65 results about "Thionine" patented technology

The invention relates to an electrochemical immunosensor, and in particular relates to a sandwich type electrochemical immunosensor which is constructed by taking Au-hybridized mesoporous carbon CMK-3(Au@CMK-3) and two electronic mediators, namely neutral red and thionine, as second antibody markers, a preparation method of the sandwich type electrochemical immunosensor, and application of the electrochemical immunosensor, which is prepared by the method, to simultaneous detection on markers of a cervical cancer. The electrochemical immunosensor can be used for simultaneously detecting two samples and overcoming the limitation of a single index, plays an important role in further improvement of the early confirming rate of the cervical cancer, and can be popularized to the field of simultaneous detection on tumor markers of other cancers.

The invention relates to an electrochemical biosensor for organic phosphorus pesticide detection and a preparation method of the electrochemical biosensor. The preparation method comprises the following steps: polymerizing thionine to the surface of a silk-screen printing carbon electrode so as to modify polythionine on the silk-screen printing carbon electrode, fixing acetylcholin esterase and a carboxylation multi-wall carbon nanotube on the surface of the polythionine modified silk-screen printing carbon electrode through chitosan gel, and drying, thereby manufacturing the silk-screen printing carbon electrode, that is, the working electrode of the electrochemical biosensor. The invention provides the electrochemical biosensor which is applied to organic phosphorus pesticide detection and is relatively good in stability, relatively high in sensitivity and relatively low in cost; the invention further provides the preparation method of the electrochemical biosensor for organic phosphorus pesticide detection, the cost of the preparation method is relatively low, and an electrochemical biological bed manufactured by using the electrochemical biosensor is relatively good in stability and relatively high in sensitivity.

The invention relates to a polyoxometallate organic-inorganic composite film with visible light photo-catalytic activity and a preparation method and application thereof. The composite film consists of heteropoly acid anion PW12O40<3-> or SiW12O40<4-> and thionine, and is prepared by a layer-by-layer self-assembly method. The film has good photo-catalytic degradation property to methyl orange coloring matter molecules under the illumination of sunlight, and has potential application prospect in the aspects of environmental protection and energy conservation.

The invention relates to a preparation method of a disulfide dot / nano sheet composite DNA electrochemical probe. The method specifically includes the following steps: 1) ultrasonically stripping the transition metal disulfide powder, high-temperature electromagnetic stirring to prepare a transition metal disulfide quantum dot / nanosheet composite, ultrasonically in a dispersion of thionine and an ionic liquid, Prepare a thionine functionalized complex; 2) Disperse the prepared complex in an organic solvent, make a suspension and drop-coat it on the electrode, and obtain a complex modified electrode after drying; 3) Prepare the above prepared electrode In the three-electrode system, the electrochemical test is performed in the buffer solution, and DNA solutions of different concentrations are injected for testing, and the electrochemical signal changes on the electrode surface are recorded to construct a DNA electrochemical probe system. The probe of the invention has the advantages of high sensitivity, good stability, wide linear range, low detection limit, etc., can be used for detecting DNA in serum, and has broad application prospects in the fields of biological analysis and clinical diagnosis.

The invention discloses an ABA (abscisic acid) concentration detection method based on the biosensing technology. The method can achieve quick detection of the ABA concentration in a to-be-detected organ under the condition that the to-be-detected organ of a to-be-detected plant is not damaged. The method comprises the following steps: S1, integrating a platinum counter electrode, a silver / silver chloride reference electrode and a modified gold working electrode into a microelectrode array, wherein the modified gold working electrode is obtained through sequentially modifying a gold working electrode with thionine, gold nanoparticles and ABA antibody; the platinum counter electrode, the silver / silver chloride reference electrode and the modified gold working electrode are connected with an electrochemical workstation; S2, putting the detection end of the electrode on the microelectrode array into the to-be-detected part of the to-be-detected plant, collecting the impedance generated by the working electrode through using the electrochemical workstation and adopting the alternating current impedance method, calculating the average value of the impedance in preset d uration after the impedance change becomes steady, and substituting the average value into a precomputed linear formula of the ABA concentration and the average value of impedance, so as to obtain the ABA concentration of the to-be-detected part.

The invention discloses an electrode modified with single-walled carbon nanohorn-platinum and palladium nano particles and cytomegalovirus pp65 antibody, and a preparation method and an application thereof. The electrode is prepared by the steps of treating single-walled carbon nanohorn dispersed by a perfluorinated sulfonic acid-polytetrafluoroethylene copolymer membrane, then absorbing thionine, and then combining with the single-walled carbon nanohorn-platinum and palladium nano particles, incubating the cytomegalovirus pp65 antibody and finally sealing with horse radish peroxidase (HRP). The prepared electrode is combined with single-walled carbon nanohorn modified with chitosan; since the single-walled carbon nanohorn has large specific surface area and good biocompatiability, after being modified with amino, the single-walled carbon nanohorn is further beneficial to stabilization of platinum and palladium particles, improvement of the combined quantity of the cytomegalovirus pp65 antibody and increase of the peak current value; in addition, the platinum and palladium particles and HRP can catalyze hydrogen peroxide and amplify a detection signal, thus improving the sensitivity of detection on cytomegalovirus antigen with low concentration.

The invention discloses a method for simultaneously detecting three isomers of benzenediol on basis of a sensor of a thionine functionalized carbon nanotube. The method comprises the following steps of: carrying out chemical reaction on a carboxylated multiwalled carbon nanotube and thionine with amino to form an amidated carbon nanotube, and modifying the amidated carbon nanotube on a glassy carbon electrode to form a modification layer so as to simultaneously detecting the three isomers of the benzenediol. Researches show that by modifying the electrode through the multiwalled carbon nanotube / thionine, the electrochemical response of the isomers of the benzenediol is greatly increased, and a feasible method is provided for simultaneously detecting the three isomers of the benzenediol.

The invention discloses a preparation method and application of a SiO2-MWCNTs enzymatic glucose electrochemical sensor, and belongs to the technical field of electrochemical sensors, wherein the preparation method comprises the steps: taking 0.01-0.05 g of SiO2 nano-powder, fully grinding the SiO2 nano-powder, and adding the ground SiO2 nano-powder into a carbon nanotube NMP dispersion liquid; adding 1 ml of deionized water into the carbon nanotube NMP dispersion liquid, continuously vibrating for 2 days by using an ultrasonic cleaning machine, uniformly mixing the ground SiO2 nano-powder withthe carbon nanotube NMP dispersion liquid, and fully dispersing to obtain a SiO2-MWCNTs composite material; weighing 0.0700 g of thionine and adding into a beaker, and adding deionized water at the temperature of 40 DEG C into the beaker for dissolution; pouring the dissolved thionine solution into a 25 mL brown volumetric flask, and fixing the volume to obtain a 0.01 M thionine solution; and taking 0.001 g of chloroauric acid and putting into a beaker, adding deionized water into the beaker, and carrying out sufficient stirring through a glass rod.

A preparation method and an application of a dye-sensitized heteropolyacid catalyst with visible light photocatalytic activity. The dye-sensitized heteropolyacid catalyst comprises MX12O40<(8-m)-> (M=P, Co, B, Si, As, Ge; X=W, Mo; m is the charge number of the metal ion M), and thionine, and is prepared by a chemical coprecipitation method. The chemical coprecipitation method is simple, and the obtained material is insoluble in water. The material can degrade methyl orange, rose Bengal or organic dyes with a same chromophore as the organic dyes mentioned above in visible light with high efficiency, and has potential application prospects in aspects of environmental protection and energy saving.