46 results about "Norovirus" patented technology

The invention is applicable to the technical field of virus detection, and provides a norovirus detection kit based on a photo-thermal isothermal amplification technology and a detection method, the norovirus detection kit comprises a nucleic acid amplification chamber, the nucleic acid amplification chamber is provided with reaction micropores for nucleic acid amplification; an RT-PRA system, a CRISPR-cas12a system, a blank control and a positive control are placed in the reaction micropore, the RT-PRA system comprises RT-PRA solutions R1-R5, and the CRISPR-cas12a system comprises CRISPR-Cas12a solutions C1-C3; and the photo-thermal film covers the nucleic acid amplification chamber and is used for converting sunlight into heat energy and radiating the heat energy to liquid in the reaction micropores so as to provide reaction temperature for nucleic acid amplification. According to the invention, the portable, rapid and accurate detection of norovirus infection can be realized, the detection cost can be effectively reduced, the application scene is wide, workers can be helped to deduce the virus transmission path, and key support is provided for investigation and analysis of norovirus epidemic situations.

This invention discloses a method for preparing A, B, and Lewis blood group antigens, wherein the A, B, and Lewis blood group antigens are specifically blood group A. tri Antigen, B tri Antigen, Le a Antigen and Le b Antigen. A one-pot photosensitive method was used to achieve blood group A. tri Antigen, B tri Antigen, Le a Antigen and Le b The method for obtaining large quantities of antigens, as described in this invention, features mild reaction conditions, shortened purification steps, simplicity, high efficiency, environmental friendliness, low cost, and high yield and purity of the target substance. This invention provides a practical and rapid chemical synthesis route for blood type A antigens. tri Antigen, B tri Antigen, Le a Antigen and Le b Antigens provide tools for the development of norovirus vaccines and related antibodies, and therefore have great application prospects.

Disclosed herein are vaccine compositions, in particular, polyvalent icosahedral compositions for antigen presentation. The disclosed compositions may contain an S particle made up of recombinant fusion proteins. The recombinant fusion proteins may include a norovirus (NoV) S domain protein, a linker protein domain operatively connected to the norovirus S domain protein, and an antigen protein domain operatively connected to said linker.

The invention provides a norovirus genome splicing method and device, a medium and a product. The method comprises the following steps: acquiring a norovirus gene database, wherein the norovirus gene database comprises a non-structural protein coding region sub-library and a capsid protein coding region sub-library; obtaining original sequencing data; performing quality control on the original sequencing data to obtain screened sequencing data; comparing and assembling the screened sequencing data with the non-structural protein coding region sub-library, and determining at least one first recombinant coding region; and comparing and assembling the screened sequencing data with the capsid protein coding region sub-library, and determining at least one second recombinant coding region, and splicing the at least one first recombinant coding region and the at least one second recombinant coding region to obtain a recombinant genome sequence. Through the technical scheme disclosed by the invention, efficient and accurate splicing of the recombinant norovirus genome sequence is realized, and the assembly accuracy and efficiency of the recombinant norovirus genome sequence are improved.

The purpose of the present invention is to provide: a human norovirus inactivation agent having an excellent human norovirus inactivation effect; and a sanitary material. The present invention relates to a human norovirus inactivation agent characterized by having a pH of 1 to 6 and comprising (a) ethanol and (b) at least one acid selected from the group consisting of gluconic acid, fumaric acid, lactic acid, malic acid, succinic acid, citric acid, and phosphoric acid.

The invention discloses an FUT2 high-expression cell strain as well as application and a method of the FUT2 high-expression cell strain in vaccine quality control, and belongs to the technical field of vaccines. The technical problem to be solved is that a simple and easy-to-operate cell model is lacked in the prior art, and the cell model is used for evaluating the effectiveness of NoV vaccines on the cellular level. According to the key points of the technical scheme, the FUT2 high-expression cell strain is provided, the cell strain is an HEK 293T cell strain (HEK 293T-FUT2-EGFP) with EGFP fluorescence for overexpressing human alpha 1, 2-fucosyltransferase 2 (FUT2), and the cell strain stably expresses H, Lewis b and Lewis y type HBGA antigens; meanwhile, an alternative neutralization experiment of the cell level of the norovirus vaccine based on the cell strain is developed, a basis is provided for interaction research of the norovirus and host cells, and the cell strain has high practical application value.

Disclosed herein are synthetic VP1 proteins that contain engineered disulfide bonds. In some embodiments, the synthetic VP1 proteins with engineered disulfide bonds self-assemble into non-infectious virus-like particles (VLPs) that are more stable that non-infectious VLPs that resulted from self-assembly of VP1 proteins that lack engineered disulfide bonds.

A pre-saturated wipe product is disclosed that is well suited for destroying non-enveloped viruses, including norovirus. The wipe composition includes a first antimicrobial agent blended with a second agent, such as a preservative. The wipe product can destroy and kill non-enveloped viruses completely in a relatively short contact time, even at very low concentrations in the wipe composition.

The invention relates to a multiplex fluorescent genotyping detection primer probe group and a kit aiming at a norovirus epidemic strain, and belongs to the technical field of biology. The primer probe group comprises a primer pair and a probe which are used for detecting the GII.17 type, the GII.3 type and the GII.4 type of the noroviruses; wherein the nucleotide sequences of the primer pair and the probe for detecting the GII.17 type are as shown in SEQ ID NO: 1-3; the nucleotide sequences of the primer pair and the probe for detecting the GII.3 type are as shown in SEQ ID NO: 4-6; the nucleotide sequences of the primer pair and the probe for detecting the GII.4 type are as shown in SEQ ID NO: 7-9. The method has the following technical effects: 1, the detection time is short, the operation is simple and convenient, and the total detection time is about 1.5 hours; 2, the sensitivity is high; 3, the detection limit is low, and the method can be applied to environmental specimen detection.

The application discloses a CRISPR-Cas12h1 system-based norovirus visual on-site detection kit and belongs to the field of virus detection. A label-free visual nucleic acid detection kit based on CRISPR-Cas12h1 is developed, and large experimental instruments and professional operators are not needed. The detection result can be read within 30 minutes under room temperature conditions, and the detection result is visible to the naked eye and can be directly read. A nucleic acid detection kit for norovirus is developed by using a CRISPR-Cas12h1 nucleic acid detection platform. The detection sensitivity and specificity of the nucleic acid detection kit are not lower than those of existing detection kits. Meanwhile, the nucleic acid detection kit has the advantages of not needing large instruments and professional equipment, short detection time, and visible results to the naked eye, which are not possessed by current detection methods.

To provide a method for accurately and quantitatively detecting human norovirus having activity.SOLUTION: A method for detecting a human norovirus according to the present invention is a method for detecting a human norovirus having an activity present in a test object, the method including the steps of preparing a magnetic bead-bound body in which an anti-human norovirus IgY antibody is bound to a surface of a magnetic bead, mixing the test object containing the human norovirus with the magnetic bead-bound body, collecting the magnetic bead-bound body, separating an RNA virus genome of the human norovirus bound to the magnetic bead-bound body, synthesizing cDNA from the separated RNA virus genome and amplifying the cDNA, and determining that the human norovirus having the activity is present when an amount of an amplification product obtained in the amplifying step is higher than a reference value.SELECTED DRAWING: Figure 1

The application provides a kit for detecting GI-type norovirus, a detection method and application, and belongs to the technical field of immunological detection. The kit for detecting GI-type norovirus comprises an enzyme label plate coated with GI-type norovirus VP1 protein, HRP-labeled secondary antibody, positive control, negative control, sample diluent, cleaning solution, color developing solution and termination solution. The kit is specific and high in sensitivity, and can accurately detect whether GI-type norovirus is contained in a sample. The application can greatly improve the detection efficiency of front-line inspection and quarantine personnel at ports, and maximally prevent the occurrence of imported infectious diseases.

To provide a human norovirus inactivating agent and a sanitary material excellent in human norovirus inactivating effect.SOLUTION: A human norovirus deactivator comprising: (a) ethanol; and (b) at least two acids selected from the group consisting of gluconic acid, fumaric acid, lactic acid, malic acid, succinic acid, citric acid, and phosphoric acid, wherein the human norovirus deactivator has a pH of 1 to 6.SELECTED DRAWING: None

The present invention relates to quaternary ammonium disinfecting cleaner compositions comprising a quaternary ammonium compound, an antimicrobial amine, and an anionic scale inhibitor. Beneficially, the compositions provide sanitizing efficacy without diminished performance and without scale formation when diluted with hard water. The compositions are suitable for inactivating and / or reducing infectious agents, particularly Norovirus, Adenovirus, and Polyomavirus.

The invention discloses a coupling protein as well as a preparation method and application thereof. According to the preparation method disclosed by the invention, in-vitro self-coupling of the rotavirus VP8 protein and the norovirus VP1 protein is realized by utilizing a Spatier-Spytag system, the prepared coupled protein can induce a neutralizing antibody with higher titer aiming at the rotavirus, and the immunogenicity of the norovirus VP1 protein is not influenced; the immunized animal / human body can generate a high-titer specific IgG binding antibody, a serum blocking antibody and a neutralizing antibody aiming at the two viruses. The coupling protein has a wide application prospect in vaccines for resisting infant diarrhea viruses (norovirus and rotavirus).

The invention provides a method for amplifying norovirus by using an intestinal organ gas-liquid interaction model, and relates to the technical field of biological medicines. Comprising the following steps: preparing a culture medium, and inducing stem cell differentiation according to the prepared culture medium to form intestinal organs; placing the differentiated intestinal organs in Transwell for continuous culture, promoting maturation of the intestinal organs under the condition of a culture medium, and forming an intestinal organ gas-liquid interaction model; extracting the norovirus, infecting the intestinal organ gas-liquid interaction model by using the norovirus, and then detecting the amplification multiple of the norovirus. The intestinal organ gas-liquid interaction model prepared by the invention is similar to human intestinal cells in composition, can realize infection and amplification of the norovirus in vitro, can be repeatedly obtained in large quantities, avoids clinical risks and ethical constraints, and is suitable for research of norovirus in-vitro infection mechanism and drug screening.

The application provides a method for improving the detection rate of norovirus in aquatic products, which comprises the following steps: first, using a proteinase K solution to enzymolysis the digestive gland of shellfish, then adding a TGBE solution to elute, then adding enzymolysis corn starch to block activated carbon to remove the RT-qPCR inhibitor in the detection sample, taking the supernatant to extract nucleic acid, and using a fluorescent quantitative PCR instrument to detect. The detection method of the application reduces the inhibitor in the shellfish sample which interferes with the fluorescent quantitative PCR by using enzymolysis corn starch. In combination with the proteinase K enzymolysis, the TGBE elution and the RT-qRCR, the norovirus in the shellfish sample is detected, and the detection rate of the shellfish sample is increased by 10-20 times in 4h compared with the national standard method. The virus recovery rate and the detection sensitivity are greatly improved, and the method is suitable for shellfish samples, and has the characteristics of simple operation, high detection sensitivity and short time consumption.

Methods of inhibiting the replication of noroviruses and coronaviruses in a biological sample or patient, of reducing the amount of noroviruses or coronaviruses in a biological sample or patient, and of treating norovirus and coronavirus in a patient, comprising administering to said biological sample or patient a safe and effective amount of a compound of Table A, or a pharmaceutically acceptable salt thereof.

The invention discloses an application of an HEK-293T cell as a norovirus in-vitro culture host cell, the HEK-293T cell is used as a host bacterium for norovirus in-vitro culture for the first time, and meanwhile, intestinal bacteria such as enterobacter cloacae are introduced as an auxiliary factor, so that the norovirus in-vitro culture efficiency is effectively improved; and a stable and efficient human norovirus in-vitro culture system is successfully established. The defects of low norovirus culture efficiency and poor stability in the prior art are overcome, and stable replication and efficient harvesting of the norovirus are realized.

The invention relates to a construction method of a multi-epitope subunit vaccine of norovirus. The method comprises the following steps: determining a standardized construction principle and a design parameter system of the multi-epitope subunit vaccine by designing four different multi-epitope construction strategies; the method solves the ubiquitous problems of single construction mode, strong immunological enhancement dependence, insufficient antigen spectrum coverage and lack of a high-throughput screening system in the existing multi-epitope vaccine design, significantly shortens the vaccine design period, reduces the experiment cost, and improves the design accuracy. The multi-epitope subunit vaccine of the norovirus is obtained through the construction method, the amino acid sequence of the multi-epitope subunit vaccine is shown as any one of SEQ ID NO.3-5, and the strong immunogenicity and broad-spectrum protection potential of the multi-epitope subunit vaccine are verified through animal experiments.

The application discloses a cross primer isothermal amplification primer group, a system and application for detecting norovirus, and belongs to the technical field of medical detection. The application provides the cross primer isothermal amplification primer group for detecting norovirus, which is composed of an inner loop primer IP, a molecular beacon AMP, a reverse cross primer CPR, a forward cross primer CPF, a reverse primer RB and a forward primer FB. The cross primer isothermal amplification primer group for detecting norovirus is used to detect a nucleic acid sample as a template, and isothermal amplification is carried out by using the above-mentioned primers. After reaction, a result can be directly determined by using a detection analyzer. The application has good specificity, high accuracy, high sensitivity, simple and rapid operation, and is suitable for instant detection of norovirus.

This invention relates to a primer and probe set and kit for multiplex fluorescent genotyping detection of norovirus strains, belonging to the field of biotechnology. The primer and probe set of this invention includes primer pairs and probes for detecting norovirus types GII.17, GII.3, and GII.4; wherein the nucleotide sequences of the primer pairs and probes for detecting GII.17 are shown in SEQ ID NO: 1-3; the nucleotide sequences of the primer pairs and probes for detecting GII.3 are shown in SEQ ID NO: 4-6; and the nucleotide sequences of the primer pairs and probes for detecting GII.4 are shown in SEQ ID NO: 7-9. This invention has the following technical advantages: 1. Short detection time and simple operation, with a total detection time of approximately 1.5 hours. 2. High sensitivity. 3. Low detection limit, applicable to environmental sample detection.

The present invention is directed to an artificial nucleic acid and to polypeptides suitable for use in treatment or prophylaxis of an infection with Norovirus or a disorder related to such an infection. In particular, the present invention concerns a Norovirus vaccine. The present invention is directed to an artificial nucleic acid, polypeptides, compositions and vaccines comprising the artificial nucleic acid or the polypeptides. The invention further concerns a method of treating or preventing a disorder or a disease, first and second medical uses of the artificial nucleic acid, polypeptides, compositions and vaccines. Further, the invention is directed to a kit, particularly to a kit of parts, comprising the artificial nucleic acid, polypeptides, compositions and vaccines.

The application belongs to the technical field of biotechnology, and particularly relates to an anti-GII.6 norovirus antibody or antigen-binding fragment thereof, and a preparation method and application thereof. The antibody or antigen-binding fragment thereof can specifically recognize and bind to GII.6 norovirus or VP1 protein thereof, and has good affinity therewith, and has no cross reaction with GI.1, GII.2, GII.3, GII.4 and GII.17 norovirus or VP1 protein thereof. Meanwhile, the antibody or antigen-binding fragment thereof has good blocking activity on GII.6 norovirus VLP. The antibody or antigen-binding fragment thereof can be used for preparing products for diagnosing, preventing and / or treating GII.6 norovirus infection or diseases caused by the GII.6 norovirus, or detecting the presence or level of GII.6 norovirus or VP1 protein thereof in a sample.

The invention discloses a system, a device and a method for detecting norovirus double genotypes by a one-step method, provides a system based on CRISPR / Cas12a and CRISPR / Cas13a combined with RAA amplification, can detect GII and GI genotypes of norovirus by a one-step method, and designs a device for detecting GII and GI genotypes of norovirus by a one-step method. The GII and GI genotypes of the norovirus can be rapidly and visually detected at the same time, the method has the advantages of being high in specificity and detection efficiency, and the problem that in the prior art, the detection efficiency is low is solved.

The invention discloses a multi-target fluorescence immunoassay method based on TMSD-DNAzyme, which is used for simultaneously detecting staphylococcus aureus enterotoxin B (SEB) and GII type norovirus (NoV), and comprises the following steps: S1, coating a 96-hole black microplate with an SEB capture antibody, and sealing; s2, a diluted immune probe and a sample to be detected are added, a sandwich structure is formed through an immune reaction, and the immune probe is formed by coupling an SEB detection antibody and a Trigger chain on the surface of a gold nanoparticle; according to the invention, by designing two sets of independent signal amplification channels which can run in parallel, the detection of SEB protein and NoV nucleic acid is completed in one reaction hole at the same time, compared with a traditional mode needing two independent ELISA detection, the method has the advantages that the detection process is simplified by nearly one time, the real'one-time detection and double results' is realized, and the detection efficiency is greatly improved. The detection flux is greatly improved, and about 50% of sample, reagent and time cost is saved.

The purpose of the (Fort's Gloves, Dry and Hold Pail) is to prevent moisture that causes the spread of bacteria from restrooms that is the cause of influenza, streptococcus, E coli, Hepatitis, MRSA, Salmonella, Shigella, and Norovirus. The pail is a new creation except for the authentic manuscript and cursive text. There is a distinct difference from the usual and traditional utility pails each community uses, because of the holder for the moisture elimination products that prevents contaminated water from spilling to the floor. Fecal and Trash water could potentially cause mildew growth on the gloves and the pail. The Dry & Hold container overall is a new Invention and a cleaner choice for the environment because the design prevents disease, foul odors.

The invention belongs to the field of biological medicine and molecular biology, and particularly relates to novel orthogonal replication RNA (Ribonucleic Acid) as well as a preparation method and application thereof. The sequence of the orthogonally replicated RNA comprises: (a) a 5'untranslated region of the human norovirus; (b) an open reading frame 1 of human norovirus encoding a replicase; (c) an antisense coding sequence of the target gene; (d) replicating a termination signal and a 3'untranslated region; after the orthogonally replicated RNA is replicated in cells, sense mRNA which is covalently connected with Vpg protein at the 5'end and can translate the target gene can be generated. According to the application, the huNoV replicon is successfully engineered into a therapeutic OrepRNA platform for the first time, a brand new RNA drug carrier category is created, low immunogenicity and high fidelity can be realized, and the disease treatment bottleneck of cap-dependent translation limitation can be broken through. According to the platform, various diseases with different pathological mechanisms can be solved by matching different treatment genes, the ductility is high, and a breakthrough treatment scheme is provided for refractory diseases such as tumor cachexia and GVHD pulmonary fibrosis.

The invention belongs to the field of biomedical treatment, and particularly relates to a rotavirus, enteric adenovirus and norovirus detection kit. A convection amplification primer group in the kit comprises an upstream primer and a downstream primer, each of the upstream primer and the downstream primer comprises a complementary sequence complementary with target nucleic acid and a tailed sequence, and the tailed sequence is located at the 5'end of the complementary sequence and is not complementary with the target nucleic acid; the complementary sequence has a folded sequence, and the folded sequence is complementary with the tailing sequence, so that the primer sequence has a curved hairpin structure; the enterovirus is a norovirus, an enteroadenovirus and a rotavirus. Meanwhile, a primer probe is designed, and a kit for simultaneously detecting multiple enteroviruses is prepared. The kit can avoid non-specific amplification and improve the detection sensitivity.

The invention discloses a cold-chain food outer package sterilization system and method for norovirus, and relates to the technical field of virus sterilization, and the system comprises a conveying unit, a low-energy electron beam irradiation unit, a dose monitoring and control unit, an overturning unit and a safety protection unit. The conveying unit conveys packages, the low-energy electron beam irradiation unit generates 120-300 keV electron beam irradiation, the dose monitoring and control unit controls the absorbed dose to be 5-10 kGy, the overturning unit cooperates with other units through a distributed control system to achieve automatic overturning, and the safety protection unit guarantees safety. According to the method, disinfection is completed through conveying positioning, primary irradiation, overturning and secondary irradiation. The system and the method can efficiently and thoroughly kill norovirus, are safe and reliable, are high in automation degree, adapt to a cold chain environment and make up for the defects of a traditional killing mode.