Method for leaching metal from metallic ores and special strain in same

A metal and ore technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of bacterial inactivation, loss of vitality, pH value reduction, etc.

Active Publication Date: 2010-08-04
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in practical applications, the heat generated by mineral oxidation often leads to higher temperatures in the leaching tank and leaching heap. If common ore leaching mesophilic bacteria are used for leaching, the bacteria will be inactivated due to the high temperature, resulting in The disadvantages of slow leaching speed and low

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  • Method for leaching metal from metallic ores and special strain in same
  • Method for leaching metal from metallic ores and special strain in same
  • Method for leaching metal from metallic ores and special strain in same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Isolation and identification of bacteria

[0045] 1. Separation

[0046] The strain of the present invention is obtained by separation and purification from the sulfur-rich hot spring mud sample in Tengchong County, Yunnan Province, China, and the separation and purification time is June 6, 2008.

[0047] The enrichment of microorganisms is carried out by adding 100 mL of basal medium to a 250 mL shake flask, weighing 5 grams of hot spring mud sample into the sterile basal medium, adding 5-10 g of sulfur, and enriching culture at 65°C. After one week of cultivation, check the growth of bacteria under a microscope. After enrichment for three times, dilute the enrichment medium 10 times, take 0.2mL of the diluted medium and spread it on a plate to separate the single clones, and add 7g / L Gelrite gum to the solid medium on the basis of the liquid medium to use tetrathionic acid Potassium replaces elemental sulfur as energy. Through multiple streaking cultures, a mon...

Embodiment 2

[0067] Example 2: Cultivation of bacteria

[0068] 1. Training method

[0069] (1) Autotrophic cultivation

[0070] Composition of autotrophic medium: FeSO was added to the basic medium separately 4 ·7H 2 O or elemental sulfur or reduced sulfide K 2 S 4 O 6 , Get autotrophic medium; FeSO 4 ·7H 2 The concentration of O in the autotrophic medium is 13.9g / L, the concentration of elemental sulfur in the autotrophic medium is 5g / L, and the reduced sulfide K 2 S 4 O 6 The concentration in the autotrophic medium is 10g / L; the pH is adjusted to 2.0-3.5.

[0071] Cultivation method: inoculate the acidophilus thermophilus (Metallosphaera sp.) Ar-4CGMCC No.3402 in an autotrophic medium and cultivate it at 65°C.

[0072] (2) Heterotrophic culture

[0073] Heterotrophic medium composition: adding yeast extract to the basic medium, the ratio of the yeast extract to the basic medium is 1g:1L; adjusting the pH value to 2.5-3.5, and autoclaving. Among them, yeast extract is an energy substance.

[0074] ...

Embodiment 3

[0082] Example 3. Application of bacteria

[0083] Yeast extract was purchased from Oxoid Ltd, the product catalog number is 1039501;

[0084] 1. Study on the ability to dissolve pyrite of Metallosphaera sp. (Metallosphaera sp.) Ar-4CGMCC No.3402

[0085] Metallosphaera sp. (Metallosphaera sp.)Ar-4CGMCC No.3402 extracts total iron (Fe) from pyrite 2+ And Fe 3+ ).

[0086] The ore composition of pyrite: S 45.57%, Fe 42.63%, Cu 0.063%, Zn 0.0076%, As 0.02%:% represents the mass percentage; the source of pyrite: Inner Mongolia Huogeqi Copper Mine.

[0087] Before the experiment, pass the pyrite through a 300-mesh sieve (the average particle size is 48μm), and then use 1:1H 2 SO 4 The aqueous solution (the concentration of sulfuric acid in the sulfuric acid aqueous solution is 50%, v / v) is soaked for 3-5 days, until the pH value of the acidified solution remains unchanged for 24 hours between 1.5-1.6.

[0088] Cultivate the acidophilus thermophilus (Metallosphaera sp.) Ar-4CGMCC No.3402 with...

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Abstract

The invention discloses a method for leaching metal from metallic ores and a special strain in the same. The strain is metallosphaera sp. Ar-4, with the collection number being CGMCC NO.3402. Experiments prove that the strain can grow under the environment of low pH value, high temperature and hypersalinity, can be used for leaching copper ions from the copper pyrite, with leaching rate of 10.6%, and can be also used for leaching iron ions from the pyrite (sulfur concentrate), with leaching rate of 2.9%. The high temperature ore-leaching strain improves the oxidation efficiency of the sulfide from at least two aspects as follows: first, with temperature rise, the reaction rate is increased; and second, temperature rise can expand the range of extraction of metal from a few minerals, thus marking up for the defects of unsuccessful leaching of a few minerals by the mesophilic strain, high investment, low efficiency and the like. The strain is especially suitable for the minerals with low leaching efficiency, such as copper pyrite. In addition, the strain has superstrong acid resistance; therefore, generated acidity can not affect the effects of the strain in the bioleaching process and can further improve the leaching efficiency.

Description

Technical field [0001] The invention relates to a method for leaching metal from metal ore and a special strain thereof. Background technique [0002] Biological hydrometallurgy is also called microbial leaching, which uses the oxidation, reduction, dissolution, absorption (adsorption) and other effects of certain microorganisms or their metabolites on certain minerals (mainly sulfide minerals) and elements to dissolve from ore The hydrometallurgical process of immersing metals or recovering valuable metals from water or removing harmful metals. Its development has a history of several decades. People are paying more and more attention due to its low cost, pollution-free, simple operation and other favorable factors. It has become a significant economic and environmental protection benefit for extracting a variety of useful metals from low-grade, refractory ores. The advanced technology of the company has been promoted, improved and improved worldwide. [0003] However, in practi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C22B3/18C12R1/01
Inventor 刘双江姜成英刘丽君郭旭尤晓颜王保军刘志培
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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