40results about How to "Realize qualitative and quantitative detection" patented technology

The invention discloses a visible and reversible fluorescent probe which comprises a cyanine fluorescent group and a benzothiazole group, and the general formula of the probe is shown in the description. The preparation method of the reversible fluorescent probe comprises the following steps: (1) dropwise adding phosphorus oxychloride into salicylaldehyde and paraformaldehyde for reaction, so as to obtain a product 1; (2) enabling the product 1 and hexamethylene-tetramine to react, so as to obtain a product 2; (3) enabling the product 2 and 2-aminobenzenethiol to react at the room temperature, so as to obtain a product 3; (4) enabling the product 3 and a compound 4 to react, so as to obtain the visible and reversible fluorescent probe. According to the ratiometric fluorescent probe provided by the invention, obvious color variation can be found out under natural light or an ultra-violet lamp, and the qualitative detection of sulfur dioxide gas can be implemented under natural light or a hand-held ultra-violet lamp, so that the operation is simple, high convenience and quickness are achieved, and the effect is remarkable; the fluorescent probe can effectively prevent interference from other impurities in samples, and is excellent in selectivity; in addition, the cumbersome pre-processing process of samples is avoided, so that the detection efficiency is high.

The invention discloses a method for recognizing quantitative chiral amino acid by using a reversible nano porphyrin fluorescence sensor, and belongs to the technical field of nano material preparation and chemical analysis detection. The reversible nano porphyrin fluorescence sensor for specifically recognizing quantitative proline, lysine and serine chirality adopts a CdTe quantum dot as a fluorescence probe, and self-assembled porphyrin prepared from a tetra-(4-pyridyl) zinc porphyrin tetrahydrofuran solution and cetyl trimethyl ammonium bromide (CTAB) as a fluorescence quencher, and with the specific combination of the florescence probe and the fluorescence quencher, an openable / closeable nano porphyrin fluorescence sensor can be prepared. The reversible (opening-closing-opening) nano porphyrin fluorescence sensor can be prepared under the action of the openable / closeable nano porphyrin fluorescence sensor with the chiral proline, lysine and serine. Compared with a conventional chromatographic method for recognizing amino acid chirality by separating, the method disclosed by the invention has multiple advantages.

The invention discloses a method for detecting an adsorbed-state microorganism in a chalcopyrite biological leaching process, and belongs to the technical field of biological leaching. The method disclosed by the invention comprises a washing method applied to an absorbed strain on the mineral surface, the calculation of total concentration of absorbed-state bacteria, and qualitative and quantitative detection method applied to the microorganism in a mixed bacteria system. Three different leaching systems applied to the chalcopyrite biological leaching, namely a pure thiobacillus ferrooxidans CUMT-1 system, a pure thiobacillus thiooxidans ZJJN-1 system and a mixed thiobacillus ferrooxidans and thiobacillus thiooxidans system (number concentration 1:1), are used as examples for verification, and the verification results prove that the method disclosed by the invention has the advantages of simple and easy operation implementation, low requirements for equipment, good specificity and high stability in the detection of the mixed system, and the like, can fast achieve the qualitative and quantitative detection on the absorbed-state microorganism in the biological leaching process, provides basic technical measures for improving the leaching rate, and is suitable for popularization and application.

The invention relates to an active field nuclear magnetic resonance detection device and detection method. The device comprises a pre-polarized control circuit used for controlling a transmitting coilthrough a switching switch for generating the pre-polarized magnetic field larger than the natural earth magnetic field, a release circuit which is connected with the transmitting coil through a switch and is used for quickly shutting off remaining energy of a circuit release transmitting coil, an alternating magnetic field control circuit used for controlling the transmitting coil through the switching switch for generating the alternating magnetic field to excite a target water body, and a main control unit used for emitting a control signal to switch and control the pre-polarized control circuit, the release circuit and the alternating magnetic field control circuit. The device is advantaged in that the pre-polarized field (Bp) and the alternating magnetic field (Bac) generated by thecombined current detect the disaster water around a tunnel, the Bp field is used for increasing the signal of the target water and improving the detection signal-to-noise ratio, and the Bac field is used for detecting a detection area layer by layer and can accurately locate the disaster water and realize non-intrusive detection of disaster water bodies under multi-angle and super-noise environments in the tunnel.

The invention discloses a surface enhancement Raman scattering substrate material, and a preparation method and application thereof. The material is composed of silver nanowires and core-shell structures (Ag@Co-Ni LDH) of hollowed structure cobalt-nickel bimetallic hydroxides (Co-Ni LDH), the surface of the substrate material is modified by 4-aminothiophenol, the capturing of aldehyde molecules can be achieved, and the qualitative and quantitative detection on the aldehyde molecules is achieved. Enhanced RGB imaging is conducted on Raman peak intensity at the position of 1623 cm <-1>, the color range is 0-300, three digits of numerical values of R, G and B at an RGB imaging image are read respectively, and bar code or two-dimensional code coding is conducted by using 9 digits of numbers ofRGB and to-be-detected molecular concentration information.

The invention relates to a preparation method of a quantum dot based prostate specific antigen immuno-chromatographic test strip. The test strip is composed of a sample pad, a bonder release pad, a chromatography membrane, and a water absorbing pad, wherein the sample pad and bonder release pad are prepared from preprocessed polyester fibers, and the chromatography membrane and water absorbing pad are made of a preprocessed cellulose nitrate membrane. A sample is dropwise added on the sample pad. The surface of the bonder release pad is painted with quantum dot marked alpha-prostate cancer antigen specific monoclonal antibody. Beta-prostate cancer antigen specific monoclonal antibody and goat-anti-mouse second antibody are coated in the chromatography membrane to form a T line (detection line) and a C line (quality control line). The water absorbing pad drives the liquid to be detected to flow. Compared to the conventional products and technologies, the provided preparation method has the advantages that the whole preparation process is simple, and the preparation method is suitable for industrial production. According to the fluorescent characteristics of quantum dots, the prostate specific antigen can be detected quantitatively and qualitatively, and the specificity is high. The operation is very convenient during the whole process, and the cost is low, so the provided test strip can be applied to tumor screening of high risk groups in communities. A novel method for detecting tumor is established.

The invention discloses a manufacturing method for an immunobiosensor. The manufacturing method comprises the following process steps: 1) manufacturing a reduced graphene modified electrode; 2) manufacturing a prussian blue / graphene modified electrode; and 3) dispensing a porcine epidemic diarrhea virus monoclonal antibody onto the surface of the prussian blue / graphene modified electrode, drying at the room temperature, soaking into a bovine serum albumin solution with mass concentration of 1-2%, and performing water-bath reaction for 0.5-1 hour, thereby obtaining the immunobiosensor. According to the manufacturing method disclosed by the invention, the porcine epidemic diarrhea virus monoclonal antibody is dispensed to the surface of the prussian blue / graphene modified electrode, and the bovine serum albumin solution is utilized to seal the rest of non-specific active sites on the electrode; meanwhile, important process parameters in the manufacturing method are limited, so that the manufactured immunobiosensor has excellent sensitivity, accuracy and stability. The invention further discloses application of the immunobiosensor manufactured from the manufacturing method to clinical quick detection on porcine epidemic diarrhea in non-laboratory setup.

The invention discloses a uric acid detection method based on silver triangular nanoflakes. The uric acid detection method comprises the following steps that the silver triangular nanoflakes are prepared; a to-be-detected sample is added into the silver triangular nanoflakes, then potassium rhodanide is added, and at this moment, if the absorption peak at 400 nm is weakened and the absorption peakat 575 nm rises again, it is expressed that the to-be-detected sample contains uric acid. The detection method is high in flexibility and selectivity.

The invention discloses a method for rapidly determining sulfur species in a solid-phase mineral by utilizing an infrared-programmed heating oxidation combination method. The method comprises the following steps of: (1) calibrating an infrared detector and establishing a standard curve by taking silver sulfide as a calibration standard sample; (2) establishing a sulfur species qualitative database; (3) detecting sulfur species in a to-be-detected solid-phase mineral sample. The step (3) specifically comprises the following steps that: (a) solid-phase mineral powder is put into a roasted container, the container filled with the solid-phase mineral powder is put into a constant-temperature area of a tubular furnace, and the final temperature and heating rate of the constant-temperature areaof the tubular furnace are set; (b) an infrared carbon and sulfur analyzer is turned on, computer control software is started, technical parameters are input, carrier gas oxygen is opened, sampling isstarted after the baseline of the infrared carbon and sulfur analyzer is stable, meanwhile, the constant-temperature area of the tube furnace starts to be heated, after the software of the infrared carbon and sulfur analyzer automatically records a sulfur dioxide escape curve, a sulfur quality curve is derived, and sulfur species in the solid-phase mineral are analyzed. According to the method, O2 is adopted to oxidize or pyrolyze the sulfur species in the solid-phase mineral, and SO2 formed by oxidation or pyrolysis is monitored on line through the infrared carbon and sulfur analyzer.

The invention relates to a method for quantitative determination of catechins, and belongs to the technical field of nanomaterialpreparation and chemical analysis detection. The method for the quantitative determination of the catechins comprises the steps that a four-(4-pyridyl) zinc porphyrinphotosensitive effect of self-assembled nanocrystallization and ZnCdSe quantum dots are used for preparing and obtaining a reversible nanometer porphyrin fluorescence sensor with a nanometer effect in a buffle sollution system; and then strong acting force between the catechins and the nanometer porphyrin is used for opening up different degrees of specific changes of the reversible composite sensing interface between the nanometer porphyrin and the quantum dots, so that the quantitative detection ofthe catechins is realized. The method for the quantitative determination of the catechins is simple and controllable, has high sensitivity, good selectivity and high specificity of the detection method of the catechins, and can play an important role in the detection of the catechins.

The invention discloses a synthesis method of a C-N-GC nano material and application of the C-N@GC nano material in detection of cysteine. The synthesis method comprises the following steps of: stirring and mixing a methanol solution of 2-methylimidazole and a methanol solution of Zn (NO) 2.6 H2O at room temperature to obtain a precipitate ZIF-8, and centrifuging, washing and drying the precipitate to obtain a white powder product; dispersing the synthesized ZIF-8 in a methanol solution by using ultrasonic waves, stirring for 10 minutes, and pouring the methanol solution of 2-methylimidazole and the methanol solution of Co (NO) 2.6 H2O into the solution; stirring the mixed solution at room temperature to obtain a precipitate ZIF-8@ZIF-67, and centrifuging, washing and drying the precipitate to obtain a purple powder product; and taking and putting a certain amount of ZIF-8@ZIF-67 into a muffle furnace, heating at a heating rate of 2 DEG C per minute in an Ar atmosphere, calcining at a constant temperature of 920 DEG C for 2 hours, and cooling to room temperature to obtain a black solid C-N@GC. The C-N@GC is modified on the surface of a glassy carbon electrode for cysteine detection. The application of the nano modified electrode in cysteine detection is promoted.

The invention discloses resorufin-2, 4-dinitrodiphenyl ether and application of the resorufin- 2, 4-dinitrodiphenyl ether to detection of thiophenol. The structural formula of the resorufin- 2, 4-dinitrodiphenyl ether is shown in the Specification. In a phosphate buffer solution with the pH value of 7.4, the resorufin- 2, 4-dinitrodiphenyl ether is in nucleophilic substitution with the thiophenol to enable a reaction system to have strong absorption at the part of which the wavelength is 580 nm. The experimental result shows that the resorufin-2, 4-dinitrodiphenyl ether is adopted to detect the thiophenol, the detection limit and the relative standard deviation of the thiophenol are 8.91*10<-8>mol / L and 3.7% respectively and qualitative and quantitative detection of the thiophenol is realized. The detection method is simple to operate, high in accuracy and excellent in selectivity, and different sulfhydryl compounds cause no interference to the system for detecting the thiophenol by adopting the resorufin-2, 4-dinitrodiphenyl ether.

The invention provides a method for detecting a novel coronavirus neutralizing antibody by using a BLI technology. The method comprises the following steps: capturing human ACE2 protein with the sameconcentration on the surface of a biosensor, and premixing novel coronavirus spinous process protein RBD with neutralizing antibodies to be detected with different concentrations respectively, respectively contacting each mixed solution with the human ACE2 protein captured on the surface of the biosensor, calculating the inhibition rate according to the change of the relative displacement intensity of the interference spectrum detected by a molecular interaction instrument based on the BLI technology, drawing an inhibition curve, and calculating IC50. The method is simple to operate, quick andefficient, coating, repeated sample adding and plate washing are not needed in the whole detection process, and experimental results can be obtained within 15 min. The detection reaction is carried out in a black pore plate, the detection of novel coronavirus neutralizing antibodies of a large batch of samples can be realized, and different from the traditional qualitative detection, the method has the advantages that the inhibition capability of different new crown neutralizing antibodies can be quickly compared by calculating the IC50 value.

The invention relates to a metal organic framework (MOFs) fluorescent probe for detecting tetracycline antibiotics (TCs) and a preparation method of the metal organic framework (MOFs) fluorescent probe, and also provides a fluorescence integration method and an application program for designing, analyzing and recognizing the overall pollution degree of the tetracycline antibiotics. According to the fluorescent probe, 5-aminoisophthalic acid (H2aip) is used as an organic ligand of a stable three-dimensional framework, transition metal Zn (II) is used as a metal source, and the fluorescent probe with unique response to TCs is synthesized. Meanwhile, based on a fluorescence integration method, the method is not limited to identification of a single antibiotic, the pollution degree of the TCs can be quantified through a TCs Analysis application program, through precise data processing and intelligent analysis, a new method is provided for measuring the overall pollution degree of the TCs, and the method is suitable for portable analysis and detection.

An electrochemical immunosensor for detecting CP4-EPSPS protein in crops comprises a glassy carbon electrode and a recognition element, the surface of the glassy carbon electrode is modified by a composite material, and the composite material is formed by ultrasonic treatment of graphene and amino-rich PAMAM; a gold-labeled antibody is used as a recognition element, a colloidal gold-labeled monoclonal antibody CP4mAb is connected to a composite material on the surface of a glassy carbon electrode in a covalent binding mode, and the electrochemical immunosensor is constructed after 5% BSA sealing. According to the invention, the introduction of gold nanoparticles improves the immobilization amount of an electroactive substance and amplifies a detection signal; The sensor is high in detection specificity and sensitivity for herbicide-resistant protein CP4-EPSPS, simple to operate, quick in reaction, high in sensitivity and good in stability, realizes quantitative detection for trace CP4-EPSPS antigen, and provides a new thought for high-sensitivity quantitative analysis of transgenic crops.

The invention discloses a functional multi-nitrogen heterocyclic ring fluorescent probe, and a preparation method and an application of the functional multi-nitrogen heterocyclic ring fluorescent probe, wherein the general structural formula of the functional multi-nitrogen heterocyclic ring fluorescent probe is shown in the specification, wherein R1, R2, R3 and R4 respectively and independently represent hydrophilic groups. The preparation method of the fluorescent probe comprises the following steps: taking bipyridine dimethylamine as a molecular skeleton, rearranging bipyridine dimethylamine after bipyridine dimethylamine is reacted with carbon disulfide to increase the molecular rigidity, and obtaining the organic probe giving out green fluorescence. The fluorescent probe is high in stability and is not easily photobleached. The fluorescent strength and the color of the probe are changed under the action of hypochlorous acid, so that the probe can be used to quantitatively and qualitatively detect the hypochlorous acid, the limit of detection is as low as 0.43 micrometer, and very high selectivity is acquired.

The invention provides a paper-based device based on a moving valve and molecular imprinting technology, and a manufacturing method and application thereof. The manufacturing method comprises the following steps of pattern design, chip fabrication, electrode construction on a paper base, chip connection, synthesis of a functionalized molecularly imprinted polymer layer, electrochemical polymerization, and elution of template molecules. The device is used for analysis and detection of biomarkers in serum samples. The invention provides a paper-based device based on moving valve and molecular imprinting technology, a manufacturing method and application thereof, and provide a novel device for clinically detecting biomarkers. The device is used for in-situ synthesis of biomarker molecularlyimprinted polymer, forms a clinical application method, creates a new strategy for antibody-free biomarker analysis, realizes the entire process from device fabrication to test result output on the paper-based device, and is economical, portable, high in speed.

The invention discloses a surface enhanced Raman scattering substrate material and a preparation method and application thereof. The substrate material is of a core-shell structure and comprises gold superparticles and a metal organic framework of a hollow structure, and the gold superparticles are arranged in the metal organic framework. A pore exists between the inner surface of the metal organic framework and the outer surfaces of the gold superparticles. The substrate material further comprises a functional modification material, and the functional modification material is combined on the outer surfaces of the gold superparticles through chemical bonds. According to the surface enhanced Raman scattering substrate material and the preparation method and application thereof, rapid gas capture, rapid adsorption, specificity and rapid quantitative detection of lung cancer respiratory markers can be realized.

The invention discloses a preparation method and application of a kit for simultaneously detecting various nafil substances based on a novel multi-quantum dot-protein polymer probe. The multi-quantum dot-protein immune probe is prepared by a one-step method based on a novel solvent-induced effect strategy, and the fluorescent immune probe with a large number of fluorescent quantum dots is prepared by mixing the quantum dots, the BSA and the antibody and utilizing hydrophobic interaction and pi-pi accumulation, so that the loading capacity of the quantum dots in the probe is improved, and the preparation method of the probe is simplified. Sildenafil, tadalafil and derivatives thereof are taken as detection objects, sildenafil and tadalafil antibodies and antigens are combined with the method to prepare a probe and a lateral flow immunochromatography test strip for specifically detecting sildenafil, tadalafil and derivatives thereof, a solid-phase extraction micro-fluidic chip is combined to construct a system for detecting nafil substances, and the system is used for detecting the sildenafil, tadalafil and derivatives thereof. Qualitative and quantitative detection of the 12 nafil drugs is achieved, the specificity is high, the sensitivity is high, the accuracy is good, and the detection result is reliable.

The invention belongs to the field of technologies for detecting and analyzing ozone, and particularly relates to a fluorescence probe for visually detecting ozone and a method for preparing the fluorescence probe. 2-(2-hydroxyphenyl) benzothiazole is used as a fluorophore for the fluorescence probe, 4-bromine-1-butene is used as a recognition group, bright green fluorescence can be emitted by thefluorophore in a solid state, the fluorescence is quenched after the fluorophore and the recognition group are connected with each other, the fluorescence probe capable of emitting weak blue light isgenerated, and the fluorophore capable of emitting the bright green fluorescence can be restored after reaction is carried out on the fluorescence probe and the ozone. The fluorescence probe and themethod have the advantages that the liquid-state and gas-state ozone can be visually, qualitatively and quantitatively detected by the fluorescence probe, and the fluorescence probe is good in selectivity and high in anti-interference performance.

The invention discloses a fluorescent probe containing N, N-diethyl paramethylaminophenol and application thereof in the thiophenol detection. The structural formula of the fluorescent probe is as follows: in a phosphate buffered solution with pH value of 7.4, the compound can be in nucleophilic substitution reaction with thiophenol for releasing out the strong fluorescent material 3-(N, N-diethyl)-6-hydroxy-fluorane, so that the reaction system has a strong fluorescent signal at the wavelength of 543 nm; experimental results show that the fluorescent probe is used to detect the thiophenol; the detection limit of the thiophenol is 6.0*10<-9>mol / L; the relative standard deviation is 2.6%; the qualitative and quantitative detection of the thiophenol can be realized. The detecting method is simple in operation, high in accuracy and sensitivity and good in selectivity; different thiol compounds have no disturbance to the system of the fluorescent probe detecting the thiophenol; the fluorescent probe can be used for detecting the content of the thiophenol in a water sample.

The invention provides a kit, a use method thereof, and a hydrogen peroxide concentration detection method. The kit comprises a gold nano solution, a cysteine solution and a ferrous ion solution. The kit comprises ferrous ions and can rapidly catalyze hydrogen peroxide to oxidize cysteine, experimental results show that the catalytic process only needs several minutes and has remarkable advantages compared with literature records, and due to the fact that the optimal pH range is slightly neutral, the catalytic process is irrelevant to a Fenton reagent, and the kit belongs to a novel gold nano color development system; and according to the kit, corresponding compounds in a sample to be detected are oxidized by oxidase to generate H2O2, the concentration of the compounds in the sample to be detected is further calculated, qualitative and quantitative detection of multiple compounds such as lactic acid, glucose, cholesterol and uric acid can be achieved, and the kit has huge application value.

The invention discloses a rapid detection method for detecting glyphosate based on EPSP synthase and GFP fusion protein, including the cloned glyphosate main target EPSP synthase as the receptor protein of glyphosate; the receptor protein and the green Fluorescent protein was fused to construct EPSPS‑GFP, a new protein containing both glyphosate-binding sites and fluorescent labels. It was prokaryotically expressed and purified in Escherichia coli, and used for the detection of glyphosate in water. The invention realizes the qualitative and quantitative detection of the glyphosate herbicide by constructing the fusion protein and using a fluorescence photometer to detect the change of the fluorescence response value and establishing a standard calibration curve.