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Method for detecting adsorbed-state microorganism in chalcopyrite biological leaching process

A bioleaching and detection method technology, which is applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve the problem of high-efficiency qualitative and quantitative detection methods for adsorbed microorganisms, extreme environment of leaching system, turbidity, etc. problems, to achieve the effect of low equipment requirements, simple and easy operation, and good stability

Active Publication Date: 2013-09-04
JIANGNAN UNIV
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  • Abstract
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Problems solved by technology

However, due to the turbidity of the chalcopyrite bioleaching sample solution and the extreme environment of the leaching system, there is no efficient qualitative and quantitative detection method for the adsorbed microorganisms in the bioleaching process.

Method used

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  • Method for detecting adsorbed-state microorganism in chalcopyrite biological leaching process
  • Method for detecting adsorbed-state microorganism in chalcopyrite biological leaching process
  • Method for detecting adsorbed-state microorganism in chalcopyrite biological leaching process

Examples

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Effect test

Embodiment 1

[0033] Example 1 Determination of the total bacterial concentration in the adsorbed state during the leaching process of different strains of chalcopyrite

[0034] Low-grade chalcopyrite originates from the Tonglingshan mining area in Anhui Province. The copper grade is 1.0%. The other main components are as follows: iron 31.8%, sulfur 12.9%, calcium 3.65%, magnesium 3.77%, aluminum 1.45%, and zinc 0.054%. The ore is preliminarily crushed and screened with a 100-mesh sieve to control the particle size of the ore powder to 7 individual / mL, (NH 4 ) 2 SO 4 3.0 g / L, K 2 HPO 4 0.5 g / L, MgSO 4 ·7H 2 O 0.5 g / L, KCl 0.1 g / L, Ca(NO 3 ) 2 0.01 g / L. Thiobacillus thiooxidans ZJJN-1 pure bacteria system: 100mL Starkey medium, cell concentration 5.0×10 7 individual / mL, (NH 4 ) 2 SO 4 3.0 g / L, KH 2 PO 4 3.5 g / L, MgSO 4 ·7H 2 O 0.5 g / L, CaCl 2 2H 2 O 0.25 g / L. Mixed strains of two bacteria (cell number concentration 1︰1): 50mL 9K medium + 50mL Starkey medium, the total...

Embodiment 2

[0038] Example 2 Qualitative and quantitative detection of adsorbed strains in the mixed strain system of Thiobacillus ferrooxidans CUMT-1 and Thiobacillus thiooxidans ZJJN-1

[0039] In this experiment, the Thiobacillus ferrooxidans probe set, including the main probe, capture probe and signal probe, has been applied for a patent, patent number: 201110169853.4.

[0040] According to the comparison with the close relatives of the strain, there are at least two or more base differences, indicating that the specificity is good. Using a 16S rRNA-based detection process, such as figure 2 .

[0041] (1) Preparation of streptavidin-coated plates: Streptavidin was prepared with coating solution at 5 μg / mL, and 100 μL was added to each well of the microplate, sealed with a parafilm and kept overnight at 4°C for the second time. Take out every day, wash 3 times with PBST washing solution, then use 250 μL of 0.1% BSA (solvent is 0.1mol / L PBS buffer solution with pH 7.2), block at 37°...

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Abstract

The invention discloses a method for detecting an adsorbed-state microorganism in a chalcopyrite biological leaching process, and belongs to the technical field of biological leaching. The method disclosed by the invention comprises a washing method applied to an absorbed strain on the mineral surface, the calculation of total concentration of absorbed-state bacteria, and qualitative and quantitative detection method applied to the microorganism in a mixed bacteria system. Three different leaching systems applied to the chalcopyrite biological leaching, namely a pure thiobacillus ferrooxidans CUMT-1 system, a pure thiobacillus thiooxidans ZJJN-1 system and a mixed thiobacillus ferrooxidans and thiobacillus thiooxidans system (number concentration 1:1), are used as examples for verification, and the verification results prove that the method disclosed by the invention has the advantages of simple and easy operation implementation, low requirements for equipment, good specificity and high stability in the detection of the mixed system, and the like, can fast achieve the qualitative and quantitative detection on the absorbed-state microorganism in the biological leaching process, provides basic technical measures for improving the leaching rate, and is suitable for popularization and application.

Description

technical field [0001] The invention discloses a method for detecting microorganisms in an adsorbed state in a chalcopyrite bioleaching process, belonging to the technical field of bioleaching. Background technique [0002] Bioleaching is an interdisciplinary subject in the fields of biology and metallurgy, also known as biooxidation or biohydrometallurgy, which can effectively use microorganisms to accelerate the dissolution rate of lean tailings to recover and refine some precious non-ferrous metals and rare metals to achieve maximum A metallurgical method that maximizes the use of mineral deposits; compared with traditional smelting methods, bioleaching usually has less infrastructure investment, lower operating costs, less environmental pollution and can effectively treat low-grade minerals, so it is considered a green metallurgical technology . With the current increasingly serious environmental problems and the vigorously advocated "environmentally friendly metallurgy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/06G01N33/68C22B3/18C12R1/01
CPCY02P10/20
Inventor 杨海麟冯守帅辛瑜王武张玲
Owner JIANGNAN UNIV
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