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A Micro-Platform for Rapid Electrolysis and High-Sensitivity Electrochemical Nucleic Acid Detection of Cryptococcus and Its Application
ActiveCN117607222BRapid lysisavoid pollutionMaterial electrochemical variablesElectrolysisElectroporation
This invention belongs to the field of fungal detection technology, specifically relating to a micro-platform for rapid electrolysis and highly sensitive electrochemical nucleic acid detection of Cryptococcus neoformans and its application. The micro-platform includes an integrated microfluidic chip, an external power supply, a sample introduction system, and / or an imaging system. The integrated microfluidic chip includes an electrolysis region, an electrochemical detection region, and an electrode array region. The electrolysis region includes multiple vertically arranged counter electrodes arranged in pairs, and multiple horizontally arranged hollow channels in a serpentine pattern, bonded to the electrode pairs above them. The connection point between every two electroporated channels is the electroporation lysis point. The electrochemical detection region consists of a working electrode, a reference electrode, and a counter electrode. The working electrode is modified with rGO / AuNPs nanomaterials and molecular probes.
Owner:BEIHANG UNIV +1
Specific primer, probe combination and method for detecting toxoplasma gondii based on raa fluorescence method
ActiveCN121915179BSimple and fast operationshorten detection timeMicrobiological testing/measurementMicroorganism based processesGene targetsGondii toxoplasma
The application discloses a specific primer, a probe combination and a method for rapidly detecting toxoplasma gondii based on RAA fluorescence method, and relates to the technical field of molecular biology detection. The specific primer sequence is shown as SEQ ID No: 2 and SEQ ID No: 10; the probe sequence is shown as SEQ ID No: 12, and is modified with a FAM-dT, THF and BHQ1-dT ternary fluorescent quenching structure and a 3' end C3-Spacer at specific positions. The primer and the probe combination can be used to complete amplification and fluorescence detection of the toxoplasma gondii WX2 gene target in 20 min under a constant temperature condition of 39 DEG C, and the sensitivity reaches 10 copies / muL. The method is suitable for rapid screening of toxoplasma gondii for non-diagnostic purposes.
Owner:CENT SOUTH UNIV
A borrelia burgdorferi rapid detection kit and detection method
The present application belongs to the field of health quarantine, and relates to a borrelia burgdorferi rapid detection kit and a detection method. The kit can perform on-site rapid quantitative detection on a sample to be detected based on fluorescence microsphere immunochromatography technology. The kit comprises a borrelia burgdorferi fluorescence microsphere immunochromatography test strip. The test strip comprises a viscous bottom liner and viscous bottom plate, and is sequentially pasted with an analysis membrane, a binding pad, a water absorption pad and a sample pad. The analysis membrane is a nitrocellulose membrane, which has a detection band and a quality control band, and is respectively fixed with borrelia burgdorferi antigen protein and rabbit anti-SPA. The binding pad is fixed with an SPA and fluorescence microsphere labeled compound. The present application can realize on-site rapid quantitative detection of borrelia burgdorferi carried by mice, and has the advantages of simple operation, short detection time, high detection sensitivity, good specificity, convenient storage and easy popularization and use.
Owner:DALIAN INT TRAVEL HEALTH CARE CENT (DALIAN CUSTOMS PORT CLINIC)
Method for monitoring synthesis of ile-phe dipeptide by enzyme catalysis
The present application relates to the technical field of detection, especially to a method for monitoring synthesis of Ile-Phe dipeptide by enzyme catalysis, which provides a method for monitoring synthesis of Ile-Phe dipeptide by enzyme catalysis, which adopts high performance liquid chromatography for detection, a C18 chromatographic column is used as the chromatographic column, mobile phase A is acetonitrile, mobile phase B is a mixture of potassium dihydrogen phosphate solution and methanol, and gradient elution is adopted. Through optimization of the chromatographic conditions, nine substances of Ile-Phe, Ile, Phe, Ile-Ile, Phe-Phe, Phe-Ile, ATP, ADP and AMP can be detected and quantified.
Owner:SHENZHEN READLINE BIOTECH CO LTD
Inspection tooling
ActiveCN224286301UEasy installation timeEasy detection timeMeasurement of fluid loss/gain rateMechanical engineeringPhysics
Owner:ZHEJIANG KANGSHENG HEAT EXCHANGER CO LTD
A fluorescent probe and a preparation method and application thereof
ActiveCN119930497Bincrease brightnessHigh fluorescence emission propertiesOrganic chemistryFluorescence/phosphorescenceFluoProbesFluorescence
The application belongs to the field of detection, and specifically discloses a fluorescent probe, a preparation method and application thereof; the fluorescent probe comprises a compound shown in formula (I). 2+ The fluorescent probe has high sensitivity and selectivity, is not interfered by other metal ions, has a short detection time, can be detected within 30 min, can be detected completely within 30 s at most, can realize visual detection when applied to a test strip, and can be applied to the detection of Hg 2+ in environmental samples, such as actual water samples and soil samples.
Owner:SOUTH CHINA NORMAL UNIV
Defect detection method and defect detection apparatus
PendingCN122259465AImprove detection rateshort detection timePhase-affecting property measurementsScattering properties measurementsLight beamImage detector
A defect detection method and a defect detection apparatus are provided. The defect detection apparatus is used to detect a sample including a periodic structure. The defect detection apparatus includes a light source, an image detector and a controller. The light source is used to provide a light beam incident on the periodic structure, wherein the periodic structure converts the light beam into an interference light beam. The image detector is disposed in a path of the interference light beam, wherein the interference light beam forms an interference intensity distribution on the image detector. The controller is electrically connected to the image detector, and is used to determine whether the periodic structure has a defect by determining whether the interference intensity distribution has a normal waveform.
Owner:NAN YA TECH
Characteristic spectrum of shuangge antidiarrheal preparation and construction method thereof
PendingCN122259768Ahigh sensitivityImprove detection efficiencyComponent separationCurative effectEfficacy
The application relates to the technical field of traditional Chinese medicine, and particularly provides a characteristic spectrum of a Shuangge anti-diarrhea preparation and a construction method thereof. The application adopts a multi-wavelength switching technology by screening a sample processing method and optimizing chromatographic conditions, and simultaneously characterizes main efficacy components of six medicines in a prescription. The method can evaluate the overall curative effect of the Shuangge anti-diarrhea preparation from a qualitative point of view, and significantly improves the quality control level of the Shuangge anti-diarrhea preparation.
Owner:LUOYANG HUIZHONG ANIMAL MEDICINE
Electrochemical sensor for detecting luteolin and preparation and detection method thereof
PendingCN122282900Aunique structureImprove electrocatalytic activityElectrochemical responseLuteolin
This application provides an electrochemical sensor for detecting luteolin, comprising a working electrode and a sensing material modified on the working electrode; the sensing material is a Co-N / C nanomaterial. The Co-N / C nanomaterial prepared in this application possesses a unique structure and excellent electrocatalytic activity, which can significantly enhance the electrochemical response signal to luteolin, thereby achieving highly sensitive detection of luteolin. Simultaneously, this sensor exhibits good selectivity, effectively avoiding interference from other coexisting substances in the sample.
Owner:HONGHE UNIVERSITY
miRNA detection system, kit, and detection method based on DNA aggregation-induced precipitation as a signal output.
PendingCN122081468Ahigh sensitivityStrong specificityMicrobiological testing/measurementMaterial analysis by optical meansSpecific detectionA-DNA
This invention discloses a miRNA detection system, kit, and detection method based on DNA aggregation-induced precipitation as a signal output. The detection system comprises: a DNA triangle, a hairpin-structured probe H1, a hairpin-structured probe H2, and a DNA double-stranded linker with sticky ends. Probes H1 and H2 can generate DNA double-stranded strands H1-H2 with sticky ends at both ends through a catalytic hairpin self-assembly reaction. Probe H1 contains a sequence complementary to the miRNA sequence to be detected. The DNA triangle has sticky ends at its first and second corners, complementary to both ends of the DNA double-stranded linker, and a sticky end at its third corner, complementary to both ends of DNA double-stranded strands H1-H2. This invention achieves highly sensitive and specific detection of miRNAs without the need for complex instruments and equipment, making it suitable for on-site, real-time detection.
Owner:NORTH SICHUAN MEDICAL COLLEGE
A quadruple RT-qPCR method and kit for simultaneously detecting canine gastrointestinal virus and application thereof
PendingCN122105005Arapid identificationhigh sensitivityMicrobiological testing/measurementMicroorganism based processesRotavirusBioinformatics
The application discloses a quadruple RT-qPCR method for simultaneously detecting canine digestive tract viruses, a kit and application, and the real-time fluorescent quantitative PCR detection kit comprises PCR primer probes, the PCR primer probes comprise forward PCR amplification primers, reverse PCR amplification primers and detection probes respectively for canine parvovirus, canine coronavirus, canine adenovirus type 1 and canine rotavirus; the sequences are shown in SEQ ID NO. 1 to SEQ ID NO. 12, respectively, the application can detect canine parvovirus, canine coronavirus, canine adenovirus type 1 and canine rotavirus, has very high sensitivity and specificity, and is favorable for early diagnosis or treatment of sick dogs.
Owner:NANJING ZHUOYI BIOTECHNOLOGY CO LTD
A competitive monoclonal antibody against bovine ephemeral fever virus and its application in constructing a competitive ELISA kit
PendingCN122080192Arapid diagnosisaccurate diagnosisImmunoglobulins against virusesBiological testingElisa kitHeavy chain
This invention discloses a competitive monoclonal antibody against bovine ephemeral fever virus (BOF) and its application in constructing a competitive ELISA kit, belonging to the field of monoclonal antibody technology. The technical problem this invention aims to solve is how to accurately detect BEF. This invention provides a competitive monoclonal antibody against BEF, wherein the amino acid sequence of the heavy chain variable region of the monoclonal antibody is shown in SEQ ID NO.2; and the amino acid sequence of the light chain variable region of the monoclonal antibody is shown in SEQ ID NO.7. This enables rapid and accurate diagnosis of BEF, facilitating timely implementation of prevention and control measures and reducing the risk of epidemic transmission.
Owner:HARBIN VETERINARY RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES (CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENTER HARBIN BRANCH CENTER)