The invention relates to the technical field of
biomedicine, and discloses an experimental method for treating
osteomyelitis by using human
umbilical cord mesenchymal stem cells. The method aims at solving the problems that an existing
osteomyelitis experimental model is disjointed from clinical
pathological characteristics, the activity loss of mesenchymal stem cells in an infection environment is large, a combined intervention scheme lacks
pathological suitability, and an
evaluation system is single. The method comprises the following steps: firstly, constructing a clinical adaptive model by combining pathogenic bacterium injection with
immunosuppression and
ischemia induction, then carrying out anti-oxidation and targeted
colonization double pretreatment on MSC, then implementing stepped combined intervention according to the requirements of acute-stage bacterium control and chronic-stage repair, and comprehensively evaluating through
infection control, immune balance and bone repair multi-dimensional indexes, so as to obtain a bone repair model. The constructed model is closer to clinical
pathological characteristics, the MSC activity and
colonization ability are stable, the intervention scheme
collaboration is high, the
evaluation result is comprehensive and reliable, a standardized tool can be provided for
preclinical research of
osteomyelitis treatment schemes, and the conversion guidance value of the experimental result is improved.