41 results about "Highly pathogenic" patented technology

The invention discloses a multiplex fluorescent quantitative PCR (polymerase chain reaction) detection kit for prawn culture pathogenic bacteria, which is characterized in that the kit contains primers and probes for detecting the prawn culture pathogenic bacteria, wherein the primers and the probes are respectively used for detecting TDH-related hemolsin genes of vibrio parahaemolyticus, ORF107 genes of white spot syndrome viruses, small subunit ribosomal genes of shrimp enterocytozoon hepatopenaei, 37 kDa coat protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA (deoxyribonucleic acid) primer genes of full-eye iridovirus 1 and PirA [pi] genes of pathogens of bacterial prawns acute hepatopancreas necrosis; the method has the advantage that the detection of eight prawn culture pathogens can be realized at the same time. The method comprises the following steps of: obtaining a tcdB gene of a high-pathogenicity vibrio and a hemolsin gene of a vibrio harveyi;

The invention discloses a bacillus safensis strain and application thereof in aquaculture, and relates to the technical field of aquaculture disease prevention and control, the obtained bacillus safensis strain ZY-1 forms spores under a certain fermentation condition, can be prepared into powder for preservation, has a long shelf life, has the bacteriostatic activity retention rate of more than or equal to 85% after being stored in a dark place for 12 months at 4-25 DEG C, and has the antibacterial activity of more than or equal to 7.2%. The production, transportation and storage cost is saved, main bacteriostatic effect factors of the bacillus safensis ZY-1 strain exist in extracellular metabolites, the bacillus safensis ZY-1 strain is stable in performance and resistant to high temperature, acid and alkali, ultraviolet rays, protease and the like, and fermentation supernatant of the bacillus safensis ZY-1 strain can be directly applied or prepared into spray-dried powder and is suitable for various aquaculture application scenes such as pond splashing and feed mixing and feeding. The metabolite spray-dried powder has a remarkable killing effect on high-pathogenicity vibrios VpAHPND and VpTPD, the minimum inhibitory concentrations of metabolite spray-dried powder respectively reach 23.44-46.88 mu g / ml and 46.88-93.75 mu g / ml, and the metabolite spray-dried powder can be used for preventing and controlling prawn infectious hepatopancreas necrosis and prawn transparent larva diseases induced by the metabolite spray-dried powder and also has a certain killing function on pathogens of other aquatic animals.

This invention provides a highly pathogenic feline calicivirus strain, its vaccine, and its applications. The highly pathogenic feline calicivirus was isolated from pathogenic material and identified as a highly pathogenic strain, named FCV CC475, with its cDNA sequence shown in SEQ ID NO.1. This strain exhibits strong pathogenicity, causing infected animals to develop symptoms such as fever, paw pad ulcers, and dehydration by day 3 post-infection; the viral load after 5 generations is 10. 9.80 TCID 50 / ml. This strain produces high antibody titers after immunization of cats and rabbits, demonstrating good immunogenicity. Therefore, this highly pathogenic feline calicivirus has broad application prospects in the preparation of feline calicivirus infection vaccines, diagnostic reagents, or therapeutic drugs.

The high pathogenicity of the rift valley fever virus limits the research on the pathogenic mechanism and prevention, control and treatment products of the rift valley fever virus, the offspring replication-deficient virus particles are successfully saved through a reverse genetic manipulation technology, and the virus particles do not have the capacity of multiple rounds of infection on common cells; stable replication and passage can only be realized in a cell line for providing exogenous envelope glycoprotein; in addition, the virus particle carries an exogenous indication protein and can indicate cell infection. The Rift Valley fever virus-like particle provided by the invention can be used as a Rift Valley fever biosafety secondary laboratory research platform, the establishment of the virus particle greatly simplifies tedious procedures in the Rift Valley fever virus research process, and paves a way for screening of Rift Valley fever virus neutralizing antibodies, research and development of candidate vaccines and research of pathogenic mechanisms of the Rift Valley fever viruses.

This invention discloses a virulent strain of type II canine adenovirus, an inactivated vaccine, and their applications, belonging to the field of biotechnology. The virulent strain of type II canine adenovirus is named canine adenovirus type 2 Aa05 strain, with accession number CCTCC NO: V202605. The virus titer can reach 10. 6.0 TCID 50 / mL. This invention utilizes this strain to construct an animal model of canine adenovirus type II infection. This animal model exhibits typical clinical symptoms and can be used for future canine adenovirus vaccine development, immune protection assessment, and efficacy evaluation of preventive and therapeutic drugs. This invention also utilizes this strain to develop an inactivated vaccine with extremely high safety and immunogenicity. It can induce the body to produce high levels of specific neutralizing antibodies, which can protect immunized animals against infection with virulent CAV-2 strains and also against infection with highly pathogenic CAV-1 strains, demonstrating excellent cross-protection.

The invention belongs to the technical field of virus detection, and particularly relates to a primer composition for detecting an avian influenza NA gene, a detection method and a detection device. The avian influenza NA genes comprise NA genes of an influenza A virus H5N1, an influenza A virus H5N6, an influenza A virus H7N7, an influenza A virus H7N9 and an influenza A virus H9N2, and the primer composition comprises nucleotide sequences as shown in SEQ ID No.1-25. The primer composition for detecting the NA gene causing the highly pathogenic human and livestock co-infected avian influenza, provided by the invention, can be used for detecting whether a sample contains the NA gene causing the highly pathogenic human and livestock co-infected avian influenza, namely NA genes of influenza A virus subtypes H5N1, H5N6, H7N7, H7N9 and H9N2, and a result can be conveniently, quickly and accurately detected; in a practical application process, a detection result can be obtained within 40 minutes.

The invention relates to the field of crayfish evaluation, and discloses a method and a system for evaluating the WSSV resistance of procambarus clarkii, and the method comprises the following steps: sampling the procambarus clarkii, extracting target tissues from a sample, and extracting total RNA (Ribonucleic Acid) for accurate quantification of concentration and synthesis of cDNA (Complementary Deoxyribonucleic Acid); then TRIM23 gene quantitative detection is carried out on procambarus clarkii through a probe method, the purpose of calculating the Ct value is achieved, and finally the WSSV resistance of the procambarus clarkii is evaluated in combination with a relative quantitative mathematical model. According to the method, high pathogenicity or virus operation is avoided, the safety of the operation process is improved, the monitoring process is greatly shortened, and the accuracy is improved.

The present invention relates to a rapid, safe, and accurate method for preparing samples containing microbial or viral nucleic acids for amplification and for detecting the presence of highly pathogenic viruses such as Ebola. The method involves heating the sample in the presence of reagents including a solvent and a detergent. Reagents and compositions are also provided.

The invention discloses an SFTSV pseudovirus as well as a preparation method and application thereof. Based on the characteristics of high pathogenicity and transmissibility of the SFTSV, the virus infection animal experiment needs to be operated in a laboratory at the biosafety third level or above, so that the research and development of vaccines and therapeutic drugs are limited to a great extent. A novel SFTSV pseudovirus is obtained by deleting a nucleoprotein coding gene in an S gene of the SFTSV virus based on a reverse genetics technology, and the pseudovirus lacks a complete virus genome and replication ability and can be researched in a low-biosecurity-level laboratory; the pseudovirus system carries luciferase or a green fluorescent protein reporter gene, the virus load is quantified through the expression of the reporter gene, and the pseudovirus system can be used for evaluating the inhibition effect of a small-molecule inhibitor or a natural product on virus invasion; the pseudovirus can be used for simulating a natural infection process and evaluating a neutralizing antibody level induced by a vaccine. The invention provides a new development platform for discovery of anti-SFTSV drugs.

The application discloses a natural recombination novel duck reovirus variant (NDRV-GXNN-2025) strain, which has a preservation number of CCTCC No:V202613 and a preservation date of January 28, 2026. Researches show that the NDRV-GXNN-2025 strain has high pathogenicity to ducklings and goslings, and the mortality rates of 2-day-old ducklings and goslings are 30% and 50% respectively when the attack dose is 104.0 / 0.1ml; meanwhile, the strain has good immunogenicity and is an ideal vaccine candidate strain. Accordingly, the inventors also establish a preparation method of a corresponding inactivated vaccine. The novel duck reovirus inactivated vaccine prepared by the method has good stability and safety, can induce the body to produce high-level neutralizing antibodies after immunizing ducklings and goslings, and can effectively resist the attack of the novel duck reovirus variant, and has a good application prospect.

This invention discloses a feline calicivirus nucleic acid detection kit and detection method. The detection kit includes: (1) a nucleic acid enrichment module and (2) a TaqMan fluorescent PCR detection module. The beneficial effects are that the sensitivity is significantly improved, the operation is simplified, the functions are integrated, the pathogenicity is classified in one step (distinguishing between highly pathogenic strains and classic strains), the stability is excellent, and the specificity is strong.

This invention discloses an epitope peptide of the key SRCR5 domain of porcine reproductive and respiratory syndrome virus (PRRSV) CD163, an epitope peptide vaccine, and its applications. The epitope vaccine of this invention exhibits good safety, without safety issues such as virulence reversion, viremia, or recombination with other PRRSV strains. It also demonstrates good immunogenicity; 14 days after secondary immunization, it can induce pigs to produce specific antibodies against the CD163 SRCR5-FP14 epitope with a maximum agglutination titer of 1:16. Immunized pigs can resist challenge from the highly pathogenic PRRSV JAX1 strain. Since all PRRSV subtypes rely on interaction with the CD163 receptor to infect susceptible host cells, and the CD163 molecule is conserved, the epitope vaccine blocking PRRSV infection also provides broad-spectrum protection against multiple subtypes without affecting pig production performance. This novel epitope vaccine has broad potential applications in PRRS prevention and control.

The invention discloses high-pathogenicity mycoplasma bovis and application thereof, and belongs to the technical field of microorganisms. The mycoplasma bovis is mycoplasma bovis, the number of the mycoplasma bovis is MbST201NMG1, and the mycoplasma bovis is registered in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the mycoplasma bovis is CGMCC No: 45661. The mycoplasma bovis provided by the invention is high in minimum inhibition concentration to antibiotics, low in adhesion rate to Mac-T cells but high in invasion rate, and can be used for establishing a more typical animal clinical lesion model of the mycoplasma bovis compared with other mycoplasma bovis, so that a certain support is provided for subsequent research and development of vaccines.

The application provides an antibody or antibody binding fragment of high pathogenic porcine reproductive and respiratory syndrome virus ORF5 protein, the antibody has a relatively high relative affinity constant for PRRSV GP5 protein of different lineages and branches, can be combined with target protein with high sensitivity and high specificity, and has high stability; based on the antibody, the antibody can realize accurate, rapid and large-batch detection of PRRSV GP5 protein content in serum or related products, and has a good application prospect in PRRSV GP5 content detection.

This invention provides an attenuated vaccine against Coxsackievirus A6 (CVA6), its preparation method, and its application. By comparing a highly pathogenic clinical isolate (CVA6-HeB) with an attenuated strain (CVA6-TW141), the genetic basis of CVA6 virulence was systematically studied. This invention located the core virulence determinant to the P1 capsid region and identified a key lethal amino acid residue (VP3-238) that significantly weakens viral replication in target tissues. Based on this discovery, this invention designed and validated a candidate attenuated live vaccine with significantly reduced lethality.

The invention discloses a neutralizing antibody targeting a filovirus host receptor NPC1 and an antibody fusion protein of a broad-spectrum neutralized filovirus and a mutant thereof, the neutralizing antibody is composed of a specific heavy chain and a specific light chain, can specifically bind to an NPC1-C structural domain of the host receptor NPC1 protein, has a clear binding epitope, still has binding activity in an endosome acidic environment, and can be used for preparing a recombinant filovirus. The light chain of the antibody fusion protein constructed on the basis of the neutralizing antibody contains NPC2 protein and flexible Linker, the antibody fusion protein has broad-spectrum neutralizing activity on high-pathogenicity filoviruses and mutant strains thereof, and a new thought and a new strategy are provided for research and development of anti-filovirus infection drugs; good application prospects and industrial conversion values are realized.

The invention relates to the technical field of microorganisms, in particular to a highly pathogenic duck virus hepatitis III virus and an egg yolk antibody, preparation and application thereof. The virus strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 24, 2024, the preservation number is CGMCC No.46271, and the virus strain is named as RD240820B. The egg yolk antibody is prepared by using the highly pathogenic duck virus hepatitis III type virus strain as an antigen. The egg yolk antibody is applied to preparation of products for preventing or treating duck virus hepatitis III. In a virulence experiment on a duck embryo, the death rate of the virus strain reaches 80% after the duck embryo is inoculated for 36 hours, and the virus strain has strong pathogenicity. And the death rate within 60 hours after inoculation is 100%, and the strain has relatively strong pathogenicity. The death rate is still 60% when the diluent is 10-8, and the toxicity is strong. The ELD50 value of the strain is relatively low, which indicates that the strain has relatively strong pathogenicity to duck embryos. After the egg yolk antibody is injected, the protection rate reaches up to 97.6% after the diseased duck is treated, and the egg yolk antibody has a remarkable prevention and control effect.

The invention provides a polypeptide for detecting a high-pathogenicity Nephrin autoantibody, a kit and application of the polypeptide and the kit. The polypeptide for detecting the high-pathogenicity Nephrin autoantibody comprises one or two of polypeptide NP1 and polypeptide NP4, the amino acid sequence of the polypeptide NP1 is as shown in SEQ ID NO: 1, and the amino acid sequence of the polypeptide NP4 is as shown in SEQ ID NO: 4. Compared with an existing Nephrin autoantibody detection method, the method has the advantages that overnight incubation is not needed, and detection can be completed within one day; according to the detection method based on the magnetic beads, a high-flux instrument can be used for detection; moreover, the kit can be used for detecting IgG and IgM immune globulins of the Nephrin autoantibody, and has very important application value clinically.

The invention discloses a porcine reproductive and respiratory syndrome virus CD163 key SRCR5 structural domain epitope peptide, an epitope peptide vaccine and application. The epitope vaccine is good in safety, has no safety problems of virulence reversion, generation of viremia, recombination with other PRRSV strains and the like, has good immunogenicity, and can induce a pig to generate a specific antibody aiming at the CD163 SRCR5-FP14 epitope with the highest agglutination titer of 1: 16 14 days after secondary immunization, and the immunized pig can resist attack of a highly pathogenic PRRSV JAX1 strain. Each subtype strain of the PRRSV is dependent on interaction with a CD163 receptor so as to infect a host susceptible cell, and CD163 molecules are conservative, so that the epitope vaccine for blocking PRRSV infection also has a broad-spectrum protection effect on various subtype strains, the production performance of pigs is not influenced, and the novel epitope vaccine has a wide potential application prospect in PRRS prevention and control.

The invention discloses a specific primer group for simultaneously detecting 54 key pathogens as well as a related method and application thereof, the specific primer group is designed aiming at the 54 key pathogens, a target sequence is amplified by combining multiple PCR (Polymerase Chain Reaction) and a nanopore sequencing technology, and then purification, enrichment, library building and sequencing are carried out, so that the specific primer group can be used for simultaneously detecting the 54 key pathogens. A detection technology system capable of detecting 54 key pathogens at the same time is established, the method is rapid and accurate in detection and high in sensitivity, required reagents and instruments are portable, pathogen detection work can be carried out in a field environment, beneficial data support can be provided for source tracing of the pathogens, analysis of mutation sites can be carried out, and the detection efficiency is improved. The method has very prominent significance on early prevention and control of sudden new infectious diseases caused by high-pathogenicity pathogenic microorganisms.

The invention discloses a Lassa fever suckling mouse infection model as well as an establishment method and application thereof, belongs to the technical field of biology, and is suitable for a biosafety secondary laboratory. Aiming at the high pathogenicity and biosafety four-level laboratory operation limitation of the lassa virus, a recombinant virus for expressing lassa virus glycoprotein is constructed based on a vesicular stomatitis virus reverse genetics platform, and a lethal infection model is established in a 3-day-old BALB / c suckling mouse; and the dynamic invasion process after the recombinant virus infects the organism can be observed through fluorescence visualization. According to the model, the bio-safety risk of operating the wild type lasa virus in a high-grade bio-safety laboratory is avoided, so that the bio-safety pressure of the laboratory is remarkably reduced; a standardized intraperitoneal injection inoculation mode guarantees experiment repeatability and operation safety; the expensive cost of using high-grade biological safety facilities is avoided, and the method is suitable for research on lasa virus nerve invasion mechanism analysis, antiviral drug blood brain barrier penetration efficiency evaluation and the like.

A bacteria-based compound for the prevention and treatment of a number of strains of avian influenza via oral intake by way of feed or drinking water or both. The agent is an inventive treatment compound comprising a bacterial-based culture which is directed to the prevention and treatment of avian influenza primarily for use in commercial birds such as poultry. The inventive treatment compound disclosed herein is produced from a lipopolysaccharide of a Gram-negative bacterial strain of the Brevundimonas group. The compound may be delivered to poultry by way of liquid or dry feed according to an effective prescribed regimen. A similar treatment approach may be taken as well after the bird is infected. Use of the composition is effective in the prevention and treatment of a variety of avian influenza, including high pathogenicity avian influenza, low pathogenicity avian influenza, Newcastle disease virus, infectious bronchitis disease virus, and infectious laryngotracheitis virus.

The invention provides a primer composition and a kit for detecting high-pathogenicity zoonosis avian influenza virus and application, and belongs to the technical field of virus detection. The primer composition for detecting the highly pathogenic human-livestock avian influenza virus provided by the invention can be used for specifically detecting HA genes of H3N2 subtype, H5N6 subtype and H7N7 subtype of influenza A viruses, detection results can be obtained within 40 minutes, and the detection method is convenient, rapid and accurate. The loop-mediated isothermal amplification (LAMP) technology adopted by the invention has the characteristics of rapidness, high efficiency, low cost, high sensitivity, strong resistance and the like. The invention is a core link for critical and severe prevention, diagnosis and treatment and medication guidance of highly pathogenic influenza patients, and is a key technology for diagnosis of highly pathogenic human-livestock avian influenza.

The invention discloses a prawn culture pathogenic bacteria multiple ERA detection primer and probe based on a biochip technology and application of the prawn culture pathogenic bacteria multiple ERA detection primer and probe. The kit is characterized in that the primers and the probe respectively aim at tcdB genes of highly pathogenic vibrios, hypothetical protein genes of white spot syndrome viruses, 16S rRNA genes of enterocytozoon hepatopenaei, non-structural protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA primer enzyme genes of decapod iridovirus 1 and PirB genes of bacterial prawn acute hepatopancreatic necrosis pathogens; the method comprises the following steps of: detecting a capsid protein gene of a prawn infectious muscle necrosis virus, a capsid protein gene of a nedavirus, a capsid protein gene of the nedavirus, a structural protein ORF1 gene of the prawn infectious muscle necrosis virus, a non-structural polyprotein gene of a taura syndrome virus and a Rep-1b gene of a yellow head virus, and further discloses specific sequences. The sensitivity, the specificity and the accuracy are high.

The invention belongs to the technical field of specimen sampling tables. In order to solve the problems that an existing mobile high-pathogenicity respiratory infectious disease sample sampling table cannot effectively store sample test tubes and is not suitable for sampling bedridden people and other people who are inconvenient to move, the invention provides a mobile high-pathogenicity respiratory infectious disease sample sampling table which comprises a mobile sampling table body, the sample test tube conveying mechanism and the adjustable window are arranged on the movable sampling table body; specimen test tubes can be stably moved into the movable sampling table body through the specimen test tube conveying mechanism, effective storage of the specimen test tubes is achieved, and by adjusting the inclination angle of the adjustable window, the two hands of medical staff can get close to people who are inconvenient to move in bed and the like, so that sampling is facilitated.

The invention belongs to the technical field of molecular biology, and particularly relates to a preparation method and application of virions. According to the invention, a reverse genetic technology is utilized to construct a rift valley heat replication defective virus-like particle for visual tracking of virus infection dynamics. The virion is only subjected to amplification passage in NSm-Gn-Gc expression cells, only one round of infection occurs in other cells, amplification passage cannot be performed, the infectivity of the rift valley fever virus is reduced, and the operation difficulty and the exposure risk are reduced. According to the quantum dot-labeled particles, real-time visualization of infection dynamics is realized, and dynamic characteristics of virus invasion links are accurately quantified. The content of the invention can be expanded to animal models for tracking tissue organs that a single virion initially targets when invading a host in a non-replication state. A novel technical platform is established for exploring an infection replication mechanism of the rift valley fever virus, and an important reference is provided for tracking research of other high-pathogenicity viruses.

The invention provides a high-throughput screening method of an anti-Nipah virus drug. The method comprises the following steps: firstly, completing drug gradient dilution and planking pretreatment in a BSL-2 laboratory; then carrying out virus infection, cell culture and 72-hour incubation in the BSL-4 core region; the method comprises the following steps: carrying out rapid and low-cost visual preliminary screening on a whole-plate sample through crystal violet dyeing, and selectively carrying out TRIzol LS inactivation treatment on a small amount of potential effective sample supernatant; after the inactivated sample is transferred to a BSL-2 laboratory, the virus copy number is accurately quantified by adopting a qRT-PCR technology, and finally the drug inhibition ratio and the EC50 value are calculated. According to the method, through a staged screening strategy, on the premise of ensuring biological safety, the screening flux is greatly improved, the detection cost is reduced, and a reliable solution is provided for drug screening of high-pathogenicity pathogenic microorganisms.

The invention relates to the field of vaccine oil, and relates to white oil for poultry vaccine injection as well as a preparation method and application thereof. The preparation method comprises the following steps: 1, taking atmospheric and vacuum distillate oil of intermediate base crude oil and / or paraffin base crude oil as raw material oil, and contacting the raw material oil with an extraction agent for extraction treatment to obtain low aromatic oil; 2, hydrotreating the low aromatic hydrocarbon oil by using a hydrotreating catalyst to obtain hydrotreated generated oil; 3, carrying out hydrodewaxing treatment on the hydrotreated generated oil by adopting a hydrodewaxing catalyst to obtain hydrodewaxed generated oil; 4, performing primary hydrofining on the hydrodewaxing generated oil by adopting a primary hydrofining catalyst to obtain primary hydrofining generated oil; 5, carrying out secondary hydrofining on the primary hydrofining generated oil by adopting a secondary hydrofining catalyst to obtain secondary hydrofining generated oil; and 6, carrying out precise fractionation on the secondary hydrofining generated oil to obtain the white oil for poultry vaccine injection. The white oil antibody has excellent titer and is especially suitable for preparing highly pathogenic virus vaccines.

The present application relates to the field of bioengineering, and particularly to a production system and method for disposable high-pathogenic microorganism or inactivated vaccine. The method comprises: providing a self-definable assembly bio-culture module and a corresponding management module; the bio-culture module comprises: a bio-reaction device, an intermediate device and a purification device connected through detachable pipelines, and a liquid leakage sensing line is arranged on the pipelines; when the bio-culture module is started, a first set composed of readings on the liquid leakage sensing line is collected; the management module judges the risk state of the bio-culture module through the first set and controls the safe operation thereof. The present application provides a disposable production method and system suitable for high-pathogenic viruses, bacteria and inactivated systems, which has the advantages of flexible assembly, automatic leakage warning, sterilization and the like, and the cumbersome processes (such as centrifugation, cleaning and the like) in the fixed production method are omitted, thereby effectively saving the research and development time and cost.