1878 results about "Glassy carbon electrode" patented technology

The invention provides a load-type monoatomic catalyst. The catalyst is formed by uniformly loading monodispersed metal atoms on the surface of a nano substrate material. A preparation method thereofcomprises the following steps: in an electrolyte solution containing metal salt, performing electrochemical deposition by using a three-electrode system, using a glassy carbon electrode loaded with the nano substrate material as a working electrode, using a graphite rod as a counter electrode, using a silver/silver chloride electrode as a reference electrode, performing linear volt-ampere scanning, enabling the metal atoms to be monodispersely and uniformly deposited on the nano substrate material, to obtain the load-type monoatomic catalyst. The load-type monoatomic catalyst is capable of, based on electro-deposition, constructing a monoatomic structure, through an electro-deposition method, rapidly, efficiently, and controllably depositing single atoms, and realizing deposition of positive and negative electrodes, and the obtained load-type monoatomic catalyst has important significance to a general application in the catalytic and particle physical field.

The invention discloses preparation and application of magnetic nano-composite material gamma-Fe2O3/PDA (Polydopamine)-GA (Gallic Acid). The preparation comprises the following steps of carrying out a hydrothermal reaction and calcinations to obtain a gamma-Fe2O3 nano particle carrier by taking FeCl3.6H2O with low price as an iron source, ethylene glycol (EG) as a reducing agent, polyvinylpyrrolidone (PVP) as a dispersing agent and a protective agent, and natrium aceticum (NaAc) as an alkali source; then wrapping a layer of polydopamine (PDA) in a buffer solution of tris (hydroxymethyl) aminomethane-hydrochloric acid (Tris-HCl) with pH of 8.5; and finally introducing gallic acid (GA) to an ethanol system by using 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and n-hydroxysuccinimide as cross-linking agents, wherein a specific synthetic schematic diagram is seen in attached drawing 1. Then the adsorption effects of the synthesized magnetic nano-particle on metal ions Cu<2+> and Pb<2+> are discussed, the detection effects of a glassy carbon electrode modified by the magnetic nano-particle on the metal ions Cu<2+> and Pb<2+> are researched. Results show that gamma-Fe2O3/PDA-GA has excellent detection and adsorption effects on heavy metal ions in a water environment.

The present invention relates to a preparation method for a copper oxide-graphene nano-complex modification glassy carbon electrode, and an application of the electrode as a glucose electrochemical sensor in rapid glucose detection, and belongs to the technical field of electrochemical analysis detection. According to the present invention, an electrochemical catalytic oxidation effect is adopted, and a current-time curve method is adopted to carry out sensitive quantitative analysis determination on the glucose, wherein the electrochemical catalytic oxidation effect is provided by the glucose sensor, and the copper oxide-graphene nano-composite material is adopted to modify the glassy carbon electrode to prepare the glucose sensor. The key point of the invention is: coating a naphthol suspension of the graphene on the surface of the treated glassy carbon electrode in a dropwise manner; carrying out open-air drying to form a layer of uniform modification layer; and adopting an electrodeposition method with a characteristic of simple operation to prepare a sensitive layer of the copper oxide-graphene nano-complex to achieve the stable catalysis of the glucose oxidation. The prepared glucose sensor of the present invention can be used clinically in detection analysis of blood glucose content in serum, and the determination process has characteristics of rapidness, sensitivity, accuracy, stability, low interference and the like.

The invention provides a NiAl-laminated type bimetal hydroxide/carbon nano-tube compound electrode, a preparation method thereof, and an application of the electrode to the electro-catalytic oxidation of glucose. In the electrode, a glassy carbon electrode is taken as a substrate electrode and coated with a layer of film of a NiAl-laminated type bimetal hydroxide/carbon nano-tube compound. The preparation method comprises the following steps of: enabling laminated type bimetal hydroxide to grow in-situ on the surface of carbon nano-tubes by adopting a coprecipitation method to obtain the laminated type bimetal hydroxide/carbon nano-tube compound; then dispersing the compound into a water solution to prepare a solution; dispensing the solution on the surface of the clean glassy carbon electrode; drying at room temperature to volatilize the solvent and obtain the electrode modified by the laminated type bimetal hydroxide/carbon nano-tube compound. The electrode can be used for the electro-catalytic oxidation of the glucose under an alkaline condition and has favorable electro-catalysis performance and stability with simple synthesis method, low cost and wide application prospect.

The invention discloses a preparation method of a magnetic sandwich nano immunosensor and application of the preparation method. The preparation method is characterized by comprising the following steps: mixing and stirring a collaurum solution and silane ferriferrous oxide into a colorless and transparent solution, and carrying out magnetic separation by externally applying a magnet to obtain FE3O4/Au colloid nano magnetic beads; loading horse radish peroxidase (HRP) and secondary antibodies of an object to be measured to the FE3O4/Au colloid nano magnetic beads to obtain a secondary antibody probe Fe3O4/Au-HRP-Ab2; electrically depositing Au to a glassy carbon electrode, loading primary antibodies of the object to be measured, and then closing non-specific active sites by using protein to obtain an Abl/Au/GCE electrode; and finally, loading antigen to the Abl/Au/GCE electrode, and then obtaining the sandwich nano immunosensor by combining the magnet probe. The magnetic sandwich nano immunosensor can be used for measuring the concentration of melamine, tonyred, clenbuterol or estrogen in food and has the advantages of high detection speed, high accuracy and sensitivity and low cost.

The invention relates to an electrochemical sensor for simultaneously detecting cadmium and lead ions and a preparation method. The sensor is composed of an electrochemical work station, a working electrode, a counter electrode, a reference electrode and an electrolytic tank, wherein the working electrode adopts a glassy carbon electrode as a base, and a Nafion/N-doped micropore carbon composite membrane is modified on the surface of the working electrode; the electrochemical sensor can realize the simultaneous detection of the trace cadmium and lead ions, and the detection limits are respectively 1.5mug/L and 0.05 mug/L. The preparation method of the working electrode provided by the invention has the advantages that simplicity and environmental protection are realized, the working electrode combines the characteristic that hetero atoms and Nafion can effectively adsorb heavy metal ions, and bismuth and to-be-detected heavy metal can form an alloy, and the sensitivity of sensing heavy metal ions of the glassy carbon electrode is remarkably improved. The electrochemical sensor provided by the invention has the advantages that the reproducibility is good, the antijamming capability is strong, the detection limits are low, and the electrochemical sensor is applied in on-site analysis of cadmium and lead ions.

The invention discloses a preparation method for a three-dimensional molybdenum disulfide nanoflower-graphene composite material and application of the three-dimensional molybdenum disulfide nanoflower-graphene composite material as an electrochemical hydrogen evolution catalyst. According to the invention, the three-dimensional molybdenum disulfide nanoflower-graphene composite material is prepared through a one-step hydrothermal method; and the obtained composite material is used to modify a glassy carbon electrode after ultrasonic dispersion so as to obtain a three-dimensional molybdenum disulfide nanoflower-graphene composite material modified electrode. The three-dimensional molybdenum disulfide nanoflower-graphene composite material is mainly applied to electrochemical hydrogen evolution; and a linear scanning curve (polarization curve) is used to detect the catalytic activity of the synthesized molybdenum disulfide nanoflower-graphene composite material, and a cyclic voltammetry curve is employed for testing the stability of the molybdenum disulfide nanoflower-graphene composite material. According to the invention, synergism of molybdenum disulfide nanoflower and graphene in the three-dimensional molybdenum disulfide nanoflower-graphene composite material is made full use of to improve the catalytic efficiency of electrochemical hydrogen evolution and to effectively enhance the stability of the catalyst so as to allow the catalyst to be used in an acidic environment for a long time.

The invention provides a preparation method of a graphene modified glassy carbon electrode and an application thereof. The preparation method comprises the steps of: (1) preparing an oxidized grapheme dispersion liquid; (2) cleaning up the glassy carbon electrode, dropping the oxidized grapheme dispersion liquid on the surface of the glassy carbon electrode and airing; (3) carrying out electrochemical reduction in a buffer solution by using an oxidized graphene modified glassy carbon electrode as a work electrode to obtain the graphene modified glassy carbon electrode; and detecting the work electrode of an oxidoreduction active pigment as the graphene modified glassy carbon electrode. The graphene modified glassy carbon electrode is prepared by electrochemical reduction of the oxidized graphene modified glassy carbon electrode. The method has the advantages of simpleness in operation, short detection time, sensitive response and good analyzing effect.

The invention relates to a manufacturing method of a graphene-porphyrin modified electrode and application of the electrode, aiming to solve the problems of complex operation, long time and relatively high price in the conventional method for detecting the content of ascorbic acid in a living body. The method comprises the following steps: I, preparing pre-oxidized graphene; II, preparing a graphene oxide solid; III, preparing acyl-chlorinated graphene; IV, preparing tetraphenylporphyrin; V, preparing 5-(4-nitro)-10,15,20-triphenyl porphyrin; VI, preparing 5-(4-amino)phenyl-10,15-20-triphenyl porphyrin; VII, preparing a pure manganese porphyrin coordination compound; VIII, preparing a functional composite material of graphene oxide and porphyrin; IX, modifying a glassy carbon electrode. The modified electrode has excellent interference resistance, stability and reproducibility, and can be taken as a biosensor for detecting ascorbic acid in practical samples.

The invention discloses a lead ion specific detection sensor and a preparation method and a using method thereof. The sensor comprises a three-electrode system, wherein a glassy carbon electrode for depositing graphite, chitosan and Au nanoparticles is used as a working electrode of the system, a platinum sheet is used as a counter electrode, and a calomel electrode is used as a reference electrode. When in detection, in the three-electrode system in which the glassy carbon electrode for depositing graphite, chitosan and Au nanoparticles is used as the working electrode, the platinum sheet is used as the counter electrode and the calomel electrode is used as the reference electrode, an electrochemical workstation is used for performing constant-potential lead ion pre-enrichment; and after the enrichment is finished, the lead ion is detected by a differential pulse working mode. Through the detection sensor, simple and quick specific detection on the lead ion is realized; the lead ion with concentration as low as 0.01ug/L can be detected by the method; meanwhile, the lead ion sensor is simple to prepare, has stable performance and can be repeatedly used; and the sample detection time is short, and the operation is convenient.

The invention provides a dopamine polymer/noble metal nanoparticle electrochemical sensor as well as a preparation method and application thereof. The novel bioelectrochemical sensor is a three-electrode system sensor, wherein a reference electrode is a saturated calomel electrode, a counter electrode is a platinum wire electrode, and a working electrode is a glassy-carbon electrode modified by noble metal nanoparticles. According to the invention, noble metal nanoparticles are prepared by dropping a new-made dopamine or a ramification solution thereof and a water solution of a noble metal precursor on the glassy-carbon electrode layer by layer and reacting through a self-assembly method; and a dopamine polymer generated through the reaction has adhesion capacity and can increase the firmness of the noble metal nanoparticles and the glassy-carbon electrode. The dopamine polymer/noble metal nanoparticle electrochemical sensor provided by the invention can be applied to the detection of hydrogen peroxide; and a result indicates that the dopamine polymer/noble metal nanoparticle electrochemical sensor provided by the invention has the advantages of simple and fast preparation method, high firmness, low detection limit, good reproducibility and fast response.

The invention discloses a graphene-copper nanoparticle composite, and preparation and application thereof, and is characterized in that three-dimensional irregular multistage overlapped graphene sheet layers are formed on a grinded glassy carbon electrode or a dry pure titanium piece subjected to the surface treatment, the graphene sheet layers are in different sizes and are overlapped at different directions, and some graphene sheet layers are tilted; copper nanoparticles are densely compounded on the graphene sheet layers. The invention further provides a preparation method of the graphene-copper nanoparticle composite, the method is simple and easy, green and environmental friendly, and the glassy carbon electrode decorated by the graphene-copper nanoparticle composite prepared by the method has good application in the aspect of an electrochemical sensor.

The invention relates to a carbon heterostructure material / beta-cyclodextrin complex modified electrode and a preparation method thereof, belonging to the technical field of electrochemical analysis and detection. The carbon heterostructure material / beta-cyclodextrin complex is loaded on the surface of a glassy carbon electrode and forms a uniform film on the surface of the electrode, the content of carbon heterostructure materials on the surface of the electrode is 3.40-20.80mu g.cm<-2>, and the content of the beta-cyclodextrin on the surface of the electrode is 17.00-79.30mu g.cm<-2>. The carbon heterostructure material of the modified electrode is obtained through hybridization of two-dimensional grapheme and a one-dimensional carbon nanotube and is further compounded with the beta-cyclodextrin with supramolecular properties, an appropriate amount of mixture suspension is dropped on the clean surface of the electrode and is dried naturally, and thus the carbon heterostructure material / beta-cyclodextrin complex modified electrode can be obtained. The invention has the advantages of simple process, low cost, high sensitivity, low detection limit and good repeatability and reproducibility. The carbon heterostructure material / beta-cyclodextrin complex modified electrode can be applied in rapid and high-sensitivity onsite detection in the medical, environmental, food andother fields.

The invention relates to a preparation method of a catalyst material for hydrogen production by virtue of electrolysis of water. The preparation method comprises the steps of soaking graphite carbon nanostructure-graphene composite carbon paper in a precursor solution of molybdenum and sulfur, carrying out a hydrothermal reaction or a solvothermal reaction, and then repeatedly washing to obtain a molybdenum sulfide and graphite carbon nanostructure-graphene composite carbon paper material, namely the catalyst material. Compared with the prior art, the catalyst material can be directly used as a catalytic electrode material for hydrogen production by virtue of electrolysis of water without glassy carbon electrode coating treatment; the preparation method is green and safe, simple in process and low in raw material cost, can realize large-scale continuous production and has wide application prospect in the field of hydrogen production by virtue of electrolysis of water.

The invention discloses a preparation method of a Keggin type heteropoly acid-polypyrrole-graphene composite material modified electrode. The preparation method comprises the following steps: selecting a glassy carbon electrode and carrying out surface treatment on the glassy carbon electrode; dropping graphene dispersion liquid on the surface of the glassy carbon electrode and putting the glassy carbon electrode under an infrared lamp to be dried to prepare a graphene modified electrode; immersing the prepared graphene modified electrode into a sulfuric acid solution containing polypyrrole and Keggin type heteropoly acid; scanning by using a cyclic voltammetry; after scanning, taking out the graphene modified electrode and eluting with water for the second time; drying at room temperature to obtain the Keggin type heteropoly acid-polypyrrole-graphene composite material modified electrode. The Keggin type heteropoly acid-polypyrrole-graphene composite material modified electrode prepared by the preparation method has a sensitive inhibition effect based on an oxidization reduction reaction of folic acid on the Keggin type heteropoly acid and can be used for determining the folic acid; compared with a common electrode, the Keggin type heteropoly acid-polypyrrole-graphene composite material modified electrode has the advantages of small over-potential, high sensitivity, good stability, high reproducibility and the like; a preparation process is simple, raw materials are easy to obtain and the cost is low.

The invention discloses a manufacture method of a silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and the application thereof. The manufacture method of the electrochemical immunosensor includes modifying thionine-graphene mixed solution on the surface of a glassy carbon electrode, conducting cross linking on an antibiotic antibody incubated by Ag-Fe3O4 mesoporous nanometer particles, closing non-specificity active sites by bovine serum albumin to manufacure the antibiotic electrochemical immunosensor. A detection method of antibiotic is that a reference electrode-saturated calomel electrode, an electrode-platinum filament electrode and a working electrode are correctly connected on an electrochemical working station, and immunodetection is conducted through the square wave voltammetry. The antibiotic electrochemical immunosensor has high sensitivity and selectivity, is simple in detection method and has the advantages of being quick, high in efficiency, goodin specificity, low in cost, convenient to operate and the like. It takes to 2-3 minutes to finish one detection process.

The invention discloses a method for preparing a nanometer Co-Fe prussian-blue complex-carbon nano tube composite hydrogen peroxide sensor, which comprises the following steps of: a, preparing Co-Fe prussian-blue complex nano particles; b, performing surface pretreatment on a glassy carbon electrode; c, mixing the Co-Fe prussian-blue complex nano particles and an acidized multi-walled carbon nano tube ultrasonically for 30 to 60 minutes; and d, dropping and coating the ultrasonically-mixed solution in the step c on the glassy carbon electrode, and drying by using an infrared lamp to obtain the novel hydrogen peroxide sensor. In the nanometer Co-Fe prussian-blue complex-carbon nano tube composite hydrogen peroxide sensor prepared by the method, the linear range response to H2O2 is between 1 micrometer and 1.1 millimeters, the detection limit is 0.25 micrometer, and the sensitivity is 255 micro A mM<-1>cm<-2>, so the method is high in sensitivity and low in detection limit.

The invention discloses a preparation method of an ionic liquid modified carbon sphere loaded platinum nanoparticle catalyst, and application of the catalyst to methanol electrocatalysis reaction. The method comprises the following steps of: synthesizing carbon spheres and ionic liquid; modifying the carbon spheres by using the ionic liquid; and performing anion exchange on a platinum precursor and the ionic liquid, and reducing platinum on the surfaces of the ionic liquid modified carbon spheres to obtain the ionic liquid modified carbon sphere loaded platinum nanoparticle catalyst. A catalyst modified glassy carbon electrode is taken as a working electrode, and electrochemical reaction is performed in electrolyte solution of sulfuric acid/methanol. The method can effectively prevent platinum nanoparticles from being agglomerated; and due to the introduction of the ionic liquid, the conductivity of the carbon spheres is further improved, specific surface area is increased, capability of resisting carbon monoxide poisoning can be improved, and the performance of a catalyst carrier is optimized.

The invention discloses a method for applying an ionic liquid functionalized graphene modified electrode in concurrent detection of 5-hydroxytryptamine and dopamine, wherein an amine terminated imidazole ionic liquid and the epoxy group of graphene oxide lamellas are subjected to a nucleophilic ring opening reaction to form ionic liquid functionalized graphene, and then the ionic liquid functionalized graphene is modified on a glassy carbon electrode to form a modification layer so as to concurrently detecting 5-hydroxytryptamine and dopamine. According to the present invention, research results show that the ionic liquid functionalized graphene modified electrode provides good electrocatalysis activity for oxidation of 5-hydroxytryptamine and dopamine, and can be provided for individually detecting 5-hydroxytryptamine and dopamine and concurrently detecting 5-hydroxytryptamine and dopamine, and the method has characteristics of simple detection process, high sensitivity, rapidness and convenience.

The invention discloses a method for quickly detecting lead and cadmium by adopting scanning anodic stripping voltammetry. The method comprises the following steps: a sample to be tested is prepared; lead or cadmium standard liquid is prepared; a three-electrode system consisting of glassy carbon electrodes plated with mercury films, platinum wire counter electrodes and Ag/AgCl reference electrodes is assembled; parameters are set and the scanning anodic stripping voltammetry is adopted to respectively implement parallel determination for current values between working electrodes and reference electrodes of each standard liquid solution until the relative standard deviation of readings is not more than 5%; lead and cadmium heavy metals of the sample are measured through detecting the current values between the working electrodes and the reference electrodes; a working station automatically calculates to obtain detecting results through automatically searching peaks to determine each curve peak area. The detecting method has the advantages of high sensitivity, good accuracy, reduction of mercury pollution, high electrode stability and good reproducibility, and can be applied to the detection of the heavy metals of lead and cadmium with conventional trace and ultratrace.

The invention discloses an electrochemical sensor used for bisphenol A detection, which belongs to the technical field of electrochemical analysis detection. The invention also discloses a preparation method for the electrochemical sensor and an application of detecting bisphenol A in a single pattern. The preparation method for the electrochemical sensor comprises the following steps: grafting beta-cyclodextrin to the surface of a carbon nano tube, wherein the beta-cyclodextrin can be easily dispersed into secondary distilled water; dropwise adding a dispersion to a glassy carbon electrode; and evaporating to dry a solvent to obtain the electrochemical sensor constructed by a sensitive film and the glassy carbon electrode. The electrochemical sensor has high sensitivity on detecting the bisphenol A and high stability, and the detection lower limit reaches 1*10-9mol/L.