82 results about "GenBank" patented technology

The invention discloses a method for identifying duck palm weight and related molecular marker application thereof, and relates to the technical field of molecular marker-assisted selection, a primer pair is used for amplifying a DNA fragment containing a 63823029th base polymorphic site from the 5'terminal on a fourth chromosome of a duck reference genome IASAASPekinDuckT2T, and the primer pair is used for amplifying a DNA fragment containing the 63823029th base polymorphic site from the 5 '-terminal on the fourth chromosome of the duck reference genome IASAASPekinDuckT2T. The duck reference genome IAASAASPekinDuckT2T is a duck reference genome sequence in a GenBank database, and the duck reference genome sequence is a duck reference genome sequence in the GenBank database; the duck is a Chinese and new white feather meat duck, and the primer pair consists of a DNA (deoxyribonucleic acid) molecule as shown in SEQ ID No.2 and a DNA molecule as shown in SEQ ID No.3. According to the method for identifying the duck foot weight and the application of the related molecular marker, the genotype of the duck can be judged by detecting the genome DNA of the duck, early living screening of the duck foot weight character is achieved, phenotype determination after slaughtering is not needed, the breeding period is remarkably shortened, and the breeding cost is reduced.

The invention discloses a preparation method of CsPHT1; the invention discloses modification of selenium element absorbed by tea and application of the gene 3 to feed, and relates to the field of agricultural gene engineering. CsPHT1 (CsPHT1); the gene 3 is GenBank: OP219455.1, gene mutation of D144K and T150V is carried out on the gene 3, and the amino acid sequence after mutation is shown as SEQ ID No: 01; tea tree CsPHT1; 3, the cultivation of D144K and T150V gene mutation seedlings is improved by the gene. The new variety cultivated by the invention can improve the selenium content and the oxidation resistance of the selenized tea polysaccharide without being influenced by the change of pH 5-7; the selenium-supplementing feed is obtained by mixing the high-selenium tea leaves with the feed, and the requirements of the current high-end feed market are met.

The invention relates to the technical field of biological medicine, and discloses an anti-human 5T4 single-chain antibody, the antibody has the capability of specifically binding to human 5T4 protein, the human 5T4 protein corresponds to TPBG / WAIF1, and the GenBank login number is NP001018111.1; the antibody also has the capability of specifically binding and expressing human 5T4 protein tumor cells, and the human 5T4 protein tumor cells are breast cancer cells MCF-7, prostate cancer cells PC3 or gastric cancer cells MGC-803. By combining single B cell culture with a magnetic bead sorting technology, natural pairing of a heavy chain and a light chain of an antibody can be reserved, and the defect that pairing is damaged by a traditional hybridoma technology is overcome; meanwhile, false positive clones are effectively eliminated through double positive screening of detecting protein binding activity through ELISA and detecting cell binding activity through FACS, the finally obtained targeting 5T4 monoclonal antibody can be specifically combined with human 5T4 protein and tumor cells expressing the protein, and a high-quality targeting molecular basis is provided for research and development of subsequent antibody drugs and CAR-T cell treatment products.

The invention discloses an application of a TaERF9 gene in cooperation with histone modification to participate in nitrogen-mediated root development. The homologous genes of three subgenomes of the TaERF9 gene respectively correspond to the GenBank numbers of CM022223.1, CM022224.1 and CM022225.1 in NCBI (National Center of Biotechnology Information). According to the invention, wheat KN199 is directionally edited by using a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated protein 9) technology, so that a mutant plant with TaERF9 function deletion is obtained. Protein interaction experiments prove that the TaERF9 can interact with the subunit SWN of the PRC2. The results of high-nitrogen and low-nitrogen water culture experiments show that TaERF9 gene mutation significantly promotes root development under a high-nitrogen condition; under the low-nitrogen condition, the root development of the mutant is kept similar to that of a wild type, the ability of balancing the root development under different nitrogen supply conditions is shown, and the effect of the TaERF9 gene in nitrogen response mediated root development regulation is revealed. That is to say, the TaERF9 gene can promote root development after mutation, so that a gene resource is provided for nitrogen-efficient breeding of crops.

The invention discloses an application of protein kinase CPK28 (GenBank: AT5G66210) in endowing or enhancing aluminum resistance of plants, and the CPK28 is overexpressed in the plants, so that a zinc finger transcription factor STOP1 can be phosphorylated, and the protein accumulation of the STOP1 is improved, thereby enabling the plants to generate or enhance the aluminum toxicity resistance. The invention provides a theoretical basis for culturing aluminum toxicity resistant crops.

The application discloses application of a rice metal tolerance protein coding gene OsMTP7 The rice metal tolerance protein coding gene OsMTP7 has the accession number of AK241225.1 in Genbank. The gene coding protein has zinc transport activity, can increase the zinc content of rice root system and above-ground part, and significantly increases the accumulation of zinc in rice grains.

The invention discloses a chloroplast gene ITS2 bar code sequence primer composition, an identification kit and application. The primer composition contains at least one pair of primers which are derived from a nucleotide sequence with the login number of PP277665.1 and / or PP906956.1 from GenBank. The primer composition can effectively identify and / or distinguish patrinia scabiosaefolia, thlaspi arvense and a mixture of the patrinia scabiosaefolia and the thlaspi arvense And the identification method is simple and reliable, has strong objectivity, does not depend on subjective judgment, is compatible with various instruments and reaction systems, is not easily influenced by sample characters, and can be used for accurately identifying the medicinal material powder.

The invention discloses an application of an indica rice OsRNU2 gene. The registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161, and the registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161. The difference of the indica rice OsRNU2 gene promoter is the reason for the utilization efficiency and yield of indica rice and japonica rice nitrogen fertilizer, and the indica rice OsRNU2 gene promoter sequence is shown as SEQ ID NO.2. In the background of Huajing indica 74, 159 parts of materials carrying IRAT261 single fragment replacement lines are used for QTL (quantitative trait loci) analysis, and the site qRNU2 influencing the nitrate nitrogen absorption rate is positioned. The OsRNU2 haplotype expression level in indica rice is low, so that the indica rice can accumulate more auxin. The excellent allelic variation of the OsRNU2 in the indica rice is introduced into the japonica rice, so that the nitrogen fertilizer utilization efficiency and yield of the japonica rice can be improved.

The application discloses application of a rice OsARF24 gene. The nucleotide sequence of the OsARF24 gene has an accession number of LOC4352211 in Genbank. The rice OsARF24 gene is a key element in an auxin signal pathway. Knocking out the OsARF1 can cause the expression amount of nitrogen metabolism related genes of the rice, the nitrate nitrogen absorption rate, the plant height and the yield of the rice to be reduced, and the nitrogen fertilizer utilization efficiency to be reduced in a comprehensive manner. The function of the OsARF24 gene in positively regulating the nitrogen fertilizer utilization efficiency of the rice is clarified, and a technical route for increasing the nitrogen fertilizer utilization efficiency of the rice by increasing the expression of the OsARF24 in the rice is provided.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

This invention relates to a logistics transportation method based on electronic fences, belonging to the field of logistics transportation. The method includes: selecting process DNA matching the logistics task to be processed from a gene bank as parent DNA; performing genetic processing on the parent DNA to obtain multiple offspring DNAs; selecting one of the offspring DNAs as the master DNA based on digital twin technology; generating a dynamic electronic fence based on the master DNA; calculating a deviation vector based on acquired current state data and future state trajectory prediction data; when the deviation vector meets preset conditions, determining the gene segment to be mutated in the master DNA based on the deviation vector and performing mutation processing to obtain multiple variant DNAs; selecting one of the variant DNAs as the optimal variant DNA based on digital twin technology and current state data; updating the master DNA and the dynamic electronic fence based on the optimal variant DNA. This method has the advantages of enabling proactive monitoring and reducing cargo damage and transportation delays.

The application provides a monoclonal antibody for specifically detecting rotavirus and an application thereof, and is directed to epidemic strains of porcine rotavirus, and refers to the gene sequence of VP6 protein published in GenBank, further optimizes an antigen epitope, obtains a purified recombinant VP6 protein with antigenicity, and performs animal immunization, and successfully screens the monoclonal antibody capable of specifically recognizing the porcine rotavirus. The monoclonal antibody screened can effectively recognize the epidemic strains, has the broad-spectrum recognition, is superior to the commercially available antibody, has high antigen recognition target specificity, is low in non-specificity, and has clear sequence background, can be scaled up and produced, and has good commercial value.

The invention discloses a screening method and application of a shark-derived anti-idiotypic nano antibody, and belongs to the technical field of biological pharmacy and vaccines. The screening method comprises the following steps: immunizing adult shark by using a target pathogen specific antigen, collecting peripheral blood, separating lymphocytes, extracting RNA (Ribonucleic Acid), carrying out reverse transcription to synthesize cDNA (Complementary Deoxyribonucleic Acid), amplifying a nano antibody variable region fragment of a shark IgNAR (Immunoglobulin Nucleic Acid Receptor) gene, and constructing a phage display gene bank of which the storage capacity is greater than or equal to 1 * 10 < 8 > CFU and the recombination rate is greater than or equal to 85%; a variable region of a target pathogen specific antibody is taken as a target, specific bacteriophage is enriched through 3-4 rounds of gradient washing and elutriation (Tween-20 concentration and washing time are gradually improved), and the shark source anti-idiotypic nano antibody with high affinity and high purity (greater than or equal to 95%) is obtained by combining ELISA screening positive clone, gene sequencing de-weighting and SPR detection (combining dissociation constant KDlt, 1 * 10 <-7 > mol / L).

The invention relates to the technical field of sea cucumber collagen, in particular to protein with a repairing effect and a preparation method and application thereof. The amino acid sequence of the protein is from Collagen [Apostichopus japonicus] GenBank: APA22677.1 in an NCBI (National Center for Biotechnology Information) database, the strain Pichia sp. With high protein expression quantity is finally found through codon optimization, further expression and screening by virtue of a shake flask and a 10L fermentation tank, the strain Pichia sp. Is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 18, 2025, and the preservation number is CGMCC No.34894. The invention further discloses a preparation method of the Pichia sp. The obtained pure protein has an excellent repairing effect and a certain anti-oxidation effect, and has a great commercial value.

The application discloses an immunoregulatory peptide BugaCATH and application thereof, and belongs to the field of biomedicine. Buga The CATH is a cyclic polypeptide with a pair of intramolecular disulfide bond composed of a sixth cysteine and a thirteenth cysteine, and has a molecular weight of 3156.67 Dalton and an isoelectric point of 8.76, wherein the amino acid sequence is shown as SEQ ID NO: 2. Buga The gene (GenBank accession: OQ870533) of the CATH precursor is composed of 663 nucleotide sequences, and the nucleotide sequence is shown as SEQ ID NO: 1; wherein the nucleotide at the positions of 322-448 is the immunoregulatory peptide Buga The encoding gene of the CATH. The immunoregulatory peptide Buga The application of the CATH in the preparation of a treatment drug for promoting skin wound repair.

The application discloses a kind of energy-saving optimization method and system of manufacturing factory water-cooled air conditioner, it is related to intelligent energy-saving optimization technical field, including collection multimodal data, construct initial gene bank, form cluster gene bank by spectrum decomposition and K-means, using genetic algorithm to update cluster gene bank, output configuration parameter table, set target function and heat balance constraint condition, convert into quadratic binary optimization problem, using quantum annealing algorithm parallel search, by discretization mapping and screening, output initial operation optimization parameter, carry out use and calculate deviation rate, update GP model parameter, output operation optimization parameter.The application forms configuration parameter table by exergy analysis combined with advanced algorithm, improves the accuracy and adaptability of system initial configuration, introduces quantum annealing and GP model dynamic optimization operating parameter, improves the dynamic adaptability and optimization efficiency of operating parameter.

The invention belongs to the technical field of germplasm resource identification, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker combination for identifying germplasm resources of larimichthys crocea 'Ningkang No.1' and application of the SNP molecular marker combination. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for identifying germplasm resources of larimichthys crocea 'Ningkang No.1', the SNP molecular marker combination comprises an SNP site listed in a table 1, and the position and mutation type of the SNP site in a larimichthys crocea reference genome LCFGLHiC1.0 (GENBANK: GCA003845795.1) are shown in the table 1. The SNP molecular marker combination of the Pseudosciaena crocea 'Ningkang No.1' germplasm resource has obvious specificity, can simply and clearly distinguish 'Ningkang No.1' population from other populations by using less genotype information, and provides a scientific and powerful basis for identification and protection of the germplasm resource of the Pseudosciaena crocea 'Ningkang No.1' germplasm resource.

The invention discloses an application of a rice OsRNU9 gene. The registration number of the nucleotide sequence of the OsRNU9 gene in Genbank is LOC4325001, and the registration number of the nucleotide sequence of the OsRNU9 gene in Genbank is LOC4325001. RNA-seq is utilized to discover that the OsRNU9 shows response to the external nitrogen supply level, and fluorescent quantitative PCR (Polymerase Chain Reaction) proves that the OsRNU9 is induced by low nitrogen. Knockout of the OsRNU9 can cause reduction of the nitrogen absorption rate, tillering, plant height and yield of the rice, and the comprehensive performance is that the utilization efficiency of the nitrogen fertilizer is reduced. The function of the OsRNU9 gene for positively regulating the utilization efficiency of the rice nitrogen fertilizer is defined, and a technical route for improving the utilization efficiency of the rice nitrogen fertilizer by increasing expression of OsRNU9 in rice is provided.

The invention belongs to the field of network security, and particularly relates to a malicious code detection system based on an improved Siamese model, which comprises a gene extraction module, a gene pool management module, a Siamese network similarity calculation module, a detection decision module and a gene pool updating module. Performing similarity comparison on the extracted gene sequence and a known malicious code gene pool by utilizing an improved Siamese model to obtain a similarity calculation result; according to a similarity calculation result, judging whether the code file contains malicious gene characteristics or not, and if the malicious code is detected, further determining a malicious code family to which the malicious code belongs; and according to a detection result, updating and optimizing a malicious code gene pool, so that the malicious code gene pool covers the latest malicious code sample and variant features. Through combination of the malicious code gene features and the AI model, the effect of the malicious code detection task is remarkably improved.

The present application relates to the field of plant molecular breeding, and particularly relates to a rice SNP molecular marker and application thereof. The SNP site is located at 10155410bp of the 6th chromosome of rice (GenBank: CP018162.1), and the polymorphism is T or A. The present application designs corresponding LAMP primers for the SNP site, and uses the primers to detect the polymorphism of the SNP site to determine whether the rice has a blast-resistant gene Pigm The SNP molecular marker provided by the present application can effectively identify the blast-resistant gene of rice Pigm The corresponding LAMP primers provided by the present application have the advantages of simple operation, low cost, rapid detection, etc. When applied to molecular marker assisted selection, the breeding of blast-resistant rice varieties can be accelerated, the breeding cycle of blast-resistant rice varieties can be significantly shortened, the breeding cost can be reduced, and the present application has high application value in the field of crop disease prevention.

The invention provides a specific monoclonal antibody for detecting rotavirus and application of the specific monoclonal antibody, aiming at an epidemic strain of porcine rotavirus, reference to a gene sequence of VP6 protein published by GenBank, antigen epitopes are further optimized, and purified recombinant VP6 protein with antigenicity is obtained for animal immunization. The monoclonal antibody capable of specifically recognizing the porcine rotavirus is successfully screened out. The monoclonal antibody obtained through screening can effectively recognize epidemic strains, has broad-spectrum recognition, is superior to commercially available antibodies, and has high antigen recognition target performance and low non-specificity. And the sequence background is clear, large-scale amplification and production can be carried out, and the method has good commercial value.

We describe an antibody or antigen-binding fragment thereof comprising a heavy chain variable region (VH) sequence and a light chain variable region (VL) sequence of a clone selected from: 5C4, 4H1, 1D2, 1C4, 2B5, 1F1, 1H1, 4A5, 1D8, 1D3, 2F1, 3C6, 3D12, 3A9, 1F4, 1H10, 3C12, 4G11, 4A12, 1A12, 3G12, 4F9, 4G4 and 1E1 which is capable of specifically binding to glypican-3 (GPC-3) (GenBank Accession Number: NP_004475.1), or a sequence having at least 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity thereto.

The invention belongs to the technical field of gene engineering, and particularly relates to an application of a BRM gene and an encoding protein thereof in regulating and controlling the acid resistance of plants. And the login number of the BRM gene in a GenBank / EMBL database is AT2G46020. The invention further discloses a method for identifying the BRM gene. The BRM gene can physically interact with a core acid-resistant transcription factor STOP1 to inhibit the transcriptional activation activity of the STOP1 so as to influence the acid resistance of plants. Therefore, after the BRM gene is inhibited, the acid resistance of the plant can be improved, the growth of a plant root system and the absorption of nitrogen nutrition under an acidic condition are promoted, and meanwhile, the alkalization of a rhizosphere environment of the root system is promoted. By interpreting the functions of the BRM gene, an effective gene resource is provided for cultivating acid-resistant plants and improving acid soil while utilizing the acid soil.

The application discloses application of a rice OsRNU6 gene. The nucleotide sequence of the OsRNU6 gene has an accession number of LOC4332833 in Genbank. It is found by RNA-seq that the OsRNU6 shows response to external nitrogen supply level, and it is proved by fluorescence quantitative PCR that the OsRNU6 is induced by low nitrogen. Knocking out the OsRNU6 can cause the nitrogen absorption rate, plant height, tillering and yield of rice to be reduced, and the comprehensive performance is the decrease of nitrogen fertilizer utilization efficiency. The function of the OsRNU6 gene in positively regulating the nitrogen fertilizer utilization efficiency of rice is clarified, and a technical route for improving the nitrogen fertilizer utilization efficiency of rice by increasing the expression of the OsRNU6 in rice is provided.

The present invention discloses the application of the rice OsRNU8 gene. The nucleotide sequence of the OsRNU8 gene is registered in Genbank as LOC4333925. RNA-seq revealed that OsRNU8 responds to external nitrogen supply levels, and fluorescence quantitative PCR demonstrated that OsRNU8 is induced by high nitrogen. Knockout of OsRNU8 results in reduced nitrogen uptake rate, plant height, and yield in rice, which is manifested as a decrease in nitrogen fertilizer use efficiency. The OsRNU8 gene's function in positively regulating rice nitrogen fertilizer use efficiency has been clarified, providing a technical approach for improving rice nitrogen fertilizer use efficiency by increasing the expression of OsRNU8 in rice.

The invention belongs to the technical field of plant genetic engineering and the technical field of soybean breeding, and particularly relates to a ceramide synthetase encoding gene GmCerS1 for regulating and controlling the effective branching number of soybeans and application of the ceramide synthetase encoding gene GmCerS1. The invention discloses the ceramide synthetase encoding gene GmCerS1 for regulating and controlling the effective branching number of soybeans for the first time, the nucleotide sequence of the ceramide synthetase encoding gene GmCerS1 is shown as SEQ ID No.1, and the amino acid sequence of the encoded protein is shown as SEQ ID No.2. The gene is located in an endoplasmic reticulum, and formation and development of lateral branches are promoted by up-regulating expression of genes such as cellulose synthetase, GA20 oxidase and auxin transporter. The effective branch number and pod number of an excessive transgenic plant obtained through genetic transformation are obviously more than those of a wild type. The ceramide synthetase coding gene GmCerS1 for regulating and controlling the soybean effective branch number is disclosed for the first time, a soybean branch regulation and control gene bank is greatly enriched, and a new gene resource is provided for improving soybean effective branch number breeding.

The application relates to the technical field of virus genetic engineering, and particularly relates to a construction method of a porcine respiratory syndrome virus. The construction method is as follows: taking a PRRSV HEB1 strain genome (GenBank EF112447.1) as a template and modifying the same, then cloning the modified genome into a pBAC11 carrier, carrying out in-vitro transcription and transfecting Marc-145 cells, and rescuing a recombinant virus rPRRSV-HA-CMV-mC. The recombinant PRRSV strain shows significant replication delay and titer reduction, forming an attenuated phenotype. Further, the recombinant virus is emulsified with an ISA206 oil adjuvant to prepare a vaccine, which shows high immunogenicity in piglet immunization, can significantly reduce clinical symptoms, viremia and lung pathological damage after PRRSV infection, and has the potential to be developed into a safe and effective vaccine.

The invention provides a C-glycosyl transferase mutant Mut10 with remarkably improved thermal stability and application of the C-glycosyl transferase mutant Mut10. Specifically, a mutant Mut10 of which the thermal stability is greatly improved is obtained by introducing ten-site combined mutation of S155A, V194D, G200Y, L227E, K381P, D411G, G424A, V437R, G456S and C457Q into C-glycosyl transferase GgCGT (GenBank ID: MH998596) from Glycyrrhiza glabra. The invention also comprises a method for producing the heat-resistant C-glycosyl transferase mutant, and an application of the heat-resistant C-glycosyl transferase mutant in catalytic synthesis of phloretin di-Cglucoside.

The present invention relates to the technical field of virus monitoring, and in particular to a method for monitoring respiratory viral infectious diseases, comprising establishing a virus gene library, wherein the virus gene library stores gene sequences of multiple different respiratory viruses; collecting sound information in public places and calculating an environmental risk coefficient, extracting cough events through the sound information, calculating the virus transmission index of the public place, and allocating sampling equipment to the public places in the order of the virus transmission index for sampling; after the sampling is completed, testing the samples, comparing the test results with the gene sequences in the virus gene library to obtain the load of respiratory viruses, and calculating the risk value of the virus; the present invention extracts cough events through sound information, and the cough events can quantify the intensity of virus release behavior in the place, providing a basis for subsequent risk assessment; calculating the environmental risk coefficient and ranking them, allocating and utilizing limited medical resources, and actively sampling in places with a higher probability of transmission.