25 results about "Highly pathogenic" patented technology

The invention discloses a bacillus safensis strain and application thereof in aquaculture, and relates to the technical field of aquaculture disease prevention and control, the obtained bacillus safensis strain ZY-1 forms spores under a certain fermentation condition, can be prepared into powder for preservation, has a long shelf life, has the bacteriostatic activity retention rate of more than or equal to 85% after being stored in a dark place for 12 months at 4-25 DEG C, and has the antibacterial activity of more than or equal to 7.2%. The production, transportation and storage cost is saved, main bacteriostatic effect factors of the bacillus safensis ZY-1 strain exist in extracellular metabolites, the bacillus safensis ZY-1 strain is stable in performance and resistant to high temperature, acid and alkali, ultraviolet rays, protease and the like, and fermentation supernatant of the bacillus safensis ZY-1 strain can be directly applied or prepared into spray-dried powder and is suitable for various aquaculture application scenes such as pond splashing and feed mixing and feeding. The metabolite spray-dried powder has a remarkable killing effect on high-pathogenicity vibrios VpAHPND and VpTPD, the minimum inhibitory concentrations of metabolite spray-dried powder respectively reach 23.44-46.88 mu g / ml and 46.88-93.75 mu g / ml, and the metabolite spray-dried powder can be used for preventing and controlling prawn infectious hepatopancreas necrosis and prawn transparent larva diseases induced by the metabolite spray-dried powder and also has a certain killing function on pathogens of other aquatic animals.

This invention provides a highly pathogenic feline calicivirus strain, its vaccine, and its applications. The highly pathogenic feline calicivirus was isolated from pathogenic material and identified as a highly pathogenic strain, named FCV CC475, with its cDNA sequence shown in SEQ ID NO.1. This strain exhibits strong pathogenicity, causing infected animals to develop symptoms such as fever, paw pad ulcers, and dehydration by day 3 post-infection; the viral load after 5 generations is 10. 9.80 TCID 50 / ml. This strain produces high antibody titers after immunization of cats and rabbits, demonstrating good immunogenicity. Therefore, this highly pathogenic feline calicivirus has broad application prospects in the preparation of feline calicivirus infection vaccines, diagnostic reagents, or therapeutic drugs.

The high pathogenicity of the rift valley fever virus limits the research on the pathogenic mechanism and prevention, control and treatment products of the rift valley fever virus, the offspring replication-deficient virus particles are successfully saved through a reverse genetic manipulation technology, and the virus particles do not have the capacity of multiple rounds of infection on common cells; stable replication and passage can only be realized in a cell line for providing exogenous envelope glycoprotein; in addition, the virus particle carries an exogenous indication protein and can indicate cell infection. The Rift Valley fever virus-like particle provided by the invention can be used as a Rift Valley fever biosafety secondary laboratory research platform, the establishment of the virus particle greatly simplifies tedious procedures in the Rift Valley fever virus research process, and paves a way for screening of Rift Valley fever virus neutralizing antibodies, research and development of candidate vaccines and research of pathogenic mechanisms of the Rift Valley fever viruses.

This invention discloses a virulent strain of type II canine adenovirus, an inactivated vaccine, and their applications, belonging to the field of biotechnology. The virulent strain of type II canine adenovirus is named canine adenovirus type 2 Aa05 strain, with accession number CCTCC NO: V202605. The virus titer can reach 10. 6.0 TCID 50 / mL. This invention utilizes this strain to construct an animal model of canine adenovirus type II infection. This animal model exhibits typical clinical symptoms and can be used for future canine adenovirus vaccine development, immune protection assessment, and efficacy evaluation of preventive and therapeutic drugs. This invention also utilizes this strain to develop an inactivated vaccine with extremely high safety and immunogenicity. It can induce the body to produce high levels of specific neutralizing antibodies, which can protect immunized animals against infection with virulent CAV-2 strains and also against infection with highly pathogenic CAV-1 strains, demonstrating excellent cross-protection.

The invention relates to the field of crayfish evaluation, and discloses a method and a system for evaluating the WSSV resistance of procambarus clarkii, and the method comprises the following steps: sampling the procambarus clarkii, extracting target tissues from a sample, and extracting total RNA (Ribonucleic Acid) for accurate quantification of concentration and synthesis of cDNA (Complementary Deoxyribonucleic Acid); then TRIM23 gene quantitative detection is carried out on procambarus clarkii through a probe method, the purpose of calculating the Ct value is achieved, and finally the WSSV resistance of the procambarus clarkii is evaluated in combination with a relative quantitative mathematical model. According to the method, high pathogenicity or virus operation is avoided, the safety of the operation process is improved, the monitoring process is greatly shortened, and the accuracy is improved.

The application discloses a natural recombination novel duck reovirus variant (NDRV-GXNN-2025) strain, which has a preservation number of CCTCC No:V202613 and a preservation date of January 28, 2026. Researches show that the NDRV-GXNN-2025 strain has high pathogenicity to ducklings and goslings, and the mortality rates of 2-day-old ducklings and goslings are 30% and 50% respectively when the attack dose is 104.0 / 0.1ml; meanwhile, the strain has good immunogenicity and is an ideal vaccine candidate strain. Accordingly, the inventors also establish a preparation method of a corresponding inactivated vaccine. The novel duck reovirus inactivated vaccine prepared by the method has good stability and safety, can induce the body to produce high-level neutralizing antibodies after immunizing ducklings and goslings, and can effectively resist the attack of the novel duck reovirus variant, and has a good application prospect.

This invention discloses a feline calicivirus nucleic acid detection kit and detection method. The detection kit includes: (1) a nucleic acid enrichment module and (2) a TaqMan fluorescent PCR detection module. The beneficial effects are that the sensitivity is significantly improved, the operation is simplified, the functions are integrated, the pathogenicity is classified in one step (distinguishing between highly pathogenic strains and classic strains), the stability is excellent, and the specificity is strong.

This invention discloses an epitope peptide of the key SRCR5 domain of porcine reproductive and respiratory syndrome virus (PRRSV) CD163, an epitope peptide vaccine, and its applications. The epitope vaccine of this invention exhibits good safety, without safety issues such as virulence reversion, viremia, or recombination with other PRRSV strains. It also demonstrates good immunogenicity; 14 days after secondary immunization, it can induce pigs to produce specific antibodies against the CD163 SRCR5-FP14 epitope with a maximum agglutination titer of 1:16. Immunized pigs can resist challenge from the highly pathogenic PRRSV JAX1 strain. Since all PRRSV subtypes rely on interaction with the CD163 receptor to infect susceptible host cells, and the CD163 molecule is conserved, the epitope vaccine blocking PRRSV infection also provides broad-spectrum protection against multiple subtypes without affecting pig production performance. This novel epitope vaccine has broad potential applications in PRRS prevention and control.

The invention discloses high-pathogenicity mycoplasma bovis and application thereof, and belongs to the technical field of microorganisms. The mycoplasma bovis is mycoplasma bovis, the number of the mycoplasma bovis is MbST201NMG1, and the mycoplasma bovis is registered in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the mycoplasma bovis is CGMCC No: 45661. The mycoplasma bovis provided by the invention is high in minimum inhibition concentration to antibiotics, low in adhesion rate to Mac-T cells but high in invasion rate, and can be used for establishing a more typical animal clinical lesion model of the mycoplasma bovis compared with other mycoplasma bovis, so that a certain support is provided for subsequent research and development of vaccines.

This invention provides an attenuated vaccine against Coxsackievirus A6 (CVA6), its preparation method, and its application. By comparing a highly pathogenic clinical isolate (CVA6-HeB) with an attenuated strain (CVA6-TW141), the genetic basis of CVA6 virulence was systematically studied. This invention located the core virulence determinant to the P1 capsid region and identified a key lethal amino acid residue (VP3-238) that significantly weakens viral replication in target tissues. Based on this discovery, this invention designed and validated a candidate attenuated live vaccine with significantly reduced lethality.

The invention discloses a neutralizing antibody targeting a filovirus host receptor NPC1 and an antibody fusion protein of a broad-spectrum neutralized filovirus and a mutant thereof, the neutralizing antibody is composed of a specific heavy chain and a specific light chain, can specifically bind to an NPC1-C structural domain of the host receptor NPC1 protein, has a clear binding epitope, still has binding activity in an endosome acidic environment, and can be used for preparing a recombinant filovirus. The light chain of the antibody fusion protein constructed on the basis of the neutralizing antibody contains NPC2 protein and flexible Linker, the antibody fusion protein has broad-spectrum neutralizing activity on high-pathogenicity filoviruses and mutant strains thereof, and a new thought and a new strategy are provided for research and development of anti-filovirus infection drugs; good application prospects and industrial conversion values are realized.

The invention relates to the technical field of microorganisms, in particular to a highly pathogenic duck virus hepatitis III virus and an egg yolk antibody, preparation and application thereof. The virus strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 24, 2024, the preservation number is CGMCC No.46271, and the virus strain is named as RD240820B. The egg yolk antibody is prepared by using the highly pathogenic duck virus hepatitis III type virus strain as an antigen. The egg yolk antibody is applied to preparation of products for preventing or treating duck virus hepatitis III. In a virulence experiment on a duck embryo, the death rate of the virus strain reaches 80% after the duck embryo is inoculated for 36 hours, and the virus strain has strong pathogenicity. And the death rate within 60 hours after inoculation is 100%, and the strain has relatively strong pathogenicity. The death rate is still 60% when the diluent is 10-8, and the toxicity is strong. The ELD50 value of the strain is relatively low, which indicates that the strain has relatively strong pathogenicity to duck embryos. After the egg yolk antibody is injected, the protection rate reaches up to 97.6% after the diseased duck is treated, and the egg yolk antibody has a remarkable prevention and control effect.

The invention provides a polypeptide for detecting a high-pathogenicity Nephrin autoantibody, a kit and application of the polypeptide and the kit. The polypeptide for detecting the high-pathogenicity Nephrin autoantibody comprises one or two of polypeptide NP1 and polypeptide NP4, the amino acid sequence of the polypeptide NP1 is as shown in SEQ ID NO: 1, and the amino acid sequence of the polypeptide NP4 is as shown in SEQ ID NO: 4. Compared with an existing Nephrin autoantibody detection method, the method has the advantages that overnight incubation is not needed, and detection can be completed within one day; according to the detection method based on the magnetic beads, a high-flux instrument can be used for detection; moreover, the kit can be used for detecting IgG and IgM immune globulins of the Nephrin autoantibody, and has very important application value clinically.

A bacteria-based compound for the prevention and treatment of a number of strains of avian influenza via oral intake by way of feed or drinking water or both. The agent is an inventive treatment compound comprising a bacterial-based culture which is directed to the prevention and treatment of avian influenza primarily for use in commercial birds such as poultry. The inventive treatment compound disclosed herein is produced from a lipopolysaccharide of a Gram-negative bacterial strain of the Brevundimonas group. The compound may be delivered to poultry by way of liquid or dry feed according to an effective prescribed regimen. A similar treatment approach may be taken as well after the bird is infected. Use of the composition is effective in the prevention and treatment of a variety of avian influenza, including high pathogenicity avian influenza, low pathogenicity avian influenza, Newcastle disease virus, infectious bronchitis disease virus, and infectious laryngotracheitis virus.

The invention discloses a prawn culture pathogenic bacteria multiple ERA detection primer and probe based on a biochip technology and application of the prawn culture pathogenic bacteria multiple ERA detection primer and probe. The kit is characterized in that the primers and the probe respectively aim at tcdB genes of highly pathogenic vibrios, hypothetical protein genes of white spot syndrome viruses, 16S rRNA genes of enterocytozoon hepatopenaei, non-structural protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA primer enzyme genes of decapod iridovirus 1 and PirB genes of bacterial prawn acute hepatopancreatic necrosis pathogens; the method comprises the following steps of: detecting a capsid protein gene of a prawn infectious muscle necrosis virus, a capsid protein gene of a nedavirus, a capsid protein gene of the nedavirus, a structural protein ORF1 gene of the prawn infectious muscle necrosis virus, a non-structural polyprotein gene of a taura syndrome virus and a Rep-1b gene of a yellow head virus, and further discloses specific sequences. The sensitivity, the specificity and the accuracy are high.

The invention belongs to the technical field of specimen sampling tables. In order to solve the problems that an existing mobile high-pathogenicity respiratory infectious disease sample sampling table cannot effectively store sample test tubes and is not suitable for sampling bedridden people and other people who are inconvenient to move, the invention provides a mobile high-pathogenicity respiratory infectious disease sample sampling table which comprises a mobile sampling table body, the sample test tube conveying mechanism and the adjustable window are arranged on the movable sampling table body; specimen test tubes can be stably moved into the movable sampling table body through the specimen test tube conveying mechanism, effective storage of the specimen test tubes is achieved, and by adjusting the inclination angle of the adjustable window, the two hands of medical staff can get close to people who are inconvenient to move in bed and the like, so that sampling is facilitated.

The invention belongs to the technical field of molecular biology, and particularly relates to a preparation method and application of virions. According to the invention, a reverse genetic technology is utilized to construct a rift valley heat replication defective virus-like particle for visual tracking of virus infection dynamics. The virion is only subjected to amplification passage in NSm-Gn-Gc expression cells, only one round of infection occurs in other cells, amplification passage cannot be performed, the infectivity of the rift valley fever virus is reduced, and the operation difficulty and the exposure risk are reduced. According to the quantum dot-labeled particles, real-time visualization of infection dynamics is realized, and dynamic characteristics of virus invasion links are accurately quantified. The content of the invention can be expanded to animal models for tracking tissue organs that a single virion initially targets when invading a host in a non-replication state. A novel technical platform is established for exploring an infection replication mechanism of the rift valley fever virus, and an important reference is provided for tracking research of other high-pathogenicity viruses.

The invention provides a high-throughput screening method of an anti-Nipah virus drug. The method comprises the following steps: firstly, completing drug gradient dilution and planking pretreatment in a BSL-2 laboratory; then carrying out virus infection, cell culture and 72-hour incubation in the BSL-4 core region; the method comprises the following steps: carrying out rapid and low-cost visual preliminary screening on a whole-plate sample through crystal violet dyeing, and selectively carrying out TRIzol LS inactivation treatment on a small amount of potential effective sample supernatant; after the inactivated sample is transferred to a BSL-2 laboratory, the virus copy number is accurately quantified by adopting a qRT-PCR technology, and finally the drug inhibition ratio and the EC50 value are calculated. According to the method, through a staged screening strategy, on the premise of ensuring biological safety, the screening flux is greatly improved, the detection cost is reduced, and a reliable solution is provided for drug screening of high-pathogenicity pathogenic microorganisms.

The invention relates to the field of vaccine oil, and relates to white oil for poultry vaccine injection as well as a preparation method and application thereof. The preparation method comprises the following steps: 1, taking atmospheric and vacuum distillate oil of intermediate base crude oil and / or paraffin base crude oil as raw material oil, and contacting the raw material oil with an extraction agent for extraction treatment to obtain low aromatic oil; 2, hydrotreating the low aromatic hydrocarbon oil by using a hydrotreating catalyst to obtain hydrotreated generated oil; 3, carrying out hydrodewaxing treatment on the hydrotreated generated oil by adopting a hydrodewaxing catalyst to obtain hydrodewaxed generated oil; 4, performing primary hydrofining on the hydrodewaxing generated oil by adopting a primary hydrofining catalyst to obtain primary hydrofining generated oil; 5, carrying out secondary hydrofining on the primary hydrofining generated oil by adopting a secondary hydrofining catalyst to obtain secondary hydrofining generated oil; and 6, carrying out precise fractionation on the secondary hydrofining generated oil to obtain the white oil for poultry vaccine injection. The white oil antibody has excellent titer and is especially suitable for preparing highly pathogenic virus vaccines.

The present application relates to the field of bioengineering, and particularly to a production system and method for disposable high-pathogenic microorganism or inactivated vaccine. The method comprises: providing a self-definable assembly bio-culture module and a corresponding management module; the bio-culture module comprises: a bio-reaction device, an intermediate device and a purification device connected through detachable pipelines, and a liquid leakage sensing line is arranged on the pipelines; when the bio-culture module is started, a first set composed of readings on the liquid leakage sensing line is collected; the management module judges the risk state of the bio-culture module through the first set and controls the safe operation thereof. The present application provides a disposable production method and system suitable for high-pathogenic viruses, bacteria and inactivated systems, which has the advantages of flexible assembly, automatic leakage warning, sterilization and the like, and the cumbersome processes (such as centrifugation, cleaning and the like) in the fixed production method are omitted, thereby effectively saving the research and development time and cost.

This invention provides a systemic feline calicivirus (FCV) yolk antibody, its preparation method, and its application. The yolk antibody is prepared using a novel, highly pathogenic FCV strain, CC475, whose cDNA sequence is shown in SEQ ID NO.1. This strain belongs to the VS-FCV strain, which causes fatal systemic disease in cats. It has low homology with classic feline caliciviruses. The yolk antibody prepared using this VS-FCV strain exhibits high titer, good stability, and rapid efficacy, and can be used for rapid treatment or emergency prevention of systemic feline calicivirus infection with a high protection rate.

The present application relates to the field of virus detection technology and animal epidemic disease molecular detection technology, and particularly relates to a primer probe combination and kit for detecting avian influenza virus, H5 subtype, Gamma genus and Delta genus avian coronavirus, the primer probe combination is designed based on the M gene fragment and H5 subtype HA gene fragment of avian influenza virus and the conserved region of avian coronavirus RdRp gene, and includes specific primers and corresponding fluorescent probes for universal avian influenza virus, H5 highly pathogenic subtype, avian coronavirus Gamma subtype and Delta subtype respectively, and the four targets can be simultaneously amplified and distinguished in the same reaction system.

This invention discloses a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus, belonging to the field of rapid detection kit technology. It includes a housing, protective components, and a sample collection mechanism. The back of the housing is symmetrically fitted with hinges via bolts. This triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus, through its primer, fluorescent probe, and reagent carrier components and sample collection mechanism, enables the rapid screening of three highly pathogenic canine viruses (canine distemper virus CDV, canine coronavirus CCV, and canine parvovirus CPV) using a lyophilization process based on quantitative real-time PCR technology.

The invention relates to a primer probe group and a kit for specific detection of a highly pathogenic monkey pox virus subtype. The preferable combination of the primer probe group is as follows: a forward primer: SEQ ID NO: 3, a reverse primer: SEQ ID NO: 4 and a probe: SEQ ID NO: 5. The primer probe group and the kit disclosed by the invention have specific amplification capacity on the monkey pox virus Ib type and do not have cross reaction on non-monkey pox virus Ib type.

This invention proposes a screening method for biocontrol Trichoderma strains based on the dual criteria of oomycete parasitism and lethality. Belonging to the field of agricultural biotechnology, this invention addresses the technical problem that existing screening techniques often rely solely on the growth inhibition rate of confrontation cultures, making it difficult to distinguish between the antibacterial and lethal effects of biocontrol bacteria. This invention establishes a progressive screening system. The method uses highly pathogenic oomycetes, namely *Phytophthora capsici* (… Phytophthora capsici ) and ultimate pyrophyllium ( Globisporangium ultimum Using oomycetes as the target, and based on the determination of growth inhibition rate through confrontation culture, this invention creatively introduces a step of "pathogen re-isolation and detection of activity based on oomycete selective culture medium" combined with microscopic observation of hyperparasitism. This method effectively eliminates false-positive strains, significantly improves the accuracy of biocontrol strain screening and field efficacy, and is suitable for large-scale exploration of biocontrol resources.