The invention provides a primer group and a probe group capable of simultaneously detecting various influenza viruses. Sequences of the primer group are shown as SEQ ID NO:1-8, and sequences of the probe group are shown as SEQ ID NO:9-12; and the influenza viruses include influenza A virus, influenza B virus, seasonal influenza virus H1 sub-type and H3 sub-type. On the basis, a kit, which is capable of simultaneously detecting the various influenza viruses, is provided. According to the prime group, the probe group and the kit, direct amplification and multiplex PCR are combined; direct RT-PCR can be conducted on suspected influenza cases or epidemiological investigation throat swab samples; and in comparison with a conventional method, a nucleic acid extraction step is avoided, so that it is conducive to implementation of automatic integrated detection and to improvement of efficiency. The prime group, the probe group and the kit have obvious advantages in aspect of being applied to rapid discrimination and detection of people infected by the influenza viruses, and the invention is simple and convenient to operate, short in detection time and relatively low in detection cost. The prime group, the probe group and the kit are applicable to rapid detection at a site of epidemic situation; and the prime group, the probe group and the kit have a broad and practical application value.