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1340 results about "Unit operation" patented technology

In chemical engineering and related fields, a unit operation is a basic step in a process. Unit operations involve a physical change or chemical transformation such as separation, crystallization, evaporation, filtration, polymerization, isomerization, and other reactions. For example, in milk processing, homogenization, pasteurization, and packaging are each unit operations which are connected to create the overall process. A process may require many unit operations to obtain the desired product from the starting materials, or feedstocks.

Manufacturing process for the production of polypeptides expressed in insect cell-lines

The present invention provides a manufacturing method for polypeptides that are produced in insect cells using a baculoviral expression system. In one example, the insect cell culture is supplemented with a lipid mixture immediately prior to infection (e.g., one hour prior to infection). The polypeptides are isolated from the insect cell culture using a method that employs anion exchange or mixed-mode chromatography early in the purification process. This process step is useful to remove insect-cell derived endoglycanases and proteases and thus reduces the loss of desired polypeptide due to enzymatic degradation. In another example, mixed-mode chromatography is combined with dye-ligand affinity chromatography in a continuous-flow manner to allow for rapid processing of the insect-cell culture liquid and capture of the polypeptide. In yet another example, a polypeptide is isolated from an insect cell culture liquid using a process that combines hollow fiber filtration, mixed-mode chromatography and dye-ligand affinity in a single unit operation producing a polypeptide solution that is essentially free of endoglycanase and proteolytic activities. In a further example, the isolated polypeptides are glycopeptides having an insect specific glycosylation pattern, which are optionally conjugated to a modifying group, such as a polymer (e.g., PEG) using a glycosyltransferase and a modified nucleotide sugar.
Owner:NOVO NORDISK AS

Full-temperature-range pressure swing adsorption gas separation, refinement and purification method

ActiveCN105749699AReduce energy consumptionBroaden the scope of adsorption separation applicationsSolidificationLiquefactionEnergy gradientPurification methods
The invention discloses a full-temperature-range pressure swing adsorption gas separation, refinement and purification method.By means of the difference of the temperatures and pressures of different raw material gases and the difference of the adsorption separation coefficients and physical chemistry properties of all components in the raw material gases in the temperature range of 80-200 DEG C and the pressure range of 0.03-4.0 MPa, the adsorption or desorption regeneration operation of the pressure swing adsorption circulation process is adjusted by coupling all separation methods, the adsorption theory that the pressure or temperature swing adsorption separation process is only limited to the adsorption and desorption regeneration circulation operation through pressure or temperature changes is expanded, and therefore all raw material gases are separated, refined and purified by achieving the energy gradient utilization in the gas separation, refinement and purification process and achieving the circulation operation, where adsorption, desorption and regeneration are easily matched and balanced, in the moderate to low cold and moderate to high temperature pressure swing adsorption separation process, and it is changed that a traditional adsorption method is only limited to the auxiliary effect of refinement and purification, and adsorption becomes the basic separation unit operation just as important as refinement, absorption and extraction separation.
Owner:SICHUAN TECHAIRS

Process for analyzing protein samples

Methods using gel electrophoresis and mass spectrometry for the rapid, quantitative analysis of proteins or protein function in mixtures of proteins derived from two or more samples in one unit operation are disclosed. In one embodiment the method includes (a) preparing an extract of proteins from each of at least two different samples; (b) providing a set of substantially chemically identical and differentially isotopically labeled protein reagents; (c) reacting the extract of proteins from different samples of step (a) with a different isotopically labeled reagent from the set of step (b) to provide two or more sets of isotopically differentially labeled proteins; (d) mixing each of the two or more sets of isotopically labeled proteins to form a single mixture of isotopically differentially labeled proteins; (e) electrophoresing the mixture of step (d) by an electrophoresing method capable of separating proteins within the mixture; (f) digesting at least a portion of one or more separated proteins of step (e) and (g) detecting the difference in the expression levels of the proteins in the two samples by mass spectrometry based on one or more peptides in the sample of labeled peptides. The analytical method can be used for qualitative and particularly for quantitative analysis of global protein expression profiles in cells and tissues, i.e. the quantitative analysis of proteomes.
Owner:DH TECH DEVMENT PTE +1

Optimized operation control method and system of distributed energy system

The invention discloses an optimized operation control method and system of a distributed energy system. The method include: S1, collecting environmental information and actual operation data of a unit so as to acquire a change rule of cold and hot load of a distributed energy station user with season and moment, and establishing a cold, hot and electric load prediction model; S2, optimizing the cold, hot and electric load prediction model on line by introducing real-time calibration factors and the actual operation data of the unit; S3, on the premise that the energy utilization efficiency is met, establishing a dynamic optimized load distribution model according to the dynamic requirements of the predicated cold, hot and electric load by taking a whole-plant economic benefit optimization as an objective, and outputting dynamic optimized load distribution results; S4, based on the whole-plant economic benefit optimization, establishing an optimal combination model according to the dynamic optimized load distribution results, and outputting a unit operation optimization command. High-precision load prediction information can be acquired, a corresponding optimization command is formed, and online optimization control is performed on the load dynamics and unit operation.
Owner:CHINA HUADIAN SCI & TECH INST

Nuclear power plant non-security-level DCS configuration testing method and system

The invention discloses a nuclear power plant non-security-level DCS configuration testing method and system comprising a virtual DCS platform and a nuclear power station simulating model coupled to the virtual DCS platform. The nuclear power station simulating model is used for, according to a nuclear power unit operation control instruction given by the virtual DCS platform, simulating the operation of a real nuclear power station, acquiring the operating data of the nuclear power unit, and returning the acquired data to the virtual DCS platform. The virtual DCS platform is used for, according to the operating data of the nuclear power unit, computing and analyzing the operating condition of the nuclear power unit in real time, evaluating a difference between an analyzed result and a designed operating condition, determining the correctness and the rationality of DCS configuration logic and parameters, giving corresponding logic modification and parameter optimization, and transplanting and applying a tested and modified result to the DCS configuration of a real nuclear power unit. The nuclear power plant non-security-level DCS configuration testing method and system may reduce the investment cost of the nuclear power station, shorten the construction period of nuclear power engineering, and decrease equipment loss.
Owner:中广核工程有限公司 +1
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