481results about How to "Short detection time" patented technology

The invention discloses a visual detection method of New Delhi tomato leaf curl virus, and belongs to a plant virus detection technology in the field of biotechnology. The method comprises the following steps: adding genome DNA extracted from a plant sample to be detected into an RAA reaction system containing an RAA primer, and carrying out isothermal amplification; then adding an isothermal amplification product into a CRISPR / Cas12a detection system containing a specific crRNA fluorescent probe and an ssDNA fluorescent probe, and carrying out a reaction; and finally, carrying out fluorescence signal detection on the reaction product, if the fluorescence signal is positive, indicating that the New Delhi tomato leaf curl virus is detected in the plant sample, otherwise, indicating that the New Delhi tomato leaf curl virus is not detected. The method has the advantages of being good in specificity, short in detection time, high in sensitivity, free of dependence on expensive instruments and the like, and the result can be accurately detected through an instrument or directly and preliminarily interpreted in a field with limited conditions.

The invention discloses a detection method for B-type botulinum neurotoxin and application. The method disclosed by the invention is short in detection time, high in sensitivity as a qualitative detection method, capable of reaching a pictogram level, and obvious in detection advantage on trace toxin samples. Compared with an animal method using antiserum detection, the monoclonal antibody has high detection specificity, is beneficial to accurate decision-making when being used for scientific research detection and food hygiene inspection, and has small error compared with the animal method. In addition, the produced botulinum neurotoxin double-antibody sandwich reaction characteristic peak can be used for stability detection of botulinum neurotoxin, and plays a role which cannot be replaced by other methods in botulinum toxin product production, quality control, new product research and development, quality control and monitoring of standard substances for verification and market supervision. Therefore, the method can be used for guiding establishment of toxin immunodetection standard substances and promoting a capillary immunodetection technology to become a novel botulinum toxin detection standard method.

The invention relates to the field of molecular diagnosis, in particular to an RPA-Cas12a one-step detection system and application thereof. The invention finds that heparin sodium has cleavage activity for inhibiting LbCas12a, and develops a one-step nucleic acid detection method compatible with RPA amplification and Cas12 detection based on the inhibition effect. The detection limit of the method reaches 0.6 (copy / microliter), and the method can be expanded to other two Cas12 subtype proteins, namely AsCas12a and AapCas12b. In the detection of monkey pox virus, influenza A virus and new coronavirus as examples, compared with quantitative PCR, the detection sensitivity and specificity of the method both reach 95% or above. The CRISPR one-step nucleic acid detection method has the advantages of high sensitivity and specificity, short detection time and the like, and the potential nucleic acid aerosol pollution risk of a traditional two-step nucleic acid detection method is removed.

The invention discloses a yolk-based small target foreign matter detection method constructed by fusing a multi-scale sequence. The method comprises the following steps: preprocessing an obtained image; obtaining a target input image, and extracting original features of the target input image to obtain original depth features; performing hybrid convolution on the extracted original depth features to form a new total feature amount for subsequent detection training; performing multi-scale fusion on the extracted original depth features to obtain three feature maps with different scales; and according to the three feature maps with different scales, inputting a yolov5 detection head to obtain a prediction result. According to the yolk-based small target foreign matter detection method constructed by fusing the multi-scale sequence, YOLOv5 is selected as a basic network according to image characteristics, a network structure is redesigned, fine loss is reduced, light weight is guaranteed, richer gradient flow information is obtained, and therefore the detection precision and speed are guaranteed while the light weight of the network is guaranteed.

The invention discloses a nested MIRA primer combination for detecting hantavirus HTNV type and hantavirus SEOV type. The nested MIRA primer combination comprises a peripheral primer, an inner primer and a probe for detecting the hantavirus HTNV type and the hantavirus SEOV type, the peripheral primers for the hantavirus HTNV type are as shown in SEQ ID NO: 1 and SEQ ID NO: 2, the inner primers are as shown in SEQ ID NO: 3 and SEQ ID NO: 4, and the probe is as shown in SEQ ID NO: 5; the peripheral primers for the hantavirus SEOV type are as shown in SEQ ID NO: 6 and SEQ ID NO: 7, the inner primers are as shown in SEQ ID NO: 8 and SEQ ID NO: 9, and the probe is as shown in SEQ ID NO: 10; the primer disclosed by the invention can complete nested multienzyme constant-temperature nucleic acid rapid amplification within 30 minutes at the temperature of 39 DEG C, is simple and convenient to operate, has lower requirements on instruments, equipment and professional skills of operators, and can achieve the lower detection limit of 14.2 copies / mu L.

The invention belongs to the technical field of hydraulic engineering, and provides a sediment particle size distribution measuring system and method.The method comprises the steps that images of sediment particles in a water body are collected, and a first sediment particle image is obtained; processing the first sediment particle image to obtain a sediment grayscale image; a K-means clustering algorithm is adopted to carry out clustering analysis on the sediment gray level image, and an image with a first-level clustering result is obtained; carrying out clustering analysis on sediment particles with particle sizes smaller than a preset threshold value in the image with the first-level clustering result by adopting a DBSCN clustering algorithm to obtain an image with a second-level clustering result; and carrying out edge detection on the sediment particles in the image with the secondary clustering result, and then sequentially carrying out binarization processing, equivalent circle diameter transformation and statistics on the particle size distribution of the sediment particles to obtain a particle size distribution result. The problem that an existing sediment particle size measurement system is low in measurement precision in a complex underwater environment with dense sediment and more fine particles is solved.

The invention discloses a sewage heavy metal ion detection device, which belongs to the technical field of sewage detection, and comprises a sensor module and a microfluidic module, the sensor module comprises a Loff wave surface acoustic wave device, the Loff wave surface acoustic wave device comprises a resonance area and two Loff wave interdigital transducers, the resonance area is located between the two Loff wave interdigital transducers, and the resonance area is covered with a heavy metal ion adsorption film; the micro-fluidic module comprises a micro-channel system and a Rayleigh wave surface acoustic wave device, the micro-channel system comprises a dropwise adding tank, a micro-channel and a waste liquid tank which are communicated in sequence, the micro-channel is provided with an experimental area overlapped with the resonance area, and the Rayleigh wave surface acoustic wave device is used for driving sewage to move from the dropwise adding tank to the waste liquid tank. By combining a microfluidic technology and a surface acoustic wave technology, heavy metal ions, especially lead ions, in sewage can be rapidly, accurately and conveniently monitored.

The invention provides a photoelectrochemical biosensor based on a porous membrane as well as a preparation method and application of the photoelectrochemical biosensor, and belongs to the field of photoelectrochemical biosensors. The photoelectrochemical biosensor comprises an electrode, the electrode comprises ITO conductive glass and a composite film coated on the ITO conductive glass, the composite film is obtained by modifying a photosensitive material in a nano-pore channel of a porous film and then connecting a capture probe in the nano-pore channel, and the photoelectrochemical biosensor based on the porous film is obtained. The sensor has the following advantages: 1, other biomolecules can be detected by changing the type of the capture probe, and the sensor has excellent practical application value; 2, the ITO conductive glass is loaded with the porous membrane to replace a traditional metal electrode, so that the detection is accurate, the detection time is short, the cost is low, and the reproducibility and the stability are good;

The invention discloses a space cabin door system and a rapid leak rate detection method thereof, and belongs to the technical field of reliability and safety design of space cabin doors. The cabin door system comprises a sealed cabin door, a pressure point switch and a cabin door rapid leak detector, the end face of a door body of the sealed cabin door is sleeved with a sealing ring inner ring and a sealing ring outer ring, when the cabin door is closed, the two sealing rings make contact with the sealing face of the door frame in a pressed mode, and a sealed cavity is formed between the two sealing rings and the sealing face of the door frame. The pressure point switch is arranged on the door frame and detects whether the cabin door switch acts in place or not. And the cabin door rapid leak detector inflates the sealed cavity and performs leak rate test to obtain the sealing condition of the sealing ring. Through the application of the invention, the in-place action of the cabin door can be reliably detected, the sealing performance of the cabin door can be quickly given, and a quantitative basis is provided for reliably closing the cabin door.

The invention provides a hybrid creatine kinase isoenzyme double-antibody sandwich ELISA kit and a preparation method thereof, and belongs to the technical field of CK-MB antibody and creatine kinase isoenzyme quantitative detection. The process comprises the following steps: respectively immunizing Japanese big-ear rabbits and mice by using CK-MB heterodimer protein as an immunogen, screening positive monoclonal cells by adopting a Beacon single-cell screening platform, and preparing a rabbit (mouse) anti-human CK-MB gene engineering antibody; the double-antibody sandwich ELISA detection kit is established by further screening optimal reaction conditions. The detection method disclosed by the invention has excellent performance in the aspects of intra-batch and inter-batch repeatability, sensitivity and specificity, and is suitable for CK-MB detection in clinical and scientific research.

The invention relates to a fluorescent array sensor, in particular to a difunctional fluorescent array sensor containing a modified high-molecular polymer, a preparation method of the difunctional fluorescent array sensor and application of the difunctional fluorescent array sensor in bacterial detection and fermentation carbohydrate detection. The array sensor comprises at least two different fluorescent sensing units, each fluorescent sensing unit is a PN-coumarin dye compound formed by covalent interaction of PN and coumarin dye, PN is a nitrophenylboronic acid modified high-molecular polymer PEI, the coumarin dye is 6, 7-diphenyl-1, 3, 4-triazole-3-carboxylic acid, and the coumarin dye is a coumarin dye compound. The compound is selected from the group consisting of 2, 7-dihydroxy-2H-benzopyran-2-one ES or 4-methylesculetin MS. The compound fluorescent array sensor disclosed by the invention can simultaneously realize distinguishing detection of thirteen types of bacteria related to IBS and fourteen FODMAPs, and has the advantages of high sensitivity, short detection time, low cost, good repeatability, high accuracy, no need of professional technicians and the like.

The invention discloses a bimodal polymyxin drug-resistant gene MCR-1 detection platform, relates to the field of food safety and analytical chemistry, and in particular relates to an on-site rapid detection method of the polymyxin drug-resistant gene MCR-1 in a food sample, primer groups aiming at the polymyxin drug-resistant gene MCR-1 are respectively as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5, and a sixth primer with the 5'end labeled by biotin; the CRISPR / dCas9 system comprises dCas9 protein and sgRNA, and the sequence of the sgRNA is marked as SEQ ID NO.7; the sequence of the gold-platinum nanoprobe is marked as SEQ ID NO.8; the LAMP technology is adopted to amplify the polymyxin drug-resistant gene MCR-1 in the sample to be detected, an LAMP product contains multiple sections of reverse circulation repetitive sequences, and each double strand can be combined with more gold-platinum nanoparticles, so that sensitive detection of the food-borne pathogenic bacteria containing the polymyxin drug-resistant gene MCR-1 in the food sample is realized, the sensitivity is relatively high, the detection time is short, the detection time is short, and the cost is low. The operation is simple, the stability is high, and the whole detection process can be completed in a short time.

The invention belongs to the field of chemical medicine analysis, and particularly relates to a method for determining the content of impurities in phloroglucinol trimethyl ether. According to the method provided by the invention, the excessive methylation impurities (2, 4, 6-trimethoxytoluene, 2, 4, 6-trimethoxyxylene and 2, 4, 6-trimethoxytrimethylbenzene) generated in the methylation process can be detected, and the excessive methylation impurities are prevented from being further transferred and existing in a phloroglucinol trimethyl ether raw material medicine and a final product phloroglucinol injection preparation. Researches on specificity, accuracy, precision, limit of detection, limit of quantitation, linear range, durability and the like of the method prove that the method is suitable for measuring the content of the over-methylated impurities in the phloroglucinol trimethyl ether.

The invention discloses a crystal form judgment method of a silicon carbide single crystal polymorphic symbiotic region and application, and belongs to the technical field of silicon carbide material detection. The determination method comprises the following steps: (1) testing: carrying out Raman test on silicon carbide single crystals to obtain a test spectrogram, and classifying characteristic peaks in the test spectrogram into first crystal form Raman characteristic peaks and second crystal form Raman characteristic peaks according to Raman characteristic peaks of different crystal forms; and (2) judgment: judging that the crystal form at the test point is the first crystal form, a symbiotic crystal form of the first crystal form and the second crystal form or the second crystal form according to the ratio of the peak intensity of the Raman characteristic peak of the first crystal form to the peak intensity of the Raman characteristic peak of the second crystal form. According to the method, Raman is adopted to test the silicon carbide single crystal material, and the crystal form at a test point is judged according to a test spectrogram, so that the crystal form of a multi-type symbiotic region of the silicon carbide single crystal material can be accurately distinguished and quantitatively characterized, and the method can be used for large-scale detection of a silicon carbide single crystal rod.

The invention provides a construction method and application of a portable rapid visual tobacco mosaic virus paper-based molecular imprinting sensor. According to the sensor, tobacco mosaic virus (TMV) is taken as a template, high-specificity organic fluorescent nanogel imprinted polymer TMV-nanoMIPs obtained by solid-phase imprinting is taken as signal output, meanwhile, a cheap and portable paper base is taken as a sensing chip, TMV is imprinted on the paper base, and the paper-based imprinted polymer WP-coated MIPs with a TMV imprinted cavity is obtained after elution. After the WP-coated MIPs specifically capture the TMV, the TMV-nanoMIPs also can be combined to the TMV due to the specificity of the TMV-nanoMIPs, so that a sandwich structure of the MIPs-TMV-MIPs is formed. The TMV-nanoMIPs are used as the signal probe, the rapid semi-quantitative visual detection of the target virus TMV by naked eyes and an ultraviolet lamp is realized, the obvious change can be observed within ten minutes, and meanwhile, the TMV-nanoMIPs can be quantitatively detected and analyzed through a fluorospectro photometer. And the method has good detection capability in tobacco leaves actually infected by TMV, and the cost of the method is as low as 0.623 yuan / piece. The sensor is expected to realize on-site self-service rapid visual detection when crops are infected with viruses, and has important significance on prevention and control of plant diseases and pests.

The invention belongs to the technical field of biology, and particularly relates to a real-time fluorescent quantitative PCR (polymerase chain reaction) detection kit for porcine reproductive and respiratory syndrome virus and application of the real-time fluorescent quantitative PCR detection kit. The invention designs a primer probe combination for detecting porcine reproductive and respiratory syndrome virus, the primer probe combination consists of a primer P1, a primer P2 and a probe, and the sequences of the primer P1, the primer P2 and the probe are respectively shown as a sequence 1, a sequence 2 and a sequence 3. The invention further provides a kit containing the primer probe combination. The kit can be used for identifying and detecting the porcine reproductive and respiratory syndrome virus. The primer P1 and the primer P2 designed by the invention have strong specificity and high sensitivity, the detection sensitivity of the primer P1 and the primer P2 to the porcine reproductive and respiratory syndrome virus reaches 1.0 * 10 < 1 > copies / mu L, the detection repeatability is good, and the primer P1 and the primer P2 can be used for rapidly detecting the porcine reproductive and respiratory syndrome virus.

The invention provides a method for rapidly detecting various antibiotics in a water body. The preparation method comprises the following steps: firstly, preparing an enhanced substrate for surface enhanced Raman spectroscopy analysis, and enabling the substrate to have high SERS activity by a method for re-dispersing large aggregates through salt regulation and ultrasonic treatment; secondly, preparing a'glass-PDMS (Polydimethylsiloxane)-glass' micro-fluidic chip; the enhanced substrate and a sample to be detected are premixed and then pumped into the micro-fluidic chip for SERS (Surface Enhanced Raman Scattering) detection. According to the invention, ultralow-concentration detection of multiple antibiotics in multiple water bodies can be realized; the method has the advantages of no need of marking, short detection time and high sensitivity, and can be used for rapid and high-precision detection of various antibiotics in a water body.

The invention discloses a corn seed nondestructive testing method based on an infrared imaging technology, which comprises the following steps: adding distilled water into a germination box so that the water surface just contacts each to-be-tested corn seed, overlooking and collecting an infrared thermal imaging image in a germination disc, subtracting the reference water temperature mean value from the temperature mean value at the same time for collecting the infrared thermal imaging image to obtain a difference value, and substituting the difference value and the time for collecting the infrared thermal imaging image into a coordinate system to form a temperature difference change curve; converting the filtered temperature difference change curve of the to-be-tested corn seed into a one-dimensional array, taking the one-dimensional array as a training data set, performing label classification on the training data set, and inputting the training data set into a support vector machine (SVM) model for training to obtain a trained SVM model; and inputting the test data set into the trained SVM model for prediction to obtain a prediction result of the test data set. After the corn seed nondestructive testing method is used for testing, the corn seeds to be tested can be continuously planted and used after being dried.

The invention relates to the field of molecular biology and clinical medicine, and relates to a double-split aptamer probe and application thereof. The double-split aptamer probe comprises two split aptamers, namely a split aptamer 1 and a split aptamer 2, the split aptamer 1 comprises an aptamer fragment 1 and a split initiator 1, the split aptamer 2 comprises an aptamer fragment 2 and a split initiator 2, and the two aptamer fragments are from a complete aptamer sequence A. The invention further discloses a preparation method of the double-split aptamer probe. The invention proves that DNA (deoxyribonucleic acid) can be successfully self-assembled in a solution to form a fluorescence tree with larger molecular weight under the triggering of a target, so that signal amplification is realized. The constructed sandwich-type fluorescence sensor can be successfully applied to Ade detection, in the concentration range of 0.01-100 ng / mL, the detected fluorescence intensity is enhanced along with increase of the concentration of Ade, LOD is not higher than 5.23 pg / mL, the detection time is not longer than 30 min, and the sandwich-type fluorescence sensor has the advantages of being high in sensitivity and short in detection time.

The invention discloses a kit and a detection method for detecting an internally-running sheep virus epidemic strain, the kit comprises a crRNA sequence and an RT-RPA primer, the detection method comprises an RT-RPA reaction system and a CRISPR / cas12a reaction system, RT-RPA nucleic acid amplification and CRISPR / cas12a nucleic acid detection are combined, a two-step detection method is adopted, firstly, RT-RPA nucleic acid amplification is carried out, secondly, RT-RPA nucleic acid amplification is carried out, thirdly, RT-RPA nucleic acid amplification is carried out, secondly, CRISPR / cas12a nucleic acid detection is carried out, and thirdly, RT-RPA nucleic acid detection is carried out. Then, the RT-RPA nucleic acid product is added into a CRISPR / cas12a nucleic acid detection system, and the Chinese epidemic strain of the internally-running sheep virus can be detected. The detection method is simple, efficient, low in dependence on instruments and equipment, good in sensitivity, specificity and stability and good in social application prospect.

The invention belongs to the technical field of drug analysis and detection, and particularly relates to a method for simultaneously detecting three isomer impurities in a macloxvir raw material and a preparation thereof and application. The method can be used for simultaneously detecting three isomer impurities in the macarovir raw material and the preparation thereof under one chromatographic condition, the separation degree of each chromatographic peak is relatively good, the detection limit and the quantitation limit also meet the qualitative and quantitative analysis requirements of medicines, the sensitivity is high, the precision and the stability are relatively high, the reproducibility is good, the accuracy is high, and the method is suitable for industrial production. The method can be used for monitoring the impurities in the production process of the macloxvir, and is beneficial to control and improvement of the quality of the macloxvir; meanwhile, operation is easy, detection time is short, and the method is very suitable for quality monitoring of raw material medicines in large-scale industrial production.

The invention belongs to the technical field of in-vitro diagnosis and rapid detection, and relates to an interleukin 6 and procalcitonin joint detection kit, a preparation method and a detection method, the interleukin 6 and procalcitonin joint detection kit comprises a Ra1 reagent, a Ra2 reagent, an Rb1 reagent, an Rb2 reagent and a substrate solution; the Ra1 reagent is an interleukin 6 antibody magnetic bead labeling solution, and is prepared from a biotin labeled interleukin 6 first antibody solution and a streptavidin magnetic bead solution; the Ra2 reagent is a procalcitonin antibody magnetic bead labeling solution, and is prepared from a biotin labeled procalcitonin first antibody solution and a streptavidin magnetic bead solution; the Rb1 reagent is prepared from an alkaline phosphatase labeled interleukin 6 second antibody solution; the Rb2 reagent is prepared from a second procalcitonin antibody solution labeled by horse radish peroxidase; the substrate solution comprises a first substrate solution and a second substrate solution. The detection sensitivity can be improved, the detection time is shortened, the detection efficiency is improved, and the operation is simple.

The invention relates to the technical field of traditional Chinese medicine detection, and particularly provides a construction method of specific chromatograms of common carpesium fruit and a preparation thereof. Comprising the following steps: by taking octadecylsilane chemically bonded silica as a filling agent and a mobile phase comprising methanol and an acid-containing aqueous solution, carrying out gradient elution, and screening through a large number of experiments to obtain a specific elution procedure, so as to obtain 13 or more common characteristic peaks, and realizing the purification of the chlorogenic acid, the caffeic acid, the chlorogenic acid, the cryptochlorogenic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid and the 1, 4-dicaffeoylquinic acid. The method can effectively separate characteristic peaks of 2, 5-dicaffeoylquinic acid, cosmosiin and carabrone, is good in peak shape, stable in base line, short in detection time, provides a basis for quality detection and control of the common carpesium fruit and the preparation thereof, can fully reflect the integrity and characteristics of the common carpesium fruit and the preparation thereof, is simple to operate, and is suitable for industrial production. The method is suitable for quality control of different preparation types of common carpesium fruit.

The invention belongs to the technical field of molecular imprinting fluorescence sensing, and discloses a ratio type core-shell molecular imprinting quantum dot fluorescence encoding microsphere, a preparation method and application. The preparation method comprises the following steps: firstly, mixing a metal organic framework material ZIF-8 with green quantum dots for primary coding to obtain a carrier ZIF-8 green quantum dot fluorescence coding material, synthesizing a norfloxacin molecular imprinting shell layer on the surface of the carrier, and then mixing with red quantum dots for secondary coding to obtain the ratio type core-shell molecular imprinting quantum dot fluorescence coding microspheres. And by combining with an organic solvent, the constructed related sensor is used for rapid detection of norfloxacin. The method disclosed by the invention has the advantages that the core-shell structure can avoid interference of two quantum dots on fluorescence properties caused by contact, and the existence of the ZIF-8 core material can increase the specific surface area of the encoding microspheres, accelerate the mass transfer rate, greatly shorten the detection time and improve the detection accuracy. And a new solution is provided for rapid quantitative detection of norfloxacin in a food matrix.

The invention relates to the technical field of optical module circuit boards, and particularly discloses a high-transmission-rate optical module circuit board and a method. Filling shape and structural feature extraction is carried out on the through hole filling state image by adopting an image analysis and processing technology based on deep learning; and automatically judging whether a defect exists or not by using a feature representation obtained by performing feature selection on the through hole state structure feature based on the through hole filling state shape feature as a constraint feature. Thus, the through hole filling state image is automatically collected through the industrial camera, the detection time can be remarkably shortened, and compared with manual detection or X-ray and ultrasonic detection conducted on samples one by one, the higher detection speed can be achieved. Meanwhile, tiny defects such as tiny bubbles, cracks and layering can be recognized, the detection accuracy and reliability are improved, and automatic heat conduction through hole filling quality detection is achieved.

The invention belongs to the technical field of biology, and particularly relates to a reagent and a kit for detecting eosinophilic granulocyte chronic sinusitis with nasal polyp. The detection reagent comprises the following components: a substance which is used for detecting the Galectin-10 protein (Charcot-Leyden Crystal, CLCs) in a crystalline state, and a detection reagent which is used for detecting the Galectin-10 protein (Charcot-Leyden Crystal, CLCs) in the crystalline state. The reagent for detecting CLCs comprises a mixture of eosin, glacial acetic acid and water. The mass volume ratio of eosin to glacial acetic acid is 1: (1-50) g / mL, and the volume ratio of glacial acetic acid to water is 1: (2-100). According to the invention, the reagent capable of efficiently detecting the CLCs is found for the first time, and the CLCs is a biomarker of the eosinophilic chronic sinusitis with nasal polyp, so that the detection reagent for the eosinophilic chronic sinusitis with nasal polyp is provided. By detecting CLCs, eosinophilic granulocyte chronic sinusitis with nasal polyp can be predicted, and the method is simple, convenient and fast and has good prediction performance.

The invention discloses a method for measuring the concentration of various dissolved phosphorus forms in industrial sewage. The method comprises the following steps: S1, preparing a plurality of mixed solutions with different concentrations and containing various phosphorus forms in a dissolved state, and respectively carrying out ion chromatographic analysis on the mixed solution with each concentration; establishing a standard curve of each phosphorus form according to the concentration of each phosphorus form and the chromatographic peak of each phosphorus form in the ion chromatogram corresponding to the concentration; the multiple phosphorus forms comprise orthophosphate radicals, pyrophosphate radicals, trimetaphosphate radicals and tripolyphosphate radicals; s2, adjusting the pH value of a to-be-detected industrial sewage sample to be neutral, enabling the to-be-detected industrial sewage sample to sequentially pass through the column 1, the column 2 and the column 3, and then performing ion chromatographic analysis on the water sample passing through the columns to obtain an ion chromatogram of the water sample; and obtaining the concentration of each phosphorus form in the water sample according to the ion chromatogram of the water sample and the standard curve of each phosphorus form. The purpose of automatically analyzing the concentrations of various phosphates in the industrial sewage is achieved, operation is easy, and accuracy is high.

The invention discloses a method for detecting lacosamide enantiomers by using reversed-phase liquid chromatography, which can directly and effectively separate lacosamide from the enantiomers, has good detection result accuracy and high sensitivity, is simpler to operate, easier to balance, has lower cost, is more environmentally friendly, and has a shorter analysis time than the existing normal-phase chromatography, can be directly used for the detection of solution preparations such as lacosamide injection, and is more convenient to operate, thereby providing a better guarantee for the quality control of lacosamide and its preparations.

The invention discloses a method for detecting the characteristic chromatogram of granules for relieving depression and soothing nerves, which specifically comprises the following steps of: performing chromatographic condition and system applicability test: performing gradient elution by taking octadecylsilane chemically bonded silica as a filler, acetonitrile as a mobile phase A and 0.1% phosphoric acid as a mobile phase B, and detecting the wavelength of 210 nm; preparing a test solution; preparing a reference substance solution; respectively and precisely sucking the reference substance solution and the test solution and injecting the reference substance solution and the test solution into a high performance liquid chromatograph for determination, analysis and the like. The method disclosed by the invention is simple to operate, strong in specificity, short in detection time and accurate in detection result, can be used for quickly identifying the depression-resolving and nerve-soothing granules, can be used for comprehensively and quickly evaluating the quality of the depression-resolving and nerve-soothing granules, is beneficial to quality identification and quality control of the depression-resolving and nerve-soothing granules, and is suitable for popularization and application. Therefore, the safety and effectiveness of clinical medication are ensured.

The invention belongs to the field of environment and food pollution detection, and relates to a microcystic toxin-LR (MC-LR) bimodal detection method based on regulation and control of MIP-Cu at CDs laccase activity. Copper chloride dihydrate, citric acid and ethidene diamine are used as synthetic substrates, a fluorescent nano-enzyme with laccase-like activity is synthesized in one step through a hydrothermal method, and the MC-LR can be specifically recognized and the laccase-like activity of MIP-Cu at CDs can be regulated and controlled through wrapping of a molecular imprinting method, so that a colorimetric and fluorescent dual-mode rapid MC-LR determination method is established. The method is short in detection time, high in sensitivity and low in cost. Built-in cross reference correction is provided by dual modes, so that the MC-LR detection reliability and accuracy in a complex environment sample are greatly improved, and false positive output of a detection result is avoided.