258 results about "Influenza B viruses" patented technology

An influenza vaccine adjuvanted with a sub-micron oil-in-water emulsion elicits significantly higher immune responses in human pediatric populations. Compared to an existing unadjuvanted pediatric influenza vaccine, the adjuvanted vaccines provided herein can induce in children a longer persistence of high serum antibody titers and also longer seroconversion and seroprotection. The improvement in immune responses is seen for both influenza A virus and influenza B virus strains, but it is particularly marked for influenza B virus. Moreover, while the existing vaccine provides poor immunity in children after a single dose, the adjuvanted vaccine provides high seroprotection rates against the influenza A virus H3N2 subtype even after a single dose. Furthermore, the adjuvanted vaccine offers significantly better seroprotection against mismatched strains of influenza A virus.

The present invention relates inter alia to the treatment or prevention of influenza virus infection (including subtypes influenza A virus, influenza B virus, avian strain H5N1, A / H1N1, H3N2 and / or pandemic influenza) using compounds which inhibit the activity of p59-HCK and to a method of screening for a candidate drug substance intended to prevent or treat influenza virus infection in a subject, said method comprising identifying a test substance capable of inhibiting p59-HCK activity.

Provided is a human antibody having a neutralization activity against a human influenza virus. More specifically, provided is a human antibody which recognizes a highly conserved region in a human influenza A virus subtype H3N2 or a human influenza B virus and has a neutralization activity against the virus. The human antibody is a human anti-human influenza virus antibody, which has a neutralization activity against a human influenza A virus subtype H3N2 and binds to a hemagglutinin HA1 region of the human influenza A virus subtype H3N2, or which has a neutralization activity against a human influenza B virus, and includes, as a base sequence of a DNA encoding a variable region of the antibody, a sequence set forth in any one of SEQ ID NOS: 5 to 12.

The invention relates to a novel application of patchouli alcohol or cabline patchouli CO2 supercritical extract or cabline patchouli oil in the field of pharmaceutical. The cabline patchouli CO2 supercritical extract or cabline patchouli oil contains patchouli alcohol. The patchouli alcohol has obvious effects of inhibiting and killing influenza A1 virus, influenza B virus and birds influenza virus H5N1.

The invention discloses a respiratory pathogen multi-detection reagent kit. The respiratory pathogen multi-detection reagent kit has the advantages that the respiratory pathogen multi-detection reagent kit is based on multi-PCR (polymerase chain reaction) technologies, detection results can be determined by the aid of fluorescence resonance energy transfer via the melting temperature ranges, the respiratory pathogen multi-detection reagent kit can be used for qualitatively simultaneously detecting 16 types of respiratory pathogens, the 16 types of respiratory pathogens include 12 types of RNA(ribonucleic acid) viruses (influenza A viruses, influenza B viruses, H1N1 influenza A viruses, type A and type B respiratory syncytial viruses, type -1 / -2 / -3 parainfluenza viruses, type OC43 coronaviruses, type 229E coronaviruses, rhinoviruses and human metapneumovirus), 2 types of DNA (deoxyribonucleic acid) viruses (adenoviruses and bocavirus) and 2 types of bacteria (mycoplasma pneumoniae andbordetella pertussis), the respiratory pathogen multi-detection reagent kit is high in detection sensitivity, and the sensitivity even can reach 1 copy / reaction; the multi-detection reagent kit is good in specificity, and negative results of pathogens which have identical sampling sites and similar pathogenic mechanisms and are not in the detection range of the respiratory pathogen multi-detectionreagent kit can be obtained; the respiratory pathogen multi-detection reagent kit is short in operation time and easy to operate and can be used for quickly detecting the 16 types of respiratory pathogens in a single tube of a reaction system, the results are clear and are easy to interpret, and the like.

The invention provides a polycyclic pyridone compound as well as pharmaceutical composition and an application thereof. Specifically, the polycyclic pyridone compound is a compound shown as the formula (I) or pharmaceutically acceptable salt, solvate or hydrate of the compound. The compound can be used for preparing drugs for preventing or treating infectious diseases of mammals, and is particularly used for preparing drugs for preventing, treating, relieving and / or treating orthomyxovirus infection such as influenza A virus, influenza B virus and influenza C virus.

Described are binding molecules, such as human monoclonal antibodies, that bind to hemagglutinin of influenza B viruses, and have a broad neutralizing activity against such influenza viruses. These binding molecules do not bind to hemagglutinin of influenza A viruses. Further provided are nucleic acid molecules encoding the binding molecules, and compositions comprising the binding molecules. The binding molecules can be used in the diagnosis of, prophylaxis against, and / or treatment of influenza B virus infections.

The invention discloses a method for simultaneously detecting twelve kinds of common respiratory viruses. According to the method, primers and probes are designed according to gene conservative areas of the twelve kinds of common respiratory viruses, namely influenza A virus, influenza B virus, influenza C virus, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, rhinovirus, Bocavirus, adenovirus, coronavirus, metapneumovirus and respiratory syncytial virus, nucleic acid fragments of samples to be measured are extracted for amplifying, and finally, the samples are separated by using a capillary electrophoresis method. The method disclosed by the invention has the advantages of low required sample size, high sensitivity and accuracy, good specificity and low cost; the defects that the conventional single tube multiplex fluorescence PCR (Polymerase Chain Reaction) detection primers are difficult to design, and multicolor fluorescence mutually intervenes and is not easy to part are overcome, the defects that a chip detection method is tedious in operation, high in detection cost and the like are also overcome, and a new method is provided for screening the respiratory viruses.

The present invention relates to methods of detection, as well as assays, reagents and kits for the specific detection of 15 clinically important respiratory viruses including influenza A and B viruses, human respiratory syncytial viruses, human metapneumoviruses, human enteroviruses, all serotypes of rhinoviruses, 7 serotypes of adenoviruses, parainfluenza viruses types 1, 2, 3, and 4, as well as coronaviruses NL, 229E, OC43, and SARS-CoV. The present invention allows for the detection of each of these respiratory viruses in a single assay.

The invention provides viscose fiber containing a radix isatidis extract. According to the viscose fiber, the content of radix isatidis microcapsules is 9.1%-9.79%, the dry fracture strength of the fiber is 2.70cN / dtex-2.75cN / dtex, the wet fracture strength of the fiber is 1.70cN / dtex-1.75cN / dtex, the elongation at break of the fiber is 22%-23%, the antibacterial rate of the fiber to staphylococcus aureus is 99.0%-99.8%, the antibacterial rate of the fiber to Candida albicans is 96.6-98.8%, the antibacterial rate of the fiber to typhoid bacillus is 97.2%-99.1%, and the fiber has very strong effects of inhibiting and killing influenza A virus and influenza B virus. The invention further provides a preparation method of the viscose fiber containing the radix isatidis extract. The preparation method comprises a step of modifying the radix isatidis microcapsules. According to the preparation method, the loss of the radix isatidis microcapsules in the preparation process of the fiber is reduced, the dismantling backwashing rate of a coagulating bath filter is reduced, and the distribution uniformity of the radix isatidis microcapsules in the fiber is increased.

The invention relates to the field of molecular biology detection, in particular to detection of novel coronavirus, influenza A virus and influenza B virus. The invention provides a composition for detecting the viruses, and meanwhile, the invention further provides a kit containing the composition, application of the composition and a method for detecting and typing the viruses causing respiratory tract infection. The composition is combined with a fluorescent probe method, three viruses causing respiratory tract infection can be detected and typed at the same time in one tube, and the composition, the kit and the method have the advantages of being low in cost, high in flux, short in consumed time, easy and convenient to operate and capable of avoiding false positive caused by cross among samples and environmental pollution.

The present invention encompasses methods of producing influenza B viruses in cell culture. The influenza B viruses may have desirable characteristics, such as enhanced replication in eggs and may be used, for example, in vaccines and in methods of treatment to protect against influenza B virus infection.

The present disclosure relates to binding molecules, such as human monoclonal antibodies, that bind to an epitope in the stem region of hemagglutinin of influenza A viruses of phylogenetic group 1 and group 2, as well as influenza B viruses, and have a broad neutralizing activity against such influenza viruses. The disclosure provides nucleic acid molecules encoding the binding molecules, their sequences and compositions comprising the binding molecules. The binding molecules can be used in the diagnosis, prophylaxis and / or treatment of influenza A viruses of phylogenetic groups 1 and 2, as well as influenza B viruses.

The invention discloses combined detection test paper of influenza A virus antigen and influenza B virus antigen and a preparation method thereof. The test paper comprises a sample pad, a fiberglass membrane containing a colloidal gold particle label, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane comprises a detection area which is coated with an influenza A virus antibody, a detection area which is coated with an influenza B virus antibody and a control area which is coated with an goat anti-rabbit antibody; the colloidal gold particle label comprises a micro signal amplification system and a colloidal gold labeled rabbit IgG antibody; and the micro signal amplification system is a colloidal gold particle-avidin-biotin-influenza A / B virus antibody. According to the invention, an avidin-biotin microsignal amplification system is added in a double-antibody sandwich detection system, the signal of a target antibody is enlarged, the detection sensitivity is increased, false negative or detection omission due to weak signals can be avoided, simultaneously combined detection can be carried out on the influenza A and B virus antigens, and the detection time, sample and cost can be saved.

The invention provides sarcandra glabra high-efficiency antibacterial antiviral cellulosic fiber. The fiber is prepared from chitosan-functional ingredient compound particles and silicic acid compound colloid, wherein the content of the chitosan-functional ingredient compound particles is 9.5 to 9.7%, and the content of the silicic acid compound colloid is 2 to 3.5%. The invention further provides a preparation method of the sarcandra glabra high-efficiency antibacterial antiviral cellulosic fiber. The preparation method comprises the steps of preparing the chitosan-functional ingredient compound particles, preparing the silicic acid compound colloid, blending, and spinning and molding. In a preparation process of the fiber disclosed by the invention, running off or loss of functional ingredients is reduced; according to the prepared fiber, the functional ingredients are distributed on fiber cortex and surface portions; according to the prepared fiber, the functional ingredients which are embedded in the fiber cortex or surface in a particle shape are firmly combined with the fiber, so that loss is small in a washing and friction process; the fiber disclosed by the invention can effectively resist influenza A virus and influenza B virus, and also has higher inhibition ratios on staphylococcus aureus, candida albicans and typhoid bacillus.

The invention relates to the field of medical detection, in particular to a primer probe combination for detecting six respiratory viruses, a kit and application. According to the kit provided by theinvention, a method of combining isothermal amplification and a micro-fluidic chip is adopted; the kit can accurately detect novel coronavirus (2019-nCoV) S and N target genes and influenza A virus, novel influenza A H1N1 virus (2009), influenza A H3N2 virus, influenza B virus and respiratory syncytial virus at the same time, and the purpose of distinguishing the novel coronavirus from common influenza virus is achieved. The kit disclosed by the invention has the advantages of multiple detection indexes, simplicity and convenience in operation, short detection time and the like, six virus indexes can be simultaneously detected by one-time sample adding, the time from sample treatment to report is within 1.5 hours, and the kit is more time-saving and labor-saving than qPCR method.

Disclosed are methods and compositions useful in identifying inhibitors of influenza virus, such as influenza A and B virus. Also disclosed are methods for preparing compositions for administration to animals, including humans infected with or to protect against influenza virus.

The invention belongs to the field of biotechnology application and relates to a multiple fluorescence RT-PCR method with internal quality control for simultaneously detecting influenza A virus, influenza B virus and influenza C virus, and a kit. Specific primers and probes are designed for conserved sequences of an M gene, an NS gene and an NP gene of representative strains of the influenza A virus, the influenza B virus and the influenza C virus. The one-step multiple fluorescence RT-PCR method with internal quality control is established. The method is simple and convenient in operation, overcomes tediousness of single detection in single hole of conventional fluorescence RT-PCR detection, simplifies operation processes, and saves the testing cost. The method and the kit provide strong technical support for virus on-site detection, hygienic evaluation of food and water, clinical diagnosis, and the like by utilization of high specificity, high sensitivity, high efficiency and high stability of the method and the kit.

The present disclosure relates to a real-time fluorescence multiplex PCR primer probe for seven common respiratory system influenza virus pathogens and a kit. Seven influenza viruses are influenza A virus, influenza B virus, parainfluenza virus type I, parainfluenza virus type II, parainfluenza virus type III, respiratory syncytial virus and adenovirus. The present disclosure also provides a kit for multiplex fluorescence quantitative PCR detection of the seven influenza viruses, and the kit includes the primer probe set. The kit significantly improves the sensitivity, specificity, and simplicity of detection of the common respiratory system pathogens.

The invention relates to the field of molecular biology detection, and particularly relates to detection of novel coronavirus 2019- nCoV, influenza A virus, influenza B virus, respiratory adenovirus,respiratory syncytial virus and mycoplasma pneumoniae. The invention provides a composition for detecting the pathogens. Meanwhile, the invention further provides a kit containing the composition, application of the composition and a method for detecting and typing the pathogens causing respiratory tract infection. The composition is combined with a fluorescent probe method, six pathogens causingrespiratory tract infection can be detected and typed at the same time in two tubes, and the advantages that the cost is low, the flux is high, the influence of interference substances is avoided, operation is easy and convenient, and false positive and environmental pollution caused by sample crossing are avoided are achieved.

The invention belongs to the field of molecular biological detection, and relates to an RPA (recombinase polymerase amplification) primer and an RPA probe for detecting influenza B viruses, a kit andapplication of the RPA primer. The sequences of upstream and downstream primers are B-F and B-R, and the sequence of the probe is represented by SEQ ID. NO. 2. The RPA primer and the RPA probe for detecting the influenza B viruses can be used for rapidly detecting the influenza B viruses or simultaneously detecting influenza A and B viruses; the RPA downstream primer for the influenza B viruses can serve as a PCR amplification primer for the influenza B viruses. When the RPA primer and the RPA probe are used for detecting the influenza B viruses, the detection time is short, sensitivity and specificity are high, and the influenza A and B viruses can be simultaneously detected rapidly, accurately and specifically.

The invention discloses pseudovirions; a capsid protein of MS2 bacteriophage is wrapped with a DNA-protein complex of a detection sequence formed by series connection of three base fragments of influenza A virus, influenza B virus and beta-actin successively. The invention also provides a preparation method of the pseudovirions and an application of the pseudovirions as quality control products for detecting the influenza A virus and the hepatitis B virus. The preparation method of the pseudovirions is simple and is easy to operate; the obtained pseudovirus is high in purity, can be used as the quality control products in an influenza A virus and influenza B virus nucleic acid detection kit, and has the characteristics of no infectivity, good stability, ribonuclease resistance and the like.

Belonging to the molecular biology field, the invention relates to a primer set for respiratory tract infection pathogen detection, a rapid diagnostic kit and a detection method. The primer set can achieve one-time detection of the following 9 respiratory tract infection pathogens: influenza A virus, influenza B virus, respiratory syncytial virus, parainfluenza virus type I, parainfluenza virus type II and parainfluenza virus type III, adenovirus, mycoplasma pneumonia, legionella pneumonia, chlamydia pneumonia and human rhinovirus. The invention adopts solid phase recombinase-polymerase constant temperature gene amplification method to detect respiratory multiple infection pathogens. The primer set adopted by the invention for respiratory tract infection pathogen detection has strong specificity and high amplification efficiency, can effectively and rapidly detect the 9 pathogens simultaneously, and realizes multiplex detection.

The present invention relates inter alia to the treatment or prevention of influenza virus infection (including subtypes influenza A virus, influenza B virus, avian strain H5N1, A / H1N1, H3N2 and / or pandemic influenza) using compounds which inhibit the activity of p59-HCK and to a method of screening for a candidate drug substance intended to prevent or treat influenza virus infection in a subject, said method comprising identifying a test substance capable of inhibiting p59-HCK activity.

The invention relates to a triazine compound with a structure represented by a formula A, or a stereoisomer, a prodrug, an active metabolite or a pharmaceutically acceptable salt, a solvate or a crystal form thereof, a pharmaceutical composition of the triazine compound, the stereoisomer, the prodrug, the active metabolite or the pharmaceutically acceptable salt, the solvate or the crystal form, and a use method of the triazine compound. In addition, the invention also relates to a method for preparing a 3C-like cysteine protease inhibitor or a medicine for treating and / or preventing virus infectious diseases. In particular, the present invention relates to the use for treating viral infectious diseases such as Middle East Syndrome Related Coronavirus (MERS-CoV), Severe Acute Respiratory Syndrome Related Coronavirus (SARS-CoV), Influenza A virus, Influenza B virus, Novel Coronavirus Pneumonia (COVID-19) and the like.

Described are binding molecules, such as human monoclonal antibodies, that bind to hemagglutinin of influenza B viruses, and have a broad neutralizing activity against such influenza viruses. These binding molecules do not bind to hemagglutinin of influenza A viruses. Further provided are nucleic acid molecules encoding the binding molecules, and compositions comprising the binding molecules. The binding molecules can be used in the diagnosis of, prophylaxis against, and / or treatment of influenza B virus infections.

The invention discloses a kit for simultaneously detecting nucleic acids of seven respiratory pathogens and application of the kit. The kit is based on a double amplification technology (RNA isothermal amplification and multi-biotin signal amplification), and can detect H1N1 / H3N2 type influenza A viruses, influenza B viruses, respiratory syncytial viruses, 1 / 2 / 3 type human parainfluenza viruses, B / E type adenoviruses, mycoplasma pneumoniae and chlamydia pneumoniae. The kit of the invention does not need RNA extraction, and is not prone to pollution, high in sensitivity and good in specificityin the process of detection, and can be widely applied to detection of the nucleic acids of the above seven respiratory pathogens.

The invention discloses a respiratory tract infection pathogen nucleic acid combined detection kit. The invention develops a primer and probe combination for detecting various respiratory tract infection pathogens such as novel corona virus, influenza A virus, influenza B virus, respiratory syncytial virus, human parainfluenza virus, adenovirus, mycoplasma pneumoniae and chlamydia pneumoniae by combining a multiple fluorescent quantitative PCR technology and a diversion hybridization gene chip technology. The nucleotide sequences of the primer and probe combination are shown in SEQ ID NO: 1-36in sequence. The respiratory tract infection pathogen nucleic acid combined detection kit is constructed. Synchronous joint detection of eight respiratory tract infection pathogens can be realized, the detection accuracy is good, the specificity is strong, the sensitivity is high, the repeatability is good, false negative and false positive are low, the detection time is short, the cost is low, apatient can be comprehensively detected, the pathogens can be accurately positioned, timely treatment is carried out or corresponding isolation measures are carried out, and the kit has important significance for effectively controlling respiratory tract infection to prevent related infectious infection outbreak.

The invention provides a nucleic acid dual fluorescence PCR (Polymerase Chain Reaction) detection kit for an influenza A / B virus. The detection kit comprises an RNA (Ribonucleic Acid) enzyme inhibitor, an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) liquid, an enzyme mixed liquor, an influenza A / B virus dual reaction liquid, a positive control and a negative control, wherein the influenza A / B virus dual reaction liquid comprises a component (1) which consists of a pair of primers for detecting the influenza A virus and a probe for detecting the influenza A virus and a component (2) which consists of a pair of primers for detecting the influenza B virus and a probe for detecting the influenza B virus; and the enzyme mixed liquor comprises a Taq enzyme and a reverse transcription enzyme. By adopting the detection kit, the influenza A and B viruses can be detected at the same time, and the problem that only one influenza virus can be detected in one reaction in the conventional product is solved. The detection kit has the advantages of easiness and convenience for operating, high repeatability, quick and objective detection result and the like, and has a great application prospect in the field of in-vitro diagnosis of influenza viruses.