2051results about How to "Short analysis time" patented technology

A method for removing a target DNA fragment having a predetermined base-pair length from a mixture of DNA fragments comprises the following steps. A mixture of DNA fragments which may contain the target DNA fragments is applied to a separation column containing media having a nonpolar, nonporous surface, the mixture of DNA fragments being in a first solvent mixture containing a counterion and a DNA binding concentration of driving solvent in a cosolvent. The target DNA fragments are separated from the media by contacting it with a second solvent solution containing a counterion and a concentration of driving solvent in cosolvent which has been predetermined to remove DNA fragments having the target DNA fragment base pair length from the media. The target DNA fragments can be collected and optionally amplified. When the method is being applied to collect a putative fragment, if present, no DNA fragments having the base pair length of the target DNA could be present in the mixture. Alternatively, DNA fragments having the base pair length of the target DNA are present in the mixture. The disclosure also describes an ambient or low pressure device for separating polynucleotide fragments from a mixture of polynucleotide fragments comprises a tube having an upper solution input chamber, a lower eluant receiving chamber, and a fixed unit of separation media supported therein. The separation media has nonpolar separation surfaces which are free from multivalent cations which would react with counterion to form an insoluble polar coating on the surface of the separation media.

An image processing apparatus includes a face detector detecting face images from still-image frames successively extracted from a moving-image stream in accordance with image information items regarding the still-image frames, a face-feature-value calculation unit calculating face feature values of the face images in accordance with image information items regarding the face images, an identity determination unit determining whether a first face image in a current frame and a second face image in a previous frame represent an identical person in accordance with at least face feature values of the first and second face images, and a merging processor which stores one of the first and second face images when the first face image and the second face image represent an identical person, and which stores the first and second face images when the first face image and the second face image do not represent an identical person.

Inventive methods and apparatus are useful for collecting magnetic materials in one or more magnetic fields and resuspending the particles into a dispersion medium, and optionally repeating collection / resuspension one or more times in the same or a different medium, by controlling the direction and rate of fluid flow through a fluid flow path. The methods provide for contacting derivatized particles with test samples and reagents, removal of excess reagent, washing of magnetic material, and resuspension for analysis, among other uses. The methods are applicable to a wide variety of chemical and biological materials that are susceptible to magnetic labeling, including, for example, cells, viruses, oligonucleotides, proteins, hormones, receptor-ligand complexes, environmental contaminants and the like.

Inventive methods and apparatus are useful for collecting magnetic materials in one or more magnetic fields and resuspending the particles into a dispersion medium, and optionally repeating collection / resuspension one or more times in the same or a different medium, by controlling the direction and rate of fluid flow through a fluid flow path. The methods provide for contacting derivatized particles with test samples and reagents, removal of excess reagent, washing of magnetic material, and resuspension for analysis, among other uses. The methods are applicable to a wide variety of chemical and biological materials that are susceptible to magnetic labeling, including, for example, cells, viruses, oligonucleotides, proteins, hormones, receptor-ligand complexes, environmental contaminants and the like.

The invention relates to a preparation method of covalent organic framework composite microspheres with core-shell structures. The method comprises the following steps: dissolving silicon dioxide microspheres, a first construction element and a second construction element in an organic solvent; and after adding a catalyst, rapidly synthesizing into the composite microspheres with core-shell structures at a certain temperature. In a preparation process, reaction conditions are gentle, the method is simple, and the yield is high; the prepared covalent organic framework composite microspheres have the advantages of good core-shell morphology, high specific surface area, ordered pore structures, good mechanical stability, thermal stability, chemical stability and the like, if the microspheres are used as a chromatographic stationary phase, the chromatographic mass transfer resistance can be reduced effectively, the theoretical plate height is improved, and finally, column efficiency and degree of separation are improved; and if the microspheres are used as solid-phase extracting filler, the enrichment effect can be improved remarkably. The covalent organic framework composite microspheres with core-shell structures have good application prospects in the aspect of separation and enrichment of small organic molecules.

The invention discloses application of glutathione-based stable gold nano cluster particles to detection of a sulfhydryl compound, wherein the glutathione-based stable gold nano cluster particles are prepared from a chloroauric acid solution and a glutathione solution, wherein a molar ratio of chloroauric acid to glutathione is between 1:0.5 and 1:1. By using an fluorescence enhancement characteristic of the glutathione-based stable gold nano cluster particles after the addition of the sulfhydryl compound, through detecting the change of fluorescence intensity before and after the sulfhydryl compound with an unknown concentration reacts with the glutathione-based stable gold nano cluster particles, a linear standard curve chart is drawn, and a calibration relation is obtained by adopting linear fitting, so that the sulfhydryl compound with the unknown concentration is detected.

The invention provides a method for identifying viral files, which comprises: firstly, establishing a virtual system, operating a suspicious file in the virtual system, recording behavior information of the suspicious file, judging whether the suspicious file is the viral file or not according to the behavior information and a virus behavior feature library, marking the suspicious file to be the viral file if the suspicious file is the viral file, and otherwise marking the suspicious file to be a safe file. The invention also provides a device for identifying the viral files, which comprises a virtual system module which is used to establish the virtual system, to orient behaviors of the suspicious file to the virtual system, and to operate the suspicious file, a behavior information collecting module which is used to record the behavior information of the suspicious file, and a behavior characteristic analyzing module which is used to mark the suspicious file to be virus when the suspicious file is judged to be the virus according to the behavior information and the virus behavior feature library and is used to mark the suspicious file to be the safe file when the suspicious file is judged to be the safe file.

The invention discloses a vibration analysis method for dry frictional damping mistuned blades with tendons and shroud bands. The method comprises the steps of for actual mistuned states of the blades of a steam turbine, performing three-dimensional contact finite element analysis firstly to obtain support reactions of nodes of contact surfaces of the tendons and the shroud bands of the entire circle of the blades; secondly establishing a contact surface relationship by adopting spring damping units, considering positive pressures, areas and frictional coefficients at the spring damping units, and building a dry frictional damping mistuned vibration analysis model based on a Mindlin micro-sliding friction model and a harmonic balance method; thirdly considering a pre-stress and adopting harmonic response analysis for obtaining convergency values of vibration responses of the blades through iterative solving; and through cyclic calculation, obtaining vibration amplitude response curves of the dry frictional mistuned blades, and performing further extraction to obtain an inherent frequency and a vibration mode. The method is of important significance for dry frictional damping mistuned analysis of the blades and improvement of mistuned bladed disk vibration.

The invention relates to a transformer short-circuit fault comprehensive diagnosis method. The method is technically characterized by including the steps of checking a device once after the short-circuit fault happens to a transformer, analyzing the running working conditions and the protection action conditions to obtain the fault current, the working time and short-circuit process parameters, conducting conventional electrical experimental analysis and oil chromatogram experimental analysis so that whether faults or defects already exist in the transformer or not can be analyzed and the corresponding degrees and positions can be analyzed, conducting the winding deformation test so that whether deformation or concave-convex deformation happens to windings or not can be judged, demonstrating the short-circuit resistant capacity, and comprehensively judging the short-circuit fault of the transformer. By combining technical means of multiple subjects and multiple majors, the purpose of accurately evaluating the state of the transformer after the transformer bears short-circuit impact is achieved, the analysis time of technicians is greatly shortened, the result analysis accuracy is improved, the safe and stable running of the transformer and a power grid is ensured, and the running reliability and effectiveness of the power grid are ensured.

The invention relates to a quality evaluation method for a dendrobe medicinal material. The method includes the steps that a schaftoside chromatographic peak is adopted as a reference substance, and a standard fingerprint spectrum is formulated through HPLC (high performance liquid chromatography); through comparison of fingerprint spectrums of samples for test and the standard fingerprint spectrum, the quality of the samples for test is determined. According to the method, the pretreatment method for all the samples for test is simple, characteristic components are reserved, and the solution stability of the samples for test is good. The high performance liquid chromatography is high in precision, good in reproducibility and short in analysis time, and has certain specificity, the separation effect of all fingerprint peaks in the obtained fingerprint spectrum is good, the quality of the dendrobe medicinal material can be accurately and comprehensively reflected, the quality standard of the dendrobe medicinal material is further perfected, and therefore the quality evaluation method can be applied to quality grading and authenticity of dendrobe tissue culture seedlings, fresh dendrobe and air-dried dendrobe of medicinal dendrobe.

The invention relates to a frictional wear CAE (Computer Aided Engineering) analysis-based mold optimization method, and belongs to the technical field of stamping dies of automotive structural parts. The method comprises the following steps: (1) establishing a mesh model and determining a material model of a plate and a mold and an Archard wear model; (2) defining conditions such as a material parameter, a motion parameter, a constraint condition, simulation steps and working environment parameters by combining actual working conditions; (3) performing simulation calculation under the conditions defined in the step (2) to obtain a plate forming result and a model wear result; (4) changing a mold hardness parameter, a mold material parameter and the motion parameter respectively under the condition that other simulation conditions are not changed to perform calculation so as to obtain wearing capacity of the mold and analyze the influence of different parameters on mold wear; (5) supposing the mold wear every thousand times as a constant value, predicting service life before mold repair; (6) obtaining actual wear after the mold wear in production through a test method and verifying the feasibility of the CAE analysis method.

The invention provides a method for analyzing a microbial population function by using metagenome data. The method comprises the following steps: collecting all known microbial species, gene and function information, and integrating the information as a reference database; sequencing the metagenome of the to-be-tested microbial specifies, controlling the sequencing data quality, computing speciesabundance and gene abundance, analyzing the composition difference of microbes among different samples and gene level difference; annotating the gene function, clustering the genes with the same function to obtain a function module, performing adduction computation on related abundance of all non-redundancy genes in various function modules to obtain abundance values of all function modules, performing difference comparison analysis or overall evaluation on the functions of the to-be-tested sample microbes. Through the method provided by the invention, the step of respectively comparing the splicing data, the assembling data, the predicting data and the sequencing data with the single function database is saved, the time is saved, the utilization efficiency of the sequencing data is improved, and the method can be used for analyzing high-throughput microbe whole-genome sequencing data and screening the function microbe.

The invention relates to the field of detection of harmful components in foods and particularly relates to a method for determining the content of biogenic amines in foods by adopting an ultra-efficient bonded phase chromatography. The method comprises the following steps: carrying out biogenic amines extraction treatment on foods to be detected with the mass of m by using an HClO4 solution to obtain liquid supernatant with the volume of V; adding an n-hexane solution with the equal volume into the liquid supernatant, extracting, and repeating for 1-2 times; removing an upper-layer organic phase part to obtain a purified extracting solution; carrying out pre-column derivatization on the purified extracting solution to obtain a derivatized extracting solution; and detecting the content of the biogenic amines in the derivatized extracting solution by using an ultra-efficient bonded phase chromatographic instrument to obtain the mass concentration of the biogenic amines in the extracting solution. According to the method, the content of the biogenic amines in the foods can be determined by adopting the ultra-efficient bonded phase chromatography and a mixed system of supercritical carbon dioxide and n-hexane / isopropanol / an ammonia water solution is used as a flowing phase; the viscosity is low, the mass transfer performance is good, the analyzing time is rapid, the solvent loading amount is samll and the environmental pollution is small.

The invention relates to a system for automatically acquiring and analyzing a trace gas, which comprises a central control computer, an industrial control module group, a sampling part and a gas acquisition and analysis part, wherein the central control computer is used for writing programs by means of industrial control configuration software, generating and transmitting tank cover, gas passage and gas analysis commands, and receiving and storing analog signals which contain environmental meteorologic parameters and trace gas concentration data and are transmitted by the industrial control module group; the industrial control module group is respectively connected with the central control computer, the sampling part and the gas acquisition and analysis part; the gas acquisition and analysis part is connected with a pipeline of the sampling part; the industrial control module group is used for receiving industrial control commands, outputting multiple switching signals, and controlling the sampling part and the gas acquisition and analysis part to automatically carry out tank cover switching, gas acquisition and trace gas concentration analysis according to the programs; and the industrial control module group is used for processing current signals output by the sampling part and voltage signals output by the gas acquisition and analysis part and outputting the analog signals containing the environmental meteorologic parameters and the gas concentration data.

The invention discloses a simple, fast and sensitive method for detecting a plasticizer (phthalate ester) in plastic, which is based on ion mobility spectrometry and detects phthalate ester in an extracting solution using a fast ion mobility spectrometry detector in combination with a simple organic solvent extraction pretreatment method. The measurement method is simple, fast and reliable. The analysis time of the method is only 0.5min, the requirement on a sample pretreatment is low, the detection time is short, the sensitivity is high, and the detection limit can be up to 1ppb.

An ellipsometer measures any point of a sample by a first spectrometer and a second spectrometer. The ellipsometer performs analysis based on the measurement results obtained by the first spectrometer, performs analysis based on the measurement results obtained by the second spectrometer, and calculates an approximation formula for approximating the analysis results obtained by the second spectrometer to the analysis results obtained by the first spectrometer. The remaining points of the sample are measured with the second spectrometer, and the results of analysis using the measurement results are corrected based on the approximation formula.

The invention encompasses molecular beacon (MB) probes for monitoring the presence of human breast cancer biomarkers and for the analysis of breast cancer metastasis. The molecular beacon is an oligonucleotide probe which sensitively and specifically identifies biomarker mRNA in samples, in the presence of serum, in minimal time using fluorescence detection. The molecular beacons may be comprised in kits for the detection / quantitation of cancer biomarkers in clinical samples. The invention provides improvements in simplicity, accuracy, and speed over current methods, which could allow for improved patient treatment and prognoses.

The invention provides a solid phase and liquid phase compound dynamic gas preparation device and a gas preparation method. The gas preparation system comprises a diluted gas supplying portion, a diluted gas preheating portion, a sample standard liquid micro-scale control sample injector, a gas mixing portion and a gas analysis device. According to the gas preparation method, diluted gas passes through the diluted preheating device and enters a liquid evaporating device in a certain flow, meanwhile sample standard liquid enters the liquid evaporating device to mix with the diluted gas, finally the mixed gas passes through the gas mixing portion for being mixed evenly, a part of gas enters the gas analysis device for analysis and other gas is exhausted as tail gas. The flow ratio of sample gas and the diluted gas can be effectively controlled due to change of flow of the micro-scale sample injector and flow of the diluted gas so that the mixed gas with different concentrations can be obtained without preparation of the sample standard liquid in all concentration points and preparation efficiency and accuracy of solid phase and liquid phase compound standard gas are improved.

The invention provides a microwave plasma ordinary-pressure desorption ionization source for generating plasma by applying microwave energy. The microwave plasma ordinary-pressure desorption ionization source comprises a novel microwave device capable of generating plasma, a microwave power source and a coaxial cable. The microwave device comprises an outer pipe, an inner pipe, a middle pipe and an adjusting piston, wherein the outer pipe, the middle pipe and the inner pipe are in an three-pipe coaxial structure and are respectively provided with two working gas inlets, one end of the coaxial cable is connected with the microwave power source, and the other end of the coaxial cable is coupled to the middle pipe. The ionization source can be combined with a plurality of mass spectrometers with atmosphere ports for use, can carry out desorption ionization on solid, liquid and gas samples under the ordinary-pressure environment without sample pretreatment, poisonous chemical reagents, high voltage and high-speed airflow, can shorten the detection time to the samples, can realize the real-time, on-line and non-destructive detection on the sample, can save the detection cost, cannot pollute samples and is environment-friendly; ions of obtained products are mostly molecular ions; and the obtained spectrogram is concise and distinct.

The invention discloses a multi-wave wave field separation and synthesis method and a system. The method comprises the following steps: carrying out vector superposition on all wave vectors within a quadrant in which longitudinal wave / transverse waves are positioned in a certain time window; obtaining longitudinal wave / transverse wave unit wave vectors based on the superposed vector; at a moment in the time window, separating longitudinal wave / transverse wave vector along the R axis / Z axis respectively, forming an affine coordinate system in direction of the longitudinal wave / transverse wave vector, and converting the separated longitudinal wave / transverse wave vector to the affine coordinate system based on an included angle between the longitudinal wave / transverse wave vector and the R axis and an included angle between the longitudinal wave / transverse wave vector and the Z axis; and based on the unit wave vector, separating the longitudinal wave / transverse wave vector at a moment in the time window to obtain the parallel parts of the longitudinal wave / transverse wave vector and relevant unit wave vector under the affine coordinate system for output. The invention can separate the wave fields of the longitudinal wave vector and the transverse wave vector, recover the vector amplitude of the longitudinal wave / transverse wave, suppress noise, and improve accuracy of earthquake data analysis, thereby meeting need of earthquake data analysis and explanation.

The invention relates to a method for detecting illegally-added 42 chemicals in Chinese patent medicines and health care products simultaneously and belongs to the field of food safety. The detecting method disclosed by the invention is a high performance liquid chromatography-quadrupole / electrostatic field orbitrap high-resolution mass spectrum combined detecting method. According to the established method, the pretreatment process is simple and convenient, the analysis time is short, and the method is accurate, reliable, high in sensitivity and suitable for qualitative confirmation and quantitative detection of the illegally-added 42 chemicals in the Chinese patent medicines and the health care products and can be used for screening illegally-added drugs in the Chinese patent medicines and the health care products. Effective evidence is provided for control of quality of the health care products and fighting of illegal addition behaviors in the industry of health care products.

The invention provides a detection method for veterinary drug residues. The detection method comprises the following steps of (1) treating a sample: pretreating the sample to be detected, adding an extracting agent, and performing treatment by multiple steps to obtain supernate, wherein filtrate obtained by filtration on the supernate is used for UPLC-MS / MS (ultra performance liquid chromatography-mass spectrum / mass spectrum) measurement; (2) measuring the veterinary drug residues: performing UPLC-MS / MS measurement on the obtained sample under a certain measurement condition. The detection disclosed by the invention has the characteristics of high sensitivity and high specificity and can conform to the detection on the veterinary drug residues.

The invention discloses a method for quickly screening and detecting azo dyes forbidden in textile materials, leathers and dyes using gas chromatography mass spectrometry, which comprises the following steps: 1) preparing a sample, 2) subjecting the sample to reduction reaction, 3) extracting the sample, 4) performing the gas chromatography mass spectrometry on the sample, 5) screening decision: compared with the GC-MS analysis result of a standard solution, if the retention time and the mass spectrum of the aromatic amine in the sample keep with standards, the sample is judged as a suspicious positive sample, otherwise it is a negative sample and directly judged as a qualified sample. The sample pre-treatment time is shortened, the cost is reduced, the detection time is shortened, the operation step is simple, the operation is convenient, the operation time is short, the operation cost is low, the detection efficiency is improved; the aromatic amine in the sample is detected by a gas chromatography mass spectrometer, color interference problem is not existed, the sample matrix is effectively prevented from being infected, and the judgment accuracy is high, thus the method is not only suitable for textile materials, but also suitable for other products such as leathers and dyes.

The invention relates to a comprehensive scheduling and load balancing method for an intelligent massive video analysis system. A leading end is a client side, and a background comprises a comprehensive scheduling server, multiple video analysis servers and a file transfer server, wherein the comprehensive scheduling server is responsible for receiving analysis tasks of the client side, decomposing big data files according to file size, quantity and length of time and building a task mapping model; the video analysis servers report the operation states to the comprehensive scheduling server regularly; the comprehensive scheduling server allocates the tasks to the proper video analysis servers according to the priority of the analysis tasks and the states of the video analysis servers, and meanwhile, dynamically adjusts allocation results of the tasks according to the states of the video analysis servers; the file transfer server receives analysis results of the video analysis servers and synthesizes and transfers the analysis results to the client side.

The invention relates to the field of biomedicine, in particular to a method and a device for detecting tumor mutation load based on a single sample. According to the device, a probe combination comprises probes that capture the exon region of a gene shown in Table 1, an intron promoter fusion breakpoint region of a gene shown in Table 2, and a coding region region of a gene shown in Table 3. A human tumor polygene detection kit comprises the probe combination. The TMB detection method based on the single sample comprises the following steps: access to target area of the tumor samples under test sequencing data, with the reference genome comparison, based on comparing the results obtained, detecting mutation loci, filtering original variable results with normal sample normal baseline database coincidence loci, filtering the high frequency reproductive mutation locus of a first cell mutation data set, screening the clonal somatic cell mutation locus of a second somatic cell mutation data set and calculating TMB. This method can accurately detect TMB of tumor samples without pairing samples.

The present invention discloses a construction method for a compound thrombus clearing preparation bioactivity chromatography finger print. The construction method comprises the following steps: (1) preparing a compound thrombus clearing preparation test solution, precisely sucking 10 mul of the compound thrombus clearing preparation test solution, and injecting into a double-pump dual-gradient high performance liquid chromatograph to obtain a compound thrombus clearing preparation HPLC finger print; (2) preparing a mixed reference substance solution, precisely sucking 10 mul of the reference substance solution, and injecting into the double-pump dual-gradient high performance liquid chromatograph to obtain a reference substance HPLC finger print; (3) adopting a reference substance control method, and adopting an RRLC / MS / MS technical method to prove 21 chemical components in the finger print; and (4) analyzing correlation between the 21 chemical components and bioactivity difference, clarifying bioactivity component groups, and establishing a compound thrombus clearing preparation bioactivity chromatography finger print. According to the present invention, the compound thrombus clearing preparation HPLC finger print and the bioactivity component groups obtained from chromatograph efficiency analysis are combined to construct the compound thrombus clearing preparation bioactivity chromatography finger print so as to completely monitor safeties, effectiveness and quality uniformities of raw material herbs, semi-finished products and finished products.

The present invention discloses filter paper sheet gas chromatography-mass spectrum analysis process of organic acid / amino acid metabolic product in urine. The process includes elution of urine collected with filter paper, oximation reaction, extraction, methyl silane derivation, and united detection with chromatographic instrument and mass spectrograph. The process of the present invention is superior in that the filter paper collection of urine favors remote analysis of genetic metabolic diseases; the oximation reaction processing favors alpha-ketonic acid detection for diagnosis of branched chain ketonic acid metabolic disorder; the extraction has high recovery rate of organic acid / amino acid metabolic product, high repeatability, less chromatographic impurity peaks and no pollution to chromatographic capillary column and mass spectrographic ion source; and the united detection is suitable for screening analysis of great amount of samples.

The invention relates to a method for separating an anthocyanins monomer from lycium ruthenicum fruits. The method comprises the following steps: (1) pretreating raw materials: removing impurities from lycium ruthenicum fruits, washing the lycium ruthenicum fruits with water to obtain the treated lycium ruthenicum fruits; (2) preparing a formic acid-methanol solution at a volume concentration of 2%; (3) adding the formic acid-methanol solution into the lycium ruthenicum fruits for extraction at room temperature to obtain an extract; (4) filtering, and concentrating the extract by evaporating to obtain an lycium ruthenicum anthocyanins extract; (5) treating macroporous adsorption resin; (6) redissolving the lycium ruthenicum anthocyanins extract, applying the redissolved lycium ruthenicum anthocyanins extract to the treated macroporous adsorption resin, and collecting effluent; (7) concentrating the effluent under reduced pressure to obtain crude anthocyanins; (8) injecting the crude anthocyanins into a high-performance semipreparative chromatographic column, and separating to obtain the anthocyanins monomer with the purity of higher than or equal to 90%; concentrating the anthocyanins monomer under reduced pressure, and performing freeze drying in vacuum to constant weight to obtain finished anthocyanins monomer. The method is good in separating effect, short in time consumption and low in cost.

A field portable mass spectrometer system comprising a sample collector and a sample transporter. The sample transporter interfaces with the sample collector to receive sample deposits thereon. The system further comprises a time of flight (TOF) mass spectrometer. The time of flight mass spectrometer has a sealable opening that receives the sample transported via the sample transporter in an extraction region of the mass spectrometer. The system further comprises a control unit that processes a time series output by the mass spectrometer for a received sample and identifies one or more agents contained in the sample.

The invention relates to the field of analytical chemistry and discloses a method for rapidly detecting a main component in active lime and passivated lime. The method comprises the following steps: 1, weighing a proper amount of active lime and passivated lime samples and carrying out a blank test along with the samples; placing the samples into a heating container; adding a proper amount of hydrochloric acid solution; heating till the samples are completely decomposed; cooling a few undissolved substances of the passivated lime sample to the room temperature; after the solution is filled into a volumetric flask to fix volume, respectively extracting two parts of proper amount of test solution; and 2, respectively titrating calcium oxide and magnesium oxide or calcium and magnesium. Theinvention has the characteristics that the sample decomposition is simplified, and a measurement error is reduced and the analysis time is shortened by controlling the titer of EDTA and EGTA titrand within the burette volume range; two separately added indicating agents are prepared into a mixed indicating agent to be added once when the calcium and magnesium or magnesium oxide is directly titrated so as to reduce the operation time; and the final result is obtained by adding the experience correction coefficient in the passivated lime sample.