Kit for identifying subtypes of influenza A virus

An influenza A virus and kit technology, which is applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as high cost and complicated procedures, and achieve low cost, simple operation, and detection results. reliable results

Inactive Publication Date: 2010-11-03
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, microchips and flow cytometry methods are used for detection, but most of the procedures are complicated and expensive

Method used

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  • Kit for identifying subtypes of influenza A virus
  • Kit for identifying subtypes of influenza A virus
  • Kit for identifying subtypes of influenza A virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, identification of different subtypes of influenza A virus

[0046] The specific virus strains used in this experiment are as follows:

[0047] The virus strain of new type A H1N1 subtype of influenza A virus is A / Beijing / 501 / 2009 (H1N1) (swine influenza virus strain) in literature (Liu, B., Qin, C., Jiang, T., Hu, Y., Li, X., Zhang, X., Kang, X., Deng, Y., Zhang, Y., Li, Y., Liu, H., Jia, N., Chang, G, Zhao, H. ., Tong, Y., Qin, E., Zhu, Q. and Cao, W. Direct Submission Submitted (30-MAY-2009) Department of Virology, Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Beijing, Beijing 100071, Chin ); (provided by the Institute of Microbial Epidemiology, Academy of Military Medical Sciences of the Chinese People's Liberation Army);

[0048]The virus strain of influenza A virus type A H1N1 subtype (i.e. seasonal H1N1 subtype) is A / PR / 8 / 34 (H1N1) (seasonal influenza virus) in literature (Ryan Vander Veen, Kurt Kamrud, Mark Mogler, Alan T....

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Abstract

The invention discloses a kit for identifying subtypes of influenza A virus. The kit comprises the following primer pairs: one primer of which the sequence is shown in a sequence 5 in a sequence table and the other primer of which the sequence is shown in a sequence 6 in the sequence table; and the subtypes of influenza A virus are subtypes of new influenza A H1N1 virus, influenza H1N1 virus, influenza A H5N1 virus or influenza A H3N2 virus. Experiments prove that the method has the advantages of reliable detection results, high sensitivity (capable of detecting 103 copy number of the new influenza A H1N1 virus to the lowest), and good specificity; and particularly, the method can also separate the subtypes of the new influenza A H1N1 virus from the subtypes of common seasonal H1N1 influenza virus, which cannot be realized by the conventional technology. Besides, the method has the advantages of no need of precious equipment, simple and quick operation, and capacity of realizing high-flux quick detection. The kit and the PCR reagent have the advantages of high sensitivity, good specificity, wide sources and low cost. The invention provides a simple, feasible and effective method for early diagnosis of the infections of human influenza viruses.

Description

technical field [0001] The invention relates to a kit for identifying subtypes of influenza A virus. Background technique [0002] Influenza virus infection has always been a public health hazard that is difficult for humans to overcome. In March 2009, swine H1N1, a new type of swine influenza virus, spread from Mexico and spread widely around the world. It was an explosive epidemic and caused great losses to human beings. [0003] Influenza viruses mainly infect humans with types A and B, among which influenza A viruses are H1N1, H3N2, and H5N1 as common pathogenic subtypes. The highly pathogenic avian influenza virus discovered in recent years can cross the population barrier and infect humans. Different subtypes of influenza viruses have different virulence and transmission characteristics, which brings great difficulties to the diagnosis and treatment of infectious diseases. The rapid diagnosis of virus subtypes is the key to improving the efficacy of patients and con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/70C12Q1/68
Inventor 杨银辉林方熊伟康晓平李永强刘洪李裕昌常国辉祝庆余蔡绪禹孙婷婷
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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