29 results about "Thiobacillus ferrooxidans" patented technology

The invention discloses a method for synergistically enhancing biological oxidation of arsenopyrite by utilizing symbiotic minerals, which particularly comprises the following steps: adding a certain amount of pyrite into an arsenopyrite biological oxidation system, and improving the quantity and activity of acidophilic thiobacillus ferrooxidans by utilizing chemical change of a solution generated by oxidation of the pyrite. The mineral leaching capability of the functional microorganisms is optimized; meanwhile, under the dual action of pyrite and microorganisms, the content of Fe < 3 + > in the solution is remarkably increased, and the Fe < 3 + > serves as a main oxidizing agent for oxidizing arsenopyrite; due to the difference between the surface potentials of the pyrite and the arsenopyrite, a galvanic couple effect is formed between minerals, the electron transfer efficiency between the arsenopyrite and the oxidizing agent is accelerated, and finally the purpose of improving the leaching rate of the arsenopyrite in a biological system is achieved; according to the method, the leaching rate of arsenic in an arsenopyrite biological oxidation system is increased by nearly 8 times at most, the reinforcing effect on arsenopyrite oxidation is remarkable, and meanwhile the method is simple in process, mild in condition, easy and convenient to operate and low in cost.

The invention discloses a water quality conditioning agent based on plant extracts and a preparation method of the water quality conditioning agent, and belongs to the technical field of aquaculture. The water quality conditioning agent is prepared from the following raw materials in parts by weight: 14 to 18 parts of fructus schisandrae chinensis extract, 4 to 5 parts of fructus mume extract, 10 to 13 parts of rhizoma coptidis extract, 8 to 10 parts of glucosamine, 5 to 8 parts of chitosan, 3 to 6 parts of nitrifying bacteria, 2 to 4 parts of thiobacillus thiooxidans and 3 to 6 parts of zeolite powder. Through a four-dimensional synergistic mechanism of'plant active ingredient-chitosan slow release-microbial immobilization-zeolite adsorption ', the pH value, ammonia nitrogen, nitrite, hydrogen sulfide and bottom mud COD in tail water can be reduced and the dissolved oxygen amount can be increased under the condition of one-time addition, which indicates that the water quality regulator has a good effect of improving water quality.

The invention relates to the technical field of regenerated rubber particle preparation, in particular to a preparation method of environment-friendly tire regenerated rubber particles. Comprising; immersing rubber powder into a swelling solution for swelling, and drying for later use; performing plasma activating treatment on the dried rubber powder in a plasma generator; culturing thiobacillus ferrooxidans to a logarithmic phase to obtain a free bacteria solution; fixing a part of the free bacterial liquid on magnetic nanoparticles, and modifying the surfaces of the magnetic nanoparticles with amino groups to obtain an immobilized bacterial liquid; the method comprises the following steps: mixing activated rubber powder with a free bacteria solution, carrying out a first-stage reaction, carrying out centrifugal separation on the rubber powder subjected to the first-stage reaction, putting into a second-stage reaction, putting the immobilized bacteria solution and the rubber powder into a reactor according to a ratio of 10L of bacteria solution to 1kg of rubber powder, and adopting a magnetically stabilized fluidized bed reactor, a built-in permanent magnet array and the like as the reactor; the one-flash scheme effectively balances the contradiction among the environmental protection property, the product performance and the reaction efficiency.

The invention belongs to the technical field of heavy metal contaminated soil bioremediation, and particularly relates to a method for remediating heavy metal contaminated soil through a Fe3C-N-S composite modified electrode enhanced bioelectrochemical system. Graphene oxide, an iron source, a nitrogen source and a sulfur source serve as raw materials, a Fe3C-N-S composite material is prepared through high-temperature calcination, then the Fe3C-N-S composite material is compounded with sodium alginate (SA) hydrogel, the surface of a graphite rod is coated with the composite material, a Fe3C-N-S composite modified electrode is constructed through a CaCl2 cross-linking and freeze-drying technology, then the modified electrode is coupled with a graphite cathode, and the composite material is obtained. And the acidophilic thiobacillus ferrooxidans is combined to construct a bioelectrochemical system, so that the synergistic and efficient leaching of the heavy metals in the soil is realized. The conductivity, biocompatibility and corrosion resistance of the electrode can be remarkably improved, the problems that mass transfer is difficult and microorganism loading is unstable in BES remediation are solved, and a breakthrough technical scheme is provided for green and efficient remediation of heavy metal contaminated soil.

The invention relates to the technical field of solid waste biological treatment, and discloses La and Ce toxicity resistant thiobacillus thiooxidans and application thereof, and the collection number of the thiobacillus thiooxidans is GDMCC No: 63522. The invention provides a novel thiobacillus thiooxidans, and the preservation number of the novel thiobacillus thiooxidans is GDMCC No: 63522. The strain can highly tolerate La and Ce, and can realize biological leaching of solid wastes rich in La or (and) Ce under high ore pulp density, so that more solid wastes can be treated in a single batch, the biological leaching rate is improved, and the strain has a good application prospect.

The invention relates to the technical field of biological metallurgy, in particular to an enhanced leaching method of jorusite. The enhanced leaching method of the jorlybdenum ore comprises the following steps: mixing a liquid phase system containing Fe < 2 + > and a bacterial liquid system formed by acidophilic thiobacillus ferrooxidans with nitrite to obtain a mixed system; then mixing with jorlybdenum ore to obtain a first system; adjusting the pH value of the first system by using an acid solution, and then carrying out oscillation treatment; the concentration of the nitrite in the mixed system is 40-60 mmol / L. According to the enhanced leaching method of the jorlybdenum ore, the complex metabolic process of acidophilic thiobacillus ferrooxidans is combined with nitrite enhanced biological leaching, nitric acid generated by metabolism of the acidophilic thiobacillus ferrooxidans provides acid for molybdenum dissolution of the jorlybdenum ore, generated Fe < 3 + > can be subjected to oxidation-reduction reaction with MoS2 to form stable complex ions [MoO4] < 2->, and the stable complex ions [MoO4] < 2-> are subjected to oxidation-reduction reaction with MoS2. The nitric acid can reinforce the dissolution and leaching of Mo from mineral jorlybdenum ore, so that the leaching effect of Mo is improved.

The invention relates to a metal leaching agent and a method for leaching and recovering metal from a retired photovoltaic module step by step. The metal leaching agent comprises an acidophilic thiobacillus ferrooxidans solution, a hidden acidophilic bacteria solution and a thiobacillus thiooxidans solution, the volume ratio of the acidophilic thiobacillus ferrooxidans liquid to the hidden acidophilic bacteria liquid to the thiobacillus thiooxidans liquid is (0.5-1.5): (0.5-1.5): (0.5-1.5); wherein the concentration of the acidophilic thiobacillus ferrooxidans, the acidophilic hidden bacteria and the thiobacillus thiooxidans is (1-9) * 10 < 8 > cells / mL. Furthermore, the invention provides a method for leaching and recovering metal from the retired photovoltaic module step by step. According to the method, the metal leaching agent is utilized, mild and green recovery of aluminum and silver in the decommissioned photovoltaic cell piece and full-process recovery of the decommissioned photovoltaic module can be achieved through step-by-step biological leaching, and the method has the excellent leaching effect on aluminum and silver.

The invention relates to the technical field of microbial metallurgy, in particular to a method for strengthening leaching of rare earth elements by rare earth-resistant biological acid flora through gradient stress domestication, which comprises the following steps: selecting an original strain of thiobacillus thiooxidans for ultraviolet radiation mutagenesis, and primarily screening mutant strains through a high-throughput screening condition; carrying out compound induction on the culture medium and carrying out four-stage stress domestication on the screened strains; selecting a composite carrier, and curing the domesticated bacterial liquid and the carrier; the industrial microbial inoculum is prepared based on microbial inoculum production and rare earth leaching processes. According to the method for strengthening leaching of the rare earth elements from the rare earth-resistant biological acid flora through gradient stress domestication, rare earth concentration gradient-pH gradient-temperature fluctuation-tailing matrix substitution is constructed as a gradient stress domestication stage, and a composite induction culture medium is combined to realize directed evolution of flora genomes, so that comprehensive improvement of stress resistance indexes is ensured; by designing a composite carrier structure and introducing an immobilization process, a carrier immobilization technology is realized to guarantee industrial stability.

The invention relates to a water regulating microbial preparation for breeding largemouth bass seedlings in a land-based round pond and application, and belongs to the technical field of aquaculture. The preparation aims to solve the problem of combined pollution caused by synchronous exceeding of ammonia nitrogen, nitrite and hydrogen sulfide in a water body due to accumulation of residual feed and excrement in a land-based round pool high-density breeding environment. According to the scheme, the microbial agent is prepared by physically mixing bacillus standard bacterial powder, photosynthetic bacteria standard bacterial powder, compound nitrifying bacteria standard bacterial powder and thiobacillus standard bacterial powder according to specific parts by weight, wherein the bacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis, and the thiobacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis. The composite nitrifying bacteria standard bacterial powder is formed by mixing nitrite bacteria and nitrifying bacteria, and the thiobacillus thiooxidans standard bacterial powder is formed by mixing thiobacillus thiooxidans and thiobacillus thioparus. The preparation is used for synchronously degrading ammonia nitrogen, nitrite and hydrogen sulfide in aquaculture water, so that normal development of gonads of largemouth bass parents is guaranteed, and the hatching rate of fertilized eggs and the survival rate of early-stage seedlings are increased.

The invention relates to a preparation method and application of a microbial fertilizer for preventing and treating citrus diseases, and belongs to the technical field of fertilizer.The microbial fertilizer comprises beneficial microorganisms such as thiobacillus thiooxidans, nitrogen-fixing bacteria and saccharomycetes, humic acid, an AMS-SiO2 carrier, biochemical potassium fulvate and mineral elements. The preparation method comprises the following three key steps: firstly, combining beneficial bacteria with humic acid through a microbial flocculation technology to form a flocculation carrier; secondly, immobilizing paecilomyces lilacinus, bacillus and trichoderma by using the porous AMS-SiO2 carrier to form a complex microbial inoculant; the application of the fertilizer aims at citrus diseases such as citrus huanglongbing, canker and anthracnose, an annular furrow application, broadcast application prevention and chemical pesticide synergistic scheme is adopted, growth period management is combined, and by optimizing the carrier structure and flora compatibility, the fertilizer can be applied to citrus diseases such as citrus huanglongbing, canker and anthracnose. The method solves the problems of low survival rate of microorganisms in a soil environment, disease prevention time mismatch and the like, and obviously improves the disease prevention and control effect and the fruit quality.

The invention relates to the technical field of hydrometallurgy, in particular to a method for enhancing microbial leaching of secondary copper sulphide ore of polymetallic ore by using a surfactant. The method for enhancing microbial leaching of the secondary copper sulphide ore of the polymetallic ore by using the surfactant comprises the following steps: S1, crushing and grinding the secondary copper sulphide ore of the polymetallic ore in sequence to obtain an ore leaching sample; s2, the ore leaching sample is added into a 9K basic salt solution, and ore pulp is obtained; fe < 2 + > is added into the ore pulp, and strains are inoculated; the strain comprises domesticated thiobacillus ferrooxidans; and S3, after the strains are inoculated, Span 80 is added for leaching. According to the method, the Span 80 is added into the leaching system of the secondary copper sulphide ore of the polymetallic ore, the copper leaching rate is remarkably increased, the leaching period is shortened, and the method has the advantages of being easy and convenient to operate, low in cost, low in energy consumption, environmentally friendly and the like.

The invention provides an active electrode material based on compounding of thiobacillus ferrooxidans and alkali-activated cement and a preparation method of the active electrode material. The thiobacillus ferrooxidans microcapsule, the conductive reinforcing material, the ferrite and the alkali-activated cement-based material are compounded, the synergistic effect of iron oxide generated by microbial metabolism and the conductive material is utilized, and the redox effect of microorganisms is continuously exerted in the working process of the electrode, so that the conductivity and the electrochemical performance of the electrode are remarkably improved; through the local corrosion effect of microbial metabolism acidic products, a through hierarchical pore structure is formed, and the specific surface area is increased. The electrode material has the advantages of low cost, environmental protection, high performance and self-repairing function, and is suitable for the field of new energy storage.

The invention discloses a method for bioleaching a retired ternary lithium battery positive electrode material under combined catalysis of an extracellular polymer and Cu < 2 + >, which comprises the following steps: (1) discharging, roasting, disassembling and grinding a retired ternary lithium battery to obtain ternary lithium battery positive electrode material powder; (2) carrying out mixed culture on the Acidithiobacillus ferrooxidans and the Acidithiobacillus thiooxidans, so as to obtain a mixed bacterial solution; (3) extracting an extracellular polymeric substance from the mixed bacterial liquid obtained in the step (2), and adding the extracellular polymeric substance and Cu < 2 + > into the mixed bacterial liquid obtained in the step (2) to obtain a biological leaching agent; and (4) the waste ternary lithium battery powder obtained in the step (1) is added into the biological leaching agent prepared in the step (3) for a biological leaching reaction for 3-7 days, and a biological leaching solution is obtained. According to the method, the bioleaching rate of metal is increased through the extracellular polymeric substance and Cu < 2 + >, operation is easy and convenient to implement, and the catalyst is simple and easy to obtain.

The invention provides an improved material based on microorganism and mineral synergy and a preparation method and application thereof, the application comprises a method for improving saline-alkali soil by using the improved material, and the method comprises the following steps: carrying out water bath shaking culture on a thiobacillus ferrooxidans solution to obtain a thiobacillus ferrooxidans microbial inoculum; the method comprises the following steps: removing impurities from high-sulfur coal gangue, air-drying, crushing and grinding to obtain coal gangue powder; mixing the coal gangue powder with water, inoculating the mixture with a thiobacillus ferrooxidans microbial inoculum for culturing, and introducing fungi to obtain biologically activated coal gangue; determining the application mass ratio and the total application amount of the bioactive coal gangue and the low-sulfur coal gangue; mixing the bioactive coal gangue with low-sulfur coal gangue to obtain an improved material; pretreating the soil to obtain a saline-alkali soil sample; performing mixed culture on the improved material and the saline-alkali soil sample, and verifying effectiveness; and after verification, applying the improvement material to the target saline-alkali soil. According to the method, the high-sulfur coal gangue is activated by using a microbial technology, the saline-alkali soil improvement efficiency is improved, and waste is turned into wealth.

The present invention discloses a cement slurry for cementing carbon dioxide injection and production wells, its preparation method and application. By weight, the cement slurry for cementing comprises the following components: 95 - 105 parts of oil well cement, 44 - 48 parts of water, 4 - 6 parts of polymer emulsion, 4 - 6 parts of self-healing material, 1.5 - 2.5 parts of drag reducer, 0.5 - 1.5 parts of fluid loss reducer and 0.2 - 0.8 parts of defoamer; wherein, the polymer emulsion is an emulsion polymerized from methyl methacrylate, vinyltrimethoxysilane and propylene carbonate as monomers; the self-healing material is a microcapsule embedding Thiobacillus ferrooxidans cells and Bacillus amyloliquefaciens cells. The cement slurry for cementing maintains good strength and low permeability in a carbon dioxide environment, has good corrosion resistance and has self-healing ability.

The invention discloses a method for efficiently leaching nickel in waste power battery positive electrode material wet treatment tailings, which comprises the following steps: collecting the tailings generated by waste power battery positive electrode material wet treatment, and pretreating the tailings to obtain nickel slag; and the mixed bacterial liquid is put into an improved culture medium to form a leaching system, the pH value of the leaching system is adjusted to 1.8-2.2, the temperature of the leaching system is adjusted to 40-45 DEG C, then the nickel slag is added for leaching treatment, and a nickel-rich solution and leaching slag with the nickel content smaller than or equal to 0.1% are obtained through leaching. According to the method, the nickel slag is efficiently leached by using the mixed bacterial liquid compounded by thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans, thiobacillus caldus and temperature-resistant thiobacillus oxydans, nickel can be fully recycled, and the problems of waste of nickel resources and potential environmental pollution of nickel slag treatment are avoided.

This invention discloses a method and its application for regulating the oxidation and arsenic speciation transformation of arsenopyrite. The method involves sequentially adding arsenopyrite and pyrrhotite to an acidic, iron-free medium with an initial pH of 2.0 to form a protective galvanic cell coupling, followed by inoculation with *Thiobacillus ferrooxidans* and a shaking reaction. By adjusting the ratio of arsenopyrite to pyrrhotite and the addition of *Thiobacillus ferrooxidans*, the method achieves precise regulation of the oxidation of arsenopyrite and the arsenic speciation transformation through phased changes in arsenic release behavior and the As(III) / As(V) ratio. This method possesses dual application value in resource utilization and environmental remediation, and can be applied to the optimization of pretreatment processes for the biological oxidation of gold-bearing arsenic ores or the control of arsenic pollution migration and transformation in acidic mine wastewater, tailings heap leaching, and arsenic-containing sulfide ore coexisting scenarios.

The invention discloses a method for strengthening biological leaching of valuable metals in lead-zinc smelting slag through cooperation of a sulfur source and pH control. The method comprises the following steps: (1) uniformly mixing lead-zinc smelting slag, a sulfur source and a culture medium to obtain slurry; (2) inoculating mixed bacteria consisting of acidophilic thiobacillus thiooxidans and acidophilic thiobacillus ferrooxidans into the slurry; and (3) reacting for a certain time under the condition of keeping the pH value of the slurry basically constant at 1.5-3. Compared with the prior art, the method has the advantages that the residual valuable metal in the lead-zinc smelting slag can be leached out in a green and efficient mode under the mild condition, the original environmental burden is converted into valuable resources, and the concept of sustainable development and circular economy is met.

The invention discloses a method for recycling copper and zinc from high-iron low-grade copper-zinc ore through biological leaching and application, and relates to the technical field of comprehensive utilization of mineral resources. The method for recycling copper and zinc through biological leaching of the high-iron low-grade copper-zinc ore comprises the steps that a mixed flora containing ferrous oxide thiobacillus, thiobacillus thiooxidans, leptospirillum ferriphilum and acidithiobacillus caldus is domesticated, and ore leaching bacterial liquid is obtained; the method comprises the following steps: carrying out biological leaching treatment on a mixture of high-iron low-grade copper-zinc ores by adopting an ore leaching bacterial solution, reducing Fe < 3 + > in a leaching solution into Fe < 2 + >, removing iron in a reducing solution by combining a potential-controlled goethite method, and finally, sequentially recovering copper and zinc. The method can realize efficient recovery of copper and zinc, and has the advantages of environmental protection and low cost.

The application discloses a sulfur-based microbial compound fertilizer with a temperature response type slow-release function and a preparation method thereof, and belongs to the field of microbial compound fertilizers.The compound fertilizer is sequentially composed of a compound organic carrier core, a temperature response type supramolecular gel layer, a pH response controlled release coating layer and a double-layer microcapsule microbial agent layer.The carrier core is made of mixed rotten straw and white liquor lees, and adsorbs elemental sulfur and phosphorus and potassium nutrients.The gel layer is wrapped by beta-cyclodextrin to nitrogen and sulfur nutrients.The coating layer contains thiourea pore-forming agents and chitosan pH response components.The microbial agent layer embeds mixed microbial agents of bacillus subtilis and sulfur-oxidizing bacteria.The application constructs a triple synergistic controlled release mechanism and a three-level sulfur supply system, realizes temperature synchronous release of the microbial agents and nutrients, and matches the nutrient release with crop demand with a matching degree of greater than or equal to 92%.The total viable bacteria survival rate is greater than or equal to 92%.The application is fully biodegradable and has no environmental residues.Field test results show that the application can increase the yield of winter wheat by 29.9% to 34.6%.The raw materials are agricultural wastes, and the production cost is controllable.

The invention relates to the technical field of bioleaching, in particular to a microbial agent for extracting heavy metals in incineration fly ash, which comprises a compound bacteria solution, an activating agent and a carrier in a liquid-solid ratio of 1ml: 0.4 g: (4-5g), the compound bacteria liquid comprises thiobacillus ferrooxidans, bacillus and leptospirillum ferriphilum in a volume ratio of 1: 1: (0.8-1.2); the carrier comprises an algae-based compound, rhamnolipid and sodium alginate in a mass ratio of (3-5): 1: 1; the application method of the microbial agent comprises the following steps: S1, fly ash pretreatment; s2, heavy metal leaching; s3, merging and extracting; sulfuric acid and ferric sulfate are generated through sulfur oxide and ferrous ions, an acid environment is created, heavy metal is directly dissolved, the contact area is increased by dispersing fly ash particles through bacillus and rhamnolipid, and the leaching rate of Cr, Cu and Zn is increased; the carrier and the activating agent provide attachment points for microorganisms and can buffer the impact of high-concentration heavy metal ions in the fly ash on the microorganisms.

The invention relates to the technical field of biological treatment of solid wastes, and discloses thiobacillus thiooxidans resistant to toxicity of Ni and Cu and application of the thiobacillus thiooxidans. Wherein the thiobacillus thiooxidans is the thiobacillus thiooxidans QH2C, and the preservation number of the thiobacillus thiooxidans is GDMCC No: 63521. The invention further discloses a preparation method of the thiobacillus thiooxidans. The invention provides a novel thiobacillus thiooxidans QH2C, and the strain has high toxicity tolerance to Ni and Cu, so that the strain can be used for treating metal-containing solid wastes with higher ore pulp density in batches, the biological leaching treatment efficiency is improved, and the application prospect is good.

The invention provides a coal desulfurization microbial complex microbial inoculant based on gene modification and application thereof, and belongs to the technical field of microorganisms. The preservation number of the pseudomonas putida-coal-09 is CGMCC (China General Microbiological Culture Collection Center) No. 35656. The microbial complex microbial inoculant comprises pseudomonas putida (Pseudomonas putida)-coal-09 and thiobacillus ferrooxidans (Thiobacillus ferrooxidans). Experiments show that when the pseudomonas putida-coal-09 is used for treating a fine coal sample, the desulfurization rate reaches 79.49%, when the pseudomonas putida-coal-09 is used for treating coarse coal, the desulfurization rate reaches 66.49%, and the desulfurization rate of the microbial compound inoculant on the fine coal sample is 84.55%. In conclusion, the pseudomonas putida-coal-09 and the microbial complex microbial inoculant provided by the invention can efficiently remove sulfur in coal, and a foundation is laid for application of a microbial desulfurization technology.

This invention discloses a method for rapidly and stably leaching rare earth elements from phosphate rock using *Thiobacillus ferrooxidans*. The specific steps are as follows: (1) Activate and culture *Thiobacillus ferrooxidans* to obtain a bacterial suspension; (2) Use the activated bacterial suspension to leach rare earth elements from phosphate rock; (3) Conduct leaching experiments under different rotation speeds, aeration rates, and leaching methods, and select the optimal parameters for rapid and stable leaching of rare earth elements. The *Thiobacillus ferrooxidans* provided by this invention has acidophilic and aerobic properties and is widely present in acidic mine wastewater. Its own metabolic activities and metabolic products are conducive to the leaching of rare earth elements from phosphate rock, and it has the advantages of good leaching effect, low cost, and green environmental protection.

The invention discloses application of a polyacrylamide retarder, an alkali-activated cementing material and a preparation method of the alkali-activated cementing material, and belongs to the field of cementing materials. According to the application, polyacrylamide is used as a retarder in an alkali-activated cementing material and is used for regulating and controlling the setting time. The material comprises a cementing material, a composite alkali activator Na2O.nSiO2 and a retarder polyacrylamide, wherein the cementing material comprises 30 wt%-40 wt% of modified printing and dyeing sludge, 40 wt%-55 wt% of slag and 10 wt%-25 wt% of fly ash; the water cement ratio is 0.35 to 0.6. In the preparation process of the modified printing and dyeing sludge, anaerobic-aerobic pretreatment (pH = 5.0, 35 DEG C, and 5% of thiobacillus ferrooxidans) is adopted, so that the azo dye degradation rate is greater than 90% (UV-Vis detection 550nm characteristic peak disappears), heavy metals (such as Cr < 3 + >) are converted into a stable sulfuration state, the limitation that heavy metal migration cannot be solved by traditional acid modification is broken through, and the method is suitable for industrial production. And the cementing material meets the GB 30760-2014 Technical Specification for Co-Treatment of Solid Waste in Cement Cement (leached Cr is less than 0.1 mg / L), and environmental protection obstacles are removed for high-dosage application.

The invention relates to the technical field of cobalt recovery, in particular to a method for efficiently leaching cobalt in battery positive electrode material tailings treated by a wet process, which comprises the following steps: S1, separately culturing thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans and temperature-resistant thiobacillus oxydans; s2, performing compound culture on the separately cultured thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans and temperature-resistant thiobacillus oxydans to obtain a mixed strain solution; s3, the mixed strain liquid is put into an improved culture medium to serve as a leaching system, the cobalt residues obtained through wet treatment are added into the leaching system to be stirred and leached, and a cobalt-rich solution and leaching residues are obtained; the method provided by the invention realizes green, low-carbon and economic recovery of residual metal elements in the wet-process treated tailings, and is short in process, low in investment, high in efficiency, low in treatment cost and suitable for various waste residues containing heavy metals.

The invention relates to the technical field of feed preparation, in particular to a feed for replacing corn and a preparation method thereof.The preparation method comprises the following preparation steps that S1, pretreated pennisetum sinese is put into a mixed solution of water, ethyl alcohol and acetic acid, and primary filtrate and primary filter residues are obtained; s2, stirring and mixing the primary filtrate obtained in the step S1 and the pretreated edible fungus residues to obtain secondary filtrate and secondary filter residues; s3, mixing the secondary filter residues with water to obtain a mixture, adding thiobacillus ferrooxidans and thiobacillus thiooxidans into the mixture for fermentation, and performing centrifugal filtration to obtain tertiary filter residues; and S4, mixing the third filter residue with the first filter residue and the second filter residue, and adding laccase, lactic acid bacteria, lactobacillus plantarum and saccharomycetes for fermentation to obtain a feed finished product. The feed is prepared through combined fermentation of the edible fungus residues and the pennisetum sinese, corn feed can be effectively replaced, and meanwhile the content of heavy metal in the feed is low.

This application provides a composite microbial community and a method for copper extraction by bioleaching assisted by a coupled electric field. The composite microbial community includes *Thiobacillus ferrooxidans*, *Aspergillus niger*, and *Gnaphalium affine*. The composite microbial community is obtained by inoculating *Thiobacillus ferrooxidans*, *Aspergillus niger*, and *Gnaphalium affine* in a composite culture medium at an inoculation ratio of 2:(0.5~2):(0.5~2) and co-culturing them. Under the assistance of an electric field, the composite microbial community synergistically promotes the leaching and extraction of copper from copper-containing substances. This method, through the coupling and coordination of biological and electric field effects, improves the copper leaching and recovery efficiency, and has the advantages of mild conditions, environmental friendliness, and high resource utilization efficiency.

The invention relates to the crossing field of a microorganism utilization technology and an environmental protection technology, and provides a method for immobilizing thiobacillus ferrooxidans by using basalt fiber as a carrier and the basalt fiber carrier. The method comprises the following steps: 1, mixing a thiobacillus ferrooxidans active bacterium solution with a 9k liquid culture medium to prepare an immobilized culture solution; 2, carrying out acid etching modification on the basalt fiber carrier; and 3, filling the prepared immobilized culture solution and the basalt fiber carrier subjected to acid etching modification into an immobilization reactor, and circulating the immobilized culture solution to immobilize the thiobacillus ferrooxidans. The application limitation caused by slow growth of the thiobacillus ferrooxidans under conventional conditions is overcome, and the biomass and the biological oxidation efficiency of the thiobacillus ferrooxidans are greatly improved.