The invention discloses sgRNA used for targeted knockout of a human NKG2A/KLRC1 gene, and belongs to the technical field of genetic engineering. The sgRNA has an arbitrary nucleotide sequence as shownin SEQ ID NO.1-56. The invention further discloses a sgRNA compound, expression vector, CRISPR/Cas9 system and kit used for targeted knockout of the human NKG2A/KLRC1 gene, and application of the sgRNA compound in preparing an immune cell medicine for treating neoplastic diseases. By means of the prepared specific targeted human KNG2A/KLRC1 gene, the human NKG2A/KLRC1 gene can be precisely targeted, effective knockout can be achieved, and meanwhile, an obtained human NKG2A/KLRC1 gene knockout immune cell is suitable for multiple tumor cell models; and a preparation method has simple steps, the targeting specificity of the sgRNA is good, and the knockout efficiency of the CRISPR/Cas9 system is high.