98 results about "Repeat sequence" patented technology

Translation modulating agents that modulate expression of one or more translation start sites for expanded repeat (e.g., DPR) protein synthesis are provided. Compositions and methods for treating translation start sites for expanded repeat (e.g., DPR) protein synthesis-associated disorders are also provided.

The disclosure provides methods for the diagnosis of Huntington's disease. In some embodiments, the method comprises detecting one or more repeat associated non-ATG (RAN) proteins in a biological sample.

The invention discloses a bimodal polymyxin drug-resistant gene MCR-1 detection platform, relates to the field of food safety and analytical chemistry, and in particular relates to an on-site rapid detection method of the polymyxin drug-resistant gene MCR-1 in a food sample, primer groups aiming at the polymyxin drug-resistant gene MCR-1 are respectively as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5, and a sixth primer with the 5'end labeled by biotin; the CRISPR / dCas9 system comprises dCas9 protein and sgRNA, and the sequence of the sgRNA is marked as SEQ ID NO.7; the sequence of the gold-platinum nanoprobe is marked as SEQ ID NO.8; the LAMP technology is adopted to amplify the polymyxin drug-resistant gene MCR-1 in the sample to be detected, an LAMP product contains multiple sections of reverse circulation repetitive sequences, and each double strand can be combined with more gold-platinum nanoparticles, so that sensitive detection of the food-borne pathogenic bacteria containing the polymyxin drug-resistant gene MCR-1 in the food sample is realized, the sensitivity is relatively high, the detection time is short, the detection time is short, and the cost is low. The operation is simple, the stability is high, and the whole detection process can be completed in a short time.

The present invention provides a method for constructing a plasmid containing a tandem repeat sequence, comprising: providing a first upstream primer and a first downstream primer; the first upstream primer and the first downstream primer respectively have a tandem repeat sequence, and the tandem repeat sequence of the first upstream primer is complementary to at least part of the tandem repeat sequence in the tandem repeat sequence of the first downstream primer; annealing the first upstream primer and the first downstream primer under a gradient temperature condition to obtain a first double-stranded nucleic acid fragment having a tandem repeat sequence, wherein the tandem repeat sequence complementary to each other in the double strand is the first double-stranded tandem repeat sequence; connecting the first double-stranded nucleic acid fragment to a first vector backbone with a sticky end to construct a plasmid containing the first double-stranded tandem repeat sequence. The present invention can significantly improve the success efficiency of tandem repeat sequence connection, obtain more positive clones, ensure the success rate of the experiment, and reduce the construction time.

A prompt for a generative artificial intelligence (GenAI) model which contains unicode is received. The prompt is then tokenized to result in a plurality of tokens. Token forming part of a repeating sequence are identified and then removed to result in a modified set of tokens. The modified set of tokens are subsequently detokenized to result in a modified prompt. It is then determined, whether ingestion of the modified prompt by the GenAI model will result in the GenAI model behaving in an undesired manner. The modified prompt is passed to the GenAI model when it is determined that ingestion of the modified prompt will not result in the GenAI model behaving in an undesired manner. Otherwise, at least one remediation action is initiated when it is determined that ingestion of the modified prompt by the GenAI model will result in the GenAI model behaving in an undesired manner.

Aspects of the disclosure relate to compositions and methods for the diagnosis and / or treatment of certain neurodegenerative diseases, for example those diseases associated with repeat-associated non-ATG (RAN) translation proteins, such as amyotrophic lateral sclerosis (ALS). In some embodiments, the disclosure relates to recombinant adeno-associated viruses (rAAVs) expressing antibodies and antigen-binding fragments thereof that bind to poly(GA) RAN proteins. In some embodiments, the disclosure relates to methods of treating a RAN protein-associated disease by administering to a subject in need thereof the rAAVs.

To provide a composition and method for modifying primary T cells.SOLUTION: The present invention is a method of gene editing comprising administering stem-loop CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) RNA (st-crRNA) and Cpf1 enzyme to a cell.SELECTED DRAWING: None

The invention discloses a sweet potato and its closely related species centromere-specific repeat sequence and its application. The sweet potato is sequenced by second-generation sequencing and third-generation sequencing technology, and the sweet potato centromere-specific repeat sequence is obtained by analysis. After comparative analysis, identification and optimization, the tandem repeat sequence unique to the sweet potato centromere is obtained. After the sequence is designed, the fluorescent signal is marked and converted into a centromere probe, which proves that it can generate clear and bright centromere region hybridization signals in the FISH experiment of sweet potato and closely related species, and accurately display the position of the centromere on the chromosome. By counting the number of centromere signals, the accurate counting of the chromosomes of sweet potato and closely related species can be achieved.

The present invention is a precursor crRNA for a type I CRISPR-Cas system having a modified nucleotide, in which at least one nucleotide is a modified nucleotide in a region comprising the 5'arm region of a first repeat sequence, the 5 '-side stem formation region of the first repeat sequence, and the ring formation region of the first repeat sequence.

The invention provides an information bit transmission method and a multiplicative repetitive sequence determination method, which are applied to the technical field of communication, and the information bit transmission method comprises the following steps: obtaining a first symbol sequence which is obtained by coding a first information bit; multiplying the first symbol sequence by each multiplicative repetition factor in the target multiplicative repetition sequence to obtain a plurality of second symbol sequences; and modulating the plurality of second symbol sequences, and transmitting the obtained plurality of first modulation sequences, the plurality of first modulation sequences being used for enabling the opposite end to determine the first information bit. After the first symbol sequence corresponding to the first information bit is obtained, the first symbol sequence is multiplied by the target multiplicative repetitive sequence, so that the purpose of performing multiplicative repetitive transmission on the first symbol sequence for multiple times can be achieved. Compared with a direct repeated transmission mode in the prior art, the multiplicative repeated transmission mode can improve the transmission performance.

Split-pegRNA recorder reagents and methods are provided that provide specific molecular interactions within cells induce precise genome editing that inserts an event-specific barcode sequence into the predetermined genomic locus of DNA Tape. The split pegRNAs include (a) a crRNA component, including in 5′ to 3′ order (i) a spacer sequence for a genomic locus of interest; (ii) a crRNA repeat sequence necessary for binding to a CRISPR-Cas protein; and (iii) a first RNA extension; and (b) a petracrRNA component, including in 5′ to 3′ order (i) a second RNA extension; (ii) a petracrRNA antirepeat sequence necessary for binding to a CRISPR-Cas protein; (iii) a gRNA scaffold; wherein the crRNA component and the petracrRNA component are not covalently bound to each other.

A method for improving the replication of covalent closed circular plasmids is provided. [Solution] The method comprises the step of preparing a covalent closed circular plasmid having a Pol I-dependent origin of replication and an insert containing a structured DNA sequence selected from the group consisting of a reverse repeat sequence, a directional repeat sequence, a homopolymer repeat sequence, a eukaryotic origin of replication, or a eukaryotic promoter enhancer sequence, wherein the structured DNA sequence is located at a distance of less than 1000 bp from the Pol I-dependent origin of replication in the direction of replication. The method also comprises the step of modifying the covalent closed circular recombinant molecule so that the Pol I-dependent origin of replication is replaced with a Pol III-dependent origin of replication, thereby improving the replication of the covalent closed circular plasmid with the resulting Pol III-dependent origin of replication. A covalent closed circular recombinant DNA molecule without antibiotic markers is also provided.

It is an object of the present invention to provide a method for modifying multiple DNAs encoding identical or similar proteins using TALE, when the multiple DNAs are present. More specifically, the present invention relates to a method for modifying multiple DNAs encoding identical or similar proteins, wherein the method comprises allowing the TALE portion of one type of TALE-modifier complex comprising at least one repeat sequence containing RVD (repeat variable di-residue) composed of amino acids that recognize or tolerate N, V, H, D, B, R, Y, M, W, S or K, to bind to the binding regions of the multiple DNAs.

The present invention relates to a conjugate of a specific cell penetrating peptide covalently linked to a therapeutic antisense oligonucleotide having a nucleotide repeat sequence, suitable for treatment of repeat expansion based disorders, in particular myotonic dystrophy (DM1).

Provided herein are methods for determining the microsatellite instability status of samples. In one aspect, the methods include quantifying a number of different repeat lengths present at each of a plurality of microsatellite loci from sequence information to generate a site score for each of the plurality of the microsatellite loci. The methods also include comparing the site score of a given microsatellite locus to a site specific trained threshold for the given microsatellite locus for each of the plurality of the microsatellite loci and calling the given microsatellite locus as being unstable when the site score of the given microsatellite locus exceeds the site specific trained threshold for the given microsatellite locus to generate a microsatellite instability score, which includes a number of unstable microsatellite loci from the plurality of the microsatellite loci.

The present invention relates to the field of biotechnology, and discloses a novel Cas13a / cr:mi:crRNA gene knockdown tool, a construction method and an application thereof. Two regularly clustered short palindromic repeat sequences RNAa (CRISPR-Derived RNA, crRNAa) and regularly clustered short palindromic repeat sequences RNAa (CRISPR-Derived RNA, crRNAb) sequences that guide Cas13a nuclease (Cas13a) to a target gene are respectively connected to the left and right wings of a primary miRNA (pri-miRNA) to form a cr:mi:crRNA guide RNA that triple-recognizes a target gene, and constructs Cas13a / cr:mi:crRNA. After bovine and mouse muscle cells are transfected with Cas13a / cr:mi:crRNA, cr:mi:crRNA is cut and separated with the help of Drosha nuclease to produce crRNAa, crRNAb and precursor miRNA (Previous miRNA, pre-miRNA) separated from each other, thereby performing triple recognition and cutting of the targeted messenger RNA (Messenger RNA, mRNA), significantly improving the efficiency of RNAi and providing an efficient gene interference system for biological breeding.

The present application relates to the technical field of biological research data analysis, and discloses a kind of computing biology research data analysis display method, including extracting total RNA from biological tissue or cell, RNA reverse transcription is cDNA, constructs library, and utilizes high-throughput sequencing platform to sequence library, to obtain a large number of short sequences.The present application can effectively identify abnormal repeat regions by comparing and threshold judging the number of short sequences mapped to the same genomic coordinate interval, so as to determine whether the gene expression difference result is reliable, when the number of abnormal sequences exceeds the preset repeat sequence threshold, by eliminating the influence of repeat sequence operation, i.e. for the case of high FPKM value, remove the completely same and overlapping short sequences, and re-align the updated results, the expression distortion problem caused by repeat counting can be corrected.

To provide more active hyperactive transposons and transposases.SOLUTION: The present invention relates to a polypeptide comprising a piggyBac transposase or a fragment or a derivative thereof having a transposase function comprising at least one amino acid substitution. Further, the present invention relates to a transposable element comprising a piggyBac or piggyBac-like left repeat sequence and left internal repeat sequence, where the left internal repeat sequence comprises at least one nucleotide modification. Furthermore, the present invention relates to a kit comprising the transposase and / or transposable element. In addition, the present invention relates to a targeting system comprising the transposase and / or transposable element.SELECTED DRAWING: None

RNA molecules comprising a guide sequence portion having 17-50 contiguous nucleotides containing nucleotides in the sequence set forth in any one of SEQ ID NOs: 1-2325 and compositions, methods, and uses thereof.

The present invention provides a method for preparing a blocking reagent for a repetitive sequence by using a multiple substitution amplification technique, the method comprising: amplifying genomic DNA by using a multiple substitution amplification technique; purifying the amplified product, breaking it into DNA fragments of 200-300 bp in length, and the resulting product is a blocking reagent for the repetitive sequence. The method of the present invention is used to prepare a blocking reagent for a repetitive sequence, which can avoid the disadvantages of the prior art method that placental DNA is not easy to obtain and the HAP column separation method is cumbersome, time-consuming and labor-intensive.

The invention relates to the technical field of genomics and microbial science, in particular to a myxobacteria T2T genome assembling method. The assembling method mainly comprises the four steps of global assembling, telomere sequence screening, local sequence clustering screening and second-generation data multi-round correction. According to the method provided by the invention, pollution removal strategies of telomere sequence screening and local sequence clustering screening are newly added, so that the problem that tandem repeat sequences in a myxobacteria genome are complex and short is effectively solved, the analysis of a complex region becomes possible, the integrity and continuity of assembly are improved, and the assembly efficiency is improved. The method does not need fingerprint spectrum, Hi-C technology or optical spectrum sequencing technology, reduces the experiment cost, ensures that all the sequences belonging to myxobacteria are extracted, effectively removes the interference of repetitive sequences and deep pronucleus pollution, enables highly similar repetitive sequence fragments to be reduced and positioned to an accurate genome position, and improves the accuracy of the detection result. The integrity and continuity of the genome are obviously improved.

The present application relates to the technical field of biological information, and particularly relates to a repeat sequence detection method, device and equipment and a storage medium, the repeat sequence detection method comprising the following steps: reading double-end current sequences from sequencing data obtained by double-end sequencing, and converting a first sequence segment in the double-end current sequences into a first integer type and a second sequence segment in the double-end current sequences into a second integer type; calculating a double-end sequence positioning characteristic value according to the first integer type, and detecting whether a value at a first position corresponding to the double-end sequence positioning characteristic value in a preset counting array is an occupied value; if the value at the first position is the occupied value, detecting whether the second integer type exists in a double-end sequence array; and if the second integer type exists in the double-end sequence array, determining that the double-end current sequences are repeat sequences. The present application improves the accuracy of repeat sequences.

The invention discloses a QPCR (Quantitative Polymerase Chain Reaction)-based DNA (Deoxyribose Nucleic Acid) methylation rate detection method, which comprises the following steps of: treating a DNA sample to be detected by bisulfite, dividing the DNA sample into a first sample and a second sample which are parallel to each other, and performing substrate amplification on a methylated DNA template and a non-methylated DNA template in the samples through PCR amplification by adopting the same amplification primer under the same condition; then respectively depleting dATP in a sample A reaction system and dGTP in a sample B reaction system through a template and a primer with thymine deoxyribonucleotide repetitive sequences or a template and a primer with cytosine deoxyribonucleotide repetitive sequences, and controlling independent detection of methylation and non-methylation templates through a d3TP system lacking dATP or dGTP; and finally, directly analyzing the fluorescence signal of the methylation template and the fluorescence signal of the non-methylation template, and calculating the methylation rate of the DNA sample to be detected through a methylation rate calculation function.

The invention relates to the field of molecular biology, in particular to a primer pair, a kit and a detection method for detecting a GAA repetitive sequence of an FXN gene. The sequence of the primer pair is as follows: FXN-F: 5 '-CCACCGTGTTATTTGGCC-3'; the primer pair is FXN-R: 5 '-CATGGCCACACCTGCCT-3', and the 5'end of the reverse primer FXN-R of the primer pair is provided with an FAM fluorophore. The method comprises the following steps: extracting DNA (Deoxyribonucleic Acid) and carrying out PCR (Polymerase Chain Reaction) amplification by utilizing the primer pair to obtain a PCR product containing a GAA trinucleotide repetitive sequence; the PCR product is detected through capillary electrophoresis, the GAA repetitive sequence condition of the FXN gene is analyzed according to the electrophoresis detection result, the FXN gene GAA repetitive sequence number is obtained, the whole detection process is short in time consumption, high in efficiency and high in specificity, and the trinucleotide repetitive sequence can be detected without sequencing.

This invention relates to the field of biomedical technology, specifically to a rapid and sensitive detection method for the CGG repeat sequence of the FMR1 gene and its application. It includes a primer combination for detecting the CGG repeat sequence of the FMR1 gene, comprising an upstream primer with the nucleotide sequence shown in SEQ ID NO: 1 and a downstream primer with the nucleotide sequence shown in SEQ ID NO: 2. This invention optimizes the PCR detection procedure, making it more sensitive for samples with high CGG repeat counts, while also enabling accurate quantification and faster detection, significantly shortening the detection time, effectively reducing the workload of operators, and lowering detection costs.

To provide compositions and methods for the diagnosis and / or treatment of certain neurodegenerative diseases, e.g., diseases associated with repeat-associated non-ATG (RAN) translation proteins, such as Alzheimer's disease (AD).SOLUTION: In some embodiments, the disclosure relates to identifying a subject having a RAN protein-associated disease by detecting expression or activity of repeat-associated non-ATG (RAN) translation proteins (e.g., RAN proteins). In some embodiments, the disclosure relates to methods of treating a RAN protein-associated disease by administering, to a subject in need thereof, an agent that reduces expression or activity of RAN proteins.SELECTED DRAWING: Figure 7

The invention provides a crop variety analysis method, device and equipment based on a simple repetitive sequence, and relates to the technical field of crop analysis, the method comprises the following steps: determining a target primer group according to a pre-screened simple repetitive sequence site of a target crop; performing polymerase chain reaction amplification on the target primer group to obtain an amplification product corresponding to the target primer group; performing electrophoretic separation on the amplification product to obtain a target fluorescence signal image corresponding to the amplification product; extracting target image features of the target fluorescence signal image; inputting the target image features into a pre-trained random forest classifier to obtain a variety analysis result of the target crop output by the random forest classifier; the random forest classifier is obtained by training based on the training image features and the corresponding variety identifiers. According to the method, feature extraction is performed on the target fluorescence signal image, and the target image features are input into the pre-trained random forest classifier, so that crop variety analysis can be accurately, efficiently and reliably realized.

The invention relates to a plasmid-free gene editing system based on T7RNA polymerase and application of the plasmid-free gene editing system, and belongs to the technical field of gene editing. The plasmid-free gene editing system constructed by the invention comprises a T7 RNA polymerase, a Cas12a nuclease, a selection marker gene and a short double-stranded DNA expression cassette, wherein the short double-stranded DNA expression cassette sequentially comprises a T7 promoter, a first forward repetitive sequence, a spacer sequence and a second forward repetitive sequence. According to the plasmid-free gene editing system, T7RNA polymerase is expressed in host bacteria, short double-stranded DNA is transcribed to generate guide RNA, Cas12a nuclease is driven to achieve rapid and efficient gene editing, the problems that in the prior art, linear fragment transformation is complex and tedious, and part of strains lack genetic plasmids are solved, iterative editing and multi-site editing are supported at the same time, and the plasmid-free gene editing system is suitable for large-scale popularization and application. And a brand-new convenient tool is provided for gene editing of non-model fungi.

The present application discloses a sequence analysis method, device and medium thereof, which relate to the field of biotechnology, and are used for sequence analysis. In view of the problems currently caused by the non-interoperability between software and hardware of different sequence analysis methods, a sequence analysis method is provided, which realizes sequence alignment independently and stores the obtained sequence alignment results so as to perform subsequent sequence analysis operations. Since the sequence alignment part is common to each sequence analysis method, the sequence alignment in different sequence analysis algorithms can be realized by the same software or hardware, without repeated design, and can also support the database of any sequence analysis algorithm, which is more conducive to practical implementation. In addition, sequence alignment is the part with the largest amount of calculation in each sequence analysis algorithm, and the efficiency of sequence analysis can be greatly improved without repeating sequence alignment, thereby improving the efficiency of biomolecule design.

The present invention provides for the production of improved viral and non-viral nanoplasmid vectors. Specifically, the invention provides an antibiotic-label-free covalent closed circular recombinant DNA molecule comprising: a. An antibiotic-label-free insert comprising a structured DNA sequence selected from the group consisting of a reverse repeat, a forward repeat, a homopolymerized repeat and an eukaryotic origin of replication; b. A Pol III-dependent origin of replication comprising an R6K [gamma] origin of replication having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 18; and c. An RNA-OUT RNA selection marker, which comprises an RNA-IN modulating RNA-OUT RNA having at least 95% sequence identity with SEQ ID NO: 6, and a method for detecting the RNA-OUT RNA.