65 results about "Thionine" patented technology

The invention discloses a manufacture method of a silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and the application thereof. The manufacture method of the electrochemical immunosensor includes modifying thionine-graphene mixed solution on the surface of a glassy carbon electrode, conducting cross linking on an antibiotic antibody incubated by Ag-Fe3O4 mesoporous nanometer particles, closing non-specificity active sites by bovine serum albumin to manufacure the antibiotic electrochemical immunosensor. A detection method of antibiotic is that a reference electrode-saturated calomel electrode, an electrode-platinum filament electrode and a working electrode are correctly connected on an electrochemical working station, and immunodetection is conducted through the square wave voltammetry. The antibiotic electrochemical immunosensor has high sensitivity and selectivity, is simple in detection method and has the advantages of being quick, high in efficiency, goodin specificity, low in cost, convenient to operate and the like. It takes to 2-3 minutes to finish one detection process.

The invention relates to a preparation method and application of an electrochemical transducer with a signal amplification technology. The preparation method comprises the following steps: firstly, preparing a gold-platinum nano particle, fixing thionine as an electrochemical reagent on the surface of the gold-platinum nano particle, fixing probe DNA on the surface of the gold-platinum nano particle so as to obtain an electrochemical probe, and simultaneously, self-assembling a hairpin DNA on the surface of a gold electrode to obtain an electrochemical transducer. Target DNA and the hairpin DNA assembled on the surface of the gold electrode are partially complemented and paired so as to open a hairpin structure when the target DNA is added to the electrochemical transducer, then another hairpin DNA chain replaces a target DNA chain through a competitive effect, and thus the target DNA chain is released; the released target chain unceasingly opens other hairpin structured DNA of the surface of an electrode, thereby realizing the recycling of the target DNA chain under the cyclic action, and the recycling action is combined the prepared electrochemical probe to measure target DNA. The transducer provided by the invention is high in selectivity and detection sensitivity, the gold-platinum nano particle is utilized as a carrier to support thionine to form the probe, and the transducer has the advantage of electrochemical stability.

The invention relates to an electrochemical immunosensor, and in particular relates to a sandwich type electrochemical immunosensor which is constructed by taking Au-hybridized mesoporous carbon CMK-3(Au@CMK-3) and two electronic mediators, namely neutral red and thionine, as second antibody markers, a preparation method of the sandwich type electrochemical immunosensor, and application of the electrochemical immunosensor, which is prepared by the method, to simultaneous detection on markers of a cervical cancer. The electrochemical immunosensor can be used for simultaneously detecting two samples and overcoming the limitation of a single index, plays an important role in further improvement of the early confirming rate of the cervical cancer, and can be popularized to the field of simultaneous detection on tumor markers of other cancers.

The invention provides an electrochemical detection method for detecting kanamycin residues based on a nucleic acid aptamer and a nano analogue enzyme and belongs to the technical field of analytical chemistry. Gold nano-particles are synthesized by reducing chloroauric acid through tyrosine, and hydrogen peroxide and reduced-state thionine are catalyzed to react to generate oxidized-state thionine; and the thionine can be detected through a difference pulse voltammetry. The gold nano-particles are modified through utilizing a kanamycin specific aptamer; and the aptamer is adsorbed on the surfaces of the gold nano-particles so that the peroxidase activity is inhibited. When a target object kanamycin exists, the aptamer can be competitively replaced from the surfaces of the gold nano-particles to form a compound, so that the peroxidase activity is recovered. The detection of the kanamycin can be realized through detecting a relation between a reduction peak current value of the oxidized-state thionine and an antibiotic concentration by utilizing the difference pulse voltammetry. The method provided by the invention has good repeatability, good stability and high sensitivity, and can be used for effectively detecting kanamycin residues in food samples.

The invention discloses a preparation method of an aptamer sensor for detecting antibiotic residue, belonging to the technical field of safety test of agricultural products. The method comprises the steps of carrying out electrochemical polymerization on a gold electrode and thionine to obtain electrically polymerized thionine electrode; after that, evenly dripping the prepared carbon nano tube-graphene nano-composition onto the surface of the electrically polymerized thionine electrode; drying in the air, and then soaking the electrode into antibiotic aptamer solution; washing, drying in the air, and closing by bull serum albumin (BSA) solution to obtain the antibiotic aptamer sensor. The prepared aptamer sensor has the advantages of not needing pretreatment, being high in analysis speed, simple in an operation system, high in accuracy, high in specificity, low in cost, rapid in response and the like, and has the limit of detection reaching up to 0.8nm, thus being suitable for rapidly detecting the antibiotic residue.

The invention discloses a method used for detecting alpha fetoprotein based on graphene, thionine, and a nucleic acid aptamer. According to the method, electrodeposition technology is adopted for deposition of nano-gold on the surface of a screen-printed electrode, electrostatic adsorption is adopted for absorption of graphene and thionine which drop on an electrode surface onto the surface of thescreen-printed electrode, thionine is taken as a bridge molecule for capturing of the nucleic acid aptamer onto the surface of a modified electrode. A nanometer aptamer biosensor can be constructed based on the extremely large specific surface area and signal amplification effect of graphene, the high loading capacity of thionine on the aptamer, and the specific recognition effect of the aptameron alpha fetoprotein. Peak value current is measured through differential pulse voltammetry, and the relation curve of the current to the alpha fetoprotein concentration is drawn, so that detection onalpha fetoprotein is short in time, low in cost, and high in the specificity.

The invention relates to the technical field of electrochemical analysis and measurement, and in particular relates to a method for detecting the anthracene-phenanthrene resultant; according to the method, thionine is as a probe and is mixed with graphene to prepare an electrochemical modified electrode, and then the electrochemical modified electrode is used for quantitative detection of anthracene-phenanthrene resultant. The functional modified electrode in which a mesoporous material is modified by using the probe has the advantages that the material synthesis is simple and convenient, the modified electrode is relatively simple to prepare, the material is low in price, the electrode is easy to update, good in reproducibility and free of toxicity and does not cause environment pollution, and moreover by utilizing the detection method, the interference of coexisting substances is effectively reduced; and selectivity and sensation are high.

The invention relates to an electrochemical biosensor for organic phosphorus pesticide detection and a preparation method of the electrochemical biosensor. The preparation method comprises the following steps: polymerizing thionine to the surface of a silk-screen printing carbon electrode so as to modify polythionine on the silk-screen printing carbon electrode, fixing acetylcholin esterase and a carboxylation multi-wall carbon nanotube on the surface of the polythionine modified silk-screen printing carbon electrode through chitosan gel, and drying, thereby manufacturing the silk-screen printing carbon electrode, that is, the working electrode of the electrochemical biosensor. The invention provides the electrochemical biosensor which is applied to organic phosphorus pesticide detection and is relatively good in stability, relatively high in sensitivity and relatively low in cost; the invention further provides the preparation method of the electrochemical biosensor for organic phosphorus pesticide detection, the cost of the preparation method is relatively low, and an electrochemical biological bed manufactured by using the electrochemical biosensor is relatively good in stability and relatively high in sensitivity.

The invention discloses an electrochemical sensor for detecting telomerase activity and a method for manufacturing the electrochemical sensor. The method for manufacturing the electrochemical sensor mainly includes procedures of sequentially adsorbing DNA (deoxyribonucleic acid) strands S1 of thionine, Au@SiO<2> and G-rich structures in the surfaces of molybdenum disulfide nano-sheets and adding Hemin into the molybdenum disulfide nano-sheets to form MoS<2>-Thi-Au@Si<O>2 / DNAzyme nano-composite materials; modifying TS (telomerase substrate) precursors on the surfaces of gold electrodes, extending the DNA strands by the aid of telomerase to form DAN strands S2, screwing the DNA strands S2 and the DNA strands S1 complementarily, and ultimately detecting the intensity of electrochemical signals of catalytic hydrogen peroxide oxidation ABTS to directly detect the telomerase activity. The electrochemical sensor and the method have the advantages that the electrochemical sensor manufactured by the aid of the method is simple in operation and high in sensitivity and stability and has an important significance on detecting cancerous cells.

The invention relates to a method and application for electrochemically determining dopamine on the basis of an aptamer recognition effect. The method is characterized by comprising the following steps: adsorbing electrochemical marker thionine onto the surface of gold-platinum (Au@PtNPs) nano particles, fixing sulfydryl DNA1 on the surface of Au@PtNPs to obtain an electrochemical probe (DNA1 / Th / Au@PtNPs); adding dopamine aptamer chain DNA2 into an electrochemical probe solution so as to facilitate the supplementary pairing of the DNA1 and the DNA2 to obtain electrochemical probe (DNA2 / DNA1 / Th / Au@PtNPs) modified by the DNA2, wherein after the dopamine is added into the probe solution modified by the DNA2, the aptamer chain DNA2 is combined with the dopamine, so that the electrochemical probe DNA1 / Th / Au@PtNPs is regenerated; immersing a carbon nano-particle-modified gold electrode (CNPs / GE) into the regenerated electrochemical probe DNA1 / Th / AuPtNPs solution, wherein due to the adsorption effect of the carbon nano particles and the single-chain DNA1, the electrochemical probe is adsorbed onto the surface of the electrode, the electrochemical signal is detected, and the quantitative determination of dopamine can be realized by the electrochemical method through the intensity of the electrochemical signal. The sensor is high in selectivity and detection sensitivity.

The invention discloses a method for simultaneously detecting zearalenone (ZEN) and fumonisin B1 (FB1), and also discloses an aptamer sensor for simultaneously detecting the zearalenone and the fumonisin. The aptamer sensor is prepared by the following method: (1) covering a glassy carbon electrode with reducing molybdenum disulfide and a gold nanoparticle composite (rMoS2-Au) nanomaterial, and drying; (2) subsequently dripping a mixed solution of a zearalenone nucleic acid aptamer (AP1) and a fumonisin B1 nucleic acid aptamer (AP2) onto the electrode, incubating for 1-10h at 1-10 mu mol / L, v / v, 1 / 1, and blocking the same with 1 mg / mL QBSA; and (3) finally, dripping a mixed solution of a self-developed ZEN nucleic acid aptamer partial complementary sequence (CP1-Au-Thi) solution of 5'-endmodified gold nanoparticles and thionine and a FB1 nucleic acid aptamer partial complementary sequence (CP2-Au-FC6S) solution of 5'-end modified gold nanoparticles and ferrocenyl hexanethiol, and incubating for 1-10h at 1-10 mu mol / L, v / v, 1 / 1 to obtain the required aptamer sensor. The method provided by the invention is suitable for the rapid and simultaneous detection of QEN and FB1 in agricultural products such as corn and feed, thereby effectively preventing the agricultural products with over-standard QEN and FB1 from flowing into the market, and the diet safety of people is guaranteed asa result.

The invention discloses a method for simultaneously detecting three isomers of benzenediol on basis of a sensor of a thionine functionalized carbon nanotube. The method comprises the following steps of: carrying out chemical reaction on a carboxylated multiwalled carbon nanotube and thionine with amino to form an amidated carbon nanotube, and modifying the amidated carbon nanotube on a glassy carbon electrode to form a modification layer so as to simultaneously detecting the three isomers of the benzenediol. Researches show that by modifying the electrode through the multiwalled carbon nanotube / thionine, the electrochemical response of the isomers of the benzenediol is greatly increased, and a feasible method is provided for simultaneously detecting the three isomers of the benzenediol.

The invention belongs to the technical field of biosensors, and particularly relates to a preparation method of an unmarked ratio electrochemical sensor for detecting aflatoxin B1 based on time regulation sensitivity. The invention discloses a label-free signal probe-thionine and reduced graphene oxide (THI-rGO) composite material. The composite material is formed through non-covalent interactionof thionine (THI) and reduced graphene oxide (rGO). The gold nanoparticles (AuNPs) fix sulfydryl modified aptamer complementary chains on a sensing interface through Au-S covalent bonds; and the aptamers marked by the electrochemical active molecules Fc at the 3'and 5' ends are fixed on a modified sensing interface through the complementary pairing effect of basic groups, so that the novel sensoris constructed. Furthermore, the ratio (ITHI / IFc) of ratio signals is regulated and controlled by regulating and controlling the cDNA action time of the Fc-modified AFB1 aptamer and a sensing interface, so that the novel sensitivity-controllable label-free ratio electrochemical biosensor is obtained and is used for sensitively and quickly analyzing an actual sample AFB1.

The invention relates to a polyoxometallate organic-inorganic composite film with visible light photo-catalytic activity and a preparation method and application thereof. The composite film consists of heteropoly acid anion PW12O40<3-> or SiW12O40<4-> and thionine, and is prepared by a layer-by-layer self-assembly method. The film has good photo-catalytic degradation property to methyl orange coloring matter molecules under the illumination of sunlight, and has potential application prospect in the aspects of environmental protection and energy conservation.

The invention relates to a streptavidin functionalized semiconductor nano material-based tumor marker electrochemical immunosensor and a preparation method thereof. The immunosensor is prepared by the following steps: firstly, synthesizing or screening the semiconductor nano structure material with excellent performance, carrying out bio-functionalization by using the streptavidin, and then specifically combining with a biotinylation antibody. The prepared sensor is incubated in a mixed solution containing an enzyme-labeled antibody and an antigen, thionine and hydrogen peroxide are taken as enzyme reaction substrates, and the tumor marker is detected by using a sandwich immunoassay method. An ampere response of an electrode is increased along with an increase of antigen concentration in an incubation solution. The detection method is high in sensitivity, low in detection limit, and good in reproducibility and stability, and can be applied to quantitative determination of the tumor marker in serum.

The invention discloses a preparation method of CdSe / ZnS core-shell quantum dots and an application of the CdSe / ZnS core-shell quantum dots in detecting trace explosive 4-nitrotoluene, and belongs to the technical fields of analytic chemistry and nanometer. The CdSe / ZnS core-shell quantum dots are produced from cadmium perchlorate, selenium powder, aluminum powder, methionine, thiohydracrylic acid, cadmium chloride, thioacetamide and zinc chloride; and the quantum dots are used for detecting the explosive 4-nitrotoluene. The method has high sensitivity, and is capable of detecting the 4-nitrotoluene low to 2.4*10<-9>mol / L.

A method for using thiazine dyes, especially methylene blue, alone or in combination with low levels of light, to treat or prevent avian influenza virus is described. Examples of useful thiazine dyes are methylene blue, azure A, azure C, toluidine, and thionine. The preferred dye is methylene blue, administered orally twice a day.

The invention, which belongs to the technical field of immunoassay and biosensing, provides a preparation method and application of a nitrogen-sulfur-double-doped-graphene-oxide-labeled sandwich typeimmunosensor. Thionine and gold platinum nuclear-shell dendritic crystal nanoparticle functionalized nitrogen-sulfur double-doped graphene oxide is used a catalytic material; and after incubation of the thionine and gold platinum nuclear-shell dendritic crystal nanoparticle functionalized nitrogen-sulfur double-doped graphene oxide with a detection antibody, a labeling substance is formed. Gold nano rod functionalized polydopamine is used as an electrode modification material. The prepared sandwich type electrochemical immunosensor is capable of realizing quantitative detection of cardiac troponin cTnI and cTnT by differential pulse voltammetry. The sensitivity and specificity are high and the detection limit is low. The preparation method and application of a nitrogen-sulfur-double-doped-graphene-oxide-labeled sandwich type immunosensor has the important scientific significance and great application value in early-stage detection of the cardiac troponin.

The present invention relates to a substrate-free electrochemical biosensor for detecting uracil-DNA glycosylase based on non-enzymatic nano-material signal amplification. The non-enzymatic nano material (OAPS-Por) used in the sensor not only can adsorb a large amount of thionine molecules serving as an electroactive substance, but also can catalyze reduction of thionine on an electrode under thecondition that H2O2 does not participate, so that the signal intensity is greatly enhanced. On the basis of the material, a signal probe (OAPS-Por / Thi@AuNps-ssDNA) is prepared. In the presence of UDG,uracil bases in hairpin DNA on the electrode are removed, a hairpin structure is expanded, and the signal probe is connected to the surface of the electrode through DNA base complementary pairing, sothat an amplified thionine reduction peak is generated, and the constructed biosensor shows a relatively wide linear range (0.005-1U / ml), and the detection limit is 0.000697U / ml. In addition, the biosensor can also be used for detecting the activity of UDG in a UDG activity inhibitor and a HeLa cell lysis solution, and has huge potential in clinical diagnosis and biomedical research.

The invention relates to a preparation method of a disulfide dot / nano sheet composite DNA electrochemical probe. The method specifically includes the following steps: 1) ultrasonically stripping the transition metal disulfide powder, high-temperature electromagnetic stirring to prepare a transition metal disulfide quantum dot / nanosheet composite, ultrasonically in a dispersion of thionine and an ionic liquid, Prepare a thionine functionalized complex; 2) Disperse the prepared complex in an organic solvent, make a suspension and drop-coat it on the electrode, and obtain a complex modified electrode after drying; 3) Prepare the above prepared electrode In the three-electrode system, the electrochemical test is performed in the buffer solution, and DNA solutions of different concentrations are injected for testing, and the electrochemical signal changes on the electrode surface are recorded to construct a DNA electrochemical probe system. The probe of the invention has the advantages of high sensitivity, good stability, wide linear range, low detection limit, etc., can be used for detecting DNA in serum, and has broad application prospects in the fields of biological analysis and clinical diagnosis.

The invention discloses an ABA (abscisic acid) concentration detection method based on the biosensing technology. The method can achieve quick detection of the ABA concentration in a to-be-detected organ under the condition that the to-be-detected organ of a to-be-detected plant is not damaged. The method comprises the following steps: S1, integrating a platinum counter electrode, a silver / silver chloride reference electrode and a modified gold working electrode into a microelectrode array, wherein the modified gold working electrode is obtained through sequentially modifying a gold working electrode with thionine, gold nanoparticles and ABA antibody; the platinum counter electrode, the silver / silver chloride reference electrode and the modified gold working electrode are connected with an electrochemical workstation; S2, putting the detection end of the electrode on the microelectrode array into the to-be-detected part of the to-be-detected plant, collecting the impedance generated by the working electrode through using the electrochemical workstation and adopting the alternating current impedance method, calculating the average value of the impedance in preset d uration after the impedance change becomes steady, and substituting the average value into a precomputed linear formula of the ABA concentration and the average value of impedance, so as to obtain the ABA concentration of the to-be-detected part.

The invention discloses an electrode modified with single-walled carbon nanohorn-platinum and palladium nano particles and cytomegalovirus pp65 antibody, and a preparation method and an application thereof. The electrode is prepared by the steps of treating single-walled carbon nanohorn dispersed by a perfluorinated sulfonic acid-polytetrafluoroethylene copolymer membrane, then absorbing thionine, and then combining with the single-walled carbon nanohorn-platinum and palladium nano particles, incubating the cytomegalovirus pp65 antibody and finally sealing with horse radish peroxidase (HRP). The prepared electrode is combined with single-walled carbon nanohorn modified with chitosan; since the single-walled carbon nanohorn has large specific surface area and good biocompatiability, after being modified with amino, the single-walled carbon nanohorn is further beneficial to stabilization of platinum and palladium particles, improvement of the combined quantity of the cytomegalovirus pp65 antibody and increase of the peak current value; in addition, the platinum and palladium particles and HRP can catalyze hydrogen peroxide and amplify a detection signal, thus improving the sensitivity of detection on cytomegalovirus antigen with low concentration.

The invention discloses a double signal-double recognition imprinted electrochemical sensor and a preparation method thereof and a detection method of ovalbumin. The preparation method includes the steps that (1), electrochemical polymerization is conducted on the surface of an electrode carrier to form a poly-p-aminobenzene sulfonic acid film pABSA to obtain a pABSA / electrode carrier; (2), electrochemical polymerization is conducted on the surface of the pABSA / electrode carrier to form a poly thionine film pTH to obtain a pTH / pABSA / electrode carrier; and (3), the pTH / pABSA / electrode carrier is put into a mixed solution for electrochemical polymerization, and then the electrode is eluted to remove ovalbumin OVA to form an imprinted polymer MIP to obtain a MIP / pTH / pABSA / electrode carrier, wherein, the mixed solution contains ovalbumin OVA and 3-thienylboric acid 3-TBA. The double signal-double recognition imprinted electrochemical sensor has excellent sensitivity and selectivity for thedetection of the ovalbumin.

The invention belongs to the field of biological, and particularly relates to a method for detecting trypsinase activity. A simple and convenient method for detecting the trypsinase activity is builtby using the mutual action differences of polypeptides before and after the trypsinase enzyme digestion and graphene oxide loaded with electrochemical active molecule thionine. The graphene oxide haslarger specific surface area, and can load a great amount of thionine; the effects of amplifying electrochemical signals and improving the detection sensitivity are achieved. The gold electrode surface is modified by gold sulfhydryl bond covalent formed by gold and sulfhydryl of tail end cysteine via substrate polypeptides; the sulfhydryl-containing polypeptides are subjected to covalent modification onto the gold surface; then, 6-mercapto-l-hexanol is used for space occupation; non-specific adsorption polypeptides are removed; the electrode surface blank position is sealed; the N tail end ofthe substrate polypeptide sequence is provided with histidine; the histidine has positive charges; graphene oxide containing rich carboxyls have negative charges after dissociation; stronger static electricity mutual action exists between the histidine and the graphene oxide, so that a prepared compound is adsorbed to the final modified electrode product.

The invention discloses a method for simultaneously detecting three isomers of benzenediol on basis of a sensor of a thionine functionalized carbon nanotube. The method comprises the following steps of: carrying out chemical reaction on a carboxylated multiwalled carbon nanotube and thionine with amino to form an amidated carbon nanotube, and modifying the amidated carbon nanotube on a glassy carbon electrode to form a modification layer so as to simultaneously detecting the three isomers of the benzenediol. Researches show that by modifying the electrode through the multiwalled carbon nanotube / thionine, the electrochemical response of the isomers of the benzenediol is greatly increased, and a feasible method is provided for simultaneously detecting the three isomers of the benzenediol.

The invention discloses a preparation method and application of a SiO2-MWCNTs enzymatic glucose electrochemical sensor, and belongs to the technical field of electrochemical sensors, wherein the preparation method comprises the steps: taking 0.01-0.05 g of SiO2 nano-powder, fully grinding the SiO2 nano-powder, and adding the ground SiO2 nano-powder into a carbon nanotube NMP dispersion liquid; adding 1 ml of deionized water into the carbon nanotube NMP dispersion liquid, continuously vibrating for 2 days by using an ultrasonic cleaning machine, uniformly mixing the ground SiO2 nano-powder withthe carbon nanotube NMP dispersion liquid, and fully dispersing to obtain a SiO2-MWCNTs composite material; weighing 0.0700 g of thionine and adding into a beaker, and adding deionized water at the temperature of 40 DEG C into the beaker for dissolution; pouring the dissolved thionine solution into a 25 mL brown volumetric flask, and fixing the volume to obtain a 0.01 M thionine solution; and taking 0.001 g of chloroauric acid and putting into a beaker, adding deionized water into the beaker, and carrying out sufficient stirring through a glass rod.

The invention relates to a preparation method of an okadaic acid three-dimensional gold nanopillar array immunity electrode. The preparation method comprises the following steps: depositing gold on apolycarbonate filter membrane with the aperture being 80-200 nm with a chemical deposition-electro-deposition method, so as to obtain a three-dimensional gold nanopillar array electrode; electro-polymerizing and decorating poly(thionine) on the surface of the three-dimensional gold nanopillar array electrode with a cyclic voltammetry, so as to form a poly(thionine) / three-dimensional gold nanopillar array electrode; binding glutaraldehyde on the poly(thionine) / three-dimensional gold nanopillar array electrode and fixing an okadaic acidantibody on the glutaraldehyde, so as to form an okadaic acidantibody / glutaraldehyde / poly(thionine) / three-dimensional gold nanopillar array electrode; and finally, closing the electrode with bovine serum albumin, so as to obtain the okadaic acid three-dimensional gold nanopillar array immunity electrode. The electrode provided by the invention has a three-dimensional structure and is large in superficial area, simple in preparation, high in stability, firmin fixation of the antibody, simple and convenient in operation, low in detection limit and high in sensibility, and rapid detection can be achieved.

A preparation method and an application of a dye-sensitized heteropolyacid catalyst with visible light photocatalytic activity. The dye-sensitized heteropolyacid catalyst comprises MX12O40<(8-m)-> (M=P, Co, B, Si, As, Ge; X=W, Mo; m is the charge number of the metal ion M), and thionine, and is prepared by a chemical coprecipitation method. The chemical coprecipitation method is simple, and the obtained material is insoluble in water. The material can degrade methyl orange, rose Bengal or organic dyes with a same chromophore as the organic dyes mentioned above in visible light with high efficiency, and has potential application prospects in aspects of environmental protection and energy saving.

The invention relates to the technical field of micro electrode biology, in particular to a micro rate type sensor for bio-assay of salicyclic acid as well as a construction method and application thereof. An electrochemical sensor generally outputs a single signal of one substance. The invention provides an SA micro rate type sensor capable of outputting two signals, so that the SA micro rate type sensor can be better applied to the viviperception of plants. An electrode is modified by adopting a nano cluster of thionine / GO / MWNT, the thionine is used as a built-in correction signal and formsa dual-signal output together with an SA signal, thereby constructing the rate type sensor, significantly improving the stability and reproducibility of the SA sensor, and improving the accuracy and reliability when in plant in-vivo SA detection.