329 results about "Glassy carbon electrode" patented technology

The invention discloses an all-solid-state calibration-free ion concentration detection method. The method comprises the following steps: constructing a plurality of traditional three-electrode detection systems; in each traditional three-electrode detection system, current and impedance signals are synchronously acquired through a step potential electrochemical spectrum and an electrochemical impedance spectrum, and interface kinetic parameters are analyzed; on the basis of a traditional three-electrode detection system, an original reference electrode is removed, a counter electrode is reserved, all glassy carbon electrodes with gradient membrane volumes are connected in series one by one to obtain a new three-electrode detection system, the new three-electrode detection system is immersed in a to-be-detected solution, a preset potential is applied, and through transient current response in combination with calibrated kinetic parameters, the gradient membrane volumes of the glassy carbon electrodes are detected. And performing inversion to obtain the ion concentration of the to-be-detected solution. According to the method, automatic calibration is realized by analyzing interface process kinetic parameters of different-volume film ion selective electrodes and calculating the slope to generate a built-in standard curve. According to the method, interface process parameters are obtained through transient current, and dependence of a traditional detection system on the stable potential of a reference electrode is eliminated.

The invention discloses a biosensor for detecting Alzheimer's disease markers as well as a construction method and application thereof, and relates to the technical field of biosensors. The construction method comprises the following steps: after a glassy carbon electrode is cleaned, dropwise adding a RuSi NPs (at) CS compound on the surface of the glassy carbon electrode to obtain a RuSi NPs (at) CS / GCE electrode; and immersing the RuSi NPs coated CS / GCE electrode into a PHL: S double-chain solution, and carrying out an incubation reaction to obtain the biosensor. The RuSi NPs (at) CS compound is obtained by carrying out mixed reaction on ruthenium-silicon nanoparticles and chitosan; pHL: S double chains contained in the PHL: S double-chain solution are obtained by carrying out incubation reaction on carboxyl-activated PHL chains and S chains. The biosensor has the advantages of high accuracy, strong sensitivity, good selectivity and the like, and can be applied to accurate detection of the Alzheimer's disease marker A beta oligomer.

The invention provides a preparation method of a plate-shaped glassy carbon electrode, which comprises the following steps: curing a resin raw material into a plate-shaped resin block, carbonizing in an inert atmosphere, and respectively introducing a nitrogen source and a boron source for doping operation to obtain a co-doped plate-shaped resin block precursor; placing the co-doped plate-shaped resin block between two porous graphite plates, and carrying out high-temperature treatment to obtain a glassy carbon matrix; and immersing the glassy carbon substrate into the adhesion layer solution to form an adhesion layer, transferring the adhesion layer into a deposition solution which comprises a bismuth-containing solution and a gold-containing solution, forming bismuth-gold nano-dots on the adhesion layer, and washing and drying the bismuth-gold nano-dots to obtain the plate-shaped glassy carbon electrode. Through directional arrangement of crystal orientations, the tantalum carbide coating achieves higher electrical conductivity and thermal conductivity. And the sensitivity and the service life of the plate-shaped glassy carbon electrode are obviously improved and prolonged.

The invention discloses a dopamine sensor working electrode based on an MOF material and a preparation method thereof, and belongs to the technical field of electrochemical sensing. The working electrode is composed of a pure MOF Cu-HHTP modified glassy carbon electrode and a Nafion film covering the surface of the glassy carbon electrode, and the preparation method of the working electrode comprises the following steps: synthesizing Cu-HHTP (Cu < 2 + > and a hexahydroxy triphenyl ligand are coordinated to form a two-dimensional honeycomb layer) through a solvothermal method, dispensing Cu-HHTP dispersion liquid on the surface of the glassy carbon electrode, and then covering the glassy carbon electrode with the Nafion film to form the combined electrode. The dopamine sensor prepared by the preparation method disclosed by the invention has high sensitivity, high anti-interference capability and long-term stability. The preparation process is simple, complex conductive additives are not needed, and the method is suitable for rapid and accurate detection of dopamine in complex biological samples.

The invention belongs to the technical field of seawater detection, and particularly relates to a detection method for simultaneously detecting seawater-based Pb (II) and Cd (II), which comprises the following steps: step 1, polishing a glassy carbon electrode: polishing the glassy carbon electrode for three times by using aluminum oxide powder according to an 8-shaped track, then carrying out ultrasonic treatment in ultrapure water for 2-4 minutes, removing surface adhesion substances, washing by using ultrapure water, and drying to obtain the glassy carbon electrode; and activating the glassy carbon electrode in a sulfuric acid solution, scanning by using cyclic voltammetry until a stable cyclic voltammetry curve is obtained, washing by using ultrapure water, and drying at room temperature. Step 2, preparing a graphitized carboxylated multi-walled carbon nanotube solution, namely weighing 3.5 mg of graphitized carboxylated multi-walled carbon nanotubes and 0.8 mg of nano zinc oxide, dissolving the graphitized carboxylated multi-walled carbon nanotubes and the nano zinc oxide in 5ml of dimethylformamide, and performing ultrasonic dispersion for more than 3h to obtain the graphitized carboxylated multi-walled carbon nanotube solution; the method has good stability, reproducibility and anti-interference performance, and can realize high-sensitivity detection of Pb (II) and Cd (II) in actual seawater.

The invention provides a lactic acid electrochemical sensor LDHamp; the invention relates to construction of BiNPs (at) MWCNTs / GCE and a serum lactic acid detection method, and belongs to the technical field of biosensors, the method comprises the following steps: taking sodium citrate, Bi (NO3) 35H2O and NaBH4 as raw materials, synthesizing BiNPs through a multi-step reaction, compounding the BiNPs with MWCNTs, carrying out carboxyl activation and loading LDH, and finally modifying on a glassy carbon electrode to obtain the sensor. During detection, the sensor is placed in a PBS system containing 5mM of NAD, a serum sample is added, and the concentration of lactic acid is measured by electrochemical scanning according to current response change. According to the method, the detection linear range is 0-200 [mu] M, the detection limit is as low as 0.03 [mu] M, the detection difference of different batches of sensors is small, the method shows the advantages of high sensitivity and good stability, meanwhile, efficient and accurate detection of lactic acid in serum is achieved, and powerful support is provided for clinical diagnosis and disease research.

The invention relates to a samarium molybdate / reduced graphene oxide electro-catalytic material, a preparation method and application thereof and an electrochemical sensor, and relates to the technical field of electro-catalytic materials and electrochemical sensors, the preparation method comprises the following steps: dispersing GO in water, and stirring to obtain a GO suspension; the preparation method comprises the following steps: separately adding Sm (NO3) 3.6 H2O and Na2MoO4. 2H2O into water for ultrasonic dissolution, and mixing to obtain a first mixture; stirring and mixing the GO suspension and the first mixture to obtain a second mixture, and the like. The samarium molybdate / reduced graphene oxide electro-catalytic material is synthesized by a hydrothermal synthesis method and is used for modifying a glassy carbon electrode so as to be applied to electrochemical detection of FZD for the first time. Compared with a traditional detection method, the detection method has the advantages of being simple, efficient, green, environmentally friendly, sensitive in response and portable, and a new way is provided for detection of carcinogenic residues.

The invention provides an electrochemical sensor for identifying amino acid enantiomers and a preparation method and application thereof, and relates to the technical field of electrochemistry, the sensor comprises a glassy carbon electrode and a xylose-inulin composite coating coated on the glassy carbon electrode. The preparation method comprises the following steps: S1, preparing the xylose-inulin composite material in a self-assembly manner; and S2, dispensing the xylose-inulin composite material obtained in S1 on the surface of a glassy carbon electrode, and airing to obtain the xylose-inulin modified glassy carbon electrode. The glassy carbon electrode modified by the composite material has a large number of active sites, and the modified electrode shows selective recognition on amino acid enantiomers by effectively utilizing the synergistic effect of xylose and inulin. The electrode disclosed by the invention has good stability, specificity and chiral recognition capability on the amino acid enantiomers, and has good application prospects in quantitative analysis of the amino acid enantiomers.

The invention relates to the technical field of organic synthesis, in particular to 1-chloro-8-fluorine-7-isopropyl benzofuro [2, 3-c] pyridine as well as a synthesis method and an application of the 1-chloro-8-fluorine-7-isopropyl benzofuro [2, 3-c] pyridine. According to the invention, 1-chloro-8-fluoro-7-isopropyl benzofuro [2, 3-c] pyridine and nanogold are cooperated to cover the surface of a glassy carbon electrode (GCE), and a novel electrochemical modified electrode AuNPs / 1 / GCE is developed. The modified glassy carbon electrode shows good selectivity, high stability and sensitivity and wide detection range on the detection of the antibiotic furacilin.

The invention relates to the technical field of electrochemical luminescence detection, in particular to an electrochemical luminescence aptamer sensor based on a CoOOH-cDNA / Apt / AgNWs / Tb (at) A-COF / GCE working electrode. The iBF in a sample to be detected is detected by utilizing LC-MS / MS, GC-MS, UHPLC and HPLC-MS, so that the cost is high, the speed is low, and the sensitivity is relatively low. Aiming at the technical problem, the invention provides the CoOOH-cDNA / Apt / AgNWs / Tb-coated A-COF / GCE working electrode, the working electrode takes Tb-coated A-COF as a donor and CoOOH NSs as a receptor, the donor and the receptor are combined and modified on a glassy carbon electrode (GCE) through an aptamer and cDNA, then the CoOOH-cDNA / Apt / AgNWs / Tb-coated A-COF / GCE is taken as the working electrode, and iBF in a sample to be detected is quantitatively detected. According to the invention, through a signal "on-off-on" (on-off-on) mode, the consistency of detection results is significantly improved, the generation of false positive or false negative signals is reduced, and the detection accuracy is improved.

The invention discloses a bimetallic / carbon nanotube compound modified glassy carbon electrode as well as a preparation method and application thereof, and belongs to the technical field of electrochemical analysis. The bimetallic / carbon nanotube compound modified glassy carbon electrode provided by the invention comprises a glassy carbon electrode substrate and a bimetallic / carbon nanotube compound compounded on the glassy carbon electrode substrate, the bimetallic / carbon nanotube compound is prepared from nickel-cobalt bimetallic nanoparticles and a multi-walled carbon nanotube. The prepared bimetallic / carbon nanotube compound modified glassy carbon electrode has excellent electrocatalytic activity, has the advantages of good stability, low detection limit and high sensitivity and selectivity in the process of determining paracetamol, can realize on-site rapid detection of paracetamol, has the advantages of being convenient to carry and simple to operate, and has a wide application prospect. Wide application prospects are realized in medicine safety and clinical medical treatment.

The invention belongs to the field of electrochemical sensors, and relates to an electrochemical sensor for high-sensitivity detection of diuron and a preparation method of the electrochemical sensor. An HNT (halloysite nanotube)-CNC (carbon nanocage)-SWCNT (single-walled carbon nanotube) nanocomposite material prepared from a halloysite nanotube, a carbon nanocage (CNC) and an SWCNT (single-walled carbon nanotube) is modified on the surface of a GCE (glassy carbon electrode) to form an HNT-CNC-SWCNT / GCE working electrode. The method comprises the following steps: firstly, uniformly dispersing CNC and SWCNT which are equal in mass in an N, N-dimethylformamide solution through magnetic stirring in an ultrasonic environment; then, adding HNT into the solution, and carrying out ultrasonic dispersion to obtain a uniformly mixed HNT-coated CNC-coated SWCNT turbid liquid; and finally, dispensing the uniform turbid liquid on the surface of GCE, and drying under an infrared lamp to prepare the nano composite material modified HNT / CNC / SWCNT / GCE working electrode. Based on the synergistic interaction effect generated by HNT, CNC and SWCNT, the HNT / CNC / SWCNT / GCE sensor is high in sensitivity, the detection limit can reach 18.04 nM, and the diuron sensing detection performance is excellent.

The working electrode is characterized in that the working electrode is obtained by dispensing ferroferric oxide and lauric acid on the surface of a glassy carbon electrode. A working electrode for detecting the micro-plastic is a working electrode, an Ag / AgCl electrode is a reference electrode, and platinum is an auxiliary electrode; preparing a series of concentrations of micro-plastic standard sample solutions, preparing a ferrocene solution and an electrolyte, firstly putting a working electrode, a reference electrode and an auxiliary electrode into the standard sample solutions, incubating to reach adsorption equilibrium, then taking out the three electrodes, putting the three electrodes into the ferrocene solution, and incubating; and finally, taking out the three-electrode system, putting the three-electrode system into electrolyte, and carrying out DPV detection. A simple and low-cost electrochemical sensing detection method is established by utilizing the reaction of the micro-plastic reinforced ferrocene signal molecules. The method is rapid, simple and low in cost, and can effectively detect the micro-plastics in a wide range and maintain good sensitivity.

The invention discloses a hexachlorophenol electrochemical sensor built based on NiFe-coated C / MoO3 and a preparation method and application thereof.The hexachlorophenol electrochemical sensor comprises a working electrode, an auxiliary electrode and a reference electrode, the working electrode is a NiFe-coated C / MoO3 modified electrode and is composed of a glassy carbon electrode and a NiFe-coated C / MoO3 composite material modified on the glassy carbon electrode, the auxiliary electrode is a NiFe-coated C / MoO3 modified electrode, and the reference electrode is a NiFe-coated C / MoO3 modified electrode. The NiFe (at) C / MoO3 composite material comprises a NiFe (at) C framework and MoO3 loaded on the NiFe (at) C framework, and the NiFe (at) C framework is formed by embedding NiFe bimetal nanoparticles into a porous carbon framework. The preparation method comprises the steps of synthesis of MoO3, synthesis of NiFe C, preparation of the modified electrode and preparation of the electrochemical sensor. The hexachlorophenol electrochemical sensor disclosed by the invention can be used for direct electrochemical detection without derivatization, and is high in selectivity, portable at low cost, ultrahigh in sensitivity and good in application prospect.

The invention discloses a preparation method of a double-enzyme glassy carbon electrode, which comprises the following steps: placing a GCE electrode in a Piranha solution, cleaning the GCE electrode with distilled water, polishing the GCE electrode, and finally carrying out ultrasonic cleaning and drying. Carrying out activating treatment on the cleaned GCE electrode; the preparation method comprises the following steps: adding a mixture of tin dioxide and multi-walled carbon nanotube powder into a chitosan / acetic acid solution, and carrying out ultrasonic treatment, so as to obtain an MWCNTs-SnO2-CS nano compound; the preparation method comprises the following steps: modifying GCE by using a nano-composite to obtain GCE taking MWCNTs / SnO2 / CS as a modification material; choline oxidase and horse radish peroxidase are immobilized on GCE (Gelatin CE) which takes MWCNTs / SnO2 / CS as a modification material to prepare the double-enzyme GCE. The double-enzyme GCE prepared by the invention can be used for detecting the content of PC in crude oil, and a theoretical basis is provided for rapidly detecting the content of PC in crude oil.

The invention relates to the technical field of electrochemical analysis and detection, in particular to a sensitive detection tert-butylhydroquinone modified glassy carbon electrode, a preparation method and application, the sensitive detection tert-butylhydroquinone modified glassy carbon electrode has high specific surface area and catalytic activity and good conductivity, sensitive detection of tert-butylhydroquinone can be achieved, and the application range is wide. The modified glassy carbon electrode comprises a glassy carbon electrode and a compound coating, the compound coating wraps the surface of the glassy carbon electrode, and the compound coating is composed of graphene oxide and a Ti-based MOF derivative; the preparation method comprises the following steps: (1) preparing MIL-125 (Ti); (2) preparation of TiO2 / NC; (3) preparation of 15% GO (at) TiO2 / NC; (4) preparing a dispersion liquid of the prepared 15% GO-coated TiO2 / NC composite material; (5) manufacturing a modified electrode; the application comprises the following steps: S1, establishing a concentration-electrochemical response signal relationship; and S2, measuring the concentration of the to-be-measured substance.

The present invention provides a homocysteine ​​electrochemical sensor, and its preparation method and application. The preparation method of the homocysteine ​​electrochemical sensor of the present invention comprises the following steps: adding a dispersion containing carbon nanomaterials to the surface of a glassy carbon electrode, baking and drying, to obtain a carbon nanomaterial-modified electrode; then placing the carbon nanomaterial-modified electrode in a gold salt aqueous solution, and using an electroplating method to deposit nanogold on the surface of the carbon nanomaterial-modified electrode, to obtain a homocysteine ​​electrochemical sensor; the high conductivity of gold nanoparticles is of great significance for improving the electrocatalytic performance of the composite material for homocysteine. The homocysteine ​​electrochemical sensor prepared by the present application has low cost, low detection limit, and good stability. In addition, the spiked recovery rate of homocysteine ​​in serum samples is good, which provides potential application value for the reliable determination of homocysteine ​​in biological systems and the study of homocysteine-related diseases.

The invention relates to the technical field of biological detection, in particular to an MWCNTs-COOH / AuNPs / CS / Apt electrode modification material, an electrode and a preparation method and application of the MWCNTs-COOH / AuNPs / CS / Apt electrode modification material. A glassy carbon electrode is used as a substrate, and a carboxylated multi-walled carbon nanotube / gold nanoparticle / chitosan (MWCNTs-COOH / AuNPs / CS) nano composite material layer, an aptamer (Apt) recognition layer and a mercaptohexanol (MCH) closing layer are sequentially modified. The preparation method comprises the following steps: hydrothermally synthesizing AuNPs, preparing an MWCNTs-COOH / AuNPs / CS compound through ultrasonic dispersion, modifying the surface of an electrode layer by layer and the like. The aptamer immobilization technology and detection conditions are optimized, the sensor has high-sensitivity linear response to 8-OHdG within the range of 0.0706-7.06 mu M, the detection limit reaches 0.33 mu M, and the anti-interference performance is high (interference signal lt; 5%). The method solves the problems that a traditional detection method is high in cost and long in consumed time and an existing sensor is insufficient in sensitivity, and is suitable for rapid and accurate analysis of 8-OHdG in biomedical detection and environmental monitoring.

The invention belongs to the technical field of amino acid detection, and relates to an electrode for identifying and detecting cysteine and application, the electrode is a glassy carbon electrode modified by vitamin B12 and subjected to electrochemical activation treatment; the electrochemical activation treatment comprises oxidation treatment and reduction treatment; the vitamin B12 modification method is an impregnation method and / or the electrolyte in the reduction treatment process contains vitamin B12, and modification is carried out in the electrochemical activation process. According to the invention, direct electric polarization of the molecular catalyst is realized, Cys can be effectively distinguished from sulfydryl of other similar structures, and interference of common electroactive compounds coexisting in a biological sample is eliminated; the super-hydrophilic surface endows the sensor with excellent biological pollution resistance; a new example is provided for electrochemical sulfydryl analysis, an important theoretical basis is provided for bionic interface engineering, and the method is expected to be applied to clinical diagnosis and on-site rapid detection; and a thought is provided for modification of other types of molecular catalysts such as Co-N4.

The invention relates to an electrochemical sensor for detecting xanthine, the electrochemical sensor is a three-electrode system, a working electrode of the electrochemical sensor is prepared by preparing a modification solution from Cu-BTC-HHTP-HCG and dispensing the modification solution on the surface of a glassy carbon electrode, and the Cu-BTC-HHTP-HCG is synthesized from copper nitrate trihydrate, 1, 3, 5-benzene tricarboxylic acid, 2, 3, 6, 7, 10, 11-hexahydroxy triphenylene hydrate and high-conductivity graphene. According to the electrochemical sensor for xanthine detection, Cu-BTC-HHTP has high electrocatalytic activity, specific surface area and porosity, the enrichment efficiency and electron transfer capability of xanthine can be effectively improved, meanwhile, the conductivity and stability of the electrochemical sensor can be further enhanced through compounding of high-conductivity graphene, and the electrochemical sensor can be applied to xanthine detection. Therefore, the electrochemical sensor is endowed with excellent electrochemical response performance. The electrochemical sensor is high in sensitivity, low in detection limit, good in stability and interference resistance and suitable for detecting xanthine in actual fish and shrimp products.

The invention discloses a composite material modified electrode based on a functionalized single-walled carbon nanotube and application thereof, and belongs to the technical field of electrochemistry, the modified electrode comprises a glassy carbon electrode and a functionalized single-walled carbon nanotube-glucose composite material coating coated on the surface of the glassy carbon electrode; the mass ratio of functionalized single-walled carbon nanotubes to glucose in the coating is (0.2-5): 1, and the functionalized single-walled carbon nanotubes comprise at least one of hydroxylated single-walled carbon nanotubes, carboxylated single-walled carbon nanotubes and aminated single-walled carbon nanotubes, preferably hydroxylated single-walled carbon nanotubes. The composite material modified electrode provided by the invention has a large number of active sites, and by effectively utilizing the synergistic effect of the hydroxylated single-walled carbon nanotubes and glucose, the modified electrode shows excellent selective recognition capability on amino acid enantiomers and also shows good stability; and the method has a good application prospect in quantitative analysis of the amino acid enantiomers.

The invention belongs to the field of new materials, and discloses an electrode-based liposome adriamycin entrapment rate electrochemical detection method. According to the detection method, after a bipolar vertical ordered mesoporous silica film (bp-VMSF) is modified on an electrically activated glassy carbon electrode (p-GCE), separation, enrichment and electrochemical detection are integrated, and rapid and sensitive detection of adriamycin can be realized. The glassy carbon electrode is subjected to electrochemical activation pretreatment under the alkaline condition, bp-VMSF stably grows, the surface of the inner layer close to the p-GCE electrode is negatively charged, and the surface of the outer layer away from the electrode is positively charged. The asymmetric charge configuration enhances the adsorption capacity of positively charged adriamycin molecules on an electrode interface through electrostatic enrichment. The p-GCE adsorbs adriamycin and promotes electron transfer, and is combined with the synergistic enrichment effect of the bp-VMSF double-static nano-channel, so that the electrochemical response performance to adriamycin is jointly improved, a method is provided for rapid detection of the liposome adriamycin encapsulation efficiency, and the p-GCE has a good prospect in evaluation of liposome adriamycin preparations.

The invention provides a preparation method and application of an electrochemical luminescence aptamer sensor for detecting perfluorooctanoic acid (PFOA) in a water environment by adopting an apt / CHIT / Zn TCPP (at) MOF / GCE modified electrode based on an electrochemical luminescence method. The preparation method comprises the following steps: electrically depositing a zinc tetraphenylporphyrin functionalized metal organic framework (Zn TCPP-coated MOF / GCE) on a glassy carbon electrode (GCE), and further modifying an aptamer (apt) of chitosan (CHIT) and PFOA to prepare an apt / CHIT / Zn TCPP-coated MOF / GCE modified electrode. The modified electrode apt / CHIT / Zn TCPP (at) MOF / GCE prepared on the basis of an electrodeposition technology is low in detection limit and good in reproducibility, pretreatment of concentration and enrichment on a water environment sample is not needed due to high sensitivity, and analysis operation on PFOA is simple and easy to implement.

The invention discloses a method for synchronously detecting multiple heavy metal ions in a water body, relates to a method for synchronously detecting multiple heavy metal ions in a water body, and belongs to the technical field of environmental monitoring. The problems of poor selectivity and organic interference during synchronous detection of multiple heavy metal ions are solved. According to the scheme, three heterojunction modification layers are constructed on a glassy carbon electrode to form a bionic photoelectric electrode; step-by-step photoelectric enrichment: 650nm laser is adopted,-0.6 V enables a middle layer to capture Cd < 2 + > and Pb < 2 + >, 532nm laser is adopted,-1.5 V pulse enables a surface layer to enrich Cu < 2 + > and Hg < 2 + >, and square waves repel organic matters; dissolution peaks are collected in different areas in differential pulse dissolution scanning, 12 keV X-ray excitation is synchronized, and quantification is performed only when the target ion characteristic dissolution peak potential is matched with the corresponding characteristic X-ray fluorescence signal at the same time. The method is used for synchronously and accurately detecting Cd < 2 + >, Pb < 2 + >, Cu < 2 + > and Hg

The invention relates to the technical field of electrochemical detection, in particular to a preparation method of a polydopamine modified nitrogen-doped carbon material and application of the polydopamine modified nitrogen-doped carbon material in electrochemical analysis and detection of copper ions. The preparation method comprises the following steps: calcining a Zn-MOF material in an inert atmosphere, removing metal zinc to obtain a porous nitrogen-doped carbon material with a hexagonal structure, mixing the nitrogen-doped carbon material with dopamine, and carrying out oxidative polymerization on the dopamine under an alkaline condition to obtain the polydopamine-modified nitrogen-doped carbon material. The polydopamine-modified nitrogen-doped carbon material prepared by the method has high electrochemical activity, rich active sites and excellent adsorption performance, is used for modifying a glassy carbon electrode to detect Cu < 2 + >, has high sensitivity to Cu < 2 + >, and overcomes the technical defects of electrode materials in the prior art.

The application discloses a kind of glucose oxidase sensors based on modified multi-walled carbon nanotube modification, it is related to glucose oxidase sensor technical field, including the following steps: (1) modified multi-walled carbon nanotube preparation;(2) glassy carbon electrode is carried out surface polishing treatment;(3) obtain modified multi-walled carbon nanotube dispersion;(4) obtain glucose oxidase liquid;(5) glucose glucose oxidase liquid impregnation electrode;(6) using Nafion solution covers electrode;The sensor obtained after the electrode is modified by introducing modified multi-walled carbon nanotube in the application, not only has higher glucose detection sensitivity, but also has excellent stability.

The invention discloses a kit for detecting an A beta oligomer. The kit comprises a nucleic acid aptamer-activator modified streptavidin magnetic bead, a methylene blue nucleic acid signal probe modified streptavidin magnetic bead, a Cas12a-crRNA compound and a synthetic gold nanocluster probe modified glassy carbon electrode. According to the invention, the beta-CD-AuNCs probe with high quantum yield is used as an electrochemiluminescence material, methylene blue is used as an electrochemiluminescence quencher, and through combination with CRISPR / Cas technology, signal change of an ECL reaction system is effectively regulated and controlled, and AbetaO detection is realized. The method has the advantages of strong detection specificity, good selectivity, high sensitivity and strong anti-interference capability on AbetaO, and has good market value.

The invention relates to the technical field of detection and identification of fentanyl substances, and discloses a selective quantitative detection method of fentanyl. According to the method, a differential pulse voltammetry (DPV) is used for making a linear relation diagram of a potassium ferricyanide peak current and fentanyl solutions with different concentrations to obtain a standard working curve, a linear equation is obtained through the standard curve, and the detection limit is 1.0 * 10 <-12 > mol.L <-1 >; the molecularly imprinted membrane modified electrode prepared by the invention is used for measuring the peak current of a potassium ferricyanide solution, so that fentanyl molecules can be indirectly and quantitatively analyzed and measured. The method is characterized in that a fentanyl molecularly imprinted membrane is electrically polymerized on the surface of a glassy carbon electrode, m-phenylenediamine is used as a functional monomer, and the selectivity and sensitivity of an electrochemical method for detecting fentanyl are improved. Meanwhile, by taking an adsorption pulse stripping voltammetry (DPASV) as a detection technology, a method capable of rapidly detecting fentanyl molecules in the presence of various drugs is established. The molecularly imprinted membrane electrode disclosed by the invention is simple to prepare, quick in response and good in selectivity and reproducibility, and can be repeatedly used for more than five times.

The invention relates to the technical field of preparation of electrochemical sensors, and particularly discloses a preparation method of a novel electrochemical enzyme sensor for profenofos pesticide detection based on an enzyme inhibition action principle, and the preparation method comprises the following steps: S1, treating a glassy carbon electrode to obtain a bare glassy carbon electrode GCE; s2, obtaining a composite material modified electrode Au50Ag2 (at) G / GCE; s3, an enzyme-chitosan composite modified electrode KbE-CS / Au50Ag2 (at) G / GCE is obtained; and S4, finally obtaining the profenofos electrochemical enzyme sensor NF / KbE-CS / Au50Ag2 (at) G / GCE based on the enzyme inhibition effect. According to the present invention, the white kidney bean esterase derived from plants is adopted as the detection enzyme source, and the electro-catalysis amplification effect of the Au50Ag2 (at) G composite material is adopted to construct the novel electrochemical enzyme sensor, and the method is the profenofos pesticide detection technology based on the enzyme inhibition effect principle;

The present invention relates to the field of electrochemical sensing technology, and particularly to a method for preparing an efficient and sensitive NHDC electrochemical sensor and its application. A two-step drop-coating method is adopted. First, the CuO dispersion is drop-coated on the electrode surface, and then the ZIF-8@CNTs dispersion is continuously drop-coated on the electrode surface to prepare a CuO / ZIF-8@CNTs double-layer composite film modified electrode, thereby constructing a rapid and sensitive NHDC electrochemical sensor. The present invention utilizes the high electrocatalytic activity of the CuO material, the high specific surface area and high porosity of the ZIF-8 material, and the high conductivity of the CNTs to prepare a CuO / ZIF-8@CNTs double-layer composite film, and then compositely modifies it on the surface of the glassy carbon electrode. The synergistic effect of the three enhances the electrochemical response signal of NHDC on its electrode surface, and there is a good linear relationship between the oxidation current of NHDC and the concentration within a certain concentration range, with a low detection limit.