156 results about "GenBank" patented technology

The invention discloses a computer network security situation awareness system and method, and relates to the technical field of computer network security, and the method comprises the steps: generating a dynamic gene pool through an antigen-antibody matching algorithm based on an initial quantum trust vector, and building a mapping relation table of an antigen feature vector and an antibody defense strategy; triggering a quantum alarm according to an entanglement entropy mutation event in the real-time quantum state flow data, matching a biological antibody strategy corresponding to a current network attack feature based on a mapping relation table, and fusing the quantum alarm and the biological alarm to generate an attack path topological graph; the network security situation is perceived by analyzing the quantum state change of the abnormal node ID and combining an attack path topological graph and a dynamic gene pool. Quantum entanglement pairs are distributed to network nodes through the quantum key distribution center, and initial quantum trust vectors are generated, so that the unknown attack resisting capability of the network is remarkably improved, and efficient and accurate network security situation awareness is realized.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus velezensis and application thereof. The bacillus velezensis strain is separated and screened from rhizosphere soil of wheat in a main wheat area in Hebei Province, the growth characteristics and physiological and biochemical characteristics of the bacterial colony are identified, then 16S rDNA sequencing is carried out, Blast homology comparison is carried out in a GenBank database, the bacterial strain is identified to be bacillus velezensis through the analysis, and the bacillus velezensis strain is identified to be bacillus velezensis. The fungicide containing the bacillus velezensis has a good prevention and treatment effect on wheat stem rot through seed coating.

The invention provides a specific monoclonal antibody for detecting rotavirus and application of the specific monoclonal antibody, aiming at an epidemic strain of porcine rotavirus, reference to a gene sequence of VP6 protein published by GenBank, antigen epitopes are further optimized, and purified recombinant VP6 protein with antigenicity is obtained for animal immunization. The monoclonal antibody capable of specifically recognizing the porcine rotavirus is successfully screened out. The monoclonal antibody obtained through screening can effectively recognize epidemic strains, has broad-spectrum recognition, is superior to commercially available antibodies, and has high antigen recognition target performance and low non-specificity. And the sequence background is clear, large-scale amplification and production can be carried out, and the method has good commercial value.

The invention discloses a self-assembly enzyme for directional synthesis of uniform low molecular weight dextran, the self-assembly enzyme is named as YG-SpyTag-SpyCatcher-padex, the YG is a dextran sucrase gene (Genbank No.DQ345760), the padex is a dextran enzyme gene (Genbank No.KF999646.1), the SpyTag and the SpyCatcher are two specific oligopeptides, and aspartic acid in the SpyTag and lysine in the SpyCatcher spontaneously form an isopeptide bond. The invention belongs to the technical field of biology, and relates to an enzyme engineering technology. According to the invention, dextran sucrase derived from an escherichia coli genetically engineered bacterium BL21 (DE3)-YG-SpyTag and dextran enzyme derived from a pichia pastoris genetically engineered bacterium X33-SpyCatcher-padex are combined through a SpyTag-SpyCatcher protein covalent coupling system, and the obtained self-assembled enzyme can be used for catalyzing the substrate sucrose to be directly converted into dextran with the molecular weight of 3000 to 4000 Da.

The invention provides a genetically engineered bacterium and a construction method of the genetically engineered bacterium, and the genetically engineered bacterium is characterized in that a green fluorescent protein sfGFP is used for mediating protein glutaminase, PG (protein glutaminase), PGF (protein glutaminase), PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF and PGF; eC 3.5. 1.44) is efficiently secreted and expressed in engineering bacteria, and belongs to the technical field of biological engineering. The method comprises the following steps: an obtained sfGFP gene is derived from an NCBI database (GenBank numbering: CP035486.1), an obtained PG zymogen gene PP (Propeptide-Protein glutaminase) is derived from a Chryseobacterium prion prgA gene (GenBank numbering: AB046594.1) in the NCBI database, a recombinant plasmid pHT01 / sfGFP-PP is introduced into bacillus subtilis WB800N to obtain an engineering bacterium, extracellular secretion expression of PP is realized, the sfGFP-PP is activated by trypsin to obtain PG, and the PG is subjected to enzyme activation to obtain the recombinant bacillus subtilis. The expression quantity and the secretion efficiency of the PG in the bacillus subtilis are greatly improved. The recombinant PG produced by the method can generate 23.5 U / mL enzymatic activity under activation of trypsin, can be used for improving protein functional characteristics, and has relatively high industrial production and application values.

The invention discloses a structural variation marker based on an ARL13B gene and applied to Wenchang chicken breeding and a detection method of the structural variation marker, and relates to agricultural bioengineering, the sequence of the structural variation molecular marker is SEQ ID No: 01, and the structural variation molecular marker is located on a Wenchang chicken gene reference genome ASM4043665v1, Gallus gallus chick; the molecular marker is characterized in that the position of Genbank: GCA040436655.1 is CM080655.1: 108497195-108497244, and the molecular marker with the structural variation is a deletion site; at the same week age, the weight of an individual with the deletion structure variation marker sequence is obviously higher than that of a non-deletion individual, and the molecular marker obtained by the invention can be applied to Wenchang chicken breeding.

The invention discloses an application of a rice OsRNU7 gene. The registration number of the nucleotide sequence of the OsRNU7 gene in Genbank is LOC4337677, and the registration number of the nucleotide sequence of the OsRNU7 gene is LOC4337677. RNA-seq is utilized to discover that the OsRNU7 shows response to the external nitrogen supply level, and fluorescent quantitative PCR (Polymerase Chain Reaction) proves that the OsRNU7 is induced by high nitrogen. The OsRNU7 is knocked out, so that the nitrogen absorption rate, the plant height and the yield of the rice are reduced, and the comprehensive performance is that the nitrogen fertilizer utilization efficiency is reduced. The function of the OsRNU7 gene for positively regulating the utilization efficiency of the rice nitrogen fertilizer is defined, and a technical route for improving the utilization efficiency of the rice nitrogen fertilizer by increasing the expression of the OsRNU7 in rice is provided.

The invention discloses a genetically engineered virus strain of an African swine fever virus, and in a genome of the genetically engineered virus strain, a D250R gene is an inactivated gene. The microbial preservation number of a parent strain for preparing the genetic engineering virus strain is CCTCC (China Center For Type Culture Collection) NO: V 202318, and the GenBank accession number of a genomic sequence of the strain is OQ504956.1. The invention also discloses a preparation method of the genetically engineered virus strain, a vaccine composition containing the genetically engineered virus strain, a preparation method of the vaccine composition, and application of the genetically engineered virus strain in preparation of a preparation for preventing African swine fever. The genetically engineered virus strain is good in safety and immune effectiveness, and has the potential of preparing clinically applied vaccines.

The invention relates to the technical field of biological genetic engineering, in particular to construction of recombinant lactobacillus plantarum of an African swine fever virus antigen, and the method comprises the following steps: constructing a lactobacillus plantarum shuttle vector containing different ASFV antigen genes; and referring to a genome sequence of a Chinese isolate CADCHN09 of ASFV disclosed in GenBank. The method has the advantages that an inducer is not needed, antigen protein is efficiently expressed, the dendritic cell (DC) binding peptide for enhancing immune cell uptake and processing is subjected to fusion expression, the oral immunization mode is simple, mucosal immunity is stimulated, and ASFV infection is effectively prevented and controlled; probiotics, namely lactobacillus plantarum, which naturally exist in animal intestinal tracts and can be colonized and copied in digestive tracts for a long time are used as a carrier; key epitopes of proteins such as nucleocapsid protein p72, outer envelope protein CD2v, inner envelope protein p54, p22, pE199L and pE248R, which play an important role in adsorption, internalization, endocytosis and membrane fusion of ASFV, are preferably fused with dendritic cell binding peptide and then are displayed on the surface of lactobacillus plantarum.

The invention discloses a method for identifying duck palm weight and related molecular marker application thereof, and relates to the technical field of molecular marker-assisted selection, a primer pair is used for amplifying a DNA fragment containing a 63823029th base polymorphic site from the 5'terminal on a fourth chromosome of a duck reference genome IASAASPekinDuckT2T, and the primer pair is used for amplifying a DNA fragment containing the 63823029th base polymorphic site from the 5 '-terminal on the fourth chromosome of the duck reference genome IASAASPekinDuckT2T. The duck reference genome IAASAASPekinDuckT2T is a duck reference genome sequence in a GenBank database, and the duck reference genome sequence is a duck reference genome sequence in the GenBank database; the duck is a Chinese and new white feather meat duck, and the primer pair consists of a DNA (deoxyribonucleic acid) molecule as shown in SEQ ID No.2 and a DNA molecule as shown in SEQ ID No.3. According to the method for identifying the duck foot weight and the application of the related molecular marker, the genotype of the duck can be judged by detecting the genome DNA of the duck, early living screening of the duck foot weight character is achieved, phenotype determination after slaughtering is not needed, the breeding period is remarkably shortened, and the breeding cost is reduced.

The invention discloses a method for detecting a beta-lactamase drug-resistant gene. The method comprises the following steps: extracting bacterial plasmids; the method comprises the following steps of: downloading sequences of TEM-1 and ampC from GenBank, comparing nucleotide sequences by utilizing Megaalign, finding out a gene conserved region, and designing a primer by utilizing Primer expansion V5; and adding reactants of a PCR reaction system by taking the extracted whole-genome DNA of the bacteria and the extracted plasmid DNA of the bacteria as templates, and carrying out PCR amplification according to the reaction temperature required by the corresponding amplified substances. According to the invention, plasmid-mediated beta-lactam drug resistance detection and analysis are carried out on bovine-derived escherichia coli, so that drug resistance detection rates of TEM-1 type and ampC type genes of a whole genome are 76% and 95% (n is equal to 38); the drug resistance detection rates of the plasmid DNA are 98% and 89% (n = 44), and the drug resistance detection rates of the plasmid DNA are both 100%. The invention provides a scientific basis for further prevention and control of bovine-derived bacterial infectious diseases and reasonable use of antibacterial drugs, and also provides valuable data support and theoretical reference for guiding livestock farm medication and veterinary clinical scientific medication.

The invention discloses a preparation method of CsPHT1; the invention discloses modification of selenium element absorbed by tea and application of the gene 3 to feed, and relates to the field of agricultural gene engineering. CsPHT1 (CsPHT1); the gene 3 is GenBank: OP219455.1, gene mutation of D144K and T150V is carried out on the gene 3, and the amino acid sequence after mutation is shown as SEQ ID No: 01; tea tree CsPHT1; 3, the cultivation of D144K and T150V gene mutation seedlings is improved by the gene. The new variety cultivated by the invention can improve the selenium content and the oxidation resistance of the selenized tea polysaccharide without being influenced by the change of pH 5-7; the selenium-supplementing feed is obtained by mixing the high-selenium tea leaves with the feed, and the requirements of the current high-end feed market are met.

The invention discloses an application of a rice OsRNU6 gene. The login number of the nucleotide sequence of the OsRNU6 gene in Genbank is LOC4332833, and the login number of the nucleotide sequence of the OsRNU6 gene in Genbank is LOC4332833. RNA-seq is utilized to discover that the OsRNU6 shows response to the external nitrogen supply level, and fluorescent quantitative PCR (Polymerase Chain Reaction) proves that the OsRNU6 is induced by low nitrogen. The OsRNU6 is knocked out, so that the nitrogen absorption rate, plant height, tillering and yield of the rice are reduced, and the comprehensive performance is that the utilization efficiency of a nitrogen fertilizer is reduced. The function of the OsRNU6 gene for positively regulating the utilization efficiency of the rice nitrogen fertilizer is defined, and a technical route for improving the utilization efficiency of the rice nitrogen fertilizer by increasing the expression of the OsRNU6 in rice is provided.

The invention discloses an application of a rice OsARF24 gene. The login number of the nucleotide sequence of the OsARF24 gene in Genbank is LOC4352211, and the login number of the nucleotide sequence of the OsARF24 gene in Genbank is LOC4352211. The rice OsARF24 gene is a key element in an auxin signal path. Knockout of the OsARF1 can cause reduction of the expression quantity, the nitrate nitrogen absorption rate, the plant height and the yield of the nitrogen metabolism related genes of the rice, and the comprehensive performance is that the utilization efficiency of the nitrogen fertilizer is reduced. The function of the OsARF24 gene for positively regulating the utilization efficiency of the rice nitrogen fertilizer is defined, and a technical route for improving the utilization efficiency of the rice nitrogen fertilizer by increasing the expression of the OsARF24 in rice is provided.

The invention discloses screening of novel multifunctional selenite reductase SDR-SerA, and belongs to the field of environmental pollutants. A novel multifunctional enzyme capable of reducing selenite is screened from a gene bank in a selenium-rich region, and the reduction activity of the enzyme to S-1-phenylethanol is further represented. According to the invention, selenite reductase is mutated, and site-specific mutagenesis and molecular dynamics (MD) simulation reveal that the mutant M206 affects a lid opening and closing mechanism of an enzyme active site. The novel selenite reductase is reported from a short-chain dehydrogenase / reductase (SDR) superfamily, and a novel method is provided for finding the novel selenite reductase from a wide enzyme library of the novel selenite reductase. The research not only deepens the understanding of the selenium metabolism process, but also lays a foundation for developing an efficient selenium pollution bioremediation technology.

The invention discloses a lung cancer drug resistance prediction method based on user feature analysis, and relates to the technical field of biological information processing, and the method comprises the steps: calling a biological gene pool, analyzing and mining a gene interaction network based on gene variation statistics of targeted drugs, supervising and training a drug resistance detector based on a self-attention mechanism, receiving user inspection data, and predicting the drug resistance of the lung cancer; and controlling a sequencer to carry out gene sequencing detection, determining a user gene sequence, transmitting the user gene sequence to a drug resistance detector, and determining a variation trend of a targeted fragment so as to analyze drug resistance prediction data under a time sequence. The method and the device are used for solving the technical problem that the accuracy of a prediction effect is limited due to insufficient depth and comprehensiveness of drug resistance analysis in the prior art. Drug resistance analysis is carried out based on variation prediction of the target gene, and the accuracy of drug resistance prediction can be effectively improved.

The invention relates to the technical field of biological medicine, and discloses an anti-human 5T4 single-chain antibody, the antibody has the capability of specifically binding to human 5T4 protein, the human 5T4 protein corresponds to TPBG / WAIF1, and the GenBank login number is NP001018111.1; the antibody also has the capability of specifically binding and expressing human 5T4 protein tumor cells, and the human 5T4 protein tumor cells are breast cancer cells MCF-7, prostate cancer cells PC3 or gastric cancer cells MGC-803. By combining single B cell culture with a magnetic bead sorting technology, natural pairing of a heavy chain and a light chain of an antibody can be reserved, and the defect that pairing is damaged by a traditional hybridoma technology is overcome; meanwhile, false positive clones are effectively eliminated through double positive screening of detecting protein binding activity through ELISA and detecting cell binding activity through FACS, the finally obtained targeting 5T4 monoclonal antibody can be specifically combined with human 5T4 protein and tumor cells expressing the protein, and a high-quality targeting molecular basis is provided for research and development of subsequent antibody drugs and CAR-T cell treatment products.

The invention discloses an application of a TaERF9 gene in cooperation with histone modification to participate in nitrogen-mediated root development. The homologous genes of three subgenomes of the TaERF9 gene respectively correspond to the GenBank numbers of CM022223.1, CM022224.1 and CM022225.1 in NCBI (National Center of Biotechnology Information). According to the invention, wheat KN199 is directionally edited by using a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated protein 9) technology, so that a mutant plant with TaERF9 function deletion is obtained. Protein interaction experiments prove that the TaERF9 can interact with the subunit SWN of the PRC2. The results of high-nitrogen and low-nitrogen water culture experiments show that TaERF9 gene mutation significantly promotes root development under a high-nitrogen condition; under the low-nitrogen condition, the root development of the mutant is kept similar to that of a wild type, the ability of balancing the root development under different nitrogen supply conditions is shown, and the effect of the TaERF9 gene in nitrogen response mediated root development regulation is revealed. That is to say, the TaERF9 gene can promote root development after mutation, so that a gene resource is provided for nitrogen-efficient breeding of crops.

The invention discloses an application of protein kinase CPK28 (GenBank: AT5G66210) in endowing or enhancing aluminum resistance of plants, and the CPK28 is overexpressed in the plants, so that a zinc finger transcription factor STOP1 can be phosphorylated, and the protein accumulation of the STOP1 is improved, thereby enabling the plants to generate or enhance the aluminum toxicity resistance. The invention provides a theoretical basis for culturing aluminum toxicity resistant crops.

The application discloses application of a rice metal tolerance protein coding gene OsMTP7 The rice metal tolerance protein coding gene OsMTP7 has the accession number of AK241225.1 in Genbank. The gene coding protein has zinc transport activity, can increase the zinc content of rice root system and above-ground part, and significantly increases the accumulation of zinc in rice grains.

The invention discloses a chloroplast gene ITS2 bar code sequence primer composition, an identification kit and application. The primer composition contains at least one pair of primers which are derived from a nucleotide sequence with the login number of PP277665.1 and / or PP906956.1 from GenBank. The primer composition can effectively identify and / or distinguish patrinia scabiosaefolia, thlaspi arvense and a mixture of the patrinia scabiosaefolia and the thlaspi arvense And the identification method is simple and reliable, has strong objectivity, does not depend on subjective judgment, is compatible with various instruments and reaction systems, is not easily influenced by sample characters, and can be used for accurately identifying the medicinal material powder.

The invention discloses an application of an indica rice OsRNU2 gene. The registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161, and the registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161. The difference of the indica rice OsRNU2 gene promoter is the reason for the utilization efficiency and yield of indica rice and japonica rice nitrogen fertilizer, and the indica rice OsRNU2 gene promoter sequence is shown as SEQ ID NO.2. In the background of Huajing indica 74, 159 parts of materials carrying IRAT261 single fragment replacement lines are used for QTL (quantitative trait loci) analysis, and the site qRNU2 influencing the nitrate nitrogen absorption rate is positioned. The OsRNU2 haplotype expression level in indica rice is low, so that the indica rice can accumulate more auxin. The excellent allelic variation of the OsRNU2 in the indica rice is introduced into the japonica rice, so that the nitrogen fertilizer utilization efficiency and yield of the japonica rice can be improved.

The application discloses application of a rice OsARF24 gene. The nucleotide sequence of the OsARF24 gene has an accession number of LOC4352211 in Genbank. The rice OsARF24 gene is a key element in an auxin signal pathway. Knocking out the OsARF1 can cause the expression amount of nitrogen metabolism related genes of the rice, the nitrate nitrogen absorption rate, the plant height and the yield of the rice to be reduced, and the nitrogen fertilizer utilization efficiency to be reduced in a comprehensive manner. The function of the OsARF24 gene in positively regulating the nitrogen fertilizer utilization efficiency of the rice is clarified, and a technical route for increasing the nitrogen fertilizer utilization efficiency of the rice by increasing the expression of the OsARF24 in the rice is provided.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

The invention discloses a method for identifying rhizoma cibotii medicinal material base and adulterants thereof based on rbcL bar codes, and belongs to the technical field of medicinal material base molecular identification. The rbcL sequence of the rhizoma cibotii species is constructed by collecting rhizoma cibotii and adulterants thereof from different producing areas and combining the adulterants sequence downloaded from GenBank, K2P genetic distance analysis and phylogenetic tree construction are carried out based on the rbcL bar code, and it is determined that the rbcL is the optimal DNA bar code of rhizoma cibotii. The rbcL gene amplification primer is adopted to perform PCR amplification on the DNA sequence of the to-be-detected sample to obtain the corresponding sequence, the K2P genetic distance is analyzed and the phylogenetic tree is constructed according to the obtained sequence matrix, species can be obviously separated, the cibotium rhizome medicinal material and the source species of the adulterants can be clearly distinguished, and the method is simple and convenient to operate. A brand-new thought is provided for identification of the rhizoma cibotii medicinal material base and adulterants of the rhizoma cibotii medicinal material base, and the method has important significance for solving the problem of adulteration of rhizoma cibotii medicinal materials in the medicinal material

This invention relates to a logistics transportation method based on electronic fences, belonging to the field of logistics transportation. The method includes: selecting process DNA matching the logistics task to be processed from a gene bank as parent DNA; performing genetic processing on the parent DNA to obtain multiple offspring DNAs; selecting one of the offspring DNAs as the master DNA based on digital twin technology; generating a dynamic electronic fence based on the master DNA; calculating a deviation vector based on acquired current state data and future state trajectory prediction data; when the deviation vector meets preset conditions, determining the gene segment to be mutated in the master DNA based on the deviation vector and performing mutation processing to obtain multiple variant DNAs; selecting one of the variant DNAs as the optimal variant DNA based on digital twin technology and current state data; updating the master DNA and the dynamic electronic fence based on the optimal variant DNA. This method has the advantages of enabling proactive monitoring and reducing cargo damage and transportation delays.

The application provides a monoclonal antibody for specifically detecting rotavirus and an application thereof, and is directed to epidemic strains of porcine rotavirus, and refers to the gene sequence of VP6 protein published in GenBank, further optimizes an antigen epitope, obtains a purified recombinant VP6 protein with antigenicity, and performs animal immunization, and successfully screens the monoclonal antibody capable of specifically recognizing the porcine rotavirus. The monoclonal antibody screened can effectively recognize the epidemic strains, has the broad-spectrum recognition, is superior to the commercially available antibody, has high antigen recognition target specificity, is low in non-specificity, and has clear sequence background, can be scaled up and produced, and has good commercial value.

The invention discloses a screening method and application of a shark-derived anti-idiotypic nano antibody, and belongs to the technical field of biological pharmacy and vaccines. The screening method comprises the following steps: immunizing adult shark by using a target pathogen specific antigen, collecting peripheral blood, separating lymphocytes, extracting RNA (Ribonucleic Acid), carrying out reverse transcription to synthesize cDNA (Complementary Deoxyribonucleic Acid), amplifying a nano antibody variable region fragment of a shark IgNAR (Immunoglobulin Nucleic Acid Receptor) gene, and constructing a phage display gene bank of which the storage capacity is greater than or equal to 1 * 10 < 8 > CFU and the recombination rate is greater than or equal to 85%; a variable region of a target pathogen specific antibody is taken as a target, specific bacteriophage is enriched through 3-4 rounds of gradient washing and elutriation (Tween-20 concentration and washing time are gradually improved), and the shark source anti-idiotypic nano antibody with high affinity and high purity (greater than or equal to 95%) is obtained by combining ELISA screening positive clone, gene sequencing de-weighting and SPR detection (combining dissociation constant KDlt, 1 * 10 <-7 > mol / L).

The invention relates to the technical field of sea cucumber collagen, in particular to protein with a repairing effect and a preparation method and application thereof. The amino acid sequence of the protein is from Collagen [Apostichopus japonicus] GenBank: APA22677.1 in an NCBI (National Center for Biotechnology Information) database, the strain Pichia sp. With high protein expression quantity is finally found through codon optimization, further expression and screening by virtue of a shake flask and a 10L fermentation tank, the strain Pichia sp. Is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 18, 2025, and the preservation number is CGMCC No.34894. The invention further discloses a preparation method of the Pichia sp. The obtained pure protein has an excellent repairing effect and a certain anti-oxidation effect, and has a great commercial value.