride leaching. This method simultaneously recovers zinc, lead, and silver from zinc-oxygen press slag through a process of crushing, slurry adjustment, acid balancing, bioleaching, solid-liquid separation, slurry adjustment, chloride leaching, and solid-liquid separation. The leaching rates for zinc, lead, and silver are ≥95%, ≥95%, and ≥90%, respectively. This method offers the advantages of simple process, environmental friendliness, ease of operation, and high recovery of valuable metals.

The invention relates to a method for regulating and deacidifying in a bioleaching process of low-grade copper sulphide ore, aiming at the problem of excessive acid caused by excessive oxidation of pyrite in a dump leaching system, a thiocyanate bactericide is added into the bioleaching system of the low-grade copper sulphide ore, and the thiocyanate bactericide is dissolved in a spray liquid of the bioleaching system, and is added into the spray liquid of the bioleaching system; under the catalytic action of mineral leaching microorganisms, oxidation of ferrous ions is enhanced, so that the oxidation-reduction potential of a solution is increased, oxidation of pyrite is intensified, acid accumulation is excessive, the thiocyanate bactericide can remarkably inhibit the activity of part of sulfur-oxidizing bacteria and even kill part of sulfur-oxidizing bacteria, and therefore the activity of ferrous oxide is reduced; compared with the prior art, the method has the advantages that the sulfur oxidation activity of microorganisms is greatly influenced, but the oxidation influence on iron is relatively small, so that the acid leaching is reduced under the condition that the copper leaching of the copper sulfide ore is not influenced, the copper leaching is not influenced, the acid leaching is reduced, the production cost is reduced, the environmental influence is reduced, and the like.

The invention discloses a method for removing heavy metals in sludge, and belongs to the field of environmental protection technology and solid waste recycling. The method comprises the following steps: adding a bioleaching bacteria solution and a nutritive salt solution into dewatered sludge, and adjusting the pH value to form mixed slurry; applying an asymmetric alternating-current electric field to the mixed slurry to perform biological-electrochemical coupling treatment, and performing solid-liquid separation on the treated slurry to respectively obtain purified sludge and leachate containing heavy metals. By utilizing polarization inhibition and oscillation mass transfer effects of the asymmetric alternating-current electric field, the removal efficiency of the bioleaching on the heavy metals in the sludge, especially the organic combined state and residual state heavy metals, is remarkably enhanced, and the method has the outstanding advantages of short treatment period, low energy consumption, no electrode polarization and high heavy metal removal rate.

The invention relates to the technical field of solid waste biological treatment, and discloses La and Ce toxicity resistant thiobacillus thiooxidans and application thereof, and the collection number of the thiobacillus thiooxidans is GDMCC No: 63522. The invention provides a novel thiobacillus thiooxidans, and the preservation number of the novel thiobacillus thiooxidans is GDMCC No: 63522. The strain can highly tolerate La and Ce, and can realize biological leaching of solid wastes rich in La or (and) Ce under high ore pulp density, so that more solid wastes can be treated in a single batch, the biological leaching rate is improved, and the strain has a good application prospect.

The present application relates to the technical field of health, environmental management and resource development, and discloses an alkaline-sulfur oxidizing bacteria and its application in water pollution treatment and bioleaching. The alkaline-sulfur oxidizing bacteria is named 381-2, which has been preserved in the China General Microbiological Culture Collection Center on December 23, 2024, and the preservation number is CGMCC No. 33017; the microbial classification name is Stutzerimonas sp. The present application isolates a new species of Stizolobium strain from a deep-sea hydrothermal area Stutzerimonas sp. . The strain has strong and rapid oxidation ability of thiosulfate ions, and also has the characteristics of producing alkali, so it can be applied to the fields of water pollution treatment and bioleaching.

A bioleaching reactor for removing heavy metals in industrial sludge relates to the technical field of environmental protection equipment, and is characterized in that a venturi mixer is fixedly arranged on the left side of the upper part of a reaction cylinder, and two liquid inlets of the venturi mixer are respectively connected with a sludge conveying pipe and a bacterial liquid conveying pipe; a material guide frame is fixedly arranged at the position, located above the solid-liquid separation assembly, in the reaction cylinder, the motor is fixedly arranged on the upper portion of the reaction cylinder, a stirring shaft is rotationally arranged on the top wall in the reaction cylinder through a bearing, a plurality of stirring blades are connected to a connecting disc through a self-adaptive adjusting mechanism, and spiral propelling pieces are fixedly arranged on a plurality of connecting rods. The stirring shaft is provided with a paddle at the position on the other side of the connecting rod, so that the three-dimensional efficient mixing of the sludge and the bacterial liquid can be realized, the dead angle of mixing is reduced, the angle of the stirring blade can be automatically adjusted through the centrifugal force or resistance during stirring, the shearing force on the high-viscosity sludge is enhanced, the good mixing effect between the sludge and the bacterial liquid can be achieved, and the stirring efficiency is improved. The reaction efficiency is improved.

The present invention relates to a method for preparing concrete using bioleached residues of cobalt-molybdenum-based waste catalysts. The method involves mixing the bioleached residues, cement, standard sand, and water, stirring the mixture, performing a hydration reaction, and curing the resulting concrete. The advantages of this method are that the bioleached residues of cobalt-molybdenum-based waste catalysts, classified as HW50 hazardous waste, are used as a concrete admixture, reducing cement usage, improving concrete performance, and lowering production costs, thereby achieving resource utilization of waste.

The present invention relates to a method for recovering metals from waste batteries by using a magnetic field-applied bioleaching process. According to the present invention, by applying a magnetic field to a bioleaching process, it is possible to improve the recovery rate of metals, and to leach valuable metals. In addition, the method is environmentally friendly compared to a dry melting process or an acid leaching process, which has been mainly used for metal recovery, and is practical due to a simple process thereof. Therefore, the method for recovering metals of the present invention may recover metals from wastes in waste resources field such as waste batteries and waste catalysts and turn the metals into resources.

The invention relates to the technical field of sanitation, environmental governance and resource development, and discloses an alkali-producing sulfur oxidizing bacterium and application thereof in water pollution governance and bioleaching. The alkali-producing sulfur-oxidizing bacterium is named as 381-2, is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 23, 2024, and has a preservation number of CGMCC No.33017; the microbial classification is named as Stutzerimonas sp., and the microbial classification is named as Stutzerimonas sp. According to the invention, a new species strain Stutzerimonas sp. Of Stutzerimonas sp. Is obtained by separation from a deep sea hydrothermal zone. The bacterial strain has relatively strong capability of rapidly oxidizing thiosulfate ions and also has alkali production characteristic, so that the bacterial strain can be applied to the fields of water pollution treatment and bioleaching.

The application discloses a method for bioleaching copper and zinc in lead-zinc smelting slag, which comprises adding the lead-zinc smelting slag into an acid culture medium inoculated with acidophilic leaching bacteria domesticated under metal salt stress for a certain period of time. Compared with the prior art, the acidophilic leaching bacteria domesticated under high-concentration metal salt stress can realize efficient leaching of copper and zinc in low-grade lead-zinc smelting slag, realizes waste-to-resource, and converts the original environmental burden into valuable resources, which conforms to the concept of sustainable development and circular economy, and has the advantages of environmental friendliness and low operation cost.

The invention relates to a microorganism-based ionic rare earth ore extraction method in the technical field of rare earth extraction, and the method comprises the following steps: step 1, culturing a microorganism Burkholderia contaminans GP4-1 to obtain an ore leaching bacterial liquid containing microorganisms and metabolites thereof; secondly, the ore leaching bacterial liquid prepared in the first step makes contact with ionic rare earth ore, and a biological leaching reaction is carried out; and thirdly, a leaching mother solution rich in rare earth elements is collected. By means of the method, selective enrichment of heavy rare earth can be achieved.

The application provides an extremely acidophilic iron-sulfur oxidizing bacterium and application thereof in bioleaching of ores. The iron-sulfur oxidizing bacterium (Sulfobacillus thermotolerants) hq2 strain has a preservation number of CGMCC No. 26454. The iron-sulfur oxidizing bacterium provided by the application can oxidize Fe 2+ into Fe 3+ , and can be used for leaching of various low-grade ores and other heavy metals.

This application provides a bioleaching method for low-grade copper ore, comprising the following steps: crushing copper-bearing ore and pyrite separately, screening to obtain copper ore powder and iron ore powder, mixing the copper ore powder and iron ore powder and piling them in a heap leaching column, and burying vertical pipes in the heap to obtain a heap; spraying the heap with acid, then inoculating with leaching bacteria solution to carry out the heap leaching process; the leaching process uses raffinate for circulating leaching. The method of this application uses bioleaching to treat low-grade copper ore, which has the advantages of high leaching rate, low pollution, water conservation, and ease of operation. It overcomes the disadvantages of traditional flotation methods for separating low-grade copper ore, which are not only difficult to process but also have extremely low flotation yields, making it difficult to obtain high-grade copper concentrate.

The present application relates to a kind of vacuum roasting coupling microbial upgrading no-bonding high-sulfur bituminous coal preparation silicon smelting reducing agent method, belong to industrial silicon smelting composite reducing agent technical field.The present application mixes even with bituminous coal powder and corn cob powder and obtains mixture A, hydrogen peroxide solution is added in mixture A and grinds and mixes evenly and obtains mixture B;Mixture B is placed in temperature 800~1200 ℃ under vacuum roasting 30~90min, with furnace cooling and obtains roasting mixture C;Deionized water, bacteria liquid and sodium silicate are added to roasting mixture C, and carry out microbial leaching at temperature 25~65 ℃ for 5~10h, and stratify after standing, remove supernatant;Repeat microbial leaching step until supernatant is neutral, solid-liquid separation, and solid drying obtains low-sulfur composite reducing agent.The low-sulfur composite reducing agent obtained by the present application reaches 91.2~93.7%, and the caking index is 28~53, and the reaction activation energy is 13.4~17.7KJ / mol, meet the requirements of industrial silicon composite reducing agent.

The invention discloses a method for determining lithium in a lithium ore bioleaching solution, and belongs to the technical field of biological metallurgy, the determination method comprises the following steps: preparing a lanthanum nitrate-strontium chloride composite matrix improver, sampling from the lithium ore bioleaching system leaching solution, centrifuging to remove solid impurities, filtering the solid impurities by using a 0.22 [mu] m filter membrane, and collecting the lithium in the lithium ore bioleaching system leaching solution; a sample is diluted for standby application, a lanthanum nitrate-strontium chloride composite matrix improver is added into the sample, the concentration ratio of lanthanum nitrate to strontium chloride in the lanthanum nitrate-strontium chloride composite matrix improver is 1: 2, and the sample is measured by adopting a flame atomic absorption spectrophotometer. The method is especially suitable for bioleaching solution analysis of lithium ore and efficient detection of lithium resources, the inhibition effect of coexisting ions on lithium atomization is avoided through the lanthanum nitrate-strontium chloride composite matrix improver, and the coexisting interfering ions are competitively complexed, background absorption is reduced, and a lithium signal is prevented from being covered, so that the determination accuracy is improved.

An oxidative bioleaching process for leaching a base metal from an ore that includes an ore agglomeration step, an ore stacking step wherein agglomerated ore is stacked to form a heap, a curing step, a rinse step, an inoculation step and a leach step, and wherein, during the ore agglomeration step, the ore is contacted with an acid solution containing nitrate or nitrite thereby to accelerate the leaching rate in the leach step.

The invention discloses a method for biologically leaching lithium in lithium-containing silicate ore by using amino acid, which belongs to the technical field of biological metallurgy, and comprises the following steps: pretreating lepidolite to obtain lepidolite mineral powder, culturing an ore leaching strain in a culture medium, adding the lepidolite mineral powder for domestication, centrifuging a leaching solution, and collecting the domesticated ore leaching strain, thereby obtaining the lithium-containing silicate ore. Preparing a biological leaching system, inoculating the domesticated mineral leaching strain into the biological leaching system, adding glycine, and leaching to obtain a leaching solution. The method is simple in required equipment, mild in reaction condition, low in cost and environment-friendly, and glycine is low in price and easy to obtain.

This invention discloses a method for improving the bioleaching efficiency of heavy metal contaminated soil using a carbon rod electrode system modified with graphene oxide (GO). The invention involves preparing a carbon nanomaterial, GN-GO, from traditional graphene rod electrodes using a GO suspension through a multiple soaking and drying process. The GN-GO material is then used as the anode, and the unmodified graphene rod electrode as the cathode. The anode and cathode, connected to an external power source via wires, are placed in a container containing a culture medium and a heavy metal contaminated soil sample. An acclimatized bacterial strain is then introduced, and leaching is performed at 25–30°C and 120–150 r / min. This invention improves the conductivity and specific surface area of ​​the electrode by altering the electrode surface structure of the bioelectrochemical system, while simultaneously optimizing the biocompatibility of the electrode material. The method combines electrochemical and biological approaches, resulting in high remediation efficiency and good remediation effects for heavy metal contaminated soil.

This invention relates to the field of environmental engineering technology and discloses a method for treating wastewater containing copper nitrate and copper sulfate from the high-purity copper industry. The method involves filtering the copper nitrate and copper sulfate wastewater into reaction tanks A and B respectively, adding flocculants, monitoring the pH value of the reaction tanks during the reaction process using online monitoring instruments, and separately adding hydrogen peroxide to reaction tank A to break down the complexed copper and convert it into Cu. 2+ The supernatant is used as effluent and treated by diffusion dialysis. Activated carbon adsorption and reverse osmosis separation are used to further remove residual copper ions. The sludge is washed eight times with circulating water. Based on sludge stabilization, the sludge is converted into organic fertilizer through composting. Bioleaching technology uses microorganisms or their metabolites to leach valuable metals from the sludge through interaction with the metals. The treated wastewater is neutralized and then discharged into a comprehensive wastewater pond. The recovered copper is electrolyzed to produce copper products with 98% purity.

The present application relates to a kind of copper in waste paint residue resource recovery method, belong to solid waste resource processing technical field.The method is as follows: waste paint residue is broken and ground;In inorganic salt culture medium, biological leaching bacterial strain is obtained by shaking table culture biological leaching liquor;When biological leaching liquor pH drops to 0.6-1.4, according to 2% to 10% solid-liquid ratio, paint residue powder is input, and shaking table culture is carried out until the dissolution concentration of copper ion no longer increases, and biological leaching ends.Biological leaching liquor is extracted in the extractant formed by LIX984 and sulfonated kerosene DT100, and the oil phase and water phase enriched in copper are separated;The oil phase is back-extracted with sulfuric acid solution, and the water phase and oil phase enriched in copper are separated;Water phase is evaporated and cooled to crystallize and obtain copper sulfate crystal.This method recycles copper ions in waste paint residue, with the characteristics of high recovery rate, simple process, energy saving and environmental protection.

This invention relates to a method for separating and recovering arsenic and iron from arsenic- and iron-containing bioleaches. The specific steps are as follows: An adsorbent is added to the arsenic- and iron-containing bioleach, mixed thoroughly, and reacted. After adsorption is complete, the solid and liquid phases are separated to obtain an arsenic-iron solution free of bacterial residues. A reducing agent is added to the obtained arsenic-iron solution. After detection, all arsenic and iron are converted to trivalent and divalent valences, respectively, and the reaction is complete, yielding a solution containing low-valent arsenic and low-valent iron. An extractant is added to the reduced arsenic-iron solution to obtain an arsenic-containing organic phase and an iron-containing raffinate. The iron-containing raffinate is returned to the bioleaching process, and the organic phase is back-extracted to obtain an arsenic-containing back-extract. The back-extracted organic phase is reused to extract arsenic. Sodium hydroxide is added to the arsenic-containing back-extract, mixed thoroughly, and then benzyl chloride is added. After stirring and reacting, the mixture is allowed to stand for layering and the acidity is adjusted for crystallization. The crystals are washed and dried to obtain benzylarsine product. This invention solves the shortcomings of existing neutralization processes, such as large slag volume, difficulty in product purification, complex processes, and low arsenic and iron recovery rates. It fully utilizes the arsenic and iron resources in the bioleaching solution and has the advantages of high recovery rate, high product added value, no secondary pollution generated in the process, and low investment.

This invention provides a method for using enzymes to cyanide a metal and reduce the metal-cyanide complex, as well as a method for using biological techniques to hydrolyze cyanide. [Solution] The present invention relates to the use of genetically modified bacteria in a process comprising the generation of cyanide, the reduction of leached metal ions, the decomposition of cyanide, and the subsequent reuse of cyanide. Furthermore, it provides a transcriptional regulatory tool in Chromobacterium violaseum. In one embodiment, the present invention provides a genetically modified bacterium that is isolated and transformed by at least one polynucleotide molecule, wherein the polynucleotide molecule comprises a heterologous mercury(II) reductase (MerA) gene operably linked to at least one promoter, and comprises at least one mutation in which a metal ion is reduced by the gene product of the MerA gene to produce a metal element as metal nanoparticles.

This invention provides a method for recovering metallic copper by atmospheric pressure oxygen-enriched leaching, relating to the field of hydrometallurgy. The method includes: mixing copper sulfide concentrate with a sulfuric acid solution to obtain a mixed slurry; introducing an oxygen-rich gas under atmospheric pressure to carry out a leaching reaction; and after the reaction, separating the liquid and solid to obtain a copper-containing leachate. By using the synergistic leaching of sulfuric acid solution and oxygen-rich gas under atmospheric pressure, this method eliminates the reliance on expensive high-pressure equipment, removes the safety hazards of high-pressure explosions, and significantly reduces equipment corrosion and investment costs. Using oxygen as a highly efficient oxidant, this method significantly shortens the extraction cycle, overcomes the drawbacks of time-consuming bioleaching and the generation of waste, and achieves efficient, safe, and low-cost extraction of metallic copper from copper sulfide concentrate.

A coupled beneficiation method for gold-bearing copper ores with a high sulfur-to-copper ratio employs the SABC process. The ore is graded by semi-autogenous grinding and ball milling, copper is enriched by bioleaching using semi-autogenous grinding stone, coarse gold in the crushed product is selectively enriched by Nelson, copper is separated by Nelson tailings using differential speed asynchronous separation, fine gold is enriched into copper concentrate using a gold-loaded sulfide collector BY-19 coupled with a low-alkalinity inhibitor BY-23, and the sulfur concentrate is recovered by microbubble flotation using a BY-80 collector, thereby improving the recoveries of gold, copper, and sulfur. Specifically, the method comprises the following steps: semi-autogenous grinding and ball milling for classification, copper is enriched by bioleaching using semi-autogenous grinding stone, coarse gold in the crushed product is selectively enriched by Nelson, copper is separated by Nelson tailings using differential speed asynchronous separation, fine gold is enriched into copper concentrate using a gold-loaded sulfide collector BY-19 coupled with a low-alkalinity inhibitor BY-23, and sulfur concentrate is recovered by microbubble flotation using a BY-80 collector. The method has the advantages of significantly improving the recovery rate of copper concentrate Cu, avoiding the loss of valuable metals, being beneficial to improving process indicators, maximizing the comprehensive recovery rate of gold, and improving the gold recovery rate and sulfur recovery rate under the condition of consistent copper selection indicators.

The invention relates to a method for separating and recycling rare earth and a leaching agent in rare earth-containing bioleaching liquid, which is characterized by comprising the following steps: 1) obtaining the bioleaching liquid rich in rare earth; 2) removing impurities; 3) activating a coordination chemical bond; 4) activating the transformation material; (5) fracture and reconstruction of coordinate bonds; and 6) cyclic utilization. Based on the principles of activation, fracture, reconstruction and the like of a bioleaching agent-rare earth coordination chemical bond, rare earth elements in the bioleaching agent can be efficiently separated and recovered (the rare earth recovery rate is close to 100%) in a transformation separation mode; in the rare earth separation and recovery link, a bioleaching agent-rare earth coordination chemical bond is broken, transformation of a rare earth bioleaching agent and selective separation of rare earth elements are achieved, the bioleaching agent is reserved in a solution and can be used for subsequent leaching of rare earth raw materials, and recycling of the bioleaching agent is achieved (the regeneration rate is close to 100%); chemical reagents do not need to be added, so that introduction of environmental pollution substances is avoided; the method is simple in equipment and operation, good in effect, low in cost, environment-friendly and convenient for large-scale industrial application.

The invention discloses a method for recycling copper and zinc from high-iron low-grade copper-zinc ore through biological leaching and application, and relates to the technical field of comprehensive utilization of mineral resources. The method for recycling copper and zinc through biological leaching of the high-iron low-grade copper-zinc ore comprises the steps that a mixed flora containing ferrous oxide thiobacillus, thiobacillus thiooxidans, leptospirillum ferriphilum and acidithiobacillus caldus is domesticated, and ore leaching bacterial liquid is obtained; the method comprises the following steps: carrying out biological leaching treatment on a mixture of high-iron low-grade copper-zinc ores by adopting an ore leaching bacterial solution, reducing Fe < 3 + > in a leaching solution into Fe < 2 + >, removing iron in a reducing solution by combining a potential-controlled goethite method, and finally, sequentially recovering copper and zinc. The method can realize efficient recovery of copper and zinc, and has the advantages of environmental protection and low cost.

The invention provides a low-grade copper ore bioleaching method which comprises the following steps: respectively crushing and sieving copper-containing raw ore and pyrite to obtain copper ore powder and iron ore powder, mixing the copper ore powder and the iron ore powder, stacking in a dump leaching column, and burying a vertical pipe in an ore heap to obtain the ore heap; spraying the ore heap with an acid solution, then inoculating the ore leaching bacteria solution, and carrying out a heap leaching process; raffinate is adopted for cyclic leaching operation in the leaching heap process. According to the method, the biological leaching method is adopted for treating the low-grade copper ore, the method has the advantages of being high in leaching rate, small in pollution, capable of saving water resources and easy to operate, and the defects that when the low-grade copper ore is separated through a traditional flotation method, the treatment difficulty is large, the flotation income is extremely low, and high-grade copper concentrate is difficult to obtain are overcome.

The invention belongs to the technical field of resource utilization, and discloses a resourceful treatment method of manganese slag, which comprises the following steps: carrying out oxidation pretreatment on the manganese slag to obtain solid-phase residue and filtrate I; the solid-phase residues are subjected to reduction leaching, and reduction leaching residues and filtrate II are obtained; the reduction leaching residues are subjected to weak acid elution, and elution residues and filtrate III are obtained; the elution residues are subjected to biological leaching, and biological leaching residues and filtrate IV are obtained; and combining the lixivium in each stage, and purifying to obtain a manganese-rich solution. According to the method, manganese in different occurrence forms is treated in a step-by-step and targeted manner through a multi-stage sequential elution process of manganese slag oxidation pretreatment, reduction leaching and chemical-biological combined fine washing, and the total recovery rate of manganese is remarkably increased.

The present application belongs to the field of hydrometallurgy, and relates to a method for modifying biomass to strengthen bioleaching of ion-adsorbed rare earth ore, which is carried out according to the following steps: modifying biomass with a sulfuric acid solution with a mass concentration of 40% to 80% to obtain a modified biomass solution; mixing the modified biomass solution with Czapek's liquid medium to obtain a mixed culture solution; inoculating the mixed culture solution with mixed microbial strains to obtain a fermentation solution; mixing the fermentation solution with ion-adsorbed rare earth ore, stirring, filtering, and obtaining the supernatant as a rare earth leaching solution. The present application uses the modified biomass solution to provide a nutrient medium and a fermentation substrate for microorganisms, improves the activity of the microorganisms and the yield of organic acids, and has the characteristics of low cost, wide raw material sources, and small environmental pollution.