43results about How to "Short detection time" patented technology

This invention discloses a sample introduction and reaction device for detecting pesticide residues in organic solvent extracts using a liquid-phase enzyme inhibition method. The device includes a reaction tube and a cap, which are detachably assembled. The cap seals the reaction tube and contains a hollow column. A sample introduction card is sealed inside the hollow column, and the reaction tube contains enzyme reagents. This invention can be used for the liquid-phase enzyme inhibition method to detect pesticide residues extracted from organic solvents, eliminating interference from organic solvents in the enzyme inhibition detection. The device integrates the sample introduction card and enzyme reagents beforehand, sealing the sample introduction card within the hollow column for easy portability. The hollow column also serves as a support for solvent evaporation from the sample introduction card, facilitating rapid solvent evaporation. The cap and tube serve a dual purpose. This invention features a simple structure, ease of use, simple operation, short detection time, and low cost. It is suitable for on-site testing of large quantities of samples during food distribution and is easy to promote and use.

This invention belongs to the field of fungal detection technology, specifically relating to a micro-platform for rapid electrolysis and highly sensitive electrochemical nucleic acid detection of Cryptococcus neoformans and its application. The micro-platform includes an integrated microfluidic chip, an external power supply, a sample introduction system, and / or an imaging system. The integrated microfluidic chip includes an electrolysis region, an electrochemical detection region, and an electrode array region. The electrolysis region includes multiple vertically arranged counter electrodes arranged in pairs, and multiple horizontally arranged hollow channels in a serpentine pattern, bonded to the electrode pairs above them. The connection point between every two electroporated channels is the electroporation lysis point. The electrochemical detection region consists of a working electrode, a reference electrode, and a counter electrode. The working electrode is modified with rGO / AuNPs nanomaterials and molecular probes.

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.

This invention discloses a computer-aided design-based lateral flow chromatography test strip. The test strip includes a backing layer, on which a sample pad, a conjugation pad, a chromatographic membrane, and an absorption pad are sequentially and partially overlapped from one end to the other. The test strip identifies samples based on ligand enzymes, preferably using colloidal gold chromatography. RNA adapter probes are designed at both ends of the ligand enzyme using computer-aided design based on secondary structure prediction. Biotin on both the colloidal gold and the chromatographic membrane is coupled with oligonucleotide sequences that are complementary to the RNA adapter probes at both ends of the ligand enzyme, thereby achieving lateral flow chromatography detection of the sample. The chromatographic test strip of this invention is highly efficient and rapid, simplifies the screening process, reduces the number of probe optimization designs, reduces product variability, improves the versatility of the test strip platform, and improves detection sensitivity compared to existing methods.

The application discloses a specific primer, a probe combination and a method for rapidly detecting toxoplasma gondii based on RAA fluorescence method, and relates to the technical field of molecular biology detection. The specific primer sequence is shown as SEQ ID No: 2 and SEQ ID No: 10; the probe sequence is shown as SEQ ID No: 12, and is modified with a FAM-dT, THF and BHQ1-dT ternary fluorescent quenching structure and a 3' end C3-Spacer at specific positions. The primer and the probe combination can be used to complete amplification and fluorescence detection of the toxoplasma gondii WX2 gene target in 20 min under a constant temperature condition of 39 DEG C, and the sensitivity reaches 10 copies / muL. The method is suitable for rapid screening of toxoplasma gondii for non-diagnostic purposes.

The invention belongs to the technical field of sensor welding, and discloses a low-stress and low-temperature-rise laser welding process and detection method for a sensor, and the process comprises the following steps: in order to reduce deformation and stress accumulation, designing a welding joint into a symmetrical form, and designing a welding seam gap according to the same or different materials; low-power positioning welding is conducted firstly, then high-power complete welding is conducted, and the energy value of low-power laser pulses is smaller than 50% of the energy value of high-power laser pulses. The problems of stress and temperature rise caused by laser welding in the design and manufacturing process of an existing sensor are solved, and the reliability and stability of a product are improved.

This invention relates to the field of machining condition detection technology, and discloses a method for detecting chatter in high-speed milling based on a limited amount of measurement data. The method first determines the installation positions of the vibration sensor and speed sensor, and the amount of data collected per spindle revolution. n Data length for each time window nm and flutter threshold T Secondly, acquire periodically synchronized multi-point sampling data X; then, based on the amount of data collected per revolution... n The periodically synchronized multi-point sampling data is expanded into m columns of data Y; then data mining is performed on data Y, and the m columns of data are subtracted pairwise to obtain the mined m(m-1) columns of data Z; finally, the m(m-1) columns of data are calculated. Z The standard deviation of the data is used as a flutter indicator and compared with the flutter threshold T to determine whether flutter has occurred. This invention uses data mining technology to obtain more new data, shortens the time delay of flutter detection, and improves the reliability and real-time performance of flutter detection.

This invention provides a method for detecting vitamin C based on oxidase-active nanozymes. The method includes: preparing a first mixture of MnO2@MIP nanozyme, TMB solution, HAc-NaAc buffer, and the sample to be tested; and a second mixture of MnO2@MIP nanozyme, TMB solution, HAc-NaAc buffer, and vitamin C at different concentration gradients; reacting the first and second mixtures under the same predetermined reaction conditions; measuring the absorbance of the products after the reaction at predetermined wavelengths, corresponding to absorbance values ​​A1 and A0, respectively; obtaining a linear regression equation based on A0 and vitamin C at different concentration gradients; and determining the vitamin C content in the sample to be tested based on the linear regression equation and A1. The provided method is rapid and has extremely high sensitivity.

The invention discloses a method for detecting a membranous nephropathy target antigen, which comprises the following steps: adding a protein extracting solution into a glomerular tissue to be detected, and incubating; adding a mixed enzyme of a LysC enzyme and a Trypsin enzyme, and incubating; adding a dithiothreitol solution, and incubating; adding an iodo-acetamide solution, and incubating at room temperature in a dark place; adding a precipitating agent, performing ultrasonic treatment and centrifugation, taking supernate, loading the supernate to a C18 small column, eluting the small column, and collecting eluent; freezing the eluent, blow-drying the eluent, and redissolving the eluent with a loading solution to obtain a test solution; and carrying out liquid chromatography-tandem mass spectrometry detection on the test solution. By adopting the detection method disclosed by the invention, the target antigen of membranous nephropathy can be accurately detected when the sampling volume is obviously reduced, so that the sampling difficulty is greatly reduced, and the application of the laser microdissection and liquid chromatography-mass spectrometry combined technology in the detection of the target antigen of membranous nephropathy is greatly promoted. The method is simple in process and short in detection time.

This invention belongs to the field of fetoglobulin B detection technology, and discloses an immunochromatographic test strip for quantitative detection of fetoglobulin B using quantum dot fluorescent microspheres and its preparation method. The immunochromatographic test strip of this invention includes a sample pad, a release pad, and a nitrocellulose membrane; the release pad is coated with a fetoglobulin B monoclonal antibody labeled with quantum dot fluorescent microspheres and a mouse IgG antibody labeled with quantum dot fluorescent microspheres; the nitrocellulose membrane has a detection line and a control line, the detection line is coated with fetoglobulin B monoclonal antibody, and the control line is coated with goat anti-mouse IgG antibody. The immunochromatographic test strip of this invention reduces the detection time to 9-12 minutes, and the total detection time is only 20 minutes, achieving rapid and accurate quantitative detection of fetoglobulin B. It also features high detection sensitivity, a wide detection range, and simple and rapid operation, which is beneficial for the screening and monitoring of atherosclerotic coronary heart disease.

The invention relates to a method for synchronously and rapidly detecting multiple parameters of respirable dust and silicon dioxide on a membrane, and belongs to the field of dust monitoring. The method comprises the following steps: S1, carrying out on-membrane sampling and Fourier infrared spectrum scanning on the quartz respirable dust, taking the quartz respirable dust as a spectrum library of a standard sample, and establishing a standard silicon dioxide and quartz respirable dust quality prediction model based on a spectrum absorption peak of silicon dioxide at 800cm <-1 >; s2, selecting different types of dust, carrying out on-film sampling and spectrum comparative analysis, and establishing spectrum databases and dynamic correction models of different types of dust; and S3, aiming at concentration calculation deviation caused by sampling flow fluctuation, establishing a flow compensation and concentration calculation model, and finally realizing synchronous detection of multiple parameters such as respirable dust mass concentration, silicon dioxide mass concentration and silicon dioxide mass percent.

The present application relates to the fields of biotechnology and food detection technology, and discloses a rice flower honey colloidal gold immunochromatographic test strip as well as a preparation method and application thereof. Through proteomic analysis, the Ycf1 protein of chloroplast origin is identified as a specific marker of the plant source in rice flower honey, and a rice flower honey colloidal gold immunochromatographic test strip is developed. The test strip comprises a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad which are connected in sequence. The gold label pad is coated with gold-labeled antibodies formed by the combination of a monoclonal antibody against a specific segment of the Ycf1 protein and colloidal gold. The nitrocellulose membrane is provided with a detection line and a quality control line. The detection line is coated with a monoclonal antibody against the Ycf1 protein, and the quality control line is coated with a goat anti-mouse IgG. The test strip is simple to operate, and the detection can be completed within 15 minutes. The test strip has high sensitivity, strong specificity, and the results are intuitive and reliable, and is suitable for the on-site rapid screening and identification of the authenticity of rice flower honey.

The application discloses a method for identifying matcha produced by different enterprises by using fNIRS, and belongs to the field of food detection. The method detects matcha produced by different enterprises by using functional near-infrared spectroscopy (fNIRS), carries out pretreatment and discriminant analysis on full-channel characteristic wave bands, screens characteristic channels, and further uses a discriminant model established by using characteristic wave bands of the characteristic channels to accurately identify matcha produced by different enterprises. Compared with traditional sensory evaluation methods and ingredient detection technologies, the method is objective and fair, does not need to train an evaluation personnel, time consumption is shortened, and the method can be used for rapidly identifying matcha produced by different enterprises.

The application discloses a phosphorus acid triphenyl ester fluorescent quantitative rapid detection method and products and application thereof, and relates to a phosphorus acid triphenyl ester hapten, an artificial antigen, a monoclonal antibody and a preparation method thereof, and a phosphorus acid triphenyl ester fluorescent quantitative rapid detection test strip. The test strip comprises a sample pad, a label pad, a reaction membrane and a water absorption pad which are sequentially connected and fixed on a PVC bottom plate. The label pad is coated with an anti-phosphorus acid triphenyl ester monoclonal antibody which is labeled with a detectable label. The reaction membrane is provided with a T line and a C line. The T line is coated with a phosphorus acid triphenyl ester artificial antigen, and the C line is coated with a sheep anti-phosphorus acid triphenyl ester antibody. The test strip is combined with the detection method in the application, and has the advantages of high specificity, high sensitivity, short detection time, low cost, low skill requirement for an operator and the like under the premise of ensuring detection accuracy, and the detection limit reaches 0.1 ug / kg. The test strip is suitable for rapid detection of a large number of samples on site and has a good application prospect.

The present application belongs to the field of health quarantine, and relates to a borrelia burgdorferi rapid detection kit and a detection method. The kit can perform on-site rapid quantitative detection on a sample to be detected based on fluorescence microsphere immunochromatography technology. The kit comprises a borrelia burgdorferi fluorescence microsphere immunochromatography test strip. The test strip comprises a viscous bottom liner and viscous bottom plate, and is sequentially pasted with an analysis membrane, a binding pad, a water absorption pad and a sample pad. The analysis membrane is a nitrocellulose membrane, which has a detection band and a quality control band, and is respectively fixed with borrelia burgdorferi antigen protein and rabbit anti-SPA. The binding pad is fixed with an SPA and fluorescence microsphere labeled compound. The present application can realize on-site rapid quantitative detection of borrelia burgdorferi carried by mice, and has the advantages of simple operation, short detection time, high detection sensitivity, good specificity, convenient storage and easy popularization and use.

The present application relates to the technical field of detection, especially to a method for monitoring synthesis of Ile-Phe dipeptide by enzyme catalysis, which provides a method for monitoring synthesis of Ile-Phe dipeptide by enzyme catalysis, which adopts high performance liquid chromatography for detection, a C18 chromatographic column is used as the chromatographic column, mobile phase A is acetonitrile, mobile phase B is a mixture of potassium dihydrogen phosphate solution and methanol, and gradient elution is adopted. Through optimization of the chromatographic conditions, nine substances of Ile-Phe, Ile, Phe, Ile-Ile, Phe-Phe, Phe-Ile, ATP, ADP and AMP can be detected and quantified.

The application discloses a centrifugal microfluidic chip for allergen detection, comprising a chip main body and a plurality of microstructures, wherein the chip main body has a rotation center, and the microstructures comprise: a waste chamber located at the farthest end of the rotation center; a reaction detection chamber provided with a carrier, the carrier being coated with anti-human IgE antibodies; a circular truncated cone-shaped channel, the narrow end of which is connected with the other end of the reaction detection chamber, and the wide end of which is connected with a main channel; a serum separation structure comprising a blood cell chamber and a serum chamber, the serum chamber being connected with the main channel through a siphon valve; a first buffer solution chamber; an allergen chamber; a second buffer solution chamber; an enzyme storage chamber; a third buffer solution chamber; a bottom liquid chamber; and a termination liquid chamber. The application avoids contamination and evaporation of reagents, does not need additional driving equipment, is conducive to integration and miniaturization of the equipment, saves consumption of sample whole blood and reagents, reduces cost and time, and is simple to operate; the application needs small amounts of sample and reagents, has low cost, short detection time, and high detection sensitivity.

The application belongs to the field of detection, and specifically discloses a fluorescent probe, a preparation method and application thereof; the fluorescent probe comprises a compound shown in formula (I). 2+ The fluorescent probe has high sensitivity and selectivity, is not interfered by other metal ions, has a short detection time, can be detected within 30 min, can be detected completely within 30 s at most, can realize visual detection when applied to a test strip, and can be applied to the detection of Hg 2+ in environmental samples, such as actual water samples and soil samples.

A defect detection method and a defect detection apparatus are provided. The defect detection apparatus is used to detect a sample including a periodic structure. The defect detection apparatus includes a light source, an image detector and a controller. The light source is used to provide a light beam incident on the periodic structure, wherein the periodic structure converts the light beam into an interference light beam. The image detector is disposed in a path of the interference light beam, wherein the interference light beam forms an interference intensity distribution on the image detector. The controller is electrically connected to the image detector, and is used to determine whether the periodic structure has a defect by determining whether the interference intensity distribution has a normal waveform.

The invention provides a method for rapidly detecting antimony ions and application, the method for rapidly detecting antimony ions comprises the following steps: dividing a solution to be detected into two parts, adding a fluorescent probe TPE-4TA into the first part of the solution to be detected, and uniformly mixing; adding a fluorescent probe PBET into the second solution to be detected, and uniformly mixing; under excitation of light with the wavelength of 380 nm, the fluorescence intensity I460 of the first solution at the position of 460 nm and the fluorescence intensity I500 of the second solution at the position of 500 nm are measured respectively; judging the valence state of antimony ions according to the fluorescence signal; and respectively substituting the measured I460 and I500 into a pre-established Sb < 3 + > and Sb < 5 + > standard curve, and calculating to obtain the concentrations of Sb < 3 + > and Sb < 5 + > in the solution to be detected. The method has the advantages of simplicity and convenience in operation, quick reaction and high selectivity, and can realize quick detection of antimony ions with different valence states so as to meet actual environmental sample monitoring requirements.

The invention relates to a primer pair, a composition, a kit and a method for detecting pseudomonas plecoglossicida causing large yellow croaker visceral white-spot disease based on an RPA-CRISPR / Cas12a technology, the nucleotide sequences of upstream primers RPA-F are respectively as shown in SEQ ID NO.1-4, the nucleotide sequences of downstream primers RPA-R corresponding to the upstream primers RPA-F are respectively as shown in SEQ ID NO.5-8, and the composition comprises the primer pair, crRNA, Cas12a protein and a fluorescent reporter probe. Compared with the prior art, the invention establishes a P. plecoglossicida detection method which is simple and convenient to operate, short in detection time, good in specificity and high in sensitivity and only needs common constant-temperature equipment, and is suitable for on-site rapid detection in basic laboratories and large yellow croaker farms.

This invention relates to a primer and probe set and kit for multiplex fluorescent genotyping detection of norovirus strains, belonging to the field of biotechnology. The primer and probe set of this invention includes primer pairs and probes for detecting norovirus types GII.17, GII.3, and GII.4; wherein the nucleotide sequences of the primer pairs and probes for detecting GII.17 are shown in SEQ ID NO: 1-3; the nucleotide sequences of the primer pairs and probes for detecting GII.3 are shown in SEQ ID NO: 4-6; and the nucleotide sequences of the primer pairs and probes for detecting GII.4 are shown in SEQ ID NO: 7-9. This invention has the following technical advantages: 1. Short detection time and simple operation, with a total detection time of approximately 1.5 hours. 2. High sensitivity. 3. Low detection limit, applicable to environmental sample detection.

The application discloses a CRISPR-Cas12a detection primer group for Francisella tularensis and application thereof. The application provides a CRISPR-Cas12a system for detecting Francisella tularensis, and also provides RAA amplification primers for specifically amplifying the target sequence of the Francisella tularensis in the first aspect; and a probe as a fluorescent reporter molecule is also provided. The application obtains a Francisella tularensis specific gene sequence based on whole genome alignment of Francisella tularensis and its close species, further utilizes the specific gene sequence to design RAA amplification primers and crRNA sequences to detect Francisella tularensis, and develops a related kit. The application can specifically detect Francisella tularensis and distinguish it from other close species, and has the characteristics of rapidness, simplicity and accuracy.

The application relates to the technical field of traditional Chinese medicine, and particularly provides a characteristic spectrum of a Shuangge anti-diarrhea preparation and a construction method thereof. The application adopts a multi-wavelength switching technology by screening a sample processing method and optimizing chromatographic conditions, and simultaneously characterizes main efficacy components of six medicines in a prescription. The method can evaluate the overall curative effect of the Shuangge anti-diarrhea preparation from a qualitative point of view, and significantly improves the quality control level of the Shuangge anti-diarrhea preparation.

The invention provides an avian infectious bronchitis virus color visualization RAA rapid detection method, and relates to the technical field of veterinary diagnosis, and the method comprises the following steps: sample collection and treatment: collecting disease material samples representing respiratory tract symptoms, renal lesions or clinical symptoms of egg drop, extracting the samples, and carrying out sample treatment; carrying out nucleic acid extraction and reverse transcription, and carrying out RNA extraction and cDNA synthesis; carrying out conventional PCR detection; constructing a positive plasmid; the method has the advantages of rapidness, sensitivity, specificity, simplicity, convenience, low cost and the like, is suitable for rapid screening of the IBV in places with limited conditions, such as grassroots farms and veterinary stations, can be matched with laboratory nucleic acid electrophoresis verification to realize effective connection of primary screening and confirmation, and provides a new technical means for early discovery of the IBV and prevention and control of epidemic diseases.

The present application relates to the technical field of traditional Chinese medicine detection, and specifically provides a characteristic spectrum construction method of Herba Centipedae and its preparation. A specific elution procedure is obtained through a large number of experiments by using octadecylsilane bonded silica gel as a filler and a mobile phase including methanol and an acid-containing aqueous solution for gradient elution, 13 or more common characteristic peaks are obtained, and effective separation of characteristic peaks including neochlorogenic acid, caffeic acid, chlorogenic acid, cryptochlorogenic acid, 1,4-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, big pothos glycoside and thymotin lactone ketone is achieved, the peak shape is good, the baseline is smooth, the determined components can be completely separated, the detection time is short, and the method provides a basis for quality detection and control of Herba Centipedae and its preparation, can fully reflect the integrity and characteristics of Herba Centipedae and its preparation, and is simple to operate and applicable to quality control of different preparation types of Herba Centipedae.

This application provides an electrochemical sensor for detecting luteolin, comprising a working electrode and a sensing material modified on the working electrode; the sensing material is a Co-N / C nanomaterial. The Co-N / C nanomaterial prepared in this application possesses a unique structure and excellent electrocatalytic activity, which can significantly enhance the electrochemical response signal to luteolin, thereby achieving highly sensitive detection of luteolin. Simultaneously, this sensor exhibits good selectivity, effectively avoiding interference from other coexisting substances in the sample.

The present application belongs to the field of environment and food detection technology, and specifically relates to a kind of on-site fluorescent visualization detection method of antibiotic residue.The present application constructs a convenient, fast and sensitive sensing detection platform by designing fluorescent probe molecule Eu@CDs with target recognition function and combining with smart phone portable sensor.The fluorescent probe molecule Eu@CDs of the present application has blue fluorescence emission at 450 nm.When NFZ is introduced, blue light is quantitatively quenched.After adding TC, the beta-diketone group of TC is combined with Eu 3+ Chelate, absorb energy and sensitize Eu through "antenna effect" 3+ Emit 617 nm characteristic red light.Thereby, the present application realizes on-site single-probe synchronous sampling detection of NFZ and TC, and the detection limit is 0.0034 mg / L and 0.00870 mg / L respectively, and the linear range is 0.011-32.0 mg / L and 0.029-40.0 mg / L respectively, which provides an efficient and accurate solution for rapid screening of environmental and food pollutants.

The application discloses a concrete defect detection method, a detection device, an electronic device and a storage medium. The concrete defect detection method comprises the following steps: in response to a received detection instruction, acquiring a current concrete image of concrete, wherein the detection instruction is used for instructing to perform defect detection on the concrete; inputting the current concrete image into a target defect detection model to obtain a corresponding defect detection result, wherein the target defect detection model is obtained based on a single-step multi-frame target detection model and a texture enhancement model, and the texture enhancement model is used for enhancing and extracting texture features of the current concrete image. The method realizes defect detection on the concrete by using the target defect detection model constructed based on the single-step multi-frame target detection model and the texture enhancement model, the detection time is short, and the detection efficiency and detection accuracy of the defect detection on the concrete are improved.