RPA (recombinase polymerase amplification) primer and RPA probe for detecting influenza B viruses, kit and application of RPA primer

Abstract

The invention belongs to the field of molecular biological detection, and relates to an RPA (recombinase polymerase amplification) primer and an RPA probe for detecting influenza B viruses, a kit andapplication of the RPA primer. The sequences of upstream and downstream primers are B-F and B-R, and the sequence of the probe is represented by SEQ ID. NO. 2. The RPA primer and the RPA probe for detecting the influenza B viruses can be used for rapidly detecting the influenza B viruses or simultaneously detecting influenza A and B viruses; the RPA downstream primer for the influenza B viruses can serve as a PCR amplification primer for the influenza B viruses. When the RPA primer and the RPA probe are used for detecting the influenza B viruses, the detection time is short, sensitivity and specificity are high, and the influenza A and B viruses can be simultaneously detected rapidly, accurately and specifically.

Description

technical field [0001] The invention relates to the technical field of molecular biology detection, in particular to an RPA primer, probe, kit and application for detecting influenza B virus. Background technique [0002] Influenza is mainly an infectious respiratory disease caused by influenza viruses A (FluA) and B (Flu B), with a global death toll of up to 650,000. Usually, influenza requires antiviral treatment within 48 hours of the onset of illness, and rapid diagnosis of influenza virus can significantly reduce the abuse of antibiotics and implement necessary infection control measures. Under normal circumstances, doctors will realize the diagnosis of influenza virus based on the patient's clinical symptoms. However, other viruses or bacteria can also cause flu-like illness. Therefore, diagnosis based on clinical symptoms can easily lead to misdiagnosis of the disease. Although virus isolation and culture serve as the gold standard for diagnosing influenza viruses,...

AI Technical Summary

Problems solved by technology

Polymerase chain reaction (PCR) technology is a common method for molecular amplification, but PCR technology has high requirements for hardware equipment, instruments are usually expensive, and the amplification speed is slow, so the existing recombinase polymerase amplification (RPA ) technology came into being. RPA technology has low requirements on hardware equipment. It is an isothermal amplification technology, which greatly improves the reaction speed.

The key to RPA technology lies in the design of probes and primers, but most of the probes and primers used in PCR amplification are not suitable for RPA technology

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