The invention discloses a rapid automatic focusing module and method suitable for a
fluorescence microscope. The automatic focusing module comprises a
laser, a diaphragm, a collimating lens group, a reflector, a dichroic mirror, an objective lens, a sample to be measured, a
telescope lens group, a CCD2
detector, a
control system and a displacement platform which are sequentially arranged along a focusing light path, and the sample to be measured, the objective lens, the dichroic mirror, a tube lens and the CCD2
detector which are sequentially arranged along an imaging light path; the collimated
laser beam is emitted to the plane where the sample to be detected is located through the objective lens, and then the
laser beam is reflected back to the
telephoto lens group and converged to the CCD2
detector. According to the offset of the center of the reflected
light beam, the relative distance between the objective lens and the to-be-tested sample is moved through a
control system, and automatic focusing of a
single view field of the to-be-tested sample is achieved; the to-be-measured sample is moved to the focal planes of different
object view fields in the X direction and the Y direction and is quickly focused in real time, so that the large-size to-be-measured sample is always kept at the focal plane position, and the
image quality and the working efficiency are improved.