The present invention belongs to the field of
artificial organ manufacturing technology, and in particular, relates to a special mold and a method for preparing living organs. It overcomes the difficulty of the prior art in realizing the precise construction of various shapes of artificial organs containing multi-
branch vertical channels. It includes a bottom mold, at least one inner mold and at least one outer mold; the inner wall of the bottom mold forms an annular step with a
radius increasing step by step from bottom to top, and a groove is arranged on the bottom surface of the bottom mold; the number of outer molds corresponds to the number of step
layers, and the outer mold is a sleeve-shaped structure with two ends open; the outer mold is placed on the corresponding step to form an open-top structure; the number of inner molds is the same as the number of grooves, and each inner mold corresponds to a groove, and each inner mold includes a transverse mold for simulating secondary
branch channels of blood vessels, bile ducts, nerves, and trachea, and a longitudinal mold for simulating
primary channel branches; when in use, the longitudinal mold is embedded in the corresponding groove of the bottom mold, and the
diameter of the groove is completely consistent with the
diameter of the longitudinal mold.