Kit for detecting multiple influenza viruses by polymerase chain reaction (PCR) microarray

An influenza virus and microarray technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, fluorescence/phosphorescence, etc., can solve problems such as antibody-specific interference, and achieve the goal of reducing pollution, workload, and working time. Effect

Active Publication Date: 2012-02-01
DAAN GENE CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the experimental process is often interfered by non-specific agglutination factors, antibody specificity, etc., and more stringent requirements are put forward for the standardization of antigens and the preparation technology of monoclonal antibodies.

Method used

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  • Kit for detecting multiple influenza viruses by polymerase chain reaction (PCR) microarray
  • Kit for detecting multiple influenza viruses by polymerase chain reaction (PCR) microarray
  • Kit for detecting multiple influenza viruses by polymerase chain reaction (PCR) microarray

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 PCR Microarray Detects Various Influenza Virus Nucleic Acid Kits and Its Use

[0038] 1. Prepare a kit including the following components: 1 piece of 96-well PCR microarray reaction plate, 2 tubes of PCR reaction solution, 900 μl / tube.

[0039] 2. Collection, preservation and transportation of specimens:

[0040] 2.1 Applicable specimen types: nasal swab, throat swab, nasopharyngeal aspirate

[0041] 2.2 Specimen collection, storage and delivery:

[0042] It should be collected as soon as possible after the onset of the disease. The specific operation method is as follows: use a sterilized cotton swab to take nasal or throat secretions, and immediately put them into a small test tube containing 2 mL of sterilized normal saline or Eagle solution or 0.5% hydrolyzed milk protein Hanks solution. Label it well, put it in an ice bottle and carry it to the laboratory or freeze it at low temperature (-20℃~-70℃). Dry ice is used for long-distance transport of specim...

Embodiment 2

[0051] Example 2 Application of PCR Microarray to Detect Various Influenza Virus Nucleic Acid Kits to Detect Clinical Samples

[0052] Select 8 cases of throat swab specimens tested as negative for influenza virus by serological methods, and 9 cases as positive for corresponding influenza virus by serological methods, nucleic acid extraction, PCR amplification and result analysis are carried out with reference to Example 1, and negative, Detection of positive quality controls.

[0053] Interpretation of the test results: the amplification curves of the negative quality control products of each influenza virus are irregular curves or have no CT value; The amplification of the corresponding influenza virus nucleic acid was detected, and the test results of the negative samples were all negative (see attached Figure 3-Figure 12 ).

[0054] The detection results of 17 specimens in this test were completely consistent with the ELISA identification results, indicating that it is ...

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Abstract

The invention relates to a kit for detecting multiple influenza viruses by a polymerase chain reaction (PCR) microarray. The kit comprises mainly a PCR microarray reaction plate, a PCR reaction solution, and a packaging box for centralized and partitioned packaging of reagent bottles or reagent tubes. Through the combination of a real-time fluorescence PCR technology and a microarray technology, on the single PCR microarray reaction plate, the kit can detect eight influenza viruses comprising influenza A virus, influenza B virus, seasonal influenza H1 subtype virus, seasonal influenza H3 subtype virus, influenza A H1N1 virus, highly pathogenic avian influenza H5 subtype virus, highly pathogenic avian influenza H7 subtype virus, and highly pathogenic avian influenza H9 subtype virus simultaneously. A result of detection adopting the kit can be utilized for differential diagnosis and monitoring of pathogens capable of causing influenza.

Description

technical field [0001] The invention relates to a PCR microarray detection kit for multiple influenza viruses. The kit uses real-time fluorescent PCR technology combined with a microarray method to simultaneously detect multiple influenza virus nucleic acids on the same reaction plate. Background technique [0002] Influenza virus, referred to as influenza virus, is an RNA virus that causes influenza in humans and animals, and belongs to the Orthomyxoviridae family. The target of influenza virus invasion is the epithelial cells of the respiratory tract mucosa. After infection, it will cause acute upper respiratory tract infection. Influenza viruses can spread through the air and cause large-scale epidemics, especially in people with weakened immunity, which can cause severe symptoms such as pneumonia and heart failure. The main clinical features are sudden onset, high fever, cough, dyspnea, exhaustion, high morbidity, and low mortality. [0003] According to the objects in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64C12N15/11
Inventor 李明陈华云高秀洁
Owner DAAN GENE CO LTD
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