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567 results about "Unit operation" patented technology

In chemical engineering and related fields, a unit operation is a basic step in a process. Unit operations involve a physical change or chemical transformation such as separation, crystallization, evaporation, filtration, polymerization, isomerization, and other reactions. For example, in milk processing, homogenization, pasteurization, and packaging are each unit operations which are connected to create the overall process. A process may require many unit operations to obtain the desired product from the starting materials, or feedstocks.

Manufacturing process for the production of polypeptides expressed in insect cell-lines

The present invention provides a manufacturing method for polypeptides that are produced in insect cells using a baculoviral expression system. In one example, the insect cell culture is supplemented with a lipid mixture immediately prior to infection (e.g., one hour prior to infection). The polypeptides are isolated from the insect cell culture using a method that employs anion exchange or mixed-mode chromatography early in the purification process. This process step is useful to remove insect-cell derived endoglycanases and proteases and thus reduces the loss of desired polypeptide due to enzymatic degradation. In another example, mixed-mode chromatography is combined with dye-ligand affinity chromatography in a continuous-flow manner to allow for rapid processing of the insect-cell culture liquid and capture of the polypeptide. In yet another example, a polypeptide is isolated from an insect cell culture liquid using a process that combines hollow fiber filtration, mixed-mode chromatography and dye-ligand affinity in a single unit operation producing a polypeptide solution that is essentially free of endoglycanase and proteolytic activities. In a further example, the isolated polypeptides are glycopeptides having an insect specific glycosylation pattern, which are optionally conjugated to a modifying group, such as a polymer (e.g., PEG) using a glycosyltransferase and a modified nucleotide sugar.

Process for analyzing protein samples

InactiveUS7045296B2Reduce disulfide bondEasy labelingSamplingMicrobiological testing/measurementProtein expression profileUnit operation
Methods using gel electrophoresis and mass spectrometry for the rapid, quantitative analysis of proteins or protein function in mixtures of proteins derived from two or more samples in one unit operation are disclosed. In one embodiment the method includes (a) preparing an extract of proteins from each of at least two different samples; (b) providing a set of substantially chemically identical and differentially isotopically labeled protein reagents; (c) reacting the extract of proteins from different samples of step (a) with a different isotopically labeled reagent from the set of step (b) to provide two or more sets of isotopically differentially labeled proteins; (d) mixing each of the two or more sets of isotopically labeled proteins to form a single mixture of isotopically differentially labeled proteins; (e) electrophoresing the mixture of step (d) by an electrophoresing method capable of separating proteins within the mixture; (f) digesting at least a portion of one or more separated proteins of step (e) and (g) detecting the difference in the expression levels of the proteins in the two samples by mass spectrometry based on one or more peptides in the sample of labeled peptides. The analytical method can be used for qualitative and particularly for quantitative analysis of global protein expression profiles in cells and tissues, i.e. the quantitative analysis of proteomes.

Boiler combustion optimizing control system and optimizing control method based on accurate measurement system

The invention discloses a boiler combustion optimizing control system and a boiler combustion optimizing control method. The method comprises the following steps of: on the basis of the equilibrium distribution and transformation for coal powder in a fire coal unit, accurately measuring parameters such as wind, powder, ash and the like by using a measuring device; analyzing a history behaviour of operation by the deep analysis and digging of data by using the acquired real-time history data of a boiler and taking the work condition optimization as a basic optimizing method; establishing mathematical models among operation parameter, status input parameter and parameters such as boiler efficiency, NOx and the like in the combustion process to obtain a unit operation mode knowledge base; performing the whole plant energy-saving and emission-reducing comprehensive assessment and diagnosis; analyzing the unit operation potential; and providing a knowledge base and a rule for optimizing operation; optimizing boiler combustion parameter configuration aiming at different combustion indexes or an index combination to realize the optimization of multiple optimizing objects and propose an energy-saving and emission-reducing implementation scheme and measurement in a classification mode. The method not only can realize closed-loop optimizing control but also can realize on-line optimizing guide.
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