49 results about "GenBank" patented technology

The invention relates to the technical field of biological medicine, and discloses an anti-human 5T4 single-chain antibody, the antibody has the capability of specifically binding to human 5T4 protein, the human 5T4 protein corresponds to TPBG / WAIF1, and the GenBank login number is NP001018111.1; the antibody also has the capability of specifically binding and expressing human 5T4 protein tumor cells, and the human 5T4 protein tumor cells are breast cancer cells MCF-7, prostate cancer cells PC3 or gastric cancer cells MGC-803. By combining single B cell culture with a magnetic bead sorting technology, natural pairing of a heavy chain and a light chain of an antibody can be reserved, and the defect that pairing is damaged by a traditional hybridoma technology is overcome; meanwhile, false positive clones are effectively eliminated through double positive screening of detecting protein binding activity through ELISA and detecting cell binding activity through FACS, the finally obtained targeting 5T4 monoclonal antibody can be specifically combined with human 5T4 protein and tumor cells expressing the protein, and a high-quality targeting molecular basis is provided for research and development of subsequent antibody drugs and CAR-T cell treatment products.

The invention discloses an application of a TaERF9 gene in cooperation with histone modification to participate in nitrogen-mediated root development. The homologous genes of three subgenomes of the TaERF9 gene respectively correspond to the GenBank numbers of CM022223.1, CM022224.1 and CM022225.1 in NCBI (National Center of Biotechnology Information). According to the invention, wheat KN199 is directionally edited by using a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated protein 9) technology, so that a mutant plant with TaERF9 function deletion is obtained. Protein interaction experiments prove that the TaERF9 can interact with the subunit SWN of the PRC2. The results of high-nitrogen and low-nitrogen water culture experiments show that TaERF9 gene mutation significantly promotes root development under a high-nitrogen condition; under the low-nitrogen condition, the root development of the mutant is kept similar to that of a wild type, the ability of balancing the root development under different nitrogen supply conditions is shown, and the effect of the TaERF9 gene in nitrogen response mediated root development regulation is revealed. That is to say, the TaERF9 gene can promote root development after mutation, so that a gene resource is provided for nitrogen-efficient breeding of crops.

The invention discloses an application of protein kinase CPK28 (GenBank: AT5G66210) in endowing or enhancing aluminum resistance of plants, and the CPK28 is overexpressed in the plants, so that a zinc finger transcription factor STOP1 can be phosphorylated, and the protein accumulation of the STOP1 is improved, thereby enabling the plants to generate or enhance the aluminum toxicity resistance. The invention provides a theoretical basis for culturing aluminum toxicity resistant crops.

The application discloses application of a rice metal tolerance protein coding gene OsMTP7 The rice metal tolerance protein coding gene OsMTP7 has the accession number of AK241225.1 in Genbank. The gene coding protein has zinc transport activity, can increase the zinc content of rice root system and above-ground part, and significantly increases the accumulation of zinc in rice grains.

The invention discloses a chloroplast gene ITS2 bar code sequence primer composition, an identification kit and application. The primer composition contains at least one pair of primers which are derived from a nucleotide sequence with the login number of PP277665.1 and / or PP906956.1 from GenBank. The primer composition can effectively identify and / or distinguish patrinia scabiosaefolia, thlaspi arvense and a mixture of the patrinia scabiosaefolia and the thlaspi arvense And the identification method is simple and reliable, has strong objectivity, does not depend on subjective judgment, is compatible with various instruments and reaction systems, is not easily influenced by sample characters, and can be used for accurately identifying the medicinal material powder.

The application discloses application of a rice OsARF24 gene. The nucleotide sequence of the OsARF24 gene has an accession number of LOC4352211 in Genbank. The rice OsARF24 gene is a key element in an auxin signal pathway. Knocking out the OsARF1 can cause the expression amount of nitrogen metabolism related genes of the rice, the nitrate nitrogen absorption rate, the plant height and the yield of the rice to be reduced, and the nitrogen fertilizer utilization efficiency to be reduced in a comprehensive manner. The function of the OsARF24 gene in positively regulating the nitrogen fertilizer utilization efficiency of the rice is clarified, and a technical route for increasing the nitrogen fertilizer utilization efficiency of the rice by increasing the expression of the OsARF24 in the rice is provided.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

This invention relates to a logistics transportation method based on electronic fences, belonging to the field of logistics transportation. The method includes: selecting process DNA matching the logistics task to be processed from a gene bank as parent DNA; performing genetic processing on the parent DNA to obtain multiple offspring DNAs; selecting one of the offspring DNAs as the master DNA based on digital twin technology; generating a dynamic electronic fence based on the master DNA; calculating a deviation vector based on acquired current state data and future state trajectory prediction data; when the deviation vector meets preset conditions, determining the gene segment to be mutated in the master DNA based on the deviation vector and performing mutation processing to obtain multiple variant DNAs; selecting one of the variant DNAs as the optimal variant DNA based on digital twin technology and current state data; updating the master DNA and the dynamic electronic fence based on the optimal variant DNA. This method has the advantages of enabling proactive monitoring and reducing cargo damage and transportation delays.

The application provides a monoclonal antibody for specifically detecting rotavirus and an application thereof, and is directed to epidemic strains of porcine rotavirus, and refers to the gene sequence of VP6 protein published in GenBank, further optimizes an antigen epitope, obtains a purified recombinant VP6 protein with antigenicity, and performs animal immunization, and successfully screens the monoclonal antibody capable of specifically recognizing the porcine rotavirus. The monoclonal antibody screened can effectively recognize the epidemic strains, has the broad-spectrum recognition, is superior to the commercially available antibody, has high antigen recognition target specificity, is low in non-specificity, and has clear sequence background, can be scaled up and produced, and has good commercial value.

The invention discloses a screening method and application of a shark-derived anti-idiotypic nano antibody, and belongs to the technical field of biological pharmacy and vaccines. The screening method comprises the following steps: immunizing adult shark by using a target pathogen specific antigen, collecting peripheral blood, separating lymphocytes, extracting RNA (Ribonucleic Acid), carrying out reverse transcription to synthesize cDNA (Complementary Deoxyribonucleic Acid), amplifying a nano antibody variable region fragment of a shark IgNAR (Immunoglobulin Nucleic Acid Receptor) gene, and constructing a phage display gene bank of which the storage capacity is greater than or equal to 1 * 10 < 8 > CFU and the recombination rate is greater than or equal to 85%; a variable region of a target pathogen specific antibody is taken as a target, specific bacteriophage is enriched through 3-4 rounds of gradient washing and elutriation (Tween-20 concentration and washing time are gradually improved), and the shark source anti-idiotypic nano antibody with high affinity and high purity (greater than or equal to 95%) is obtained by combining ELISA screening positive clone, gene sequencing de-weighting and SPR detection (combining dissociation constant KDlt, 1 * 10 <-7 > mol / L).

The application discloses an immunoregulatory peptide BugaCATH and application thereof, and belongs to the field of biomedicine. Buga The CATH is a cyclic polypeptide with a pair of intramolecular disulfide bond composed of a sixth cysteine and a thirteenth cysteine, and has a molecular weight of 3156.67 Dalton and an isoelectric point of 8.76, wherein the amino acid sequence is shown as SEQ ID NO: 2. Buga The gene (GenBank accession: OQ870533) of the CATH precursor is composed of 663 nucleotide sequences, and the nucleotide sequence is shown as SEQ ID NO: 1; wherein the nucleotide at the positions of 322-448 is the immunoregulatory peptide Buga The encoding gene of the CATH. The immunoregulatory peptide Buga The application of the CATH in the preparation of a treatment drug for promoting skin wound repair.

The application discloses a kind of energy-saving optimization method and system of manufacturing factory water-cooled air conditioner, it is related to intelligent energy-saving optimization technical field, including collection multimodal data, construct initial gene bank, form cluster gene bank by spectrum decomposition and K-means, using genetic algorithm to update cluster gene bank, output configuration parameter table, set target function and heat balance constraint condition, convert into quadratic binary optimization problem, using quantum annealing algorithm parallel search, by discretization mapping and screening, output initial operation optimization parameter, carry out use and calculate deviation rate, update GP model parameter, output operation optimization parameter.The application forms configuration parameter table by exergy analysis combined with advanced algorithm, improves the accuracy and adaptability of system initial configuration, introduces quantum annealing and GP model dynamic optimization operating parameter, improves the dynamic adaptability and optimization efficiency of operating parameter.

The invention belongs to the technical field of germplasm resource identification, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker combination for identifying germplasm resources of larimichthys crocea 'Ningkang No.1' and application of the SNP molecular marker combination. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for identifying germplasm resources of larimichthys crocea 'Ningkang No.1', the SNP molecular marker combination comprises an SNP site listed in a table 1, and the position and mutation type of the SNP site in a larimichthys crocea reference genome LCFGLHiC1.0 (GENBANK: GCA003845795.1) are shown in the table 1. The SNP molecular marker combination of the Pseudosciaena crocea 'Ningkang No.1' germplasm resource has obvious specificity, can simply and clearly distinguish 'Ningkang No.1' population from other populations by using less genotype information, and provides a scientific and powerful basis for identification and protection of the germplasm resource of the Pseudosciaena crocea 'Ningkang No.1' germplasm resource.

The invention discloses an application of a rice OsRNU9 gene. The registration number of the nucleotide sequence of the OsRNU9 gene in Genbank is LOC4325001, and the registration number of the nucleotide sequence of the OsRNU9 gene in Genbank is LOC4325001. RNA-seq is utilized to discover that the OsRNU9 shows response to the external nitrogen supply level, and fluorescent quantitative PCR (Polymerase Chain Reaction) proves that the OsRNU9 is induced by low nitrogen. Knockout of the OsRNU9 can cause reduction of the nitrogen absorption rate, tillering, plant height and yield of the rice, and the comprehensive performance is that the utilization efficiency of the nitrogen fertilizer is reduced. The function of the OsRNU9 gene for positively regulating the utilization efficiency of the rice nitrogen fertilizer is defined, and a technical route for improving the utilization efficiency of the rice nitrogen fertilizer by increasing expression of OsRNU9 in rice is provided.

The present application relates to the field of plant molecular breeding, and particularly relates to a rice SNP molecular marker and application thereof. The SNP site is located at 10155410bp of the 6th chromosome of rice (GenBank: CP018162.1), and the polymorphism is T or A. The present application designs corresponding LAMP primers for the SNP site, and uses the primers to detect the polymorphism of the SNP site to determine whether the rice has a blast-resistant gene Pigm The SNP molecular marker provided by the present application can effectively identify the blast-resistant gene of rice Pigm The corresponding LAMP primers provided by the present application have the advantages of simple operation, low cost, rapid detection, etc. When applied to molecular marker assisted selection, the breeding of blast-resistant rice varieties can be accelerated, the breeding cycle of blast-resistant rice varieties can be significantly shortened, the breeding cost can be reduced, and the present application has high application value in the field of crop disease prevention.

The invention provides a specific monoclonal antibody for detecting rotavirus and application of the specific monoclonal antibody, aiming at an epidemic strain of porcine rotavirus, reference to a gene sequence of VP6 protein published by GenBank, antigen epitopes are further optimized, and purified recombinant VP6 protein with antigenicity is obtained for animal immunization. The monoclonal antibody capable of specifically recognizing the porcine rotavirus is successfully screened out. The monoclonal antibody obtained through screening can effectively recognize epidemic strains, has broad-spectrum recognition, is superior to commercially available antibodies, and has high antigen recognition target performance and low non-specificity. And the sequence background is clear, large-scale amplification and production can be carried out, and the method has good commercial value.

We describe an antibody or antigen-binding fragment thereof comprising a heavy chain variable region (VH) sequence and a light chain variable region (VL) sequence of a clone selected from: 5C4, 4H1, 1D2, 1C4, 2B5, 1F1, 1H1, 4A5, 1D8, 1D3, 2F1, 3C6, 3D12, 3A9, 1F4, 1H10, 3C12, 4G11, 4A12, 1A12, 3G12, 4F9, 4G4 and 1E1 which is capable of specifically binding to glypican-3 (GPC-3) (GenBank Accession Number: NP_004475.1), or a sequence having at least 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity thereto.

The invention provides a C-glycosyl transferase mutant Mut10 with remarkably improved thermal stability and application of the C-glycosyl transferase mutant Mut10. Specifically, a mutant Mut10 of which the thermal stability is greatly improved is obtained by introducing ten-site combined mutation of S155A, V194D, G200Y, L227E, K381P, D411G, G424A, V437R, G456S and C457Q into C-glycosyl transferase GgCGT (GenBank ID: MH998596) from Glycyrrhiza glabra. The invention also comprises a method for producing the heat-resistant C-glycosyl transferase mutant, and an application of the heat-resistant C-glycosyl transferase mutant in catalytic synthesis of phloretin di-Cglucoside.

The application discloses a kind of, the application can respond to serine and homoserine derived from glutamic acid coryneform 13032 NCgl0581 transcriptional regulator (GenBank: BAB97999.1) F104 position is saturated mutation, to this as foundation biological element, constructs a kind of high-efficiency amino acid biosensor, then the response amino acid concentration is coupled with fluorescence intensity signal, realizes the real-time monitoring of strain intracellular response amino acid concentration.The mutant not only has the induction response activity of serine and homoserine, and also has the induction response activity of proline and threonine.It can be applied to the genetic modification and screening of threonine and proline high-yield strain.

The application discloses a method for detecting viruses and virus replication in media based on Small-RNA high-throughput sequencing. The application belongs to the technical field of virus detection, and the method for identifying virus types in a to-be-detected sample by Small-RNA sequencing developed by the application comprises the following steps: 1) extracting total RNA from a virus-infected to-be-detected sample, constructing a cDNA library, and separating small RNA sequence fragments from the cDNA library; 2) downloading virus sequences and classification databases from GenBank, and comparing the small RNA sequence fragments with virus sequence files; 3) comparing a contigs3 file with a virus nucleic acid database, selecting sequences with the minimum e value in the comparison results in the contigs3 file, calculating the coverage of the contigs3 sequences in corresponding virus genomes, distinguishing virus information with a coverage threshold, and deducing the virus source of the small RNA sequence.

The invention discloses a porcine astrovirus type 5 TaqMan fluorescent quantitative PCR (polymerase chain reaction) detection method, and relates to the technical field of biological detection.The method comprises the following steps: designing specific primers and a TaqMan probe: designing and synthesizing the specific primer pair and the TaqMan probe according to a gene sequence of the porcine astrovirus type 5 in Genbank; sample pretreatment: pretreating a to-be-detected pig manure sample or intestinal tract sample to obtain a supernatant of to-be-extracted nucleic acid; virus nucleic acid extraction: extracting virus RNA (Ribonucleic Acid) in the sample by adopting a column extraction method; construction of a fluorescent quantitative PCR reaction system: taking the extracted virus RNA as a template, adding the specific primer, a TaqMan probe and a fluorescent quantitative PCR reaction related reagent, and constructing the reaction system; fluorescent quantitative PCR amplification: setting a specific amplification program to carry out PCR reaction, and monitoring a fluorescent signal in real time; and result judgment: judging whether the sample contains the porcine astrovirus type 5 nucleic acid or not according to the amplification curve, the Ct value and the standard curve.

The invention relates to the field of plant molecular breeding, in particular to a rice SNP (Single Nucleotide Polymorphism) molecular marker and application thereof. The SNP locus is located at the 10155410bp position (GenBank: CP018162.1) of the No.6 chromosome of the rice, and the polymorphism of the SNP locus is T or A. The invention further discloses the application of the SNP locus. A corresponding LAMP primer is designed aiming at the SNP site, and the primer is used for detecting the polymorphism of the SNP site to judge whether the rice has the rice blast resistance gene Pigm or not. The SNP molecular marker provided by the invention can be used for effectively identifying the rice blast resistance gene Pigm. The corresponding LAMP primer provided by the invention has the advantages of simplicity in operation, low cost, rapidness in detection and the like, can be applied to molecular marker-assisted selection to accelerate the cultivation of the rice blast-resistant rice variety, remarkably shorten the breeding period of the rice blast-resistant rice variety and reduce the breeding cost, and has relatively high application value in the field of crop disease control.

The invention discloses application of a hippophae rhamnoides Hgfw3.2 gene in regulation and control of fruit size, and belongs to the technical field of biological genetic engineering. The nucleotide sequence of the Hgfw3.2 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the Hgfw3.2 gene is as shown in SEQ ID NO. 2. According to the invention, the key gene Hgfw3.2 for regulating and controlling the fruit size is cloned and identified from sea-buckthorn in Jiangcumin for the first time, and the positive regulation and control function of the gene in fruit development is defined. Through overexpression of the Hgfw3.2 gene in a tomato system, the weight, the longitudinal diameter and the transverse diameter of a fruit are obviously increased, the volume of the fruit is obviously increased, and the promotion effect of the gene on the size of the fruit is proved. Compared with the prior art, the invention provides a new gene resource from an extreme environment adaptive plant sea-buckthorn, enriches a gene pool for regulating and controlling the fruit size, and provides a new molecular target for improving the fruit size.

The application discloses a method for identifying duck palm weight and a related molecular marker application, relates to the technical field of molecular marker assisted selection, and a primer pair is used for amplifying a DNA fragment containing a polymorphic site of a duck reference genome IASCAAS_PekinDuck_T2T chromosome 4 from the 5' end 63823029 base position; the duck reference genome IASCAAS_PekinDuck_T2T is a duck reference genome sequence in a GenBank database; the duck is a Zhongxinbaihu meat duck, and the primer pair consists of a DNA molecule shown in SEQ ID No. 2 and a DNA molecule shown in SEQ ID No. 3. The method for identifying the duck palm weight and the related molecular marker application can determine the genotype of the duck by detecting the genomic DNA of the duck, realize early in-vivo screening of the duck palm weight trait, do not need to wait for post-slaughter determination of a phenotype, significantly shorten a breeding cycle, and reduce breeding cost.

This invention discloses the expression of plastic depolymerase LCC. ICCG The recombinant engineered bacteria, their construction method, and applications are disclosed. The recombinant engineered bacteria use *Pichia pastoris* as the host strain and co-express the plastic-degrading enzyme LCC. ICCG and molecular chaperones; the molecular chaperone is endoplasmic reticulum oxidoreductase ERO1; or a combination of transcription factor HAC1 and endoplasmic reticulum oxidoreductase ERO1; the plastic depolymerase LCC ICCG The GenBank accession number is ON529840.1. The LCC of this engineered bacterium... ICCG The enzyme secretion and expression levels were significantly increased, providing a basis for the development of plastic depolymerase LCC. ICCG This provides a feasible strain product for large-scale preparation and industrial application.

The present application relates to a kind of abdominal spot frog antibacterial peptide Nv-CATH and its gene and application, the antibacterial peptide Nv-CATH is the circular polypeptide coded by the defense peptide gene of amphibian species unique species abdominal spot frog in China, molecular weight 3356.98 Dalton, isoelectric point 11.56, its amino acid sequence is as shown in SEQ ID NO:1.The gene (GenBank accession No.OP264074) of the precursor of the abdominal spot frog antibacterial peptide Nv-CATH is composed of 714 nucleotide sequences, its nucleotide sequence is as shown in SEQ ID NO:2, wherein the 373-522th nucleotide is the encoding gene of mature abdominal spot frog antibacterial peptide Nv-CATH.The application of abdominal spot frog antibacterial peptide Nv-CATH in preparing the therapeutic drug of the infection disease caused by hemolytic staphylococcus, enterococcus faecalis, staphylococcus aureus, klebsiella pneumoniae, acinetobacter junni and / or paratyphi A.The present application provides a new antibacterial peptide Nv-CATH with strong broad-spectrum antibacterial activity.

The application discloses an immunomodulatory peptide Ha-CATH and application thereof, and belongs to the field of biomedicine. The immunomodulatory peptide Ha-CATH is a cyclic polypeptide with a pair of intramolecular disulfide bonds formed by the eighth cysteine residue and the thirteenth cysteine residue of a skin repair peptide gene of Chinese amphibian Pelophylax nigromaculatus, has a molecular weight of 2361.8 Dalton, an isoelectric point of 6.04, and an amino acid sequence shown in SEQ ID NO:1. A gene (GenBank accession: OQ992774) encoding the precursor of the immunomodulatory peptide Ha-CATH is composed of 714 nucleotides, has a nucleotide sequence shown in SEQ ID NO:2, and the nucleotide sequence of 445-504 is the encoding gene of the immunomodulatory peptide Ha-CATH. ‑ The application also discloses an application of the immunomodulatory peptide Ha-CATH in preparation of a skin wound repair promoting therapeutic drug. ‑ The application also discloses an application of the immunomodulatory peptide Ha-CATH in preparation of a skin wound repair promoting therapeutic drug.

This invention relates to the field of ornamentation gene bank construction technology, specifically disclosing a method and system for constructing an ornamentation gene bank based on visual information. The method includes acquiring a surface map for ornamentation analysis; performing ornamentation feature analysis on the surface map, dividing the surface map into regions based on the ornamentation features, and intelligently recognizing each region to obtain corresponding ornamentation recognition results; constructing sample data corresponding to the sample based on the region division results and ornamentation recognition results, and constructing an ornamentation gene bank; and providing guidance information for the ornamentation gene bank generation design process. This invention uses AI to recognize the surface of sample models, obtain recognition results, classify samples according to the recognition process and results, determine the importance of each sample based on the classification results, and thus determine the information acquisition order, allowing information from more important samples to be acquired first, thereby indirectly improving the data quality of the gene bank and enabling it to be put into use earlier.