Method for recognizing quantitative chiral amino acid by using reversible nano porphyrin fluorescence sensor

A fluorescent sensor and chiral amino acid technology, applied in fluorescence/phosphorescence, material analysis through optical means, instruments, etc., can solve the problems of complex sample preparation, unavoidable derivative reagents, long detection time, etc., and achieve high sensitivity , fast response, simple preparation

Active Publication Date: 2017-05-24
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional methods include chromatography, electrochemical methods, and electron microscopy. These methods have the characteristics of high sensitivity or strong separation ability, but there are also some shortcomings, such as the difficulty of avoiding the impact of derivatization reagents on the chiral recognition of amino acids, complex Sample preparation, long testing time, etc.

Method used

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  • Method for recognizing quantitative chiral amino acid by using reversible nano porphyrin fluorescence sensor
  • Method for recognizing quantitative chiral amino acid by using reversible nano porphyrin fluorescence sensor
  • Method for recognizing quantitative chiral amino acid by using reversible nano porphyrin fluorescence sensor

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Example 1: The recognition and quantitative analysis of the chirality of proline by the reversible nano-porphyrin fluorescent sensor, the schematic diagram of the method is shown in Figure 1, and the steps are as follows:

[0045] (1) Synthesis of CdTe quantum dot fluorescent probe

[0046] Dissolve cadmium dichloride (0.1142g, 12.5mM) and N-acetyl-L-cysteine ​​(0.0979g, 15mM) in 40mL of ultrapure water, stir at room temperature and pressure for 15 minutes, and then oxidize with hydrogen The pH of the solution was adjusted to 8.00 with sodium solution, and then filled with nitrogen and stirred in an ice bath for 20 minutes. Add sodium tellurite (0.0216g, 2.5mM) and stir for 15 minutes; then add sodium borohydride (0.0113g, 7.5mM) and stir for 15 minutes. Finally, the solution was put into a reaction kettle and reacted in an oven at 200° C. for 50 minutes. Cooling to room temperature yielded 2.9×10 -7 mol / LCdTe quantum dot fluorescent probe.

[0047] (2) Synthesis of...

Embodiment 2

[0053] Example 2: The recognition and quantitative analysis of the chirality of lysine by the reversible nano-porphyrin fluorescent sensor, the schematic diagram of the method is shown in Figure 1, and the steps are as follows:

[0054] (1) Synthesis of CdTe quantum dot fluorescent probe

[0055] The CdTe quantum dot fluorescent probe was synthesized by the method of step (1) in Example 1.

[0056] (2) Synthesis of tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution

[0057] The method of step (2) in Example 1 was used to synthesize tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution.

[0058] (3) Preparation of switch nanoporphyrin fluorescent sensor

[0059] The nano-porphyrin fluorescence sensor was prepared by the method of step (3) in Example 1.

[0060] (4) Recognition and quantitative analysis of D- / L-lysine by reversible nanoporphyrin fluorescent sensor

[0061] Add 100μL- / L-lysine aqueous solution to 1.5mL cuvette, 70μL 3.42×10 -6 mol / L tetrakis-(4-pyri...

Embodiment 3

[0062] Example 3: The recognition and quantitative analysis of the chirality of serine by the reversible nano-porphyrin fluorescent sensor, the schematic diagram of the method is shown in Figure 1, and the steps are as follows:

[0063] (1) Synthesis of CdTe quantum dot fluorescent probe

[0064] The CdTe quantum dot fluorescent probe was synthesized by the method of step (1) in Example 1.

[0065] (2) Synthesis of tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution

[0066] The method of step (2) in Example 1 was used to synthesize tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution.

[0067] (3) Preparation of switch nanoporphyrin fluorescent sensor

[0068] The nano-porphyrin fluorescence sensor was prepared by the method of step (3) in Example 1.

[0069] (4) Recognition and quantitative analysis of D- / L-serine by reversible nanoporphyrin fluorescent sensor

[0070] Add 100μL- / L-serine aqueous solution to 1.5mL cuvette, 70μL 3.42×10 -6 mol / L tetrakis-(4-py...

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Abstract

The invention discloses a method for recognizing quantitative chiral amino acid by using a reversible nano porphyrin fluorescence sensor, and belongs to the technical field of nano material preparation and chemical analysis detection. The reversible nano porphyrin fluorescence sensor for specifically recognizing quantitative proline, lysine and serine chirality adopts a CdTe quantum dot as a fluorescence probe, and self-assembled porphyrin prepared from a tetra-(4-pyridyl) zinc porphyrin tetrahydrofuran solution and cetyl trimethyl ammonium bromide (CTAB) as a fluorescence quencher, and with the specific combination of the florescence probe and the fluorescence quencher, an openable/closeable nano porphyrin fluorescence sensor can be prepared. The reversible (opening-closing-opening) nano porphyrin fluorescence sensor can be prepared under the action of the openable/closeable nano porphyrin fluorescence sensor with the chiral proline, lysine and serine. Compared with a conventional chromatographic method for recognizing amino acid chirality by separating, the method disclosed by the invention has multiple advantages.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and chemical analysis and detection, and in particular relates to a controllable preparation of a novel reversible nanoporphyrin fluorescence sensor and a method for highly sensitive detection of chiral amino acids. Background technique [0002] Chirality is an important property of amino acids, sugars and heterocycles, which are the building blocks of living matter. The difference in microscopic spatial configuration between enantiomers of chiral molecules often leads to huge differences in their macroscopic physiological and pharmacological effects in vivo. Therefore, research on chiral recognition between chiral substances and biomacromolecules is of great significance for revealing the mechanism and rules of biomolecules. Proline, lysine and serine play an important regulatory role in the physiological and pathological processes of organisms. Proline can be used in medicine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 付海燕胡鸥杨天鸣郭晓明范尧佘远斌
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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