A kind of detection method of cysteine

A cysteine ​​and detection method technology, applied in the cysteine ​​detection field, can solve the problems of expensive instruments and equipment, long detection time and the like, and achieve the effects of simple and convenient preparation method, rapid detection and high sensitivity

Active Publication Date: 2022-06-28
NANJING TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a synthesis method of C-N@GC nanomaterials and its detection application in cysteine, so as to solve the problems of long detection time and expensive equipment required in the above-mentioned background technology

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  • A kind of detection method of cysteine
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Embodiment 1

[0025] A method for synthesizing C-N@GC nanomaterials, comprising the following steps:

[0026] (1) Synthesis of ZIF-8: Pour the methanol solution of 2-methylimidazole (6.16 g, 150 mL) into Zn(NO) 2 ·6H 2 O in methanol solution (5.95g, 150mL); secondly, the above-mentioned mixed solution was stirred at room temperature for 24h to obtain a milky white liquid; then the milky white liquid was washed 3 times with methanol and collected by centrifuge; finally, under the condition of 60 degrees Celsius Dry in vacuo overnight to give ZIF-8; and 2-methylimidazole with Zn(NO) 2 ·6H 2 The mass ratio of O is 6.16:5.95;

[0027] (2) Synthesis of ZIF-8@ZIF-67: The ZIF-8 (0.5 g) synthesized in step (1) was dispersed in methanol solution (100 mL) with ultrasonic waves, and after stirring for 10 min, 2-methylimidazole in methanol Solution (6.16g, 100mL) and Co(NO) 2 ·6H 2 The methanol solution of O (5.82g, 100mL) was poured into the above solution; secondly, the mixed solution was stirr...

Embodiment 2

[0030] The application of C-N@GC nanomaterials in cysteine ​​detection includes the following steps:

[0031] S1, configuration of modifier solution: take 5 mg of C-N@GC synthesized in Example 1 and transfer it to a mortar, fully grind it into powder, and put the ground C-N@GC into 1 ml of water-ethanol solution ( volume ratio 3:1), so that the ground C-N@GC can be uniformly dispersed in 1 ml of water-ethanol solution (volume ratio 3:1), and 20 μL of naphthol solution (5wt%) was added dropwise to it, A uniform modifier solution was obtained by ultrasonication for 2 h by ultrasonic degradation;

[0032] S2. Modification on the surface of the glassy carbon electrode: take 3 μL of the modifier solution in S1 and drop it evenly on the surface of the polished glassy carbon electrode, and dry it at room temperature to finally obtain a C-N@GC modified glassy carbon electrode;

[0033] S3. The C-N@GC modified glassy carbon electrode obtained in S2 is used for the electrochemical dete...

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Abstract

The invention discloses a method for detecting cysteine. Including the following steps: S1, the configuration of the modifier solution: take the C-N@GC nanomaterial and disperse it in the water-ethanol solution, and drop a naphthol solution with a weight percentage of 5wt% thereinto, and use the sonication method to sonicate for 2 hours, Obtain a uniform modifier solution; S2. Modification on the surface of the glassy carbon electrode: take the modifier solution in S1 and evenly drop it on the surface of the polished glassy carbon electrode, and dry it at room temperature to finally obtain C‑N@GC modified the glassy carbon electrode; S3, the C-N@GC modified glassy carbon electrode obtained in S2 is used for the electrochemical detection of cysteine; the C-N@GC is modified on the surface of the glassy carbon electrode for the semi Cystine detection. The invention realizes the qualitative and quantitative detection of cysteine ​​through the application of the nano-modified electrode, and provides a new idea and a new method for discovering and constructing a novel electrochemical detection probe.

Description

technical field [0001] The invention relates to the technical field of electroanalysis, in particular to a method for detecting cysteine. Background technique [0002] Cysteine ​​(Cys) is a small molecule containing sulfhydryl groups, and because of its chemical instability, cysteine ​​plays a variety of roles in physiological and biological processes. In biological processes, cysteine ​​is a precursor for the synthesis of glutathione, acetyl-CoA, cystine, etc. It can undergo reversible redox reactions under physiological conditions, which are essential for maintaining the tertiary and quaternary structure of proteins. In addition, cysteine ​​can also be used as a substrate to be catalytically metabolized into bioactive molecules such as pyruvic acid, sulfenic acid, and hydrogen sulfide, and further play a variety of roles in living organisms. A lack of cysteine ​​in the body can lead to hair loss, slow growth, liver damage, edema, weakness and skin damage. Elevated cyste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 王志娟蔡永亮陈剑刘霞胡松
Owner NANJING TECH UNIV
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