915 results about "GenBank" patented technology

The present invention relates to a functionally expressed bacterial voltage-sensitive ion-selective channel. The novel channel can be used in conjunction with mammalian ion channel pore-containing regions in assays for screening for compounds that modulate activity of the channel. One bacterial ion channel of the present invention, called NaChBac, is derived from Bacillus halodurans (GenBank#BAB05220 (8)).

The invention relates to an agrobacterium strain (Agrobacterium sp.) with heterotrophic nitrification-aerobic denitrification capability and an application thereof in nitrogenous effluent treatment. The bacterial strain is characterized in that: 16S rRNA gene contains nucleotide sequence shown in sequence table with a sequence length of 1418bp and a accession number FJ639330 in Genbank. The preservation number thereof is CGMCC No.2962. The agrobacterium of the invention has not only heterotrophic nitrification capability but also aerobic denitrification capability. During nitrogenous effluent treatment, and one aerobic phase can cause ammonian to change into gaseous product with high denitrification efficiency and easy operation and tremendous economic benefit compared with the traditional biological denitrification process.

De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

Libraries of modified AAV cap genes are generated by synthesizing modified cap genes using degenerate oligonucleotides. Combinatorial libraries of virions composed of modified (e.g., chimeric), replication-competent AAV vectors and modified Cap proteins are also produced. Helper vectors are described that encode AAV Rep protein, modified Cap proteins, and Ad proteins. The helper vectors are used to produce stocks of virions composed of modified (e.g., chimeric) capsids and rAAV vectors.

The present invention relates to an in vitro method for diagnosing and/or monitoring in a subject a gastrointestinal stromal tumor or a predisposition to develop a gastrointestinal stromal tumor, comprising detecting and/or analyzing in a test sample derived from the subject one or more mutations at the DNA level in any one or both of the marker genes cKIT (GenBank acc. no. NM_000222.2) and PDGFRA (GenBank acc. no. NM_006206.4), wherein the DNA is circulating DNA, and wherein the presence of any one of the mutations detected in the test sample is indicative of a gastrointestinal stromal tumor or a predisposition to develop a gastrointestinal stromal tumor in the subject. The present invention is also directed to a corresponding kit-of-parts for diagnosing and/or monitoring a gastrointestinal stromal tumor or a predisposition to develop a gastrointestinal stromal tumor, comprising means for detecting and/or analyzing one or more mutations as defined herein, as well as to the use of one or more mutations as defined herein as a panel of molecular markers for diagnosing and/or monitoring a gastrointestinal stromal tumor or a predisposition to develop a gastrointestinal stromal tumor.

Roughly described, a computer-implemented evolutionary data mining system includes a memory storing a candidate gene database in which each candidate individual has a respective fitness estimate; a gene pool processor which tests individuals from the candidate gene pool on training data and updates the fitness estimate associated with the individuals in dependence upon the tests; and a gene harvesting module providing for deployment selected ones of the individuals from the gene pool, wherein the gene pool processor includes a competition module which selects individuals for discarding from the gene pool in dependence upon both their updated fitness estimate and their testing experience level. Preferably the gene database has an elitist pool containing multiple experience layers, and the competition module causes individuals to compete only with other individuals in their same experience layer.

The invention discloses a genetically engineered bacteria used for producing stevia glycosyltransferase UGT76G1; a UGT76G1 coding gene is inserted between the restriction enzyme cutting sites EcoRI and XhoI of a PYes2 carrier, so as to construct a recombinant plasmid, and then the recombinant plasmid is introduced to expression host Saccharomyces cerevisiae YPH499 to obtain the engineered bacteria; and the coding gene of the UGT76G1 is GenBank, No. GenBank: AY345974.1, and the gene sequence of the coding gene is named as UGT (Udp Glucuronyl Transferase). The invention also discloses a construction method of the genetically engineered bacteria and the application of the bacteria to the production of rebaudioside A. According to the invention, under the condition that expensive UDPG (Uridine Diphosphate Glucose) is not added, cheap carbon source glucose is used as a substrate, the metabolic pathway of UDPG in the yeast is regulated, and then the rebaudioside A is produced from St glycosides through whole cell catalysis.

The invention relates to an environment DNA identification method for fish community structure researching. The method is characterized by comprising the steps that 1, water samples are collected according to the size of the water area where a fish community is located; 2, the water samples are processed, and total environment DNA of the processed water samples is extracted; 3, a 16s r DNA sequence with nucleotide sequences being SEQ ID NO:1 and SEQ ID NO:2 is adopted as a universal primer to carry out PCR amplification on the total environment DNA, and a PCR product is obtained; 4, gel electrophoresis is carried out on the PCR product, and gel with DNA is obtained; 5, gel cutting and clone sequencing are carried out on the gel with the DNA, and then blast comparison is carried out through GENBANK so as to determine whether the DNA is a fish sequence or not; 6, after it is determined that the DNA is the fish sequence, the next-generation sequencing technology is adopted for analyzing composition conditions of the PCR product on a large scale, and therefore the environment DNA fish composition and community structure are determined. The environment DNA identification method is quite easy, convenient and efficient and has the practical value.

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of vibrio parahemolyticus can augment the specific base sequence of a target gene which is the tih-GenBank (accession no. AY578148) of the vibrio parahemolyticus, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 946-1140 loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the vibrio parahemolyticus, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the vibrio parahemolyticus, determines whether the vibrio parahemolyticus exists in the specimen or not.

The invention relates to a bacterial strain for degrading zearalenone toxin, which is characterized in that: the preserving number of the bacterial strain is CCTCC NO: M2010053; and the Genbank accession number of the bacterial strain 16S rDNA is HM372880. When in application, the bacterial strain is prepared into liquid bactericide or solid bactericide which can degrade the zearalenone toxin for degrading the zearalenone toxin in grains or fodders. The bacterial strain has the advantages that: the process of using the bacterial strain to degrade the zearalenone toxin is free of secondary pollution, thereby improving the quality of agricultural products and ensuring the edible safety of people and livestock; and the bacterial strain is used for producing various preparations which are used for degrading the zearalenone toxin the bacterial strain, which is not only low in production and using cost, but also safe in use.

A search of the public human genome database identified a human EST, GenBank accession number AW293249, which has high homology to known pufferfish urocortin sequences. The full length sequence was amplified from human genomic DNA and sequenced. Sequence homology comparisons of the novel sequence with human urocortin I and urocortin II revealed that the sequence encoded a novel human urocortin, which was designated urocortin III (UcnIII). While urocortin III does not have high affinity for either CRF-R1 or CRF-R2, the affinity for CRF-R2 is greater than the affinity for CRF-R1. Urocortin III is capable stimulating cyclic AMP production in cells expressing CRF-R2α or β. Thus, the affinity is high enough that urocortin III could act as a native agonist of CRF-R2. However, it is also likely that urocortin III is a stronger agonist of a yet to be identified receptor.

The invention discloses a PCR primer for detecting an African swine fever virus, a kit and application thereof, and belongs to the detection field of the African swine fever virus. According to a conserved region of an ASFV p72 gene on the GenBank, four pairs of primers are designed, the primer with strong specificity and high sensitivity is screened out from the four pairs of primers, and the primer is composed of nucleotide sequences as shown in SEQ ID No.1 and SEQ ID No.2. The invention further discloses a kit prepared from the primer and used for detecting the African swine fever virus, and a corresponding PCR detection method is established. The detection method established by the invention is more specific and sensitive in comparison with two African swine fever virus PCR detection methods recommended by OIE; and the clinical sample detection result shows that the PCR detection method disclosed by the invention is simple in operation, low in cost, good in specificity and high in sensitivity, and can be effectively applied to the screening and fast diagnosis of the African swine fever.

The invention discloses variant porcine reproductive and respiratory syndrome virus (PRRSV) TaqMan fluorescence quantitative RT-PCR detecting kit and application thereof. A primer and a TaqMan probe are designed and synthesized by referring to an NSP2 fragment gene sequence of the variant PRRSV and common PRRSV of a GenBank. By optimizing the reaction condition and constructing a standard plasmid product, a method for diagnosing the variant PRRSV by TaqMan fluorescence quantitative RT-PCR is established. A result indicates that the method has the advantages of strong specificity, high sensitivity, and the like and can detect the standard plasmid product with 264 copy numbers, and the virus quantity of 0.5623TICD50 is 10 times more sensitive than RT-PCR. By detecting 22 disease samples, 8 disease samples are positive, and the positive rate is 36.4 percent. Because the method has the advantages of quantification, high speed, accuracy, sensitivity, and the like, the invention is suitable for the diagnosis on the swinery infected variant PRRSV in the early stage, the medium stage and the later stage and plays an important role in effectively diagnosing, preventing and treating the highly pathogenic PRRSV.

The invention discloses an E.tenella protein disulfide linkage isomerase gene EtPDI (Clone ID is BW1-E06,and the Genbank accession number of is EF552214). The gene is connected with a procaryon expression vector pGEX-4T-2; a procaryon expression recombination plasmid pGEX-4T-EtPDI is constructed and is expressed in a colibacillus system; and most of the expressed recombining protein exists in a soluble form. The recombining protein 4T-EtPDI is purified to carry out SPS-PAGE and is transferred to a PVDF film; and antiserum of E.tenella oocyst oral immunized chicken is used as first resistance and goat anti-chicken IgG is used as second resistance to carry out Western-blot analysis, thereby indicating that the gene has certain antigen. The gene is used for preparing an anti-chicken coccidiosis drug and an anti-chicken coccidiosis vaccine.

The invention provides a dual fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) specific primer for detecting type 1 and type 3 duck hepatitis A viruses. A dual fluorescence quantitative method comprises the following steps: comparing sequences of DHAV-1 and DHAV-3 published on GenBank, and designing a pair of specific detection primers SEQ1 and SEQ2 aiming at DHAV-1 and a Taqman probe (probe1) as well as a pair of specific detection primers SEQ3 and SEQ4 aiming at DHAV-3 and a Taqman probe (probe2) respectively in a region with conservation and relatively large difference between gene sequences of the two viruses; confirming the concentration of the dual fluorescence quantitative RT-PCR specific primer and the Taqman probe aiming at DHAV-1 and DHAV-3. The dual fluorescence quantitative method built by virtue of the group of primers is good in specificity and high in sensitivity, and can be used for quick serum type identification and real-time quantitative analysis of the type 1 and type 3 duck hepatitis A viruses.

The invention relates to bacillus capable of efficiently degrading an organophosphorus pesticide and separated from rape phyllospheres, and provides a method for preparing a microbial inoculum thereof, which belong to the field of biotechnology. The bacillus is identified as bacillus subtilis, which is preserved in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, wherein the preservation No. is CGMCC No. 3374; the 16S rDNA gene sequence of the bacillus strain is submitted to a GenBank database; and the accession number is GU086422. The method for producing the microbial inoculum comprises the following 5 steps of: the preparation of a culture medium, the activation of a bacterium strain, the preparation of a liquid seed, the liquid state fermentation and the preparation of the microbial inoculum, wherein the fermentation culture medium takes molasses as a main raw material. Because the organophosphorus pesticide degrading microbial inoculum prepared by the technical scheme of the invention can efficiently degrade the organophosphorus pesticide residue on plant leaves in situ, the microbial inoculum can guarantee the safety of foods and protect the ecological environment.

A rapid molecular detection method of microbial community composition in a wastewater biological treatment reactor includes: collecting the microorganism samples in the wastewater biological treatment reactor for pretreatment, and using the phenol-chloroform method for extracting total genomic DNA; using 16S rDNAV3 area primers to perform PCR amplification to the sample DNA; using denaturing gradient gel electrophoresis to analyzing the PCR amplification products; using software to analyze the obtained DGGE patterns, and identifying the represented microorganism based on the gel cutting sequencing feature DGGE bands; designing and constructing denaturing gradient gel electrophoresis database as the contradistinguish standard of rapid molecular detection based on the microorganism kinds represented by bands, wherein, the denaturing gradient gel electrophoresis integrated database is designed and constructed by gel cutting cloning sequencing the bands after the PCR products is processed after the DGGE separation and then defining the represented microorganism kinds after consanguinity comparison in the Genbank.

The invention discloses a bacterial nucleic acid sequencing identification method based on a DNA bar code. Common bacterial contaminants in a medicine production environment can be effectively and accurately identified. The bacterial nucleic acid sequencing identification method comprises the steps that 1, a monoclonal bacterial strain is obtained through culture; 2, a genome DNA of the monoclonal bacterial strain obtained in the step 1 is extracted; 3, PCR is performed by using a general nucleic acid sequencing primer to amplify a specific sequence of nucleic acids; 4, a PCR product is extracted and purified; 5, a nucleic acid sequence of the PCR product obtained in the step 4 is determined; 6, a sequence analysis software is applied to perform sequence splicing, positioning is performed by using a general nucleic acid sequencing primer pair, a primer region is removed, and a DNA sequence of a corresponding fragment is obtained; 7, the DNA sequence of the corresponding fragment in the step is imported into a DNA bar code standard sequence core database or a GenBank database for sequence comparison, and bacteria are identified.

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of comma bacillus can augment the specific base sequence of a target gene which is the ompW-GenBank (accession no. AF001009) of the comma bacillus, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 14-220 loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the comma bacillus, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the comma bacillus, determines whether the comma bacillus exists in the specimen or not.