Primer and method for detecting GI type and GII type norovirus by utilizing primer

A virus and primer sequence technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganism measurement/testing, etc., can solve the problem of low detection sensitivity and achieve simplified operation steps, shortened detection time, and high specificity Effect
CN102304514AInactive Publication Date: 2012-01-04湖州市疾病预防控制中心
6 Cites 13 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
湖州市疾病预防控制中心
Publication Date
2012-01-04
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a primer and a method for detecting GI type and GII type norovirus by utilizing the primer, belonging to the technical field of biological detection. The sequence of the primer is as follows: Nov-F: 5-GTGAATGAWGATGGCGTCKA-3, Nov-R: 5-GTHAGWATCCAGGGGTCAAT-3. The invention focuses on the design of the sequence of the primer, the composition of an RT-PCR (reverse transcription-polymerase chain reaction) system, the selection of reaction conditions and the judgment of reaction results can be performed according to the conventional methods in the field. By adopting the primer and the method provided by the invention, a pair of primers can simultaneously detect and differentiate the GI type and the GII type norovirus in a same reaction tube, thereby not only simplifying the operation steps, but also shortening the detection time.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a primer and a method for detecting GⅠ and GⅡ norovirus using the primer, which belongs to the technical field of biological detection. Background technique

[0002] Noroviruses (NVs), belonging to the Norovirus genus of the Caliciviridae family, are important pathogens that cause outbreaks and sporadic nonbacterial gastroenteritis worldwide. At present, norovirus can be divided into five genetic groups (GenogroupⅠ~Ⅴ, GⅠ~Ⅴ) according to the nucleotide sequence differences of the viral gene RNA polymerase (RNA dependent RNA polymerase, RdRp) and the main capsid protein VP1. Humans are mainly infected with GⅠ and GⅡ noroviruses, and each genetic group can be further divided into several genotypes. Because the virus has strong resistance, low pathogenic dose, and people of all ages are generally susceptible to it, it can often cause large-scale outbreaks in some public places such as schools, kindergartens, hospitals, etc. throug...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More