439 results about "Glassy carbon electrode" patented technology

The invention discloses an all-solid-state calibration-free ion concentration detection method. The method comprises the following steps: constructing a plurality of traditional three-electrode detection systems; in each traditional three-electrode detection system, current and impedance signals are synchronously acquired through a step potential electrochemical spectrum and an electrochemical impedance spectrum, and interface kinetic parameters are analyzed; on the basis of a traditional three-electrode detection system, an original reference electrode is removed, a counter electrode is reserved, all glassy carbon electrodes with gradient membrane volumes are connected in series one by one to obtain a new three-electrode detection system, the new three-electrode detection system is immersed in a to-be-detected solution, a preset potential is applied, and through transient current response in combination with calibrated kinetic parameters, the gradient membrane volumes of the glassy carbon electrodes are detected. And performing inversion to obtain the ion concentration of the to-be-detected solution. According to the method, automatic calibration is realized by analyzing interface process kinetic parameters of different-volume film ion selective electrodes and calculating the slope to generate a built-in standard curve. According to the method, interface process parameters are obtained through transient current, and dependence of a traditional detection system on the stable potential of a reference electrode is eliminated.

The invention discloses a nitrite electrochemical sensor based on a graphene oxide and polytromethamine composite material and a preparation method and application thereof, and belongs to the field of electrochemical method detection, the preparation method comprises the following steps: uniformly dispensing a graphene oxide suspension on the surface of a glassy carbon electrode, and airing to obtain GO / GCE; putting the GO / GCE, a saturated calomel electrode and a platinum wire electrode into an electrolyte containing tromethamine with a certain concentration, and carrying out electropolymerization by adopting a cyclic voltammetry to obtain Poly (Tris) / GO / GCE; a saturated calomel electrode is used as a reference electrode, a platinum wire electrode is used as a counter electrode, Poly (Tris) / GO / GCE is used as a working electrode, and the nitrite electrochemical sensor is constructed and is more accurate and sensitive in nitrite detection, the linear detection range is 2 * 10 <-6 >-6 * 10 <-3 > mol / L, and the detection limit is 1.00 * 10 <-7 > mol / L.

The invention belongs to the field of electrochemical sensing, and particularly relates to construction and application of an electrochemical luminescence sensor for detecting the content of N-nitrosodimethylamine with high sensitivity. The Cas12a enzyme improves the specificity and realizes signal amplification through the recognition mechanism and trans-cleavage capability of the Cas12a enzyme. The ATT-AuNCs is used as an efficient electrochemical luminescence emitter, so that the transition of a signal is promoted. After a sample is added, the Cas12a-crRNA compound can specifically recognize a DNA activator to trigger cutting of non-specific DNA which is modified on a glassy carbon electrode and is marked by ferrocene, so that the electrochemical luminescence intensity is increased. The NDMA in the sample can be detected through the method, so that the content of nitrosamine in food can be detected. The CRISPR / Cas12a is combined with an electrochemical luminescence technology, so that a sensitive and rapid NDMA detection means is provided, and a new idea is provided for detecting the content of cancerogenic substances in various foods.

The invention discloses a biosensor for detecting Alzheimer's disease markers as well as a construction method and application thereof, and relates to the technical field of biosensors. The construction method comprises the following steps: after a glassy carbon electrode is cleaned, dropwise adding a RuSi NPs (at) CS compound on the surface of the glassy carbon electrode to obtain a RuSi NPs (at) CS / GCE electrode; and immersing the RuSi NPs coated CS / GCE electrode into a PHL: S double-chain solution, and carrying out an incubation reaction to obtain the biosensor. The RuSi NPs (at) CS compound is obtained by carrying out mixed reaction on ruthenium-silicon nanoparticles and chitosan; pHL: S double chains contained in the PHL: S double-chain solution are obtained by carrying out incubation reaction on carboxyl-activated PHL chains and S chains. The biosensor has the advantages of high accuracy, strong sensitivity, good selectivity and the like, and can be applied to accurate detection of the Alzheimer's disease marker A beta oligomer.

The invention provides a preparation method of a plate-shaped glassy carbon electrode, which comprises the following steps: curing a resin raw material into a plate-shaped resin block, carbonizing in an inert atmosphere, and respectively introducing a nitrogen source and a boron source for doping operation to obtain a co-doped plate-shaped resin block precursor; placing the co-doped plate-shaped resin block between two porous graphite plates, and carrying out high-temperature treatment to obtain a glassy carbon matrix; and immersing the glassy carbon substrate into the adhesion layer solution to form an adhesion layer, transferring the adhesion layer into a deposition solution which comprises a bismuth-containing solution and a gold-containing solution, forming bismuth-gold nano-dots on the adhesion layer, and washing and drying the bismuth-gold nano-dots to obtain the plate-shaped glassy carbon electrode. Through directional arrangement of crystal orientations, the tantalum carbide coating achieves higher electrical conductivity and thermal conductivity. And the sensitivity and the service life of the plate-shaped glassy carbon electrode are obviously improved and prolonged.

The invention discloses a dopamine sensor working electrode based on an MOF material and a preparation method thereof, and belongs to the technical field of electrochemical sensing. The working electrode is composed of a pure MOF Cu-HHTP modified glassy carbon electrode and a Nafion film covering the surface of the glassy carbon electrode, and the preparation method of the working electrode comprises the following steps: synthesizing Cu-HHTP (Cu < 2 + > and a hexahydroxy triphenyl ligand are coordinated to form a two-dimensional honeycomb layer) through a solvothermal method, dispensing Cu-HHTP dispersion liquid on the surface of the glassy carbon electrode, and then covering the glassy carbon electrode with the Nafion film to form the combined electrode. The dopamine sensor prepared by the preparation method disclosed by the invention has high sensitivity, high anti-interference capability and long-term stability. The preparation process is simple, complex conductive additives are not needed, and the method is suitable for rapid and accurate detection of dopamine in complex biological samples.

The invention relates to a water quality detection method for detecting heavy metal elements in water, which comprises the following steps: S1, pretreating a water sample, and filtering to remove suspended particles and impurities; s2, acid digestion of the sample: digesting the sample by using a mixed solution of nitric acid and hydrochloric acid to release heavy metal elements; s3, electrochemical sensor detection: anodic stripping voltammetry (ASV) or differential pulse voltammetry (DPV) is adopted for detection, and the sensor uses a gold-plated electrode or a glassy carbon electrode modified with carbon nanotubes and graphene; s4, concentration calculation: calculating the heavy metal concentration according to a current peak value and a volume correction formula; and S5, verifying a result, and rechecking a detection result by adopting an ICP-MS or AAS method. The method is high in sensitivity, can accurately detect trace heavy metals in drinking water, industrial wastewater, surface water and underground water, is suitable for simultaneous detection of multiple elements, has the advantages of simplicity and convenience in operation, portability of equipment, high detection speed and the like, and is particularly suitable for on-site water quality monitoring.

The invention relates to the technical field of electrochemical immunosensors, in particular to a preparation method and application of an ultra-sensitive electrochemical immunosensor for detecting MX1 protein based on a nano composite material, and the preparation method comprises the following steps: S1, pretreating a glassy carbon electrode; s2, modifying with thiolated graphene oxide; s3, gold nanoparticle modification; s4, carrying out antibody immobilization; and S5, carrying out bovine serum albumin sealing treatment. The kit has ultrahigh sensitivity and high specificity, the detection method is simple, convenient and rapid, multiple signal amplification mechanisms and wide clinical application prospects are achieved, and early diagnosis and clinical monitoring of virus infection are facilitated.

The invention belongs to the technical field of seawater detection, and particularly relates to a detection method for simultaneously detecting seawater-based Pb (II) and Cd (II), which comprises the following steps: step 1, polishing a glassy carbon electrode: polishing the glassy carbon electrode for three times by using aluminum oxide powder according to an 8-shaped track, then carrying out ultrasonic treatment in ultrapure water for 2-4 minutes, removing surface adhesion substances, washing by using ultrapure water, and drying to obtain the glassy carbon electrode; and activating the glassy carbon electrode in a sulfuric acid solution, scanning by using cyclic voltammetry until a stable cyclic voltammetry curve is obtained, washing by using ultrapure water, and drying at room temperature. Step 2, preparing a graphitized carboxylated multi-walled carbon nanotube solution, namely weighing 3.5 mg of graphitized carboxylated multi-walled carbon nanotubes and 0.8 mg of nano zinc oxide, dissolving the graphitized carboxylated multi-walled carbon nanotubes and the nano zinc oxide in 5ml of dimethylformamide, and performing ultrasonic dispersion for more than 3h to obtain the graphitized carboxylated multi-walled carbon nanotube solution; the method has good stability, reproducibility and anti-interference performance, and can realize high-sensitivity detection of Pb (II) and Cd (II) in actual seawater.

The invention provides a lactic acid electrochemical sensor LDHamp; the invention relates to construction of BiNPs (at) MWCNTs / GCE and a serum lactic acid detection method, and belongs to the technical field of biosensors, the method comprises the following steps: taking sodium citrate, Bi (NO3) 35H2O and NaBH4 as raw materials, synthesizing BiNPs through a multi-step reaction, compounding the BiNPs with MWCNTs, carrying out carboxyl activation and loading LDH, and finally modifying on a glassy carbon electrode to obtain the sensor. During detection, the sensor is placed in a PBS system containing 5mM of NAD, a serum sample is added, and the concentration of lactic acid is measured by electrochemical scanning according to current response change. According to the method, the detection linear range is 0-200 [mu] M, the detection limit is as low as 0.03 [mu] M, the detection difference of different batches of sensors is small, the method shows the advantages of high sensitivity and good stability, meanwhile, efficient and accurate detection of lactic acid in serum is achieved, and powerful support is provided for clinical diagnosis and disease research.

The invention relates to a samarium molybdate / reduced graphene oxide electro-catalytic material, a preparation method and application thereof and an electrochemical sensor, and relates to the technical field of electro-catalytic materials and electrochemical sensors, the preparation method comprises the following steps: dispersing GO in water, and stirring to obtain a GO suspension; the preparation method comprises the following steps: separately adding Sm (NO3) 3.6 H2O and Na2MoO4. 2H2O into water for ultrasonic dissolution, and mixing to obtain a first mixture; stirring and mixing the GO suspension and the first mixture to obtain a second mixture, and the like. The samarium molybdate / reduced graphene oxide electro-catalytic material is synthesized by a hydrothermal synthesis method and is used for modifying a glassy carbon electrode so as to be applied to electrochemical detection of FZD for the first time. Compared with a traditional detection method, the detection method has the advantages of being simple, efficient, green, environmentally friendly, sensitive in response and portable, and a new way is provided for detection of carcinogenic residues.

The invention provides an electrochemical sensor for identifying amino acid enantiomers and a preparation method and application thereof, and relates to the technical field of electrochemistry, the sensor comprises a glassy carbon electrode and a xylose-inulin composite coating coated on the glassy carbon electrode. The preparation method comprises the following steps: S1, preparing the xylose-inulin composite material in a self-assembly manner; and S2, dispensing the xylose-inulin composite material obtained in S1 on the surface of a glassy carbon electrode, and airing to obtain the xylose-inulin modified glassy carbon electrode. The glassy carbon electrode modified by the composite material has a large number of active sites, and the modified electrode shows selective recognition on amino acid enantiomers by effectively utilizing the synergistic effect of xylose and inulin. The electrode disclosed by the invention has good stability, specificity and chiral recognition capability on the amino acid enantiomers, and has good application prospects in quantitative analysis of the amino acid enantiomers.

The invention relates to the technical field of organic synthesis, in particular to 1-chloro-8-fluorine-7-isopropyl benzofuro [2, 3-c] pyridine as well as a synthesis method and an application of the 1-chloro-8-fluorine-7-isopropyl benzofuro [2, 3-c] pyridine. According to the invention, 1-chloro-8-fluoro-7-isopropyl benzofuro [2, 3-c] pyridine and nanogold are cooperated to cover the surface of a glassy carbon electrode (GCE), and a novel electrochemical modified electrode AuNPs / 1 / GCE is developed. The modified glassy carbon electrode shows good selectivity, high stability and sensitivity and wide detection range on the detection of the antibiotic furacilin.

The invention relates to the technical field of electrochemical sensing, in particular to an MXene-coated MB / PtNPs electrochemical sensor and a preparation method thereof and a carbendazim detection method, MXene is used as a carrier, PtNPs is embedded between layers and on the surface of MXene through reduction reaction, then electroactive molecules MB are introduced through electrostatic interaction, an MXene-coated MB / PtNPs composite material is formed, and the MXene-coated MB / PtNPs electrochemical sensor is prepared. And then modifying a glassy carbon electrode by using the MXene coated MB / PtNPs composite material to prepare the MXene coated MB / PtNPs electrochemical sensor. The compound is used as a ratio electrochemical sensor for detecting carbendazim, the linear range of the compound to carbendazim is 10-30000 ng / mL, the detection limit is 3.3 ng / mL, the detection speed is high, the sensitivity is high, the linear range is wide, the precision is high, the detection limit is low, meanwhile, the anti-interference performance and repeatability are good, and the compound has potential application prospects in the field of agricultural product quality detection.

The invention relates to the technical field of electrochemical luminescence detection, in particular to an electrochemical luminescence aptamer sensor based on a CoOOH-cDNA / Apt / AgNWs / Tb (at) A-COF / GCE working electrode. The iBF in a sample to be detected is detected by utilizing LC-MS / MS, GC-MS, UHPLC and HPLC-MS, so that the cost is high, the speed is low, and the sensitivity is relatively low. Aiming at the technical problem, the invention provides the CoOOH-cDNA / Apt / AgNWs / Tb-coated A-COF / GCE working electrode, the working electrode takes Tb-coated A-COF as a donor and CoOOH NSs as a receptor, the donor and the receptor are combined and modified on a glassy carbon electrode (GCE) through an aptamer and cDNA, then the CoOOH-cDNA / Apt / AgNWs / Tb-coated A-COF / GCE is taken as the working electrode, and iBF in a sample to be detected is quantitatively detected. According to the invention, through a signal "on-off-on" (on-off-on) mode, the consistency of detection results is significantly improved, the generation of false positive or false negative signals is reduced, and the detection accuracy is improved.

The invention belongs to the field of electrochemical sensors, and relates to an electrochemical sensor for high-sensitivity detection of carbendazim and a preparation method of the electrochemical sensor. The surface of a glassy carbon electrode (GCE) is modified with an LDMS-CCN-GCT nanocomposite material prepared from dendritic large-aperture mesoporous silica, conductive carbon black nanoparticles and graphitized carbon nanotubes, and a CCN-GCT-LDMS / GCE working electrode is formed. The preparation method comprises the following steps: firstly, uniformly dispersing CCN and GCT in an N, N-dimethylformamide solution through magnetic stirring in an ultrasonic environment, wherein the CCN and the GCT are equal in mass; then, adding LDMS into the solution, and carrying out ultrasonic dispersion to obtain a uniformly mixed CCN (at) GCT (at) LDMS suspension; and finally, dispensing the uniform suspension onto the surface of GCE, and drying under an infrared lamp to prepare the working electrode CCN (at) GCT (at) LDMS / GCE modified by the CCN (at) GCT (at) LDMS nano composite material. Based on the synergistic effect generated by LDMS and CCN-coated GCT, the CCN-coated GCT-coated LDMS / GCE sensor is high in sensitivity, the detection limit can reach 7.36 nM, and the CCN-coated GCT-coated LDMS / GCE sensor has excellent carbendazim sensing detection performance.

The invention discloses a bimetallic / carbon nanotube compound modified glassy carbon electrode as well as a preparation method and application thereof, and belongs to the technical field of electrochemical analysis. The bimetallic / carbon nanotube compound modified glassy carbon electrode provided by the invention comprises a glassy carbon electrode substrate and a bimetallic / carbon nanotube compound compounded on the glassy carbon electrode substrate, the bimetallic / carbon nanotube compound is prepared from nickel-cobalt bimetallic nanoparticles and a multi-walled carbon nanotube. The prepared bimetallic / carbon nanotube compound modified glassy carbon electrode has excellent electrocatalytic activity, has the advantages of good stability, low detection limit and high sensitivity and selectivity in the process of determining paracetamol, can realize on-site rapid detection of paracetamol, has the advantages of being convenient to carry and simple to operate, and has a wide application prospect. Wide application prospects are realized in medicine safety and clinical medical treatment.

The invention belongs to the field of electrochemical sensors, and relates to an electrochemical sensor for high-sensitivity detection of diuron and a preparation method of the electrochemical sensor. An HNT (halloysite nanotube)-CNC (carbon nanocage)-SWCNT (single-walled carbon nanotube) nanocomposite material prepared from a halloysite nanotube, a carbon nanocage (CNC) and an SWCNT (single-walled carbon nanotube) is modified on the surface of a GCE (glassy carbon electrode) to form an HNT-CNC-SWCNT / GCE working electrode. The method comprises the following steps: firstly, uniformly dispersing CNC and SWCNT which are equal in mass in an N, N-dimethylformamide solution through magnetic stirring in an ultrasonic environment; then, adding HNT into the solution, and carrying out ultrasonic dispersion to obtain a uniformly mixed HNT-coated CNC-coated SWCNT turbid liquid; and finally, dispensing the uniform turbid liquid on the surface of GCE, and drying under an infrared lamp to prepare the nano composite material modified HNT / CNC / SWCNT / GCE working electrode. Based on the synergistic interaction effect generated by HNT, CNC and SWCNT, the HNT / CNC / SWCNT / GCE sensor is high in sensitivity, the detection limit can reach 18.04 nM, and the diuron sensing detection performance is excellent.

The working electrode is characterized in that the working electrode is obtained by dispensing ferroferric oxide and lauric acid on the surface of a glassy carbon electrode. A working electrode for detecting the micro-plastic is a working electrode, an Ag / AgCl electrode is a reference electrode, and platinum is an auxiliary electrode; preparing a series of concentrations of micro-plastic standard sample solutions, preparing a ferrocene solution and an electrolyte, firstly putting a working electrode, a reference electrode and an auxiliary electrode into the standard sample solutions, incubating to reach adsorption equilibrium, then taking out the three electrodes, putting the three electrodes into the ferrocene solution, and incubating; and finally, taking out the three-electrode system, putting the three-electrode system into electrolyte, and carrying out DPV detection. A simple and low-cost electrochemical sensing detection method is established by utilizing the reaction of the micro-plastic reinforced ferrocene signal molecules. The method is rapid, simple and low in cost, and can effectively detect the micro-plastics in a wide range and maintain good sensitivity.

The invention discloses a hexachlorophenol electrochemical sensor built based on NiFe-coated C / MoO3 and a preparation method and application thereof.The hexachlorophenol electrochemical sensor comprises a working electrode, an auxiliary electrode and a reference electrode, the working electrode is a NiFe-coated C / MoO3 modified electrode and is composed of a glassy carbon electrode and a NiFe-coated C / MoO3 composite material modified on the glassy carbon electrode, the auxiliary electrode is a NiFe-coated C / MoO3 modified electrode, and the reference electrode is a NiFe-coated C / MoO3 modified electrode. The NiFe (at) C / MoO3 composite material comprises a NiFe (at) C framework and MoO3 loaded on the NiFe (at) C framework, and the NiFe (at) C framework is formed by embedding NiFe bimetal nanoparticles into a porous carbon framework. The preparation method comprises the steps of synthesis of MoO3, synthesis of NiFe C, preparation of the modified electrode and preparation of the electrochemical sensor. The hexachlorophenol electrochemical sensor disclosed by the invention can be used for direct electrochemical detection without derivatization, and is high in selectivity, portable at low cost, ultrahigh in sensitivity and good in application prospect.

The invention discloses a preparation method of a double-enzyme glassy carbon electrode, which comprises the following steps: placing a GCE electrode in a Piranha solution, cleaning the GCE electrode with distilled water, polishing the GCE electrode, and finally carrying out ultrasonic cleaning and drying. Carrying out activating treatment on the cleaned GCE electrode; the preparation method comprises the following steps: adding a mixture of tin dioxide and multi-walled carbon nanotube powder into a chitosan / acetic acid solution, and carrying out ultrasonic treatment, so as to obtain an MWCNTs-SnO2-CS nano compound; the preparation method comprises the following steps: modifying GCE by using a nano-composite to obtain GCE taking MWCNTs / SnO2 / CS as a modification material; choline oxidase and horse radish peroxidase are immobilized on GCE (Gelatin CE) which takes MWCNTs / SnO2 / CS as a modification material to prepare the double-enzyme GCE. The double-enzyme GCE prepared by the invention can be used for detecting the content of PC in crude oil, and a theoretical basis is provided for rapidly detecting the content of PC in crude oil.

The invention relates to the technical field of electrochemical analysis and detection, in particular to a sensitive detection tert-butylhydroquinone modified glassy carbon electrode, a preparation method and application, the sensitive detection tert-butylhydroquinone modified glassy carbon electrode has high specific surface area and catalytic activity and good conductivity, sensitive detection of tert-butylhydroquinone can be achieved, and the application range is wide. The modified glassy carbon electrode comprises a glassy carbon electrode and a compound coating, the compound coating wraps the surface of the glassy carbon electrode, and the compound coating is composed of graphene oxide and a Ti-based MOF derivative; the preparation method comprises the following steps: (1) preparing MIL-125 (Ti); (2) preparation of TiO2 / NC; (3) preparation of 15% GO (at) TiO2 / NC; (4) preparing a dispersion liquid of the prepared 15% GO-coated TiO2 / NC composite material; (5) manufacturing a modified electrode; the application comprises the following steps: S1, establishing a concentration-electrochemical response signal relationship; and S2, measuring the concentration of the to-be-measured substance.

The present invention provides a homocysteine ​​electrochemical sensor, and its preparation method and application. The preparation method of the homocysteine ​​electrochemical sensor of the present invention comprises the following steps: adding a dispersion containing carbon nanomaterials to the surface of a glassy carbon electrode, baking and drying, to obtain a carbon nanomaterial-modified electrode; then placing the carbon nanomaterial-modified electrode in a gold salt aqueous solution, and using an electroplating method to deposit nanogold on the surface of the carbon nanomaterial-modified electrode, to obtain a homocysteine ​​electrochemical sensor; the high conductivity of gold nanoparticles is of great significance for improving the electrocatalytic performance of the composite material for homocysteine. The homocysteine ​​electrochemical sensor prepared by the present application has low cost, low detection limit, and good stability. In addition, the spiked recovery rate of homocysteine ​​in serum samples is good, which provides potential application value for the reliable determination of homocysteine ​​in biological systems and the study of homocysteine-related diseases.

Electrochemical sensors based on metal cluster composites and methods of manufacture are provided. [Solution] Au 25 The graphene composite solution, the bean esterase chitosan solution, and the Nafion solution are dropped onto a glassy carbon electrode and then dried to obtain an electrochemical biosensor electrode. The metal cluster is composed of 25 gold atoms and a well-defined number of organic ligands, and has a precise composition and stable geometric structure. Au 25 The graphene composite has excellent electrical conductivity and cooperative electrocatalytic effect, which can effectively promote the transfer of electrons and improve the response sensitivity of the sensor. The electrochemical sensor can measure the content of profenofos by enzyme inhibition method using kidney bean esterase as the enzyme source.

The invention relates to the technical field of biological detection, in particular to an MWCNTs-COOH / AuNPs / CS / Apt electrode modification material, an electrode and a preparation method and application of the MWCNTs-COOH / AuNPs / CS / Apt electrode modification material. A glassy carbon electrode is used as a substrate, and a carboxylated multi-walled carbon nanotube / gold nanoparticle / chitosan (MWCNTs-COOH / AuNPs / CS) nano composite material layer, an aptamer (Apt) recognition layer and a mercaptohexanol (MCH) closing layer are sequentially modified. The preparation method comprises the following steps: hydrothermally synthesizing AuNPs, preparing an MWCNTs-COOH / AuNPs / CS compound through ultrasonic dispersion, modifying the surface of an electrode layer by layer and the like. The aptamer immobilization technology and detection conditions are optimized, the sensor has high-sensitivity linear response to 8-OHdG within the range of 0.0706-7.06 mu M, the detection limit reaches 0.33 mu M, and the anti-interference performance is high (interference signal lt; 5%). The method solves the problems that a traditional detection method is high in cost and long in consumed time and an existing sensor is insufficient in sensitivity, and is suitable for rapid and accurate analysis of 8-OHdG in biomedical detection and environmental monitoring.

The invention belongs to the technical field of amino acid detection, and relates to an electrode for identifying and detecting cysteine and application, the electrode is a glassy carbon electrode modified by vitamin B12 and subjected to electrochemical activation treatment; the electrochemical activation treatment comprises oxidation treatment and reduction treatment; the vitamin B12 modification method is an impregnation method and / or the electrolyte in the reduction treatment process contains vitamin B12, and modification is carried out in the electrochemical activation process. According to the invention, direct electric polarization of the molecular catalyst is realized, Cys can be effectively distinguished from sulfydryl of other similar structures, and interference of common electroactive compounds coexisting in a biological sample is eliminated; the super-hydrophilic surface endows the sensor with excellent biological pollution resistance; a new example is provided for electrochemical sulfydryl analysis, an important theoretical basis is provided for bionic interface engineering, and the method is expected to be applied to clinical diagnosis and on-site rapid detection; and a thought is provided for modification of other types of molecular catalysts such as Co-N4.

The invention relates to an electrochemical sensor for detecting xanthine, the electrochemical sensor is a three-electrode system, a working electrode of the electrochemical sensor is prepared by preparing a modification solution from Cu-BTC-HHTP-HCG and dispensing the modification solution on the surface of a glassy carbon electrode, and the Cu-BTC-HHTP-HCG is synthesized from copper nitrate trihydrate, 1, 3, 5-benzene tricarboxylic acid, 2, 3, 6, 7, 10, 11-hexahydroxy triphenylene hydrate and high-conductivity graphene. According to the electrochemical sensor for xanthine detection, Cu-BTC-HHTP has high electrocatalytic activity, specific surface area and porosity, the enrichment efficiency and electron transfer capability of xanthine can be effectively improved, meanwhile, the conductivity and stability of the electrochemical sensor can be further enhanced through compounding of high-conductivity graphene, and the electrochemical sensor can be applied to xanthine detection. Therefore, the electrochemical sensor is endowed with excellent electrochemical response performance. The electrochemical sensor is high in sensitivity, low in detection limit, good in stability and interference resistance and suitable for detecting xanthine in actual fish and shrimp products.

The invention discloses a composite material modified electrode based on a functionalized single-walled carbon nanotube and application thereof, and belongs to the technical field of electrochemistry, the modified electrode comprises a glassy carbon electrode and a functionalized single-walled carbon nanotube-glucose composite material coating coated on the surface of the glassy carbon electrode; the mass ratio of functionalized single-walled carbon nanotubes to glucose in the coating is (0.2-5): 1, and the functionalized single-walled carbon nanotubes comprise at least one of hydroxylated single-walled carbon nanotubes, carboxylated single-walled carbon nanotubes and aminated single-walled carbon nanotubes, preferably hydroxylated single-walled carbon nanotubes. The composite material modified electrode provided by the invention has a large number of active sites, and by effectively utilizing the synergistic effect of the hydroxylated single-walled carbon nanotubes and glucose, the modified electrode shows excellent selective recognition capability on amino acid enantiomers and also shows good stability; and the method has a good application prospect in quantitative analysis of the amino acid enantiomers.

The invention belongs to the field of new materials, and discloses an electrode-based liposome adriamycin entrapment rate electrochemical detection method. According to the detection method, after a bipolar vertical ordered mesoporous silica film (bp-VMSF) is modified on an electrically activated glassy carbon electrode (p-GCE), separation, enrichment and electrochemical detection are integrated, and rapid and sensitive detection of adriamycin can be realized. The glassy carbon electrode is subjected to electrochemical activation pretreatment under the alkaline condition, bp-VMSF stably grows, the surface of the inner layer close to the p-GCE electrode is negatively charged, and the surface of the outer layer away from the electrode is positively charged. The asymmetric charge configuration enhances the adsorption capacity of positively charged adriamycin molecules on an electrode interface through electrostatic enrichment. The p-GCE adsorbs adriamycin and promotes electron transfer, and is combined with the synergistic enrichment effect of the bp-VMSF double-static nano-channel, so that the electrochemical response performance to adriamycin is jointly improved, a method is provided for rapid detection of the liposome adriamycin encapsulation efficiency, and the p-GCE has a good prospect in evaluation of liposome adriamycin preparations.

The invention provides a preparation method and application of an electrochemical luminescence aptamer sensor for detecting perfluorooctanoic acid (PFOA) in a water environment by adopting an apt / CHIT / Zn TCPP (at) MOF / GCE modified electrode based on an electrochemical luminescence method. The preparation method comprises the following steps: electrically depositing a zinc tetraphenylporphyrin functionalized metal organic framework (Zn TCPP-coated MOF / GCE) on a glassy carbon electrode (GCE), and further modifying an aptamer (apt) of chitosan (CHIT) and PFOA to prepare an apt / CHIT / Zn TCPP-coated MOF / GCE modified electrode. The modified electrode apt / CHIT / Zn TCPP (at) MOF / GCE prepared on the basis of an electrodeposition technology is low in detection limit and good in reproducibility, pretreatment of concentration and enrichment on a water environment sample is not needed due to high sensitivity, and analysis operation on PFOA is simple and easy to implement.