Serum-free medium for MDCK cell large-scale adherent culture and single-cell suspension culture

Abstract

The invention relates to the culture medium research and development technical field of modern biological technology and provides a serum-free medium for MDCK cell large-scale adherent culture and single-cell suspension culture, which comprises 21 amino acids, 6 vitamins, 8 salts, 8 lipids, 4 trace elements, 2 buffers, 1 protein hydrolysate, 1 acid-base indicator and 6 other additives. The serum-free medium can be prepared by the conventional preparation method, and an application method thereof is the conventional method. The serum-free medium has the beneficial effects that: the serum-free medium does not contain serum, has clear components, is beneficial for separating and purifying the product and improves the product quality; the serum-free medium supports long-term subculture of MDCK cells and does not require long-term and complex adaptation process; and the serum-free medium can well support the adherent growth and single-cell suspension growth of the MDCK cells, has clear components and easy preparation and utilization, and is suitable for mass production of biological products.

Description

【Technical field】 [0001] The invention relates to the technical field of medium research and development of modern biotechnology, in particular to a serum-free medium suitable for large-scale adherent culture of MDCK cells and single cell suspension culture. 【technical background】 [0002] Preventing viruses from endangering human beings is a matter of great concern to all countries. For many years, human beings have suffered huge losses every year due to viruses, such as rabies and bird flu. Rabies is caused by the rabies virus and is one of the most serious infectious diseases. Once the onset occurs, the mortality rate is very high, and so far there is no special and effective therapeutic drug except the vaccine for prevention. In addition, the harm caused by influenza virus is also very great. Every year, 5-15% of people are infected by seasonal influenza, 3-5 million severe cases, and up to 500,000 people die from it. What's more serious is that the current influenza v...

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