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Preparation method of okadaic acid three-dimensional gold nanopillar array immunity electrode

A gold nanocolumn and array electrode technology, which is applied in the field of immune electrode preparation, can solve the problems of poor stability of three-dimensional gold nanocolumn array electrodes, affect the accuracy of immune sensors, and large differences in etching effects, etc., to achieve electrode stability Good, lower detection cost, high sensitivity

Active Publication Date: 2018-11-20
HARBIN INST OF TECH AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are certain defects in the etching method, the error of human operation is greatly affected, and the etching effect is quite different, which leads to poor stability of the prepared three-dimensional gold nanopillar array electrode, which affects the accuracy of immunosensor detection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: A kind of preparation method of okadaic acid three-dimensional gold nanocolumn array immune electrode, it comprises the following steps:

[0017] (1) Preparation of three-dimensional gold nanopillar array electrodes:

[0018] a) First fix the polycarbonate filter membrane with a pore size of 80-200 nm, wash it in methanol for 5 minutes, and then wash it in methanol-water solution (methanol:water volume ratio = 1:1);

[0019] b) Put the cleaned polycarbonate filter membrane into the sensitization solution for sensitization treatment. The components of the sensitization solution used are: 0.025~0.028 mol / L SnCl 2 , 0.05~0.08 mol / L CF 3 COOH, the solvent is methanol-water solution with a volume ratio of 1:1, and the sensitization time is 40~50 min;

[0020] c) Put the polycarbonate filter membrane after sensitization and cleaning into 0.03 mol / L silver ammonia solution for activation and pre-silvering, and the activation time is 5 minutes;

[0021] d) Put ...

Embodiment 2

[0030] Embodiment 2: A kind of preparation method of okadaic acid three-dimensional gold nanocolumn array immune electrode, it comprises the following steps:

[0031] (1) Preparation of three-dimensional gold nanopillar array electrodes:

[0032] a) First fix the polycarbonate filter membrane with a pore size of 80-200 nm, wash it in methanol for 5 minutes, and then wash it in methanol-water solution (methanol:water volume ratio = 1:1);

[0033] b) Put the cleaned polycarbonate filter membrane into the sensitization solution for sensitization treatment. The components of the sensitization solution used are: 0.025~0.028 mol / L SnCl 2 , 0.05~0.08 mol / L CF 3 COOH, the solvent is methanol-water solution with a volume ratio of 1:1, and the sensitization time is 40~50 min;

[0034] c) Put the polycarbonate filter membrane after sensitization and cleaning into 0.03 mol / L silver ammonia solution for activation and pre-silvering, and the activation time is 5 minutes;

[0035] d) Put ...

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Abstract

The invention relates to a preparation method of an okadaic acid three-dimensional gold nanopillar array immunity electrode. The preparation method comprises the following steps: depositing gold on apolycarbonate filter membrane with the aperture being 80-200 nm with a chemical deposition-electro-deposition method, so as to obtain a three-dimensional gold nanopillar array electrode; electro-polymerizing and decorating poly(thionine) on the surface of the three-dimensional gold nanopillar array electrode with a cyclic voltammetry, so as to form a poly(thionine) / three-dimensional gold nanopillar array electrode; binding glutaraldehyde on the poly(thionine) / three-dimensional gold nanopillar array electrode and fixing an okadaic acidantibody on the glutaraldehyde, so as to form an okadaic acidantibody / glutaraldehyde / poly(thionine) / three-dimensional gold nanopillar array electrode; and finally, closing the electrode with bovine serum albumin, so as to obtain the okadaic acid three-dimensional gold nanopillar array immunity electrode. The electrode provided by the invention has a three-dimensional structure and is large in superficial area, simple in preparation, high in stability, firmin fixation of the antibody, simple and convenient in operation, low in detection limit and high in sensibility, and rapid detection can be achieved.

Description

technical field [0001] The invention relates to a preparation method of an immune electrode, in particular to a preparation method of an okadaic acid three-dimensional gold nano-column array immune electrode. Background technique [0002] Okadaic acid (Okadaic Acid, OA) is a type of diarrheal shellfish toxin in marine toxins. When okadaic acid ingested in the human body reaches a certain level, it will cause symptoms such as diarrhea, nausea, vomiting and abdominal pain. At the same time, long-term accumulation of okadaic acid in the body can also induce tumors and carcinogenicity, which is a great hazard to human health and safety. The maximum allowable amount of okadaic acid stipulated by the EU is 160 ng / g. Rapid, simple and accurate detection of okadaic acid content is of great significance to the safety of human life. The traditional detection methods of okadaic acid mainly include: mouse test method, high performance liquid chromatography, liquid chromatography-tande...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N27/327
CPCG01N27/327G01N33/531
Inventor 曹立新梁晨希孙帅刘海萍毕四富
Owner HARBIN INST OF TECH AT WEIHAI
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