19 results about "Thiobacillus ferrooxidans" patented technology

The invention discloses a method for synergistically enhancing biological oxidation of arsenopyrite by utilizing symbiotic minerals, which particularly comprises the following steps: adding a certain amount of pyrite into an arsenopyrite biological oxidation system, and improving the quantity and activity of acidophilic thiobacillus ferrooxidans by utilizing chemical change of a solution generated by oxidation of the pyrite. The mineral leaching capability of the functional microorganisms is optimized; meanwhile, under the dual action of pyrite and microorganisms, the content of Fe < 3 + > in the solution is remarkably increased, and the Fe < 3 + > serves as a main oxidizing agent for oxidizing arsenopyrite; due to the difference between the surface potentials of the pyrite and the arsenopyrite, a galvanic couple effect is formed between minerals, the electron transfer efficiency between the arsenopyrite and the oxidizing agent is accelerated, and finally the purpose of improving the leaching rate of the arsenopyrite in a biological system is achieved; according to the method, the leaching rate of arsenic in an arsenopyrite biological oxidation system is increased by nearly 8 times at most, the reinforcing effect on arsenopyrite oxidation is remarkable, and meanwhile the method is simple in process, mild in condition, easy and convenient to operate and low in cost.

The invention belongs to the technical field of heavy metal contaminated soil bioremediation, and particularly relates to a method for remediating heavy metal contaminated soil through a Fe3C-N-S composite modified electrode enhanced bioelectrochemical system. Graphene oxide, an iron source, a nitrogen source and a sulfur source serve as raw materials, a Fe3C-N-S composite material is prepared through high-temperature calcination, then the Fe3C-N-S composite material is compounded with sodium alginate (SA) hydrogel, the surface of a graphite rod is coated with the composite material, a Fe3C-N-S composite modified electrode is constructed through a CaCl2 cross-linking and freeze-drying technology, then the modified electrode is coupled with a graphite cathode, and the composite material is obtained. And the acidophilic thiobacillus ferrooxidans is combined to construct a bioelectrochemical system, so that the synergistic and efficient leaching of the heavy metals in the soil is realized. The conductivity, biocompatibility and corrosion resistance of the electrode can be remarkably improved, the problems that mass transfer is difficult and microorganism loading is unstable in BES remediation are solved, and a breakthrough technical scheme is provided for green and efficient remediation of heavy metal contaminated soil.

The invention relates to the technical field of solid waste biological treatment, and discloses La and Ce toxicity resistant thiobacillus thiooxidans and application thereof, and the collection number of the thiobacillus thiooxidans is GDMCC No: 63522. The invention provides a novel thiobacillus thiooxidans, and the preservation number of the novel thiobacillus thiooxidans is GDMCC No: 63522. The strain can highly tolerate La and Ce, and can realize biological leaching of solid wastes rich in La or (and) Ce under high ore pulp density, so that more solid wastes can be treated in a single batch, the biological leaching rate is improved, and the strain has a good application prospect.

The invention relates to a metal leaching agent and a method for leaching and recovering metal from a retired photovoltaic module step by step. The metal leaching agent comprises an acidophilic thiobacillus ferrooxidans solution, a hidden acidophilic bacteria solution and a thiobacillus thiooxidans solution, the volume ratio of the acidophilic thiobacillus ferrooxidans liquid to the hidden acidophilic bacteria liquid to the thiobacillus thiooxidans liquid is (0.5-1.5): (0.5-1.5): (0.5-1.5); wherein the concentration of the acidophilic thiobacillus ferrooxidans, the acidophilic hidden bacteria and the thiobacillus thiooxidans is (1-9) * 10 < 8 > cells / mL. Furthermore, the invention provides a method for leaching and recovering metal from the retired photovoltaic module step by step. According to the method, the metal leaching agent is utilized, mild and green recovery of aluminum and silver in the decommissioned photovoltaic cell piece and full-process recovery of the decommissioned photovoltaic module can be achieved through step-by-step biological leaching, and the method has the excellent leaching effect on aluminum and silver.

The invention relates to a water regulating microbial preparation for breeding largemouth bass seedlings in a land-based round pond and application, and belongs to the technical field of aquaculture. The preparation aims to solve the problem of combined pollution caused by synchronous exceeding of ammonia nitrogen, nitrite and hydrogen sulfide in a water body due to accumulation of residual feed and excrement in a land-based round pool high-density breeding environment. According to the scheme, the microbial agent is prepared by physically mixing bacillus standard bacterial powder, photosynthetic bacteria standard bacterial powder, compound nitrifying bacteria standard bacterial powder and thiobacillus standard bacterial powder according to specific parts by weight, wherein the bacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis, and the thiobacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis. The composite nitrifying bacteria standard bacterial powder is formed by mixing nitrite bacteria and nitrifying bacteria, and the thiobacillus thiooxidans standard bacterial powder is formed by mixing thiobacillus thiooxidans and thiobacillus thioparus. The preparation is used for synchronously degrading ammonia nitrogen, nitrite and hydrogen sulfide in aquaculture water, so that normal development of gonads of largemouth bass parents is guaranteed, and the hatching rate of fertilized eggs and the survival rate of early-stage seedlings are increased.

The invention relates to the technical field of hydrometallurgy, in particular to a method for enhancing microbial leaching of secondary copper sulphide ore of polymetallic ore by using a surfactant. The method for enhancing microbial leaching of the secondary copper sulphide ore of the polymetallic ore by using the surfactant comprises the following steps: S1, crushing and grinding the secondary copper sulphide ore of the polymetallic ore in sequence to obtain an ore leaching sample; s2, the ore leaching sample is added into a 9K basic salt solution, and ore pulp is obtained; fe < 2 + > is added into the ore pulp, and strains are inoculated; the strain comprises domesticated thiobacillus ferrooxidans; and S3, after the strains are inoculated, Span 80 is added for leaching. According to the method, the Span 80 is added into the leaching system of the secondary copper sulphide ore of the polymetallic ore, the copper leaching rate is remarkably increased, the leaching period is shortened, and the method has the advantages of being easy and convenient to operate, low in cost, low in energy consumption, environmentally friendly and the like.

The invention provides an improved material based on microorganism and mineral synergy and a preparation method and application thereof, the application comprises a method for improving saline-alkali soil by using the improved material, and the method comprises the following steps: carrying out water bath shaking culture on a thiobacillus ferrooxidans solution to obtain a thiobacillus ferrooxidans microbial inoculum; the method comprises the following steps: removing impurities from high-sulfur coal gangue, air-drying, crushing and grinding to obtain coal gangue powder; mixing the coal gangue powder with water, inoculating the mixture with a thiobacillus ferrooxidans microbial inoculum for culturing, and introducing fungi to obtain biologically activated coal gangue; determining the application mass ratio and the total application amount of the bioactive coal gangue and the low-sulfur coal gangue; mixing the bioactive coal gangue with low-sulfur coal gangue to obtain an improved material; pretreating the soil to obtain a saline-alkali soil sample; performing mixed culture on the improved material and the saline-alkali soil sample, and verifying effectiveness; and after verification, applying the improvement material to the target saline-alkali soil. According to the method, the high-sulfur coal gangue is activated by using a microbial technology, the saline-alkali soil improvement efficiency is improved, and waste is turned into wealth.

The invention discloses a method for efficiently leaching nickel in waste power battery positive electrode material wet treatment tailings, which comprises the following steps: collecting the tailings generated by waste power battery positive electrode material wet treatment, and pretreating the tailings to obtain nickel slag; and the mixed bacterial liquid is put into an improved culture medium to form a leaching system, the pH value of the leaching system is adjusted to 1.8-2.2, the temperature of the leaching system is adjusted to 40-45 DEG C, then the nickel slag is added for leaching treatment, and a nickel-rich solution and leaching slag with the nickel content smaller than or equal to 0.1% are obtained through leaching. According to the method, the nickel slag is efficiently leached by using the mixed bacterial liquid compounded by thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans, thiobacillus caldus and temperature-resistant thiobacillus oxydans, nickel can be fully recycled, and the problems of waste of nickel resources and potential environmental pollution of nickel slag treatment are avoided.

This invention discloses a method and its application for regulating the oxidation and arsenic speciation transformation of arsenopyrite. The method involves sequentially adding arsenopyrite and pyrrhotite to an acidic, iron-free medium with an initial pH of 2.0 to form a protective galvanic cell coupling, followed by inoculation with *Thiobacillus ferrooxidans* and a shaking reaction. By adjusting the ratio of arsenopyrite to pyrrhotite and the addition of *Thiobacillus ferrooxidans*, the method achieves precise regulation of the oxidation of arsenopyrite and the arsenic speciation transformation through phased changes in arsenic release behavior and the As(III) / As(V) ratio. This method possesses dual application value in resource utilization and environmental remediation, and can be applied to the optimization of pretreatment processes for the biological oxidation of gold-bearing arsenic ores or the control of arsenic pollution migration and transformation in acidic mine wastewater, tailings heap leaching, and arsenic-containing sulfide ore coexisting scenarios.

The invention discloses a method for strengthening biological leaching of valuable metals in lead-zinc smelting slag through cooperation of a sulfur source and pH control. The method comprises the following steps: (1) uniformly mixing lead-zinc smelting slag, a sulfur source and a culture medium to obtain slurry; (2) inoculating mixed bacteria consisting of acidophilic thiobacillus thiooxidans and acidophilic thiobacillus ferrooxidans into the slurry; and (3) reacting for a certain time under the condition of keeping the pH value of the slurry basically constant at 1.5-3. Compared with the prior art, the method has the advantages that the residual valuable metal in the lead-zinc smelting slag can be leached out in a green and efficient mode under the mild condition, the original environmental burden is converted into valuable resources, and the concept of sustainable development and circular economy is met.

The invention discloses a method for recycling copper and zinc from high-iron low-grade copper-zinc ore through biological leaching and application, and relates to the technical field of comprehensive utilization of mineral resources. The method for recycling copper and zinc through biological leaching of the high-iron low-grade copper-zinc ore comprises the steps that a mixed flora containing ferrous oxide thiobacillus, thiobacillus thiooxidans, leptospirillum ferriphilum and acidithiobacillus caldus is domesticated, and ore leaching bacterial liquid is obtained; the method comprises the following steps: carrying out biological leaching treatment on a mixture of high-iron low-grade copper-zinc ores by adopting an ore leaching bacterial solution, reducing Fe < 3 + > in a leaching solution into Fe < 2 + >, removing iron in a reducing solution by combining a potential-controlled goethite method, and finally, sequentially recovering copper and zinc. The method can realize efficient recovery of copper and zinc, and has the advantages of environmental protection and low cost.

The application discloses a sulfur-based microbial compound fertilizer with a temperature response type slow-release function and a preparation method thereof, and belongs to the field of microbial compound fertilizers.The compound fertilizer is sequentially composed of a compound organic carrier core, a temperature response type supramolecular gel layer, a pH response controlled release coating layer and a double-layer microcapsule microbial agent layer.The carrier core is made of mixed rotten straw and white liquor lees, and adsorbs elemental sulfur and phosphorus and potassium nutrients.The gel layer is wrapped by beta-cyclodextrin to nitrogen and sulfur nutrients.The coating layer contains thiourea pore-forming agents and chitosan pH response components.The microbial agent layer embeds mixed microbial agents of bacillus subtilis and sulfur-oxidizing bacteria.The application constructs a triple synergistic controlled release mechanism and a three-level sulfur supply system, realizes temperature synchronous release of the microbial agents and nutrients, and matches the nutrient release with crop demand with a matching degree of greater than or equal to 92%.The total viable bacteria survival rate is greater than or equal to 92%.The application is fully biodegradable and has no environmental residues.Field test results show that the application can increase the yield of winter wheat by 29.9% to 34.6%.The raw materials are agricultural wastes, and the production cost is controllable.

The invention relates to the technical field of bioleaching, in particular to a microbial agent for extracting heavy metals in incineration fly ash, which comprises a compound bacteria solution, an activating agent and a carrier in a liquid-solid ratio of 1ml: 0.4 g: (4-5g), the compound bacteria liquid comprises thiobacillus ferrooxidans, bacillus and leptospirillum ferriphilum in a volume ratio of 1: 1: (0.8-1.2); the carrier comprises an algae-based compound, rhamnolipid and sodium alginate in a mass ratio of (3-5): 1: 1; the application method of the microbial agent comprises the following steps: S1, fly ash pretreatment; s2, heavy metal leaching; s3, merging and extracting; sulfuric acid and ferric sulfate are generated through sulfur oxide and ferrous ions, an acid environment is created, heavy metal is directly dissolved, the contact area is increased by dispersing fly ash particles through bacillus and rhamnolipid, and the leaching rate of Cr, Cu and Zn is increased; the carrier and the activating agent provide attachment points for microorganisms and can buffer the impact of high-concentration heavy metal ions in the fly ash on the microorganisms.

The invention relates to the technical field of biological treatment of solid wastes, and discloses thiobacillus thiooxidans resistant to toxicity of Ni and Cu and application of the thiobacillus thiooxidans. Wherein the thiobacillus thiooxidans is the thiobacillus thiooxidans QH2C, and the preservation number of the thiobacillus thiooxidans is GDMCC No: 63521. The invention further discloses a preparation method of the thiobacillus thiooxidans. The invention provides a novel thiobacillus thiooxidans QH2C, and the strain has high toxicity tolerance to Ni and Cu, so that the strain can be used for treating metal-containing solid wastes with higher ore pulp density in batches, the biological leaching treatment efficiency is improved, and the application prospect is good.

The invention provides a coal desulfurization microbial complex microbial inoculant based on gene modification and application thereof, and belongs to the technical field of microorganisms. The preservation number of the pseudomonas putida-coal-09 is CGMCC (China General Microbiological Culture Collection Center) No. 35656. The microbial complex microbial inoculant comprises pseudomonas putida (Pseudomonas putida)-coal-09 and thiobacillus ferrooxidans (Thiobacillus ferrooxidans). Experiments show that when the pseudomonas putida-coal-09 is used for treating a fine coal sample, the desulfurization rate reaches 79.49%, when the pseudomonas putida-coal-09 is used for treating coarse coal, the desulfurization rate reaches 66.49%, and the desulfurization rate of the microbial compound inoculant on the fine coal sample is 84.55%. In conclusion, the pseudomonas putida-coal-09 and the microbial complex microbial inoculant provided by the invention can efficiently remove sulfur in coal, and a foundation is laid for application of a microbial desulfurization technology.

The invention discloses application of a polyacrylamide retarder, an alkali-activated cementing material and a preparation method of the alkali-activated cementing material, and belongs to the field of cementing materials. According to the application, polyacrylamide is used as a retarder in an alkali-activated cementing material and is used for regulating and controlling the setting time. The material comprises a cementing material, a composite alkali activator Na2O.nSiO2 and a retarder polyacrylamide, wherein the cementing material comprises 30 wt%-40 wt% of modified printing and dyeing sludge, 40 wt%-55 wt% of slag and 10 wt%-25 wt% of fly ash; the water cement ratio is 0.35 to 0.6. In the preparation process of the modified printing and dyeing sludge, anaerobic-aerobic pretreatment (pH = 5.0, 35 DEG C, and 5% of thiobacillus ferrooxidans) is adopted, so that the azo dye degradation rate is greater than 90% (UV-Vis detection 550nm characteristic peak disappears), heavy metals (such as Cr < 3 + >) are converted into a stable sulfuration state, the limitation that heavy metal migration cannot be solved by traditional acid modification is broken through, and the method is suitable for industrial production. And the cementing material meets the GB 30760-2014 Technical Specification for Co-Treatment of Solid Waste in Cement Cement (leached Cr is less than 0.1 mg / L), and environmental protection obstacles are removed for high-dosage application.

The invention relates to the technical field of cobalt recovery, in particular to a method for efficiently leaching cobalt in battery positive electrode material tailings treated by a wet process, which comprises the following steps: S1, separately culturing thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans and temperature-resistant thiobacillus oxydans; s2, performing compound culture on the separately cultured thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans and temperature-resistant thiobacillus oxydans to obtain a mixed strain solution; s3, the mixed strain liquid is put into an improved culture medium to serve as a leaching system, the cobalt residues obtained through wet treatment are added into the leaching system to be stirred and leached, and a cobalt-rich solution and leaching residues are obtained; the method provided by the invention realizes green, low-carbon and economic recovery of residual metal elements in the wet-process treated tailings, and is short in process, low in investment, high in efficiency, low in treatment cost and suitable for various waste residues containing heavy metals.

This application provides a composite microbial community and a method for copper extraction by bioleaching assisted by a coupled electric field. The composite microbial community includes *Thiobacillus ferrooxidans*, *Aspergillus niger*, and *Gnaphalium affine*. The composite microbial community is obtained by inoculating *Thiobacillus ferrooxidans*, *Aspergillus niger*, and *Gnaphalium affine* in a composite culture medium at an inoculation ratio of 2:(0.5~2):(0.5~2) and co-culturing them. Under the assistance of an electric field, the composite microbial community synergistically promotes the leaching and extraction of copper from copper-containing substances. This method, through the coupling and coordination of biological and electric field effects, improves the copper leaching and recovery efficiency, and has the advantages of mild conditions, environmental friendliness, and high resource utilization efficiency.

The invention relates to the crossing field of a microorganism utilization technology and an environmental protection technology, and provides a method for immobilizing thiobacillus ferrooxidans by using basalt fiber as a carrier and the basalt fiber carrier. The method comprises the following steps: 1, mixing a thiobacillus ferrooxidans active bacterium solution with a 9k liquid culture medium to prepare an immobilized culture solution; 2, carrying out acid etching modification on the basalt fiber carrier; and 3, filling the prepared immobilized culture solution and the basalt fiber carrier subjected to acid etching modification into an immobilization reactor, and circulating the immobilized culture solution to immobilize the thiobacillus ferrooxidans. The application limitation caused by slow growth of the thiobacillus ferrooxidans under conventional conditions is overcome, and the biomass and the biological oxidation efficiency of the thiobacillus ferrooxidans are greatly improved.