Kit for detecting GII type norovirus and applications thereof

A kit and virus technology, applied in the field of molecular biology and virus detection, can solve the problems of low accuracy and sensitivity, difficulty in improving detection speed, precision and expensive instruments, etc., achieve low reaction temperature, constant reaction temperature, and short detection time Effect

Inactive Publication Date: 2013-11-20
SHANGHAI OCEAN UNIV
View PDF4 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain-type specificity of the ELISA detection method is too strong, the accuracy and sensitivity are low, and the detection is unstable, so the application range is relatively narrow, and it is difficult to meet the requirements of modern detection and diagnosis technology.
Although the PCR method (including RT-PCR and Real-time RT-PCR) makes up for the shortcomings of ELISA, the main technical factor that limits its application is that the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting GII type norovirus and applications thereof
  • Kit for detecting GII type norovirus and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 Design and verification of primers and probes of the present invention

[0020] 1. Experimental materials

[0021] Reverse transcriptase and 1×RT reaction buffer, produced by Roche; RNaseOut inhibitor, produced by Invitrogen; TwistAmp DNA amplification kit produced by TwistDx, including Rehydration Buffer reaction buffer solution, DNA recombinase, DNA polymerase, single Chain binding protein, phosphokinase, ATP and other reagents, among which DNA recombinase, DNA polymerase, single chain binding protein, phosphokinase, ATP and other reagents are stored in the reaction centrifuge tube in the form of dry powder.

[0022] 2. Experimental method

[0023] 2.1 Design of primers and probes

[0024] According to the target sequence of Norovirus type GII (NCBI accession number: X86557), the following primers and probes were designed to specifically recognize the target sequence:

[0025] Forward primer (FP2): 5'-TGGATGAGATTCTCAGATCTGAGCACGTGGGAGGG-3' (SEQ ID NO...

Embodiment 2

[0046] Embodiment 2 Kit 1 of the present invention

[0047] The kit contains the following reagents and materials:

[0048] Primer solution: forward primer FP2 (SEQ ID NO.1, 5 μmol / L); reverse primer RP3 (SEQ ID NO.2, 5 μmol / L).

Embodiment 3

[0049] Embodiment 3 Kit 2 of the present invention

[0050] The kit contains the following reagents and materials:

[0051] Primer solution: forward primer FP2 (SEQ ID NO.1, 5 μmol / L); reverse primer RP3 (SEQ ID NO.2, 5 μmol / L); probe (SEQ ID NO.3, 5 μmol / L).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a kit for detecting GII type norovirus and applications thereof, concretely a primer pair and a probe for detecting the GII type norovirus, and a kit comprising the primer pair and the probe. The GII type norovirus in a sample cab be successfully detected by a recombinase polymerase amplification reaction, and can be quantified by fluorescence intensity. The kit has advantages of high sensitivity, strong singularity, short detection time, and constant and low reaction temperature, can be used for qualitative screening and quantitative determination for GII type norovirus in the sample, such as a clinic sample, foodstuff, an aquatic product, a water body, etc. accordingly, the kit can be prepared to be a diagnostic kit for GII type norovirus.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and virus detection, in particular to a kit for detecting GII type norovirus and its application. Background technique [0002] Norovirus is the most important pathogen causing non-bacterial gastroenteritis outbreaks in the world. It is highly infectious and can cause small-scale outbreaks or large-scale epidemics with up to hundreds of thousands of infections. It can infect all ages Group of people. Norovirus is a virus with large genetic variation. It can be divided into five groups (Genogroup G) according to its antigenicity and nucleotide sequence variation. GI, GII, and GIV can infect humans, of which GI and GII are infectious The main genetic groups of humans can be further subdivided into different genotypes (GI.1, GI.2, GI.3, GI.4, GI.5, GI.6, GI.7, GI.8, GII.1, GII.2, GII.3, GII.4, GII.5, GII.6, GII.7, GII.8, GII.9, GII.10, GII.11, GII. 12. GII.13, GII.14, GII.15, GII.16, GI...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
Inventor 王永杰喻勇新严淑玲潘迎捷
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap