The invention discloses a micro-fluidic
chip immunoassay kit and a detection method thereof. The kit comprises a micro-fluidic
chip, a
wafer cover plate and a
wafer bottom plate, wherein the
wafer cover plate and the wafer bottom plate are bonded up and down. One or more detection units are arranged in the wafer bottom plate, and each detection unit comprises a
liquid storage tank, a mixed reaction tank, a detection tank, a waste
liquid tank and a first vent hole which are connected in sequence; the mixed reaction tank is fixedly provided with a fluorescent probe corresponding to a detection
drug; goat anti-mouse immune
globulin G and corresponding
drug antigens are fixed in the detection tank. The invention also discloses a detection method using the kit. The method has the characteristics of
high flux, low sample consumption, high detection speed, simplicity and convenience in operation and the like; the method has the advantages of no need of an
electrode, a
voltage or an external
magnetic field, no need of electroosmosis driving, hot
air pump driving or optical capture
micropump driving, simultaneous detection of various
veterinary drug residues in various matrixes, suitableness for on-site rapid
screening analysis, and great alleviation of the detection pressure of related detection personnel.