85 results about "Norovirus" patented technology

The invention is applicable to the technical field of virus detection, and provides a norovirus detection kit based on a photo-thermal isothermal amplification technology and a detection method, the norovirus detection kit comprises a nucleic acid amplification chamber, the nucleic acid amplification chamber is provided with reaction micropores for nucleic acid amplification; an RT-PRA system, a CRISPR-cas12a system, a blank control and a positive control are placed in the reaction micropore, the RT-PRA system comprises RT-PRA solutions R1-R5, and the CRISPR-cas12a system comprises CRISPR-Cas12a solutions C1-C3; and the photo-thermal film covers the nucleic acid amplification chamber and is used for converting sunlight into heat energy and radiating the heat energy to liquid in the reaction micropores so as to provide reaction temperature for nucleic acid amplification. According to the invention, the portable, rapid and accurate detection of norovirus infection can be realized, the detection cost can be effectively reduced, the application scene is wide, workers can be helped to deduce the virus transmission path, and key support is provided for investigation and analysis of norovirus epidemic situations.

The invention relates to the technical field of microbiological detection, and particularly discloses a primer probe combination for detecting six food-borne pathogens and application of the primer probe combination. The detection primer probe combination for the six food-borne pathogens comprises six pairs of specific primer pairs and six probes, the nucleotide sequences of the specific primer pairs are respectively shown as SEQ ID NO.1, SEQ ID NO.4, SEQ ID NO.8, SEQ ID NO.1, SEQ ID NO.13, SEQ ID NO.16, SEQ ID NO.19, SEQ ID NO.22, SEQ ID NO.26, SEQ ID NO.29, SEQ ID NO.32 and SEQ ID NO.35, and the nucleotide sequences of the probes are respectively shown as SEQ ID NO.37-SEQ ID NO.42; the six food-borne pathogens comprise listeria monocytogenes, salmonella, vibrio parahaemolyticus, norovirus, escherichia coli O157: H7 and staphylococcus aureus. According to the present invention, the pathogens of six common food-borne diseases can be detected, the detection method has characteristics of high specificity and high sensitivity, and the pathogens do not interfere with each other and do not inhibit each other during the detection so as to achieve good stability.

The invention provides a sexavalent norovirus immune composition. The sexavalent norovirus immune composition comprises virus-like particles or active fragments of GI.1 type norovirus, GII.2 type norovirus, GII.3 type norovirus, GII.4 type norovirus, GII.6 type norovirus and GII.17 type norovirus. The invention also provides a kit comprising the sexavalent norovirus immune composition. The invention also provides application of the sexavalent norovirus immune composition and the kit. The composition or the kit provided by the invention can realize effective immunization aiming at six valence types of GI.1 type, GII.2 type, GII.3 type, GII.4 type, GII.6 type and GII.17 type, and can be effective for a long time after administration.

This invention discloses a method for preparing A, B, and Lewis blood group antigens, wherein the A, B, and Lewis blood group antigens are specifically blood group A. tri Antigen, B tri Antigen, Le a Antigen and Le b Antigen. A one-pot photosensitive method was used to achieve blood group A. tri Antigen, B tri Antigen, Le a Antigen and Le b The method for obtaining large quantities of antigens, as described in this invention, features mild reaction conditions, shortened purification steps, simplicity, high efficiency, environmental friendliness, low cost, and high yield and purity of the target substance. This invention provides a practical and rapid chemical synthesis route for blood type A antigens. tri Antigen, B tri Antigen, Le a Antigen and Le b Antigens provide tools for the development of norovirus vaccines and related antibodies, and therefore have great application prospects.

The invention discloses a large-scale closed public place aerosol virus enrichment monitoring system based on an STM32 main control platform, which combines an air sampler and a microfluidic analysis technology, adopts a temperature and humidity real-time monitoring module, optimizes an air sampling and virus enrichment process, and realizes that an integrated microfluidic analysis unit completes rapid determination of virus concentration. According to the system, virus concentration detection is carried out on an enriched sample by utilizing a double-antibody sandwich enzyme-linked immunoassay technology, and the virus concentration is determined through absorbance measurement. Experiments show that the detection error of the system is lower than 5%, and virus monitoring which is quick in response, convenient to operate and low in cost can be achieved. The system is suitable for monitoring viruses such as influenza, new coronavirus and norovirus in large closed public places, and provides efficient technical support for public health safety.

Disclosed herein are vaccine compositions, in particular, polyvalent icosahedral compositions for antigen presentation. The disclosed compositions may contain an S particle made up of recombinant fusion proteins. The recombinant fusion proteins may include a norovirus (NoV) S domain protein, a linker protein domain operatively connected to the norovirus S domain protein, and an antigen protein domain operatively connected to said linker.

The invention provides a method for predicting the norovirus removal rate of a sewage treatment plant based on machine learning. The method comprises the following steps: acquiring the treatment process and scale data of the current sewage treatment plant; performing result prediction through a pre-trained prediction model based on the processing process and scale data; determining the norovirus removal rate of the current sewage treatment plant and the norovirus concentration in the effluent based on the predicted result; wherein the pre-trained prediction model is obtained by training, verifying and testing a plurality of preset machine learning models through a pre-acquired data set, and the pre-acquired data set comprises treatment processes and scale data of a plurality of known sewage treatment plants, as well as influent norovirus concentration data and effluent norovirus concentration data. The problem that an existing sewage treatment prediction model needs to firstly perform long-time sequence sampling and detection on multiple water quality indexes in inflow water and outflow water of a current sewage treatment plant, and then the model can be established for prediction is solved.

The invention provides a norovirus genome splicing method and device, a medium and a product. The method comprises the following steps: acquiring a norovirus gene database, wherein the norovirus gene database comprises a non-structural protein coding region sub-library and a capsid protein coding region sub-library; obtaining original sequencing data; performing quality control on the original sequencing data to obtain screened sequencing data; comparing and assembling the screened sequencing data with the non-structural protein coding region sub-library, and determining at least one first recombinant coding region; and comparing and assembling the screened sequencing data with the capsid protein coding region sub-library, and determining at least one second recombinant coding region, and splicing the at least one first recombinant coding region and the at least one second recombinant coding region to obtain a recombinant genome sequence. Through the technical scheme disclosed by the invention, efficient and accurate splicing of the recombinant norovirus genome sequence is realized, and the assembly accuracy and efficiency of the recombinant norovirus genome sequence are improved.

The purpose of the present invention is to provide: a human norovirus inactivation agent having an excellent human norovirus inactivation effect; and a sanitary material. The present invention relates to a human norovirus inactivation agent characterized by having a pH of 1 to 6 and comprising (a) ethanol and (b) at least one acid selected from the group consisting of gluconic acid, fumaric acid, lactic acid, malic acid, succinic acid, citric acid, and phosphoric acid.

The invention discloses an FUT2 high-expression cell strain as well as application and a method of the FUT2 high-expression cell strain in vaccine quality control, and belongs to the technical field of vaccines. The technical problem to be solved is that a simple and easy-to-operate cell model is lacked in the prior art, and the cell model is used for evaluating the effectiveness of NoV vaccines on the cellular level. According to the key points of the technical scheme, the FUT2 high-expression cell strain is provided, the cell strain is an HEK 293T cell strain (HEK 293T-FUT2-EGFP) with EGFP fluorescence for overexpressing human alpha 1, 2-fucosyltransferase 2 (FUT2), and the cell strain stably expresses H, Lewis b and Lewis y type HBGA antigens; meanwhile, an alternative neutralization experiment of the cell level of the norovirus vaccine based on the cell strain is developed, a basis is provided for interaction research of the norovirus and host cells, and the cell strain has high practical application value.

The present invention is directed to an artificial nucleic acid and to polypeptides suitable for use in treatment or prophylaxis of an infection with Norovirus or a disorder related to such an infection. In particular, the present invention concerns a Norovirus vaccine. The present invention is directed to an artificial nucleic acid, polypeptides, compositions and vaccines comprising the artificial nucleic acid or the polypeptides. The invention further concerns a method of treating or preventing a disorder or a disease, first and second medical uses of the artificial nucleic acid, polypeptides, compositions and vaccines. Further, the invention is directed to a kit, particularly to a kit of parts, comprising the artificial nucleic acid, polypeptides, compositions and vaccines.

Disclosed herein are synthetic VP1 proteins that contain engineered disulfide bonds. In some embodiments, the synthetic VP1 proteins with engineered disulfide bonds self-assemble into non-infectious virus-like particles (VLPs) that are more stable that non-infectious VLPs that resulted from self-assembly of VP1 proteins that lack engineered disulfide bonds.

A peptide that specifically binds to norovirus, which is useful for detection and infection control of norovirus is provided. A norovirus-binding peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 327.

The invention relates to the technical field of molecular biology, in particular to a primer group, a kit and a method for detecting GII type norovirus. The invention discloses a primer group for detecting a GII type norovirus. The primer group can be used for LAMP (Loop-Mediated Isothermal Amplification) to quickly amplify the sequence of the GII type norovirus. The invention further discloses a method for rapidly detecting the GII type norovirus, according to the method, a certain amount of dUTP and UDG are added into an LAMP amplification system, the probability of false positive can be reduced, meanwhile, gene amplification is conducted through LAMP, the method has the advantages of being rapid and convenient to use, an amplified product is tested through LFD, and a result can be visually displayed on LFD. The method has the advantages of rapidness, high efficiency and certain anti-pollution capability.

A pre-saturated wipe product is disclosed that is well suited for destroying non-enveloped viruses, including norovirus. The wipe composition includes a first antimicrobial agent blended with a second agent, such as a preservative. The wipe product can destroy and kill non-enveloped viruses completely in a relatively short contact time, even at very low concentrations in the wipe composition.

The invention relates to a multiplex fluorescent genotyping detection primer probe group and a kit aiming at a norovirus epidemic strain, and belongs to the technical field of biology. The primer probe group comprises a primer pair and a probe which are used for detecting the GII.17 type, the GII.3 type and the GII.4 type of the noroviruses; wherein the nucleotide sequences of the primer pair and the probe for detecting the GII.17 type are as shown in SEQ ID NO: 1-3; the nucleotide sequences of the primer pair and the probe for detecting the GII.3 type are as shown in SEQ ID NO: 4-6; the nucleotide sequences of the primer pair and the probe for detecting the GII.4 type are as shown in SEQ ID NO: 7-9. The method has the following technical effects: 1, the detection time is short, the operation is simple and convenient, and the total detection time is about 1.5 hours; 2, the sensitivity is high; 3, the detection limit is low, and the method can be applied to environmental specimen detection.

The application discloses a CRISPR-Cas12h1 system-based norovirus visual on-site detection kit and belongs to the field of virus detection. A label-free visual nucleic acid detection kit based on CRISPR-Cas12h1 is developed, and large experimental instruments and professional operators are not needed. The detection result can be read within 30 minutes under room temperature conditions, and the detection result is visible to the naked eye and can be directly read. A nucleic acid detection kit for norovirus is developed by using a CRISPR-Cas12h1 nucleic acid detection platform. The detection sensitivity and specificity of the nucleic acid detection kit are not lower than those of existing detection kits. Meanwhile, the nucleic acid detection kit has the advantages of not needing large instruments and professional equipment, short detection time, and visible results to the naked eye, which are not possessed by current detection methods.

The present invention discloses an anti-norovirus VP1 protein antibody and its applications. The present invention discloses an anti-VP1 protein antibody, its fragment or fusion protein, which comprises a heavy chain variable region and a light chain variable region. The present invention also provides an isolated polynucleotide, a vector, a host cell and a pharmaceutical composition comprising the aforementioned anti-VP1 protein antibody, its fragment or fusion protein. The present invention also discloses a method for preparing the aforementioned anti-VP1 protein antibody, its fragment or fusion protein, a method for detecting the presence or amount of VP1 protein or norovirus in a sample. The present invention also provides the use in a corresponding medicament for treating, preventing or alleviating a disease or disorder responsive to the inhibition of VP1 protein, and the use in the production of a product for detecting VP1 protein or norovirus.

The present invention discloses a soluble single-chain antibody that broadly recognizes human norovirus and a preparation method thereof, relating to the field of biotechnology. The amino acid sequence of the soluble single-chain antibody is shown in SEQ ID No. 3; the preparation method comprises: obtaining the nucleotide sequence of the soluble single-chain antibody J5D that broadly recognizes hNoV; constructing a recombinant prokaryotic expression plasmid pMAL-c5X-J5D; and prokaryotic expression and purification of J5D. The amino acid sequence of the recombinant prokaryotic expression plasmid pMAL-c5X-J5D is shown in SEQ ID No. 4. The soluble single-chain antibody J5D is used in a reagent that broadly recognizes human norovirus. The present invention provides a simple and easy method for preparing a scFv that broadly recognizes different genotypes of hNoV. An scFv that broadly recognizes different genotypes of hNoV was successfully prepared, with strong specificity, high stability, and simple operation.

To provide a method for accurately and quantitatively detecting human norovirus having activity.SOLUTION: A method for detecting a human norovirus according to the present invention is a method for detecting a human norovirus having an activity present in a test object, the method including the steps of preparing a magnetic bead-bound body in which an anti-human norovirus IgY antibody is bound to a surface of a magnetic bead, mixing the test object containing the human norovirus with the magnetic bead-bound body, collecting the magnetic bead-bound body, separating an RNA virus genome of the human norovirus bound to the magnetic bead-bound body, synthesizing cDNA from the separated RNA virus genome and amplifying the cDNA, and determining that the human norovirus having the activity is present when an amount of an amplification product obtained in the amplifying step is higher than a reference value.SELECTED DRAWING: Figure 1

The application provides a kit for detecting GI-type norovirus, a detection method and application, and belongs to the technical field of immunological detection. The kit for detecting GI-type norovirus comprises an enzyme label plate coated with GI-type norovirus VP1 protein, HRP-labeled secondary antibody, positive control, negative control, sample diluent, cleaning solution, color developing solution and termination solution. The kit is specific and high in sensitivity, and can accurately detect whether GI-type norovirus is contained in a sample. The application can greatly improve the detection efficiency of front-line inspection and quarantine personnel at ports, and maximally prevent the occurrence of imported infectious diseases.

The invention discloses a rapid detection method, a composition and a kit for GII type norovirus based on RPA-CRISPR / Cas13 and application of the rapid detection method, the composition and the kit. The composition comprises a pair of RPA (recombinase polymerase amplification) primer pairs capable of realizing rapid amplification of GII type norovirus nucleic acid under isothermal conditions and a crRNA (complementary ribose nucleic acid) sequence for specifically recognizing virus target genes. By using the composition disclosed by the invention, the norovirus can be detected by two visual methods, namely a fluorescence method and a lateral chromatography test strip method, the norovirus components in environments, foods and clinical samples can be efficiently, quickly, sensitively and specifically detected, the sensitivity reaches 1.8 copies / 3 microliters, and the single detection time is 40 minutes.

To provide a human norovirus inactivating agent and a sanitary material excellent in human norovirus inactivating effect.SOLUTION: A human norovirus deactivator comprising: (a) ethanol; and (b) at least two acids selected from the group consisting of gluconic acid, fumaric acid, lactic acid, malic acid, succinic acid, citric acid, and phosphoric acid, wherein the human norovirus deactivator has a pH of 1 to 6.SELECTED DRAWING: None

The application provides application of rotavirus non-structural protein 4 in improving immune function of recombinant rotavirus subunit vaccine, wherein the application is to express the rotavirus non-structural protein 4 gene and rotavirus antigen gene in different surface rings on a norovirus P particle to obtain a recombinant protein, and the recombinant protein is the recombinant rotavirus subunit vaccine. The application improves the immune function of the recombinant rotavirus subunit vaccine by using the NSP4 protein, and parallel expression of the NSP4 gene and the VP8 antigen gene or the NSP4 antigen gene can improve the NSP4 antigen and the VP8 antigen specific IgG antibody titer, improve the neutralization ability of the VP8 antigen to the rotavirus, and improve the protection of the NSP4 antigen and the VP8 antigen to the diarrhea mice induced by the rotavirus SA11 strain.

The invention relates to the technical field of immunodetection, and discloses a norovirus specific epitope peptide sequence and application thereof. The specific epitope peptide sequence of the norovirus disclosed by the invention has the specific amino acid sequence of VGVI (Virus Growth Vaccinium) QDGTTTHNEPQQWVLPNYSGRTGHNVH (Virus Growth Vaccinium). The norovirus specific epitope peptide sequence provided by the invention has high conservative property and specificity, so that when the norovirus specific epitope peptide sequence is used for preparing a norovirus fluorescence immunochromatography test strip subsequently, the norovirus specific epitope peptide sequence can specifically target VP1 protein of the norovirus, and thus the norovirus can be specifically recognized; the detection and analysis of the norovirus are realized, and the problems that the traditional immunological detection technology has lower detection sensitivity and the identification intensity of the norovirus is insufficient due to poorer antibody specificity are solved.

The present invention relates to quaternary ammonium disinfecting cleaner compositions comprising a quaternary ammonium compound, an antimicrobial amine, and an anionic scale inhibitor. Beneficially, the compositions provide sanitizing efficacy without diminished performance and without scale formation when diluted with hard water. The compositions are suitable for inactivating and / or reducing infectious agents, particularly Norovirus, Adenovirus, and Polyomavirus.

The invention discloses a coupling protein as well as a preparation method and application thereof. According to the preparation method disclosed by the invention, in-vitro self-coupling of the rotavirus VP8 protein and the norovirus VP1 protein is realized by utilizing a Spatier-Spytag system, the prepared coupled protein can induce a neutralizing antibody with higher titer aiming at the rotavirus, and the immunogenicity of the norovirus VP1 protein is not influenced; the immunized animal / human body can generate a high-titer specific IgG binding antibody, a serum blocking antibody and a neutralizing antibody aiming at the two viruses. The coupling protein has a wide application prospect in vaccines for resisting infant diarrhea viruses (norovirus and rotavirus).

The invention provides a method for amplifying norovirus by using an intestinal organ gas-liquid interaction model, and relates to the technical field of biological medicines. Comprising the following steps: preparing a culture medium, and inducing stem cell differentiation according to the prepared culture medium to form intestinal organs; placing the differentiated intestinal organs in Transwell for continuous culture, promoting maturation of the intestinal organs under the condition of a culture medium, and forming an intestinal organ gas-liquid interaction model; extracting the norovirus, infecting the intestinal organ gas-liquid interaction model by using the norovirus, and then detecting the amplification multiple of the norovirus. The intestinal organ gas-liquid interaction model prepared by the invention is similar to human intestinal cells in composition, can realize infection and amplification of the norovirus in vitro, can be repeatedly obtained in large quantities, avoids clinical risks and ethical constraints, and is suitable for research of norovirus in-vitro infection mechanism and drug screening.

The application provides a method for improving the detection rate of norovirus in aquatic products, which comprises the following steps: first, using a proteinase K solution to enzymolysis the digestive gland of shellfish, then adding a TGBE solution to elute, then adding enzymolysis corn starch to block activated carbon to remove the RT-qPCR inhibitor in the detection sample, taking the supernatant to extract nucleic acid, and using a fluorescent quantitative PCR instrument to detect. The detection method of the application reduces the inhibitor in the shellfish sample which interferes with the fluorescent quantitative PCR by using enzymolysis corn starch. In combination with the proteinase K enzymolysis, the TGBE elution and the RT-qRCR, the norovirus in the shellfish sample is detected, and the detection rate of the shellfish sample is increased by 10-20 times in 4h compared with the national standard method. The virus recovery rate and the detection sensitivity are greatly improved, and the method is suitable for shellfish samples, and has the characteristics of simple operation, high detection sensitivity and short time consumption.

Methods of inhibiting the replication of noroviruses and coronaviruses in a biological sample or patient, of reducing the amount of noroviruses or coronaviruses in a biological sample or patient, and of treating norovirus and coronavirus in a patient, comprising administering to said biological sample or patient a safe and effective amount of a compound of Table A, or a pharmaceutically acceptable salt thereof.