123results about How to "Short detection time" patented technology

The invention discloses a nested MIRA primer combination for detecting hantavirus HTNV type and hantavirus SEOV type. The nested MIRA primer combination comprises a peripheral primer, an inner primer and a probe for detecting the hantavirus HTNV type and the hantavirus SEOV type, the peripheral primers for the hantavirus HTNV type are as shown in SEQ ID NO: 1 and SEQ ID NO: 2, the inner primers are as shown in SEQ ID NO: 3 and SEQ ID NO: 4, and the probe is as shown in SEQ ID NO: 5; the peripheral primers for the hantavirus SEOV type are as shown in SEQ ID NO: 6 and SEQ ID NO: 7, the inner primers are as shown in SEQ ID NO: 8 and SEQ ID NO: 9, and the probe is as shown in SEQ ID NO: 10; the primer disclosed by the invention can complete nested multienzyme constant-temperature nucleic acid rapid amplification within 30 minutes at the temperature of 39 DEG C, is simple and convenient to operate, has lower requirements on instruments, equipment and professional skills of operators, and can achieve the lower detection limit of 14.2 copies / mu L.

The invention belongs to the technical field of hydraulic engineering, and provides a sediment particle size distribution measuring system and method.The method comprises the steps that images of sediment particles in a water body are collected, and a first sediment particle image is obtained; processing the first sediment particle image to obtain a sediment grayscale image; a K-means clustering algorithm is adopted to carry out clustering analysis on the sediment gray level image, and an image with a first-level clustering result is obtained; carrying out clustering analysis on sediment particles with particle sizes smaller than a preset threshold value in the image with the first-level clustering result by adopting a DBSCN clustering algorithm to obtain an image with a second-level clustering result; and carrying out edge detection on the sediment particles in the image with the secondary clustering result, and then sequentially carrying out binarization processing, equivalent circle diameter transformation and statistics on the particle size distribution of the sediment particles to obtain a particle size distribution result. The problem that an existing sediment particle size measurement system is low in measurement precision in a complex underwater environment with dense sediment and more fine particles is solved.

The invention discloses a sewage heavy metal ion detection device, which belongs to the technical field of sewage detection, and comprises a sensor module and a microfluidic module, the sensor module comprises a Loff wave surface acoustic wave device, the Loff wave surface acoustic wave device comprises a resonance area and two Loff wave interdigital transducers, the resonance area is located between the two Loff wave interdigital transducers, and the resonance area is covered with a heavy metal ion adsorption film; the micro-fluidic module comprises a micro-channel system and a Rayleigh wave surface acoustic wave device, the micro-channel system comprises a dropwise adding tank, a micro-channel and a waste liquid tank which are communicated in sequence, the micro-channel is provided with an experimental area overlapped with the resonance area, and the Rayleigh wave surface acoustic wave device is used for driving sewage to move from the dropwise adding tank to the waste liquid tank. By combining a microfluidic technology and a surface acoustic wave technology, heavy metal ions, especially lead ions, in sewage can be rapidly, accurately and conveniently monitored.

The invention provides a hybrid creatine kinase isoenzyme double-antibody sandwich ELISA kit and a preparation method thereof, and belongs to the technical field of CK-MB antibody and creatine kinase isoenzyme quantitative detection. The process comprises the following steps: respectively immunizing Japanese big-ear rabbits and mice by using CK-MB heterodimer protein as an immunogen, screening positive monoclonal cells by adopting a Beacon single-cell screening platform, and preparing a rabbit (mouse) anti-human CK-MB gene engineering antibody; the double-antibody sandwich ELISA detection kit is established by further screening optimal reaction conditions. The detection method disclosed by the invention has excellent performance in the aspects of intra-batch and inter-batch repeatability, sensitivity and specificity, and is suitable for CK-MB detection in clinical and scientific research.

The invention relates to a fluorescent array sensor, in particular to a difunctional fluorescent array sensor containing a modified high-molecular polymer, a preparation method of the difunctional fluorescent array sensor and application of the difunctional fluorescent array sensor in bacterial detection and fermentation carbohydrate detection. The array sensor comprises at least two different fluorescent sensing units, each fluorescent sensing unit is a PN-coumarin dye compound formed by covalent interaction of PN and coumarin dye, PN is a nitrophenylboronic acid modified high-molecular polymer PEI, the coumarin dye is 6, 7-diphenyl-1, 3, 4-triazole-3-carboxylic acid, and the coumarin dye is a coumarin dye compound. The compound is selected from the group consisting of 2, 7-dihydroxy-2H-benzopyran-2-one ES or 4-methylesculetin MS. The compound fluorescent array sensor disclosed by the invention can simultaneously realize distinguishing detection of thirteen types of bacteria related to IBS and fourteen FODMAPs, and has the advantages of high sensitivity, short detection time, low cost, good repeatability, high accuracy, no need of professional technicians and the like.

The invention belongs to the field of chemical medicine analysis, and particularly relates to a method for determining the content of impurities in phloroglucinol trimethyl ether. According to the method provided by the invention, the excessive methylation impurities (2, 4, 6-trimethoxytoluene, 2, 4, 6-trimethoxyxylene and 2, 4, 6-trimethoxytrimethylbenzene) generated in the methylation process can be detected, and the excessive methylation impurities are prevented from being further transferred and existing in a phloroglucinol trimethyl ether raw material medicine and a final product phloroglucinol injection preparation. Researches on specificity, accuracy, precision, limit of detection, limit of quantitation, linear range, durability and the like of the method prove that the method is suitable for measuring the content of the over-methylated impurities in the phloroglucinol trimethyl ether.

The invention belongs to the technical field of biology, and particularly relates to a real-time fluorescent quantitative PCR (polymerase chain reaction) detection kit for porcine reproductive and respiratory syndrome virus and application of the real-time fluorescent quantitative PCR detection kit. The invention designs a primer probe combination for detecting porcine reproductive and respiratory syndrome virus, the primer probe combination consists of a primer P1, a primer P2 and a probe, and the sequences of the primer P1, the primer P2 and the probe are respectively shown as a sequence 1, a sequence 2 and a sequence 3. The invention further provides a kit containing the primer probe combination. The kit can be used for identifying and detecting the porcine reproductive and respiratory syndrome virus. The primer P1 and the primer P2 designed by the invention have strong specificity and high sensitivity, the detection sensitivity of the primer P1 and the primer P2 to the porcine reproductive and respiratory syndrome virus reaches 1.0 * 10 < 1 > copies / mu L, the detection repeatability is good, and the primer P1 and the primer P2 can be used for rapidly detecting the porcine reproductive and respiratory syndrome virus.

The invention provides a method for rapidly detecting various antibiotics in a water body. The preparation method comprises the following steps: firstly, preparing an enhanced substrate for surface enhanced Raman spectroscopy analysis, and enabling the substrate to have high SERS activity by a method for re-dispersing large aggregates through salt regulation and ultrasonic treatment; secondly, preparing a'glass-PDMS (Polydimethylsiloxane)-glass' micro-fluidic chip; the enhanced substrate and a sample to be detected are premixed and then pumped into the micro-fluidic chip for SERS (Surface Enhanced Raman Scattering) detection. According to the invention, ultralow-concentration detection of multiple antibiotics in multiple water bodies can be realized; the method has the advantages of no need of marking, short detection time and high sensitivity, and can be used for rapid and high-precision detection of various antibiotics in a water body.

The invention discloses a kit and a detection method for detecting an internally-running sheep virus epidemic strain, the kit comprises a crRNA sequence and an RT-RPA primer, the detection method comprises an RT-RPA reaction system and a CRISPR / cas12a reaction system, RT-RPA nucleic acid amplification and CRISPR / cas12a nucleic acid detection are combined, a two-step detection method is adopted, firstly, RT-RPA nucleic acid amplification is carried out, secondly, RT-RPA nucleic acid amplification is carried out, thirdly, RT-RPA nucleic acid amplification is carried out, secondly, CRISPR / cas12a nucleic acid detection is carried out, and thirdly, RT-RPA nucleic acid detection is carried out. Then, the RT-RPA nucleic acid product is added into a CRISPR / cas12a nucleic acid detection system, and the Chinese epidemic strain of the internally-running sheep virus can be detected. The detection method is simple, efficient, low in dependence on instruments and equipment, good in sensitivity, specificity and stability and good in social application prospect.

The invention belongs to the technical field of in-vitro diagnosis and rapid detection, and relates to an interleukin 6 and procalcitonin joint detection kit, a preparation method and a detection method, the interleukin 6 and procalcitonin joint detection kit comprises a Ra1 reagent, a Ra2 reagent, an Rb1 reagent, an Rb2 reagent and a substrate solution; the Ra1 reagent is an interleukin 6 antibody magnetic bead labeling solution, and is prepared from a biotin labeled interleukin 6 first antibody solution and a streptavidin magnetic bead solution; the Ra2 reagent is a procalcitonin antibody magnetic bead labeling solution, and is prepared from a biotin labeled procalcitonin first antibody solution and a streptavidin magnetic bead solution; the Rb1 reagent is prepared from an alkaline phosphatase labeled interleukin 6 second antibody solution; the Rb2 reagent is prepared from a second procalcitonin antibody solution labeled by horse radish peroxidase; the substrate solution comprises a first substrate solution and a second substrate solution. The detection sensitivity can be improved, the detection time is shortened, the detection efficiency is improved, and the operation is simple.

The invention belongs to the technical field of biology, and particularly relates to a reagent and a kit for detecting eosinophilic granulocyte chronic sinusitis with nasal polyp. The detection reagent comprises the following components: a substance which is used for detecting the Galectin-10 protein (Charcot-Leyden Crystal, CLCs) in a crystalline state, and a detection reagent which is used for detecting the Galectin-10 protein (Charcot-Leyden Crystal, CLCs) in the crystalline state. The reagent for detecting CLCs comprises a mixture of eosin, glacial acetic acid and water. The mass volume ratio of eosin to glacial acetic acid is 1: (1-50) g / mL, and the volume ratio of glacial acetic acid to water is 1: (2-100). According to the invention, the reagent capable of efficiently detecting the CLCs is found for the first time, and the CLCs is a biomarker of the eosinophilic chronic sinusitis with nasal polyp, so that the detection reagent for the eosinophilic chronic sinusitis with nasal polyp is provided. By detecting CLCs, eosinophilic granulocyte chronic sinusitis with nasal polyp can be predicted, and the method is simple, convenient and fast and has good prediction performance.

The invention discloses a method for measuring the concentration of various dissolved phosphorus forms in industrial sewage. The method comprises the following steps: S1, preparing a plurality of mixed solutions with different concentrations and containing various phosphorus forms in a dissolved state, and respectively carrying out ion chromatographic analysis on the mixed solution with each concentration; establishing a standard curve of each phosphorus form according to the concentration of each phosphorus form and the chromatographic peak of each phosphorus form in the ion chromatogram corresponding to the concentration; the multiple phosphorus forms comprise orthophosphate radicals, pyrophosphate radicals, trimetaphosphate radicals and tripolyphosphate radicals; s2, adjusting the pH value of a to-be-detected industrial sewage sample to be neutral, enabling the to-be-detected industrial sewage sample to sequentially pass through the column 1, the column 2 and the column 3, and then performing ion chromatographic analysis on the water sample passing through the columns to obtain an ion chromatogram of the water sample; and obtaining the concentration of each phosphorus form in the water sample according to the ion chromatogram of the water sample and the standard curve of each phosphorus form. The purpose of automatically analyzing the concentrations of various phosphates in the industrial sewage is achieved, operation is easy, and accuracy is high.

The invention relates to the technical field of biological detection, in particular to a kit and method for detecting NfL, the kit comprises a magnetic bead coated with a capture antibody, a non-functional auxiliary magnetic bead, an NfL detection antibody coupled with a first marker, beta-galactosidase coupled with a second marker, an enzyme reaction luminescent substrate and an NfL standard substance; the capture antibody in the magnetic bead coated with the capture antibody is used for being specifically combined with an NfL antigen; the first marker in the NfL detection antibody coupled with the first marker is connected with the second marker in the beta-galactosidase coupled with the second marker, and the NfL detection antibody is suitable for being specifically combined with an NfL antigen. The kit provided by the invention has the advantages of higher sensitivity, less background signal interference, wider linear range, convenience for full-automatic detection instruments and the like.

This invention discloses a sample introduction and reaction device for detecting pesticide residues in organic solvent extracts using a liquid-phase enzyme inhibition method. The device includes a reaction tube and a cap, which are detachably assembled. The cap seals the reaction tube and contains a hollow column. A sample introduction card is sealed inside the hollow column, and the reaction tube contains enzyme reagents. This invention can be used for the liquid-phase enzyme inhibition method to detect pesticide residues extracted from organic solvents, eliminating interference from organic solvents in the enzyme inhibition detection. The device integrates the sample introduction card and enzyme reagents beforehand, sealing the sample introduction card within the hollow column for easy portability. The hollow column also serves as a support for solvent evaporation from the sample introduction card, facilitating rapid solvent evaporation. The cap and tube serve a dual purpose. This invention features a simple structure, ease of use, simple operation, short detection time, and low cost. It is suitable for on-site testing of large quantities of samples during food distribution and is easy to promote and use.

The invention provides a nichrome etching agent and a nichrome grain corrosion display method, the nichrome etching agent comprises a mixed solution of hydrofluoric acid, nitric acid and water, and the volume ratio of hydrofluoric acid to nitric acid to water is 1: (9-11): (18-22). When the nickel-chromium alloy etchant is used for chemical corrosion, crystal grains are clearly displayed, the metallographic display range is wide, product analysis is more accurate, the process is stable and simple, the nickel-chromium alloy etchant can be repeatedly used, the detection time is short, a result can be quickly obtained, and unqualified products are effectively prevented from being produced.

The invention discloses a KASP primer composition, a reaction system and application of the KASP primer composition in detection of an ALDH2 gene rs671 polymorphic site, and relates to the technical field of biology. According to the invention, a KASP typing technology which is more popular in recent years is adopted, a universal probe is used in the KASP technology and is located in a reaction system, and detection can be carried out only by synthesizing a common amplification primer during detection. Two pairs of universal probes are respectively complementary with a first tag sequence and a second tag sequence, so that amplification products of different genotypes are subjected to fluorophore labeling, and different SNPs correspond to different fluorescence signals. And a typing result can be obtained by collecting and analyzing a fluorescence signal. The primer composition and the kit provided by the invention can realize accurate, rapid, simple and convenient detection of the SNP at the rs671 site of the ALDH2 gene, have the advantages of high sensitivity, strong specificity, simple operation procedure, short detection time and the like, and have wide application prospects.

The invention relates to the field of fluorescent nanomaterials, and in particular to a fluorescent probe for detecting gatifloxacin and an application thereof in food. The invention uses bear bile powder as a precursor to prepare a novel biomatrix-derived carbon quantum dot, and constructs a highly selective fluorescent sensing platform, which is successfully used to detect gatifloxacin in milk and honey. The prepared carbon quantum dots show obvious concentration dependence. Under sunlight, as the concentration of the carbon quantum dots increases, the color of the solution gradually changes from light yellow to orange-yellow, and under ultraviolet light, it shows a transition from blue fluorescence to green fluorescence. By establishing a linear relationship between the fluorescence signal intensity and the gatifloxacin concentration, sensitive detection of gatifloxacin is achieved, and the detection limit can be as low as 3 μg / L, which proves the excellent performance of the fluorescent probe and provides a certain reference value for the application of biomatrix-derived carbon quantum dots in the field of food safety detection.

The present invention discloses a preparation method and application of a cypermethrin nano-antibody-colloidal gold marker. The present invention provides a preparation method of a cypermethrin nano-antibody-colloidal gold marker, and the prepared cypermethrin nano-antibody-colloidal gold marker has high sensitivity and strong stability. The nano-antibody-colloidal gold test strip for detecting cypermethrin pesticide provided by the present invention has the advantages of high sensitivity, strong specificity, low cost, simple operation, short detection time, rapid detection suitable for the market, easy storage, and long shelf life. The nano-antibody-colloidal gold test strip prepared by the present invention has a sensitivity of 0.013-0.309 mg / kg in the residual detection of cypermethrin pesticide, and the reaction time is 5-8 min. The method for detecting the residue of cypermethrin pesticide is simple, rapid, intuitive, accurate, widely applicable, low cost, easy to promote and use, and is suitable for the screening and on-site monitoring of a large number of samples on the market.

The invention belongs to the technical field of avian reticuloendotheliosis virus detection, and particularly relates to an avian reticuloendotheliosis virus detection primer, a probe, a kit and a detection method. Comprising a primer and a probe, the primer sequence is shown as SEQ ID NO: 1 and SEQ ID NO: 2, and the probe sequence is shown as SEQ ID NO: 3. The primer, the probe and the detection method provided by the invention have the advantages of high sensitivity and specificity, good repeatability, simplicity and convenience in operation, short detection time, small required sample size, minimum detection concentration of 1.77 * 10 copies / mu L, low pollution, cost saving and the like, can be used for directly detecting DNA extracted from an unknown sample, can be complementary with a traditional detection scheme, and can be used for detecting the DNA in the unknown sample. The kit has a high application value in rapid detection of viruses, and provides a basis for clinical diagnosis of REV.

This invention belongs to the field of fungal detection technology, specifically relating to a micro-platform for rapid electrolysis and highly sensitive electrochemical nucleic acid detection of Cryptococcus neoformans and its application. The micro-platform includes an integrated microfluidic chip, an external power supply, a sample introduction system, and / or an imaging system. The integrated microfluidic chip includes an electrolysis region, an electrochemical detection region, and an electrode array region. The electrolysis region includes multiple vertically arranged counter electrodes arranged in pairs, and multiple horizontally arranged hollow channels in a serpentine pattern, bonded to the electrode pairs above them. The connection point between every two electroporated channels is the electroporation lysis point. The electrochemical detection region consists of a working electrode, a reference electrode, and a counter electrode. The working electrode is modified with rGO / AuNPs nanomaterials and molecular probes.

The utility model relates to the technical field of worm gear and worm meshing center distance measurement, in particular to a worm gear and worm meshing center distance measuring mechanism which comprises a supporting seat, a limiting mechanism is arranged at the top of the supporting seat, a worm is arranged in the limiting mechanism, one end of the worm is provided with a mounting seat, and the other end of the worm is provided with a clamping mechanism. A motor is arranged at the top of the mounting base, the output of the motor is fixedly connected with a coupler, a detection mechanism is arranged on one side of the limiting mechanism, and a display screen is arranged on one side of the detection mechanism. The worm gear and worm meshing center distance measuring mechanism is provided with the limiting mechanism, the worm can be limited through the limiting mechanism and can be rapidly replaced, and the worm gear and worm meshing center distance measuring mechanism is provided with the detection mechanism and the moving mechanism which are matched with each other. Whether the center distance of the worm gear and the worm during meshing is qualified or not can be conveniently, rapidly and accurately detected, so that the working efficiency is improved.

Compared with the prior art, the visual inspection equipment for the sealing ring comprises a rack; the conical surface rotating body assembly is arranged on the rack, and the conical surface rotating body assembly is used for bearing and rotating a sealing ring; the visual detection assembly is arranged on the rack and used for detecting the inner diameter and the outer diameter of the sealing ring; the Z-axis movement assembly is arranged on the rack; and the roundness correction assembly is arranged on the Z-axis movement assembly, located above the conical surface rotating body assembly and used for positioning and fixing the sealing ring. Compared with the prior art, the visual inspection equipment for the sealing ring, provided by the invention, has the advantages that products of various specifications can be accurately detected, the detection and manufacturing cost is lower, the detection time is shorter, and the efficiency is higher.

The invention discloses a sensor material based on a lanthanide metal organic framework as well as a preparation method and application thereof, and belongs to the technical field of white spirit detection. The sensor material based on the lanthanide metal organic framework is a composite material formed by adding lanthanide metal terbium or europium into the metal organic framework for modification, and is an Eu-MOF (at) Eu-MOF material, a Tb-MOF (at) Tb-MOF material or an Eu-MOF (at) Tb-MOF material. A fluorescent sensing array with 11 sensing channels is constructed by taking Eu-MOF (at) Eu-MOF, Tb-MOF (at) Tb-MOF or Eu-MOF (at) Tb-MOF material as a probe, so that the organic substances and the Luzhou-flavor liquor are detected, and the fluorescent sensing array has the advantages of high sensitivity, short detection time, short response time, good portability and the like, and is very suitable for rapid detection of the Luzhou-flavor liquor.

The invention belongs to the field of analytical chemistry, and particularly relates to an analysis method for detecting multiple residual solvents in a medicine preparation process. According to the method, 21 organic solvents including methanol, tetrahydrofuran, ethanol, cyclohexane, diethyl ether, isopropyl acetate, acetone, n-heptane, isopropanol, n-butyl alcohol, acetonitrile, dioxane, dichloromethane, pyridine, tert-butyl alcohol, toluene, tert-butyl methyl ether, N, N-dimethylformamide, n-hexane, dimethyl sulfoxide and ethyl acetate are simultaneously detected by adopting a gas chromatographic method. The method is not influenced by drug types, can be applied to content detection of various drug residual solvents, is simple to operate and wide in application, and saves drug detection time and detection cost.

The present invention discloses a bPAG detection method based on aptamer sandwich-RPA reaction and its application. The detection method comprises an aptamer that can specifically bind to bovine pregnancy-associated glycoprotein (bPAG) as shown in SEQ ID No.1, a forward primer as shown in SEQ ID No.2, and a reverse primer as shown in SEQ ID No.3. Based on the sandwich principle, the aptamer is used as a recognition probe and an amplification template to perform a recombinase polymerase isothermal amplification reaction, and the signal is directly or indirectly amplified by a luminescent substance or biotin on the amplified product, and bPAG is qualitatively and quantitatively detected. The bPAG detection method provided by the present invention is simple to operate, has low detection cost, high detection sensitivity, and short detection time, and can be used for laboratory and field detection of early pregnancy in dairy cows.

The invention provides a liquid gate graphene transistor sensor capable of rapidly detecting a urine marker EN2 of prostatic cancer. The liquid gate graphene transistor comprises a substrate, and a gate electrode, a source electrode and a drain electrode which are arranged on the substrate; a graphene channel is arranged between the source electrode and the drain electrode, a long-chain aptamer with the strongest affinity with a target EN2 protein is determined according to the target EN2 protein, and the aptamer is fixed on the surface of a gold grid electrode of the transistor through an Au-S bond. When the EN2 protein is detected, the long-chain aptamer can be folded under the action of an object to be detected, the EN2 protein is positively charged in 0.1 * PBS, so that the liquid gate graphene transistor generates an output signal, high-sensitivity detection of the EN2 protein is realized, the low detection limit of 0.1 fg / m is realized, the detection time is 19 minutes, and the clinical urine sample detection accuracy reaches 100%. The method can be used for detecting the marker in the urine of the prostatic cancer, the marker can be obtained without an invasive mode, and noninvasive detection on the prostatic cancer is expected to be realized.

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.

The method for identifying the salinity of the living environment of the grass carp through the fish meat comprises the following steps: firstly, quantitatively analyzing and detecting volatile components in back muscles of the grass carp; and then, according to a quantitative analysis detection result, identifying and judging the salinity of the living environment of the grass carp. By utilizing the method disclosed by the invention, the grass carp cultured in fresh water and the grass carp cultured in saline water with different salinity can be quickly and accurately identified.

The invention discloses a reagent for detecting African swine fever virus gene type II and application of the reagent. The reagent comprises a primer group, the primer group comprises a forward primer with a sequence as shown in SEQ ID NO: 2 and a reverse primer with a sequence as shown in SEQ ID NO: 3. Compared with other similar kits, the kit for detecting the African swine fever gene type II, provided by the invention, has the advantages that the sensitivity can be remarkably improved through the design of the primers, the specificity is high, the sensitivity is high, the amplification efficiency is high, the detection time is short and the like; according to the kit provided by the invention, a user does not need to carry out reagent split charging, the extracted sample nucleic acid is directly added into the pre-split freeze-dried reagent to be detected in a fluorescent PCR instrument, and the operation is simple and rapid.

The invention discloses a method for detecting the mixing uniformity of steel slag improved soil, and belongs to the technical field of highway construction. The method comprises the following steps: carrying out infrared irradiation on the steel slag improved high liquid limit soil by adopting an infrared heating device; an infrared imager is adopted to observe and photograph the steel slag improved high-liquid-limit soil which is heated by infrared irradiation or cooled after irradiation is stopped; and performing real-time judgment on the mixing uniformity of the steel slag in the steel slag improved high liquid limit soil area irradiated by the infrared rays according to the color difference. Compared with the prior art, the method disclosed by the invention has the following beneficial effects that (1) the mixing uniformity of the improved soil and the steel slag is judged by utilizing the obvious difference between the heat conductivity coefficient and the specific heat of the steel slag and the high liquid limit soil, so that direct visual misjudgment is avoided; and (2) the method provided by the invention is simple and easy to implement, the detection time is short, consumables are not needed, and the method has remarkable popularization and application values.