365 results about "Biological product" patented technology

The invention discloses a toxoplasma gondii attenuated vaccine strain RH delta rop67 as well as a construction method and application thereof, and belongs to the technical field of parasitic disease prevention and control and biological product preparation. The attenuated vaccine strain is constructed by performing targeted knockout on the ROP67 gene in a toxoplasma gondii strain RH delta ku80 through a CRISPR / Cas9 mediated gene editing technology. Compared with a wild type strain, the attenuated vaccine strain shows remarkable attenuation characteristic and good immunogenicity. A test result shows that the attenuated vaccine strain can induce a host to generate specific immune response mainly based on cellular immunity, maintains a protection effect on toxoplasma gondii infection in a relatively long immune period, and has a protection effect on tachyzoite infection and a chronic infection stage of toxoplasma gondii strains with different virulence; the survival ability of a host to tachyzoite infection can be improved, and the formation level of cysts in tissues is reduced. The invention provides a technical scheme with long-term immune potential for research and development of toxoplasma gondii attenuated vaccines.

The invention belongs to the technical field of biological detection, and discloses a kit and a method for quantitatively detecting residual quantity of host cell protein of pichia pastoris in order to solve the problems of limit of quantitation and low detection sensitivity of an existing detection method. According to the present invention, the empty plasmid without the recombinant human serum albumin gene is introduced into the pichia pastoris, and purification is performed by combining the Protein A chromatographic column and the host cell protein coupling affinity column, such that the prepared polyclonal antibody can be effectively used for the quantitative detection of the residual amount of the pichia pastoris host cell protein in the biological product; the kit formed by the polyclonal antibody has good accuracy and repeatability on the quantitative detection of the host cell protein residues in the pichia pastoris source biological products, the quantification limit is 0.2 ng / mL, and the kit provided by the invention can meet the quantitative detection of the host cell protein residues in all the pichia pastoris source biological products.

The invention relates to the technical field of biology, in particular to application of a biological product expressed by an EsPTI15 gene to regulation and control of tiller number. The nucleotide sequence of the EsPTI15 gene is shown as SEQ ID NO: 1, it is found for the first time that the tiller number of EsPTI15 gene overexpressed siberian wildrye is remarkably increased compared with wild siberian wildrye, and the result proves that overexpression of the EsPTI15 gene can positively regulate and control the plant tiller development process. The discovery not only enriches the molecular mechanism theory of plant tillering regulation, but also verifies that the EsPTI15 gene has the application potential of increasing the tillering number of the plant, and provides key theoretical support and gene resources for subsequent technical research and development of introducing the EsPTI15 gene through gene operation, increasing the tillering number of forage grass and further realizing biomass improvement.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a biological product for overexpressing an OsARF7 gene to improvement of rice disease resistance, the nucleotide sequence of the OsARF7 gene is shown as SEQ ID NO.1, and the amino acid sequence of the OsARF7 gene is shown as SEQ ID NO.2. According to the invention, an OsARF7 overexpression rice strain is constructed by means of transgenosis. Tests show that the overexpression of the OsARF7 can significantly enhance the immunocompetence of the rice, for example, the expression level of a disease-resistant related gene is improved, active oxygen outbreak is significantly induced, and the resistance of the rice to false smut and bacterial leaf blight is significantly improved. The invention proves that the OsARF7 has a positive regulation effect on rice immunity, can be used for improving the disease resistance of rice, and has important significance for creating disease-resistant rice germplasm resources.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody combination for detecting porcine epidemic diarrhea virus (PEDV) S protein and its application. The combination comprises monoclonal antibodies 4E11 and 1F3. The heavy chain CDR1-3 (SEQ ID NO. 1-3) and light chain CDR1-3 (SEQ ID NO. 4-6) of 4E11, and the heavy chain CDR1-3 (SEQ ID NO. 7-9) and light chain CDR1-3 (SEQ ID NO. 10-12) of 1F3, together impart high specificity and affinity. Detection methods such as double-antibody sandwich ELISA constructed based on this combination, using 4E11 as the coating antibody and 1F3 as the labeling antibody, have advantages such as high sensitivity, strong specificity, and broad spectrum, and can be used for in vitro detection of PEDV S protein, biological sample analysis, and quality control of biological products.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting porcine epidemic diarrhea virus S protein and application of the monoclonal antibody combination. The combination comprises monoclonal antibodies 4E11 and 1F3, a heavy chain CDR1-3 (SEQ ID NO.1-3) and a light chain CDR1-3 (SEQ ID NO.4-6) of the 4E11, and a heavy chain CDR1-3 (SEQ ID NO.7-9) and a light chain CDR1-3 (SEQ ID NO.10-12) of the 1F3 jointly endow the combination with high specificity and affinity. The double-antibody sandwich ELISA and other detection methods constructed based on the combination take 4E11 as a coating antibody and 1F3 as a labeled antibody, have the advantages of high sensitivity, strong specificity, good broad spectrum and the like, and can be used for in-vitro detection of PEDV S protein, biological sample analysis and quality control of biological products.

The invention belongs to the field of biological products, and particularly relates to a bone glue product for preventing and treating osteoporosis and application, the bone glue comprises sheep bone glue (small molecules), pig bone glue (small molecules) and black pig bone glue (small molecules); five animals are beneficial, sheep and pigs are food beneficial to body health, bones are made into bone glue (small molecules) through modern production processes such as an enzyme preparation method and the like, the bone glue (small molecules) is easily dissolved in water and easily absorbed by a human body, can supplement colloid in the bones, is non-toxic and can be eaten for a long time; due to different yin and yang attributes, different types of osteoporosis patients can be prevented and treated; the traditional Chinese medicine also has good prevention and treatment effects on complications, fracture, hairline fracture, arthralgia and other bone diseases caused by osteoporosis; the compound can be applied to preparation of medicines, foods and health-care foods for preventing and treating osteoporosis.

The invention discloses a rhodotorula mucilaginosa strain as well as a fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms. The rhodotorula mucilaginosa BTN-HB-L28 strain is separated and purified from meconopsis in Lijiang of Yunnan, can be used in the field of cosmetics, has the effects of resisting skin oxidation, resisting aging, repairing skin and relieving skin, can be used as an active matter in skin care products, and has practical application value. The fermentation product filtrate of the strain has excellent HAS inhibition ability, and can significantly inhibit the expression of matrix metalloproteinase 3; the fibroblast proliferation can be obviously promoted under various concentrations; the proportion of SA-beta-Gal positive cells can be remarkably reduced under various concentrations, and the anti-aging effect is achieved; the antioxidant composition has remarkable DPPH and ABTS free radical scavenging capacity and has a good antioxidant effect; the secretion amount of cell inflammatory factors NO, IL-6 and TNF-alpha can be remarkably inhibited, and the good relieving effect is achieved.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The invention discloses preparation and application of a rabies virus G protein mRNA vaccine freeze-drying preparation, and relates to the field of veterinary biological products. According to the invention, rabies virus G protein mRNA is prepared on a large scale through an in-vitro transcription technology, and an mRNA-LNP (lipid nanoparticle) delivery system is successfully constructed by adopting a microfluidic encapsulation process. Through freeze-drying protective agent formula screening (including cane sugar, trehalose and mannitol) and freeze-drying procedure parameter optimization (including pre-freezing temperature, main drying rate and secondary drying residual moisture control), the freeze-dried mRNA vaccine preparation which can stably exist under the condition of 4 DEG C is finally obtained.

The invention belongs to the field of veterinary biological products, and relates to a porcine reproductive and respiratory syndrome virus strain and application thereof. The invention particularly discloses a porcine reproductive and respiratory syndrome virus strain with a microbial preservation number of CGMCC (China General Microbiological Culture Collection Center) No.47095. The invention also discloses a vaccine composition prepared from the strain. After pigs are inoculated with the vaccine composition, the vaccine composition can resist challenge of a porcine reproductive and respiratory integrated virus SD strain and a porcine reproductive and respiratory integrated virus HuN4 strain, and has application prospects.

The application discloses a kind of disposable bioreactor and method for preparing adenovirus harvest, belong to biological preparation technical field, the bioreactor includes reaction bag and magnetic stirring mechanism, the magnetic stirring mechanism includes stirring shaft, stirring vane, the number of the stirring vane is greater than 1 and is installed on stirring shaft in layers, the stirring vane includes second stirring vane and first stirring vane, the second stirring vane is fixed on stirring shaft, the first stirring vane is installed on stirring shaft by collar, further include linkage module and shear force transmission device, the linkage module is used to drive shear force transmission device action, realize shear force transmission device and collar connection and disengage.The method is realized based on the bioreactor.Adopting the present scheme, the stirring consistency of disposable bioreactor can be effectively guaranteed in the process of being used for expansion culture.

A system may select a first feature and a second feature of the biologic product. The system may determine a first biologic parent having the first feature and not having the second feature, wherein the first feature is based on an aspect of the first biologic parent. The system may determine a second biologic parent having the second feature and not having the first feature, wherein the second feature is based on an aspect of the first biologic parent, and the aspect of the second biologic parent can be combined with the aspect of the first biologic parent. The system may determine a biologic product having the first feature and the second feature, wherein the biologic product is determined based on an evaluation of a set of combinations of the aspect of the first biologic parent and the aspect of the second biologic parent.

1. Name of the designed product: controllable freeze-thaw machine (HDR-80W100T). 2. Use of the designed product: the designed product is used for freezing and thawing of biological products. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

This invention discloses a primer-probe combination, detection method, and kit for detecting human polyomaviruses, belonging to the field of molecular biology. The primer-probe combination includes primers with sequences as shown in SEQ ID NO. 1-28 and probes with sequences as shown in SEQ ID NO. 29-31. The detection method and kit constructed using this combination can simultaneously detect 14 human polyomaviruses (BKPyV, JCPyV, KIPyV, WUPyV, MCPyV, HPyV6, HPyV7, TSPyV, HPyV9, MWPyV, STLPyV, HPyV12, NJPyV, and LIPyV) in the same reaction tube. The human polyomavirus detection scheme provided by this invention has advantages such as wide coverage, high specificity, high sensitivity, and strong robustness, and is of great significance for the safety and quality control of biological products, especially cell products.

The invention relates to the technical field of biological product manufacturing, and particularly discloses an anti-shewanella-alginate egg yolk antibody as well as a preparation method and application thereof. The invention relates to an anti-shewanella algal egg yolk antibody, which is obtained by immunizing laying hens by using inactivated shewanella algal (RDSH-25070) thalli as antigens, collecting egg yolk liquid, separating and purifying, the shewanella algal (RDSH-25070) is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 14, 2025, is named as shewanella algal, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 14, 2025. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The anti-shewanella-alginate egg yolk antibody disclosed by the invention has a strong inhibiting effect on shewanella alginate, can be effectively applied to preventing and treating diseases caused by the shewanella alginate for aquatic animals, and can be used for effectively retaining freshness and preventing corrosion of aquatic products at the same time.

The invention provides a rabbit-source BCR sequence primer group based on high-throughput single cell V (D) J sequencing and application of the rabbit-source BCR sequence primer group. The primer group comprises a specific nested PCR (Polymerase Chain Reaction) primer pair (the sequence is as shown in SEQ ID NO.1-12) aiming at a rabbit BCR heavy chain (IgA / IgE / IgG / IgM) and a light chain (IgK / IgL). Through microfluid single-cell separation, nested PCR amplification and high-throughput sequencing, the naturally paired rabbit BCR full-length V (D) J sequence can be efficiently obtained. According to the rabbit-derived BCR sequence sequencing primer provided by the invention, B cell receptor library information of different tissue sources can be quickly and efficiently obtained. According to the method, paired antibody sequence information can be specifically obtained, B cell response diversity after rabbit immunization vaccine or pathogen infection can be better known, and important data support is provided for design, optimization, effect evaluation and the like of biological products.

The present application relates to the technical field of veterinary biological products, in particular to application of oridonin in preparation of poultry vaccine adjuvant. The poultry vaccine adjuvant is composed of oridonin, solvent and buffer. Experiments prove that after mixing the poultry vaccine adjuvant with poultry inactivated vaccine (such as Newcastle disease vaccine) for immunization, the humoral immune level of poultry can be significantly improved (HI titer of 21d after the second immunization is increased by more than 1.5 log2), and the secretion level of specific IgY (systemic immunity) and IgA (mucosal immunity) in serum can be extremely significantly up-regulated, and the high level of antibody is maintained for more than 4 weeks longer than that of the conventional vaccine group. Compared with commercial adjuvant ISA 201, the present application has better immune enhancement activity, high safety, no adverse reaction at injection site, and wide clinical application prospect.

The invention discloses application of ellagic acid in preparation of a medicine or a biological product for preventing or relieving ultraviolet radiation damage and aging, and belongs to the technical field of biological medicine and radiation protection. Experimental verification shows that ellagic acid with effective concentration can promote expression of antioxidant related genes, reduce expression of inflammation response genes and expression of apoptosis related genes, eliminate active oxygen and inhibit lipid peroxidation reaction, so that oxidative damage, inflammatory reaction and apoptosis caused by ultraviolet radiation are effectively relieved, and the anti-inflammatory effect of ellagic acid is improved. The stress tolerance of an organism is improved, and the aging health risk induced by ultraviolet radiation is relieved; the medicine or biological product can be applied before and / or after exposure of ultraviolet radiation, and is suitable for preventing or relieving oxidative damage and cell aging caused by ultraviolet radiation. The invention provides a new way for developing safe and effective ultraviolet radiation protection and anti-aging preparations.

The invention discloses a porcine epidemic diarrhea and porcine rotavirus (G9 type) bigeminy attenuated vaccine and a preparation method thereof, and relates to the technical field of veterinary biological products. According to the present invention, dominant genotype strains such as PEDV G2 type KQ01 strain (the preservation number is CCTCC NO: V202005) and PoRV G9 type CH09 strain (the preservation number is CCTCC NO: V202191) are adopted as parent strains, a cell continuous passage method is adopted to carry out passage attenuation, and the virus content, the purity, the specificity, the hereditary stability, the safety and the immunogenicity are systematically inspected during the passage process; and then, carrying out proliferation process research on passage attenuated strains PEDV HB17-R and PoRV JS01-R in a bioreactor, harvesting a cell culture, mixing the two antigens according to a proper ratio, adding a proper amount of a stabilizer, and carrying out a freeze vacuum drying process to obtain the high-purity PoRV-HB17-R / PoRV-HB17-R / PoRV-HB17-R / PoRV-HB17-R / PoRV-JS01-R composite antigen. The bigeminy attenuated vaccine is highly matched with current dominant prevalent strains (PEDV G2 type and PoRV G9 type), is based on a suspension culture technology and has both safety and high efficiency.

The invention relates to the technical field of aquatic animal immune regulation and control, in particular to rHoLL-like stepped lectin-like recombinant protein as well as a preparation method and application thereof. The recombinant protein has pathogen-related molecular pattern recognition and combination capabilities, can promote aggregation of pathogens in a body fluid environment, and activates an aquatic animal complement system, so that the non-specific immune defense capability is enhanced. The preparation method comprises the following steps: amplifying a HoLL-like mature peptide coding sequence, constructing a recombinant expression vector, and converting the recombinant expression vector into an escherichia coli expression host for induced expression to obtain rHoLL-like lectin-like recombinant protein existing in an inclusion body form; and carrying out affinity chromatography purification on the inclusion body protein, and carrying out renaturation treatment in a manner of gradient reduction of denaturant concentration to obtain the rHoLL-like stepped lectin-like recombinant protein. The rHoLL-like stepped lectin-like recombinant protein has good biological safety, and can be applied to aquaculture as an aquatic animal immunopotentiator or a related biological product.

The utility model relates to heat exchanger heat preservation technical field discloses a kind of biological product clean workshop detachable plate heat exchanger heat preservation device, including shell and plate heat exchanger, the shell includes box and the cover of being covered in the top mouth place of box, the plate heat exchanger is located in the sealed chamber formed by box and cover, pipeline is equipped on the plate heat exchanger, the pipeline is extended to the outside of shell after penetrating cover away from the one end of plate heat exchanger, heat preservation layer is equipped in the shell, and the heat preservation layer is located between plate heat exchanger and shell. The utility model aims at solving the problem that clean room plate heat exchanger produces condensate in biological product or vaccine production workshop, influences clean room environment, while also fully considers the cleaning and maintenance problem of plate heat exchanger, is easy to install maintenance dismounting while being convenient to clean, corrosion resistant.

Tangential flow filtration systems and methods for capturing and collecting biological products and related compositions are provided.

The invention relates to the field of research and development of veterinary biological products, and particularly provides a porcine rotavirus SCLS3 strain and application thereof. The invention discloses a porcine rotavirus SCLS3 strain which is preserved in China Center for Type Culture Collection on October 29, 2024, the preservation number is CCTCC NO: V202494, and the preservation address is Wuhan University, Wuhan, China. The vaccine composition prepared from the strain can prevent spreading of porcine rotavirus epidemic situation, and after the vaccine composition containing the strain is used for immunizing animals, the animal bodies can quickly generate antibodies, so that the vaccine composition has good prevention and control effects on current epidemic porcine rotavirus infection, and is good in biological safety.

This invention relates to the application of the lola gene as a target in the control of fall armyworm, belonging to the field of biotechnology for pest control. It involves the application of the lola gene as a target in pest control or the preparation of biological products for pest control. Using the fall armyworm as the research object, this invention uses the lola gene as a molecular target, combines CRISPR / Cas9 gene editing technology, and develops a highly efficient sgRNA target site for its knockout. By utilizing the effect of Lola in inhibiting the production of female eggs in fall armyworms, its reproductive capacity is reduced, thereby achieving the purpose of control. This method is convenient to operate, effective, and sensitive, enabling long-term effective control of pest populations. It also significantly reduces the negative environmental impact of chemical pesticide use and avoids the risk of pests developing resistance due to long-term exposure to chemical pesticides, providing an innovative solution for sustainable agriculture.

1. Name of the product in this design: Biological Product Transfer Box. 2. Purpose of this design: For the transport of biological products, to achieve recordability and monitorability during the transport process. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention provides a method for predicting the ultraviolet inactivation effect of viruses in a liquid biological product. The method comprises the following steps: (1) detecting the absorbance value of a to-be-detected liquid biological product at a preset wavelength; (2) constructing a relation curve of the ultraviolet dose of the BSA / virus mixed solution and the decline degree of the virus titer; (3) constructing a relation curve of the ultraviolet dose of the SMZ working solution and the SMZ degradation degree; (4) constructing a relation curve of the operation parameters and the SMZ degradability; and (5) substituting preset operation parameters into the relation curve of the operation parameters and the SMZ degradability, substituting the obtained result into the relation curve of the ultraviolet dose and the SMZ degradability, substituting the obtained result into the relation curve of the ultraviolet dose and the decline of the indication virus titer, and judging the ultraviolet inactivation effect of the virus according to a predicted value of the decline of the indication virus titer. The method is accurate in prediction result, does not need to consume liquid biological products, and can be used for various virus inactivation effects under the condition that the ultraviolet dose cannot be accurately calculated.

The utility model provides a kind of sampling equipment for biological product research and development, it is related to sampling equipment technical field, including: cabinet, gantry is fixedly installed in cabinet top, placing plate is fixedly installed in cabinet top, frame is equipped on placing plate, sampling needle cylinder is equipped in the inside of frame.This kind of sampling equipment can control sampling needle cylinder to sample by sampling mechanism, so it is unnecessary to sample manually, then be transferred into collection sample bottle, sample collection process is more simple and convenient, improve the stability of sampling, avoid reagent to leak and spill in transfer way, improve the progress of experimental work.

The invention provides a qPCR (quantitative polymerase chain reaction) primer probe group for detecting various nodaviruses, a detection method and application, and belongs to the technical field of molecular biological detection. Aiming at various nodaviruses capable of infecting insect cell lines, including animal shed virus, Tn5 cell line virus, black beetle virus, borawa virus, nodavirus and macrobrachium rosenbergii nodavirus, a set of primer probe combination is designed. In the combination, part of viruses share the upstream primer and the probe, so that synchronous detection and identification of multiple target viruses in one reaction system are realized. The invention also provides a kit containing the primer probe group and a detection method. The detection method provided by the invention has the advantages of wide coverage range, high detection sensitivity, strong specificity, good matrix interference resistance and the like, and is suitable for safety detection and quality control of nodavirus pollution in insect cell banks, cell cultures and related biological products thereof.

The invention discloses a preparation technology of astragalus polysaccharide camouflage monosaccharide, and relates to the technical field of biological product production, the preparation technology comprises the following steps: S1, extraction, S2, purification: carrying out purification treatment on astragalus polysaccharide extracted in the step 1, S3, degradation camouflage: dissolving the astragalus polysaccharide purified in the step 2 in a solvent, carrying out degradation treatment on the astragalus polysaccharide by adopting ultrasonic waves, and carrying out purification treatment on the astragalus polysaccharide to obtain the astragalus polysaccharide camouflage monosaccharide. A specific glucan structural unit in a polysaccharide chain is degraded and disguised into a monosaccharide or oligosaccharide structure; and S4, separating to obtain a product. Astragalus polysaccharide is directionally camouflaged as a monosaccharide / oligosaccharide structure, the recognition and uptake efficiency of astragalus polysaccharide by cancer cells is remarkably enhanced, so that the targeting of inducing apoptosis of cancer cells is improved, the structure can effectively activate and train immune cells such as dendritic cells and strengthen anti-tumor immune response, and meanwhile, the product can interfere with a metabolic pathway of cancer cells and reverse the drug resistance of cancer cells, so that the anti-tumor effect is improved. Nutrition supply is blocked to inhibit tumor growth, the production process is green and controllable, and good clinical transformation and industrialization prospects are achieved.